PGAP6 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PGAP6
Entrez Gene ID	58986
Full Name	transmembrane protein 8A
Synonyms	GPI-PLA2,M83,PGAP6,TMEM6,TMEM8
General protein information	Preferred Names transmembrane protein 8A Names post-GPI attachment to proteins factor 6 GPI processing phospholipase A2 five-span transmembrane protein M83 post-glycosylphosphatidylinositol attachment to proteins 6 transmembrane protein 6 transmembrane protein 8 (five membrane-spanning domains)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p13.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_024450366.1	XP_024306134.1	post-GPI attachment to proteins factor 6 isoform X1
NM_021259.2	NP_067082.2	post-GPI attachment to proteins factor 6 precursor
NM_021259.3	NP_067082.2	post-GPI attachment to proteins factor 6 precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TMEM8A	NP_001092377.1
Mus musculus (house mouse)	Tmem8	NP_068565.1
	TMEM8A	XP_001118461.1
Canis lupus familiaris (dog)	TMEM8A	XP_003639163.1
Homo sapiens (human)	TMEM8A	NP_067082.2
Pan troglodytes (chimpanzee)	TMEM8A	XP_510709.3
Rattus norvegicus (Norway rat)	Tmem8a	NP_001100461.1

Gene Ontology
Bibliography

Related articles in PubMed

A GPI processing phospholipase A2, PGAP6, modulates Nodal signaling in embryos by shedding CRIPTO.
Lee GH, Fujita M, Takaoka K, Murakami Y, Fujihara Y, Kanzawa N, Murakami KI, Kajikawa E, Takada Y, Saito K, Ikawa M, Hamada H, Maeda Y, Kinoshita T
The Journal of cell biology215(5)705-718(2016 Dec)

Characterization of the CLEAR network reveals an integrated control of cellular clearance pathways.
Palmieri M, Impey S, Kang H, di Ronza A, Pelz C, Sardiello M, Ballabio A
Human molecular genetics20(19)3852-66(2011 Oct)

Integral and associated lysosomal membrane proteins.
Schr?der B, Wrocklage C, Pan C, J?ger R, K?sters B, Sch?fer H, Els?sser HP, Mann M, Hasilik A
Traffic (Copenhagen, Denmark)8(12)1676-86(2007 Dec)

The sequence and analysis of duplication-rich human chromosome 16.
Martin J, Han C, Gordon LA, Terry A, Prabhakar S, She X, Xie G, Hellsten U, Chan YM, Altherr M, Couronne O, Aerts A, Bajorek E, Black S, Blumer H, Branscomb E, Brown NC, Bruno WJ, Buckingham JM, Callen DF, Campbell CS, Campbell ML, Campbell EW, Caoile C, Challacombe JF, Chasteen LA, Chertkov O, Chi HC, Christensen M, Clark LM, Cohn JD, Denys M, Detter JC, Dickson M, Dimitrijevic-Bussod M, Escobar J, Fawcett JJ, Flowers D, Fotopulos D, Glavina T, Gomez M, Gonzales E, Goodstein D, Goodwin LA, Grady DL, Grigoriev I, Groza M, Hammon N, Hawkins T, Haydu L, Hildebrand CE, Huang W, Israni S, Jett J, Jewett PB, Kadner K, Kimball H, Kobayashi A, Krawczyk MC, Leyba T, Longmire JL, Lopez F, Lou Y, Lowry S, Ludeman T, Manohar CF, Mark GA, McMurray KL, Meincke LJ, Morgan J, Moyzis RK, Mundt MO, Munk AC, Nandkeshwar RD, Pitluck S, Pollard M, Predki P, Parson-Quintana B, Ramirez L, Rash S, Retterer J, Ricke DO, Robinson DL, Rodriguez A, Salamov A, Saunders EH, Scott D, Shough T, Stallings RL, Stalvey M, Sutherland RD, Tapia R, Tesmer JG, Thayer N, Thompson LS, Tice H, Torney DC, Tran-Gyamfi M, Tsai M, Ulanovsky LE, Ustaszewska A, Vo N, White PS, Williams AL, Wills PL, Wu JR, Wu K, Yang J, Dejong P, Bruce D, Doggett NA, Deaven L, Schmutz J, Grimwood J, Richardson P, Rokhsar DS, Eichler EE, Gilna P, Lucas SM, Myers RM, Rubin EM, Pennacchio LA
Nature432(7020)988-94(2004 Dec)

Sequence, structure and pathology of the fully annotated terminal 2 Mb of the short arm of human chromosome 16.
Daniels RJ, Peden JF, Lloyd C, Horsley SW, Clark K, Tufarelli C, Kearney L, Buckle VJ, Doggett NA, Flint J, Higgs DR
Human molecular genetics10(4)339-52(2001 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PGAP6 plays a critical role in Nodal signaling modulation through CRIPTO shedding.
Title: A GPI processing phospholipase A2, PGAP6, modulates Nodal signaling in embryos by shedding CRIPTO.

TMEM8A protein localizes in lysosomes in HeLa cells
Title: Characterization of the CLEAR network reveals an integrated control of cellular clearance pathways.

The following PGAP6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PGAP6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03712	NM_021259.3 Latest version!	Homo sapiens post-glycosylphosphatidylinositol attachment to proteins 6 (PGAP6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu104181	XM_024450366.1 Latest version!	Homo sapiens post-glycosylphosphatidylinositol attachment to proteins 6 (PGAP6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu03712	NM_021259.2	Homo sapiens transmembrane protein 8A (TMEM8A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PGAP6 ( NM_021259.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_067082.2 Homo sapiens post-glycosylphosphatidylinositol attachment to proteins 6 (PGAP6), mRNA.

Price & Availability↑

CloneID	OHu03712
Clone ID Related Accession (Same CDS sequence)	NM_021259.2 , NM_021259.3
Accession Version	NM_021259.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-05
Organism	Homo sapiens(human)
Product	post-GPI attachment to proteins factor 6 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from Z97634.26, AL537021.3, AB045292.1, BC021557.1, DB224383.1, AL546643.3, BC004276.1 and AI678371.1. On Nov 23, 2018 this sequence version replaced NM_021259.2. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.225897.1, AB045292.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000431232.7/ ENSP00000401338.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCCGGG CTGGCACCGG GACCGGGGGC GAGGCGGTGG CCGCGGTGGT GGCGGGGCCG 
CTGCTGCTGC TGCTGCTTGC CCGGCCCCCG CCTGCCTCCG CCGGCTACAG CGGGAAGAGC 
GAGGTGGGGC TGGTGTCCGA GCACTTCTCG CAGGCCCCGC AGAGGCTGTC CTTCTACAGC 
TGGTACGGCA GTGCCAGGCT CTTCCGCTTC CGCGTGCCCC CAGATGCTGT GCTTCTACGC 
TGGCTCCTGC AGGTCTCCCG GGAGAGCGGC GCTGCCTGCA CCGACGCGGA GATCACCGTG 
CACTTCCGTT CCGGCGCCCC TCCGGTCATC AACCCGCTGG GCACCAGCTT CCCGGACGAC 
ACCGCGGTAC AGCCCTCCTT CCAGGTCGGG GTGCCGCTGA GCACCACACC GAGAAGCAAT 
GCCTCCGTCA ACGTTTCCCA CCCGGCCCCC GGGGACTGGT TCGTGGCCGC CCACCTGCCC 
CCCTCATCCC AGAAGATCGA GTTGAAGGGC TTGGCTCCCA CCTGTGCCTA CGTCTTCCAG 
CCTGAACTGC TGGTCACGCG GGTGGTCGAG ATTTCCATCA TGGAGCCGGA CGTGCCCCTT 
CCTCAGACCC TCCTCTCCCA TCCCAGCTAC CTCAAGGTCT TTGTCCCCGA TTACACGCGG 
GAGCTTCTGC TGGAGCTGCG GGACTGCGTG TCCAATGGGA GCCTGGGCTG CCCCGTGCGT 
CTCACCGTGG GCCCGGTCAC CCTGCCTAGC AACTTCCAGA AGGTGCTCAC CTGCACCGGT 
GCCCCCTGGC CCTGCCGCCT GCTGCTGCCC TCACCGCCCT GGGACCGGTG GCTGCAAGTG 
ACAGCTGAGA GCCTGGTGGG GCCCCTCGGG ACAGTGGCTT TCAGTGCTGT AGCTGCCCTC 
ACAGCTTGCA GGCCACGGAG CGTGACCATC CAGCCCCTTC TGCAGAGCAG CCAAAACCAG 
AGCTTCAATG CCTCCTCTGG TCTGCTGTCC CCGAGCCCCG ACCACCAGGA CCTGGGCAGG 
AGTGGCAGGG TGGACCGCAG CCCCTTCTGC CTCACAAACT ACCCAGTCAC GCGGGAGGAC 
ATGGACGTGG TGTCGGTGCA CTTCCAGCCC CTGGACAGGG TCTCGGTGAG GGTGTGTTCG 
GACACGCCCT CCGTGATGCG GCTGCGCCTG AACACCGGCA TGGACAGCGG GGGTTCCCTC 
ACCATCTCCC TGCGGGCCAA CAAGACAGAG ATGCGGAACG AGACCGTCGT AGTGGCCTGC 
GTGAATGCTG CCTCGCCCTT CCTTGGCTTC AATACTTCGC TCAACTGCAC CACAGCCTTC 
TTCCAGGGCT ACCCTTTGTC TCTGAGCGCC TGGTCTCGCA GGGCCAACCT CATCATCCCC 
TACCCAGAGA CAGACAACTG GTACCTCTCC CTGCAGCTCA TGTGCCCTGA GAATGCTGAG 
GACTGTGAGC AGGCTGTGGT CCACGTGGAG ACCACCTTGT ACCTGGTGCC CTGTTTGAAC 
GATTGTGGAC CCTATGGCCA GTGCCTCCTG CTCCGCAGAC ACAGCTACCT GTATGCCAGC 
TGCAGCTGCA AGGCAGGCTG GCGTGGGTGG AGCTGCACGG ACAACAGCAC AGCCCAGACG 
GTGGCCCAGC AGAGGGCGGC CACACTGCTG CTCACGCTCA GCAACCTCAT GTTCCTGGCC 
CCCATCGCCG TCTCAGTGCG GCGATTCTTC CTGGTGGAGG CCTCCGTCTA CGCCTACACC 
ATGTTCTTCT CCACGTTCTA CCACGCCTGC GACCAGCCCG GGGAGGCGGT GCTGTGCATC 
CTCAGCTACG ACACGCTGCA GTACTGCGAC TTCTTGGGCT CCGGGGCGGC CATCTGGGTC 
ACCATCCTGT GCATGGCACG GCTCAAGACA GTCCTGAAAT ACGTGCTGTT TCTTCTGGGT 
ACACTGGTCA TCGCCATGTC CTTGCAGCTG GACCGCAGGG GCATGTGGAA CATGCTGGGG 
CCCTGCCTCT TTGCCTTCGT GATCATGGCC TCCATGTGGG CTTACCGCTG CGGGCACCGG 
CGCCAGTGCT ACCCCACCTC GTGGCAGCGC TGGGCCTTCT ACCTCCTGCC CGGCGTCTCT 
ATGGCCTCTG TGGGCATCGC CATCTACACC TCCATGATGA CTAGCGACAA CTACTACTAC 
ACCCACAGCA TCTGGCACAT CCTGCTGGCC GGGAGCGCAG CCTTGCTGCT GCCGCCACCT 
GACCAGCCCG CCGAGCCCTG GGCCTGCTCG CAGAAATTCC CCTGCCACTA TCAGATCTGC 
AAGAACGATC GGGAGGAACT GTACGCAGTG ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067082.2
CDS	158..2473
Translation	MGRAGTGTGGEAVAAVVAGPLLLLLLARPPPASAGYSGKSEVGLVSEHFSQAPQRLSFYSWYGSARLFRFRVPPDAVLLRWLLQVSRESGAACTDAEITVHFRSGAPPVINPLGTSFPDDTAVQPSFQVGVPLSTTPRSNASVNVSHPAPGDWFVAAHLPPSSQKIELKGLAPTCAYVFQPELLVTRVVEISIMEPDVPLPQTLLSHPSYLKVFVPDYTRELLLELRDCVSNGSLGCPVRLTVGPVTLPSNFQKVLTCTGAPWPCRLLLPSPPWDRWLQVTAESLVGPLGTVAFSAVAALTACRPRSVTIQPLLQSSQNQSFNASSGLLSPSPDHQDLGRSGRVDRSPFCLTNYPVTREDMDVVSVHFQPLDRVSVRVCSDTPSVMRLRLNTGMDSGGSLTISLRANKTEMRNETVVVACVNAASPFLGFNTSLNCTTAFFQGYPLSLSAWSRRANLIIPYPETDNWYLSLQLMCPENAEDCEQAVVHVETTLYLVPCLNDCGPYGQCLLLRRHSYLYASCSCKAGWRGWSCTDNSTAQTVAQQRAATLLLTLSNLMFLAPIAVSVRRFFLVEASVYAYTMFFSTFYHACDQPGEAVLCILSYDTLQYCDFLGSGAAIWVTILCMARLKTVLKYVLFLLGTLVIAMSLQLDRRGMWNMLGPCLFAFVIMASMWAYRCGHRRQCYPTSWQRWAFYLLPGVSMASVGIAIYTSMMTSDNYYYTHSIWHILLAGSAALLLPPPDQPAEPWACSQKFPCHYQICKNDREELYAVT

Target ORF information:

RefSeq Version	NM_021259.3
Organism	Homo sapiens(human)
Definition	Homo sapiens post-glycosylphosphatidylinositol attachment to proteins 6 (PGAP6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021259.3

ORF Insert Sequence:

ATGGGCCGGG CTGGCACCGG GACCGGGGGC GAGGCGGTGG CCGCGGTGGT GGCGGGGCCG 
CTGCTGCTGC TGCTGCTTGC CCGGCCCCCG CCTGCCTCCG CCGGCTACAG CGGGAAGAGC 
GAGGTGGGGC TGGTGTCCGA GCACTTCTCG CAGGCCCCGC AGAGGCTGTC CTTCTACAGC 
TGGTACGGCA GTGCCAGGCT CTTCCGCTTC CGCGTGCCCC CAGATGCTGT GCTTCTACGC 
TGGCTCCTGC AGGTCTCCCG GGAGAGCGGC GCTGCCTGCA CCGACGCGGA GATCACCGTG 
CACTTCCGTT CCGGCGCCCC TCCGGTCATC AACCCGCTGG GCACCAGCTT CCCGGACGAC 
ACCGCGGTAC AGCCCTCCTT CCAGGTCGGG GTGCCGCTGA GCACCACACC GAGAAGCAAT 
GCCTCCGTCA ACGTTTCCCA CCCGGCCCCC GGGGACTGGT TCGTGGCCGC CCACCTGCCC 
CCCTCATCCC AGAAGATCGA GTTGAAGGGC TTGGCTCCCA CCTGTGCCTA CGTCTTCCAG 
CCTGAACTGC TGGTCACGCG GGTGGTCGAG ATTTCCATCA TGGAGCCGGA CGTGCCCCTT 
CCTCAGACCC TCCTCTCCCA TCCCAGCTAC CTCAAGGTCT TTGTCCCCGA TTACACGCGG 
GAGCTTCTGC TGGAGCTGCG GGACTGCGTG TCCAATGGGA GCCTGGGCTG CCCCGTGCGT 
CTCACCGTGG GCCCGGTCAC CCTGCCTAGC AACTTCCAGA AGGTGCTCAC CTGCACCGGT 
GCCCCCTGGC CCTGCCGCCT GCTGCTGCCC TCACCGCCCT GGGACCGGTG GCTGCAAGTG 
ACAGCTGAGA GCCTGGTGGG GCCCCTCGGG ACAGTGGCTT TCAGTGCTGT AGCTGCCCTC 
ACAGCTTGCA GGCCACGGAG CGTGACCATC CAGCCCCTTC TGCAGAGCAG CCAAAACCAG 
AGCTTCAATG CCTCCTCTGG TCTGCTGTCC CCGAGCCCCG ACCACCAGGA CCTGGGCAGG 
AGTGGCAGGG TGGACCGCAG CCCCTTCTGC CTCACAAACT ACCCAGTCAC GCGGGAGGAC 
ATGGACGTGG TGTCGGTGCA CTTCCAGCCC CTGGACAGGG TCTCGGTGAG GGTGTGTTCG 
GACACGCCCT CCGTGATGCG GCTGCGCCTG AACACCGGCA TGGACAGCGG GGGTTCCCTC 
ACCATCTCCC TGCGGGCCAA CAAGACAGAG ATGCGGAACG AGACCGTCGT AGTGGCCTGC 
GTGAATGCTG CCTCGCCCTT CCTTGGCTTC AATACTTCGC TCAACTGCAC CACAGCCTTC 
TTCCAGGGCT ACCCTTTGTC TCTGAGCGCC TGGTCTCGCA GGGCCAACCT CATCATCCCC 
TACCCAGAGA CAGACAACTG GTACCTCTCC CTGCAGCTCA TGTGCCCTGA GAATGCTGAG 
GACTGTGAGC AGGCTGTGGT CCACGTGGAG ACCACCTTGT ACCTGGTGCC CTGTTTGAAC 
GATTGTGGAC CCTATGGCCA GTGCCTCCTG CTCCGCAGAC ACAGCTACCT GTATGCCAGC 
TGCAGCTGCA AGGCAGGCTG GCGTGGGTGG AGCTGCACGG ACAACAGCAC AGCCCAGACG 
GTGGCCCAGC AGAGGGCGGC CACACTGCTG CTCACGCTCA GCAACCTCAT GTTCCTGGCC 
CCCATCGCCG TCTCAGTGCG GCGATTCTTC CTGGTGGAGG CCTCCGTCTA CGCCTACACC 
ATGTTCTTCT CCACGTTCTA CCACGCCTGC GACCAGCCCG GGGAGGCGGT GCTGTGCATC 
CTCAGCTACG ACACGCTGCA GTACTGCGAC TTCTTGGGCT CCGGGGCGGC CATCTGGGTC 
ACCATCCTGT GCATGGCACG GCTCAAGACA GTCCTGAAAT ACGTGCTGTT TCTTCTGGGT 
ACACTGGTCA TCGCCATGTC CTTGCAGCTG GACCGCAGGG GCATGTGGAA CATGCTGGGG 
CCCTGCCTCT TTGCCTTCGT GATCATGGCC TCCATGTGGG CTTACCGCTG CGGGCACCGG 
CGCCAGTGCT ACCCCACCTC GTGGCAGCGC TGGGCCTTCT ACCTCCTGCC CGGCGTCTCT 
ATGGCCTCTG TGGGCATCGC CATCTACACC TCCATGATGA CTAGCGACAA CTACTACTAC 
ACCCACAGCA TCTGGCACAT CCTGCTGGCC GGGAGCGCAG CCTTGCTGCT GCCGCCACCT 
GACCAGCCCG CCGAGCCCTG GGCCTGCTCG CAGAAATTCC CCTGCCACTA TCAGATCTGC 
AAGAACGATC GGGAGGAACT GTACGCAGTG ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PGAP6 ( XM_024450366.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306134.1 Homo sapiens post-glycosylphosphatidylinositol attachment to proteins 6 (PGAP6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu104181
Clone ID Related Accession (Same CDS sequence)	XM_024450366.1
Accession Version	XM_024450366.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	post-GPI attachment to proteins factor 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCGGG CTGGCACCGG GACCGGGGGC GAGGCGGTGG CCGCGGTGGT GGCGGGGCCG 
CTGCTGCTGC TGCTGCTTGC CCGGCCCCCG CCTGCCTCCG CCGGCTACAG CGGGAAGAGC 
GAGGTGGGGC TGGTGTCCGA GCACTTCTCG CAGGCCCCGC AGAGGCTGTC CTTCTACAGC 
TGGTACGGCA GTGCCAGGCT CTTCCGCTTC CGCGTGCCCC CAGATGCTGT GCTTCTACGC 
TGGCTCCTGC AGGTCTCCCG GGAGAGCGGC GCTGCCTGCA CCGACGCGGA GATCACCGTG 
CACTTCCGTT CCGGCGCCCC TCCGGTCATC AACCCGCTGG GCACCAGCTT CCCGGACGAC 
ACCGCGGTAC AGCCCTCCTT CCAGGTCGGG GTGCCGCTGA GCACCACACC GAGAAGCAAT 
GCCTCCGTCA ACGTTTCCCA CCCGGCCCCC GGGGACTGGT TCGTGGCCGC CCACCTGCCC 
CCCTCATCCC AGAAGATCGA GTTGAAGGGC TTGGCTCCCA CCTGTGCCTA CGTCTTCCAG 
CCTGAACTGC TGGTCACGCG GGTGGTCGAG ATTTCCATCA TGGAGCCGGA CGTGCCCCTT 
CCTCAGACCC TCCTCTCCCA TCCCAGCTAC CTCAAGGTCT TTGTCCCCGA TTACACGCGG 
GAGCTTCTGC TGGAGCTGCG GGACTGCGTG TCCAATGGGA GCCTGGGCTG CCCCGTGCGT 
CTCACCGTGG GCCCGGTCAC CCTGCCTAGC AACTTCCAGA AGGTGCTCAC CTGCACCGGT 
GCCCCCTGGC CCTGCCGCCT GCTGCTGCCC TCACCGCCCT GGGACCGGTG GCTGCAAGTG 
ACAGCTGAGA GCCTGGTGGG GCCCCTCGGG ACAGTGGCTT TCAGTGCTGT AGCTGCCCTC 
ACAGCTTGCA GGCCACGGAG CGTGACCATC CAGCCCCTTC TGCAGAGCAG CCAAAACCAG 
AGCTTCAATG CCTCCTCTGG TCTGCTGTCC CCGAGCCCCG ACCACCAGGA CCTGGGCAGG 
AGTGGCAGGG TGGACCGCAG CCCCTTCTGC CTCACAAACT ACCCAGTCAC GCGGGAGGAC 
ATGGACGTGG TGTCGGTGCA CTTCCAGCCC CTGGACAGGG TCTCGGTGAG GGTGTGTTCG 
GACACGCCCT CCGTGATGCG GCTGCGCCTG AACACCGGCA TGGACAGCGG GGGTTCCCTC 
ACCATCTCCC TGCGGGCCAA CAAGACAGAG ATGCGGAACG AGACCGTCGT AGTGGCCTGC 
GTGAATGCTG CCTCGCCCTT CCTTGGCTTC AATACTTCGC TCAACTGCAC CACAGCCTTC 
TTCCAGGGCT ACCCTTTGTC TCTGAGCGCC TGGTCTCGCA GGGCCAACCT CATCATCCCC 
TACCCAGAGA CAGACAACTG GTACCTCTCC CTGCAGCTCA TGTGCCCTGA GAATGCTGAG 
GACTGTGAGC AGGCTGTGGT CCACGTGGAG ACCACCTTGT ACCTGGTGCC CTGTTTGAAC 
GATTGTGGAC CCTATGGCCA GTGCCTCCTG CTCCGCAGAC ACAGCTACCT GTATGCCAGC 
TGCAGCTGCA AGGCAGGCTG GCGTGGGTGG AGCTGCACGG ACAACAGCAC AGCCCAGACG 
GTGGCCCAGC AGAGGGCGGC CACACTGCTG CTCACGCTCA GCAACCTCAT GTTCCTGGCC 
CCCATCGCCG TCTCAGTGCG GCGATTCTTC CTGGTGGAGG CCTCCGTCTA CGCCTACACC 
ATGTTCTTCT CCACGGTAGG CTGGCCCCTC CTGCAGGGAT GTGGGGTGGG AGGGCAGGAC 
CGCGCTCATG GAGCCTCCCC ACAGTTCTAC CACGCCTGCG ACCAGCCCGG GGAGGCGGTG 
CTGTGCATCC TCAGCTACGA CACGCTGCAG TACTGCGACT TCTTGGGCTC CGGGGCGGCC 
ATCTGGGTCA CCATCCTGTG CATGGCACGG CTCAAGACAG TCCTGAAATA CGTGCTGTTT 
CTTCTGGGTA CACTGGTCAT CGCCATGTCC TTGCAGCTGG ACCGCAGGGG CATGTGGAAC 
ATGCTGGGGC CCTGCCTCTT TGCCTTCGTG ATCATGGCCT CCATGTGGGC TTACCGCTGC 
GGGCACCGGC GCCAGTGCTA CCCCACCTCG TGGCAGCGCT GGGCCTTCTA CCTCCTGCCC 
GGCGTCTCTA TGGCCTCTGT GGGCATCGCC ATCTACACCT CCATGATGAC TAGCGACAAC 
TACTACTACA CCCACAGCAT CTGGCACATC CTGCTGGCCG GGAGCGCAGC CTTGCTGCTG 
CCGCCACCTG ACCAGCCCGC CGAGCCCTGG GCCTGCTCGC AGAAATTCCC CTGCCACTAT 
CAGATCTGCA AGAACGATCG GGAGGAACTG TACGCAGTGA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306134.1
CDS	130..2514
Translation	MGRAGTGTGGEAVAAVVAGPLLLLLLARPPPASAGYSGKSEVGLVSEHFSQAPQRLSFYSWYGSARLFRFRVPPDAVLLRWLLQVSRESGAACTDAEITVHFRSGAPPVINPLGTSFPDDTAVQPSFQVGVPLSTTPRSNASVNVSHPAPGDWFVAAHLPPSSQKIELKGLAPTCAYVFQPELLVTRVVEISIMEPDVPLPQTLLSHPSYLKVFVPDYTRELLLELRDCVSNGSLGCPVRLTVGPVTLPSNFQKVLTCTGAPWPCRLLLPSPPWDRWLQVTAESLVGPLGTVAFSAVAALTACRPRSVTIQPLLQSSQNQSFNASSGLLSPSPDHQDLGRSGRVDRSPFCLTNYPVTREDMDVVSVHFQPLDRVSVRVCSDTPSVMRLRLNTGMDSGGSLTISLRANKTEMRNETVVVACVNAASPFLGFNTSLNCTTAFFQGYPLSLSAWSRRANLIIPYPETDNWYLSLQLMCPENAEDCEQAVVHVETTLYLVPCLNDCGPYGQCLLLRRHSYLYASCSCKAGWRGWSCTDNSTAQTVAQQRAATLLLTLSNLMFLAPIAVSVRRFFLVEASVYAYTMFFSTVGWPLLQGCGVGGQDRAHGASPQFYHACDQPGEAVLCILSYDTLQYCDFLGSGAAIWVTILCMARLKTVLKYVLFLLGTLVIAMSLQLDRRGMWNMLGPCLFAFVIMASMWAYRCGHRRQCYPTSWQRWAFYLLPGVSMASVGIAIYTSMMTSDNYYYTHSIWHILLAGSAALLLPPPDQPAEPWACSQKFPCHYQICKNDREELYAVT

Target ORF information:

RefSeq Version	XM_024450366.1
Organism	Homo sapiens(human)
Definition	Homo sapiens post-glycosylphosphatidylinositol attachment to proteins 6 (PGAP6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450366.1

ORF Insert Sequence:

ATGGGCCGGG CTGGCACCGG GACCGGGGGC GAGGCGGTGG CCGCGGTGGT GGCGGGGCCG 
CTGCTGCTGC TGCTGCTTGC CCGGCCCCCG CCTGCCTCCG CCGGCTACAG CGGGAAGAGC 
GAGGTGGGGC TGGTGTCCGA GCACTTCTCG CAGGCCCCGC AGAGGCTGTC CTTCTACAGC 
TGGTACGGCA GTGCCAGGCT CTTCCGCTTC CGCGTGCCCC CAGATGCTGT GCTTCTACGC 
TGGCTCCTGC AGGTCTCCCG GGAGAGCGGC GCTGCCTGCA CCGACGCGGA GATCACCGTG 
CACTTCCGTT CCGGCGCCCC TCCGGTCATC AACCCGCTGG GCACCAGCTT CCCGGACGAC 
ACCGCGGTAC AGCCCTCCTT CCAGGTCGGG GTGCCGCTGA GCACCACACC GAGAAGCAAT 
GCCTCCGTCA ACGTTTCCCA CCCGGCCCCC GGGGACTGGT TCGTGGCCGC CCACCTGCCC 
CCCTCATCCC AGAAGATCGA GTTGAAGGGC TTGGCTCCCA CCTGTGCCTA CGTCTTCCAG 
CCTGAACTGC TGGTCACGCG GGTGGTCGAG ATTTCCATCA TGGAGCCGGA CGTGCCCCTT 
CCTCAGACCC TCCTCTCCCA TCCCAGCTAC CTCAAGGTCT TTGTCCCCGA TTACACGCGG 
GAGCTTCTGC TGGAGCTGCG GGACTGCGTG TCCAATGGGA GCCTGGGCTG CCCCGTGCGT 
CTCACCGTGG GCCCGGTCAC CCTGCCTAGC AACTTCCAGA AGGTGCTCAC CTGCACCGGT 
GCCCCCTGGC CCTGCCGCCT GCTGCTGCCC TCACCGCCCT GGGACCGGTG GCTGCAAGTG 
ACAGCTGAGA GCCTGGTGGG GCCCCTCGGG ACAGTGGCTT TCAGTGCTGT AGCTGCCCTC 
ACAGCTTGCA GGCCACGGAG CGTGACCATC CAGCCCCTTC TGCAGAGCAG CCAAAACCAG 
AGCTTCAATG CCTCCTCTGG TCTGCTGTCC CCGAGCCCCG ACCACCAGGA CCTGGGCAGG 
AGTGGCAGGG TGGACCGCAG CCCCTTCTGC CTCACAAACT ACCCAGTCAC GCGGGAGGAC 
ATGGACGTGG TGTCGGTGCA CTTCCAGCCC CTGGACAGGG TCTCGGTGAG GGTGTGTTCG 
GACACGCCCT CCGTGATGCG GCTGCGCCTG AACACCGGCA TGGACAGCGG GGGTTCCCTC 
ACCATCTCCC TGCGGGCCAA CAAGACAGAG ATGCGGAACG AGACCGTCGT AGTGGCCTGC 
GTGAATGCTG CCTCGCCCTT CCTTGGCTTC AATACTTCGC TCAACTGCAC CACAGCCTTC 
TTCCAGGGCT ACCCTTTGTC TCTGAGCGCC TGGTCTCGCA GGGCCAACCT CATCATCCCC 
TACCCAGAGA CAGACAACTG GTACCTCTCC CTGCAGCTCA TGTGCCCTGA GAATGCTGAG 
GACTGTGAGC AGGCTGTGGT CCACGTGGAG ACCACCTTGT ACCTGGTGCC CTGTTTGAAC 
GATTGTGGAC CCTATGGCCA GTGCCTCCTG CTCCGCAGAC ACAGCTACCT GTATGCCAGC 
TGCAGCTGCA AGGCAGGCTG GCGTGGGTGG AGCTGCACGG ACAACAGCAC AGCCCAGACG 
GTGGCCCAGC AGAGGGCGGC CACACTGCTG CTCACGCTCA GCAACCTCAT GTTCCTGGCC 
CCCATCGCCG TCTCAGTGCG GCGATTCTTC CTGGTGGAGG CCTCCGTCTA CGCCTACACC 
ATGTTCTTCT CCACGGTAGG CTGGCCCCTC CTGCAGGGAT GTGGGGTGGG AGGGCAGGAC 
CGCGCTCATG GAGCCTCCCC ACAGTTCTAC CACGCCTGCG ACCAGCCCGG GGAGGCGGTG 
CTGTGCATCC TCAGCTACGA CACGCTGCAG TACTGCGACT TCTTGGGCTC CGGGGCGGCC 
ATCTGGGTCA CCATCCTGTG CATGGCACGG CTCAAGACAG TCCTGAAATA CGTGCTGTTT 
CTTCTGGGTA CACTGGTCAT CGCCATGTCC TTGCAGCTGG ACCGCAGGGG CATGTGGAAC 
ATGCTGGGGC CCTGCCTCTT TGCCTTCGTG ATCATGGCCT CCATGTGGGC TTACCGCTGC 
GGGCACCGGC GCCAGTGCTA CCCCACCTCG TGGCAGCGCT GGGCCTTCTA CCTCCTGCCC 
GGCGTCTCTA TGGCCTCTGT GGGCATCGCC ATCTACACCT CCATGATGAC TAGCGACAAC 
TACTACTACA CCCACAGCAT CTGGCACATC CTGCTGGCCG GGAGCGCAGC CTTGCTGCTG 
CCGCCACCTG ACCAGCCCGC CGAGCCCTGG GCCTGCTCGC AGAAATTCCC CTGCCACTAT 
CAGATCTGCA AGAACGATCG GGAGGAACTG TACGCAGTGA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PGAP6 ( NM_021259.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_067082.2 Homo sapiens transmembrane protein 8A (TMEM8A), mRNA.

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CloneID	OHu03712
Clone ID Related Accession (Same CDS sequence)	NM_021259.2 , NM_021259.3
Accession Version	NM_021259.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-09
Organism	Homo sapiens(human)
Product	post-GPI attachment to proteins factor 6 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL537021.3, AB045292.1, BC021557.1, DB224383.1, AL546643.3, BC004276.1 and AI678371.1. On Sep 26, 2007 this sequence version replaced NM_021259.1. ##Evidence-Data-START## Transcript exon combination :: AB045292.1, SRR1163655.225897.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGGCCGGG CTGGCACCGG GACCGGGGGC GAGGCGGTGG CCGCGGTGGT GGCGGGGCCG 
CTGCTGCTGC TGCTGCTTGC CCGGCCCCCG CCTGCCTCCG CCGGCTACAG CGGGAAGAGC 
GAGGTGGGGC TGGTGTCCGA GCACTTCTCG CAGGCCCCGC AGAGGCTGTC CTTCTACAGC 
TGGTACGGCA GTGCCAGGCT CTTCCGCTTC CGCGTGCCCC CAGATGCTGT GCTTCTACGC 
TGGCTCCTGC AGGTCTCCCG GGAGAGCGGC GCTGCCTGCA CCGACGCGGA GATCACCGTG 
CACTTCCGTT CCGGCGCCCC TCCGGTCATC AACCCGCTGG GCACCAGCTT CCCGGACGAC 
ACCGCGGTAC AGCCCTCCTT CCAGGTCGGG GTGCCGCTGA GCACCACACC GAGAAGCAAT 
GCCTCCGTCA ACGTTTCCCA CCCGGCCCCC GGGGACTGGT TCGTGGCCGC CCACCTGCCC 
CCCTCATCCC AGAAGATCGA GTTGAAGGGC TTGGCTCCCA CCTGTGCCTA CGTCTTCCAG 
CCTGAACTGC TGGTCACGCG GGTGGTCGAG ATTTCCATCA TGGAGCCGGA CGTGCCCCTT 
CCTCAGACCC TCCTCTCCCA TCCCAGCTAC CTCAAGGTCT TTGTCCCCGA TTACACGCGG 
GAGCTTCTGC TGGAGCTGCG GGACTGCGTG TCCAATGGGA GCCTGGGCTG CCCCGTGCGT 
CTCACCGTGG GCCCGGTCAC CCTGCCTAGC AACTTCCAGA AGGTGCTCAC CTGCACCGGT 
GCCCCCTGGC CCTGCCGCCT GCTGCTGCCC TCACCGCCCT GGGACCGGTG GCTGCAAGTG 
ACAGCTGAGA GCCTGGTGGG GCCCCTCGGG ACAGTGGCTT TCAGTGCTGT AGCTGCCCTC 
ACAGCTTGCA GGCCACGGAG CGTGACCATC CAGCCCCTTC TGCAGAGCAG CCAAAACCAG 
AGCTTCAATG CCTCCTCTGG TCTGCTGTCC CCGAGCCCCG ACCACCAGGA CCTGGGCAGG 
AGTGGCAGGG TGGACCGCAG CCCCTTCTGC CTCACAAACT ACCCAGTCAC GCGGGAGGAC 
ATGGACGTGG TGTCGGTGCA CTTCCAGCCC CTGGACAGGG TCTCGGTGAG GGTGTGTTCG 
GACACGCCCT CCGTGATGCG GCTGCGCCTG AACACCGGCA TGGACAGCGG GGGTTCCCTC 
ACCATCTCCC TGCGGGCCAA CAAGACAGAG ATGCGGAACG AGACCGTCGT AGTGGCCTGC 
GTGAATGCTG CCTCGCCCTT CCTTGGCTTC AATACTTCGC TCAACTGCAC CACAGCCTTC 
TTCCAGGGCT ACCCTTTGTC TCTGAGCGCC TGGTCTCGCA GGGCCAACCT CATCATCCCC 
TACCCAGAGA CAGACAACTG GTACCTCTCC CTGCAGCTCA TGTGCCCTGA GAATGCTGAG 
GACTGTGAGC AGGCTGTGGT CCACGTGGAG ACCACCTTGT ACCTGGTGCC CTGTTTGAAC 
GATTGTGGAC CCTATGGCCA GTGCCTCCTG CTCCGCAGAC ACAGCTACCT GTATGCCAGC 
TGCAGCTGCA AGGCAGGCTG GCGTGGGTGG AGCTGCACGG ACAACAGCAC AGCCCAGACG 
GTGGCCCAGC AGAGGGCGGC CACACTGCTG CTCACGCTCA GCAACCTCAT GTTCCTGGCC 
CCCATCGCCG TCTCAGTGCG GCGATTCTTC CTGGTGGAGG CCTCCGTCTA CGCCTACACC 
ATGTTCTTCT CCACGTTCTA CCACGCCTGC GACCAGCCCG GGGAGGCGGT GCTGTGCATC 
CTCAGCTACG ACACGCTGCA GTACTGCGAC TTCTTGGGCT CCGGGGCGGC CATCTGGGTC 
ACCATCCTGT GCATGGCACG GCTCAAGACA GTCCTGAAAT ACGTGCTGTT TCTTCTGGGT 
ACACTGGTCA TCGCCATGTC CTTGCAGCTG GACCGCAGGG GCATGTGGAA CATGCTGGGG 
CCCTGCCTCT TTGCCTTCGT GATCATGGCC TCCATGTGGG CTTACCGCTG CGGGCACCGG 
CGCCAGTGCT ACCCCACCTC GTGGCAGCGC TGGGCCTTCT ACCTCCTGCC CGGCGTCTCT 
ATGGCCTCTG TGGGCATCGC CATCTACACC TCCATGATGA CTAGCGACAA CTACTACTAC 
ACCCACAGCA TCTGGCACAT CCTGCTGGCC GGGAGCGCAG CCTTGCTGCT GCCGCCACCT 
GACCAGCCCG CCGAGCCCTG GGCCTGCTCG CAGAAATTCC CCTGCCACTA TCAGATCTGC 
AAGAACGATC GGGAGGAACT GTACGCAGTG ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067082.2
CDS	130..2445
Translation	MGRAGTGTGGEAVAAVVAGPLLLLLLARPPPASAGYSGKSEVGLVSEHFSQAPQRLSFYSWYGSARLFRFRVPPDAVLLRWLLQVSRESGAACTDAEITVHFRSGAPPVINPLGTSFPDDTAVQPSFQVGVPLSTTPRSNASVNVSHPAPGDWFVAAHLPPSSQKIELKGLAPTCAYVFQPELLVTRVVEISIMEPDVPLPQTLLSHPSYLKVFVPDYTRELLLELRDCVSNGSLGCPVRLTVGPVTLPSNFQKVLTCTGAPWPCRLLLPSPPWDRWLQVTAESLVGPLGTVAFSAVAALTACRPRSVTIQPLLQSSQNQSFNASSGLLSPSPDHQDLGRSGRVDRSPFCLTNYPVTREDMDVVSVHFQPLDRVSVRVCSDTPSVMRLRLNTGMDSGGSLTISLRANKTEMRNETVVVACVNAASPFLGFNTSLNCTTAFFQGYPLSLSAWSRRANLIIPYPETDNWYLSLQLMCPENAEDCEQAVVHVETTLYLVPCLNDCGPYGQCLLLRRHSYLYASCSCKAGWRGWSCTDNSTAQTVAQQRAATLLLTLSNLMFLAPIAVSVRRFFLVEASVYAYTMFFSTFYHACDQPGEAVLCILSYDTLQYCDFLGSGAAIWVTILCMARLKTVLKYVLFLLGTLVIAMSLQLDRRGMWNMLGPCLFAFVIMASMWAYRCGHRRQCYPTSWQRWAFYLLPGVSMASVGIAIYTSMMTSDNYYYTHSIWHILLAGSAALLLPPPDQPAEPWACSQKFPCHYQICKNDREELYAVT

Target ORF information:

RefSeq Version	NM_021259.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 8A (TMEM8A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021259.2

ORF Insert Sequence:

ATGGGCCGGG CTGGCACCGG GACCGGGGGC GAGGCGGTGG CCGCGGTGGT GGCGGGGCCG 
CTGCTGCTGC TGCTGCTTGC CCGGCCCCCG CCTGCCTCCG CCGGCTACAG CGGGAAGAGC 
GAGGTGGGGC TGGTGTCCGA GCACTTCTCG CAGGCCCCGC AGAGGCTGTC CTTCTACAGC 
TGGTACGGCA GTGCCAGGCT CTTCCGCTTC CGCGTGCCCC CAGATGCTGT GCTTCTACGC 
TGGCTCCTGC AGGTCTCCCG GGAGAGCGGC GCTGCCTGCA CCGACGCGGA GATCACCGTG 
CACTTCCGTT CCGGCGCCCC TCCGGTCATC AACCCGCTGG GCACCAGCTT CCCGGACGAC 
ACCGCGGTAC AGCCCTCCTT CCAGGTCGGG GTGCCGCTGA GCACCACACC GAGAAGCAAT 
GCCTCCGTCA ACGTTTCCCA CCCGGCCCCC GGGGACTGGT TCGTGGCCGC CCACCTGCCC 
CCCTCATCCC AGAAGATCGA GTTGAAGGGC TTGGCTCCCA CCTGTGCCTA CGTCTTCCAG 
CCTGAACTGC TGGTCACGCG GGTGGTCGAG ATTTCCATCA TGGAGCCGGA CGTGCCCCTT 
CCTCAGACCC TCCTCTCCCA TCCCAGCTAC CTCAAGGTCT TTGTCCCCGA TTACACGCGG 
GAGCTTCTGC TGGAGCTGCG GGACTGCGTG TCCAATGGGA GCCTGGGCTG CCCCGTGCGT 
CTCACCGTGG GCCCGGTCAC CCTGCCTAGC AACTTCCAGA AGGTGCTCAC CTGCACCGGT 
GCCCCCTGGC CCTGCCGCCT GCTGCTGCCC TCACCGCCCT GGGACCGGTG GCTGCAAGTG 
ACAGCTGAGA GCCTGGTGGG GCCCCTCGGG ACAGTGGCTT TCAGTGCTGT AGCTGCCCTC 
ACAGCTTGCA GGCCACGGAG CGTGACCATC CAGCCCCTTC TGCAGAGCAG CCAAAACCAG 
AGCTTCAATG CCTCCTCTGG TCTGCTGTCC CCGAGCCCCG ACCACCAGGA CCTGGGCAGG 
AGTGGCAGGG TGGACCGCAG CCCCTTCTGC CTCACAAACT ACCCAGTCAC GCGGGAGGAC 
ATGGACGTGG TGTCGGTGCA CTTCCAGCCC CTGGACAGGG TCTCGGTGAG GGTGTGTTCG 
GACACGCCCT CCGTGATGCG GCTGCGCCTG AACACCGGCA TGGACAGCGG GGGTTCCCTC 
ACCATCTCCC TGCGGGCCAA CAAGACAGAG ATGCGGAACG AGACCGTCGT AGTGGCCTGC 
GTGAATGCTG CCTCGCCCTT CCTTGGCTTC AATACTTCGC TCAACTGCAC CACAGCCTTC 
TTCCAGGGCT ACCCTTTGTC TCTGAGCGCC TGGTCTCGCA GGGCCAACCT CATCATCCCC 
TACCCAGAGA CAGACAACTG GTACCTCTCC CTGCAGCTCA TGTGCCCTGA GAATGCTGAG 
GACTGTGAGC AGGCTGTGGT CCACGTGGAG ACCACCTTGT ACCTGGTGCC CTGTTTGAAC 
GATTGTGGAC CCTATGGCCA GTGCCTCCTG CTCCGCAGAC ACAGCTACCT GTATGCCAGC 
TGCAGCTGCA AGGCAGGCTG GCGTGGGTGG AGCTGCACGG ACAACAGCAC AGCCCAGACG 
GTGGCCCAGC AGAGGGCGGC CACACTGCTG CTCACGCTCA GCAACCTCAT GTTCCTGGCC 
CCCATCGCCG TCTCAGTGCG GCGATTCTTC CTGGTGGAGG CCTCCGTCTA CGCCTACACC 
ATGTTCTTCT CCACGTTCTA CCACGCCTGC GACCAGCCCG GGGAGGCGGT GCTGTGCATC 
CTCAGCTACG ACACGCTGCA GTACTGCGAC TTCTTGGGCT CCGGGGCGGC CATCTGGGTC 
ACCATCCTGT GCATGGCACG GCTCAAGACA GTCCTGAAAT ACGTGCTGTT TCTTCTGGGT 
ACACTGGTCA TCGCCATGTC CTTGCAGCTG GACCGCAGGG GCATGTGGAA CATGCTGGGG 
CCCTGCCTCT TTGCCTTCGT GATCATGGCC TCCATGTGGG CTTACCGCTG CGGGCACCGG 
CGCCAGTGCT ACCCCACCTC GTGGCAGCGC TGGGCCTTCT ACCTCCTGCC CGGCGTCTCT 
ATGGCCTCTG TGGGCATCGC CATCTACACC TCCATGATGA CTAGCGACAA CTACTACTAC 
ACCCACAGCA TCTGGCACAT CCTGCTGGCC GGGAGCGCAG CCTTGCTGCT GCCGCCACCT 
GACCAGCCCG CCGAGCCCTG GGCCTGCTCG CAGAAATTCC CCTGCCACTA TCAGATCTGC 
AAGAACGATC GGGAGGAACT GTACGCAGTG ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A GPI processing phospholipase A2, PGAP6, modulates Nodal signaling in embryos by shedding CRIPTO.
The Journal of cell biology215(5)705-718(2016 Dec)
Lee GH,Fujita M,Takaoka K,Murakami Y,Fujihara Y,Kanzawa N,Murakami KI,Kajikawa E,Takada Y,Saito K,Ikawa M,Hamada H,Maeda Y,Kinoshita T

Characterization of the CLEAR network reveals an integrated control of cellular clearance pathways.
Human molecular genetics20(19)3852-66(2011 Oct)
Palmieri M,Impey S,Kang H,di Ronza A,Pelz C,Sardiello M,Ballabio A

Integral and associated lysosomal membrane proteins.
Traffic (Copenhagen, Denmark)8(12)1676-86(2007 Dec)
Schr?der B,Wrocklage C,Pan C,J?ger R,K?sters B,Sch?fer H,Els?sser HP,Mann M,Hasilik A

The sequence and analysis of duplication-rich human chromosome 16.
Nature432(7020)988-94(2004 Dec)
Martin J,Han C,Gordon LA,Terry A,Prabhakar S,She X,Xie G,Hellsten U,Chan YM,Altherr M,Couronne O,Aerts A,Bajorek E,Black S,Blumer H,Branscomb E,Brown NC,Bruno WJ,Buckingham JM,Callen DF,Campbell CS,Campbell ML,Campbell EW,Caoile C,Challacombe JF,Chasteen LA,Chertkov O,Chi HC,Christensen M,Clark LM,Cohn JD,Denys M,Detter JC,Dickson M,Dimitrijevic-Bussod M,Escobar J,Fawcett JJ,Flowers D,Fotopulos D,Glavina T,Gomez M,Gonzales E,Goodstein D,Goodwin LA,Grady DL,Grigoriev I,Groza M,Hammon N,Hawkins T,Haydu L,Hildebrand CE,Huang W,Israni S,Jett J,Jewett PB,Kadner K,Kimball H,Kobayashi A,Krawczyk MC,Leyba T,Longmire JL,Lopez F,Lou Y,Lowry S,Ludeman T,Manohar CF,Mark GA,McMurray KL,Meincke LJ,Morgan J,Moyzis RK,Mundt MO,Munk AC,Nandkeshwar RD,Pitluck S,Pollard M,Predki P,Parson-Quintana B,Ramirez L,Rash S,Retterer J,Ricke DO,Robinson DL,Rodriguez A,Salamov A,Saunders EH,Scott D,Shough T,Stallings RL,Stalvey M,Sutherland RD,Tapia R,Tesmer JG,Thayer N,Thompson LS,Tice H,Torney DC,Tran-Gyamfi M,Tsai M,Ulanovsky LE,Ustaszewska A,Vo N,White PS,Williams AL,Wills PL,Wu JR,Wu K,Yang J,Dejong P,Bruce D,Doggett NA,Deaven L,Schmutz J,Grimwood J,Richardson P,Rokhsar DS,Eichler EE,Gilna P,Lucas SM,Myers RM,Rubin EM,Pennacchio LA

Sequence, structure and pathology of the fully annotated terminal 2 Mb of the short arm of human chromosome 16.
Human molecular genetics10(4)339-52(2001 Feb)
Daniels RJ,Peden JF,Lloyd C,Horsley SW,Clark K,Tufarelli C,Kearney L,Buckle VJ,Doggett NA,Flint J,Higgs DR

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PGAP6 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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