PROP1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PROP1
Entrez Gene ID	5626
Full Name	PROP paired-like homeobox 1
Synonyms	CPHD2,PROP-1
General protein information	Preferred Names PROP paired-like homeobox 1 Names homeobox protein prophet of Pit-1 pituitary-specific homeodomain factor prophet of Pit1, paired-like homeodomain transcription factor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q35.3
Summary	This gene encodes a paired-like homeodomain transcription factor in the developing pituitary gland. Expression occurs prior to and is required for expression of pou domain transcription factor 1, which is responsible for pituitary development and hormone expression. Mutations in this gene have been associated with combined pituitary hormone deficiency-2 as well as deficiencies in luteinizing hormone, follicle-stimulating hormone, growth hormone, prolactin, and thyroid-stimulating hormone. [provided by RefSeq, Sep 2011].
Disorder MIM:	601538
Disorder Html:	Pituitary hormone deficiency, combined, 2, 262600 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_006261.4	NP_006252.3	homeobox protein prophet of Pit-1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PROP1	NP_006252.3
Rattus norvegicus (Norway rat)	Prop1	NP_705891.1
Bos taurus (cattle)	PROP1	NP_777103.1
Pan troglodytes (chimpanzee)	PROP1	XP_527140.2
Mus musculus (house mouse)	Prop1	NP_032962.1
Canis lupus familiaris (dog)	PROP1	NP_001018643.1

Gene Ontology
Bibliography

Related articles in PubMed

Molecular analysis of brazilian patients with combined pituitary hormone deficiency and orthotopic posterior pituitary lobe reveals eight different PROP1 alterations with three novel mutations.
Madeira JL, Nishi MY, Nakaguma M, Benedetti AF, Biscotto IP, Fernandes T, Pequeno T, Figueiredo T, Franca MM, Correa FA, Otto AP, Abr?o M, Miras MB, Santos S, Jorge AA, Costalonga EF, Mendonca BB, Arnhold IJ, Carvalho LR
Clinical endocrinology87(6)725-732(2017 Dec)

Combined pituitary hormone deficiency due to gross deletions in the POU1F1 (PIT-1) and PROP1 genes.
Bertko E, Klammt J, Dusatkova P, Bahceci M, Gonc N, Ten Have L, Kandemir N, Mansmann G, Obermannova B, Oostdijk W, Pf?ffle H, Rockstroh-Lippold D, Schlicke M, Tuzcu AK, Pf?ffle R
Journal of human genetics62(8)755-762(2017 Aug)

Regulation of pituitary stem cells by epithelial to mesenchymal transition events and signaling pathways.
Cheung LY, Davis SW, Brinkmeier ML, Camper SA, P?rez-Mill?n MI
Molecular and cellular endocrinology44514-26(2017 Apr)

Identification of Novel PROP1 and POU1F1 Mutations in Patients with Combined Pituitary Hormone Deficiency.
Birla S, Khadgawat R, Jyotsna VP, Jain V, Garg MK, Bhalla AS, Sharma A
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme48(12)822-827(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The anterior pituitary gland comprised specialized cell-types that arise from stem cells expressing SOX2 and PROP1, which is necessary to establish the stem cell pool and promote an epithelial to mesenchymal-like transition, releasing progenitors from the niche. [review]
Title: Regulation of pituitary stem cells by epithelial to mesenchymal transition events and signaling pathways.

The present study reports on screening of POU1F1, PROP1, and HESX1 in combined pituitary hormone deficiency patients and the novel variations identified.
Title: Identification of Novel PROP1 and POU1F1 Mutations in Patients with Combined Pituitary Hormone Deficiency.

A compound heterozygosity in the PROP1 gene has been identified for both probands. The first change represents a mutational hot spot (c.150delA, p.R53fsX164), whereas the second is a novel alteration (p.R112X) that leads to protein disruption. The resulting clinical phenotype was surprisingly distinct compared to most patients with genetic alterations in PROP1.
Title: Two coexisting heterozygous frameshift mutations in PROP1 are responsible for a different phenotype of combined pituitary hormone deficiency.

Data show that human paired like homeodomain factor 1 (PROP1) can substitute functionally for mouse Prop1.
Title: All Hormone-Producing Cell Types of the Pituitary Intermediate and Anterior Lobes Derive From Prop1-Expressing Progenitors.

GH replacement successfully corrects the growth deficit in children with Growth Hormone Deficiency . While the genetic aetiology remains undefined in most cases of Isolated Growth Hormone Deficiency , PROP1 mutations constitute a major cause for Multiple Pituitary Hormone Deficiencies. Persistence of Growth Hormone Deficiency into adulthood is related to abnormal pituitary morphology.
Title: Long-Term Outcomes, Genetics, and Pituitary Morphology in Patients with Isolated Growth Hormone Deficiency and Multiple Pituitary Hormone Deficiencies: A Single-Centre Experience of Four Decades of Growth Hormone Replacement.

The following PROP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PROP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu22402	NM_006261.4 Latest version!	Homo sapiens PROP paired-like homeobox 1 (PROP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PROP1 ( NM_006261.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006252.3 Homo sapiens PROP paired-like homeobox 1 (PROP1), mRNA.

Price & Availability↑

CloneID	OHu22402
Clone ID Related Accession (Same CDS sequence)	NM_006261.4
Accession Version	NM_006261.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Homo sapiens(human)
Product	homeobox protein prophet of Pit-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF076215.1 and AC136940.2. This sequence is a reference standard in the RefSeqGene project. On Nov 4, 2009 this sequence version replaced NM_006261.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF076215.1, BC069076.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMN03465402 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGGAAGCAG AAAGGAGGCG CCAGGCTGAG AAGCCAAAGA AGGGGCGAGT CGGCAGCAGC 
CTGTTGCCTG AGAGACACCC GGCCACTGGG ACCCCGACCA CCACGGTGGA CTCGAGTGCT 
CCACCCTGCA GAAGGCTCCC TGGTGCAGGA GGGGGGAGAT CAAGGTTCTC CCCGCAAGGA 
GGACAGAGGG GCCGCCCGCA CTCCCGGCGC CGCCACCGCA CCACCTTCAG CCCAGTGCAG 
TTGGAACAGC TGGAGTCAGC CTTTGGGAGG AACCAGTACC CCGACATCTG GGCCCGAGAG 
AGTCTTGCCC GGGACACTGG CCTCAGTGAG GCCCGAATCC AGGTCTGGTT CCAGAACCGC 
AGAGCTAAGC AACGGAAGCA AGAGCGCTCA CTGCTTCAGC CTCTGGCCCA TCTGTCTCCT 
GCCGCCTTTT CCAGCTTCTT GCCAGAGTCC ACTGCTTGCC CCTATTCTTA CGCAGCACCA 
CCACCACCAG TGACCTGCTT CCCTCACCCC TACAGCCATG CCCTCCCTTC CCAGCCCTCC 
ACAGGAGGCG CCTTTGCTTT GTCACACCAG TCTGAGGACT GGTACCCTAC CTTGCACCCA 
GCCCCTGCCG GCCATCTGCC CTGCCCCCCA CCCCCTCCCA TGCTCCCCCT CAGCCTTGAG 
CCATCCAAGT CCTGGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006252.3
CDS	310..990
Translation	MEAERRRQAEKPKKGRVGSSLLPERHPATGTPTTTVDSSAPPCRRLPGAGGGRSRFSPQGGQRGRPHSRRRHRTTFSPVQLEQLESAFGRNQYPDIWARESLARDTGLSEARIQVWFQNRRAKQRKQERSLLQPLAHLSPAAFSSFLPESTACPYSYAAPPPPVTCFPHPYSHALPSQPSTGGAFALSHQSEDWYPTLHPAPAGHLPCPPPPPMLPLSLEPSKSWN

Target ORF information:

RefSeq Version	NM_006261.4
Organism	Homo sapiens(human)
Definition	Homo sapiens PROP paired-like homeobox 1 (PROP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006261.4

ORF Insert Sequence:

ATGGAAGCAG AAAGGAGGCG CCAGGCTGAG AAGCCAAAGA AGGGGCGAGT CGGCAGCAGC 
CTGTTGCCTG AGAGACACCC GGCCACTGGG ACCCCGACCA CCACGGTGGA CTCGAGTGCT 
CCACCCTGCA GAAGGCTCCC TGGTGCAGGA GGGGGGAGAT CAAGGTTCTC CCCGCAAGGA 
GGACAGAGGG GCCGCCCGCA CTCCCGGCGC CGCCACCGCA CCACCTTCAG CCCAGTGCAG 
TTGGAACAGC TGGAGTCAGC CTTTGGGAGG AACCAGTACC CCGACATCTG GGCCCGAGAG 
AGTCTTGCCC GGGACACTGG CCTCAGTGAG GCCCGAATCC AGGTCTGGTT CCAGAACCGC 
AGAGCTAAGC AACGGAAGCA AGAGCGCTCA CTGCTTCAGC CTCTGGCCCA TCTGTCTCCT 
GCCGCCTTTT CCAGCTTCTT GCCAGAGTCC ACTGCTTGCC CCTATTCTTA CGCAGCACCA 
CCACCACCAG TGACCTGCTT CCCTCACCCC TACAGCCATG CCCTCCCTTC CCAGCCCTCC 
ACAGGAGGCG CCTTTGCTTT GTCACACCAG TCTGAGGACT GGTACCCTAC CTTGCACCCA 
GCCCCTGCCG GCCATCTGCC CTGCCCCCCA CCCCCTCCCA TGCTCCCCCT CAGCCTTGAG 
CCATCCAAGT CCTGGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular analysis of brazilian patients with combined pituitary hormone deficiency and orthotopic posterior pituitary lobe reveals eight different PROP1 alterations with three novel mutations.
Clinical endocrinology87(6)725-732(2017 Dec)
Madeira JL,Nishi MY,Nakaguma M,Benedetti AF,Biscotto IP,Fernandes T,Pequeno T,Figueiredo T,Franca MM,Correa FA,Otto AP,Abr?o M,Miras MB,Santos S,Jorge AA,Costalonga EF,Mendonca BB,Arnhold IJ,Carvalho LR

Combined pituitary hormone deficiency due to gross deletions in the POU1F1 (PIT-1) and PROP1 genes.
Journal of human genetics62(8)755-762(2017 Aug)
Bertko E,Klammt J,Dusatkova P,Bahceci M,Gonc N,Ten Have L,Kandemir N,Mansmann G,Obermannova B,Oostdijk W,Pf?ffle H,Rockstroh-Lippold D,Schlicke M,Tuzcu AK,Pf?ffle R

Regulation of pituitary stem cells by epithelial to mesenchymal transition events and signaling pathways.
Molecular and cellular endocrinology44514-26(2017 Apr)
Cheung LY,Davis SW,Brinkmeier ML,Camper SA,P?rez-Mill?n MI

Identification of Novel PROP1 and POU1F1 Mutations in Patients with Combined Pituitary Hormone Deficiency.
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme48(12)822-827(2016 Dec)
Birla S,Khadgawat R,Jyotsna VP,Jain V,Garg MK,Bhalla AS,Sharma A

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

PROP1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報