man2a2 cDNA ORF clone, Labrus bergylta(ballan wrasse)

Gene
Clones
gRNAs

Summary

Gene Symbol	man2a2
Entrez Gene ID	109989707
Full Name	mannosidase alpha class 2A member 2
Gene Type	protein-coding
Organism	Labrus bergylta(ballan wrasse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020641570.1	XP_020497226.1	alpha-mannosidase 2x
XM_020641570.2	XP_020497226.1	alpha-mannosidase 2x

Pathways from BioSystems
Gene Ontology

The following man2a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the man2a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OLa120450	XM_020641570.2 Latest version!	Labrus bergylta mannosidase alpha class 2A member 2 (man2a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OLa120450	XM_020641570.1	Labrus bergylta mannosidase alpha class 2A member 2 (man2a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

man2a2 ( XM_020641570.1 ) cDNA ORF clone, Labrus bergylta(ballan wrasse) -> XP_020497226.1 Labrus bergylta mannosidase alpha class 2A member 2 (man2a2), mRNA.

Price & Availability↑

CloneID	OLa120450
Clone ID Related Accession (Same CDS sequence)	XM_020641570.1 , XM_020641570.2
Accession Version	XM_020641570.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-03-26
Organism	Labrus bergylta(ballan wrasse)
Product	alpha-mannosidase 2x
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018114421.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Labrus bergylta Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA GGAAACAGGT GACAGTTTGT GGTGGGGCCA TATTCTGTGT GGCTGTGTTC 
TCACTGTATC TGATGTTGGA TCGAGTCCAA CATGACCCTG CAAGGCGACA GACCGGCGGG 
AACTTTCCAC GGAGCCAAAT CTCAGTTTTG CAGAACCGGA TAGAGCAGCT GGAGCAGCTC 
CTGGAGGAAA ACCACCAAAT CATCAGCCAC ATAAAGGACT CTGTGATGGA GCTCACAGAC 
ACAGGAGCTG TGTCTCCTAG TGGCCACCTG CCTTTCAGAA GTGCCAATGG TTCCTGGGTC 
CTTCCCTTCG ACGGCCGCCC CACTTTCCTG GCGGTTAAGC CACAAGATTG CCAATTTGCT 
GCAGGCAGCC GTGGTCAAAC GGATGTTCAG ATGCTGGATG TGTACTCCAT CCTGAAGTTT 
GACAACCCTG ATGGCGGTGT ATGGAAACAG GGCTTTGACA TTACTTATGA ACCTGAAGAG 
TGGGACAATG AACCGCTGCA AGTGTTTGTG GTCCCCCACT CACACAATGA TCCAGGTTGG 
ATCAAGACTT TTGACAAGTA CTTCACGGAC CAGACGCAAC ACATTTTAAA CAACATGGTG 
GTGAAGCTGG CGGAGGATCC CCGCAGGAAG TTCATCTGGT CTGAGATTTC TTTCTTTGCC 
AAGTGGTGGG AGGGCGCAGA CATATACAAG CAGGAGGCGA TGCGCAAACT GATCCTTGGA 
GGGCAGCTGG AGATTGTGAC AGGCGGATGG GTGATGACGG ACGAAGCAAA CGTTCACTAC 
TTTGCCATGA TAGACCAGCT CATTGAAGGG CATCAGTGGC TGGAGAGAAA TCTAGGTATT 
ACTCCCCGCA GCGGGTGGGC GGTCGACCCT TTCGGCCACA GCGCTACGAT GCCCTACCTG 
CTGAAGAAAG CCAACCTGAC CAGCATGCTC ATCCAGCGGG TCCACTACTC CATCAAAAAA 
CACTTTGCCT CCACCCGCAG CCTGGAGTTT ATGTGGAGGC AGGCCTGGGA CACTGGATCC 
AGCACGGACA TGTTTTGCCA CATGATGCCT TTCTACAGCT ACGACGTTCC TCACACCTGC 
GGGCCCGACC CCAAGGTCTG CTGCCAGTTT GATTTCAAGA GGTTACCAGG TGGTCGCATT 
AACTGTCCCT GGAAGGTGCC GCCAAAACCT GTGGTGGAGG CCAATGTAGC AGAGAGGGCA 
AACCTCCTCC TAGATCAGTA TCGTAAAAAG TCCAAACTCT ACCGCAGCAA GGTGGTCCTC 
ATCCCCCTGG GAGATGATTT CCGCTACGAC AAGGCCCTGG AATGGGATCA GCAGTACAGC 
AACTACCAGA AACTGTTTGA CTACATGAAT TCTCACCCCG AGATGCACGT TCAAGCCCAG 
TTTGGGACTC TCACCGACTA CTTCAGTGCT GTATACAAAG AACACGGAGT GCCCCAAGGG 
TCCAGACCTG CAGACTTCCC GGTGCTCAGT GGAGATTTTT TTGCTTATGC CGACCGCGAA 
GACCACTACT GGACCGGCTA TTTCACCTCC CGGCCTTTTT ACAAGAGCCT GGACCGTGTT 
ATAGAGTCGC ACCTCAGGGG GGCAGAGATC CTCTACAGCC TAGCAGTTGC GAATGCTCGT 
CATGCTGGGA TGGAAGGGCG GTACCCGGTC TCAGATTATG CCCTGCTGGT CGATGCGAGG 
CGGACTGTTG GCCTCTTTCA ACATCATGAT GCCATCACTG GCACTGCCAA GGAAAATGTT 
GTTGTCGACT ACGGTACTAG ATTACTGCGT TCGCTTATCG GTCTGAAGAG AGTCATTATC 
AACGCTGCTC ATTTCCTGGT GATGAAGAAC AAAGAGTTTT ATCGCTTTTA CCAGACAGAG 
CCATTCCTAG AGACGGATGA CAGGCGGGCT ACCCAAGACT CTCTGCCTCA GCGCACTTTA 
ATCGAGCTGG ACCCTGCAGG GCCGAGGTAT CTGGTTCTGT TCAACCCCAT TGAGCAGGAG 
CGGCTGTGTG CTGTGACTGT GTTGGTCAAT ACGGTCAGGG TGCGGGTGCT CACTGAGGAC 
GGACAGACCC TGCCAGTGCA GCTGAGCGCT CAGTGGAGCT CTGCCAGTCA GATGAGTGCA 
GAGGTATTCG AGGCAACATT CATGGTTCGC CTGCCCCCCC TTGGTCTAGC TGTCTTCCAC 
GTTTATGACG CTCCTGACTC GCCCATGACG CTCCGCTCCG ACACCCTGCT GAGGCTGTCG 
GGTCGCGGCA TCACTGCTCG TGCCGCTGAT TCACTCCCCA TCCGCTCCCA GCAGTCCGAC 
CCTCAGACCT TCTACATCAG CAGTCAGACT CTCACTCTGG GCTTCTCCGG ATCCACCGGC 
CTGCTGGAGA GTATCAAGCG TAAGGATGAT CCTCAGGAAG TGAAGGTTCA GATGCAGTTC 
ATGGTTTACG GCACTCGCCC CTCCAAAGAC AAAAGTGGAG CGTACCTCTT TCTCCCTGAC 
GGGAAAGCGA AGCCCTACAA CCAGAAGGAG CCTCCCGTGG TGCGCGTGGT GGAGGGGCCT 
CTCTTCTCGG AGGTGGTGGC ACACTACCAG CACTTTCAGC AGACCATTCG CATTAACAAC 
GTGCCAGGTG TGGATGGGTT GTCGATAGAC ATCAGCACCG CGGTGGACAT CAGAGATCAG 
ACCAATAAGG AGCTGGCTAT GAGGCTAGAA ACAGACATCC AGAGTGGAGG CGTCTTTTAC 
ACGGATCTCA ACGGCTTCCA GATGCAGCCT CGTCAACACC ACCTCAAGCT CCCCCTGCAG 
GCCAACTTCT ATCCAATGCC CAGCCAGGCG TACATCCAGG ACAGCCATCA CCGCCTCACG 
CTGCACACTG CTCAGTCTCT TGGCGTCAGC AGCCTCGAGA ATGGCCAGCT TGAAGTGATT 
ATGGACCGAC GGCTGATGCA GGATGATAAC CGTGGGCTGG GTCAGGGCCT GAAGGACAAC 
AAGAAGACGG CGAACCGCTT CCGCCTGCTG CTGGAGAGGA GATCCACGGG CAACAAGATG 
GAAGACAGTG CAACAACAAG CTTCCCGTCT ATACTCAGTC ACATGACCAA CGCCTTCCTG 
AACCACGAGG TCCTCGCGCT GCCCGTCCTG CCAAAAAGAC GCGGGATCCC TCCTCTGCAA 
ACCTTCGCCC CTCTCAAGTC CCTTCTGCCC TGCGACTTCC ACATGCTGAA CCTGCGCAGC 
ATCCAGAGCC AGAAGGACCC CCACTCTCCG TCTCCGTACA CAGCTATGAT TCTTCACCGT 
CTGGCGATGG ACTGCGGCGT CGAGGCTCAA AACCTCGGCT TCAACTGCAC CACCACACAA 
GGAGAGCTGA GTGTATCAGG ACTCTTTAAG AACCTGGACC TGCAGCTGCT CCAGCCTGTC 
TCCCTGACTC TGATGCACTC CAGCACGCCT CTGGCCAACG ACTCCTCCAT CAGCCTTGAT 
CCCATGGAGA TCTCTGCCTT TAAGCTCAAG CTGCGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020497226.1
CDS	922..4380
Translation	MKLRKQVTVCGGAIFCVAVFSLYLMLDRVQHDPARRQTGGNFPRSQISVLQNRIEQLEQLLEENHQIISHIKDSVMELTDTGAVSPSGHLPFRSANGSWVLPFDGRPTFLAVKPQDCQFAAGSRGQTDVQMLDVYSILKFDNPDGGVWKQGFDITYEPEEWDNEPLQVFVVPHSHNDPGWIKTFDKYFTDQTQHILNNMVVKLAEDPRRKFIWSEISFFAKWWEGADIYKQEAMRKLILGGQLEIVTGGWVMTDEANVHYFAMIDQLIEGHQWLERNLGITPRSGWAVDPFGHSATMPYLLKKANLTSMLIQRVHYSIKKHFASTRSLEFMWRQAWDTGSSTDMFCHMMPFYSYDVPHTCGPDPKVCCQFDFKRLPGGRINCPWKVPPKPVVEANVAERANLLLDQYRKKSKLYRSKVVLIPLGDDFRYDKALEWDQQYSNYQKLFDYMNSHPEMHVQAQFGTLTDYFSAVYKEHGVPQGSRPADFPVLSGDFFAYADREDHYWTGYFTSRPFYKSLDRVIESHLRGAEILYSLAVANARHAGMEGRYPVSDYALLVDARRTVGLFQHHDAITGTAKENVVVDYGTRLLRSLIGLKRVIINAAHFLVMKNKEFYRFYQTEPFLETDDRRATQDSLPQRTLIELDPAGPRYLVLFNPIEQERLCAVTVLVNTVRVRVLTEDGQTLPVQLSAQWSSASQMSAEVFEATFMVRLPPLGLAVFHVYDAPDSPMTLRSDTLLRLSGRGITARAADSLPIRSQQSDPQTFYISSQTLTLGFSGSTGLLESIKRKDDPQEVKVQMQFMVYGTRPSKDKSGAYLFLPDGKAKPYNQKEPPVVRVVEGPLFSEVVAHYQHFQQTIRINNVPGVDGLSIDISTAVDIRDQTNKELAMRLETDIQSGGVFYTDLNGFQMQPRQHHLKLPLQANFYPMPSQAYIQDSHHRLTLHTAQSLGVSSLENGQLEVIMDRRLMQDDNRGLGQGLKDNKKTANRFRLLLERRSTGNKMEDSATTSFPSILSHMTNAFLNHEVLALPVLPKRRGIPPLQTFAPLKSLLPCDFHMLNLRSIQSQKDPHSPSPYTAMILHRLAMDCGVEAQNLGFNCTTTQGELSVSGLFKNLDLQLLQPVSLTLMHSSTPLANDSSISLDPMEISAFKLKLR

Target ORF information:

RefSeq Version	XM_020641570.1
Organism	Labrus bergylta(ballan wrasse)
Definition	Labrus bergylta mannosidase alpha class 2A member 2 (man2a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020641570.1

ORF Insert Sequence:

ATGAAGCTGA GGAAACAGGT GACAGTTTGT GGTGGGGCCA TATTCTGTGT GGCTGTGTTC 
TCACTGTATC TGATGTTGGA TCGAGTCCAA CATGACCCTG CAAGGCGACA GACCGGCGGG 
AACTTTCCAC GGAGCCAAAT CTCAGTTTTG CAGAACCGGA TAGAGCAGCT GGAGCAGCTC 
CTGGAGGAAA ACCACCAAAT CATCAGCCAC ATAAAGGACT CTGTGATGGA GCTCACAGAC 
ACAGGAGCTG TGTCTCCTAG TGGCCACCTG CCTTTCAGAA GTGCCAATGG TTCCTGGGTC 
CTTCCCTTCG ACGGCCGCCC CACTTTCCTG GCGGTTAAGC CACAAGATTG CCAATTTGCT 
GCAGGCAGCC GTGGTCAAAC GGATGTTCAG ATGCTGGATG TGTACTCCAT CCTGAAGTTT 
GACAACCCTG ATGGCGGTGT ATGGAAACAG GGCTTTGACA TTACTTATGA ACCTGAAGAG 
TGGGACAATG AACCGCTGCA AGTGTTTGTG GTCCCCCACT CACACAATGA TCCAGGTTGG 
ATCAAGACTT TTGACAAGTA CTTCACGGAC CAGACGCAAC ACATTTTAAA CAACATGGTG 
GTGAAGCTGG CGGAGGATCC CCGCAGGAAG TTCATCTGGT CTGAGATTTC TTTCTTTGCC 
AAGTGGTGGG AGGGCGCAGA CATATACAAG CAGGAGGCGA TGCGCAAACT GATCCTTGGA 
GGGCAGCTGG AGATTGTGAC AGGCGGATGG GTGATGACGG ACGAAGCAAA CGTTCACTAC 
TTTGCCATGA TAGACCAGCT CATTGAAGGG CATCAGTGGC TGGAGAGAAA TCTAGGTATT 
ACTCCCCGCA GCGGGTGGGC GGTCGACCCT TTCGGCCACA GCGCTACGAT GCCCTACCTG 
CTGAAGAAAG CCAACCTGAC CAGCATGCTC ATCCAGCGGG TCCACTACTC CATCAAAAAA 
CACTTTGCCT CCACCCGCAG CCTGGAGTTT ATGTGGAGGC AGGCCTGGGA CACTGGATCC 
AGCACGGACA TGTTTTGCCA CATGATGCCT TTCTACAGCT ACGACGTTCC TCACACCTGC 
GGGCCCGACC CCAAGGTCTG CTGCCAGTTT GATTTCAAGA GGTTACCAGG TGGTCGCATT 
AACTGTCCCT GGAAGGTGCC GCCAAAACCT GTGGTGGAGG CCAATGTAGC AGAGAGGGCA 
AACCTCCTCC TAGATCAGTA TCGTAAAAAG TCCAAACTCT ACCGCAGCAA GGTGGTCCTC 
ATCCCCCTGG GAGATGATTT CCGCTACGAC AAGGCCCTGG AATGGGATCA GCAGTACAGC 
AACTACCAGA AACTGTTTGA CTACATGAAT TCTCACCCCG AGATGCACGT TCAAGCCCAG 
TTTGGGACTC TCACCGACTA CTTCAGTGCT GTATACAAAG AACACGGAGT GCCCCAAGGG 
TCCAGACCTG CAGACTTCCC GGTGCTCAGT GGAGATTTTT TTGCTTATGC CGACCGCGAA 
GACCACTACT GGACCGGCTA TTTCACCTCC CGGCCTTTTT ACAAGAGCCT GGACCGTGTT 
ATAGAGTCGC ACCTCAGGGG GGCAGAGATC CTCTACAGCC TAGCAGTTGC GAATGCTCGT 
CATGCTGGGA TGGAAGGGCG GTACCCGGTC TCAGATTATG CCCTGCTGGT CGATGCGAGG 
CGGACTGTTG GCCTCTTTCA ACATCATGAT GCCATCACTG GCACTGCCAA GGAAAATGTT 
GTTGTCGACT ACGGTACTAG ATTACTGCGT TCGCTTATCG GTCTGAAGAG AGTCATTATC 
AACGCTGCTC ATTTCCTGGT GATGAAGAAC AAAGAGTTTT ATCGCTTTTA CCAGACAGAG 
CCATTCCTAG AGACGGATGA CAGGCGGGCT ACCCAAGACT CTCTGCCTCA GCGCACTTTA 
ATCGAGCTGG ACCCTGCAGG GCCGAGGTAT CTGGTTCTGT TCAACCCCAT TGAGCAGGAG 
CGGCTGTGTG CTGTGACTGT GTTGGTCAAT ACGGTCAGGG TGCGGGTGCT CACTGAGGAC 
GGACAGACCC TGCCAGTGCA GCTGAGCGCT CAGTGGAGCT CTGCCAGTCA GATGAGTGCA 
GAGGTATTCG AGGCAACATT CATGGTTCGC CTGCCCCCCC TTGGTCTAGC TGTCTTCCAC 
GTTTATGACG CTCCTGACTC GCCCATGACG CTCCGCTCCG ACACCCTGCT GAGGCTGTCG 
GGTCGCGGCA TCACTGCTCG TGCCGCTGAT TCACTCCCCA TCCGCTCCCA GCAGTCCGAC 
CCTCAGACCT TCTACATCAG CAGTCAGACT CTCACTCTGG GCTTCTCCGG ATCCACCGGC 
CTGCTGGAGA GTATCAAGCG TAAGGATGAT CCTCAGGAAG TGAAGGTTCA GATGCAGTTC 
ATGGTTTACG GCACTCGCCC CTCCAAAGAC AAAAGTGGAG CGTACCTCTT TCTCCCTGAC 
GGGAAAGCGA AGCCCTACAA CCAGAAGGAG CCTCCCGTGG TGCGCGTGGT GGAGGGGCCT 
CTCTTCTCGG AGGTGGTGGC ACACTACCAG CACTTTCAGC AGACCATTCG CATTAACAAC 
GTGCCAGGTG TGGATGGGTT GTCGATAGAC ATCAGCACCG CGGTGGACAT CAGAGATCAG 
ACCAATAAGG AGCTGGCTAT GAGGCTAGAA ACAGACATCC AGAGTGGAGG CGTCTTTTAC 
ACGGATCTCA ACGGCTTCCA GATGCAGCCT CGTCAACACC ACCTCAAGCT CCCCCTGCAG 
GCCAACTTCT ATCCAATGCC CAGCCAGGCG TACATCCAGG ACAGCCATCA CCGCCTCACG 
CTGCACACTG CTCAGTCTCT TGGCGTCAGC AGCCTCGAGA ATGGCCAGCT TGAAGTGATT 
ATGGACCGAC GGCTGATGCA GGATGATAAC CGTGGGCTGG GTCAGGGCCT GAAGGACAAC 
AAGAAGACGG CGAACCGCTT CCGCCTGCTG CTGGAGAGGA GATCCACGGG CAACAAGATG 
GAAGACAGTG CAACAACAAG CTTCCCGTCT ATACTCAGTC ACATGACCAA CGCCTTCCTG 
AACCACGAGG TCCTCGCGCT GCCCGTCCTG CCAAAAAGAC GCGGGATCCC TCCTCTGCAA 
ACCTTCGCCC CTCTCAAGTC CCTTCTGCCC TGCGACTTCC ACATGCTGAA CCTGCGCAGC 
ATCCAGAGCC AGAAGGACCC CCACTCTCCG TCTCCGTACA CAGCTATGAT TCTTCACCGT 
CTGGCGATGG ACTGCGGCGT CGAGGCTCAA AACCTCGGCT TCAACTGCAC CACCACACAA 
GGAGAGCTGA GTGTATCAGG ACTCTTTAAG AACCTGGACC TGCAGCTGCT CCAGCCTGTC 
TCCCTGACTC TGATGCACTC CAGCACGCCT CTGGCCAACG ACTCCTCCAT CAGCCTTGAT 
CCCATGGAGA TCTCTGCCTT TAAGCTCAAG CTGCGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

man2a2 ( XM_020641570.2 ) cDNA ORF clone, Labrus bergylta(ballan wrasse) -> XP_020497226.1 Labrus bergylta mannosidase alpha class 2A member 2 (man2a2), mRNA.

Price & Availability↑

CloneID	OLa120450
Clone ID Related Accession (Same CDS sequence)	XM_020641570.1 , XM_020641570.2
Accession Version	XM_020641570.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-22
Organism	Labrus bergylta(ballan wrasse)
Product	alpha-mannosidase 2x
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018114421.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 23, 2019 this sequence version replaced XM_020641570.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Labrus bergylta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA GGAAACAGGT GACAGTTTGT GGTGGGGCCA TATTCTGTGT GGCTGTGTTC 
TCACTGTATC TGATGTTGGA TCGAGTCCAA CATGACCCTG CAAGGCGACA GACCGGCGGG 
AACTTTCCAC GGAGCCAAAT CTCAGTTTTG CAGAACCGGA TAGAGCAGCT GGAGCAGCTC 
CTGGAGGAAA ACCACCAAAT CATCAGCCAC ATAAAGGACT CTGTGATGGA GCTCACAGAC 
ACAGGAGCTG TGTCTCCTAG TGGCCACCTG CCTTTCAGAA GTGCCAATGG TTCCTGGGTC 
CTTCCCTTCG ACGGCCGCCC CACTTTCCTG GCGGTTAAGC CACAAGATTG CCAATTTGCT 
GCAGGCAGCC GTGGTCAAAC GGATGTTCAG ATGCTGGATG TGTACTCCAT CCTGAAGTTT 
GACAACCCTG ATGGCGGTGT ATGGAAACAG GGCTTTGACA TTACTTATGA ACCTGAAGAG 
TGGGACAATG AACCGCTGCA AGTGTTTGTG GTCCCCCACT CACACAATGA TCCAGGTTGG 
ATCAAGACTT TTGACAAGTA CTTCACGGAC CAGACGCAAC ACATTTTAAA CAACATGGTG 
GTGAAGCTGG CGGAGGATCC CCGCAGGAAG TTCATCTGGT CTGAGATTTC TTTCTTTGCC 
AAGTGGTGGG AGGGCGCAGA CATATACAAG CAGGAGGCGA TGCGCAAACT GATCCTTGGA 
GGGCAGCTGG AGATTGTGAC AGGCGGATGG GTGATGACGG ACGAAGCAAA CGTTCACTAC 
TTTGCCATGA TAGACCAGCT CATTGAAGGG CATCAGTGGC TGGAGAGAAA TCTAGGTATT 
ACTCCCCGCA GCGGGTGGGC GGTCGACCCT TTCGGCCACA GCGCTACGAT GCCCTACCTG 
CTGAAGAAAG CCAACCTGAC CAGCATGCTC ATCCAGCGGG TCCACTACTC CATCAAAAAA 
CACTTTGCCT CCACCCGCAG CCTGGAGTTT ATGTGGAGGC AGGCCTGGGA CACTGGATCC 
AGCACGGACA TGTTTTGCCA CATGATGCCT TTCTACAGCT ACGACGTTCC TCACACCTGC 
GGGCCCGACC CCAAGGTCTG CTGCCAGTTT GATTTCAAGA GGTTACCAGG TGGTCGCATT 
AACTGTCCCT GGAAGGTGCC GCCAAAACCT GTGGTGGAGG CCAATGTAGC AGAGAGGGCA 
AACCTCCTCC TAGATCAGTA TCGTAAAAAG TCCAAACTCT ACCGCAGCAA GGTGGTCCTC 
ATCCCCCTGG GAGATGATTT CCGCTACGAC AAGGCCCTGG AATGGGATCA GCAGTACAGC 
AACTACCAGA AACTGTTTGA CTACATGAAT TCTCACCCCG AGATGCACGT TCAAGCCCAG 
TTTGGGACTC TCACCGACTA CTTCAGTGCT GTATACAAAG AACACGGAGT GCCCCAAGGG 
TCCAGACCTG CAGACTTCCC GGTGCTCAGT GGAGATTTTT TTGCTTATGC CGACCGCGAA 
GACCACTACT GGACCGGCTA TTTCACCTCC CGGCCTTTTT ACAAGAGCCT GGACCGTGTT 
ATAGAGTCGC ACCTCAGGGG GGCAGAGATC CTCTACAGCC TAGCAGTTGC GAATGCTCGT 
CATGCTGGGA TGGAAGGGCG GTACCCGGTC TCAGATTATG CCCTGCTGGT CGATGCGAGG 
CGGACTGTTG GCCTCTTTCA ACATCATGAT GCCATCACTG GCACTGCCAA GGAAAATGTT 
GTTGTCGACT ACGGTACTAG ATTACTGCGT TCGCTTATCG GTCTGAAGAG AGTCATTATC 
AACGCTGCTC ATTTCCTGGT GATGAAGAAC AAAGAGTTTT ATCGCTTTTA CCAGACAGAG 
CCATTCCTAG AGACGGATGA CAGGCGGGCT ACCCAAGACT CTCTGCCTCA GCGCACTTTA 
ATCGAGCTGG ACCCTGCAGG GCCGAGGTAT CTGGTTCTGT TCAACCCCAT TGAGCAGGAG 
CGGCTGTGTG CTGTGACTGT GTTGGTCAAT ACGGTCAGGG TGCGGGTGCT CACTGAGGAC 
GGACAGACCC TGCCAGTGCA GCTGAGCGCT CAGTGGAGCT CTGCCAGTCA GATGAGTGCA 
GAGGTATTCG AGGCAACATT CATGGTTCGC CTGCCCCCCC TTGGTCTAGC TGTCTTCCAC 
GTTTATGACG CTCCTGACTC GCCCATGACG CTCCGCTCCG ACACCCTGCT GAGGCTGTCG 
GGTCGCGGCA TCACTGCTCG TGCCGCTGAT TCACTCCCCA TCCGCTCCCA GCAGTCCGAC 
CCTCAGACCT TCTACATCAG CAGTCAGACT CTCACTCTGG GCTTCTCCGG ATCCACCGGC 
CTGCTGGAGA GTATCAAGCG TAAGGATGAT CCTCAGGAAG TGAAGGTTCA GATGCAGTTC 
ATGGTTTACG GCACTCGCCC CTCCAAAGAC AAAAGTGGAG CGTACCTCTT TCTCCCTGAC 
GGGAAAGCGA AGCCCTACAA CCAGAAGGAG CCTCCCGTGG TGCGCGTGGT GGAGGGGCCT 
CTCTTCTCGG AGGTGGTGGC ACACTACCAG CACTTTCAGC AGACCATTCG CATTAACAAC 
GTGCCAGGTG TGGATGGGTT GTCGATAGAC ATCAGCACCG CGGTGGACAT CAGAGATCAG 
ACCAATAAGG AGCTGGCTAT GAGGCTAGAA ACAGACATCC AGAGTGGAGG CGTCTTTTAC 
ACGGATCTCA ACGGCTTCCA GATGCAGCCT CGTCAACACC ACCTCAAGCT CCCCCTGCAG 
GCCAACTTCT ATCCAATGCC CAGCCAGGCG TACATCCAGG ACAGCCATCA CCGCCTCACG 
CTGCACACTG CTCAGTCTCT TGGCGTCAGC AGCCTCGAGA ATGGCCAGCT TGAAGTGATT 
ATGGACCGAC GGCTGATGCA GGATGATAAC CGTGGGCTGG GTCAGGGCCT GAAGGACAAC 
AAGAAGACGG CGAACCGCTT CCGCCTGCTG CTGGAGAGGA GATCCACGGG CAACAAGATG 
GAAGACAGTG CAACAACAAG CTTCCCGTCT ATACTCAGTC ACATGACCAA CGCCTTCCTG 
AACCACGAGG TCCTCGCGCT GCCCGTCCTG CCAAAAAGAC GCGGGATCCC TCCTCTGCAA 
ACCTTCGCCC CTCTCAAGTC CCTTCTGCCC TGCGACTTCC ACATGCTGAA CCTGCGCAGC 
ATCCAGAGCC AGAAGGACCC CCACTCTCCG TCTCCGTACA CAGCTATGAT TCTTCACCGT 
CTGGCGATGG ACTGCGGCGT CGAGGCTCAA AACCTCGGCT TCAACTGCAC CACCACACAA 
GGAGAGCTGA GTGTATCAGG ACTCTTTAAG AACCTGGACC TGCAGCTGCT CCAGCCTGTC 
TCCCTGACTC TGATGCACTC CAGCACGCCT CTGGCCAACG ACTCCTCCAT CAGCCTTGAT 
CCCATGGAGA TCTCTGCCTT TAAGCTCAAG CTGCGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020497226.1
CDS	942..4400
Translation	MKLRKQVTVCGGAIFCVAVFSLYLMLDRVQHDPARRQTGGNFPRSQISVLQNRIEQLEQLLEENHQIISHIKDSVMELTDTGAVSPSGHLPFRSANGSWVLPFDGRPTFLAVKPQDCQFAAGSRGQTDVQMLDVYSILKFDNPDGGVWKQGFDITYEPEEWDNEPLQVFVVPHSHNDPGWIKTFDKYFTDQTQHILNNMVVKLAEDPRRKFIWSEISFFAKWWEGADIYKQEAMRKLILGGQLEIVTGGWVMTDEANVHYFAMIDQLIEGHQWLERNLGITPRSGWAVDPFGHSATMPYLLKKANLTSMLIQRVHYSIKKHFASTRSLEFMWRQAWDTGSSTDMFCHMMPFYSYDVPHTCGPDPKVCCQFDFKRLPGGRINCPWKVPPKPVVEANVAERANLLLDQYRKKSKLYRSKVVLIPLGDDFRYDKALEWDQQYSNYQKLFDYMNSHPEMHVQAQFGTLTDYFSAVYKEHGVPQGSRPADFPVLSGDFFAYADREDHYWTGYFTSRPFYKSLDRVIESHLRGAEILYSLAVANARHAGMEGRYPVSDYALLVDARRTVGLFQHHDAITGTAKENVVVDYGTRLLRSLIGLKRVIINAAHFLVMKNKEFYRFYQTEPFLETDDRRATQDSLPQRTLIELDPAGPRYLVLFNPIEQERLCAVTVLVNTVRVRVLTEDGQTLPVQLSAQWSSASQMSAEVFEATFMVRLPPLGLAVFHVYDAPDSPMTLRSDTLLRLSGRGITARAADSLPIRSQQSDPQTFYISSQTLTLGFSGSTGLLESIKRKDDPQEVKVQMQFMVYGTRPSKDKSGAYLFLPDGKAKPYNQKEPPVVRVVEGPLFSEVVAHYQHFQQTIRINNVPGVDGLSIDISTAVDIRDQTNKELAMRLETDIQSGGVFYTDLNGFQMQPRQHHLKLPLQANFYPMPSQAYIQDSHHRLTLHTAQSLGVSSLENGQLEVIMDRRLMQDDNRGLGQGLKDNKKTANRFRLLLERRSTGNKMEDSATTSFPSILSHMTNAFLNHEVLALPVLPKRRGIPPLQTFAPLKSLLPCDFHMLNLRSIQSQKDPHSPSPYTAMILHRLAMDCGVEAQNLGFNCTTTQGELSVSGLFKNLDLQLLQPVSLTLMHSSTPLANDSSISLDPMEISAFKLKLR

Target ORF information:

RefSeq Version	XM_020641570.2
Organism	Labrus bergylta(ballan wrasse)
Definition	Labrus bergylta mannosidase alpha class 2A member 2 (man2a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020641570.2

ORF Insert Sequence:

ATGAAGCTGA GGAAACAGGT GACAGTTTGT GGTGGGGCCA TATTCTGTGT GGCTGTGTTC 
TCACTGTATC TGATGTTGGA TCGAGTCCAA CATGACCCTG CAAGGCGACA GACCGGCGGG 
AACTTTCCAC GGAGCCAAAT CTCAGTTTTG CAGAACCGGA TAGAGCAGCT GGAGCAGCTC 
CTGGAGGAAA ACCACCAAAT CATCAGCCAC ATAAAGGACT CTGTGATGGA GCTCACAGAC 
ACAGGAGCTG TGTCTCCTAG TGGCCACCTG CCTTTCAGAA GTGCCAATGG TTCCTGGGTC 
CTTCCCTTCG ACGGCCGCCC CACTTTCCTG GCGGTTAAGC CACAAGATTG CCAATTTGCT 
GCAGGCAGCC GTGGTCAAAC GGATGTTCAG ATGCTGGATG TGTACTCCAT CCTGAAGTTT 
GACAACCCTG ATGGCGGTGT ATGGAAACAG GGCTTTGACA TTACTTATGA ACCTGAAGAG 
TGGGACAATG AACCGCTGCA AGTGTTTGTG GTCCCCCACT CACACAATGA TCCAGGTTGG 
ATCAAGACTT TTGACAAGTA CTTCACGGAC CAGACGCAAC ACATTTTAAA CAACATGGTG 
GTGAAGCTGG CGGAGGATCC CCGCAGGAAG TTCATCTGGT CTGAGATTTC TTTCTTTGCC 
AAGTGGTGGG AGGGCGCAGA CATATACAAG CAGGAGGCGA TGCGCAAACT GATCCTTGGA 
GGGCAGCTGG AGATTGTGAC AGGCGGATGG GTGATGACGG ACGAAGCAAA CGTTCACTAC 
TTTGCCATGA TAGACCAGCT CATTGAAGGG CATCAGTGGC TGGAGAGAAA TCTAGGTATT 
ACTCCCCGCA GCGGGTGGGC GGTCGACCCT TTCGGCCACA GCGCTACGAT GCCCTACCTG 
CTGAAGAAAG CCAACCTGAC CAGCATGCTC ATCCAGCGGG TCCACTACTC CATCAAAAAA 
CACTTTGCCT CCACCCGCAG CCTGGAGTTT ATGTGGAGGC AGGCCTGGGA CACTGGATCC 
AGCACGGACA TGTTTTGCCA CATGATGCCT TTCTACAGCT ACGACGTTCC TCACACCTGC 
GGGCCCGACC CCAAGGTCTG CTGCCAGTTT GATTTCAAGA GGTTACCAGG TGGTCGCATT 
AACTGTCCCT GGAAGGTGCC GCCAAAACCT GTGGTGGAGG CCAATGTAGC AGAGAGGGCA 
AACCTCCTCC TAGATCAGTA TCGTAAAAAG TCCAAACTCT ACCGCAGCAA GGTGGTCCTC 
ATCCCCCTGG GAGATGATTT CCGCTACGAC AAGGCCCTGG AATGGGATCA GCAGTACAGC 
AACTACCAGA AACTGTTTGA CTACATGAAT TCTCACCCCG AGATGCACGT TCAAGCCCAG 
TTTGGGACTC TCACCGACTA CTTCAGTGCT GTATACAAAG AACACGGAGT GCCCCAAGGG 
TCCAGACCTG CAGACTTCCC GGTGCTCAGT GGAGATTTTT TTGCTTATGC CGACCGCGAA 
GACCACTACT GGACCGGCTA TTTCACCTCC CGGCCTTTTT ACAAGAGCCT GGACCGTGTT 
ATAGAGTCGC ACCTCAGGGG GGCAGAGATC CTCTACAGCC TAGCAGTTGC GAATGCTCGT 
CATGCTGGGA TGGAAGGGCG GTACCCGGTC TCAGATTATG CCCTGCTGGT CGATGCGAGG 
CGGACTGTTG GCCTCTTTCA ACATCATGAT GCCATCACTG GCACTGCCAA GGAAAATGTT 
GTTGTCGACT ACGGTACTAG ATTACTGCGT TCGCTTATCG GTCTGAAGAG AGTCATTATC 
AACGCTGCTC ATTTCCTGGT GATGAAGAAC AAAGAGTTTT ATCGCTTTTA CCAGACAGAG 
CCATTCCTAG AGACGGATGA CAGGCGGGCT ACCCAAGACT CTCTGCCTCA GCGCACTTTA 
ATCGAGCTGG ACCCTGCAGG GCCGAGGTAT CTGGTTCTGT TCAACCCCAT TGAGCAGGAG 
CGGCTGTGTG CTGTGACTGT GTTGGTCAAT ACGGTCAGGG TGCGGGTGCT CACTGAGGAC 
GGACAGACCC TGCCAGTGCA GCTGAGCGCT CAGTGGAGCT CTGCCAGTCA GATGAGTGCA 
GAGGTATTCG AGGCAACATT CATGGTTCGC CTGCCCCCCC TTGGTCTAGC TGTCTTCCAC 
GTTTATGACG CTCCTGACTC GCCCATGACG CTCCGCTCCG ACACCCTGCT GAGGCTGTCG 
GGTCGCGGCA TCACTGCTCG TGCCGCTGAT TCACTCCCCA TCCGCTCCCA GCAGTCCGAC 
CCTCAGACCT TCTACATCAG CAGTCAGACT CTCACTCTGG GCTTCTCCGG ATCCACCGGC 
CTGCTGGAGA GTATCAAGCG TAAGGATGAT CCTCAGGAAG TGAAGGTTCA GATGCAGTTC 
ATGGTTTACG GCACTCGCCC CTCCAAAGAC AAAAGTGGAG CGTACCTCTT TCTCCCTGAC 
GGGAAAGCGA AGCCCTACAA CCAGAAGGAG CCTCCCGTGG TGCGCGTGGT GGAGGGGCCT 
CTCTTCTCGG AGGTGGTGGC ACACTACCAG CACTTTCAGC AGACCATTCG CATTAACAAC 
GTGCCAGGTG TGGATGGGTT GTCGATAGAC ATCAGCACCG CGGTGGACAT CAGAGATCAG 
ACCAATAAGG AGCTGGCTAT GAGGCTAGAA ACAGACATCC AGAGTGGAGG CGTCTTTTAC 
ACGGATCTCA ACGGCTTCCA GATGCAGCCT CGTCAACACC ACCTCAAGCT CCCCCTGCAG 
GCCAACTTCT ATCCAATGCC CAGCCAGGCG TACATCCAGG ACAGCCATCA CCGCCTCACG 
CTGCACACTG CTCAGTCTCT TGGCGTCAGC AGCCTCGAGA ATGGCCAGCT TGAAGTGATT 
ATGGACCGAC GGCTGATGCA GGATGATAAC CGTGGGCTGG GTCAGGGCCT GAAGGACAAC 
AAGAAGACGG CGAACCGCTT CCGCCTGCTG CTGGAGAGGA GATCCACGGG CAACAAGATG 
GAAGACAGTG CAACAACAAG CTTCCCGTCT ATACTCAGTC ACATGACCAA CGCCTTCCTG 
AACCACGAGG TCCTCGCGCT GCCCGTCCTG CCAAAAAGAC GCGGGATCCC TCCTCTGCAA 
ACCTTCGCCC CTCTCAAGTC CCTTCTGCCC TGCGACTTCC ACATGCTGAA CCTGCGCAGC 
ATCCAGAGCC AGAAGGACCC CCACTCTCCG TCTCCGTACA CAGCTATGAT TCTTCACCGT 
CTGGCGATGG ACTGCGGCGT CGAGGCTCAA AACCTCGGCT TCAACTGCAC CACCACACAA 
GGAGAGCTGA GTGTATCAGG ACTCTTTAAG AACCTGGACC TGCAGCTGCT CCAGCCTGTC 
TCCCTGACTC TGATGCACTC CAGCACGCCT CTGGCCAACG ACTCCTCCAT CAGCCTTGAT 
CCCATGGAGA TCTCTGCCTT TAAGCTCAAG CTGCGCTAA