HAMP cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HAMP
Entrez Gene ID	57817
Full Name	hepcidin antimicrobial peptide
Synonyms	HEPC,HFE2B,LEAP1,PLTR
General protein information	Preferred Names hepcidin antimicrobial peptide Names hepcidin liver-expressed antimicrobial peptide 1 putative liver tumor regressor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.12
Summary	The product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity against bacteria and fungi. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure. [provided by RefSeq, Oct 2014].
Disorder MIM:	606464
Disorder Html:	Hemochromatosis, type 2B, 613313 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_021175.3	NP_066998.1	hepcidin preproprotein
NM_021175.4	NP_066998.1	hepcidin preproprotein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HAMP	NP_001103163.1
	HAMP	XP_001094273.1
Canis lupus familiaris (dog)	HAMP	NP_001007141.1
Homo sapiens (human)	HAMP	NP_066998.1
Bos taurus (cattle)	HAMP	NP_001107980.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of novel bone morphogenetic protein- responsive elements in a hepcidin promoter.
Kanamori Y, Murakami M, Matsui T, Funaba M
FEBS letters591(23)3895-3905(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data (including data from studies using knockout mice) suggest that MT2/TMPRSS6 suppresses hepcidin expression in hepatocytes independently of HJV; MT2/TMPRSS6 cleaves ALK2, ALK3, ACTRIIA, BMPR2, HFE, and, to a lesser extent, HJV and TFR2; thus, MT2/TMPRSS6 suppresses hepcidin expression by cleaving multiple components of the hepcidin induction pathway. (MT2/TMPRSS6 = matriptase-2; HJV = hemojuvelin)
Title: Matriptase-2 suppresses hepcidin expression by cleaving multiple components of the hepcidin induction pathway.

During regulation of hepcidin synthesis, multiple promoter elements in the HAMP gene respond to variable signaling pathways corresponding to different extracellular situations.
Title: Reciprocal regulation between hepcidin and erythropoiesis and its therapeutic application in erythroid disorders.

Our findings indicate that the HAMP-P -582A>G polymorphism (rs10421768) is associated with susceptibility to extrapulmonary TB, but not pulmonary TB. CD14+ monocytes from individuals with the rs10421768 GG genotype secreted significantly less hepcidin in response to M. tuberculosis lipoarabinomannan compared with cells from individuals with either the AA or AG genotypes.
Title: Association of Single-Nucleotide Polymorphism in the Hepcidin Promoter Gene with Susceptibility to Extrapulmonary Tuberculosis.

Data suggest that proinflammatory cytokine interleukin-1beta (IL1B) up-regulates hepcidin expression in hepatocytes; inflammation induces IL1B production in Kupffer cells and hepatocytes; IL1B up-regulates CEBPD (CCAAT/enhancer binding protein delta) expression in hepatocytes; up-regulation of CEBPD expression up-regulates hepcidin transcription.
Title: Interleukin-1? (IL-1?) transcriptionally activates hepcidin by inducing CCAAT enhancer-binding protein ? (C/EBP?) expression in hepatocytes.

The hepcidin plays a role in the pathogenesis and progression of kidney injury via modulation of iron-mediated oxidant injury.
Title: Role of Hepcidin-25 in Chronic Kidney Disease: Anemia and Beyond.

The following HAMP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HAMP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25594	NM_021175.4 Latest version!	Homo sapiens hepcidin antimicrobial peptide (HAMP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu25594	NM_021175.3	Homo sapiens hepcidin antimicrobial peptide (HAMP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HAMP ( NM_021175.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_066998.1 Homo sapiens hepcidin antimicrobial peptide (HAMP), mRNA.

Price & Availability↑

CloneID	OHu25594
Clone ID Related Accession (Same CDS sequence)	NM_021175.3 , NM_021175.4
Accession Version	NM_021175.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	hepcidin preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP306149.1, AD000684.1, AF309489.1 and AY358669.1. This sequence is a reference standard in the RefSeqGene project. On Jul 17, 2015 this sequence version replaced NM_021175.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX089371.1, BP306149.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## Protein has antimicrobial activity :: PMID: 11034317 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCACTGA GCTCCCAGAT CTGGGCCGCT TGCCTCCTGC TCCTCCTCCT CCTCGCCAGC 
CTGACCAGTG GCTCTGTTTT CCCACAACAG ACGGGACAAC TTGCAGAGCT GCAACCCCAG 
GACAGAGCTG GAGCCAGGGC CAGCTGGATG CCCATGTTCC AGAGGCGAAG GAGGCGAGAC 
ACCCACTTCC CCATCTGCAT TTTCTGCTGC GGCTGCTGTC ATCGATCAAA GTGTGGGATG 
TGCTGCAAGA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066998.1
CDS	233..487
Translation	MALSSQIWAACLLLLLLLASLTSGSVFPQQTGQLAELQPQDRAGARASWMPMFQRRRRRDTHFPICIFCCGCCHRSKCGMCCKT

Target ORF information:

RefSeq Version	NM_021175.3
Organism	Homo sapiens(human)
Definition	Homo sapiens hepcidin antimicrobial peptide (HAMP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021175.3

ORF Insert Sequence:

ATGGCACTGA GCTCCCAGAT CTGGGCCGCT TGCCTCCTGC TCCTCCTCCT CCTCGCCAGC 
CTGACCAGTG GCTCTGTTTT CCCACAACAG ACGGGACAAC TTGCAGAGCT GCAACCCCAG 
GACAGAGCTG GAGCCAGGGC CAGCTGGATG CCCATGTTCC AGAGGCGAAG GAGGCGAGAC 
ACCCACTTCC CCATCTGCAT TTTCTGCTGC GGCTGCTGTC ATCGATCAAA GTGTGGGATG 
TGCTGCAAGA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HAMP ( NM_021175.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_066998.1 Homo sapiens hepcidin antimicrobial peptide (HAMP), mRNA.

Price & Availability↑

CloneID	OHu25594
Clone ID Related Accession (Same CDS sequence)	NM_021175.3 , NM_021175.4
Accession Version	NM_021175.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Homo sapiens(human)
Product	hepcidin preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF309489.1 and AY358669.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_021175.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC020612.1, BP346863.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000222304.5/ ENSP00000222304.2 Protein has antimicrobial activity :: PMID: 11034317 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCACTGA GCTCCCAGAT CTGGGCCGCT TGCCTCCTGC TCCTCCTCCT CCTCGCCAGC 
CTGACCAGTG GCTCTGTTTT CCCACAACAG ACGGGACAAC TTGCAGAGCT GCAACCCCAG 
GACAGAGCTG GAGCCAGGGC CAGCTGGATG CCCATGTTCC AGAGGCGAAG GAGGCGAGAC 
ACCCACTTCC CCATCTGCAT TTTCTGCTGC GGCTGCTGTC ATCGATCAAA GTGTGGGATG 
TGCTGCAAGA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066998.1
CDS	51..305
Translation	MALSSQIWAACLLLLLLLASLTSGSVFPQQTGQLAELQPQDRAGARASWMPMFQRRRRRDTHFPICIFCCGCCHRSKCGMCCKT

Target ORF information:

RefSeq Version	NM_021175.4
Organism	Homo sapiens(human)
Definition	Homo sapiens hepcidin antimicrobial peptide (HAMP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021175.4

ORF Insert Sequence:

ATGGCACTGA GCTCCCAGAT CTGGGCCGCT TGCCTCCTGC TCCTCCTCCT CCTCGCCAGC 
CTGACCAGTG GCTCTGTTTT CCCACAACAG ACGGGACAAC TTGCAGAGCT GCAACCCCAG 
GACAGAGCTG GAGCCAGGGC CAGCTGGATG CCCATGTTCC AGAGGCGAAG GAGGCGAGAC 
ACCCACTTCC CCATCTGCAT TTTCTGCTGC GGCTGCTGTC ATCGATCAAA GTGTGGGATG 
TGCTGCAAGA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of novel bone morphogenetic protein- responsive elements in a hepcidin promoter.
FEBS letters591(23)3895-3905(2017 Dec)
Kanamori Y,Murakami M,Matsui T,Funaba M

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HAMP cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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