MANF cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MANF
Entrez Gene ID	7873
Full Name	mesencephalic astrocyte derived neurotrophic factor
Synonyms	ARMET,ARP
General protein information	Preferred Names mesencephalic astrocyte derived neurotrophic factor Names mesencephalic astrocyte-derived neurotrophic factor arginine-rich, mutated in early stage tumors
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p21.2
Summary	The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related. [provided by RefSeq, Apr 2014].
Disorder MIM:	601916

mRNA and Protein(s)

mRNA	Protein	Name
NM_006010.6	NP_006001.5	mesencephalic astrocyte-derived neurotrophic factor precursor
NM_006010.5	NP_006001.4	mesencephalic astrocyte-derived neurotrophic factor precursor

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	Y54G2A.23	NP_500273.2
Bos taurus (cattle)	MANF	NP_001094681.1
Drosophila melanogaster (fruit fly)	Manf	NP_477445.1
Homo sapiens (human)	MANF	NP_006001.3
Mus musculus (house mouse)	Manf	NP_083379.2
	LOC700659	XP_002802839.1
Danio rerio (zebrafish)	manf	NP_001070097.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003016	XP_311862.3
Pan troglodytes (chimpanzee)	MANF	XP_001169644.3
Canis lupus familiaris (dog)	MANF	XP_003639808.2
Xenopus tropicalis (tropical clawed frog)	manf	NP_001016425.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of two sequence motifs of mesencephalic astrocyte-derived neurotrophic factor in its survival-promoting activity.
M?tlik K, Yu LY, Eesmaa A, Hellman M, Lindholm P, Per?nen J, Galli E, Anttila J, Saarma M, Permi P, Airavaara M, Arum?e U
Cell death & disease6e2032(2015 Dec)

ER stress-inducible protein MANF selectively expresses in human spleen.
Liu J, Zhou C, Tao X, Feng L, Wang X, Chen L, Li C, Huang D, Fang S, Shen Y
Human immunology76(11)823-30(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Serum MANF level was higher in patients with newly diagnosed prediabetes and T2 Diabetes Mellitus than in normal glucose tolerance controls. MANF appears to be associated with Matsuda Index, QUICKI and HOMA-IR in prediabetes patients.
Title: Circulating mesencephalic astrocyte-derived neurotrophic factor is increased in newly diagnosed prediabetic and diabetic patients, and is associated with insulin resistance.

the role of two short sequence motifs within the carboxy-(C) terminal domain of MANF in its neuroprotective activity, was studied.
Title: Role of two sequence motifs of mesencephalic astrocyte-derived neurotrophic factor in its survival-promoting activity.

the selective expression of MANF in splenocytes may be involved in plasma cell differentiation and immune regulation.
Title: ER stress-inducible protein MANF selectively expresses in human spleen.

MANF is a protein that interacts with RTN1-C
Title: Identification of MANF as a protein interacting with RTN1-C.

We demonstrate that Armet and Creld2 are genotype-specific ER stress response proteins with substrate specificities, and that aggregation of mutant matrilin-3 is a key disease trigger in MED that could be exploited as a potential therapeutic target
Title: Armet/Manf and Creld2 are components of a specialized ER stress response provoked by inappropriate formation of disulphide bonds: implications for genetic skeletal diseases.

The following MANF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MANF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu24009	NM_006010.5 Latest version!	Homo sapiens mesencephalic astrocyte derived neurotrophic factor (MANF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu107623	NM_006010.6 Latest version!	Homo sapiens mesencephalic astrocyte derived neurotrophic factor (MANF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MANF ( NM_006010.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_006001.4 Homo sapiens mesencephalic astrocyte derived neurotrophic factor (MANF), mRNA.

Price & Availability↑

CloneID	OHu24009
Clone ID Related Accession (Same CDS sequence)	NM_006010.5
Accession Version	NM_006010.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	mesencephalic astrocyte-derived neurotrophic factor precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M83751.1 and BC007282.1. This sequence is a reference standard in the RefSeqGene project. On Apr 24, 2014 this sequence version replaced NM_006010.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC007282.1, M83751.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGAGGA TGAGGAGGAT GTGGGCCACG CAGGGGCTGG CGGTGGCGCT GGCTCTGAGC 
GTGCTGCCGG GCAGCCGGGC GCTGCGGCCG GGCGACTGCG AAGTTTGTAT TTCTTATCTG 
GGAAGATTTT ACCAGGACCT CAAAGACAGA GATGTCACAT TCTCACCAGC CACTATTGAA 
AACGAACTTA TAAAGTTCTG CCGGGAAGCA AGAGGCAAAG AGAATCGGTT GTGCTACTAT 
ATCGGGGCCA CAGATGATGC AGCCACCAAA ATCATCAATG AGGTATCAAA GCCTCTGGCC 
CACCACATCC CTGTGGAGAA GATCTGTGAG AAGCTTAAGA AGAAGGACAG CCAGATATGT 
GAGCTTAAGT ATGACAAGCA GATCGACCTG AGCACAGTGG ACCTGAAGAA GCTCCGAGTT 
AAAGAGCTGA AGAAGATTCT GGATGACTGG GGGGAGACAT GCAAAGGCTG TGCAGAAAAG 
TCTGACTACA TCCGGAAGAT AAATGAACTG ATGCCTAAAT ATGCCCCCAA GGCAGCCAGT 
GCACGGACCG ATTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006001.4
CDS	98..655
Translation	MRRMRRMWATQGLAVALALSVLPGSRALRPGDCEVCISYLGRFYQDLKDRDVTFSPATIENELIKFCREARGKENRLCYYIGATDDAATKIINEVSKPLAHHIPVEKICEKLKKKDSQICELKYDKQIDLSTVDLKKLRVKELKKILDDWGETCKGCAEKSDYIRKINELMPKYAPKAASARTDL

Target ORF information:

RefSeq Version	NM_006010.5
Organism	Homo sapiens(human)
Definition	Homo sapiens mesencephalic astrocyte derived neurotrophic factor (MANF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006010.5

ORF Insert Sequence:

ATGAGGAGGA TGAGGAGGAT GTGGGCCACG CAGGGGCTGG CGGTGGCGCT GGCTCTGAGC 
GTGCTGCCGG GCAGCCGGGC GCTGCGGCCG GGCGACTGCG AAGTTTGTAT TTCTTATCTG 
GGAAGATTTT ACCAGGACCT CAAAGACAGA GATGTCACAT TCTCACCAGC CACTATTGAA 
AACGAACTTA TAAAGTTCTG CCGGGAAGCA AGAGGCAAAG AGAATCGGTT GTGCTACTAT 
ATCGGGGCCA CAGATGATGC AGCCACCAAA ATCATCAATG AGGTATCAAA GCCTCTGGCC 
CACCACATCC CTGTGGAGAA GATCTGTGAG AAGCTTAAGA AGAAGGACAG CCAGATATGT 
GAGCTTAAGT ATGACAAGCA GATCGACCTG AGCACAGTGG ACCTGAAGAA GCTCCGAGTT 
AAAGAGCTGA AGAAGATTCT GGATGACTGG GGGGAGACAT GCAAAGGCTG TGCAGAAAAG 
TCTGACTACA TCCGGAAGAT AAATGAACTG ATGCCTAAAT ATGCCCCCAA GGCAGCCAGT 
GCACGGACCG ATTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MANF ( NM_006010.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_006001.5 Homo sapiens mesencephalic astrocyte derived neurotrophic factor (MANF), mRNA.

Price & Availability↑

CloneID	OHu107623
Clone ID Related Accession (Same CDS sequence)	NM_006010.6
Accession Version	NM_006010.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	mesencephalic astrocyte-derived neurotrophic factor precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M83751.1 and BC007282.1. This sequence is a reference standard in the RefSeqGene project. On Sep 20, 2018 this sequence version replaced NM_006010.5. CCDS Note: The coding region has been updated to shorten the N-terminus, using a downstream start codon that is better supported by conservation data, and is supported by ribosome profiling data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC007282.1, SRR7346977.531546.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved MANE Ensembl match :: ENST00000528157.7/ ENSP00000432799.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGGAGGA TGTGGGCCAC GCAGGGGCTG GCGGTGGCGC TGGCTCTGAG CGTGCTGCCG 
GGCAGCCGGG CGCTGCGGCC GGGCGACTGC GAAGTTTGTA TTTCTTATCT GGGAAGATTT 
TACCAGGACC TCAAAGACAG AGATGTCACA TTCTCACCAG CCACTATTGA AAACGAACTT 
ATAAAGTTCT GCCGGGAAGC AAGAGGCAAA GAGAATCGGT TGTGCTACTA TATCGGGGCC 
ACAGATGATG CAGCCACCAA AATCATCAAT GAGGTATCAA AGCCTCTGGC CCACCACATC 
CCTGTGGAGA AGATCTGTGA GAAGCTTAAG AAGAAGGACA GCCAGATATG TGAGCTTAAG 
TATGACAAGC AGATCGACCT GAGCACAGTG GACCTGAAGA AGCTCCGAGT TAAAGAGCTG 
AAGAAGATTC TGGATGACTG GGGGGAGACA TGCAAAGGCT GTGCAGAAAA GTCTGACTAC 
ATCCGGAAGA TAAATGAACT GATGCCTAAA TATGCCCCCA AGGCAGCCAG TGCACGGACC 
GATTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006001.5
CDS	53..601
Translation	MRRMWATQGLAVALALSVLPGSRALRPGDCEVCISYLGRFYQDLKDRDVTFSPATIENELIKFCREARGKENRLCYYIGATDDAATKIINEVSKPLAHHIPVEKICEKLKKKDSQICELKYDKQIDLSTVDLKKLRVKELKKILDDWGETCKGCAEKSDYIRKINELMPKYAPKAASARTDL

Target ORF information:

RefSeq Version	NM_006010.6
Organism	Homo sapiens(human)
Definition	Homo sapiens mesencephalic astrocyte derived neurotrophic factor (MANF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006010.6

ORF Insert Sequence:

ATGAGGAGGA TGTGGGCCAC GCAGGGGCTG GCGGTGGCGC TGGCTCTGAG CGTGCTGCCG 
GGCAGCCGGG CGCTGCGGCC GGGCGACTGC GAAGTTTGTA TTTCTTATCT GGGAAGATTT 
TACCAGGACC TCAAAGACAG AGATGTCACA TTCTCACCAG CCACTATTGA AAACGAACTT 
ATAAAGTTCT GCCGGGAAGC AAGAGGCAAA GAGAATCGGT TGTGCTACTA TATCGGGGCC 
ACAGATGATG CAGCCACCAA AATCATCAAT GAGGTATCAA AGCCTCTGGC CCACCACATC 
CCTGTGGAGA AGATCTGTGA GAAGCTTAAG AAGAAGGACA GCCAGATATG TGAGCTTAAG 
TATGACAAGC AGATCGACCT GAGCACAGTG GACCTGAAGA AGCTCCGAGT TAAAGAGCTG 
AAGAAGATTC TGGATGACTG GGGGGAGACA TGCAAAGGCT GTGCAGAAAA GTCTGACTAC 
ATCCGGAAGA TAAATGAACT GATGCCTAAA TATGCCCCCA AGGCAGCCAG TGCACGGACC 
GATTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of two sequence motifs of mesencephalic astrocyte-derived neurotrophic factor in its survival-promoting activity.
Cell death & disease6e2032(2015 Dec)
M?tlik K,Yu LY,Eesmaa A,Hellman M,Lindholm P,Per?nen J,Galli E,Anttila J,Saarma M,Permi P,Airavaara M,Arum?e U

ER stress-inducible protein MANF selectively expresses in human spleen.
Human immunology76(11)823-30(2015 Nov)
Liu J,Zhou C,Tao X,Feng L,Wang X,Chen L,Li C,Huang D,Fang S,Shen Y

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MANF cDNA ORF clone, Homo sapiens(human)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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