LOC104924175 cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC104924175
Entrez Gene ID	104924175
Full Name	rhodopsin kinase 2-like
Gene Type	protein-coding
Organism	Larimichthys crocea(large yellow croaker)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010737439.2	XP_010735741.2	rhodopsin kinase 2-like
XM_010737439.3	XP_010735741.3	rhodopsin kinase 2

Pathways from BioSystems
Gene Ontology

The following LOC104924175 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC104924175 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLa221821	XM_010737439.3 Latest version!	Larimichthys crocea rhodopsin kinase 2 (LOC104924175), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OLa90066	XM_010737439.2 Latest version!	Larimichthys crocea rhodopsin kinase 2-like (LOC104924175), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC104924175 ( XM_010737439.3 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010735741.3 Larimichthys crocea rhodopsin kinase 2 (LOC104924175), mRNA.

Price & Availability↑

CloneID	OLa221821
Clone ID Related Accession (Same CDS sequence)	XM_010737439.3
Accession Version	XM_010737439.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	rhodopsin kinase 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040017.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_010737439.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTCAAAGA AAACATTCAA TACGAGCGAC CTGTTGGGAT TGGACAACCT GGTGGCCAAC 
ACGTCCTACC TGAGGGCTCA GAATTTGGAT CGCAGTGAGC TGAGGAAGCT AAGACTCACC 
CTGTCGCTGC CCAAACCAAA GAAATCCTCT GAACTAGAGT TGGCGGTGGG GGGGAAGTAC 
GTGTCACTGT GTGAGCAGCA GCCTATTGGG AGGAAGTTGT TCCAGCAGTT TCTCCAGTCC 
TCTAACCTGC AGTATGTGGC CGCTGCTGAG TTCCTGGAGG AGCTGAGTCA CTGGAGCTTT 
GCTGAAGATG AAACTAAAGA GGAGGCCAAA CGGAGCATCC TCGCTAAGTT CTGCCAACCT 
GAATCCAAAA GTTTCCTCTC CTATCTCACA GGAGAGGAAG CTCAAAGATG CAAGGCGTTA 
TCAGACAAGA ACTTTAATGA GGAGACAATA AACCAGATAA AAGAAGCCAC GAGGGACTTC 
CTGAGAGGGA AACCTTTCTC GGAGTATCTG AAAAGCCCCT TTTTTTATAA GTTCCTGCAG 
TGGAAGGAGT ACGAGAAGCA GAAGATCACT AGGAGATACT TTTATGAGTT TAGGACATTG 
GGAAAAGGGG GCTTTGGCGA GGTGTGTGCA GTGCAGGTGA AACACACAGG CCAAATGTAT 
GCCTGCAAGA AGCTGGATAA GAGACGCTTG AAGAAGAAAG GTGGCGAGAG GTTAGCTCTT 
CTGGAGAAGC AGGTCCTGGA GAAGGTCGAC AGCCTCTTTA TCGTAAACTT GGCCTACACT 
TTCGACACCA AGATCCACCT GTGCCTAGTC ATGGACCTCA TGAACGGAGG AGACCTCAGG 
TTCCATATTT ACGAGCTTGG GGAGCGGGGT ATCCGTATGG ATCGTGTTGT TTACTACACG 
GCACAGATAA CCACTGGGAT ACTCCACCTG CACTCCCTGG ACATTGTGTA CCGTGATATG 
AAGCCTGAGA ACGTGCTCCT GGACGCTAAA GGGCAGTGTC GGCTGTCAGA CCTCGGGTTG 
GCTGTGGAGC TGCCAAAAGG CAAAACAATC TGCCAGATGG CTGGTACGAC AGGTTATATG 
GCTCCTGAGA TTCTGAGGCA GGACAACTAC AACTCATCTG TGGACTGGTG GGCTCTGGGC 
TGCAGCATCT ATGAGATGGT GGCCGCTCGT TTGCCTTTCA AAGACTTCAG AGAGAAAGTG 
CAGAACGAGG AGGTGACTCG CCGCACTCTG GAGGAGGAGT GCAAGTTTGA ACACAAACGC 
TTTGATGCTC CCACCAAAGA TATCATCAGC CGTTTTCTCA GGAAGAAGGC ACAGCATCGC 
CTTGGGAGCC GGGGTGATGA TCCACGAAAA AACCCATTTT TCAAAAGCGT CAATTTCCTT 
CGTCTGGAGG CCGGACTAGT GGAGCCCCCC TGGGTGCCAA AATCCAACGT GGTCTACGCC 
ATGAACATGA ACACATTCAG GGACAGCACT GAGGTGGAAA ACATTAAGTT CAATGACAAG 
GATGAGAAAT TCTTCAAGGA GTTCAGCACA GGTGCCGTCT CCGTGAAGTG GCAGAAGGAG 
ATGATTGACA GTGGACTCTT TGACGAACTG AACGACCCCC AACAGAACGG CCACGGCTGT 
GAGACTGTGG AGACATCCAG GGCGTGCATC ATTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010735741.3
CDS	1..1659
Translation	MSKKTFNTSDLLGLDNLVANTSYLRAQNLDRSELRKLRLTLSLPKPKKSSELELAVGGKYVSLCEQQPIGRKLFQQFLQSSNLQYVAAAEFLEELSHWSFAEDETKEEAKRSILAKFCQPESKSFLSYLTGEEAQRCKALSDKNFNEETINQIKEATRDFLRGKPFSEYLKSPFFYKFLQWKEYEKQKITRRYFYEFRTLGKGGFGEVCAVQVKHTGQMYACKKLDKRRLKKKGGERLALLEKQVLEKVDSLFIVNLAYTFDTKIHLCLVMDLMNGGDLRFHIYELGERGIRMDRVVYYTAQITTGILHLHSLDIVYRDMKPENVLLDAKGQCRLSDLGLAVELPKGKTICQMAGTTGYMAPEILRQDNYNSSVDWWALGCSIYEMVAARLPFKDFREKVQNEEVTRRTLEEECKFEHKRFDAPTKDIISRFLRKKAQHRLGSRGDDPRKNPFFKSVNFLRLEAGLVEPPWVPKSNVVYAMNMNTFRDSTEVENIKFNDKDEKFFKEFSTGAVSVKWQKEMIDSGLFDELNDPQQNGHGCETVETSRACIIL

Target ORF information:

RefSeq Version	XM_010737439.3
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea rhodopsin kinase 2 (LOC104924175), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010737439.3

ORF Insert Sequence:

ATGTCAAAGA AAACATTCAA TACGAGCGAC CTGTTGGGAT TGGACAACCT GGTGGCCAAC 
ACGTCCTACC TGAGGGCTCA GAATTTGGAT CGCAGTGAGC TGAGGAAGCT AAGACTCACC 
CTGTCGCTGC CCAAACCAAA GAAATCCTCT GAACTAGAGT TGGCGGTGGG GGGGAAGTAC 
GTGTCACTGT GTGAGCAGCA GCCTATTGGG AGGAAGTTGT TCCAGCAGTT TCTCCAGTCC 
TCTAACCTGC AGTATGTGGC CGCTGCTGAG TTCCTGGAGG AGCTGAGTCA CTGGAGCTTT 
GCTGAAGATG AAACTAAAGA GGAGGCCAAA CGGAGCATCC TCGCTAAGTT CTGCCAACCT 
GAATCCAAAA GTTTCCTCTC CTATCTCACA GGAGAGGAAG CTCAAAGATG CAAGGCGTTA 
TCAGACAAGA ACTTTAATGA GGAGACAATA AACCAGATAA AAGAAGCCAC GAGGGACTTC 
CTGAGAGGGA AACCTTTCTC GGAGTATCTG AAAAGCCCCT TTTTTTATAA GTTCCTGCAG 
TGGAAGGAGT ACGAGAAGCA GAAGATCACT AGGAGATACT TTTATGAGTT TAGGACATTG 
GGAAAAGGGG GCTTTGGCGA GGTGTGTGCA GTGCAGGTGA AACACACAGG CCAAATGTAT 
GCCTGCAAGA AGCTGGATAA GAGACGCTTG AAGAAGAAAG GTGGCGAGAG GTTAGCTCTT 
CTGGAGAAGC AGGTCCTGGA GAAGGTCGAC AGCCTCTTTA TCGTAAACTT GGCCTACACT 
TTCGACACCA AGATCCACCT GTGCCTAGTC ATGGACCTCA TGAACGGAGG AGACCTCAGG 
TTCCATATTT ACGAGCTTGG GGAGCGGGGT ATCCGTATGG ATCGTGTTGT TTACTACACG 
GCACAGATAA CCACTGGGAT ACTCCACCTG CACTCCCTGG ACATTGTGTA CCGTGATATG 
AAGCCTGAGA ACGTGCTCCT GGACGCTAAA GGGCAGTGTC GGCTGTCAGA CCTCGGGTTG 
GCTGTGGAGC TGCCAAAAGG CAAAACAATC TGCCAGATGG CTGGTACGAC AGGTTATATG 
GCTCCTGAGA TTCTGAGGCA GGACAACTAC AACTCATCTG TGGACTGGTG GGCTCTGGGC 
TGCAGCATCT ATGAGATGGT GGCCGCTCGT TTGCCTTTCA AAGACTTCAG AGAGAAAGTG 
CAGAACGAGG AGGTGACTCG CCGCACTCTG GAGGAGGAGT GCAAGTTTGA ACACAAACGC 
TTTGATGCTC CCACCAAAGA TATCATCAGC CGTTTTCTCA GGAAGAAGGC ACAGCATCGC 
CTTGGGAGCC GGGGTGATGA TCCACGAAAA AACCCATTTT TCAAAAGCGT CAATTTCCTT 
CGTCTGGAGG CCGGACTAGT GGAGCCCCCC TGGGTGCCAA AATCCAACGT GGTCTACGCC 
ATGAACATGA ACACATTCAG GGACAGCACT GAGGTGGAAA ACATTAAGTT CAATGACAAG 
GATGAGAAAT TCTTCAAGGA GTTCAGCACA GGTGCCGTCT CCGTGAAGTG GCAGAAGGAG 
ATGATTGACA GTGGACTCTT TGACGAACTG AACGACCCCC AACAGAACGG CCACGGCTGT 
GAGACTGTGG AGACATCCAG GGCGTGCATC ATTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104924175 ( XM_010737439.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010735741.2 Larimichthys crocea rhodopsin kinase 2-like (LOC104924175), mRNA.

Price & Availability↑

CloneID	OLa90066
Clone ID Related Accession (Same CDS sequence)	XM_010737439.2
Accession Version	XM_010737439.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	rhodopsin kinase 2-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017608254.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 29, 2016 this sequence version replaced XM_010737439.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTCAAAGA AAACATTCAA TACGAGTGAC CTGTTGGGAT TGGACAACCT GGTGGCCAAC 
ACGTCCTACC TGAGGGCTCA GAATTTGGAT CGCAGTGAGC TGAGGAAGCT GAGACTCACC 
CTGTCGCTGC CCAAACCAAA GAAATCCTCT GAACTAGAGT TGGCAGTGGG GGGGAAGTAC 
GTGTCACTGT GTGAGCAGCA GCCTATTGGG AGGAAGTTGT TCCAGCAGTT TCTCCAGTCC 
TCTAACCTGC AGTATGTGGC CGCTGCTGAG TTCCTGGAGG AGCTGAGTCA CTGGAGCTTT 
GCTGAAGATG AAACTAAAGA GGAGGCCAAA CGGAGCATCC TCGCTAAGTT CTGCCAACCT 
GAATCCAAAA GTTTCCTCTC CTATCTCACA GGAGAGGAAG CTCAAAGATG CAAGGCGTTA 
TCAGACAAGA ACTTTAATGA GGAGACAATA AACCAGATAA AAGAAGCCAC GAGGGACTTC 
CTGAGAGGGA AACCTTTCTC GGAGTATCTG AAAAGCCCCT TTTTTTATAA GTTCCTGCAG 
TGGAAGGAGT ACGAGAAGCA GAAGATCACT AGGAGATACT TTTATGAGTT TAGGACATTG 
GGAAAAGGGG GCTTTGGCGA GGTGTGTGCA GTGCAGGTGA AACACACAGG CCAAATGTAT 
GCCTGCAAAA AGCTGGATAA GAGACGCTTG AAGAAGAAAT GTGGCGAGAG GTTAGCTCTT 
CTGGAGAAGC AGGTCCTGGA GAAGGTCGAC AGCCTCTTTA TCGTAAACTT GGCCTATGCT 
TTCGACACCA AGACCCACCT GTGCCTAGTC ATGGACCTCA TGAACGGAGG AGACCTCAGG 
TTCCATATTT ACGAGCTCGG GGAGCGGGGT ATCCGTATGG ATCGTGTTGT TTACTACACG 
GCACAGATAA CCACTGGGAT ACTCCACCTG CACTCCCTGG ACATTGTGTA CCGTGATATG 
AAGCCTGAGA ACGTGCTCCT GGACGCTAAA GGGCAGTGTC GGCTGTCAGA CCTCGGGTTG 
GCTGTGGAGC TGCCAAAAGG CAAAACAATC TGCCAGATGG CTGGTACGAC AGGTTATATG 
GCTCCTGAGA TTCTGAGGCA GGACAACTAC AACTCATCTG TGGACTGGTG GGCTCTGGGC 
TGCAGCATCT ATGAGATGGT GGCCGCTCGT TTGCCTTTCA AAGACTTCAG AGAGAAAGTG 
CAGAACGAGG AGGTGACTCG CCGCACTCTG GAGGAGGAGT GCAAGTTTGA ACACAAACGC 
TTTGATGCTC CCACCAAAGA TATCATCAGC CGTTTTCTCA GGAAGAAGGC ACAGCATCGC 
CTTGGGAGCC GGGGTGATGA TCCACGAAAA AACCCATTTT TCAAAAGCAT CAATTTCCTT 
CGTCTGGAGG CCGGACTGGT GGAGCCCCCC TGGGTGCCAA AATCCAACGT GGTCTACGCC 
ATGAACATGA ACACGTTCAG GGACAGCACT GAGGTGGAAA ACATTAAGTT CAATGACAAG 
GATGAGAAAT TCTTCAAGGA GTTCAGCACA GGTGCCGTCT CCGTGAAGTG GCAGAAGGAG 
ATGATTGACA GTGGACTCTT TGACGAACTG AACGACCCCC AACAGAACGG CCACAGCTGT 
GAGACTGTGG AGACATCCAG GGCGTGCATC ATTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010735741.2
CDS	1..1659
Translation	MSKKTFNTSDLLGLDNLVANTSYLRAQNLDRSELRKLRLTLSLPKPKKSSELELAVGGKYVSLCEQQPIGRKLFQQFLQSSNLQYVAAAEFLEELSHWSFAEDETKEEAKRSILAKFCQPESKSFLSYLTGEEAQRCKALSDKNFNEETINQIKEATRDFLRGKPFSEYLKSPFFYKFLQWKEYEKQKITRRYFYEFRTLGKGGFGEVCAVQVKHTGQMYACKKLDKRRLKKKCGERLALLEKQVLEKVDSLFIVNLAYAFDTKTHLCLVMDLMNGGDLRFHIYELGERGIRMDRVVYYTAQITTGILHLHSLDIVYRDMKPENVLLDAKGQCRLSDLGLAVELPKGKTICQMAGTTGYMAPEILRQDNYNSSVDWWALGCSIYEMVAARLPFKDFREKVQNEEVTRRTLEEECKFEHKRFDAPTKDIISRFLRKKAQHRLGSRGDDPRKNPFFKSINFLRLEAGLVEPPWVPKSNVVYAMNMNTFRDSTEVENIKFNDKDEKFFKEFSTGAVSVKWQKEMIDSGLFDELNDPQQNGHSCETVETSRACIIL

Target ORF information:

RefSeq Version	XM_010737439.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea rhodopsin kinase 2-like (LOC104924175), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010737439.2

ORF Insert Sequence:

ATGTCAAAGA AAACATTCAA TACGAGTGAC CTGTTGGGAT TGGACAACCT GGTGGCCAAC 
ACGTCCTACC TGAGGGCTCA GAATTTGGAT CGCAGTGAGC TGAGGAAGCT GAGACTCACC 
CTGTCGCTGC CCAAACCAAA GAAATCCTCT GAACTAGAGT TGGCAGTGGG GGGGAAGTAC 
GTGTCACTGT GTGAGCAGCA GCCTATTGGG AGGAAGTTGT TCCAGCAGTT TCTCCAGTCC 
TCTAACCTGC AGTATGTGGC CGCTGCTGAG TTCCTGGAGG AGCTGAGTCA CTGGAGCTTT 
GCTGAAGATG AAACTAAAGA GGAGGCCAAA CGGAGCATCC TCGCTAAGTT CTGCCAACCT 
GAATCCAAAA GTTTCCTCTC CTATCTCACA GGAGAGGAAG CTCAAAGATG CAAGGCGTTA 
TCAGACAAGA ACTTTAATGA GGAGACAATA AACCAGATAA AAGAAGCCAC GAGGGACTTC 
CTGAGAGGGA AACCTTTCTC GGAGTATCTG AAAAGCCCCT TTTTTTATAA GTTCCTGCAG 
TGGAAGGAGT ACGAGAAGCA GAAGATCACT AGGAGATACT TTTATGAGTT TAGGACATTG 
GGAAAAGGGG GCTTTGGCGA GGTGTGTGCA GTGCAGGTGA AACACACAGG CCAAATGTAT 
GCCTGCAAAA AGCTGGATAA GAGACGCTTG AAGAAGAAAT GTGGCGAGAG GTTAGCTCTT 
CTGGAGAAGC AGGTCCTGGA GAAGGTCGAC AGCCTCTTTA TCGTAAACTT GGCCTATGCT 
TTCGACACCA AGACCCACCT GTGCCTAGTC ATGGACCTCA TGAACGGAGG AGACCTCAGG 
TTCCATATTT ACGAGCTCGG GGAGCGGGGT ATCCGTATGG ATCGTGTTGT TTACTACACG 
GCACAGATAA CCACTGGGAT ACTCCACCTG CACTCCCTGG ACATTGTGTA CCGTGATATG 
AAGCCTGAGA ACGTGCTCCT GGACGCTAAA GGGCAGTGTC GGCTGTCAGA CCTCGGGTTG 
GCTGTGGAGC TGCCAAAAGG CAAAACAATC TGCCAGATGG CTGGTACGAC AGGTTATATG 
GCTCCTGAGA TTCTGAGGCA GGACAACTAC AACTCATCTG TGGACTGGTG GGCTCTGGGC 
TGCAGCATCT ATGAGATGGT GGCCGCTCGT TTGCCTTTCA AAGACTTCAG AGAGAAAGTG 
CAGAACGAGG AGGTGACTCG CCGCACTCTG GAGGAGGAGT GCAAGTTTGA ACACAAACGC 
TTTGATGCTC CCACCAAAGA TATCATCAGC CGTTTTCTCA GGAAGAAGGC ACAGCATCGC 
CTTGGGAGCC GGGGTGATGA TCCACGAAAA AACCCATTTT TCAAAAGCAT CAATTTCCTT 
CGTCTGGAGG CCGGACTGGT GGAGCCCCCC TGGGTGCCAA AATCCAACGT GGTCTACGCC 
ATGAACATGA ACACGTTCAG GGACAGCACT GAGGTGGAAA ACATTAAGTT CAATGACAAG 
GATGAGAAAT TCTTCAAGGA GTTCAGCACA GGTGCCGTCT CCGTGAAGTG GCAGAAGGAG 
ATGATTGACA GTGGACTCTT TGACGAACTG AACGACCCCC AACAGAACGG CCACAGCTGT 
GAGACTGTGG AGACATCCAG GGCGTGCATC ATTTTGTAA