PPP4R3A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPP4R3A
Entrez Gene ID	55671
Full Name	protein phosphatase 4 regulatory subunit 3A
Synonyms	FLFL1,KIAA2010,MSTP033,PP4R3,PP4R3A,SMEK1,smk-1,smk1
General protein information	Preferred Names protein phosphatase 4 regulatory subunit 3A Names serine/threonine-protein phosphatase 4 regulatory subunit 3A SMEK homolog 1, suppressor of mek1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q32.12
Disorder MIM:	610351

mRNA and Protein(s)

mRNA	Protein	Name
NM_001284281.1	NP_001271210.1	serine/threonine-protein phosphatase 4 regulatory subunit 3A isoform 3
NM_001284280.1	NP_001271209.1	serine/threonine-protein phosphatase 4 regulatory subunit 3A isoform 1
NM_001366432.1	NP_001353361.1	serine/threonine-protein phosphatase 4 regulatory subunit 3A isoform 4
XM_005267842.2	XP_005267899.1	serine/threonine-protein phosphatase 4 regulatory subunit 3A isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SMEK1	NP_001271209.1
Pan troglodytes (chimpanzee)	SMEK1	XP_001140788.2
Bos taurus (cattle)	SMEK1	NP_001192449.1
Arabidopsis thaliana (thale cress)	AT3G06670	NP_001189831.1
Canis lupus familiaris (dog)	SMEK1	XP_005623804.1
Magnaporthe oryzae (rice blast fungus)	MGG_06138	XP_003712012.1
Xenopus tropicalis (tropical clawed frog)	LOC100485426	XP_002933248.1
Mus musculus (house mouse)	Smek1	NP_001153686.1
Danio rerio (zebrafish)	smek1	NP_001038274.1
Caenorhabditis elegans (roundworm)	smk-1	NP_001023909.2
Schizosaccharomyces pombe (fission yeast)	SPBC216.01c	XP_001713123.1
Rattus norvegicus (Norway rat)	Smek1	NP_001258103.1
Gallus gallus (chicken)	SMEK1	XP_421321.2
Neurospora crassa	NCU11315	XP_001728203.1
Oryza sativa (rice)	Os01g0337600	NP_001042939.2

Gene Ontology
Bibliography

Related articles in PubMed

A variant in PPP4R3A protects against alzheimer-related metabolic decline.
Christopher L, Napolioni V, Khan RR, Han SS, Greicius MD,
Annals of neurology82(6)900-911(2017 Dec)

BMI-1 interacts with sMEK1 and inactivates sMEK1-induced apoptotic cell death.
Kim BR, Kwon Y, Rho SB
Oncology reports37(1)579-586(2017 Jan)

sMEK1 inhibits endothelial cell proliferation by attenuating VEGFR-2-dependent-Akt/eNOS/HIF-1? signaling pathways.
Kim BR, Seo SH, Park MS, Lee SH, Kwon Y, Rho SB
Oncotarget6(31)31830-43(2015 Oct)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

Tumor suppressor BLU enhances pro-apoptotic activity of sMEK1 through physical interaction.
Dong SM, Byun HJ, Kim BR, Lee SH, Trink B, Rho SB
Cellular signalling24(6)1208-14(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A variant in PPP4R3A was associated with reduced metabolic decline in Alzheimer's disease patients.
Title: A variant in PPP4R3A protects against alzheimer-related metabolic decline.

sMEK1 significantly inhibited BMI-1-stimulated oncogenesis.
Title: BMI-1 interacts with sMEK1 and inactivates sMEK1-induced apoptotic cell death.

Loss of sMEK1 is associated with Ovarian Neoplasms.
Title: sMEK1 inhibits endothelial cell proliferation by attenuating VEGFR-2-dependent-Akt/eNOS/HIF-1? signaling pathways.

The N-terminal of BLU was observed to interact with the C-terminal of SMEK1, a regulatory subunit of protein phosphatase 4. Furthermore, we determined the binding domains that are required for interaction between BLU and sMEK1. The N-terminal of BLU was observed to interact with the C-terminal of sMEK1.
Title: Tumor suppressor BLU enhances pro-apoptotic activity of sMEK1 through physical interaction.

The following PPP4R3A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPP4R3A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu00426	NM_001284281.1 Latest version!	Homo sapiens protein phosphatase 4 regulatory subunit 3A (PPP4R3A), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu05311	NM_001284280.1 Latest version!	Homo sapiens protein phosphatase 4 regulatory subunit 3A (PPP4R3A), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu47788	NM_001366432.1 Latest version!	Homo sapiens protein phosphatase 4 regulatory subunit 3A (PPP4R3A), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu47788	XM_005267842.2 Latest version!	Homo sapiens protein phosphatase 4 regulatory subunit 3A (PPP4R3A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPP4R3A ( NM_001284281.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271210.1 Homo sapiens protein phosphatase 4 regulatory subunit 3A (PPP4R3A), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu00426
Clone ID Related Accession (Same CDS sequence)	NM_001284281.1
Accession Version	NM_001284281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-15
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 4 regulatory subunit 3A isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC022778.1, BC038932.1, BC028088.1 and AI608781.1. On Sep 27, 2013 this sequence version replaced XM_005267843.1. Transcript Variant: This variant (4) lacks two alternate in-frame exons and uses an alternate in-frame splice site, compared to variant 3. The encoded isoform (3) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC038932.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2467147 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCGACA CCCGGCGGCG GGTGAAGGTG TACACGCTCA ACGAGGACCG GCAGTGGGAC 
GACCGGGGCA CCGGGCATGT GTCGTCTGGC TACGTGGAGC GGCTGAAGGG CATGTCCCTG 
CTTGTCAGGG CTGAGAGCGA CGGTTCTCTA CTTTTAGAGT CGAAAATAAA TCCTAACACT 
GCATACCAGA AACAACAGGA AGATGAAAAA TTTCTGACAG ATTTGTTTGC ACAACTAACA 
GATGAAGCAA CAGATGAGGA AAAAAGACAG GAATTGGTTA ACTTTTTAAA AGAATTTTGT 
GCGTTTTCCC AAACGCTACA GCCTCAAAAC AGAGATGCTT TTTTCAAGAC TTTGTCAAAC 
ATGGGCATAT TACCAGCTTT AGAAGTCATC CTTGGCATGG ATGATACACA GGTGCGAAGT 
GCTGCTACTG ATATATTCTC ATACTTGGTT GAATATAATC CATCCATGGT ACGAGAGTTT 
GTCATGCAGG AGGCACAACA GAATGATGAT GTAAGTAAGA AGTTAACAGA GCAAAAAATA 
ACCAGCAAGG ATATTTTGCT CATCAACCTC ATTATAGAAC ATATGATTTG TGATACAGAT 
CCTGAACTTG GAGGAGCAGT CCAGCTTATG GGCCTGCTTC GAACTTTAGT TGACCCAGAG 
AACATGCTAG CCACTGCCAA TAAAACAGAA AAGACTGAAT TTCTGGGTTT CTTCTACAAG 
CACTGTATGC ATGTTCTCAC TGCTCCTTTA CTAGCAAATA CAACAGAAGA CAAACCTAGT 
AAAGATGATT TTCAGACTGC CCAACTATTG GCACTTGTAT TGGAATTGTT AACATTTTGT 
GTGGAGCACC ATACCTACCA CATAAAGAAC TACATTATTA ATAAGGATAT CCTCCGGAGA 
GTGCTAGTTC TTATGGCCTC GAAGCATGCT TTCTTGGCAT TATGTGCCCT TCGTTTTAAA 
AGAAAGATTA TTGGATTAAA AGATGAGTTT TACAACCGCT ACATAATGAA AAGTTTTTTG 
TTTGAACCAG TAGTGAAAGC ATTTCTCAAC AATGGATCCC GCTACAATCT GATGAACTCT 
GCCATAATAG AGATGTTTGA ATTTATTAGA GTGGAAGATA TAAAATCATT AACTGCTCAT 
GTAATTGAAA ATTACTGGAA AGCACTGGAA GATGTAGATT ATGTACAGAC ATTTAAAGGA 
TTAAAACTGA GATTTGAACA ACAAAGAGAA AGGCAAGATA ATCCCAAACT TGACAGTATG 
CGTTCCATTT TGAGGAATCA CAGATATCGA AGAGATGCCA GAACACTAGA AGATGAAGAA 
GAGATGTGGT TTAACACAGA TGAAGATGAC ATGGAAGATG GAGAAGCTGT AGTGTCTCCA 
TCTGACAAAA CTAAAAATGA TGATGATATT ATGGATCCAA TAAGTAAATT CATGGAAAGG 
AAGAAATTAA AAGAAAGTGA GGAAAAGGAA GTGCTTCTGA AAACAAACCT TTCTGGACGG 
CAGAGCCCAA GTTTCAAGCT TTCCCTGTCC AGTGGAACGA AGACTAACCT CACCAGCCAG 
TCATCTACAA CAAATCTGCC TGGTTCTCCG GGATCACCTG GATCCCCAGG ATCTCCAGGC 
TCTCCTGGAT CCGTACCTAA AAATACATCT CAGACGGCAG CTATTACTAC AAAGGGAGGC 
CTCGTGGGTC TGGTAGATTA TCCTGATGAT GATGAAGATG ATGATGAGGA TGAAGATAAG 
GAAGATACGT TACCATTGTC AAAGAAAGCA AAATTTGATT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271210.1
CDS	834..2618
Translation	MTDTRRRVKVYTLNEDRQWDDRGTGHVSSGYVERLKGMSLLVRAESDGSLLLESKINPNTAYQKQQEDEKFLTDLFAQLTDEATDEEKRQELVNFLKEFCAFSQTLQPQNRDAFFKTLSNMGILPALEVILGMDDTQVRSAATDIFSYLVEYNPSMVREFVMQEAQQNDDVSKKLTEQKITSKDILLINLIIEHMICDTDPELGGAVQLMGLLRTLVDPENMLATANKTEKTEFLGFFYKHCMHVLTAPLLANTTEDKPSKDDFQTAQLLALVLELLTFCVEHHTYHIKNYIINKDILRRVLVLMASKHAFLALCALRFKRKIIGLKDEFYNRYIMKSFLFEPVVKAFLNNGSRYNLMNSAIIEMFEFIRVEDIKSLTAHVIENYWKALEDVDYVQTFKGLKLRFEQQRERQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDDMEDGEAVVSPSDKTKNDDDIMDPISKFMERKKLKESEEKEVLLKTNLSGRQSPSFKLSLSSGTKTNLTSQSSTTNLPGSPGSPGSPGSPGSPGSVPKNTSQTAAITTKGGLVGLVDYPDDDEDDDEDEDKEDTLPLSKKAKFDS

Target ORF information:

RefSeq Version	NM_001284281.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 4 regulatory subunit 3A (PPP4R3A), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284281.1

ORF Insert Sequence:

ATGACCGACA CCCGGCGGCG GGTGAAGGTG TACACGCTCA ACGAGGACCG GCAGTGGGAC 
GACCGGGGCA CCGGGCATGT GTCGTCTGGC TACGTGGAGC GGCTGAAGGG CATGTCCCTG 
CTTGTCAGGG CTGAGAGCGA CGGTTCTCTA CTTTTAGAGT CGAAAATAAA TCCTAACACT 
GCATACCAGA AACAACAGGA AGATGAAAAA TTTCTGACAG ATTTGTTTGC ACAACTAACA 
GATGAAGCAA CAGATGAGGA AAAAAGACAG GAATTGGTTA ACTTTTTAAA AGAATTTTGT 
GCGTTTTCCC AAACGCTACA GCCTCAAAAC AGAGATGCTT TTTTCAAGAC TTTGTCAAAC 
ATGGGCATAT TACCAGCTTT AGAAGTCATC CTTGGCATGG ATGATACACA GGTGCGAAGT 
GCTGCTACTG ATATATTCTC ATACTTGGTT GAATATAATC CATCCATGGT ACGAGAGTTT 
GTCATGCAGG AGGCACAACA GAATGATGAT GTAAGTAAGA AGTTAACAGA GCAAAAAATA 
ACCAGCAAGG ATATTTTGCT CATCAACCTC ATTATAGAAC ATATGATTTG TGATACAGAT 
CCTGAACTTG GAGGAGCAGT CCAGCTTATG GGCCTGCTTC GAACTTTAGT TGACCCAGAG 
AACATGCTAG CCACTGCCAA TAAAACAGAA AAGACTGAAT TTCTGGGTTT CTTCTACAAG 
CACTGTATGC ATGTTCTCAC TGCTCCTTTA CTAGCAAATA CAACAGAAGA CAAACCTAGT 
AAAGATGATT TTCAGACTGC CCAACTATTG GCACTTGTAT TGGAATTGTT AACATTTTGT 
GTGGAGCACC ATACCTACCA CATAAAGAAC TACATTATTA ATAAGGATAT CCTCCGGAGA 
GTGCTAGTTC TTATGGCCTC GAAGCATGCT TTCTTGGCAT TATGTGCCCT TCGTTTTAAA 
AGAAAGATTA TTGGATTAAA AGATGAGTTT TACAACCGCT ACATAATGAA AAGTTTTTTG 
TTTGAACCAG TAGTGAAAGC ATTTCTCAAC AATGGATCCC GCTACAATCT GATGAACTCT 
GCCATAATAG AGATGTTTGA ATTTATTAGA GTGGAAGATA TAAAATCATT AACTGCTCAT 
GTAATTGAAA ATTACTGGAA AGCACTGGAA GATGTAGATT ATGTACAGAC ATTTAAAGGA 
TTAAAACTGA GATTTGAACA ACAAAGAGAA AGGCAAGATA ATCCCAAACT TGACAGTATG 
CGTTCCATTT TGAGGAATCA CAGATATCGA AGAGATGCCA GAACACTAGA AGATGAAGAA 
GAGATGTGGT TTAACACAGA TGAAGATGAC ATGGAAGATG GAGAAGCTGT AGTGTCTCCA 
TCTGACAAAA CTAAAAATGA TGATGATATT ATGGATCCAA TAAGTAAATT CATGGAAAGG 
AAGAAATTAA AAGAAAGTGA GGAAAAGGAA GTGCTTCTGA AAACAAACCT TTCTGGACGG 
CAGAGCCCAA GTTTCAAGCT TTCCCTGTCC AGTGGAACGA AGACTAACCT CACCAGCCAG 
TCATCTACAA CAAATCTGCC TGGTTCTCCG GGATCACCTG GATCCCCAGG ATCTCCAGGC 
TCTCCTGGAT CCGTACCTAA AAATACATCT CAGACGGCAG CTATTACTAC AAAGGGAGGC 
CTCGTGGGTC TGGTAGATTA TCCTGATGAT GATGAAGATG ATGATGAGGA TGAAGATAAG 
GAAGATACGT TACCATTGTC AAAGAAAGCA AAATTTGATT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP4R3A ( NM_001284280.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271209.1 Homo sapiens protein phosphatase 4 regulatory subunit 3A (PPP4R3A), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu05311
Clone ID Related Accession (Same CDS sequence)	NM_001284280.1
Accession Version	NM_001284280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 4 regulatory subunit 3A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC022778.1, AB095930.1, BC028088.1 and AI608781.1. Transcript Variant: This variant (3) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AB095930.1, SRR1660809.70296.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467147 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCGACA CCCGGCGGCG GGTGAAGGTG TACACGCTCA ACGAGGACCG GCAGTGGGAC 
GACCGGGGCA CCGGGCATGT GTCGTCTGGC TACGTGGAGC GGCTGAAGGG CATGTCCCTG 
CTTGTCAGGG CTGAGAGCGA CGGTTCTCTA CTTTTAGAGT CGAAAATAAA TCCTAACACT 
GCATACCAGA AACAACAGGA CACTCTGATT GTGTGGTCTG AAGCAGAAAA TTATGACTTG 
GCCCTTAGCT TTCAAGAAAA AGCTGGATGT GATGAAATTT GGGAGAAAAT ATGTCAGGTT 
CAAGGAAAGG ACCCTTCCGT GGACATCACT CAGGACCTTG TGGATGAATC TGAAGAGGAG 
CGTTTTGATG ATATGTCATC GCCAGGCTTA GAATTGCCAT CTTGTGAATT AAGTCGCCTT 
GAAGAAATTG CAGAACTTGT GGCATCATCT TTACCTTCAC CTCTTCGTCG TGAAAAACTT 
GCACTGGCAC TAGAAAATGA GGGTTATATT AAAAAGCTCC TGGAGCTTTT TCATGTGTGT 
GAAGATTTGG AAAATATTGA AGGACTGCAC CACTTGTATG AAATTATCAA AGGCATCTTT 
CTCTTGAATC GAACTGCTCT TTTTGAAGTT ATGTTCTCTG AAGAATGTAT AATGGACGTC 
ATTGGATGTT TAGAATATGA TCCTGCTTTA TCACAACCAC GAAAACACAG GGAATTTCTA 
ACAAAAACAG CCAAGTTTAA AGAAGTGATT CCCATATCAG ATCCTGAGCT GAAACAAAAA 
ATTCATCAGA CATACAGAGT TCAGTATATA CAAGATATGG TTCTACCAAC TCCTTCGGTC 
TTTGAAGAAA ACATGTTATC AACACTTCAC TCTTTTATCT TTTTCAATAA GGTAGAGATT 
GTTGGCATGT TGCAGGAAGA TGAAAAATTT CTGACAGATT TGTTTGCACA ACTAACAGAT 
GAAGCAACAG ATGAGGAAAA AAGACAGGAA TTGGTTAACT TTTTAAAAGA ATTTTGTGCG 
TTTTCCCAAA CGCTACAGCC TCAAAACAGA GATGCTTTTT TCAAGACTTT GTCAAACATG 
GGCATATTAC CAGCTTTAGA AGTCATCCTT GGCATGGATG ATACACAGGT GCGAAGTGCT 
GCTACTGATA TATTCTCATA CTTGGTTGAA TATAATCCAT CCATGGTACG AGAGTTTGTC 
ATGCAGGAGG CACAACAGAA TGATGATGAT ATTTTGCTCA TCAACCTCAT TATAGAACAT 
ATGATTTGTG ATACAGATCC TGAACTTGGA GGAGCAGTCC AGCTTATGGG CCTGCTTCGA 
ACTTTAGTTG ACCCAGAGAA CATGCTAGCC ACTGCCAATA AAACAGAAAA GACTGAATTT 
CTGGGTTTCT TCTACAAGCA CTGTATGCAT GTTCTCACTG CTCCTTTACT AGCAAATACA 
ACAGAAGACA AACCTAGTAA AGATGATTTT CAGACTGCCC AACTATTGGC ACTTGTATTG 
GAATTGTTAA CATTTTGTGT GGAGCACCAT ACCTACCACA TAAAGAACTA CATTATTAAT 
AAGGATATCC TCCGGAGAGT GCTAGTTCTT ATGGCCTCGA AGCATGCTTT CTTGGCATTA 
TGTGCCCTTC GTTTTAAAAG AAAGATTATT GGATTAAAAG ATGAGTTTTA CAACCGCTAC 
ATAATGAAAA GTTTTTTGTT TGAACCAGTA GTGAAAGCAT TTCTCAACAA TGGATCCCGC 
TACAATCTGA TGAACTCTGC CATAATAGAG ATGTTTGAAT TTATTAGAGT GGAAGATATA 
AAATCATTAA CTGCTCATGT AATTGAAAAT TACTGGAAAG CACTGGAAGA TGTAGATTAT 
GTACAGACAT TTAAAGGATT AAAACTGAGA TTTGAACAAC AAAGAGAAAG GCAAGATAAT 
CCCAAACTTG ACAGTATGCG TTCCATTTTG AGGAATCACA GATATCGAAG AGATGCCAGA 
ACACTAGAAG ATGAAGAAGA GATGTGGTTT AACACAGATG AAGATGACAT GGAAGATGGA 
GAAGCTGTAG TGTCTCCATC TGACAAAACT AAAAATGATG ATGATATTAT GGATCCAATA 
AGTAAATTCA TGGAAAGGAA GAAATTAAAA GAAAGTGAGG AAAAGGAAGT GCTTCTGAAA 
ACAAACCTTT CTGGACGGCA GAGCCCAAGT TTCAAGCTTT CCCTGTCCAG TGGAACGAAG 
ACTAACCTCA CCAGCCAGTC ATCTACAACA AATCTGCCTG GTTCTCCGGG ATCACCTGGA 
TCCCCAGGAT CTCCAGGCTC TCCTGGATCC GTACCTAAAA ATACATCTCA GACGGCAGCT 
ATTACTACAA AGGGAGGCCT CGTGGGTCTG GTAGATTATC CTGATGATGA TGAAGATGAT 
GATGAGGATG AAGATAAGGA AGATACGTTA CCATTGTCAA AGAAAGCAAA ATTTGATTCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271209.1
CDS	834..3296
Translation	MTDTRRRVKVYTLNEDRQWDDRGTGHVSSGYVERLKGMSLLVRAESDGSLLLESKINPNTAYQKQQDTLIVWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVDITQDLVDESEEERFDDMSSPGLELPSCELSRLEEIAELVASSLPSPLRREKLALALENEGYIKKLLELFHVCEDLENIEGLHHLYEIIKGIFLLNRTALFEVMFSEECIMDVIGCLEYDPALSQPRKHREFLTKTAKFKEVIPISDPELKQKIHQTYRVQYIQDMVLPTPSVFEENMLSTLHSFIFFNKVEIVGMLQEDEKFLTDLFAQLTDEATDEEKRQELVNFLKEFCAFSQTLQPQNRDAFFKTLSNMGILPALEVILGMDDTQVRSAATDIFSYLVEYNPSMVREFVMQEAQQNDDDILLINLIIEHMICDTDPELGGAVQLMGLLRTLVDPENMLATANKTEKTEFLGFFYKHCMHVLTAPLLANTTEDKPSKDDFQTAQLLALVLELLTFCVEHHTYHIKNYIINKDILRRVLVLMASKHAFLALCALRFKRKIIGLKDEFYNRYIMKSFLFEPVVKAFLNNGSRYNLMNSAIIEMFEFIRVEDIKSLTAHVIENYWKALEDVDYVQTFKGLKLRFEQQRERQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDDMEDGEAVVSPSDKTKNDDDIMDPISKFMERKKLKESEEKEVLLKTNLSGRQSPSFKLSLSSGTKTNLTSQSSTTNLPGSPGSPGSPGSPGSPGSVPKNTSQTAAITTKGGLVGLVDYPDDDEDDDEDEDKEDTLPLSKKAKFDS

Target ORF information:

RefSeq Version	NM_001284280.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 4 regulatory subunit 3A (PPP4R3A), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284280.1

ORF Insert Sequence:

ATGACCGACA CCCGGCGGCG GGTGAAGGTG TACACGCTCA ACGAGGACCG GCAGTGGGAC 
GACCGGGGCA CCGGGCATGT GTCGTCTGGC TACGTGGAGC GGCTGAAGGG CATGTCCCTG 
CTTGTCAGGG CTGAGAGCGA CGGTTCTCTA CTTTTAGAGT CGAAAATAAA TCCTAACACT 
GCATACCAGA AACAACAGGA CACTCTGATT GTGTGGTCTG AAGCAGAAAA TTATGACTTG 
GCCCTTAGCT TTCAAGAAAA AGCTGGATGT GATGAAATTT GGGAGAAAAT ATGTCAGGTT 
CAAGGAAAGG ACCCTTCCGT GGACATCACT CAGGACCTTG TGGATGAATC TGAAGAGGAG 
CGTTTTGATG ATATGTCATC GCCAGGCTTA GAATTGCCAT CTTGTGAATT AAGTCGCCTT 
GAAGAAATTG CAGAACTTGT GGCATCATCT TTACCTTCAC CTCTTCGTCG TGAAAAACTT 
GCACTGGCAC TAGAAAATGA GGGTTATATT AAAAAGCTCC TGGAGCTTTT TCATGTGTGT 
GAAGATTTGG AAAATATTGA AGGACTGCAC CACTTGTATG AAATTATCAA AGGCATCTTT 
CTCTTGAATC GAACTGCTCT TTTTGAAGTT ATGTTCTCTG AAGAATGTAT AATGGACGTC 
ATTGGATGTT TAGAATATGA TCCTGCTTTA TCACAACCAC GAAAACACAG GGAATTTCTA 
ACAAAAACAG CCAAGTTTAA AGAAGTGATT CCCATATCAG ATCCTGAGCT GAAACAAAAA 
ATTCATCAGA CATACAGAGT TCAGTATATA CAAGATATGG TTCTACCAAC TCCTTCGGTC 
TTTGAAGAAA ACATGTTATC AACACTTCAC TCTTTTATCT TTTTCAATAA GGTAGAGATT 
GTTGGCATGT TGCAGGAAGA TGAAAAATTT CTGACAGATT TGTTTGCACA ACTAACAGAT 
GAAGCAACAG ATGAGGAAAA AAGACAGGAA TTGGTTAACT TTTTAAAAGA ATTTTGTGCG 
TTTTCCCAAA CGCTACAGCC TCAAAACAGA GATGCTTTTT TCAAGACTTT GTCAAACATG 
GGCATATTAC CAGCTTTAGA AGTCATCCTT GGCATGGATG ATACACAGGT GCGAAGTGCT 
GCTACTGATA TATTCTCATA CTTGGTTGAA TATAATCCAT CCATGGTACG AGAGTTTGTC 
ATGCAGGAGG CACAACAGAA TGATGATGAT ATTTTGCTCA TCAACCTCAT TATAGAACAT 
ATGATTTGTG ATACAGATCC TGAACTTGGA GGAGCAGTCC AGCTTATGGG CCTGCTTCGA 
ACTTTAGTTG ACCCAGAGAA CATGCTAGCC ACTGCCAATA AAACAGAAAA GACTGAATTT 
CTGGGTTTCT TCTACAAGCA CTGTATGCAT GTTCTCACTG CTCCTTTACT AGCAAATACA 
ACAGAAGACA AACCTAGTAA AGATGATTTT CAGACTGCCC AACTATTGGC ACTTGTATTG 
GAATTGTTAA CATTTTGTGT GGAGCACCAT ACCTACCACA TAAAGAACTA CATTATTAAT 
AAGGATATCC TCCGGAGAGT GCTAGTTCTT ATGGCCTCGA AGCATGCTTT CTTGGCATTA 
TGTGCCCTTC GTTTTAAAAG AAAGATTATT GGATTAAAAG ATGAGTTTTA CAACCGCTAC 
ATAATGAAAA GTTTTTTGTT TGAACCAGTA GTGAAAGCAT TTCTCAACAA TGGATCCCGC 
TACAATCTGA TGAACTCTGC CATAATAGAG ATGTTTGAAT TTATTAGAGT GGAAGATATA 
AAATCATTAA CTGCTCATGT AATTGAAAAT TACTGGAAAG CACTGGAAGA TGTAGATTAT 
GTACAGACAT TTAAAGGATT AAAACTGAGA TTTGAACAAC AAAGAGAAAG GCAAGATAAT 
CCCAAACTTG ACAGTATGCG TTCCATTTTG AGGAATCACA GATATCGAAG AGATGCCAGA 
ACACTAGAAG ATGAAGAAGA GATGTGGTTT AACACAGATG AAGATGACAT GGAAGATGGA 
GAAGCTGTAG TGTCTCCATC TGACAAAACT AAAAATGATG ATGATATTAT GGATCCAATA 
AGTAAATTCA TGGAAAGGAA GAAATTAAAA GAAAGTGAGG AAAAGGAAGT GCTTCTGAAA 
ACAAACCTTT CTGGACGGCA GAGCCCAAGT TTCAAGCTTT CCCTGTCCAG TGGAACGAAG 
ACTAACCTCA CCAGCCAGTC ATCTACAACA AATCTGCCTG GTTCTCCGGG ATCACCTGGA 
TCCCCAGGAT CTCCAGGCTC TCCTGGATCC GTACCTAAAA ATACATCTCA GACGGCAGCT 
ATTACTACAA AGGGAGGCCT CGTGGGTCTG GTAGATTATC CTGATGATGA TGAAGATGAT 
GATGAGGATG AAGATAAGGA AGATACGTTA CCATTGTCAA AGAAAGCAAA ATTTGATTCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP4R3A ( NM_001366432.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001353361.1 Homo sapiens protein phosphatase 4 regulatory subunit 3A (PPP4R3A), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu47788
Clone ID Related Accession (Same CDS sequence)	NM_001366432.1 , XM_005267842.2
Accession Version	NM_001366432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 4 regulatory subunit 3A isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL133153.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.158082.1, SRR1803613.268076.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467147 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGACCGACA CCCGGCGGCG GGTGAAGGTG TACACGCTCA ACGAGGACCG GCAGTGGGAC 
GACCGGGGCA CCGGGCATGT GTCGTCTGGC TACGTGGAGC GGCTGAAGGG CATGTCCCTG 
CTTGTCAGGG CTGAGAGCGA CGGTTCTCTA CTTTTAGAGT CGAAAATAAA TCCTAACACT 
GCATACCAGA AACAACAGGA CACTCTGATT GTGTGGTCTG AAGCAGAAAA TTATGACTTG 
GCCCTTAGCT TTCAAGAAAA AGCTGGATGT GATGAAATTT GGGAGAAAAT ATGTCAGGTT 
CAAGGAAAGG ACCCTTCCGT GGACATCACT CAGGACCTTG TGGATGAATC TGAAGAGGAG 
CGTTTTGATG ATATGTCATC GCCAGGCTTA GAATTGCCAT CTTGTGAATT AAGTCGCCTT 
GAAGAAATTG CAGAACTTGT GGCATCATCT TTACCTTCAC CTCTTCGTCG TGAAAAACTT 
GCACTGGCAC TAGAAAATGA GGGTTATATT AAAAAGCTCC TGGAGCTTTT TCATGTGTGT 
GAAGATTTGG AAAATATTGA AGGACTGCAC CACTTGTATG AAATTATCAA AGGCATCTTT 
CTCTTGAATC GAACTGCTCT TTTTGAAGTT ATGTTCTCTG AAGAATGTAT AATGGACGTC 
ATTGGATGTT TAGAATATGA TCCTGCTTTA TCACAACCAC GAAAACACAG GGAATTTCTA 
ACAAAAACAG CCAAGTTTAA AGAAGTGATT CCCATATCAG ATCCTGAGCT GAAACAAAAA 
ATTCATCAGA CATACAGAGT TCAGTATATA CAAGATATGG TTCTACCAAC TCCTTCGGTC 
TTTGAAGAAA ACATGTTATC AACACTTCAC TCTTTTATCT TTTTCAATAA GGTAGAGATT 
GTTGGCATGT TGCAGGAAGA TGAAAAATTT CTGACAGATT TGTTTGCACA ACTAACAGAT 
GAAGCAACAG ATGAGGAAAA AAGACAGGAA TTGGTTAACT TTTTAAAAGA ATTTTGTGCG 
TTTTCCCAAA CGCTACAGCC TCAAAACAGA GATGCTTTTT TCAAGACTTT GTCAAACATG 
GGCATATTAC CAGCTTTAGA AGTCATCCTT GGCATGGATG ATACACAGGT GCGAAGTGCT 
GCTACTGATA TATTCTCATA CTTGGTTGAA TATAATCCAT CCATGGTACG AGAGTTTGTC 
ATGCAGGAGG CACAACAGAA TGATGATGTA AGTAAGAAGT TAACAGAGCA AAAAATAACC 
AGCAAGGATA TTTTGCTCAT CAACCTCATT ATAGAACATA TGATTTGTGA TACAGATCCT 
GAACTTGGAG GAGCAGTCCA GCTTATGGGC CTGCTTCGAA CTTTAGTTGA CCCAGAGAAC 
ATGCTAGCCA CTGCCAATAA AACAGAAAAG ACTGAATTTC TGGGTTTCTT CTACAAGCAC 
TGTATGCATG TTCTCACTGC TCCTTTACTA GCAAATACAA CAGAAGACAA ACCTAGTAAA 
GATGATTTTC AGACTGCCCA ACTATTGGCA CTTGTATTGG AATTGTTAAC ATTTTGTGTG 
GAGCACCATA CCTACCACAT AAAGAACTAC ATTATTAATA AGGATATCCT CCGGAGAGTG 
CTAGTTCTTA TGGCCTCGAA GCATGCTTTC TTGGCATTAT GTGCCCTTCG TTTTAAAAGA 
AAGATTATTG GATTAAAAGA TGAGTTTTAC AACCGCTACA TAATGAAAAG TTTTTTGTTT 
GAACCAGTAG TGAAAGCATT TCTCAACAAT GGATCCCGCT ACAATCTGAT GAACTCTGCC 
ATAATAGAGA TGTTTGAATT TATTAGAGTG GAAGATATAA AATCATTAAC TGCTCATGTA 
ATTGAAAATT ACTGGAAAGC ACTGGAAGAT GTAGATTATG TACAGACATT TAAAGGATTA 
AAACTGAGAT TTGAACAACA AAGAGAAAGG CAAGATAATC CCAAACTTGA CAGTATGCGT 
TCCATTTTGA GGAATCACAG ATATCGAAGA GATGCCAGAA CACTAGAAGA TGAAGAAGAG 
ATGTGGTTTA ACACAGATGA AGATGACATG GAAGATGGAG AAGCTGTAGT GTCTCCATCT 
GACAAAACTA AAAATGATGA TGATATTATG GATCCAATAA GTAAATTCAT GGAAAGGAAG 
AAATTAAAAG AAAGTGAGGA AAAGGAAGTG CTTCTGAAAA CAAACCTTTC TGGACGGCAG 
AGCCCAAGTT TCAAGCTTTC CCTGTCCAGT GGAACGAAGA CTAACCTCAC CAGCCAGTCA 
TCTACAACAA ATCTGCCTGG TTCTCCGGGA TCACCTGGAT CCCCAGGATC TCCAGGCTCT 
CCTGGATCCG TACCTAAAAA TACATCTCAG ACGGCAGCTA TTACTACAAA GGGAGGCCTC 
GTGGGTCTGG TAGATTATCC TGATGATGAT GAAGATGATG ATGAGGATGA AGATAAGGAA 
GATACGTTAC CATTGTCAAA GAAAGCAAAA TTTGATTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001353361.1
CDS	58..2559
Translation	MTDTRRRVKVYTLNEDRQWDDRGTGHVSSGYVERLKGMSLLVRAESDGSLLLESKINPNTAYQKQQDTLIVWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVDITQDLVDESEEERFDDMSSPGLELPSCELSRLEEIAELVASSLPSPLRREKLALALENEGYIKKLLELFHVCEDLENIEGLHHLYEIIKGIFLLNRTALFEVMFSEECIMDVIGCLEYDPALSQPRKHREFLTKTAKFKEVIPISDPELKQKIHQTYRVQYIQDMVLPTPSVFEENMLSTLHSFIFFNKVEIVGMLQEDEKFLTDLFAQLTDEATDEEKRQELVNFLKEFCAFSQTLQPQNRDAFFKTLSNMGILPALEVILGMDDTQVRSAATDIFSYLVEYNPSMVREFVMQEAQQNDDVSKKLTEQKITSKDILLINLIIEHMICDTDPELGGAVQLMGLLRTLVDPENMLATANKTEKTEFLGFFYKHCMHVLTAPLLANTTEDKPSKDDFQTAQLLALVLELLTFCVEHHTYHIKNYIINKDILRRVLVLMASKHAFLALCALRFKRKIIGLKDEFYNRYIMKSFLFEPVVKAFLNNGSRYNLMNSAIIEMFEFIRVEDIKSLTAHVIENYWKALEDVDYVQTFKGLKLRFEQQRERQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDDMEDGEAVVSPSDKTKNDDDIMDPISKFMERKKLKESEEKEVLLKTNLSGRQSPSFKLSLSSGTKTNLTSQSSTTNLPGSPGSPGSPGSPGSPGSVPKNTSQTAAITTKGGLVGLVDYPDDDEDDDEDEDKEDTLPLSKKAKFDS

Target ORF information:

RefSeq Version	NM_001366432.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 4 regulatory subunit 3A (PPP4R3A), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001366432.1

ORF Insert Sequence:

ATGACCGACA CCCGGCGGCG GGTGAAGGTG TACACGCTCA ACGAGGACCG GCAGTGGGAC 
GACCGGGGCA CCGGGCATGT GTCGTCTGGC TACGTGGAGC GGCTGAAGGG CATGTCCCTG 
CTTGTCAGGG CTGAGAGCGA CGGTTCTCTA CTTTTAGAGT CGAAAATAAA TCCTAACACT 
GCATACCAGA AACAACAGGA CACTCTGATT GTGTGGTCTG AAGCAGAAAA TTATGACTTG 
GCCCTTAGCT TTCAAGAAAA AGCTGGATGT GATGAAATTT GGGAGAAAAT ATGTCAGGTT 
CAAGGAAAGG ACCCTTCCGT GGACATCACT CAGGACCTTG TGGATGAATC TGAAGAGGAG 
CGTTTTGATG ATATGTCATC GCCAGGCTTA GAATTGCCAT CTTGTGAATT AAGTCGCCTT 
GAAGAAATTG CAGAACTTGT GGCATCATCT TTACCTTCAC CTCTTCGTCG TGAAAAACTT 
GCACTGGCAC TAGAAAATGA GGGTTATATT AAAAAGCTCC TGGAGCTTTT TCATGTGTGT 
GAAGATTTGG AAAATATTGA AGGACTGCAC CACTTGTATG AAATTATCAA AGGCATCTTT 
CTCTTGAATC GAACTGCTCT TTTTGAAGTT ATGTTCTCTG AAGAATGTAT AATGGACGTC 
ATTGGATGTT TAGAATATGA TCCTGCTTTA TCACAACCAC GAAAACACAG GGAATTTCTA 
ACAAAAACAG CCAAGTTTAA AGAAGTGATT CCCATATCAG ATCCTGAGCT GAAACAAAAA 
ATTCATCAGA CATACAGAGT TCAGTATATA CAAGATATGG TTCTACCAAC TCCTTCGGTC 
TTTGAAGAAA ACATGTTATC AACACTTCAC TCTTTTATCT TTTTCAATAA GGTAGAGATT 
GTTGGCATGT TGCAGGAAGA TGAAAAATTT CTGACAGATT TGTTTGCACA ACTAACAGAT 
GAAGCAACAG ATGAGGAAAA AAGACAGGAA TTGGTTAACT TTTTAAAAGA ATTTTGTGCG 
TTTTCCCAAA CGCTACAGCC TCAAAACAGA GATGCTTTTT TCAAGACTTT GTCAAACATG 
GGCATATTAC CAGCTTTAGA AGTCATCCTT GGCATGGATG ATACACAGGT GCGAAGTGCT 
GCTACTGATA TATTCTCATA CTTGGTTGAA TATAATCCAT CCATGGTACG AGAGTTTGTC 
ATGCAGGAGG CACAACAGAA TGATGATGTA AGTAAGAAGT TAACAGAGCA AAAAATAACC 
AGCAAGGATA TTTTGCTCAT CAACCTCATT ATAGAACATA TGATTTGTGA TACAGATCCT 
GAACTTGGAG GAGCAGTCCA GCTTATGGGC CTGCTTCGAA CTTTAGTTGA CCCAGAGAAC 
ATGCTAGCCA CTGCCAATAA AACAGAAAAG ACTGAATTTC TGGGTTTCTT CTACAAGCAC 
TGTATGCATG TTCTCACTGC TCCTTTACTA GCAAATACAA CAGAAGACAA ACCTAGTAAA 
GATGATTTTC AGACTGCCCA ACTATTGGCA CTTGTATTGG AATTGTTAAC ATTTTGTGTG 
GAGCACCATA CCTACCACAT AAAGAACTAC ATTATTAATA AGGATATCCT CCGGAGAGTG 
CTAGTTCTTA TGGCCTCGAA GCATGCTTTC TTGGCATTAT GTGCCCTTCG TTTTAAAAGA 
AAGATTATTG GATTAAAAGA TGAGTTTTAC AACCGCTACA TAATGAAAAG TTTTTTGTTT 
GAACCAGTAG TGAAAGCATT TCTCAACAAT GGATCCCGCT ACAATCTGAT GAACTCTGCC 
ATAATAGAGA TGTTTGAATT TATTAGAGTG GAAGATATAA AATCATTAAC TGCTCATGTA 
ATTGAAAATT ACTGGAAAGC ACTGGAAGAT GTAGATTATG TACAGACATT TAAAGGATTA 
AAACTGAGAT TTGAACAACA AAGAGAAAGG CAAGATAATC CCAAACTTGA CAGTATGCGT 
TCCATTTTGA GGAATCACAG ATATCGAAGA GATGCCAGAA CACTAGAAGA TGAAGAAGAG 
ATGTGGTTTA ACACAGATGA AGATGACATG GAAGATGGAG AAGCTGTAGT GTCTCCATCT 
GACAAAACTA AAAATGATGA TGATATTATG GATCCAATAA GTAAATTCAT GGAAAGGAAG 
AAATTAAAAG AAAGTGAGGA AAAGGAAGTG CTTCTGAAAA CAAACCTTTC TGGACGGCAG 
AGCCCAAGTT TCAAGCTTTC CCTGTCCAGT GGAACGAAGA CTAACCTCAC CAGCCAGTCA 
TCTACAACAA ATCTGCCTGG TTCTCCGGGA TCACCTGGAT CCCCAGGATC TCCAGGCTCT 
CCTGGATCCG TACCTAAAAA TACATCTCAG ACGGCAGCTA TTACTACAAA GGGAGGCCTC 
GTGGGTCTGG TAGATTATCC TGATGATGAT GAAGATGATG ATGAGGATGA AGATAAGGAA 
GATACGTTAC CATTGTCAAA GAAAGCAAAA TTTGATTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP4R3A ( XM_005267842.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005267899.1 Homo sapiens protein phosphatase 4 regulatory subunit 3A (PPP4R3A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu47788
Clone ID Related Accession (Same CDS sequence)	NM_001366432.1 , XM_005267842.2
Accession Version	XM_005267842.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 4 regulatory subunit 3A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005267842.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGACA CCCGGCGGCG GGTGAAGGTG TACACGCTCA ACGAGGACCG GCAGTGGGAC 
GACCGGGGCA CCGGGCATGT GTCGTCTGGC TACGTGGAGC GGCTGAAGGG CATGTCCCTG 
CTTGTCAGGG CTGAGAGCGA CGGTTCTCTA CTTTTAGAGT CGAAAATAAA TCCTAACACT 
GCATACCAGA AACAACAGGA CACTCTGATT GTGTGGTCTG AAGCAGAAAA TTATGACTTG 
GCCCTTAGCT TTCAAGAAAA AGCTGGATGT GATGAAATTT GGGAGAAAAT ATGTCAGGTT 
CAAGGAAAGG ACCCTTCCGT GGACATCACT CAGGACCTTG TGGATGAATC TGAAGAGGAG 
CGTTTTGATG ATATGTCATC GCCAGGCTTA GAATTGCCAT CTTGTGAATT AAGTCGCCTT 
GAAGAAATTG CAGAACTTGT GGCATCATCT TTACCTTCAC CTCTTCGTCG TGAAAAACTT 
GCACTGGCAC TAGAAAATGA GGGTTATATT AAAAAGCTCC TGGAGCTTTT TCATGTGTGT 
GAAGATTTGG AAAATATTGA AGGACTGCAC CACTTGTATG AAATTATCAA AGGCATCTTT 
CTCTTGAATC GAACTGCTCT TTTTGAAGTT ATGTTCTCTG AAGAATGTAT AATGGACGTC 
ATTGGATGTT TAGAATATGA TCCTGCTTTA TCACAACCAC GAAAACACAG GGAATTTCTA 
ACAAAAACAG CCAAGTTTAA AGAAGTGATT CCCATATCAG ATCCTGAGCT GAAACAAAAA 
ATTCATCAGA CATACAGAGT TCAGTATATA CAAGATATGG TTCTACCAAC TCCTTCGGTC 
TTTGAAGAAA ACATGTTATC AACACTTCAC TCTTTTATCT TTTTCAATAA GGTAGAGATT 
GTTGGCATGT TGCAGGAAGA TGAAAAATTT CTGACAGATT TGTTTGCACA ACTAACAGAT 
GAAGCAACAG ATGAGGAAAA AAGACAGGAA TTGGTTAACT TTTTAAAAGA ATTTTGTGCG 
TTTTCCCAAA CGCTACAGCC TCAAAACAGA GATGCTTTTT TCAAGACTTT GTCAAACATG 
GGCATATTAC CAGCTTTAGA AGTCATCCTT GGCATGGATG ATACACAGGT GCGAAGTGCT 
GCTACTGATA TATTCTCATA CTTGGTTGAA TATAATCCAT CCATGGTACG AGAGTTTGTC 
ATGCAGGAGG CACAACAGAA TGATGATGTA AGTAAGAAGT TAACAGAGCA AAAAATAACC 
AGCAAGGATA TTTTGCTCAT CAACCTCATT ATAGAACATA TGATTTGTGA TACAGATCCT 
GAACTTGGAG GAGCAGTCCA GCTTATGGGC CTGCTTCGAA CTTTAGTTGA CCCAGAGAAC 
ATGCTAGCCA CTGCCAATAA AACAGAAAAG ACTGAATTTC TGGGTTTCTT CTACAAGCAC 
TGTATGCATG TTCTCACTGC TCCTTTACTA GCAAATACAA CAGAAGACAA ACCTAGTAAA 
GATGATTTTC AGACTGCCCA ACTATTGGCA CTTGTATTGG AATTGTTAAC ATTTTGTGTG 
GAGCACCATA CCTACCACAT AAAGAACTAC ATTATTAATA AGGATATCCT CCGGAGAGTG 
CTAGTTCTTA TGGCCTCGAA GCATGCTTTC TTGGCATTAT GTGCCCTTCG TTTTAAAAGA 
AAGATTATTG GATTAAAAGA TGAGTTTTAC AACCGCTACA TAATGAAAAG TTTTTTGTTT 
GAACCAGTAG TGAAAGCATT TCTCAACAAT GGATCCCGCT ACAATCTGAT GAACTCTGCC 
ATAATAGAGA TGTTTGAATT TATTAGAGTG GAAGATATAA AATCATTAAC TGCTCATGTA 
ATTGAAAATT ACTGGAAAGC ACTGGAAGAT GTAGATTATG TACAGACATT TAAAGGATTA 
AAACTGAGAT TTGAACAACA AAGAGAAAGG CAAGATAATC CCAAACTTGA CAGTATGCGT 
TCCATTTTGA GGAATCACAG ATATCGAAGA GATGCCAGAA CACTAGAAGA TGAAGAAGAG 
ATGTGGTTTA ACACAGATGA AGATGACATG GAAGATGGAG AAGCTGTAGT GTCTCCATCT 
GACAAAACTA AAAATGATGA TGATATTATG GATCCAATAA GTAAATTCAT GGAAAGGAAG 
AAATTAAAAG AAAGTGAGGA AAAGGAAGTG CTTCTGAAAA CAAACCTTTC TGGACGGCAG 
AGCCCAAGTT TCAAGCTTTC CCTGTCCAGT GGAACGAAGA CTAACCTCAC CAGCCAGTCA 
TCTACAACAA ATCTGCCTGG TTCTCCGGGA TCACCTGGAT CCCCAGGATC TCCAGGCTCT 
CCTGGATCCG TACCTAAAAA TACATCTCAG ACGGCAGCTA TTACTACAAA GGGAGGCCTC 
GTGGGTCTGG TAGATTATCC TGATGATGAT GAAGATGATG ATGAGGATGA AGATAAGGAA 
GATACGTTAC CATTGTCAAA GAAAGCAAAA TTTGATTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005267899.1
CDS	84..2585
Translation	MTDTRRRVKVYTLNEDRQWDDRGTGHVSSGYVERLKGMSLLVRAESDGSLLLESKINPNTAYQKQQDTLIVWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVDITQDLVDESEEERFDDMSSPGLELPSCELSRLEEIAELVASSLPSPLRREKLALALENEGYIKKLLELFHVCEDLENIEGLHHLYEIIKGIFLLNRTALFEVMFSEECIMDVIGCLEYDPALSQPRKHREFLTKTAKFKEVIPISDPELKQKIHQTYRVQYIQDMVLPTPSVFEENMLSTLHSFIFFNKVEIVGMLQEDEKFLTDLFAQLTDEATDEEKRQELVNFLKEFCAFSQTLQPQNRDAFFKTLSNMGILPALEVILGMDDTQVRSAATDIFSYLVEYNPSMVREFVMQEAQQNDDVSKKLTEQKITSKDILLINLIIEHMICDTDPELGGAVQLMGLLRTLVDPENMLATANKTEKTEFLGFFYKHCMHVLTAPLLANTTEDKPSKDDFQTAQLLALVLELLTFCVEHHTYHIKNYIINKDILRRVLVLMASKHAFLALCALRFKRKIIGLKDEFYNRYIMKSFLFEPVVKAFLNNGSRYNLMNSAIIEMFEFIRVEDIKSLTAHVIENYWKALEDVDYVQTFKGLKLRFEQQRERQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDDMEDGEAVVSPSDKTKNDDDIMDPISKFMERKKLKESEEKEVLLKTNLSGRQSPSFKLSLSSGTKTNLTSQSSTTNLPGSPGSPGSPGSPGSPGSVPKNTSQTAAITTKGGLVGLVDYPDDDEDDDEDEDKEDTLPLSKKAKFDS

Target ORF information:

RefSeq Version	XM_005267842.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 4 regulatory subunit 3A (PPP4R3A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005267842.2

ORF Insert Sequence:

ATGACCGACA CCCGGCGGCG GGTGAAGGTG TACACGCTCA ACGAGGACCG GCAGTGGGAC 
GACCGGGGCA CCGGGCATGT GTCGTCTGGC TACGTGGAGC GGCTGAAGGG CATGTCCCTG 
CTTGTCAGGG CTGAGAGCGA CGGTTCTCTA CTTTTAGAGT CGAAAATAAA TCCTAACACT 
GCATACCAGA AACAACAGGA CACTCTGATT GTGTGGTCTG AAGCAGAAAA TTATGACTTG 
GCCCTTAGCT TTCAAGAAAA AGCTGGATGT GATGAAATTT GGGAGAAAAT ATGTCAGGTT 
CAAGGAAAGG ACCCTTCCGT GGACATCACT CAGGACCTTG TGGATGAATC TGAAGAGGAG 
CGTTTTGATG ATATGTCATC GCCAGGCTTA GAATTGCCAT CTTGTGAATT AAGTCGCCTT 
GAAGAAATTG CAGAACTTGT GGCATCATCT TTACCTTCAC CTCTTCGTCG TGAAAAACTT 
GCACTGGCAC TAGAAAATGA GGGTTATATT AAAAAGCTCC TGGAGCTTTT TCATGTGTGT 
GAAGATTTGG AAAATATTGA AGGACTGCAC CACTTGTATG AAATTATCAA AGGCATCTTT 
CTCTTGAATC GAACTGCTCT TTTTGAAGTT ATGTTCTCTG AAGAATGTAT AATGGACGTC 
ATTGGATGTT TAGAATATGA TCCTGCTTTA TCACAACCAC GAAAACACAG GGAATTTCTA 
ACAAAAACAG CCAAGTTTAA AGAAGTGATT CCCATATCAG ATCCTGAGCT GAAACAAAAA 
ATTCATCAGA CATACAGAGT TCAGTATATA CAAGATATGG TTCTACCAAC TCCTTCGGTC 
TTTGAAGAAA ACATGTTATC AACACTTCAC TCTTTTATCT TTTTCAATAA GGTAGAGATT 
GTTGGCATGT TGCAGGAAGA TGAAAAATTT CTGACAGATT TGTTTGCACA ACTAACAGAT 
GAAGCAACAG ATGAGGAAAA AAGACAGGAA TTGGTTAACT TTTTAAAAGA ATTTTGTGCG 
TTTTCCCAAA CGCTACAGCC TCAAAACAGA GATGCTTTTT TCAAGACTTT GTCAAACATG 
GGCATATTAC CAGCTTTAGA AGTCATCCTT GGCATGGATG ATACACAGGT GCGAAGTGCT 
GCTACTGATA TATTCTCATA CTTGGTTGAA TATAATCCAT CCATGGTACG AGAGTTTGTC 
ATGCAGGAGG CACAACAGAA TGATGATGTA AGTAAGAAGT TAACAGAGCA AAAAATAACC 
AGCAAGGATA TTTTGCTCAT CAACCTCATT ATAGAACATA TGATTTGTGA TACAGATCCT 
GAACTTGGAG GAGCAGTCCA GCTTATGGGC CTGCTTCGAA CTTTAGTTGA CCCAGAGAAC 
ATGCTAGCCA CTGCCAATAA AACAGAAAAG ACTGAATTTC TGGGTTTCTT CTACAAGCAC 
TGTATGCATG TTCTCACTGC TCCTTTACTA GCAAATACAA CAGAAGACAA ACCTAGTAAA 
GATGATTTTC AGACTGCCCA ACTATTGGCA CTTGTATTGG AATTGTTAAC ATTTTGTGTG 
GAGCACCATA CCTACCACAT AAAGAACTAC ATTATTAATA AGGATATCCT CCGGAGAGTG 
CTAGTTCTTA TGGCCTCGAA GCATGCTTTC TTGGCATTAT GTGCCCTTCG TTTTAAAAGA 
AAGATTATTG GATTAAAAGA TGAGTTTTAC AACCGCTACA TAATGAAAAG TTTTTTGTTT 
GAACCAGTAG TGAAAGCATT TCTCAACAAT GGATCCCGCT ACAATCTGAT GAACTCTGCC 
ATAATAGAGA TGTTTGAATT TATTAGAGTG GAAGATATAA AATCATTAAC TGCTCATGTA 
ATTGAAAATT ACTGGAAAGC ACTGGAAGAT GTAGATTATG TACAGACATT TAAAGGATTA 
AAACTGAGAT TTGAACAACA AAGAGAAAGG CAAGATAATC CCAAACTTGA CAGTATGCGT 
TCCATTTTGA GGAATCACAG ATATCGAAGA GATGCCAGAA CACTAGAAGA TGAAGAAGAG 
ATGTGGTTTA ACACAGATGA AGATGACATG GAAGATGGAG AAGCTGTAGT GTCTCCATCT 
GACAAAACTA AAAATGATGA TGATATTATG GATCCAATAA GTAAATTCAT GGAAAGGAAG 
AAATTAAAAG AAAGTGAGGA AAAGGAAGTG CTTCTGAAAA CAAACCTTTC TGGACGGCAG 
AGCCCAAGTT TCAAGCTTTC CCTGTCCAGT GGAACGAAGA CTAACCTCAC CAGCCAGTCA 
TCTACAACAA ATCTGCCTGG TTCTCCGGGA TCACCTGGAT CCCCAGGATC TCCAGGCTCT 
CCTGGATCCG TACCTAAAAA TACATCTCAG ACGGCAGCTA TTACTACAAA GGGAGGCCTC 
GTGGGTCTGG TAGATTATCC TGATGATGAT GAAGATGATG ATGAGGATGA AGATAAGGAA 
GATACGTTAC CATTGTCAAA GAAAGCAAAA TTTGATTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A variant in PPP4R3A protects against alzheimer-related metabolic decline.
Annals of neurology82(6)900-911(2017 Dec)
Christopher L,Napolioni V,Khan RR,Han SS,Greicius MD,

BMI-1 interacts with sMEK1 and inactivates sMEK1-induced apoptotic cell death.
Oncology reports37(1)579-586(2017 Jan)
Kim BR,Kwon Y,Rho SB

sMEK1 inhibits endothelial cell proliferation by attenuating VEGFR-2-dependent-Akt/eNOS/HIF-1? signaling pathways.
Oncotarget6(31)31830-43(2015 Oct)
Kim BR,Seo SH,Park MS,Lee SH,Kwon Y,Rho SB

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M

Tumor suppressor BLU enhances pro-apoptotic activity of sMEK1 through physical interaction.
Cellular signalling24(6)1208-14(2012 Jun)
Dong SM,Byun HJ,Kim BR,Lee SH,Trink B,Rho SB

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PPP4R3A cDNA ORF clone, Homo sapiens(human)

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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