RPL22 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPL22
Entrez Gene ID	6146
Full Name	ribosomal protein L22
Synonyms	EAP,HBP15,HBP15/L22,L22
General protein information	Preferred Names ribosomal protein L22 Names 60S ribosomal protein L22 EBER-associated protein Epstein-Barr virus small RNA-associated protein Epstein-Barr-encoded RNA-associated protein heparin-binding protein 15 heparin-binding protein HBp15 large ribosomal subunit protein eL22
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.31
Summary	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22E family of ribosomal proteins. Its initiating methionine residue is post-translationally removed. The protein can bind specifically to Epstein-Barr virus-encoded RNAs (EBERs) 1 and 2. The mouse protein has been shown to be capable of binding to heparin. Transcript variants utilizing alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. It was previously thought that this gene mapped to 3q26 and that it was fused to the acute myeloid leukemia 1 (AML1) gene located at 21q22 in some therapy-related myelodysplastic syndrome patients with 3;21 translocations; however, these fusions actually involve a ribosomal protein L22 pseudogene located at 3q26, and this gene actually maps to 1p36.3-p36.2. [provided by RefSeq, Jul 2008].
Disorder MIM:	180474

mRNA and Protein(s)

mRNA	Protein	Name
NM_000983.4	NP_000974.1	60S ribosomal protein L22
NM_000983.3	NP_000974.1	60S ribosomal protein L22

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	RpL22	NP_477134.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005046	XP_313917.3
Magnaporthe oryzae (rice blast fungus)	MGG_04104	XP_003719728.1
Arabidopsis thaliana (thale cress)	AT3G05560	NP_187207.1
Schizosaccharomyces pombe (fission yeast)	rpl22	NP_594940.1
Rattus norvegicus (Norway rat)	Rpl22	NP_112366.1
Caenorhabditis elegans (roundworm)	rpl-22	NP_494932.1
Neurospora crassa	NCU06661	XP_960908.1
Oryza sativa (rice)	Os03g0343500	NP_001050078.1
Bos taurus (cattle)	RPL22	XP_002694159.1
Gallus gallus (chicken)	RPL22	NP_989472.1
Kluyveromyces lactis	KLLA0D05181g	XP_453291.1
Arabidopsis thaliana (thale cress)	AT5G27770	NP_198129.1
Oryza sativa (rice)	Os07g0674200	NP_001060613.1
Homo sapiens (human)	RPL22	NP_000974.1
Pan troglodytes (chimpanzee)	RPL22	XP_003949328.1
	RPL22	XP_001091945.1
Canis lupus familiaris (dog)	RPL22	XP_849312.1
Mus musculus (house mouse)	Rpl22	NP_033105.1
Saccharomyces cerevisiae (baker's yeast)	RPL22A	NP_013162.1
Eremothecium gossypii	AGOS_ACR221W	NP_983623.1

Gene Ontology
Bibliography

Related articles in PubMed

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

High frequency of RPL22 mutations in microsatellite-unstable colorectal and endometrial tumors.
Ferreira AM, Tuominen I, van Dijk-Bos K, Sanjabi B, van der Sluis T, van der Zee AG, Hollema H, Zazula M, Sijmons RH, Aaltonen LA, Westers H, Hofstra RM
Human mutation35(12)1442-5(2014 Dec)

Interaction of ribosomal protein L22 with casein kinase 2?: a novel mechanism for understanding the biology of non-small cell lung cancer.
Yang M, Sun H, He J, Wang H, Yu X, Ma L, Zhu C
Oncology reports32(1)139-44(2014 Jul)

SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor angiogenic markers.
Zanivan S, Maione F, Hein MY, Hern?ndez-Fernaud JR, Ostasiewicz P, Giraudo E, Mann M
Molecular & cellular proteomics : MCP12(12)3599-611(2013 Dec)

Down-regulation of ribosomal protein L22 in non-small cell lung cancer.
Yang M, Sun H, Wang H, Zhang S, Yu X, Zhang L
Medical oncology (Northwood, London, England)30(3)646(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

considering the high mutation frequency found, our data point toward an important role for RPL22 in microsatellite instability carcinogenesis
Title: High frequency of RPL22 mutations in microsatellite-unstable colorectal and endometrial tumors.

Study revealved that RPL22 binds CK2alpha in lung cancer cells.
Title: Interaction of ribosomal protein L22 with casein kinase 2?: a novel mechanism for understanding the biology of non-small cell lung cancer.

RPL22 is frequently mutated in MSI-high endometrioid endometrial cancers. The A8 mutation identified was not reported in the whole exome sequences analyzed by the TCGA.
Title: Frequent mutations in the RPL22 gene and its clinical and functional implications.

Down-regulation of RPL22 might be involved in the carcinogenesis of NSCLC.
Title: Down-regulation of ribosomal protein L22 in non-small cell lung cancer.

Rpl22 inactivation promotes neoplastic transformation by inducing expression of Lin28B
Title: Inactivation of ribosomal protein L22 promotes transformation by induction of the stemness factor, Lin28B.

The following RPL22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPL22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16614	NM_000983.4 Latest version!	Homo sapiens ribosomal protein L22 (RPL22), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu16614	NM_000983.3	Homo sapiens ribosomal protein L22 (RPL22), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPL22 ( NM_000983.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000974.1 Homo sapiens ribosomal protein L22 (RPL22), mRNA.

Price & Availability↑

CloneID	OHu16614
Clone ID Related Accession (Same CDS sequence)	NM_000983.4 , NM_000983.3
Accession Version	NM_000983.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	60S ribosomal protein L22
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from CA336134.1, AK097075.1 and AF113701.1. On Nov 23, 2018 this sequence version replaced NM_000983.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.436838.1, SRR3476690.1039763.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000234875.9/ ENSP00000346088.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTCCTG TGAAAAAGCT TGTGGTGAAG GGGGGCAAAA AAAAGAAGCA AGTTCTGAAG 
TTCACTCTTG ATTGCACCCA CCCTGTAGAA GATGGAATCA TGGATGCTGC CAATTTTGAG 
CAGTTTTTGC AAGAAAGGAT CAAAGTGAAC GGAAAAGCTG GGAACCTTGG TGGAGGGGTG 
GTGACCATCG AAAGGAGCAA GAGCAAGATC ACCGTGACAT CCGAGGTGCC TTTCTCCAAA 
AGGTATTTGA AATATCTCAC CAAAAAATAT TTGAAGAAGA ATAATCTACG TGACTGGTTG 
CGCGTAGTTG CTAACAGCAA AGAGAGTTAC GAATTACGTT ACTTCCAGAT TAACCAGGAC 
GAAGAAGAGG AGGAAGACGA GGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000974.1
CDS	23..409
Translation	MAPVKKLVVKGGKKKKQVLKFTLDCTHPVEDGIMDAANFEQFLQERIKVNGKAGNLGGGVVTIERSKSKITVTSEVPFSKRYLKYLTKKYLKKNNLRDWLRVVANSKESYELRYFQINQDEEEEEDED

Target ORF information:

RefSeq Version	NM_000983.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L22 (RPL22), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000983.4

ORF Insert Sequence:

ATGGCTCCTG TGAAAAAGCT TGTGGTGAAG GGGGGCAAAA AAAAGAAGCA AGTTCTGAAG 
TTCACTCTTG ATTGCACCCA CCCTGTAGAA GATGGAATCA TGGATGCTGC CAATTTTGAG 
CAGTTTTTGC AAGAAAGGAT CAAAGTGAAC GGAAAAGCTG GGAACCTTGG TGGAGGGGTG 
GTGACCATCG AAAGGAGCAA GAGCAAGATC ACCGTGACAT CCGAGGTGCC TTTCTCCAAA 
AGGTATTTGA AATATCTCAC CAAAAAATAT TTGAAGAAGA ATAATCTACG TGACTGGTTG 
CGCGTAGTTG CTAACAGCAA AGAGAGTTAC GAATTACGTT ACTTCCAGAT TAACCAGGAC 
GAAGAAGAGG AGGAAGACGA GGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL22 ( NM_000983.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000974.1 Homo sapiens ribosomal protein L22 (RPL22), mRNA.

Price & Availability↑

CloneID	OHu16614
Clone ID Related Accession (Same CDS sequence)	NM_000983.4 , NM_000983.3
Accession Version	NM_000983.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	60S ribosomal protein L22
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from CA336134.1, AK097075.1 and AF113701.1. On Jun 4, 2004 this sequence version replaced NM_000983.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.22016.1, SRR1803613.176654.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCTCCTG TGAAAAAGCT TGTGGTGAAG GGGGGCAAAA AAAAGAAGCA AGTTCTGAAG 
TTCACTCTTG ATTGCACCCA CCCTGTAGAA GATGGAATCA TGGATGCTGC CAATTTTGAG 
CAGTTTTTGC AAGAAAGGAT CAAAGTGAAC GGAAAAGCTG GGAACCTTGG TGGAGGGGTG 
GTGACCATCG AAAGGAGCAA GAGCAAGATC ACCGTGACAT CCGAGGTGCC TTTCTCCAAA 
AGGTATTTGA AATATCTCAC CAAAAAATAT TTGAAGAAGA ATAATCTACG TGACTGGTTG 
CGCGTAGTTG CTAACAGCAA AGAGAGTTAC GAATTACGTT ACTTCCAGAT TAACCAGGAC 
GAAGAAGAGG AGGAAGACGA GGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000974.1
CDS	47..433
Translation	MAPVKKLVVKGGKKKKQVLKFTLDCTHPVEDGIMDAANFEQFLQERIKVNGKAGNLGGGVVTIERSKSKITVTSEVPFSKRYLKYLTKKYLKKNNLRDWLRVVANSKESYELRYFQINQDEEEEEDED

Target ORF information:

RefSeq Version	NM_000983.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L22 (RPL22), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000983.3

ORF Insert Sequence:

ATGGCTCCTG TGAAAAAGCT TGTGGTGAAG GGGGGCAAAA AAAAGAAGCA AGTTCTGAAG 
TTCACTCTTG ATTGCACCCA CCCTGTAGAA GATGGAATCA TGGATGCTGC CAATTTTGAG 
CAGTTTTTGC AAGAAAGGAT CAAAGTGAAC GGAAAAGCTG GGAACCTTGG TGGAGGGGTG 
GTGACCATCG AAAGGAGCAA GAGCAAGATC ACCGTGACAT CCGAGGTGCC TTTCTCCAAA 
AGGTATTTGA AATATCTCAC CAAAAAATAT TTGAAGAAGA ATAATCTACG TGACTGGTTG 
CGCGTAGTTG CTAACAGCAA AGAGAGTTAC GAATTACGTT ACTTCCAGAT TAACCAGGAC 
GAAGAAGAGG AGGAAGACGA GGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP

High frequency of RPL22 mutations in microsatellite-unstable colorectal and endometrial tumors.
Human mutation35(12)1442-5(2014 Dec)
Ferreira AM,Tuominen I,van Dijk-Bos K,Sanjabi B,van der Sluis T,van der Zee AG,Hollema H,Zazula M,Sijmons RH,Aaltonen LA,Westers H,Hofstra RM

Interaction of ribosomal protein L22 with casein kinase 2?: a novel mechanism for understanding the biology of non-small cell lung cancer.
Oncology reports32(1)139-44(2014 Jul)
Yang M,Sun H,He J,Wang H,Yu X,Ma L,Zhu C

SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor angiogenic markers.
Molecular & cellular proteomics : MCP12(12)3599-611(2013 Dec)
Zanivan S,Maione F,Hein MY,Hern?ndez-Fernaud JR,Ostasiewicz P,Giraudo E,Mann M

Down-regulation of ribosomal protein L22 in non-small cell lung cancer.
Medical oncology (Northwood, London, England)30(3)646(2013)
Yang M,Sun H,Wang H,Zhang S,Yu X,Zhang L

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RPL22 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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