SHISA9 cDNA ORF clone, Homo sapiens(human)

The following SHISA9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SHISA9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu14495 NM_001145204.3
Latest version!
Homo sapiens shisa family member 9 (SHISA9), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu15067 NM_001145205.2
Latest version!
Homo sapiens shisa family member 9 (SHISA9), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu80904 XM_005255539.3
Latest version!
Homo sapiens shisa family member 9 (SHISA9), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $223.30
$319.00
OHu80905 XM_011522642.2
Latest version!
Homo sapiens shisa family member 9 (SHISA9), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $223.30
$319.00
View Old Accession Versions >>
OHu15067 NM_001145205.1 Homo sapiens shisa family member 9 (SHISA9), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu14495 NM_001145204.2 Homo sapiens shisa family member 9 (SHISA9), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu14495
    Clone ID Related Accession (Same CDS sequence) NM_001145204.2 , NM_001145204.3
    Accession Version NM_001145204.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-26
    Organism Homo sapiens(human)
    Product protein shisa-9 isoform 1 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC375222.1, AC092324.3, AK299610.1, AC009134.6 and H20826.1. On Mar 24, 2010 this sequence version replaced NM_001145204.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). This RefSeq contains an in-frame start site 41 codons upstream from the currently annotated site but is not being annotated as a start site since it is in a weak Kozak sequence context. Also, use of the upstream AUG would eliminate the signal peptide. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCGCCGCG TCCTTCGGCT GCTCCTCGGT TGCTTCCTCA CCGAGCTGTG CGCCCGCGTG 
    TGCCGGGCGC AGGAGCGAGC GGGACACGGG CAGCTGGCGC AACTGGGCGG CGTGTTGCTG
    CTGGCGGGGG GCAACCGCTC CGGGGCCGCC TCCGGAGAGG CCAGCGAGGG CGCTGAGGCA
    TCGGACGCGC CCCCGACCCG GGCGCCCACG CCGGACTTCT GCCGGGGCTA CTTCGATGTC
    ATGGGCCAGT GGGACCCGCC GTTCAACTGC AGCTCGGGCG ACTTCATCTT CTGCTGCGGG
    ACTTGTGGCT TCCGGTTCTG CTGCACGTTT AAGAAGCGGC GACTGAACCA AAGCACCTGC
    ACCAACTACG ACACGCCGCT CTGGCTCAAC ACCGGCAAGC CCCCCGCCCG CAAGGACGAC
    CCCTTGCACG ACCCCACCAA GGACAAGACC AACCTGATCG TCTACATCAT CTGCGGGGTG
    GTGGCCGTCA TGGTGCTCGT GGGCATCTTC ACCAAGCTGG GGCTGGAGAA AGCGCACCGG
    CCCCAAAGGG AGCACATGTC CAGGGCCCTT GCGGATGTCA TGAGACCACA GGGCCACTGC
    AACACTGATC ACATGGAGAG AGACCTAAAC ATCGTTGTCC ACGTCCAGCA TTATGAGAAC
    ATGGACACGA GAACCCCCAT AAATAATCTT CATGCCACCC AGATGAACAA CGCAGTGCCC
    ACCTCTCCTC TGCTCCAGCA GATGGGCCAT CCACATTCGT ACCCGAACCT GGGCCAGATC
    TCCAACCCCT ATGAACAGCA GCCACCAGGA AAAGAGCTCA ACAAGTACGC CTCCTTAAAG
    GCAGTCGGAA GTTCTGATGG TGACTGGGCA GTATCGACAC TTAAGTCACC AAAAGCTGAC
    AAGGTCAATG ACGACTTCTA CACCAAGCGA CGGCACCTGG CTGAGCTGGC TGCCAAGGGG
    AACTTACCTC TGCACCCCGT AAGAGTGGAG GACGAGCCCC GGGCCTTCAG CCCTGAGCAC
    GGTCCTGCCA AGCAGAATGG ACAGAAGTCC CGCACCAACA AGATGCCCCC ACATCCCCTG
    GCCTACACCT CTACCACCAA CTTTAAGGGC TGGGACCCCA ACGAGCAGTC CCTCCGGCGG
    CAGGCTTACA GCAACAAGGG CAAGCTTGGC ACGGCCGAGA CAGGCTCCAG CGACCCCTTG
    GGAACTCGCC CCCAGCACTA CCCACCCCCA CAGCCATACT TCATCACCAA CAGCAAAACA
    GAAGTGACTG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138676.2
    CDS446..1720
    Translation

    Target ORF information:

    RefSeq Version NM_001145204.2
    Organism Homo sapiens(human)
    Definition Homo sapiens shisa family member 9 (SHISA9), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001145204.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCGCCGCG TCCTTCGGCT GCTCCTCGGT TGCTTCCTCA CCGAGCTGTG CGCCCGCGTG 
    TGCCGGGCGC AGGAGCGAGC GGGACACGGG CAGCTGGCGC AACTGGGCGG CGTGTTGCTG
    CTGGCGGGGG GCAACCGCTC CGGGGCCGCC TCCGGAGAGG CCAGCGAGGG CGCTGAGGCA
    TCGGACGCGC CCCCGACCCG GGCGCCCACG CCGGACTTCT GCCGGGGCTA CTTCGATGTC
    ATGGGCCAGT GGGACCCGCC GTTCAACTGC AGCTCGGGCG ACTTCATCTT CTGCTGCGGG
    ACTTGTGGCT TCCGGTTCTG CTGCACGTTT AAGAAGCGGC GACTGAACCA AAGCACCTGC
    ACCAACTACG ACACGCCGCT CTGGCTCAAC ACCGGCAAGC CCCCCGCCCG CAAGGACGAC
    CCCTTGCACG ACCCCACCAA GGACAAGACC AACCTGATCG TCTACATCAT CTGCGGGGTG
    GTGGCCGTCA TGGTGCTCGT GGGCATCTTC ACCAAGCTGG GGCTGGAGAA AGCGCACCGG
    CCCCAAAGGG AGCACATGTC CAGGGCCCTT GCGGATGTCA TGAGACCACA GGGCCACTGC
    AACACTGATC ACATGGAGAG AGACCTAAAC ATCGTTGTCC ACGTCCAGCA TTATGAGAAC
    ATGGACACGA GAACCCCCAT AAATAATCTT CATGCCACCC AGATGAACAA CGCAGTGCCC
    ACCTCTCCTC TGCTCCAGCA GATGGGCCAT CCACATTCGT ACCCGAACCT GGGCCAGATC
    TCCAACCCCT ATGAACAGCA GCCACCAGGA AAAGAGCTCA ACAAGTACGC CTCCTTAAAG
    GCAGTCGGAA GTTCTGATGG TGACTGGGCA GTATCGACAC TTAAGTCACC AAAAGCTGAC
    AAGGTCAATG ACGACTTCTA CACCAAGCGA CGGCACCTGG CTGAGCTGGC TGCCAAGGGG
    AACTTACCTC TGCACCCCGT AAGAGTGGAG GACGAGCCCC GGGCCTTCAG CCCTGAGCAC
    GGTCCTGCCA AGCAGAATGG ACAGAAGTCC CGCACCAACA AGATGCCCCC ACATCCCCTG
    GCCTACACCT CTACCACCAA CTTTAAGGGC TGGGACCCCA ACGAGCAGTC CCTCCGGCGG
    CAGGCTTACA GCAACAAGGG CAAGCTTGGC ACGGCCGAGA CAGGCTCCAG CGACCCCTTG
    GGAACTCGCC CCCAGCACTA CCCACCCCCA CAGCCATACT TCATCACCAA CAGCAAAACA
    GAAGTGACTG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14495
    Clone ID Related Accession (Same CDS sequence) NM_001145204.2 , NM_001145204.3
    Accession Version NM_001145204.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-30
    Organism Homo sapiens(human)
    Product protein shisa-9 isoform 1 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092324.3, DC375222.1, AK299610.1, AC009134.6 and H20826.1. On Nov 23, 2018 this sequence version replaced NM_001145204.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). This RefSeq contains an in-frame start site 41 codons upstream from the currently annotated site but is not being annotated as a start site since it is in a weak Kozak sequence context. Also, use of the upstream AUG would eliminate the signal peptide. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal MANE Ensembl match :: ENST00000558583.3/ ENSP00000454014.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCGCCGCG TCCTTCGGCT GCTCCTCGGT TGCTTCCTCA CCGAGCTGTG CGCCCGCGTG 
    TGCCGGGCGC AGGAGCGAGC GGGACACGGG CAGCTGGCGC AACTGGGCGG CGTGTTGCTG
    CTGGCGGGGG GCAACCGCTC CGGGGCCGCC TCCGGAGAGG CCAGCGAGGG CGCTGAGGCA
    TCGGACGCGC CCCCGACCCG GGCGCCCACG CCGGACTTCT GCCGGGGCTA CTTCGATGTC
    ATGGGCCAGT GGGACCCGCC GTTCAACTGC AGCTCGGGCG ACTTCATCTT CTGCTGCGGG
    ACTTGTGGCT TCCGGTTCTG CTGCACGTTT AAGAAGCGGC GACTGAACCA AAGCACCTGC
    ACCAACTACG ACACGCCGCT CTGGCTCAAC ACCGGCAAGC CCCCCGCCCG CAAGGACGAC
    CCCTTGCACG ACCCCACCAA GGACAAGACC AACCTGATCG TCTACATCAT CTGCGGGGTG
    GTGGCCGTCA TGGTGCTCGT GGGCATCTTC ACCAAGCTGG GGCTGGAGAA AGCGCACCGG
    CCCCAAAGGG AGCACATGTC CAGGGCCCTT GCGGATGTCA TGAGACCACA GGGCCACTGC
    AACACTGATC ACATGGAGAG AGACCTAAAC ATCGTTGTCC ACGTCCAGCA TTATGAGAAC
    ATGGACACGA GAACCCCCAT AAATAATCTT CATGCCACCC AGATGAACAA CGCAGTGCCC
    ACCTCTCCTC TGCTCCAGCA GATGGGCCAT CCACATTCGT ACCCGAACCT GGGCCAGATC
    TCCAACCCCT ATGAACAGCA GCCACCAGGA AAAGAGCTCA ACAAGTACGC CTCCTTAAAG
    GCAGTCGGAA GTTCTGATGG TGACTGGGCA GTATCGACAC TTAAGTCACC AAAAGCTGAC
    AAGGTCAATG ACGACTTCTA CACCAAGCGA CGGCACCTGG CTGAGCTGGC TGCCAAGGGG
    AACTTACCTC TGCACCCCGT AAGAGTGGAG GACGAGCCCC GGGCCTTCAG CCCTGAGCAC
    GGTCCTGCCA AGCAGAATGG ACAGAAGTCC CGCACCAACA AGATGCCCCC ACATCCCCTG
    GCCTACACCT CTACCACCAA CTTTAAGGGC TGGGACCCCA ACGAGCAGTC CCTCCGGCGG
    CAGGCTTACA GCAACAAGGG CAAGCTTGGC ACGGCCGAGA CAGGCTCCAG CGACCCCTTG
    GGAACTCGCC CCCAGCACTA CCCACCCCCA CAGCCATACT TCATCACCAA CAGCAAAACA
    GAAGTGACTG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138676.2
    CDS468..1742
    Translation

    Target ORF information:

    RefSeq Version NM_001145204.3
    Organism Homo sapiens(human)
    Definition Homo sapiens shisa family member 9 (SHISA9), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001145204.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCGCCGCG TCCTTCGGCT GCTCCTCGGT TGCTTCCTCA CCGAGCTGTG CGCCCGCGTG 
    TGCCGGGCGC AGGAGCGAGC GGGACACGGG CAGCTGGCGC AACTGGGCGG CGTGTTGCTG
    CTGGCGGGGG GCAACCGCTC CGGGGCCGCC TCCGGAGAGG CCAGCGAGGG CGCTGAGGCA
    TCGGACGCGC CCCCGACCCG GGCGCCCACG CCGGACTTCT GCCGGGGCTA CTTCGATGTC
    ATGGGCCAGT GGGACCCGCC GTTCAACTGC AGCTCGGGCG ACTTCATCTT CTGCTGCGGG
    ACTTGTGGCT TCCGGTTCTG CTGCACGTTT AAGAAGCGGC GACTGAACCA AAGCACCTGC
    ACCAACTACG ACACGCCGCT CTGGCTCAAC ACCGGCAAGC CCCCCGCCCG CAAGGACGAC
    CCCTTGCACG ACCCCACCAA GGACAAGACC AACCTGATCG TCTACATCAT CTGCGGGGTG
    GTGGCCGTCA TGGTGCTCGT GGGCATCTTC ACCAAGCTGG GGCTGGAGAA AGCGCACCGG
    CCCCAAAGGG AGCACATGTC CAGGGCCCTT GCGGATGTCA TGAGACCACA GGGCCACTGC
    AACACTGATC ACATGGAGAG AGACCTAAAC ATCGTTGTCC ACGTCCAGCA TTATGAGAAC
    ATGGACACGA GAACCCCCAT AAATAATCTT CATGCCACCC AGATGAACAA CGCAGTGCCC
    ACCTCTCCTC TGCTCCAGCA GATGGGCCAT CCACATTCGT ACCCGAACCT GGGCCAGATC
    TCCAACCCCT ATGAACAGCA GCCACCAGGA AAAGAGCTCA ACAAGTACGC CTCCTTAAAG
    GCAGTCGGAA GTTCTGATGG TGACTGGGCA GTATCGACAC TTAAGTCACC AAAAGCTGAC
    AAGGTCAATG ACGACTTCTA CACCAAGCGA CGGCACCTGG CTGAGCTGGC TGCCAAGGGG
    AACTTACCTC TGCACCCCGT AAGAGTGGAG GACGAGCCCC GGGCCTTCAG CCCTGAGCAC
    GGTCCTGCCA AGCAGAATGG ACAGAAGTCC CGCACCAACA AGATGCCCCC ACATCCCCTG
    GCCTACACCT CTACCACCAA CTTTAAGGGC TGGGACCCCA ACGAGCAGTC CCTCCGGCGG
    CAGGCTTACA GCAACAAGGG CAAGCTTGGC ACGGCCGAGA CAGGCTCCAG CGACCCCTTG
    GGAACTCGCC CCCAGCACTA CCCACCCCCA CAGCCATACT TCATCACCAA CAGCAAAACA
    GAAGTGACTG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu15067
    Clone ID Related Accession (Same CDS sequence) NM_001145205.1 , NM_001145205.2
    Accession Version NM_001145205.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 666bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Homo sapiens(human)
    Product protein shisa-9 isoform 2 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK299610.1, AC092324.3, AK309038.1 and CR627300.1. On or before Feb 18, 2009 this sequence version replaced XR_041914.1, XR_041915.1, XR_041913.1, XM_001131993.2, XM_001131913.2, XM_001716437.1. Transcript Variant: This variant (2) uses an alternate terminal exon, compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. This RefSeq contains an in-frame start site 41 codons upstream from the currently annotated site but is not being annotated as a start site since it is in a weak Kozak sequence context. Also, use of the upstream AUG would eliminate the signal peptide. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: CR627300.1, SRR1803617.210872.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCGCCGCG TCCTTCGGCT GCTCCTCGGT TGCTTCCTCA CCGAGCTGTG CGCCCGCGTG 
    TGCCGGGCGC AGGAGCGAGC GGGACACGGG CAGCTGGCGC AACTGGGCGG CGTGTTGCTG
    CTGGCGGGGG GCAACCGCTC CGGGGCCGCC TCCGGAGAGG CCAGCGAGGG CGCTGAGGCA
    TCGGACGCGC CCCCGACCCG GGCGCCCACG CCGGACTTCT GCCGGGGCTA CTTCGATGTC
    ATGGGCCAGT GGGACCCGCC GTTCAACTGC AGCTCGGGCG ACTTCATCTT CTGCTGCGGG
    ACTTGTGGCT TCCGGTTCTG CTGCACGTTT AAGAAGCGGC GACTGAACCA AAGCACCTGC
    ACCAACTACG ACACGCCGCT CTGGCTCAAC ACCGGCAAGC CCCCCGCCCG CAAGGACGAC
    CCCTTGCACG ACCCCACCAA GGACAAGACC AACCTGATCG TCTACATCAT CTGCGGGGTG
    GTGGCCGTCA TGGTGCTCGT GGGCATCTTC ACCAAGCTGG GGCTGGAGAA AGCGCACCGG
    CCCCAAAGGG AGCACATGTC CAGGCTCTAT GACAATCTGC TGTTTATGGA GGCACAGATT
    TCTTTCCAAG AGGACGAACC TGCCCCTGGA GAGTGGAGTG TCGGACTTCA AACCTGGACA
    GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138677.2
    CDS446..1111
    Translation

    Target ORF information:

    RefSeq Version NM_001145205.1
    Organism Homo sapiens(human)
    Definition Homo sapiens shisa family member 9 (SHISA9), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001145205.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCGCCGCG TCCTTCGGCT GCTCCTCGGT TGCTTCCTCA CCGAGCTGTG CGCCCGCGTG 
    TGCCGGGCGC AGGAGCGAGC GGGACACGGG CAGCTGGCGC AACTGGGCGG CGTGTTGCTG
    CTGGCGGGGG GCAACCGCTC CGGGGCCGCC TCCGGAGAGG CCAGCGAGGG CGCTGAGGCA
    TCGGACGCGC CCCCGACCCG GGCGCCCACG CCGGACTTCT GCCGGGGCTA CTTCGATGTC
    ATGGGCCAGT GGGACCCGCC GTTCAACTGC AGCTCGGGCG ACTTCATCTT CTGCTGCGGG
    ACTTGTGGCT TCCGGTTCTG CTGCACGTTT AAGAAGCGGC GACTGAACCA AAGCACCTGC
    ACCAACTACG ACACGCCGCT CTGGCTCAAC ACCGGCAAGC CCCCCGCCCG CAAGGACGAC
    CCCTTGCACG ACCCCACCAA GGACAAGACC AACCTGATCG TCTACATCAT CTGCGGGGTG
    GTGGCCGTCA TGGTGCTCGT GGGCATCTTC ACCAAGCTGG GGCTGGAGAA AGCGCACCGG
    CCCCAAAGGG AGCACATGTC CAGGCTCTAT GACAATCTGC TGTTTATGGA GGCACAGATT
    TCTTTCCAAG AGGACGAACC TGCCCCTGGA GAGTGGAGTG TCGGACTTCA AACCTGGACA
    GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu15067
    Clone ID Related Accession (Same CDS sequence) NM_001145205.1 , NM_001145205.2
    Accession Version NM_001145205.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 666bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product protein shisa-9 isoform 2 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092324.3, AK299610.1, AK309038.1 and CR627300.1. On May 31, 2019 this sequence version replaced NM_001145205.1. Transcript Variant: This variant (2) uses an alternate terminal exon, compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. This RefSeq contains an in-frame start site 41 codons upstream from the currently annotated site but is not being annotated as a start site since it is in a weak Kozak sequence context. Also, use of the upstream AUG would eliminate the signal peptide. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: CR749545.1, CR627300.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCGCCGCG TCCTTCGGCT GCTCCTCGGT TGCTTCCTCA CCGAGCTGTG CGCCCGCGTG 
    TGCCGGGCGC AGGAGCGAGC GGGACACGGG CAGCTGGCGC AACTGGGCGG CGTGTTGCTG
    CTGGCGGGGG GCAACCGCTC CGGGGCCGCC TCCGGAGAGG CCAGCGAGGG CGCTGAGGCA
    TCGGACGCGC CCCCGACCCG GGCGCCCACG CCGGACTTCT GCCGGGGCTA CTTCGATGTC
    ATGGGCCAGT GGGACCCGCC GTTCAACTGC AGCTCGGGCG ACTTCATCTT CTGCTGCGGG
    ACTTGTGGCT TCCGGTTCTG CTGCACGTTT AAGAAGCGGC GACTGAACCA AAGCACCTGC
    ACCAACTACG ACACGCCGCT CTGGCTCAAC ACCGGCAAGC CCCCCGCCCG CAAGGACGAC
    CCCTTGCACG ACCCCACCAA GGACAAGACC AACCTGATCG TCTACATCAT CTGCGGGGTG
    GTGGCCGTCA TGGTGCTCGT GGGCATCTTC ACCAAGCTGG GGCTGGAGAA AGCGCACCGG
    CCCCAAAGGG AGCACATGTC CAGGCTCTAT GACAATCTGC TGTTTATGGA GGCACAGATT
    TCTTTCCAAG AGGACGAACC TGCCCCTGGA GAGTGGAGTG TCGGACTTCA AACCTGGACA
    GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138677.2
    CDS468..1133
    Translation

    Target ORF information:

    RefSeq Version NM_001145205.2
    Organism Homo sapiens(human)
    Definition Homo sapiens shisa family member 9 (SHISA9), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001145205.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCGCCGCG TCCTTCGGCT GCTCCTCGGT TGCTTCCTCA CCGAGCTGTG CGCCCGCGTG 
    TGCCGGGCGC AGGAGCGAGC GGGACACGGG CAGCTGGCGC AACTGGGCGG CGTGTTGCTG
    CTGGCGGGGG GCAACCGCTC CGGGGCCGCC TCCGGAGAGG CCAGCGAGGG CGCTGAGGCA
    TCGGACGCGC CCCCGACCCG GGCGCCCACG CCGGACTTCT GCCGGGGCTA CTTCGATGTC
    ATGGGCCAGT GGGACCCGCC GTTCAACTGC AGCTCGGGCG ACTTCATCTT CTGCTGCGGG
    ACTTGTGGCT TCCGGTTCTG CTGCACGTTT AAGAAGCGGC GACTGAACCA AAGCACCTGC
    ACCAACTACG ACACGCCGCT CTGGCTCAAC ACCGGCAAGC CCCCCGCCCG CAAGGACGAC
    CCCTTGCACG ACCCCACCAA GGACAAGACC AACCTGATCG TCTACATCAT CTGCGGGGTG
    GTGGCCGTCA TGGTGCTCGT GGGCATCTTC ACCAAGCTGG GGCTGGAGAA AGCGCACCGG
    CCCCAAAGGG AGCACATGTC CAGGCTCTAT GACAATCTGC TGTTTATGGA GGCACAGATT
    TCTTTCCAAG AGGACGAACC TGCCCCTGGA GAGTGGAGTG TCGGACTTCA AACCTGGACA
    GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu80904
    Clone ID Related Accession (Same CDS sequence) XM_005255539.3
    Accession Version XM_005255539.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1350bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein shisa-9 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005255539.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTGCGGCC CGCGGCGGCT CGCAGCTCCC GGCAGCAGCC TCGGCAGCTT CGGCCGCGCC 
    TCGAGAGGCG GCCGCAGAGG CTCCAGCGGC GGCCGAGCGG CCGAGCCCGG GCTGGGAGAC
    ACCATGCGCC GCGTCCTTCG GCTGCTCCTC GGTTGCTTCC TCACCGAGCT GTGCGCCCGC
    GTGTGCCGGG CGCAGGAGCG AGCGGGACAC GGGCAGCTGG CGCAACTGGG CGGCGTGTTG
    CTGCTGGCGG GGGGCAACCG CTCCGGGGCC GCCTCCGGAG AGGCCAGCGA GGGCGCTGAG
    GCATCGGACG CGCCCCCGAC CCGGGCGCCC ACGCCGGACT TCTGCCGGGG CTACTTCGAT
    GTCATGGGCC AGTGGGACCC GCCGTTCAAC TGCAGCTCGG GCGACTTCAT CTTCTGCTGC
    GGGACTTGTG GCTTCCGGTT CTGCTGCACG TTTAAGAAGC GGCGACTGAA CCAAAGCACC
    TGCACCAACT ACGACACGCC GCTCTGGCTC AACACCGGCA AGCCCCCCGC CCGCAAGGAC
    GACCCCTTGC ACGACCCCAC CAAGGACAAG ACCAACCTGA TCGTCTACAT CATCTGCGGG
    GTGGTGGCCG TCATGGTGCT CGTGGGCATC TTCACCAAGC TGGGGCTGGA GAAAGCGCAC
    CGGCCCCAAA GGGAGCACAT GTCCAGGGCC CTTGCGGATG TCATGAGACC ACAGGGCCAC
    TGCAACACTG ATCACATGGA GAGAGACCTA AACATCGTTG TCCACGTCCA GCATTATGAG
    AACATGGACA CGAGAACCCC CATAAATAAT CTTCATGCCA CCCAGATGAA CAACGCAGTG
    CCCACCTCTC CTCTGCTCCA GCAGATGGGC CATCCACATT CGTACCCGAA CCTGGGCCAG
    ATCTCCAACC CCTATGAACA GCAGCCACCA GGAAAAGAGC TCAACAAGTA CGCCTCCTTA
    AAGGCAGTCG CTGACAAGGT CAATGACGAC TTCTACACCA AGCGACGGCA CCTGGCTGAG
    CTGGCTGCCA AGGGGAACTT ACCTCTGCAC CCCGTAAGAG TGGAGGACGA GCCCCGGGCC
    TTCAGCCCTG AGCACGGTCC TGCCAAGCAG AATGGACAGA AGTCCCGCAC CAACAAGATG
    CCCCCACATC CCCTGGCCTA CACCTCTACC ACCAACTTTA AGGGCTGGGA CCCCAACGAG
    CAGTCCCTCC GGCGGCAGGC TTACAGCAAC AAGGGCAAGC TTGGCACGGC CGAGACAGGC
    TCCAGCGACC CCTTGGGAAC TCGCCCCCAG CACTACCCAC CCCCACAGCC ATACTTCATC
    ACCAACAGCA AAACAGAAGT GACTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005255596.2
    CDS324..1673
    Translation

    Target ORF information:

    RefSeq Version XM_005255539.3
    Organism Homo sapiens(human)
    Definition Homo sapiens shisa family member 9 (SHISA9), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005255539.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTGCGGCC CGCGGCGGCT CGCAGCTCCC GGCAGCAGCC TCGGCAGCTT CGGCCGCGCC 
    TCGAGAGGCG GCCGCAGAGG CTCCAGCGGC GGCCGAGCGG CCGAGCCCGG GCTGGGAGAC
    ACCATGCGCC GCGTCCTTCG GCTGCTCCTC GGTTGCTTCC TCACCGAGCT GTGCGCCCGC
    GTGTGCCGGG CGCAGGAGCG AGCGGGACAC GGGCAGCTGG CGCAACTGGG CGGCGTGTTG
    CTGCTGGCGG GGGGCAACCG CTCCGGGGCC GCCTCCGGAG AGGCCAGCGA GGGCGCTGAG
    GCATCGGACG CGCCCCCGAC CCGGGCGCCC ACGCCGGACT TCTGCCGGGG CTACTTCGAT
    GTCATGGGCC AGTGGGACCC GCCGTTCAAC TGCAGCTCGG GCGACTTCAT CTTCTGCTGC
    GGGACTTGTG GCTTCCGGTT CTGCTGCACG TTTAAGAAGC GGCGACTGAA CCAAAGCACC
    TGCACCAACT ACGACACGCC GCTCTGGCTC AACACCGGCA AGCCCCCCGC CCGCAAGGAC
    GACCCCTTGC ACGACCCCAC CAAGGACAAG ACCAACCTGA TCGTCTACAT CATCTGCGGG
    GTGGTGGCCG TCATGGTGCT CGTGGGCATC TTCACCAAGC TGGGGCTGGA GAAAGCGCAC
    CGGCCCCAAA GGGAGCACAT GTCCAGGGCC CTTGCGGATG TCATGAGACC ACAGGGCCAC
    TGCAACACTG ATCACATGGA GAGAGACCTA AACATCGTTG TCCACGTCCA GCATTATGAG
    AACATGGACA CGAGAACCCC CATAAATAAT CTTCATGCCA CCCAGATGAA CAACGCAGTG
    CCCACCTCTC CTCTGCTCCA GCAGATGGGC CATCCACATT CGTACCCGAA CCTGGGCCAG
    ATCTCCAACC CCTATGAACA GCAGCCACCA GGAAAAGAGC TCAACAAGTA CGCCTCCTTA
    AAGGCAGTCG CTGACAAGGT CAATGACGAC TTCTACACCA AGCGACGGCA CCTGGCTGAG
    CTGGCTGCCA AGGGGAACTT ACCTCTGCAC CCCGTAAGAG TGGAGGACGA GCCCCGGGCC
    TTCAGCCCTG AGCACGGTCC TGCCAAGCAG AATGGACAGA AGTCCCGCAC CAACAAGATG
    CCCCCACATC CCCTGGCCTA CACCTCTACC ACCAACTTTA AGGGCTGGGA CCCCAACGAG
    CAGTCCCTCC GGCGGCAGGC TTACAGCAAC AAGGGCAAGC TTGGCACGGC CGAGACAGGC
    TCCAGCGACC CCTTGGGAAC TCGCCCCCAG CACTACCCAC CCCCACAGCC ATACTTCATC
    ACCAACAGCA AAACAGAAGT GACTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu80905
    Clone ID Related Accession (Same CDS sequence) XM_011522642.2
    Accession Version XM_011522642.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein shisa-9 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522642.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGTGCGGCC CGCGGCGGCT CGCAGCTCCC GGCAGCAGCC TCGGCAGCTT CGGCCGCGCC 
    TCGAGAGGCG GCCGCAGAGG CTCCAGCGGC GGCCGAGCGG CCGAGCCCGG GCTGGGAGAC
    ACCATGCGCC GCGTCCTTCG GCTGCTCCTC GGTTGCTTCC TCACCGAGCT GTGCGCCCGC
    GTGTGCCGGG CGCAGGAGCG AGCGGGACAC GGGCAGCTGG CGCAACTGGG CGGCGTGTTG
    CTGCTGGCGG GGGGCAACCG CTCCGGGGCC GCCTCCGGAG AGGCCAGCGA GGGCGCTGAG
    GCATCGGACG CGCCCCCGAC CCGGGCGCCC ACGCCGGACT TCTGCCGGGG CTACTTCGAT
    GTCATGGGCC AGTGGGACCC GCCGTTCAAC TGCAGCTCGG GCGACTTCAT CTTCTGCTGC
    GGGACTTGTG GCTTCCGGTT CTGCTGCACG TTTAAGAAGC GGCGACTGAA CCAAAGCACC
    TGCACCAACT ACGACACGCC GCTCTGGCTC AACACCGGCA AGCCCCCCGC CCGCAAGGAC
    GACCCCTTGC ACGACCCCAC CAAGGACAAG ACCAACCTGA TCGTCTACAT CATCTGCGGG
    GTGGTGGCCG TCATGGTGCT CGTGGGCATC TTCACCAAGC TGGGGCTGGA GAAAGCGCAC
    CGGCCCCAAA GGGAGCACAT GTCCAGGGCC CTTGCGGATG TCATGAGACC ACAGGGCCAC
    TGCAACACTG ATCACATGGA GAGAGACCTA AACATCGTTG TCCACGTCCA GCATTATGAG
    AACATGGACA CGAGAACCCC CATAAATAAT CTTCATGCCA CCCAGATGAA CAACGCAGTG
    CCCACCTCTC CTCTGCTCCA GCAGATGGGC CATCCACATT CGTACCCGAA CCTGGGCCAG
    ATCTCCAACC CCTATGAACA GCAGCCACCA GGAAAAGAGC TCAACAAGTA CGCCTCCTTA
    AAGGCAGTCG AGCTGGAACA CCCTTCTTCT CCTGCCTTTG GACATCAGAA CTTCAGATTC
    TCTGGCCTTC AGACTTCAAG ACTTGCACTA GTGGCCCCCT GGGTTCTCAA GGTTTTGGCC
    GCCTCGGTTG AGAGTTACAC CATCGGCTTC TTTGGTTCTG AGGCCGTTGG AGTTGGACTG
    AGCCATGCTA CCAGCTTCCC TGGGTCTCCA GCCTGCAGAT TGCCTACTGT GAGATTTAGC
    CTCCATAATC ACGTAACAGA ATATTGGCAT GTAGCACTCC TCAAAACATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011520944.1
    CDS324..1574
    Translation

    Target ORF information:

    RefSeq Version XM_011522642.2
    Organism Homo sapiens(human)
    Definition Homo sapiens shisa family member 9 (SHISA9), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011522642.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGTGCGGCC CGCGGCGGCT CGCAGCTCCC GGCAGCAGCC TCGGCAGCTT CGGCCGCGCC 
    TCGAGAGGCG GCCGCAGAGG CTCCAGCGGC GGCCGAGCGG CCGAGCCCGG GCTGGGAGAC
    ACCATGCGCC GCGTCCTTCG GCTGCTCCTC GGTTGCTTCC TCACCGAGCT GTGCGCCCGC
    GTGTGCCGGG CGCAGGAGCG AGCGGGACAC GGGCAGCTGG CGCAACTGGG CGGCGTGTTG
    CTGCTGGCGG GGGGCAACCG CTCCGGGGCC GCCTCCGGAG AGGCCAGCGA GGGCGCTGAG
    GCATCGGACG CGCCCCCGAC CCGGGCGCCC ACGCCGGACT TCTGCCGGGG CTACTTCGAT
    GTCATGGGCC AGTGGGACCC GCCGTTCAAC TGCAGCTCGG GCGACTTCAT CTTCTGCTGC
    GGGACTTGTG GCTTCCGGTT CTGCTGCACG TTTAAGAAGC GGCGACTGAA CCAAAGCACC
    TGCACCAACT ACGACACGCC GCTCTGGCTC AACACCGGCA AGCCCCCCGC CCGCAAGGAC
    GACCCCTTGC ACGACCCCAC CAAGGACAAG ACCAACCTGA TCGTCTACAT CATCTGCGGG
    GTGGTGGCCG TCATGGTGCT CGTGGGCATC TTCACCAAGC TGGGGCTGGA GAAAGCGCAC
    CGGCCCCAAA GGGAGCACAT GTCCAGGGCC CTTGCGGATG TCATGAGACC ACAGGGCCAC
    TGCAACACTG ATCACATGGA GAGAGACCTA AACATCGTTG TCCACGTCCA GCATTATGAG
    AACATGGACA CGAGAACCCC CATAAATAAT CTTCATGCCA CCCAGATGAA CAACGCAGTG
    CCCACCTCTC CTCTGCTCCA GCAGATGGGC CATCCACATT CGTACCCGAA CCTGGGCCAG
    ATCTCCAACC CCTATGAACA GCAGCCACCA GGAAAAGAGC TCAACAAGTA CGCCTCCTTA
    AAGGCAGTCG AGCTGGAACA CCCTTCTTCT CCTGCCTTTG GACATCAGAA CTTCAGATTC
    TCTGGCCTTC AGACTTCAAG ACTTGCACTA GTGGCCCCCT GGGTTCTCAA GGTTTTGGCC
    GCCTCGGTTG AGAGTTACAC CATCGGCTTC TTTGGTTCTG AGGCCGTTGG AGTTGGACTG
    AGCCATGCTA CCAGCTTCCC TGGGTCTCCA GCCTGCAGAT TGCCTACTGT GAGATTTAGC
    CTCCATAATC ACGTAACAGA ATATTGGCAT GTAGCACTCC TCAAAACATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.