NDUFA12 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NDUFA12
Entrez Gene ID	55967
Full Name	NADH:ubiquinone oxidoreductase subunit A12
Synonyms	B17.2,DAP13
General protein information	Preferred Names NADH:ubiquinone oxidoreductase subunit A12 Names NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 13 kDa differentiation-associated protein CI-B17.2 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 12 NADH-ubiquinone oxidoreductase subunit B17.2 complex I B17.2 subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q22
Summary	This gene encodes a protein which is part of mitochondrial complex 1, part of the oxidative phosphorylation system in mitochondria. Complex 1 transfers electrons to ubiquinone from NADH which establishes a proton gradient for the generation of ATP. Mutations in this gene are associated with Leigh syndrome due to mitochondrial complex 1 deficiency. Pseudogenes of this gene are located on chromosomes 5 and 13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012].
Disorder MIM:	614530
Disorder Html:	Leigh syndrome due to mitochondrial complex 1 deficiency, 256000 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_018838.5	NP_061326.1	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 isoform a
NM_018838.4	NP_061326.1	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 isoform a
NM_001258338.1	NP_001245267.1	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 isoform b

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NDUFA12	NP_001065252.1
Mus musculus (house mouse)	Ndufa12	NP_079827.2
Gallus gallus (chicken)	NDUFA12	NP_001171687.1
Bos taurus (cattle)	NDUFA12	NP_776731.1
Rattus norvegicus (Norway rat)	Ndufa12	NP_001100251.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008653	XP_314752.3
Danio rerio (zebrafish)	ndufa12	NP_001028275.1
Drosophila melanogaster (fruit fly)	CG3214	NP_001137770.1
Xenopus tropicalis (tropical clawed frog)	ndufa12	NP_001011121.1
Homo sapiens (human)	NDUFA12	NP_061326.1
	NDUFA12	XP_001106296.1
Canis lupus familiaris (dog)	NDUFA12	XP_532652.1
Caenorhabditis elegans (roundworm)	Y94H6A.8	NP_500247.2

Gene Ontology
Bibliography

Related articles in PubMed

Accessory subunits are integral for assembly and function of human mitochondrial complex I.
Stroud DA, Surgenor EE, Formosa LE, Reljic B, Frazier AE, Dibley MG, Osellame LD, Stait T, Beilharz TH, Thorburn DR, Salim A, Ryan MT
Nature538(7623)123-126(2016 Oct)

Supernumerary subunits NDUFA3, NDUFA5 and NDUFA12 are required for the formation of the extramembrane arm of human mitochondrial complex I.
Rak M, Rustin P
FEBS letters588(9)1832-8(2014 May)

Cyclin B1/Cdk1 coordinates mitochondrial respiration for cell-cycle G2/M progression.
Wang Z, Fan M, Candas D, Zhang TQ, Qin L, Eldridge A, Wachsmann-Hogiu S, Ahmed KM, Chromy BA, Nantajit D, Duru N, He F, Chen M, Finkel T, Weinstein LS, Li JJ
Developmental cell29(2)217-32(2014 Apr)

Respiratory chain complex I deficiency due to NDUFA12 mutations as a new cause of Leigh syndrome.
Ostergaard E, Rodenburg RJ, van den Brand M, Thomsen LL, Duno M, Batbayli M, Wibrand F, Nijtmans L
Journal of medical genetics48(11)737-40(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NDUFA3, NDUFA5 and NDUFA12 supernumerary subunits are necessary for complex I activity and biogenesis.
Title: Supernumerary subunits NDUFA3, NDUFA5 and NDUFA12 are required for the formation of the extramembrane arm of human mitochondrial complex I.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

The following NDUFA12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NDUFA12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10741	NM_001258338.1 Latest version!	Homo sapiens NADH:ubiquinone oxidoreductase subunit A12 (NDUFA12), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu21744	NM_018838.5 Latest version!	Homo sapiens NADH:ubiquinone oxidoreductase subunit A12 (NDUFA12), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu21744	NM_018838.4	Homo sapiens NADH:ubiquinone oxidoreductase subunit A12 (NDUFA12), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NDUFA12 ( NM_001258338.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001245267.1 Homo sapiens NADH:ubiquinone oxidoreductase subunit A12 (NDUFA12), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10741
Clone ID Related Accession (Same CDS sequence)	NM_001258338.1
Accession Version	NM_001258338.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011598.33, DW441120.1, BU532909.1 and BG943528.1. Transcript Variant: This variant (2) lacks an alternate exon which results in a frameshift and an early stop codon compared to variant 1. The resulting protein (isoform b) is shorter and has a distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BG942811.1, SRR1163658.461506.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGTTAG TGCAGGTCCT GAAACGCGGG CTGCAGCAGA TCACCGGCCA CGGCGGTCTC 
CGAGGCTATC TACGGGTTTT TTTCAGGACA AATGATGCGA AGGTTGGTAC ATTAGTGGGG 
GAAGACAAAT ATGGAAACAA ATACTATGAA GACAACAAGC AATTTTTTGG CATCGTTGGC 
TTCACAGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245267.1
CDS	34..225
Translation	MELVQVLKRGLQQITGHGGLRGYLRVFFRTNDAKVGTLVGEDKYGNKYYEDNKQFFGIVGFTV

Target ORF information:

RefSeq Version	NM_001258338.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NADH:ubiquinone oxidoreductase subunit A12 (NDUFA12), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258338.1

ORF Insert Sequence:

ATGGAGTTAG TGCAGGTCCT GAAACGCGGG CTGCAGCAGA TCACCGGCCA CGGCGGTCTC 
CGAGGCTATC TACGGGTTTT TTTCAGGACA AATGATGCGA AGGTTGGTAC ATTAGTGGGG 
GAAGACAAAT ATGGAAACAA ATACTATGAA GACAACAAGC AATTTTTTGG CATCGTTGGC 
TTCACAGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFA12 ( NM_018838.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_061326.1 Homo sapiens NADH:ubiquinone oxidoreductase subunit A12 (NDUFA12), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21744
Clone ID Related Accession (Same CDS sequence)	NM_018838.5 , NM_018838.4
Accession Version	NM_018838.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC005936.1, AC011598.33 and BG943528.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_018838.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CB109230.1, CB109322.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000327772.7/ ENSP00000330737.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGTTAG TGCAGGTCCT GAAACGCGGG CTGCAGCAGA TCACCGGCCA CGGCGGTCTC 
CGAGGCTATC TACGGGTTTT TTTCAGGACA AATGATGCGA AGGTTGGTAC ATTAGTGGGG 
GAAGACAAAT ATGGAAACAA ATACTATGAA GACAACAAGC AATTTTTTGG CCGTCACCGA 
TGGGTTGTAT ATACTACTGA AATGAATGGC AAAAACACAT TCTGGGATGT GGATGGAAGC 
ATGGTGCCTC CTGAATGGCA TCGTTGGCTT CACAGTATGA CTGATGATCC TCCAACAACA 
AAACCACTTA CTGCTCGTAA ATTCATTTGG ACGAACCATA AATTCAACGT GACTGGCACC 
CCAGAACAAT ATGTACCTTA TTCTACCACT AGAAAGAAGA TTCAGGAGTG GATCCCACCT 
TCAACACCTT ACAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061326.1
CDS	18..455
Translation	MELVQVLKRGLQQITGHGGLRGYLRVFFRTNDAKVGTLVGEDKYGNKYYEDNKQFFGRHRWVVYTTEMNGKNTFWDVDGSMVPPEWHRWLHSMTDDPPTTKPLTARKFIWTNHKFNVTGTPEQYVPYSTTRKKIQEWIPPSTPYK

Target ORF information:

RefSeq Version	NM_018838.5
Organism	Homo sapiens(human)
Definition	Homo sapiens NADH:ubiquinone oxidoreductase subunit A12 (NDUFA12), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018838.5

ORF Insert Sequence:

ATGGAGTTAG TGCAGGTCCT GAAACGCGGG CTGCAGCAGA TCACCGGCCA CGGCGGTCTC 
CGAGGCTATC TACGGGTTTT TTTCAGGACA AATGATGCGA AGGTTGGTAC ATTAGTGGGG 
GAAGACAAAT ATGGAAACAA ATACTATGAA GACAACAAGC AATTTTTTGG CCGTCACCGA 
TGGGTTGTAT ATACTACTGA AATGAATGGC AAAAACACAT TCTGGGATGT GGATGGAAGC 
ATGGTGCCTC CTGAATGGCA TCGTTGGCTT CACAGTATGA CTGATGATCC TCCAACAACA 
AAACCACTTA CTGCTCGTAA ATTCATTTGG ACGAACCATA AATTCAACGT GACTGGCACC 
CCAGAACAAT ATGTACCTTA TTCTACCACT AGAAAGAAGA TTCAGGAGTG GATCCCACCT 
TCAACACCTT ACAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFA12 ( NM_018838.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_061326.1 Homo sapiens NADH:ubiquinone oxidoreductase subunit A12 (NDUFA12), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21744
Clone ID Related Accession (Same CDS sequence)	NM_018838.5 , NM_018838.4
Accession Version	NM_018838.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011598.33, BC005936.1 and BG943528.1. This sequence is a reference standard in the RefSeqGene project. On Apr 26, 2012 this sequence version replaced NM_018838.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU943517.1, SRR1803612.102868.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGTTAG TGCAGGTCCT GAAACGCGGG CTGCAGCAGA TCACCGGCCA CGGCGGTCTC 
CGAGGCTATC TACGGGTTTT TTTCAGGACA AATGATGCGA AGGTTGGTAC ATTAGTGGGG 
GAAGACAAAT ATGGAAACAA ATACTATGAA GACAACAAGC AATTTTTTGG CCGTCACCGA 
TGGGTTGTAT ATACTACTGA AATGAATGGC AAAAACACAT TCTGGGATGT GGATGGAAGC 
ATGGTGCCTC CTGAATGGCA TCGTTGGCTT CACAGTATGA CTGATGATCC TCCAACAACA 
AAACCACTTA CTGCTCGTAA ATTCATTTGG ACGAACCATA AATTCAACGT GACTGGCACC 
CCAGAACAAT ATGTACCTTA TTCTACCACT AGAAAGAAGA TTCAGGAGTG GATCCCACCT 
TCAACACCTT ACAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061326.1
CDS	34..471
Translation	MELVQVLKRGLQQITGHGGLRGYLRVFFRTNDAKVGTLVGEDKYGNKYYEDNKQFFGRHRWVVYTTEMNGKNTFWDVDGSMVPPEWHRWLHSMTDDPPTTKPLTARKFIWTNHKFNVTGTPEQYVPYSTTRKKIQEWIPPSTPYK

Target ORF information:

RefSeq Version	NM_018838.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NADH:ubiquinone oxidoreductase subunit A12 (NDUFA12), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018838.4

ORF Insert Sequence:

ATGGAGTTAG TGCAGGTCCT GAAACGCGGG CTGCAGCAGA TCACCGGCCA CGGCGGTCTC 
CGAGGCTATC TACGGGTTTT TTTCAGGACA AATGATGCGA AGGTTGGTAC ATTAGTGGGG 
GAAGACAAAT ATGGAAACAA ATACTATGAA GACAACAAGC AATTTTTTGG CCGTCACCGA 
TGGGTTGTAT ATACTACTGA AATGAATGGC AAAAACACAT TCTGGGATGT GGATGGAAGC 
ATGGTGCCTC CTGAATGGCA TCGTTGGCTT CACAGTATGA CTGATGATCC TCCAACAACA 
AAACCACTTA CTGCTCGTAA ATTCATTTGG ACGAACCATA AATTCAACGT GACTGGCACC 
CCAGAACAAT ATGTACCTTA TTCTACCACT AGAAAGAAGA TTCAGGAGTG GATCCCACCT 
TCAACACCTT ACAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Accessory subunits are integral for assembly and function of human mitochondrial complex I.
Nature538(7623)123-126(2016 Oct)
Stroud DA,Surgenor EE,Formosa LE,Reljic B,Frazier AE,Dibley MG,Osellame LD,Stait T,Beilharz TH,Thorburn DR,Salim A,Ryan MT

Supernumerary subunits NDUFA3, NDUFA5 and NDUFA12 are required for the formation of the extramembrane arm of human mitochondrial complex I.
FEBS letters588(9)1832-8(2014 May)
Rak M,Rustin P

Cyclin B1/Cdk1 coordinates mitochondrial respiration for cell-cycle G2/M progression.
Developmental cell29(2)217-32(2014 Apr)
Wang Z,Fan M,Candas D,Zhang TQ,Qin L,Eldridge A,Wachsmann-Hogiu S,Ahmed KM,Chromy BA,Nantajit D,Duru N,He F,Chen M,Finkel T,Weinstein LS,Li JJ

Respiratory chain complex I deficiency due to NDUFA12 mutations as a new cause of Leigh syndrome.
Journal of medical genetics48(11)737-40(2011 Nov)
Ostergaard E,Rodenburg RJ,van den Brand M,Thomsen LL,Duno M,Batbayli M,Wibrand F,Nijtmans L

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NDUFA12 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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