PYGB cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PYGB
Entrez Gene ID	5834
Full Name	glycogen phosphorylase B
Synonyms	GPBB
General protein information	Preferred Names glycogen phosphorylase B Names glycogen phosphorylase, brain form phosphorylase, glycogen; brain
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20p11.21
Summary	The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation. [provided by RefSeq, Jul 2008].
Disorder MIM:	138550

mRNA and Protein(s)

mRNA	Protein	Name
NM_002862.3	NP_002853.2	glycogen phosphorylase, brain form
NM_002862.4	NP_002853.2	glycogen phosphorylase, brain form

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Pygb	NP_037320.1
Danio rerio (zebrafish)	pygb	NP_997974.2
Pan troglodytes (chimpanzee)	PYGB	XP_525293.4
Mus musculus (house mouse)	Pygb	NP_722476.1
Bos taurus (cattle)	PYGB	NP_001030347.1
Gallus gallus (chicken)	PYGB	NP_001026205.1
	PYGB	XP_001100555.1
Xenopus tropicalis (tropical clawed frog)	pygm	NP_001001904.1
Homo sapiens (human)	PYGB	NP_002853.2
Canis lupus familiaris (dog)	PYGB	XP_003433179.1

Gene Ontology
Bibliography

Related articles in PubMed

New biomarker for acute ischaemic stroke: plasma glycogen phosphorylase isoenzyme BB.
Park KY, Ay I, Avery R, Caceres JA, Siket MS, Pontes-Neto OM, Zheng H, Rost NS, Furie KL, Sorensen AG, Koroshetz WJ, Ay H
Journal of neurology, neurosurgery, and psychiatry(2017 Oct)

Molecular Mechanisms of Allosteric Inhibition of Brain Glycogen Phosphorylase by Neurotoxic Dithiocarbamate Chemicals.
Mathieu C, Bui LC, Petit E, Haddad I, Agbulut O, Vinh J, Dupret JM, Rodrigues-Lima F
The Journal of biological chemistry292(5)1603-1612(2017 02)

An Isozyme-specific Redox Switch in Human Brain Glycogen Phosphorylase Modulates Its Allosteric Activation by AMP.
Mathieu C, Duval R, Cocaign A, Petit E, Bui LC, Haddad I, Vinh J, Etchebest C, Dupret JM, Rodrigues-Lima F
The Journal of biological chemistry291(46)23842-23853(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Using cysteine chemical labeling, mass spectrometry, and site-directed mutagenesis approaches, we show that thiram (and certain of its metabolites) alters the activity of bGP through the formation of an intramolecular disulfide bond (Cys(318)-Cys(326)), known to act as a redox switch that precludes the allosteric activation of bGP by AMP.
Title: Molecular Mechanisms of Allosteric Inhibition of Brain Glycogen Phosphorylase by Neurotoxic Dithiocarbamate Chemicals.

disulfide bond acts as a redox switch that precludes the allosteric activation of the enzyme by AMP without affecting its activation by phosphorylation. This unique regulatory feature of bGP sheds new light on the isoform-specific regulation of glycogen phosphorylase and glycogen metabolism.
Title: An Isozyme-specific Redox Switch in Human Brain Glycogen Phosphorylase Modulates Its Allosteric Activation by AMP.

the bGP structures reveal molecular features unique to the brain isozyme that provide a deeper understanding of the differences in the activation properties of these allosteric enzymes by the allosteric effector AMP.
Title: Insights into Brain Glycogen Metabolism: THE STRUCTURE OF HUMAN BRAIN GLYCOGEN PHOSPHORYLASE.

GPBB is a valuable biological marker to predict the prognosis in patient with acute coronary syndrome.
Title: Prognostic information of glycogen phosphorylase isoenzyme BB in patients with suspected acute coronary syndrome.

H-FABP and GPBB can contribute to early acute myocardial infarction diagnosis and can distinguish acute myocardial infarction from acute coronary syndrome
Title: Diagnostic accuracy of heart fatty acid binding protein (H-FABP) and glycogen phosphorylase isoenzyme BB (GPBB) in diagnosis of acute myocardial infarction in patients with acute coronary syndrome.

The following PYGB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PYGB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19479	NM_002862.4 Latest version!	Homo sapiens glycogen phosphorylase B (PYGB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu19479	NM_002862.3	Homo sapiens glycogen phosphorylase B (PYGB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PYGB ( NM_002862.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002853.2 Homo sapiens glycogen phosphorylase B (PYGB), mRNA.

Price & Availability↑

CloneID	OHu19479
Clone ID Related Accession (Same CDS sequence)	NM_002862.3 , NM_002862.4
Accession Version	NM_002862.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	glycogen phosphorylase, brain form
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC017045.2, BQ433140.1 and U47025.1. On Sep 11, 2003 this sequence version replaced NM_002862.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.139537.1, SRR1803615.66518.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGAAGC CGCTGACGGA CAGCGAGAAG CGGAAGCAGA TCAGCGTGCG CGGCCTGGCG 
GGGCTAGGCG ACGTGGCCGA GGTGCGGAAG AGCTTCAACC GGCACTTGCA CTTCACGCTG 
GTCAAGGACC GCAATGTGGC CACGCCCCGC GACTACTTCT TCGCGCTGGC GCACACGGTG 
CGCGACCACC TCGTGGGCCG CTGGATCCGC ACGCAGCAGC ACTACTACGA GCGCGACCCC 
AAGCGCATTT ATTATCTTTC CCTGGAATTC TACATGGGTC GCACGCTGCA GAACACGATG 
GTGAACCTGG GCCTTCAGAA TGCCTGCGAT GAAGCCATCT ATCAGTTGGG GTTAGACTTG 
GAGGAACTCG AGGAGATAGA AGAAGATGCT GGCCTTGGGA ATGGAGGCCT GGGGAGGCTG 
GCAGCGTGTT TCCTTGACTC AATGGCTACC TTGGGCCTGG CAGCATACGG CTATGGAATC 
CGCTATGAAT TTGGGATTTT TAACCAGAAG ATTGTCAATG GCTGGCAGGT AGAGGAGGCC 
GATGACTGGC TGCGCTACGG CAACCCCTGG GAGAAAGCGC GGCCTGAGTA TATGCTTCCC 
GTGCACTTCT ACGGACGCGT GGAGCACACC CCCGACGGCG TGAAGTGGCT GGACACACAG 
GTGGTGCTGG CCATGCCCTA CGACACCCCA GTGCCCGGCT ACAAGAACAA CACCGTCAAC 
ACCATGCGGC TGTGGTCCGC CAAGGCTCCC AACGACTTCA AGCTGCAGGA CTTCAACGTG 
GGAGACTACA TCGAGGCGGT CCTGGACCGG AACTTGGCTG AGAACATCTC CAGGGTCCTG 
TATCCAAATG ATAACTTCTT TGAGGGGAAG GAGCTGCGGC TGAAGCAGGA GTACTTCGTG 
GTGGCCGCCA CGCTCCAGGA CATCATCCGC CGCTTCAAGT CGTCCAAGTT CGGCTGCCGG 
GACCCTGTGA GAACCTGTTT CGAGACGTTC CCAGACAAGG TGGCCATCCA GCTGAACGAC 
ACCCACCCCG CCCTCTCCAT CCCTGAGCTC ATGCGGATCC TGGTGGACGT GGAGAAGGTG 
GACTGGGACA AGGCCTGGGA AATCACGAAG AAGACCTGTG CATACACCAA CCACACTGTG 
CTGCCTGAGG CCTTGGAGCG CTGGCCCGTG TCCATGTTTG AGAAGCTGCT GCCGCGGCAC 
CTGGAGATAA TCTATGCCAT CAACCAGCGG CACCTGGACC ACGTGGCCGC GCTGTTTCCC 
GGCGATGTGG ACCGCCTGCG CAGGATGTCT GTGATCGAGG AGGGGGACTG CAAGCGGATC 
AACATGGCCC ACCTGTGTGT GATTGGGTCC CATGCTGTCA ATGGTGTGGC GAGGATCCAC 
TCGGAGATCG TGAAACAGTC GGTCTTTAAG GATTTTTATG AACTGGAGCC AGAGAAGTTC 
CAGAATAAGA CCAATGGCAT CACCCCCCGC CGGTGGCTGC TGCTGTGCAA CCCGGGGCTG 
GCCGATACCA TCGTGGAGAA AATTGGGGAG GAGTTCCTGA CTGACCTGAG CCAGCTGAAG 
AAGCTGCTGC CGCTGGTCAG TGACGAGGTG TTCATCAGGG ACGTGGCCAA GGTCAAACAG 
GAGAACAAGC TCAAGTTCTC GGCCTTCCTG GAGAAGGAGT ACAAGGTGAA GATCAACCCC 
TCCTCCATGT TCGATGTGCA TGTGAAGAGG ATCCACGAGT ACAAGCGGCA GCTGCTCAAC 
TGCCTGCACG TCGTCACCCT GTACAATCGA ATCAAGAGAG ACCCGGCCAA GGCTTTTGTG 
CCCAGGACTG TTATGATTGG GGGCAAGGCA GCGCCCGGTT ACCACATGGC CAAGCTGATC 
ATCAAGTTGG TCACCTCCAT CGGCGACGTC GTCAATCATG ACCCAGTTGT GGGTGACAGG 
TTGAAAGTGA TCTTCCTGGA GAACTACCGT GTGTCCTTGG CTGAGAAAGT GATCCCGGCC 
GCTGATCTGT CGCAGCAGAT CTCCACTGCA GGCACCGAGG CCTCAGGCAC AGGCAACATG 
AAGTTCATGC TCAACGGGGC CCTCACCATC GGCACCATGG ACGGCGCCAA CGTGGAGATG 
GCCGAGGAGG CCGGGGCCGA GAACCTCTTC ATCTTCGGCC TGCGGGTGGA GGATGTCGAG 
GCCTTGGACC GGAAAGGGTA CAATGCCAGG GAGTACTACG ACCACCTGCC CGAGCTGAAG 
CAGGCCGTGG ACCAGATCAG CAGTGGCTTT TTTTCTCCCA AGGAGCCAGA CTGCTTCAAG 
GACATCGTGA ACATGCTGAT GCACCATGAC AGGTTCAAGG TGTTTGCAGA CTATGAAGCC 
TACATGCAGT GCCAGGCACA GGTGGACCAG CTGTACCGGA ACCCCAAGGA GTGGACCAAG 
AAGGTCATCA GGAACATCGC CTGCTCGGGC AAGTTCTCCA GTGACCGGAC CATCACGGAG 
TATGCACGGG AGATCTGGGG TGTGGAGCCC TCCGACCTGC AGATCCCGCC CCCCAACATC 
CCCCGGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002853.2
CDS	110..2641
Translation	MAKPLTDSEKRKQISVRGLAGLGDVAEVRKSFNRHLHFTLVKDRNVATPRDYFFALAHTVRDHLVGRWIRTQQHYYERDPKRIYYLSLEFYMGRTLQNTMVNLGLQNACDEAIYQLGLDLEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEFGIFNQKIVNGWQVEEADDWLRYGNPWEKARPEYMLPVHFYGRVEHTPDGVKWLDTQVVLAMPYDTPVPGYKNNTVNTMRLWSAKAPNDFKLQDFNVGDYIEAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGCRDPVRTCFETFPDKVAIQLNDTHPALSIPELMRILVDVEKVDWDKAWEITKKTCAYTNHTVLPEALERWPVSMFEKLLPRHLEIIYAINQRHLDHVAALFPGDVDRLRRMSVIEEGDCKRINMAHLCVIGSHAVNGVARIHSEIVKQSVFKDFYELEPEKFQNKTNGITPRRWLLLCNPGLADTIVEKIGEEFLTDLSQLKKLLPLVSDEVFIRDVAKVKQENKLKFSAFLEKEYKVKINPSSMFDVHVKRIHEYKRQLLNCLHVVTLYNRIKRDPAKAFVPRTVMIGGKAAPGYHMAKLIIKLVTSIGDVVNHDPVVGDRLKVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGAENLFIFGLRVEDVEALDRKGYNAREYYDHLPELKQAVDQISSGFFSPKEPDCFKDIVNMLMHHDRFKVFADYEAYMQCQAQVDQLYRNPKEWTKKVIRNIACSGKFSSDRTITEYAREIWGVEPSDLQIPPPNIPRD

Target ORF information:

RefSeq Version	NM_002862.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glycogen phosphorylase B (PYGB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002862.3

ORF Insert Sequence:

ATGGCGAAGC CGCTGACGGA CAGCGAGAAG CGGAAGCAGA TCAGCGTGCG CGGCCTGGCG 
GGGCTAGGCG ACGTGGCCGA GGTGCGGAAG AGCTTCAACC GGCACTTGCA CTTCACGCTG 
GTCAAGGACC GCAATGTGGC CACGCCCCGC GACTACTTCT TCGCGCTGGC GCACACGGTG 
CGCGACCACC TCGTGGGCCG CTGGATCCGC ACGCAGCAGC ACTACTACGA GCGCGACCCC 
AAGCGCATTT ATTATCTTTC CCTGGAATTC TACATGGGTC GCACGCTGCA GAACACGATG 
GTGAACCTGG GCCTTCAGAA TGCCTGCGAT GAAGCCATCT ATCAGTTGGG GTTAGACTTG 
GAGGAACTCG AGGAGATAGA AGAAGATGCT GGCCTTGGGA ATGGAGGCCT GGGGAGGCTG 
GCAGCGTGTT TCCTTGACTC AATGGCTACC TTGGGCCTGG CAGCATACGG CTATGGAATC 
CGCTATGAAT TTGGGATTTT TAACCAGAAG ATTGTCAATG GCTGGCAGGT AGAGGAGGCC 
GATGACTGGC TGCGCTACGG CAACCCCTGG GAGAAAGCGC GGCCTGAGTA TATGCTTCCC 
GTGCACTTCT ACGGACGCGT GGAGCACACC CCCGACGGCG TGAAGTGGCT GGACACACAG 
GTGGTGCTGG CCATGCCCTA CGACACCCCA GTGCCCGGCT ACAAGAACAA CACCGTCAAC 
ACCATGCGGC TGTGGTCCGC CAAGGCTCCC AACGACTTCA AGCTGCAGGA CTTCAACGTG 
GGAGACTACA TCGAGGCGGT CCTGGACCGG AACTTGGCTG AGAACATCTC CAGGGTCCTG 
TATCCAAATG ATAACTTCTT TGAGGGGAAG GAGCTGCGGC TGAAGCAGGA GTACTTCGTG 
GTGGCCGCCA CGCTCCAGGA CATCATCCGC CGCTTCAAGT CGTCCAAGTT CGGCTGCCGG 
GACCCTGTGA GAACCTGTTT CGAGACGTTC CCAGACAAGG TGGCCATCCA GCTGAACGAC 
ACCCACCCCG CCCTCTCCAT CCCTGAGCTC ATGCGGATCC TGGTGGACGT GGAGAAGGTG 
GACTGGGACA AGGCCTGGGA AATCACGAAG AAGACCTGTG CATACACCAA CCACACTGTG 
CTGCCTGAGG CCTTGGAGCG CTGGCCCGTG TCCATGTTTG AGAAGCTGCT GCCGCGGCAC 
CTGGAGATAA TCTATGCCAT CAACCAGCGG CACCTGGACC ACGTGGCCGC GCTGTTTCCC 
GGCGATGTGG ACCGCCTGCG CAGGATGTCT GTGATCGAGG AGGGGGACTG CAAGCGGATC 
AACATGGCCC ACCTGTGTGT GATTGGGTCC CATGCTGTCA ATGGTGTGGC GAGGATCCAC 
TCGGAGATCG TGAAACAGTC GGTCTTTAAG GATTTTTATG AACTGGAGCC AGAGAAGTTC 
CAGAATAAGA CCAATGGCAT CACCCCCCGC CGGTGGCTGC TGCTGTGCAA CCCGGGGCTG 
GCCGATACCA TCGTGGAGAA AATTGGGGAG GAGTTCCTGA CTGACCTGAG CCAGCTGAAG 
AAGCTGCTGC CGCTGGTCAG TGACGAGGTG TTCATCAGGG ACGTGGCCAA GGTCAAACAG 
GAGAACAAGC TCAAGTTCTC GGCCTTCCTG GAGAAGGAGT ACAAGGTGAA GATCAACCCC 
TCCTCCATGT TCGATGTGCA TGTGAAGAGG ATCCACGAGT ACAAGCGGCA GCTGCTCAAC 
TGCCTGCACG TCGTCACCCT GTACAATCGA ATCAAGAGAG ACCCGGCCAA GGCTTTTGTG 
CCCAGGACTG TTATGATTGG GGGCAAGGCA GCGCCCGGTT ACCACATGGC CAAGCTGATC 
ATCAAGTTGG TCACCTCCAT CGGCGACGTC GTCAATCATG ACCCAGTTGT GGGTGACAGG 
TTGAAAGTGA TCTTCCTGGA GAACTACCGT GTGTCCTTGG CTGAGAAAGT GATCCCGGCC 
GCTGATCTGT CGCAGCAGAT CTCCACTGCA GGCACCGAGG CCTCAGGCAC AGGCAACATG 
AAGTTCATGC TCAACGGGGC CCTCACCATC GGCACCATGG ACGGCGCCAA CGTGGAGATG 
GCCGAGGAGG CCGGGGCCGA GAACCTCTTC ATCTTCGGCC TGCGGGTGGA GGATGTCGAG 
GCCTTGGACC GGAAAGGGTA CAATGCCAGG GAGTACTACG ACCACCTGCC CGAGCTGAAG 
CAGGCCGTGG ACCAGATCAG CAGTGGCTTT TTTTCTCCCA AGGAGCCAGA CTGCTTCAAG 
GACATCGTGA ACATGCTGAT GCACCATGAC AGGTTCAAGG TGTTTGCAGA CTATGAAGCC 
TACATGCAGT GCCAGGCACA GGTGGACCAG CTGTACCGGA ACCCCAAGGA GTGGACCAAG 
AAGGTCATCA GGAACATCGC CTGCTCGGGC AAGTTCTCCA GTGACCGGAC CATCACGGAG 
TATGCACGGG AGATCTGGGG TGTGGAGCCC TCCGACCTGC AGATCCCGCC CCCCAACATC 
CCCCGGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PYGB ( NM_002862.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002853.2 Homo sapiens glycogen phosphorylase B (PYGB), mRNA.

Price & Availability↑

CloneID	OHu19479
Clone ID Related Accession (Same CDS sequence)	NM_002862.3 , NM_002862.4
Accession Version	NM_002862.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	glycogen phosphorylase, brain form
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121772.19. On Nov 26, 2018 this sequence version replaced NM_002862.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.211977.1, SRR1660807.231969.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000216962.9/ ENSP00000216962.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGAAGC CGCTGACGGA CAGCGAGAAG CGGAAGCAGA TCAGCGTGCG CGGCCTGGCG 
GGGCTAGGCG ACGTGGCCGA GGTGCGGAAG AGCTTCAACC GGCACTTGCA CTTCACGCTG 
GTCAAGGACC GCAATGTGGC CACGCCCCGC GACTACTTCT TCGCGCTGGC GCACACGGTG 
CGCGACCACC TCGTGGGCCG CTGGATCCGC ACGCAGCAGC ACTACTACGA GCGCGACCCC 
AAGCGCATTT ATTATCTTTC CCTGGAATTC TACATGGGTC GCACGCTGCA GAACACGATG 
GTGAACCTGG GCCTTCAGAA TGCCTGCGAT GAAGCCATCT ATCAGTTGGG GTTAGACTTG 
GAGGAACTCG AGGAGATAGA AGAAGATGCT GGCCTTGGGA ATGGAGGCCT GGGGAGGCTG 
GCAGCGTGTT TCCTTGACTC AATGGCTACC TTGGGCCTGG CAGCATACGG CTATGGAATC 
CGCTATGAAT TTGGGATTTT TAACCAGAAG ATTGTCAATG GCTGGCAGGT AGAGGAGGCC 
GATGACTGGC TGCGCTACGG CAACCCCTGG GAGAAAGCGC GGCCTGAGTA TATGCTTCCC 
GTGCACTTCT ACGGACGCGT GGAGCACACC CCCGACGGCG TGAAGTGGCT GGACACACAG 
GTGGTGCTGG CCATGCCCTA CGACACCCCA GTGCCCGGCT ACAAGAACAA CACCGTCAAC 
ACCATGCGGC TGTGGTCCGC CAAGGCTCCC AACGACTTCA AGCTGCAGGA CTTCAACGTG 
GGAGACTACA TCGAGGCGGT CCTGGACCGG AACTTGGCTG AGAACATCTC CAGGGTCCTG 
TATCCAAATG ATAACTTCTT TGAGGGGAAG GAGCTGCGGC TGAAGCAGGA GTACTTCGTG 
GTGGCCGCCA CGCTCCAGGA CATCATCCGC CGCTTCAAGT CGTCCAAGTT CGGCTGCCGG 
GACCCTGTGA GAACCTGTTT CGAGACGTTC CCAGACAAGG TGGCCATCCA GCTGAACGAC 
ACCCACCCCG CCCTCTCCAT CCCTGAGCTC ATGCGGATCC TGGTGGACGT GGAGAAGGTG 
GACTGGGACA AGGCCTGGGA AATCACGAAG AAGACCTGTG CATACACCAA CCACACTGTG 
CTGCCTGAGG CCTTGGAGCG CTGGCCCGTG TCCATGTTTG AGAAGCTGCT GCCGCGGCAC 
CTGGAGATAA TCTATGCCAT CAACCAGCGG CACCTGGACC ACGTGGCCGC GCTGTTTCCC 
GGCGATGTGG ACCGCCTGCG CAGGATGTCT GTGATCGAGG AGGGGGACTG CAAGCGGATC 
AACATGGCCC ACCTGTGTGT GATTGGGTCC CATGCTGTCA ATGGTGTGGC GAGGATCCAC 
TCGGAGATCG TGAAACAGTC GGTCTTTAAG GATTTTTATG AACTGGAGCC AGAGAAGTTC 
CAGAATAAGA CCAATGGCAT CACCCCCCGC CGGTGGCTGC TGCTGTGCAA CCCGGGGCTG 
GCCGATACCA TCGTGGAGAA AATTGGGGAG GAGTTCCTGA CTGACCTGAG CCAGCTGAAG 
AAGCTGCTGC CGCTGGTCAG TGACGAGGTG TTCATCAGGG ACGTGGCCAA GGTCAAACAG 
GAGAACAAGC TCAAGTTCTC GGCCTTCCTG GAGAAGGAGT ACAAGGTGAA GATCAACCCC 
TCCTCCATGT TCGATGTGCA TGTGAAGAGG ATCCACGAGT ACAAGCGGCA GCTGCTCAAC 
TGCCTGCACG TCGTCACCCT GTACAATCGA ATCAAGAGAG ACCCGGCCAA GGCTTTTGTG 
CCCAGGACTG TTATGATTGG GGGCAAGGCA GCGCCCGGTT ACCACATGGC CAAGCTGATC 
ATCAAGTTGG TCACCTCCAT CGGCGACGTC GTCAATCATG ACCCAGTTGT GGGTGACAGG 
TTGAAAGTGA TCTTCCTGGA GAACTACCGT GTGTCCTTGG CTGAGAAAGT GATCCCGGCC 
GCTGATCTGT CGCAGCAGAT CTCCACTGCA GGCACCGAGG CCTCAGGCAC AGGCAACATG 
AAGTTCATGC TCAACGGGGC CCTCACCATC GGCACCATGG ACGGCGCCAA CGTGGAGATG 
GCCGAGGAGG CCGGGGCCGA GAACCTCTTC ATCTTCGGCC TGCGGGTGGA GGATGTCGAG 
GCCTTGGACC GGAAAGGGTA CAATGCCAGG GAGTACTACG ACCACCTGCC CGAGCTGAAG 
CAGGCCGTGG ACCAGATCAG CAGTGGCTTT TTTTCTCCCA AGGAGCCAGA CTGCTTCAAG 
GACATCGTGA ACATGCTGAT GCACCATGAC AGGTTCAAGG TGTTTGCAGA CTATGAAGCC 
TACATGCAGT GCCAGGCACA GGTGGACCAG CTGTACCGGA ACCCCAAGGA GTGGACCAAG 
AAGGTCATCA GGAACATCGC CTGCTCGGGC AAGTTCTCCA GTGACCGGAC CATCACGGAG 
TATGCACGGG AGATCTGGGG TGTGGAGCCC TCCGACCTGC AGATCCCGCC CCCCAACATC 
CCCCGGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002853.2
CDS	95..2626
Translation	MAKPLTDSEKRKQISVRGLAGLGDVAEVRKSFNRHLHFTLVKDRNVATPRDYFFALAHTVRDHLVGRWIRTQQHYYERDPKRIYYLSLEFYMGRTLQNTMVNLGLQNACDEAIYQLGLDLEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEFGIFNQKIVNGWQVEEADDWLRYGNPWEKARPEYMLPVHFYGRVEHTPDGVKWLDTQVVLAMPYDTPVPGYKNNTVNTMRLWSAKAPNDFKLQDFNVGDYIEAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGCRDPVRTCFETFPDKVAIQLNDTHPALSIPELMRILVDVEKVDWDKAWEITKKTCAYTNHTVLPEALERWPVSMFEKLLPRHLEIIYAINQRHLDHVAALFPGDVDRLRRMSVIEEGDCKRINMAHLCVIGSHAVNGVARIHSEIVKQSVFKDFYELEPEKFQNKTNGITPRRWLLLCNPGLADTIVEKIGEEFLTDLSQLKKLLPLVSDEVFIRDVAKVKQENKLKFSAFLEKEYKVKINPSSMFDVHVKRIHEYKRQLLNCLHVVTLYNRIKRDPAKAFVPRTVMIGGKAAPGYHMAKLIIKLVTSIGDVVNHDPVVGDRLKVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGAENLFIFGLRVEDVEALDRKGYNAREYYDHLPELKQAVDQISSGFFSPKEPDCFKDIVNMLMHHDRFKVFADYEAYMQCQAQVDQLYRNPKEWTKKVIRNIACSGKFSSDRTITEYAREIWGVEPSDLQIPPPNIPRD

Target ORF information:

RefSeq Version	NM_002862.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glycogen phosphorylase B (PYGB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002862.4

ORF Insert Sequence:

ATGGCGAAGC CGCTGACGGA CAGCGAGAAG CGGAAGCAGA TCAGCGTGCG CGGCCTGGCG 
GGGCTAGGCG ACGTGGCCGA GGTGCGGAAG AGCTTCAACC GGCACTTGCA CTTCACGCTG 
GTCAAGGACC GCAATGTGGC CACGCCCCGC GACTACTTCT TCGCGCTGGC GCACACGGTG 
CGCGACCACC TCGTGGGCCG CTGGATCCGC ACGCAGCAGC ACTACTACGA GCGCGACCCC 
AAGCGCATTT ATTATCTTTC CCTGGAATTC TACATGGGTC GCACGCTGCA GAACACGATG 
GTGAACCTGG GCCTTCAGAA TGCCTGCGAT GAAGCCATCT ATCAGTTGGG GTTAGACTTG 
GAGGAACTCG AGGAGATAGA AGAAGATGCT GGCCTTGGGA ATGGAGGCCT GGGGAGGCTG 
GCAGCGTGTT TCCTTGACTC AATGGCTACC TTGGGCCTGG CAGCATACGG CTATGGAATC 
CGCTATGAAT TTGGGATTTT TAACCAGAAG ATTGTCAATG GCTGGCAGGT AGAGGAGGCC 
GATGACTGGC TGCGCTACGG CAACCCCTGG GAGAAAGCGC GGCCTGAGTA TATGCTTCCC 
GTGCACTTCT ACGGACGCGT GGAGCACACC CCCGACGGCG TGAAGTGGCT GGACACACAG 
GTGGTGCTGG CCATGCCCTA CGACACCCCA GTGCCCGGCT ACAAGAACAA CACCGTCAAC 
ACCATGCGGC TGTGGTCCGC CAAGGCTCCC AACGACTTCA AGCTGCAGGA CTTCAACGTG 
GGAGACTACA TCGAGGCGGT CCTGGACCGG AACTTGGCTG AGAACATCTC CAGGGTCCTG 
TATCCAAATG ATAACTTCTT TGAGGGGAAG GAGCTGCGGC TGAAGCAGGA GTACTTCGTG 
GTGGCCGCCA CGCTCCAGGA CATCATCCGC CGCTTCAAGT CGTCCAAGTT CGGCTGCCGG 
GACCCTGTGA GAACCTGTTT CGAGACGTTC CCAGACAAGG TGGCCATCCA GCTGAACGAC 
ACCCACCCCG CCCTCTCCAT CCCTGAGCTC ATGCGGATCC TGGTGGACGT GGAGAAGGTG 
GACTGGGACA AGGCCTGGGA AATCACGAAG AAGACCTGTG CATACACCAA CCACACTGTG 
CTGCCTGAGG CCTTGGAGCG CTGGCCCGTG TCCATGTTTG AGAAGCTGCT GCCGCGGCAC 
CTGGAGATAA TCTATGCCAT CAACCAGCGG CACCTGGACC ACGTGGCCGC GCTGTTTCCC 
GGCGATGTGG ACCGCCTGCG CAGGATGTCT GTGATCGAGG AGGGGGACTG CAAGCGGATC 
AACATGGCCC ACCTGTGTGT GATTGGGTCC CATGCTGTCA ATGGTGTGGC GAGGATCCAC 
TCGGAGATCG TGAAACAGTC GGTCTTTAAG GATTTTTATG AACTGGAGCC AGAGAAGTTC 
CAGAATAAGA CCAATGGCAT CACCCCCCGC CGGTGGCTGC TGCTGTGCAA CCCGGGGCTG 
GCCGATACCA TCGTGGAGAA AATTGGGGAG GAGTTCCTGA CTGACCTGAG CCAGCTGAAG 
AAGCTGCTGC CGCTGGTCAG TGACGAGGTG TTCATCAGGG ACGTGGCCAA GGTCAAACAG 
GAGAACAAGC TCAAGTTCTC GGCCTTCCTG GAGAAGGAGT ACAAGGTGAA GATCAACCCC 
TCCTCCATGT TCGATGTGCA TGTGAAGAGG ATCCACGAGT ACAAGCGGCA GCTGCTCAAC 
TGCCTGCACG TCGTCACCCT GTACAATCGA ATCAAGAGAG ACCCGGCCAA GGCTTTTGTG 
CCCAGGACTG TTATGATTGG GGGCAAGGCA GCGCCCGGTT ACCACATGGC CAAGCTGATC 
ATCAAGTTGG TCACCTCCAT CGGCGACGTC GTCAATCATG ACCCAGTTGT GGGTGACAGG 
TTGAAAGTGA TCTTCCTGGA GAACTACCGT GTGTCCTTGG CTGAGAAAGT GATCCCGGCC 
GCTGATCTGT CGCAGCAGAT CTCCACTGCA GGCACCGAGG CCTCAGGCAC AGGCAACATG 
AAGTTCATGC TCAACGGGGC CCTCACCATC GGCACCATGG ACGGCGCCAA CGTGGAGATG 
GCCGAGGAGG CCGGGGCCGA GAACCTCTTC ATCTTCGGCC TGCGGGTGGA GGATGTCGAG 
GCCTTGGACC GGAAAGGGTA CAATGCCAGG GAGTACTACG ACCACCTGCC CGAGCTGAAG 
CAGGCCGTGG ACCAGATCAG CAGTGGCTTT TTTTCTCCCA AGGAGCCAGA CTGCTTCAAG 
GACATCGTGA ACATGCTGAT GCACCATGAC AGGTTCAAGG TGTTTGCAGA CTATGAAGCC 
TACATGCAGT GCCAGGCACA GGTGGACCAG CTGTACCGGA ACCCCAAGGA GTGGACCAAG 
AAGGTCATCA GGAACATCGC CTGCTCGGGC AAGTTCTCCA GTGACCGGAC CATCACGGAG 
TATGCACGGG AGATCTGGGG TGTGGAGCCC TCCGACCTGC AGATCCCGCC CCCCAACATC 
CCCCGGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

New biomarker for acute ischaemic stroke: plasma glycogen phosphorylase isoenzyme BB.
Journal of neurology, neurosurgery, and psychiatry(2017 Oct)
Park KY,Ay I,Avery R,Caceres JA,Siket MS,Pontes-Neto OM,Zheng H,Rost NS,Furie KL,Sorensen AG,Koroshetz WJ,Ay H

Molecular Mechanisms of Allosteric Inhibition of Brain Glycogen Phosphorylase by Neurotoxic Dithiocarbamate Chemicals.
The Journal of biological chemistry292(5)1603-1612(2017 02)
Mathieu C,Bui LC,Petit E,Haddad I,Agbulut O,Vinh J,Dupret JM,Rodrigues-Lima F

An Isozyme-specific Redox Switch in Human Brain Glycogen Phosphorylase Modulates Its Allosteric Activation by AMP.
The Journal of biological chemistry291(46)23842-23853(2016 Nov)
Mathieu C,Duval R,Cocaign A,Petit E,Bui LC,Haddad I,Vinh J,Etchebest C,Dupret JM,Rodrigues-Lima F

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PYGB cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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