Ppp4r3a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppp4r3a
Entrez Gene ID	68734
Full Name	protein phosphatase 4 regulatory subunit 3A
Synonyms	1110034C04Rik,BC064465,Smek1,mKIAA2010
General protein information	Preferred Names protein phosphatase 4 regulatory subunit 3A Names serine/threonine-protein phosphatase 4 regulatory subunit 3A SMEK homolog 1, suppressor of mek1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 E

mRNA and Protein(s)

mRNA	Protein	Name
XM_017315174.2	XP_017170663.1	serine/threonine-protein phosphatase 4 regulatory subunit 3A isoform X2
XM_017315174.1	XP_017170663.1	serine/threonine-protein phosphatase 4 regulatory subunit 3A isoform X2
NM_001160214.1	NP_001153686.1	serine/threonine-protein phosphatase 4 regulatory subunit 3A isoform 2
XM_017315173.1	XP_017170662.1	serine/threonine-protein phosphatase 4 regulatory subunit 3A isoform X1
NM_211355.2	NP_997594.1	serine/threonine-protein phosphatase 4 regulatory subunit 3A isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SMEK1	NP_001271209.1
Pan troglodytes (chimpanzee)	SMEK1	XP_001140788.2
Bos taurus (cattle)	SMEK1	NP_001192449.1
Arabidopsis thaliana (thale cress)	AT3G06670	NP_001189831.1
Canis lupus familiaris (dog)	SMEK1	XP_005623804.1
Magnaporthe oryzae (rice blast fungus)	MGG_06138	XP_003712012.1
Xenopus tropicalis (tropical clawed frog)	LOC100485426	XP_002933248.1
Mus musculus (house mouse)	Smek1	NP_001153686.1
Danio rerio (zebrafish)	smek1	NP_001038274.1
Caenorhabditis elegans (roundworm)	smk-1	NP_001023909.2
Schizosaccharomyces pombe (fission yeast)	SPBC216.01c	XP_001713123.1
Rattus norvegicus (Norway rat)	Smek1	NP_001258103.1
Gallus gallus (chicken)	SMEK1	XP_421321.2
Neurospora crassa	NCU11315	XP_001728203.1
Oryza sativa (rice)	Os01g0337600	NP_001042939.2

Gene Ontology
Bibliography

Related articles in PubMed

Smek1/2 is a nuclear chaperone and cofactor for cleaved Wnt receptor Ryk, regulating cortical neurogenesis.
Chang WH, Choi SH, Moon BS, Cai M, Lyu J, Bai J, Gao F, Hajjali I, Zhao Z, Campbell DB, Weiner LP, Lu W
Proceedings of the National Academy of Sciences of the United States of America114(50)E10717-E10725(2017 Dec)

Smek promotes corticogenesis through regulating Mbd3's stability and Mbd3/NuRD complex recruitment to genes associated with neurogenesis.
Moon BS, Yun HM, Chang WH, Steele BH, Cai M, Choi SH, Lu W
PLoS biology15(5)e2001220(2017 May)

Protein phosphatase 4 and Smek complex negatively regulate Par3 and promote neuronal differentiation of neural stem/progenitor cells.
Lyu J, Kim HR, Yamamoto V, Choi SH, Wei Z, Joo CK, Lu W
Cell reports5(3)593-600(2013 Nov)

Smek promotes histone deacetylation to suppress transcription of Wnt target gene brachyury in pluripotent embryonic stem cells.
Lyu J, Jho EH, Lu W
Cell research21(6)911-21(2011 Jun)

Suppressor of MEK null (SMEK)/protein phosphatase 4 catalytic subunit (PP4C) is a key regulator of hepatic gluconeogenesis.
Yoon YS, Lee MW, Ryu D, Kim JH, Ma H, Seo WY, Kim YN, Kim SS, Lee CH, Hunter T, Choi CS, Montminy MR, Koo SH
Proceedings of the National Academy of Sciences of the United States of America107(41)17704-9(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Smek/Mbd3 interaction regulates neuronal gene expression and neuronal differentiation during cortical development.
Title: Smek promotes corticogenesis through regulating Mbd3's stability and Mbd3/NuRD complex recruitment to genes associated with neurogenesis.

Authors identify the cell polarity protein Par3, a negative regulator of neuronal differentiation, as a Smek1 substrate and demonstrate that Smek1 suppresses its activity.
Title: Protein phosphatase 4 and Smek complex negatively regulate Par3 and promote neuronal differentiation of neural stem/progenitor cells.

the Smek-containing protein phosphatase 4 complex represses transcription of Wnt-responsive development-related genes through histone deacetylation, and that this complex is essential for embryonic stem cell pluripotency maintenance
Title: Smek promotes histone deacetylation to suppress transcription of Wnt target gene brachyury in pluripotent embryonic stem cells.

The following Ppp4r3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppp4r3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15780	NM_001160214.1 Latest version!	Mus musculus protein phosphatase 4 regulatory subunit 3A (Ppp4r3a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$489.30 $699.00
OMu15380	NM_211355.2 Latest version!	Mus musculus protein phosphatase 4 regulatory subunit 3A (Ppp4r3a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$482.30 $689.00
OMu78290	XM_017315173.1 Latest version!	Mus musculus protein phosphatase 4 regulatory subunit 3A (Ppp4r3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu78291	XM_017315174.2 Latest version!	Mus musculus protein phosphatase 4 regulatory subunit 3A (Ppp4r3a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu78291	XM_017315174.1	Mus musculus SMEK homolog 1, suppressor of mek1 (Dictyostelium) (Smek1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppp4r3a ( NM_001160214.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001153686.1 Mus musculus protein phosphatase 4 regulatory subunit 3A (Ppp4r3a), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu15780
Clone ID Related Accession (Same CDS sequence)	NM_001160214.1
Accession Version	NM_001160214.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein phosphatase 4 regulatory subunit 3A isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK053668.1, BC064465.1, AK152480.1 and AK054087.1. Transcript Variant: This variant (2) uses an alternate in-frame donor splice site at one of the coding exons compared to variant 1, resulting in a longer isoform (2) with an internal protein segment not found in isoform 1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164133, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCGACA CCCGACGGCG GGTGAAGGTC TACACGCTCA ACGAGGACCG GCAGTGGGAC 
GACCGGGGTA CTGGCCATGT GTCCTCCGGC TACGTGGAGA GGCTGAAGGG CATGTCCCTG 
CTCGTCAGGG CGGAGAGCGA CGGTTCTCTT CTTCTAGAGT CCAAAATAAA TCCTAACACT 
GCATACCAGA AACAACAGGA TACTCTGATT GTGTGGTCTG AAGCAGAAAA TTATGACTTG 
GCCCTTAGCT TTCAAGAAAA AGCTGGATGC GATGAAATTT GGGAGAAAAT ATGTCAGGTT 
CAAGGAAAGG ACCCTTCAGT GGACATCACT CAGGACCTGG TAGATGAATC TGAAGAGGAG 
CGATTTGATG ATATGTCCTC ACCAGGTTTA GAATTGCCGT CATGTGAGTT AAGTCGACTT 
GAGGAAATTG CAGAGCTTGT GGCATCATCT TTACCTTCCC CCCTCCGACG TGAGAAACTC 
GCACTAGCAC TGGAAAATGA GGGTTATATT AAAAAGCTTT TAGAGCTTTT TCATGTGTGT 
GAGGATTTGG AAAACATTGA AGGACTGCAC CACTTATATG AAATTATTAA AGGGATCTTC 
CTCTTGAATC GAACTGCTCT TTTTGAAGTT ATGTTCTCTG AGGAATGTAT AATGGACGTC 
ATTGGATGCT TGGAATATGA CCCAACTTTA TCACAACCAC GTAAACACAG GGAATTTTTA 
ACAAAAACAG CCAAGTTTAA AGAAGTAATT CCCATATCAG ATCCTGAGCT GAAACAAAAA 
ATTCATCAGA CATACAGAGT TCAGTATATA CAAGATATGG TTCTCCCTAC CCCTTCAGTC 
TTCGAAGAAA ACATGTTATC AACACTTCAC TCCTTTATCT TTTTCAACAA GGTAGAAATC 
GTTGGTATGT TACAGGAAGA TGAAAAATTT CTGACAGATT TGTTTGCACA ACTAACAGAT 
GAAGCAACAG ATGAGGAAAA AAGACAAGAA TTGGTTAACT TTTTAAAAGA ATTTTGTGCA 
TTTTCTCAAA CGCTACAACC CCAAAACAGA GATGCTTTTT TCAAGACTTT GTCAAATATG 
GGCATATTAC CAGCTTTAGA AGTCATCCTT GGCATGGATG ATACACAGGT GCGAAGTGCT 
GCTACGGATA TATTCTCATA CTTGGTTGAA TATAATCCAT CCATGGTACG AGAGTTTGTC 
ATGCAGGAGG CACAACAGAA TGATGATGTA AGTAAGAAGT TAACAGAGCA AAAAATAACC 
AGCAAGGATA TTTTGCTCAT CAACCTCATT ATAGAACATA TGATTTGTGA TACAGATCCC 
GAGCTTGGAG GGGCAGTCCA ACTTATGGGC CTGCTTCGAA CTTTAGTTGA CCCAGAGAAC 
ATGCTAGCCA CTGCCAATAA AACAGAAAAG ACTGAATTTC TGGGTTTCTT CTACAAGCAC 
TGTATGCATG TTCTCACTGC TCCCTTACTA GCAAATACAA CAGAAGACAA GCCTAGTAAA 
GATGATTTTC AAACTGCGCA GTTACTAGCA CTTGTATTGG AATTGCTAAC ATTTTGTGTG 
GAGCACCATA CCTACCACAT AAAGAACTAT ATTATCAACA AGGACATTCT TCGCAGAGTG 
CTAGTTCTTA TGGCCTCCAA GCATGCTTTC TTGGCATTAT GTGCCCTCCG TTTTAAGAGA 
AAAATCATTG GATTAAAAGA TGAATTTTAT AACCGCTACA TAATGAAAAG TTTTTTATTT 
GAACCTGTAG TCAAAGCATT TCTCAACAAC GGATCTCGTT ACAATCTGAT GAACTCCGCC 
ATAATAGAAA TGTTTGAATT TATTAGAGTG GAAGATATAA AATCATTAAC TGCCCATGTA 
ATTGAGAATT ATTGGAAAGC ACTAGAAGAT GTAGATTATG TACAGACGTT TAAAGGATTA 
AAATTGAGGT TTGAACAGCA AAGAGAAAGG CAAGACAACC CCAAACTTGA TAGCATGCGG 
TCTATCTTGA GGAATCATCG ATATCGACGA GATGCCAGAA CACTAGAAGA CGAAGAAGAG 
ATGTGGTTCA ACACAGATGA AGATGACATG GAAGATGGAG AAGCTGTGGT GTCTCCATCT 
GACAAAACGA AGAATGACGA TGATATTATG GATCCAATAA GTAAATTTAT GGAAAGGAAG 
AAATTGAAAG AAAGTGAAGA GAAGGAGGTG CTTCTGAAAA CTAATCTTTC TGGACGACAG 
AGCCCAAGTT TCAAACTTTC CTTGTCCAGT GGGACAAAGA CTAACCTCAC CAGCCAGTCA 
TCCGCAACAA GCCTGCCTGG TTCTCCAGGG TCACCTGGCT CCCCGGGCTC TCCGGGCTCT 
CCTGGATCCG TCCCTAAGAG TACATCTCAG ACGGCAGCTA TTACCACAAA GGGAGGACTC 
GTGGGTCTGG TAGATTATCC TGATGATGAT GAAGATGATG ATGAGGATGA AGACAAGGAA 
GACACACTAC CAGTGTCAAA GAAAGCAAAA TTTGAGTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153686.1
CDS	833..3334
Translation	MTDTRRRVKVYTLNEDRQWDDRGTGHVSSGYVERLKGMSLLVRAESDGSLLLESKINPNTAYQKQQDTLIVWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVDITQDLVDESEEERFDDMSSPGLELPSCELSRLEEIAELVASSLPSPLRREKLALALENEGYIKKLLELFHVCEDLENIEGLHHLYEIIKGIFLLNRTALFEVMFSEECIMDVIGCLEYDPTLSQPRKHREFLTKTAKFKEVIPISDPELKQKIHQTYRVQYIQDMVLPTPSVFEENMLSTLHSFIFFNKVEIVGMLQEDEKFLTDLFAQLTDEATDEEKRQELVNFLKEFCAFSQTLQPQNRDAFFKTLSNMGILPALEVILGMDDTQVRSAATDIFSYLVEYNPSMVREFVMQEAQQNDDVSKKLTEQKITSKDILLINLIIEHMICDTDPELGGAVQLMGLLRTLVDPENMLATANKTEKTEFLGFFYKHCMHVLTAPLLANTTEDKPSKDDFQTAQLLALVLELLTFCVEHHTYHIKNYIINKDILRRVLVLMASKHAFLALCALRFKRKIIGLKDEFYNRYIMKSFLFEPVVKAFLNNGSRYNLMNSAIIEMFEFIRVEDIKSLTAHVIENYWKALEDVDYVQTFKGLKLRFEQQRERQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDDMEDGEAVVSPSDKTKNDDDIMDPISKFMERKKLKESEEKEVLLKTNLSGRQSPSFKLSLSSGTKTNLTSQSSATSLPGSPGSPGSPGSPGSPGSVPKSTSQTAAITTKGGLVGLVDYPDDDEDDDEDEDKEDTLPVSKKAKFES

Target ORF information:

RefSeq Version	NM_001160214.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 4 regulatory subunit 3A (Ppp4r3a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160214.1

ORF Insert Sequence:

ATGACCGACA CCCGACGGCG GGTGAAGGTC TACACGCTCA ACGAGGACCG GCAGTGGGAC 
GACCGGGGTA CTGGCCATGT GTCCTCCGGC TACGTGGAGA GGCTGAAGGG CATGTCCCTG 
CTCGTCAGGG CGGAGAGCGA CGGTTCTCTT CTTCTAGAGT CCAAAATAAA TCCTAACACT 
GCATACCAGA AACAACAGGA TACTCTGATT GTGTGGTCTG AAGCAGAAAA TTATGACTTG 
GCCCTTAGCT TTCAAGAAAA AGCTGGATGC GATGAAATTT GGGAGAAAAT ATGTCAGGTT 
CAAGGAAAGG ACCCTTCAGT GGACATCACT CAGGACCTGG TAGATGAATC TGAAGAGGAG 
CGATTTGATG ATATGTCCTC ACCAGGTTTA GAATTGCCGT CATGTGAGTT AAGTCGACTT 
GAGGAAATTG CAGAGCTTGT GGCATCATCT TTACCTTCCC CCCTCCGACG TGAGAAACTC 
GCACTAGCAC TGGAAAATGA GGGTTATATT AAAAAGCTTT TAGAGCTTTT TCATGTGTGT 
GAGGATTTGG AAAACATTGA AGGACTGCAC CACTTATATG AAATTATTAA AGGGATCTTC 
CTCTTGAATC GAACTGCTCT TTTTGAAGTT ATGTTCTCTG AGGAATGTAT AATGGACGTC 
ATTGGATGCT TGGAATATGA CCCAACTTTA TCACAACCAC GTAAACACAG GGAATTTTTA 
ACAAAAACAG CCAAGTTTAA AGAAGTAATT CCCATATCAG ATCCTGAGCT GAAACAAAAA 
ATTCATCAGA CATACAGAGT TCAGTATATA CAAGATATGG TTCTCCCTAC CCCTTCAGTC 
TTCGAAGAAA ACATGTTATC AACACTTCAC TCCTTTATCT TTTTCAACAA GGTAGAAATC 
GTTGGTATGT TACAGGAAGA TGAAAAATTT CTGACAGATT TGTTTGCACA ACTAACAGAT 
GAAGCAACAG ATGAGGAAAA AAGACAAGAA TTGGTTAACT TTTTAAAAGA ATTTTGTGCA 
TTTTCTCAAA CGCTACAACC CCAAAACAGA GATGCTTTTT TCAAGACTTT GTCAAATATG 
GGCATATTAC CAGCTTTAGA AGTCATCCTT GGCATGGATG ATACACAGGT GCGAAGTGCT 
GCTACGGATA TATTCTCATA CTTGGTTGAA TATAATCCAT CCATGGTACG AGAGTTTGTC 
ATGCAGGAGG CACAACAGAA TGATGATGTA AGTAAGAAGT TAACAGAGCA AAAAATAACC 
AGCAAGGATA TTTTGCTCAT CAACCTCATT ATAGAACATA TGATTTGTGA TACAGATCCC 
GAGCTTGGAG GGGCAGTCCA ACTTATGGGC CTGCTTCGAA CTTTAGTTGA CCCAGAGAAC 
ATGCTAGCCA CTGCCAATAA AACAGAAAAG ACTGAATTTC TGGGTTTCTT CTACAAGCAC 
TGTATGCATG TTCTCACTGC TCCCTTACTA GCAAATACAA CAGAAGACAA GCCTAGTAAA 
GATGATTTTC AAACTGCGCA GTTACTAGCA CTTGTATTGG AATTGCTAAC ATTTTGTGTG 
GAGCACCATA CCTACCACAT AAAGAACTAT ATTATCAACA AGGACATTCT TCGCAGAGTG 
CTAGTTCTTA TGGCCTCCAA GCATGCTTTC TTGGCATTAT GTGCCCTCCG TTTTAAGAGA 
AAAATCATTG GATTAAAAGA TGAATTTTAT AACCGCTACA TAATGAAAAG TTTTTTATTT 
GAACCTGTAG TCAAAGCATT TCTCAACAAC GGATCTCGTT ACAATCTGAT GAACTCCGCC 
ATAATAGAAA TGTTTGAATT TATTAGAGTG GAAGATATAA AATCATTAAC TGCCCATGTA 
ATTGAGAATT ATTGGAAAGC ACTAGAAGAT GTAGATTATG TACAGACGTT TAAAGGATTA 
AAATTGAGGT TTGAACAGCA AAGAGAAAGG CAAGACAACC CCAAACTTGA TAGCATGCGG 
TCTATCTTGA GGAATCATCG ATATCGACGA GATGCCAGAA CACTAGAAGA CGAAGAAGAG 
ATGTGGTTCA ACACAGATGA AGATGACATG GAAGATGGAG AAGCTGTGGT GTCTCCATCT 
GACAAAACGA AGAATGACGA TGATATTATG GATCCAATAA GTAAATTTAT GGAAAGGAAG 
AAATTGAAAG AAAGTGAAGA GAAGGAGGTG CTTCTGAAAA CTAATCTTTC TGGACGACAG 
AGCCCAAGTT TCAAACTTTC CTTGTCCAGT GGGACAAAGA CTAACCTCAC CAGCCAGTCA 
TCCGCAACAA GCCTGCCTGG TTCTCCAGGG TCACCTGGCT CCCCGGGCTC TCCGGGCTCT 
CCTGGATCCG TCCCTAAGAG TACATCTCAG ACGGCAGCTA TTACCACAAA GGGAGGACTC 
GTGGGTCTGG TAGATTATCC TGATGATGAT GAAGATGATG ATGAGGATGA AGACAAGGAA 
GACACACTAC CAGTGTCAAA GAAAGCAAAA TTTGAGTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppp4r3a ( NM_211355.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_997594.1 Mus musculus protein phosphatase 4 regulatory subunit 3A (Ppp4r3a), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu15380
Clone ID Related Accession (Same CDS sequence)	NM_211355.2
Accession Version	NM_211355.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein phosphatase 4 regulatory subunit 3A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK053668.1, BC064465.1 and AK054087.1. On May 15, 2009 this sequence version replaced NM_211355.1. Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK156272.1, SRR1660821.193956.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164133, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCGACA CCCGACGGCG GGTGAAGGTC TACACGCTCA ACGAGGACCG GCAGTGGGAC 
GACCGGGGTA CTGGCCATGT GTCCTCCGGC TACGTGGAGA GGCTGAAGGG CATGTCCCTG 
CTCGTCAGGG CGGAGAGCGA CGGTTCTCTT CTTCTAGAGT CCAAAATAAA TCCTAACACT 
GCATACCAGA AACAACAGGA TACTCTGATT GTGTGGTCTG AAGCAGAAAA TTATGACTTG 
GCCCTTAGCT TTCAAGAAAA AGCTGGATGC GATGAAATTT GGGAGAAAAT ATGTCAGGTT 
CAAGGAAAGG ACCCTTCAGT GGACATCACT CAGGACCTGG TAGATGAATC TGAAGAGGAG 
CGATTTGATG ATATGTCCTC ACCAGGTTTA GAATTGCCGT CATGTGAGTT AAGTCGACTT 
GAGGAAATTG CAGAGCTTGT GGCATCATCT TTACCTTCCC CCCTCCGACG TGAGAAACTC 
GCACTAGCAC TGGAAAATGA GGGTTATATT AAAAAGCTTT TAGAGCTTTT TCATGTGTGT 
GAGGATTTGG AAAACATTGA AGGACTGCAC CACTTATATG AAATTATTAA AGGGATCTTC 
CTCTTGAATC GAACTGCTCT TTTTGAAGTT ATGTTCTCTG AGGAATGTAT AATGGACGTC 
ATTGGATGCT TGGAATATGA CCCAACTTTA TCACAACCAC GTAAACACAG GGAATTTTTA 
ACAAAAACAG CCAAGTTTAA AGAAGTAATT CCCATATCAG ATCCTGAGCT GAAACAAAAA 
ATTCATCAGA CATACAGAGT TCAGTATATA CAAGATATGG TTCTCCCTAC CCCTTCAGTC 
TTCGAAGAAA ACATGTTATC AACACTTCAC TCCTTTATCT TTTTCAACAA GGTAGAAATC 
GTTGGTATGT TACAGGAAGA TGAAAAATTT CTGACAGATT TGTTTGCACA ACTAACAGAT 
GAAGCAACAG ATGAGGAAAA AAGACAAGAA TTGGTTAACT TTTTAAAAGA ATTTTGTGCA 
TTTTCTCAAA CGCTACAACC CCAAAACAGA GATGCTTTTT TCAAGACTTT GTCAAATATG 
GGCATATTAC CAGCTTTAGA AGTCATCCTT GGCATGGATG ATACACAGGT GCGAAGTGCT 
GCTACGGATA TATTCTCATA CTTGGTTGAA TATAATCCAT CCATGGTACG AGAGTTTGTC 
ATGCAGGAGG CACAACAGAA TGATGATGAT ATTTTGCTCA TCAACCTCAT TATAGAACAT 
ATGATTTGTG ATACAGATCC CGAGCTTGGA GGGGCAGTCC AACTTATGGG CCTGCTTCGA 
ACTTTAGTTG ACCCAGAGAA CATGCTAGCC ACTGCCAATA AAACAGAAAA GACTGAATTT 
CTGGGTTTCT TCTACAAGCA CTGTATGCAT GTTCTCACTG CTCCCTTACT AGCAAATACA 
ACAGAAGACA AGCCTAGTAA AGATGATTTT CAAACTGCGC AGTTACTAGC ACTTGTATTG 
GAATTGCTAA CATTTTGTGT GGAGCACCAT ACCTACCACA TAAAGAACTA TATTATCAAC 
AAGGACATTC TTCGCAGAGT GCTAGTTCTT ATGGCCTCCA AGCATGCTTT CTTGGCATTA 
TGTGCCCTCC GTTTTAAGAG AAAAATCATT GGATTAAAAG ATGAATTTTA TAACCGCTAC 
ATAATGAAAA GTTTTTTATT TGAACCTGTA GTCAAAGCAT TTCTCAACAA CGGATCTCGT 
TACAATCTGA TGAACTCCGC CATAATAGAA ATGTTTGAAT TTATTAGAGT GGAAGATATA 
AAATCATTAA CTGCCCATGT AATTGAGAAT TATTGGAAAG CACTAGAAGA TGTAGATTAT 
GTACAGACGT TTAAAGGATT AAAATTGAGG TTTGAACAGC AAAGAGAAAG GCAAGACAAC 
CCCAAACTTG ATAGCATGCG GTCTATCTTG AGGAATCATC GATATCGACG AGATGCCAGA 
ACACTAGAAG ACGAAGAAGA GATGTGGTTC AACACAGATG AAGATGACAT GGAAGATGGA 
GAAGCTGTGG TGTCTCCATC TGACAAAACG AAGAATGACG ATGATATTAT GGATCCAATA 
AGTAAATTTA TGGAAAGGAA GAAATTGAAA GAAAGTGAAG AGAAGGAGGT GCTTCTGAAA 
ACTAATCTTT CTGGACGACA GAGCCCAAGT TTCAAACTTT CCTTGTCCAG TGGGACAAAG 
ACTAACCTCA CCAGCCAGTC ATCCGCAACA AGCCTGCCTG GTTCTCCAGG GTCACCTGGC 
TCCCCGGGCT CTCCGGGCTC TCCTGGATCC GTCCCTAAGA GTACATCTCA GACGGCAGCT 
ATTACCACAA AGGGAGGACT CGTGGGTCTG GTAGATTATC CTGATGATGA TGAAGATGAT 
GATGAGGATG AAGACAAGGA AGACACACTA CCAGTGTCAA AGAAAGCAAA ATTTGAGTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997594.1
CDS	833..3295
Translation	MTDTRRRVKVYTLNEDRQWDDRGTGHVSSGYVERLKGMSLLVRAESDGSLLLESKINPNTAYQKQQDTLIVWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVDITQDLVDESEEERFDDMSSPGLELPSCELSRLEEIAELVASSLPSPLRREKLALALENEGYIKKLLELFHVCEDLENIEGLHHLYEIIKGIFLLNRTALFEVMFSEECIMDVIGCLEYDPTLSQPRKHREFLTKTAKFKEVIPISDPELKQKIHQTYRVQYIQDMVLPTPSVFEENMLSTLHSFIFFNKVEIVGMLQEDEKFLTDLFAQLTDEATDEEKRQELVNFLKEFCAFSQTLQPQNRDAFFKTLSNMGILPALEVILGMDDTQVRSAATDIFSYLVEYNPSMVREFVMQEAQQNDDDILLINLIIEHMICDTDPELGGAVQLMGLLRTLVDPENMLATANKTEKTEFLGFFYKHCMHVLTAPLLANTTEDKPSKDDFQTAQLLALVLELLTFCVEHHTYHIKNYIINKDILRRVLVLMASKHAFLALCALRFKRKIIGLKDEFYNRYIMKSFLFEPVVKAFLNNGSRYNLMNSAIIEMFEFIRVEDIKSLTAHVIENYWKALEDVDYVQTFKGLKLRFEQQRERQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDDMEDGEAVVSPSDKTKNDDDIMDPISKFMERKKLKESEEKEVLLKTNLSGRQSPSFKLSLSSGTKTNLTSQSSATSLPGSPGSPGSPGSPGSPGSVPKSTSQTAAITTKGGLVGLVDYPDDDEDDDEDEDKEDTLPVSKKAKFES

Target ORF information:

RefSeq Version	NM_211355.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 4 regulatory subunit 3A (Ppp4r3a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_211355.2

ORF Insert Sequence:

ATGACCGACA CCCGACGGCG GGTGAAGGTC TACACGCTCA ACGAGGACCG GCAGTGGGAC 
GACCGGGGTA CTGGCCATGT GTCCTCCGGC TACGTGGAGA GGCTGAAGGG CATGTCCCTG 
CTCGTCAGGG CGGAGAGCGA CGGTTCTCTT CTTCTAGAGT CCAAAATAAA TCCTAACACT 
GCATACCAGA AACAACAGGA TACTCTGATT GTGTGGTCTG AAGCAGAAAA TTATGACTTG 
GCCCTTAGCT TTCAAGAAAA AGCTGGATGC GATGAAATTT GGGAGAAAAT ATGTCAGGTT 
CAAGGAAAGG ACCCTTCAGT GGACATCACT CAGGACCTGG TAGATGAATC TGAAGAGGAG 
CGATTTGATG ATATGTCCTC ACCAGGTTTA GAATTGCCGT CATGTGAGTT AAGTCGACTT 
GAGGAAATTG CAGAGCTTGT GGCATCATCT TTACCTTCCC CCCTCCGACG TGAGAAACTC 
GCACTAGCAC TGGAAAATGA GGGTTATATT AAAAAGCTTT TAGAGCTTTT TCATGTGTGT 
GAGGATTTGG AAAACATTGA AGGACTGCAC CACTTATATG AAATTATTAA AGGGATCTTC 
CTCTTGAATC GAACTGCTCT TTTTGAAGTT ATGTTCTCTG AGGAATGTAT AATGGACGTC 
ATTGGATGCT TGGAATATGA CCCAACTTTA TCACAACCAC GTAAACACAG GGAATTTTTA 
ACAAAAACAG CCAAGTTTAA AGAAGTAATT CCCATATCAG ATCCTGAGCT GAAACAAAAA 
ATTCATCAGA CATACAGAGT TCAGTATATA CAAGATATGG TTCTCCCTAC CCCTTCAGTC 
TTCGAAGAAA ACATGTTATC AACACTTCAC TCCTTTATCT TTTTCAACAA GGTAGAAATC 
GTTGGTATGT TACAGGAAGA TGAAAAATTT CTGACAGATT TGTTTGCACA ACTAACAGAT 
GAAGCAACAG ATGAGGAAAA AAGACAAGAA TTGGTTAACT TTTTAAAAGA ATTTTGTGCA 
TTTTCTCAAA CGCTACAACC CCAAAACAGA GATGCTTTTT TCAAGACTTT GTCAAATATG 
GGCATATTAC CAGCTTTAGA AGTCATCCTT GGCATGGATG ATACACAGGT GCGAAGTGCT 
GCTACGGATA TATTCTCATA CTTGGTTGAA TATAATCCAT CCATGGTACG AGAGTTTGTC 
ATGCAGGAGG CACAACAGAA TGATGATGAT ATTTTGCTCA TCAACCTCAT TATAGAACAT 
ATGATTTGTG ATACAGATCC CGAGCTTGGA GGGGCAGTCC AACTTATGGG CCTGCTTCGA 
ACTTTAGTTG ACCCAGAGAA CATGCTAGCC ACTGCCAATA AAACAGAAAA GACTGAATTT 
CTGGGTTTCT TCTACAAGCA CTGTATGCAT GTTCTCACTG CTCCCTTACT AGCAAATACA 
ACAGAAGACA AGCCTAGTAA AGATGATTTT CAAACTGCGC AGTTACTAGC ACTTGTATTG 
GAATTGCTAA CATTTTGTGT GGAGCACCAT ACCTACCACA TAAAGAACTA TATTATCAAC 
AAGGACATTC TTCGCAGAGT GCTAGTTCTT ATGGCCTCCA AGCATGCTTT CTTGGCATTA 
TGTGCCCTCC GTTTTAAGAG AAAAATCATT GGATTAAAAG ATGAATTTTA TAACCGCTAC 
ATAATGAAAA GTTTTTTATT TGAACCTGTA GTCAAAGCAT TTCTCAACAA CGGATCTCGT 
TACAATCTGA TGAACTCCGC CATAATAGAA ATGTTTGAAT TTATTAGAGT GGAAGATATA 
AAATCATTAA CTGCCCATGT AATTGAGAAT TATTGGAAAG CACTAGAAGA TGTAGATTAT 
GTACAGACGT TTAAAGGATT AAAATTGAGG TTTGAACAGC AAAGAGAAAG GCAAGACAAC 
CCCAAACTTG ATAGCATGCG GTCTATCTTG AGGAATCATC GATATCGACG AGATGCCAGA 
ACACTAGAAG ACGAAGAAGA GATGTGGTTC AACACAGATG AAGATGACAT GGAAGATGGA 
GAAGCTGTGG TGTCTCCATC TGACAAAACG AAGAATGACG ATGATATTAT GGATCCAATA 
AGTAAATTTA TGGAAAGGAA GAAATTGAAA GAAAGTGAAG AGAAGGAGGT GCTTCTGAAA 
ACTAATCTTT CTGGACGACA GAGCCCAAGT TTCAAACTTT CCTTGTCCAG TGGGACAAAG 
ACTAACCTCA CCAGCCAGTC ATCCGCAACA AGCCTGCCTG GTTCTCCAGG GTCACCTGGC 
TCCCCGGGCT CTCCGGGCTC TCCTGGATCC GTCCCTAAGA GTACATCTCA GACGGCAGCT 
ATTACCACAA AGGGAGGACT CGTGGGTCTG GTAGATTATC CTGATGATGA TGAAGATGAT 
GATGAGGATG AAGACAAGGA AGACACACTA CCAGTGTCAA AGAAAGCAAA ATTTGAGTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppp4r3a ( XM_017315173.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170662.1 Mus musculus protein phosphatase 4 regulatory subunit 3A (Ppp4r3a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu78290
Clone ID Related Accession (Same CDS sequence)	XM_017315173.1
Accession Version	XM_017315173.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein phosphatase 4 regulatory subunit 3A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGACA CCCGACGGCG GGTGAAGGTC TACACGCTCA ACGAGGACCG GCAGTGGGAC 
GACCGGGGTA CTGGCCATGT GTCCTCCGGC TACGTGGAGA GGCTGAAGGG CATGTCCCTG 
CTCGTCAGGG CGGAGAGCGA CGGTTCTCTT CTTCTAGAGT CCAAAATAAA TCCTAACACT 
GCATACCAGA AACAACAGGA TACTCTGATT GTGTGGTCTG AAGCAGAAAA TTATGACTTG 
GCCCTTAGCT TTCAAGAAAA AGCTGGATGC GATGAAATTT GGGAGAAAAT ATGTCAGGTT 
CAAGGAAAGG ACCCTTCAGT GGACATCACT CAGGACCTGG TAGATGAATC TGAAGAGGAG 
CGATTTGATG ATATGTCCTC ACCAGGTTTA GAATTGCCGT CATGTGAGTT AAGTCGACTT 
GAGGAAATTG CAGAGCTTGT GGCATCATCT TTACCTTCCC CCCTCCGACG TGAGAAACTC 
GCACTAGCAC TGGAAAATGA GGGTTATATT AAAAAGCTTT TAGAGCTTTT TCATGTGTGT 
GAGGATTTGG AAAACATTGA AGGACTGCAC CACTTATATG AAATTATTAA AGGGATCTTC 
CTCTTGAATC GAACTGCTCT TTTTGAAGTT ATGTTCTCTG AGGAATGTAT AATGGACGTC 
ATTGGATGCT TGGAATATGA CCCAACTTTA TCACAACCAC GTAAACACAG GGAATTTTTA 
ACAAAAACAG CCAAGTTTAA AGAAGTAATT CCCATATCAG ATCCTGAGCT GAAACAAAAA 
ATTCATCAGA CATACAGAGT TCAGTATATA CAAGATATGG TTCTCCCTAC CCCTTCAGTC 
TTCGAAGAAA ACATGTTATC AACACTTCAC TCCTTTATCT TTTTCAACAA GGTAGAAATC 
GTTGGTATGT TACAGGAAGA TGAAAAATTT CTGACAGATT TGTTTGCACA ACTAACAGAT 
GAAGCAACAG ATGAGGAAAA AAGACAAGAA TTGGTTAACT TTTTAAAAGA ATTTTGTGCA 
TTTTCTCAAA CGCTACAACC CCAAAACAGA GATGCTTTTT TCAAGACTTT GTCAAATATG 
GGCATATTAC CAGCTTTAGA AGTCATCCTT GGCATGGATG ATACACAGGT GCGAAGTGCT 
GCTACGGATA TATTCTCATA CTTGGTTGAA TATAATCCAT CCATGGTACG AGAGTTTGTC 
ATGCAGGAGG CACAACAGAA TGATGATGTA AGTAAGAAGT TAACAGAGCA AAAAATAACC 
AGCAAGGATA TTTTGCTCAT CAACCTCATT ATAGAACATA TGATTTGTGA TACAGATCCC 
GAGCTTGGAG GGGCAGTCCA ACTTATGGGC CTGCTTCGAA CTTTAGTTGA CCCAGAGAAC 
ATGCTAGCCA CTGCCAATAA AACAGAAAAG ACTGAATTTC TGGGTTTCTT CTACAAGCAC 
TGTATGCATG TTCTCACTGC TCCCTTACTA GCAAATACAA CAGAAGACAA GCCTAGTAAA 
GATGATTTTC AAACTGCGCA GTTACTAGCA CTTGTATTGG AATTGCTAAC ATTTTGTGTG 
GAGCACCATA CCTACCACAT AAAGAACTAT ATTATCAACA AGGACATTCT TCGCAGAGTG 
CTAGTTCTTA TGGCCTCCAA GCATGCTTTC TTGGCATTAT GTGCCCTCCG TTTTAAGAGA 
AAAATCATTG GATTAAAAGA TGAATTTTAT AACCGCTACA TAATGAAAAG TTTTTTATTT 
GAACCTGTAG TCAAAGCATT TCTCAACAAC GGATCTCGTT ACAATCTGAT GAACTCCGCC 
ATAATAGAAA TGTTTGAATT TATTAGAGTG GAAGATATAA AATCATTAAC TGCCCATGTA 
ATTGAGAATT ATTGGAAAGC ACTAGAAGAT GTAGATTATG TACAGACGTT TAAAGGATTA 
AAATTGAGGT TTGAACAGCA AAGAGAAAGG CAAGACAACC CCAAACTTGA TAGCATGCGG 
TCTATCTTGA GGAATCATCG ATATCGACGA GATGCCAGAA CACTAGAAGA CGAAGAAGAG 
ATGTGGTTCA ACACAGATGA AGATGACATG GAAGATGGAG AAGCTGTGGT GTCTCCATCT 
GACAAAACGA AGAATGACGA TGATATTATG GATCCAATAA GTAAATTTAT GGAAAGGAAG 
AAATGTATGT TGAAACAAAG AGCAAGGGAA ATGAAAGAAA GTGAAGAGAA GGAGGTGCTT 
CTGAAAACTA ATCTTTCTGG ACGACAGAGC CCAAGTTTCA AACTTTCCTT GTCCAGTGGG 
ACAAAGACTA ACCTCACCAG CCAGTCATCC GCAACAAGCC TGCCTGGTTC TCCAGGGTCA 
CCTGGCTCCC CGGGCTCTCC GGGCTCTCCT GGATCCGTCC CTAAGAGTAC ATCTCAGACG 
GCAGCTATTA CCACAAAGGG AGGACTCGTG GGTCTGGTAG ATTATCCTGA TGATGATGAA 
GATGATGATG AGGATGAAGA CAAGGAAGAC ACACTACCAG TGTCAAAGAA AGCAAAATTT 
GAGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170662.1
CDS	828..3356
Translation	MTDTRRRVKVYTLNEDRQWDDRGTGHVSSGYVERLKGMSLLVRAESDGSLLLESKINPNTAYQKQQDTLIVWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVDITQDLVDESEEERFDDMSSPGLELPSCELSRLEEIAELVASSLPSPLRREKLALALENEGYIKKLLELFHVCEDLENIEGLHHLYEIIKGIFLLNRTALFEVMFSEECIMDVIGCLEYDPTLSQPRKHREFLTKTAKFKEVIPISDPELKQKIHQTYRVQYIQDMVLPTPSVFEENMLSTLHSFIFFNKVEIVGMLQEDEKFLTDLFAQLTDEATDEEKRQELVNFLKEFCAFSQTLQPQNRDAFFKTLSNMGILPALEVILGMDDTQVRSAATDIFSYLVEYNPSMVREFVMQEAQQNDDVSKKLTEQKITSKDILLINLIIEHMICDTDPELGGAVQLMGLLRTLVDPENMLATANKTEKTEFLGFFYKHCMHVLTAPLLANTTEDKPSKDDFQTAQLLALVLELLTFCVEHHTYHIKNYIINKDILRRVLVLMASKHAFLALCALRFKRKIIGLKDEFYNRYIMKSFLFEPVVKAFLNNGSRYNLMNSAIIEMFEFIRVEDIKSLTAHVIENYWKALEDVDYVQTFKGLKLRFEQQRERQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDDMEDGEAVVSPSDKTKNDDDIMDPISKFMERKKCMLKQRAREMKESEEKEVLLKTNLSGRQSPSFKLSLSSGTKTNLTSQSSATSLPGSPGSPGSPGSPGSPGSVPKSTSQTAAITTKGGLVGLVDYPDDDEDDDEDEDKEDTLPVSKKAKFES

Target ORF information:

RefSeq Version	XM_017315173.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 4 regulatory subunit 3A (Ppp4r3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315173.1

ORF Insert Sequence:

ATGACCGACA CCCGACGGCG GGTGAAGGTC TACACGCTCA ACGAGGACCG GCAGTGGGAC 
GACCGGGGTA CTGGCCATGT GTCCTCCGGC TACGTGGAGA GGCTGAAGGG CATGTCCCTG 
CTCGTCAGGG CGGAGAGCGA CGGTTCTCTT CTTCTAGAGT CCAAAATAAA TCCTAACACT 
GCATACCAGA AACAACAGGA TACTCTGATT GTGTGGTCTG AAGCAGAAAA TTATGACTTG 
GCCCTTAGCT TTCAAGAAAA AGCTGGATGC GATGAAATTT GGGAGAAAAT ATGTCAGGTT 
CAAGGAAAGG ACCCTTCAGT GGACATCACT CAGGACCTGG TAGATGAATC TGAAGAGGAG 
CGATTTGATG ATATGTCCTC ACCAGGTTTA GAATTGCCGT CATGTGAGTT AAGTCGACTT 
GAGGAAATTG CAGAGCTTGT GGCATCATCT TTACCTTCCC CCCTCCGACG TGAGAAACTC 
GCACTAGCAC TGGAAAATGA GGGTTATATT AAAAAGCTTT TAGAGCTTTT TCATGTGTGT 
GAGGATTTGG AAAACATTGA AGGACTGCAC CACTTATATG AAATTATTAA AGGGATCTTC 
CTCTTGAATC GAACTGCTCT TTTTGAAGTT ATGTTCTCTG AGGAATGTAT AATGGACGTC 
ATTGGATGCT TGGAATATGA CCCAACTTTA TCACAACCAC GTAAACACAG GGAATTTTTA 
ACAAAAACAG CCAAGTTTAA AGAAGTAATT CCCATATCAG ATCCTGAGCT GAAACAAAAA 
ATTCATCAGA CATACAGAGT TCAGTATATA CAAGATATGG TTCTCCCTAC CCCTTCAGTC 
TTCGAAGAAA ACATGTTATC AACACTTCAC TCCTTTATCT TTTTCAACAA GGTAGAAATC 
GTTGGTATGT TACAGGAAGA TGAAAAATTT CTGACAGATT TGTTTGCACA ACTAACAGAT 
GAAGCAACAG ATGAGGAAAA AAGACAAGAA TTGGTTAACT TTTTAAAAGA ATTTTGTGCA 
TTTTCTCAAA CGCTACAACC CCAAAACAGA GATGCTTTTT TCAAGACTTT GTCAAATATG 
GGCATATTAC CAGCTTTAGA AGTCATCCTT GGCATGGATG ATACACAGGT GCGAAGTGCT 
GCTACGGATA TATTCTCATA CTTGGTTGAA TATAATCCAT CCATGGTACG AGAGTTTGTC 
ATGCAGGAGG CACAACAGAA TGATGATGTA AGTAAGAAGT TAACAGAGCA AAAAATAACC 
AGCAAGGATA TTTTGCTCAT CAACCTCATT ATAGAACATA TGATTTGTGA TACAGATCCC 
GAGCTTGGAG GGGCAGTCCA ACTTATGGGC CTGCTTCGAA CTTTAGTTGA CCCAGAGAAC 
ATGCTAGCCA CTGCCAATAA AACAGAAAAG ACTGAATTTC TGGGTTTCTT CTACAAGCAC 
TGTATGCATG TTCTCACTGC TCCCTTACTA GCAAATACAA CAGAAGACAA GCCTAGTAAA 
GATGATTTTC AAACTGCGCA GTTACTAGCA CTTGTATTGG AATTGCTAAC ATTTTGTGTG 
GAGCACCATA CCTACCACAT AAAGAACTAT ATTATCAACA AGGACATTCT TCGCAGAGTG 
CTAGTTCTTA TGGCCTCCAA GCATGCTTTC TTGGCATTAT GTGCCCTCCG TTTTAAGAGA 
AAAATCATTG GATTAAAAGA TGAATTTTAT AACCGCTACA TAATGAAAAG TTTTTTATTT 
GAACCTGTAG TCAAAGCATT TCTCAACAAC GGATCTCGTT ACAATCTGAT GAACTCCGCC 
ATAATAGAAA TGTTTGAATT TATTAGAGTG GAAGATATAA AATCATTAAC TGCCCATGTA 
ATTGAGAATT ATTGGAAAGC ACTAGAAGAT GTAGATTATG TACAGACGTT TAAAGGATTA 
AAATTGAGGT TTGAACAGCA AAGAGAAAGG CAAGACAACC CCAAACTTGA TAGCATGCGG 
TCTATCTTGA GGAATCATCG ATATCGACGA GATGCCAGAA CACTAGAAGA CGAAGAAGAG 
ATGTGGTTCA ACACAGATGA AGATGACATG GAAGATGGAG AAGCTGTGGT GTCTCCATCT 
GACAAAACGA AGAATGACGA TGATATTATG GATCCAATAA GTAAATTTAT GGAAAGGAAG 
AAATGTATGT TGAAACAAAG AGCAAGGGAA ATGAAAGAAA GTGAAGAGAA GGAGGTGCTT 
CTGAAAACTA ATCTTTCTGG ACGACAGAGC CCAAGTTTCA AACTTTCCTT GTCCAGTGGG 
ACAAAGACTA ACCTCACCAG CCAGTCATCC GCAACAAGCC TGCCTGGTTC TCCAGGGTCA 
CCTGGCTCCC CGGGCTCTCC GGGCTCTCCT GGATCCGTCC CTAAGAGTAC ATCTCAGACG 
GCAGCTATTA CCACAAAGGG AGGACTCGTG GGTCTGGTAG ATTATCCTGA TGATGATGAA 
GATGATGATG AGGATGAAGA CAAGGAAGAC ACACTACCAG TGTCAAAGAA AGCAAAATTT 
GAGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppp4r3a ( XM_017315174.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170663.1 Mus musculus protein phosphatase 4 regulatory subunit 3A (Ppp4r3a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu78291
Clone ID Related Accession (Same CDS sequence)	XM_017315174.2 , XM_017315174.1
Accession Version	XM_017315174.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein phosphatase 4 regulatory subunit 3A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315174.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGACA CCCGACGGCG GGTGAAGGTC TACACGCTCA ACGAGGACCG GCAGTGGGAC 
GACCGGGGTA CTGGCCATGT GTCCTCCGGC TACGTGGAGA GGCTGAAGGG CATGTCCCTG 
CTCGTCAGGG CGGAGAGCGA CGGTTCTCTT CTTCTAGAGT CCAAAATAAA TCCTAACACT 
GCATACCAGA AACAACAGGA TACTCTGATT GTGTGGTCTG AAGCAGAAAA TTATGACTTG 
GCCCTTAGCT TTCAAGAAAA AGCTGGATGC GATGAAATTT GGGAGAAAAT ATGTCAGGTT 
CAAGGAAAGG ACCCTTCAGT GGACATCACT CAGGACCTGG TAGATGAATC TGAAGAGGAG 
CGATTTGATG ATATGTCCTC ACCAGGTTTA GAATTGCCGT CATGTGAGTT AAGTCGACTT 
GAGGAAATTG CAGAGCTTGT GGCATCATCT TTACCTTCCC CCCTCCGACG TGAGAAACTC 
GCACTAGCAC TGGAAAATGA GGGTTATATT AAAAAGCTTT TAGAGCTTTT TCATGTGTGT 
GAGGATTTGG AAAACATTGA AGGACTGCAC CACTTATATG AAATTATTAA AGGGATCTTC 
CTCTTGAATC GAACTGCTCT TTTTGAAGTT ATGTTCTCTG AGGAATGTAT AATGGACGTC 
ATTGGATGCT TGGAATATGA CCCAACTTTA TCACAACCAC GTAAACACAG GGAATTTTTA 
ACAAAAACAG CCAAGTTTAA AGAAGTAATT CCCATATCAG ATCCTGAGCT GAAACAAAAA 
ATTCATCAGA CATACAGAGT TCAGTATATA CAAGATATGG TTCTCCCTAC CCCTTCAGTC 
TTCGAAGAAA ACATGTTATC AACACTTCAC TCCTTTATCT TTTTCAACAA GGTAGAAATC 
GTTGGTATGT TACAGGAAGA TGAAAAATTT CTGACAGATT TGTTTGCACA ACTAACAGAT 
GAAGCAACAG ATGAGGAAAA AAGACAAGAA TTGGTTAACT TTTTAAAAGA ATTTTGTGCA 
TTTTCTCAAA CGCTACAACC CCAAAACAGA GATGCTTTTT TCAAGACTTT GTCAAATATG 
GGCATATTAC CAGCTTTAGA AGTCATCCTT GGCATGGATG ATACACAGGT GCGAAGTGCT 
GCTACGGATA TATTCTCATA CTTGGTTGAA TATAATCCAT CCATGGTACG AGAGTTTGTC 
ATGCAGGAGG CACAACAGAA TGATGATGAT ATTTTGCTCA TCAACCTCAT TATAGAACAT 
ATGATTTGTG ATACAGATCC CGAGCTTGGA GGGGCAGTCC AACTTATGGG CCTGCTTCGA 
ACTTTAGTTG ACCCAGAGAA CATGCTAGCC ACTGCCAATA AAACAGAAAA GACTGAATTT 
CTGGGTTTCT TCTACAAGCA CTGTATGCAT GTTCTCACTG CTCCCTTACT AGCAAATACA 
ACAGAAGACA AGCCTAGTAA AGATGATTTT CAAACTGCGC AGTTACTAGC ACTTGTATTG 
GAATTGCTAA CATTTTGTGT GGAGCACCAT ACCTACCACA TAAAGAACTA TATTATCAAC 
AAGGACATTC TTCGCAGAGT GCTAGTTCTT ATGGCCTCCA AGCATGCTTT CTTGGCATTA 
TGTGCCCTCC GTTTTAAGAG AAAAATCATT GGATTAAAAG ATGAATTTTA TAACCGCTAC 
ATAATGAAAA GTTTTTTATT TGAACCTGTA GTCAAAGCAT TTCTCAACAA CGGATCTCGT 
TACAATCTGA TGAACTCCGC CATAATAGAA ATGTTTGAAT TTATTAGAGT GGAAGATATA 
AAATCATTAA CTGCCCATGT AATTGAGAAT TATTGGAAAG CACTAGAAGA TGTAGATTAT 
GTACAGACGT TTAAAGGATT AAAATTGAGG TTTGAACAGC AAAGAGAAAG GCAAGACAAC 
CCCAAACTTG ATAGCATGCG GTCTATCTTG AGGAATCATC GATATCGACG AGATGCCAGA 
ACACTAGAAG ACGAAGAAGA GATGTGGTTC AACACAGATG AAGATGACAT GGAAGATGGA 
GAAGCTGTGG TGTCTCCATC TGACAAAACG AAGAATGACG ATGATATTAT GGATCCAATA 
AGTAAATTTA TGGAAAGGAA GAAATGTATG TTGAAACAAA GAGCAAGGGA AATGAAAGAA 
AGTGAAGAGA AGGAGGTGCT TCTGAAAACT AATCTTTCTG GACGACAGAG CCCAAGTTTC 
AAACTTTCCT TGTCCAGTGG GACAAAGACT AACCTCACCA GCCAGTCATC CGCAACAAGC 
CTGCCTGGTT CTCCAGGGTC ACCTGGCTCC CCGGGCTCTC CGGGCTCTCC TGGATCCGTC 
CCTAAGAGTA CATCTCAGAC GGCAGCTATT ACCACAAAGG GAGGACTCGT GGGTCTGGTA 
GATTATCCTG ATGATGATGA AGATGATGAT GAGGATGAAG ACAAGGAAGA CACACTACCA 
GTGTCAAAGA AAGCAAAATT TGAGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170663.1
CDS	757..3246
Translation	MTDTRRRVKVYTLNEDRQWDDRGTGHVSSGYVERLKGMSLLVRAESDGSLLLESKINPNTAYQKQQDTLIVWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVDITQDLVDESEEERFDDMSSPGLELPSCELSRLEEIAELVASSLPSPLRREKLALALENEGYIKKLLELFHVCEDLENIEGLHHLYEIIKGIFLLNRTALFEVMFSEECIMDVIGCLEYDPTLSQPRKHREFLTKTAKFKEVIPISDPELKQKIHQTYRVQYIQDMVLPTPSVFEENMLSTLHSFIFFNKVEIVGMLQEDEKFLTDLFAQLTDEATDEEKRQELVNFLKEFCAFSQTLQPQNRDAFFKTLSNMGILPALEVILGMDDTQVRSAATDIFSYLVEYNPSMVREFVMQEAQQNDDDILLINLIIEHMICDTDPELGGAVQLMGLLRTLVDPENMLATANKTEKTEFLGFFYKHCMHVLTAPLLANTTEDKPSKDDFQTAQLLALVLELLTFCVEHHTYHIKNYIINKDILRRVLVLMASKHAFLALCALRFKRKIIGLKDEFYNRYIMKSFLFEPVVKAFLNNGSRYNLMNSAIIEMFEFIRVEDIKSLTAHVIENYWKALEDVDYVQTFKGLKLRFEQQRERQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDDMEDGEAVVSPSDKTKNDDDIMDPISKFMERKKCMLKQRAREMKESEEKEVLLKTNLSGRQSPSFKLSLSSGTKTNLTSQSSATSLPGSPGSPGSPGSPGSPGSVPKSTSQTAAITTKGGLVGLVDYPDDDEDDDEDEDKEDTLPVSKKAKFES

Target ORF information:

RefSeq Version	XM_017315174.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 4 regulatory subunit 3A (Ppp4r3a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315174.2

ORF Insert Sequence:

ATGACCGACA CCCGACGGCG GGTGAAGGTC TACACGCTCA ACGAGGACCG GCAGTGGGAC 
GACCGGGGTA CTGGCCATGT GTCCTCCGGC TACGTGGAGA GGCTGAAGGG CATGTCCCTG 
CTCGTCAGGG CGGAGAGCGA CGGTTCTCTT CTTCTAGAGT CCAAAATAAA TCCTAACACT 
GCATACCAGA AACAACAGGA TACTCTGATT GTGTGGTCTG AAGCAGAAAA TTATGACTTG 
GCCCTTAGCT TTCAAGAAAA AGCTGGATGC GATGAAATTT GGGAGAAAAT ATGTCAGGTT 
CAAGGAAAGG ACCCTTCAGT GGACATCACT CAGGACCTGG TAGATGAATC TGAAGAGGAG 
CGATTTGATG ATATGTCCTC ACCAGGTTTA GAATTGCCGT CATGTGAGTT AAGTCGACTT 
GAGGAAATTG CAGAGCTTGT GGCATCATCT TTACCTTCCC CCCTCCGACG TGAGAAACTC 
GCACTAGCAC TGGAAAATGA GGGTTATATT AAAAAGCTTT TAGAGCTTTT TCATGTGTGT 
GAGGATTTGG AAAACATTGA AGGACTGCAC CACTTATATG AAATTATTAA AGGGATCTTC 
CTCTTGAATC GAACTGCTCT TTTTGAAGTT ATGTTCTCTG AGGAATGTAT AATGGACGTC 
ATTGGATGCT TGGAATATGA CCCAACTTTA TCACAACCAC GTAAACACAG GGAATTTTTA 
ACAAAAACAG CCAAGTTTAA AGAAGTAATT CCCATATCAG ATCCTGAGCT GAAACAAAAA 
ATTCATCAGA CATACAGAGT TCAGTATATA CAAGATATGG TTCTCCCTAC CCCTTCAGTC 
TTCGAAGAAA ACATGTTATC AACACTTCAC TCCTTTATCT TTTTCAACAA GGTAGAAATC 
GTTGGTATGT TACAGGAAGA TGAAAAATTT CTGACAGATT TGTTTGCACA ACTAACAGAT 
GAAGCAACAG ATGAGGAAAA AAGACAAGAA TTGGTTAACT TTTTAAAAGA ATTTTGTGCA 
TTTTCTCAAA CGCTACAACC CCAAAACAGA GATGCTTTTT TCAAGACTTT GTCAAATATG 
GGCATATTAC CAGCTTTAGA AGTCATCCTT GGCATGGATG ATACACAGGT GCGAAGTGCT 
GCTACGGATA TATTCTCATA CTTGGTTGAA TATAATCCAT CCATGGTACG AGAGTTTGTC 
ATGCAGGAGG CACAACAGAA TGATGATGAT ATTTTGCTCA TCAACCTCAT TATAGAACAT 
ATGATTTGTG ATACAGATCC CGAGCTTGGA GGGGCAGTCC AACTTATGGG CCTGCTTCGA 
ACTTTAGTTG ACCCAGAGAA CATGCTAGCC ACTGCCAATA AAACAGAAAA GACTGAATTT 
CTGGGTTTCT TCTACAAGCA CTGTATGCAT GTTCTCACTG CTCCCTTACT AGCAAATACA 
ACAGAAGACA AGCCTAGTAA AGATGATTTT CAAACTGCGC AGTTACTAGC ACTTGTATTG 
GAATTGCTAA CATTTTGTGT GGAGCACCAT ACCTACCACA TAAAGAACTA TATTATCAAC 
AAGGACATTC TTCGCAGAGT GCTAGTTCTT ATGGCCTCCA AGCATGCTTT CTTGGCATTA 
TGTGCCCTCC GTTTTAAGAG AAAAATCATT GGATTAAAAG ATGAATTTTA TAACCGCTAC 
ATAATGAAAA GTTTTTTATT TGAACCTGTA GTCAAAGCAT TTCTCAACAA CGGATCTCGT 
TACAATCTGA TGAACTCCGC CATAATAGAA ATGTTTGAAT TTATTAGAGT GGAAGATATA 
AAATCATTAA CTGCCCATGT AATTGAGAAT TATTGGAAAG CACTAGAAGA TGTAGATTAT 
GTACAGACGT TTAAAGGATT AAAATTGAGG TTTGAACAGC AAAGAGAAAG GCAAGACAAC 
CCCAAACTTG ATAGCATGCG GTCTATCTTG AGGAATCATC GATATCGACG AGATGCCAGA 
ACACTAGAAG ACGAAGAAGA GATGTGGTTC AACACAGATG AAGATGACAT GGAAGATGGA 
GAAGCTGTGG TGTCTCCATC TGACAAAACG AAGAATGACG ATGATATTAT GGATCCAATA 
AGTAAATTTA TGGAAAGGAA GAAATGTATG TTGAAACAAA GAGCAAGGGA AATGAAAGAA 
AGTGAAGAGA AGGAGGTGCT TCTGAAAACT AATCTTTCTG GACGACAGAG CCCAAGTTTC 
AAACTTTCCT TGTCCAGTGG GACAAAGACT AACCTCACCA GCCAGTCATC CGCAACAAGC 
CTGCCTGGTT CTCCAGGGTC ACCTGGCTCC CCGGGCTCTC CGGGCTCTCC TGGATCCGTC 
CCTAAGAGTA CATCTCAGAC GGCAGCTATT ACCACAAAGG GAGGACTCGT GGGTCTGGTA 
GATTATCCTG ATGATGATGA AGATGATGAT GAGGATGAAG ACAAGGAAGA CACACTACCA 
GTGTCAAAGA AAGCAAAATT TGAGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppp4r3a ( XM_017315174.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170663.1 Mus musculus SMEK homolog 1, suppressor of mek1 (Dictyostelium) (Smek1), transcript variant X2, mRNA.

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CloneID	OMu78291
Clone ID Related Accession (Same CDS sequence)	XM_017315174.2 , XM_017315174.1
Accession Version	XM_017315174.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein phosphatase 4 regulatory subunit 3A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGACA CCCGACGGCG GGTGAAGGTC TACACGCTCA ACGAGGACCG GCAGTGGGAC 
GACCGGGGTA CTGGCCATGT GTCCTCCGGC TACGTGGAGA GGCTGAAGGG CATGTCCCTG 
CTCGTCAGGG CGGAGAGCGA CGGTTCTCTT CTTCTAGAGT CCAAAATAAA TCCTAACACT 
GCATACCAGA AACAACAGGA TACTCTGATT GTGTGGTCTG AAGCAGAAAA TTATGACTTG 
GCCCTTAGCT TTCAAGAAAA AGCTGGATGC GATGAAATTT GGGAGAAAAT ATGTCAGGTT 
CAAGGAAAGG ACCCTTCAGT GGACATCACT CAGGACCTGG TAGATGAATC TGAAGAGGAG 
CGATTTGATG ATATGTCCTC ACCAGGTTTA GAATTGCCGT CATGTGAGTT AAGTCGACTT 
GAGGAAATTG CAGAGCTTGT GGCATCATCT TTACCTTCCC CCCTCCGACG TGAGAAACTC 
GCACTAGCAC TGGAAAATGA GGGTTATATT AAAAAGCTTT TAGAGCTTTT TCATGTGTGT 
GAGGATTTGG AAAACATTGA AGGACTGCAC CACTTATATG AAATTATTAA AGGGATCTTC 
CTCTTGAATC GAACTGCTCT TTTTGAAGTT ATGTTCTCTG AGGAATGTAT AATGGACGTC 
ATTGGATGCT TGGAATATGA CCCAACTTTA TCACAACCAC GTAAACACAG GGAATTTTTA 
ACAAAAACAG CCAAGTTTAA AGAAGTAATT CCCATATCAG ATCCTGAGCT GAAACAAAAA 
ATTCATCAGA CATACAGAGT TCAGTATATA CAAGATATGG TTCTCCCTAC CCCTTCAGTC 
TTCGAAGAAA ACATGTTATC AACACTTCAC TCCTTTATCT TTTTCAACAA GGTAGAAATC 
GTTGGTATGT TACAGGAAGA TGAAAAATTT CTGACAGATT TGTTTGCACA ACTAACAGAT 
GAAGCAACAG ATGAGGAAAA AAGACAAGAA TTGGTTAACT TTTTAAAAGA ATTTTGTGCA 
TTTTCTCAAA CGCTACAACC CCAAAACAGA GATGCTTTTT TCAAGACTTT GTCAAATATG 
GGCATATTAC CAGCTTTAGA AGTCATCCTT GGCATGGATG ATACACAGGT GCGAAGTGCT 
GCTACGGATA TATTCTCATA CTTGGTTGAA TATAATCCAT CCATGGTACG AGAGTTTGTC 
ATGCAGGAGG CACAACAGAA TGATGATGAT ATTTTGCTCA TCAACCTCAT TATAGAACAT 
ATGATTTGTG ATACAGATCC CGAGCTTGGA GGGGCAGTCC AACTTATGGG CCTGCTTCGA 
ACTTTAGTTG ACCCAGAGAA CATGCTAGCC ACTGCCAATA AAACAGAAAA GACTGAATTT 
CTGGGTTTCT TCTACAAGCA CTGTATGCAT GTTCTCACTG CTCCCTTACT AGCAAATACA 
ACAGAAGACA AGCCTAGTAA AGATGATTTT CAAACTGCGC AGTTACTAGC ACTTGTATTG 
GAATTGCTAA CATTTTGTGT GGAGCACCAT ACCTACCACA TAAAGAACTA TATTATCAAC 
AAGGACATTC TTCGCAGAGT GCTAGTTCTT ATGGCCTCCA AGCATGCTTT CTTGGCATTA 
TGTGCCCTCC GTTTTAAGAG AAAAATCATT GGATTAAAAG ATGAATTTTA TAACCGCTAC 
ATAATGAAAA GTTTTTTATT TGAACCTGTA GTCAAAGCAT TTCTCAACAA CGGATCTCGT 
TACAATCTGA TGAACTCCGC CATAATAGAA ATGTTTGAAT TTATTAGAGT GGAAGATATA 
AAATCATTAA CTGCCCATGT AATTGAGAAT TATTGGAAAG CACTAGAAGA TGTAGATTAT 
GTACAGACGT TTAAAGGATT AAAATTGAGG TTTGAACAGC AAAGAGAAAG GCAAGACAAC 
CCCAAACTTG ATAGCATGCG GTCTATCTTG AGGAATCATC GATATCGACG AGATGCCAGA 
ACACTAGAAG ACGAAGAAGA GATGTGGTTC AACACAGATG AAGATGACAT GGAAGATGGA 
GAAGCTGTGG TGTCTCCATC TGACAAAACG AAGAATGACG ATGATATTAT GGATCCAATA 
AGTAAATTTA TGGAAAGGAA GAAATGTATG TTGAAACAAA GAGCAAGGGA AATGAAAGAA 
AGTGAAGAGA AGGAGGTGCT TCTGAAAACT AATCTTTCTG GACGACAGAG CCCAAGTTTC 
AAACTTTCCT TGTCCAGTGG GACAAAGACT AACCTCACCA GCCAGTCATC CGCAACAAGC 
CTGCCTGGTT CTCCAGGGTC ACCTGGCTCC CCGGGCTCTC CGGGCTCTCC TGGATCCGTC 
CCTAAGAGTA CATCTCAGAC GGCAGCTATT ACCACAAAGG GAGGACTCGT GGGTCTGGTA 
GATTATCCTG ATGATGATGA AGATGATGAT GAGGATGAAG ACAAGGAAGA CACACTACCA 
GTGTCAAAGA AAGCAAAATT TGAGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170663.1
CDS	757..3246
Translation	MTDTRRRVKVYTLNEDRQWDDRGTGHVSSGYVERLKGMSLLVRAESDGSLLLESKINPNTAYQKQQDTLIVWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVDITQDLVDESEEERFDDMSSPGLELPSCELSRLEEIAELVASSLPSPLRREKLALALENEGYIKKLLELFHVCEDLENIEGLHHLYEIIKGIFLLNRTALFEVMFSEECIMDVIGCLEYDPTLSQPRKHREFLTKTAKFKEVIPISDPELKQKIHQTYRVQYIQDMVLPTPSVFEENMLSTLHSFIFFNKVEIVGMLQEDEKFLTDLFAQLTDEATDEEKRQELVNFLKEFCAFSQTLQPQNRDAFFKTLSNMGILPALEVILGMDDTQVRSAATDIFSYLVEYNPSMVREFVMQEAQQNDDDILLINLIIEHMICDTDPELGGAVQLMGLLRTLVDPENMLATANKTEKTEFLGFFYKHCMHVLTAPLLANTTEDKPSKDDFQTAQLLALVLELLTFCVEHHTYHIKNYIINKDILRRVLVLMASKHAFLALCALRFKRKIIGLKDEFYNRYIMKSFLFEPVVKAFLNNGSRYNLMNSAIIEMFEFIRVEDIKSLTAHVIENYWKALEDVDYVQTFKGLKLRFEQQRERQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDDMEDGEAVVSPSDKTKNDDDIMDPISKFMERKKCMLKQRAREMKESEEKEVLLKTNLSGRQSPSFKLSLSSGTKTNLTSQSSATSLPGSPGSPGSPGSPGSPGSVPKSTSQTAAITTKGGLVGLVDYPDDDEDDDEDEDKEDTLPVSKKAKFES

Target ORF information:

RefSeq Version	XM_017315174.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SMEK homolog 1, suppressor of mek1 (Dictyostelium) (Smek1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315174.1

ORF Insert Sequence:

ATGACCGACA CCCGACGGCG GGTGAAGGTC TACACGCTCA ACGAGGACCG GCAGTGGGAC 
GACCGGGGTA CTGGCCATGT GTCCTCCGGC TACGTGGAGA GGCTGAAGGG CATGTCCCTG 
CTCGTCAGGG CGGAGAGCGA CGGTTCTCTT CTTCTAGAGT CCAAAATAAA TCCTAACACT 
GCATACCAGA AACAACAGGA TACTCTGATT GTGTGGTCTG AAGCAGAAAA TTATGACTTG 
GCCCTTAGCT TTCAAGAAAA AGCTGGATGC GATGAAATTT GGGAGAAAAT ATGTCAGGTT 
CAAGGAAAGG ACCCTTCAGT GGACATCACT CAGGACCTGG TAGATGAATC TGAAGAGGAG 
CGATTTGATG ATATGTCCTC ACCAGGTTTA GAATTGCCGT CATGTGAGTT AAGTCGACTT 
GAGGAAATTG CAGAGCTTGT GGCATCATCT TTACCTTCCC CCCTCCGACG TGAGAAACTC 
GCACTAGCAC TGGAAAATGA GGGTTATATT AAAAAGCTTT TAGAGCTTTT TCATGTGTGT 
GAGGATTTGG AAAACATTGA AGGACTGCAC CACTTATATG AAATTATTAA AGGGATCTTC 
CTCTTGAATC GAACTGCTCT TTTTGAAGTT ATGTTCTCTG AGGAATGTAT AATGGACGTC 
ATTGGATGCT TGGAATATGA CCCAACTTTA TCACAACCAC GTAAACACAG GGAATTTTTA 
ACAAAAACAG CCAAGTTTAA AGAAGTAATT CCCATATCAG ATCCTGAGCT GAAACAAAAA 
ATTCATCAGA CATACAGAGT TCAGTATATA CAAGATATGG TTCTCCCTAC CCCTTCAGTC 
TTCGAAGAAA ACATGTTATC AACACTTCAC TCCTTTATCT TTTTCAACAA GGTAGAAATC 
GTTGGTATGT TACAGGAAGA TGAAAAATTT CTGACAGATT TGTTTGCACA ACTAACAGAT 
GAAGCAACAG ATGAGGAAAA AAGACAAGAA TTGGTTAACT TTTTAAAAGA ATTTTGTGCA 
TTTTCTCAAA CGCTACAACC CCAAAACAGA GATGCTTTTT TCAAGACTTT GTCAAATATG 
GGCATATTAC CAGCTTTAGA AGTCATCCTT GGCATGGATG ATACACAGGT GCGAAGTGCT 
GCTACGGATA TATTCTCATA CTTGGTTGAA TATAATCCAT CCATGGTACG AGAGTTTGTC 
ATGCAGGAGG CACAACAGAA TGATGATGAT ATTTTGCTCA TCAACCTCAT TATAGAACAT 
ATGATTTGTG ATACAGATCC CGAGCTTGGA GGGGCAGTCC AACTTATGGG CCTGCTTCGA 
ACTTTAGTTG ACCCAGAGAA CATGCTAGCC ACTGCCAATA AAACAGAAAA GACTGAATTT 
CTGGGTTTCT TCTACAAGCA CTGTATGCAT GTTCTCACTG CTCCCTTACT AGCAAATACA 
ACAGAAGACA AGCCTAGTAA AGATGATTTT CAAACTGCGC AGTTACTAGC ACTTGTATTG 
GAATTGCTAA CATTTTGTGT GGAGCACCAT ACCTACCACA TAAAGAACTA TATTATCAAC 
AAGGACATTC TTCGCAGAGT GCTAGTTCTT ATGGCCTCCA AGCATGCTTT CTTGGCATTA 
TGTGCCCTCC GTTTTAAGAG AAAAATCATT GGATTAAAAG ATGAATTTTA TAACCGCTAC 
ATAATGAAAA GTTTTTTATT TGAACCTGTA GTCAAAGCAT TTCTCAACAA CGGATCTCGT 
TACAATCTGA TGAACTCCGC CATAATAGAA ATGTTTGAAT TTATTAGAGT GGAAGATATA 
AAATCATTAA CTGCCCATGT AATTGAGAAT TATTGGAAAG CACTAGAAGA TGTAGATTAT 
GTACAGACGT TTAAAGGATT AAAATTGAGG TTTGAACAGC AAAGAGAAAG GCAAGACAAC 
CCCAAACTTG ATAGCATGCG GTCTATCTTG AGGAATCATC GATATCGACG AGATGCCAGA 
ACACTAGAAG ACGAAGAAGA GATGTGGTTC AACACAGATG AAGATGACAT GGAAGATGGA 
GAAGCTGTGG TGTCTCCATC TGACAAAACG AAGAATGACG ATGATATTAT GGATCCAATA 
AGTAAATTTA TGGAAAGGAA GAAATGTATG TTGAAACAAA GAGCAAGGGA AATGAAAGAA 
AGTGAAGAGA AGGAGGTGCT TCTGAAAACT AATCTTTCTG GACGACAGAG CCCAAGTTTC 
AAACTTTCCT TGTCCAGTGG GACAAAGACT AACCTCACCA GCCAGTCATC CGCAACAAGC 
CTGCCTGGTT CTCCAGGGTC ACCTGGCTCC CCGGGCTCTC CGGGCTCTCC TGGATCCGTC 
CCTAAGAGTA CATCTCAGAC GGCAGCTATT ACCACAAAGG GAGGACTCGT GGGTCTGGTA 
GATTATCCTG ATGATGATGA AGATGATGAT GAGGATGAAG ACAAGGAAGA CACACTACCA 
GTGTCAAAGA AAGCAAAATT TGAGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Smek1/2 is a nuclear chaperone and cofactor for cleaved Wnt receptor Ryk, regulating cortical neurogenesis.
Proceedings of the National Academy of Sciences of the United States of America114(50)E10717-E10725(2017 Dec)
Chang WH,Choi SH,Moon BS,Cai M,Lyu J,Bai J,Gao F,Hajjali I,Zhao Z,Campbell DB,Weiner LP,Lu W

Smek promotes corticogenesis through regulating Mbd3's stability and Mbd3/NuRD complex recruitment to genes associated with neurogenesis.
PLoS biology15(5)e2001220(2017 May)
Moon BS,Yun HM,Chang WH,Steele BH,Cai M,Choi SH,Lu W

Protein phosphatase 4 and Smek complex negatively regulate Par3 and promote neuronal differentiation of neural stem/progenitor cells.
Cell reports5(3)593-600(2013 Nov)
Lyu J,Kim HR,Yamamoto V,Choi SH,Wei Z,Joo CK,Lu W

Smek promotes histone deacetylation to suppress transcription of Wnt target gene brachyury in pluripotent embryonic stem cells.
Cell research21(6)911-21(2011 Jun)
Lyu J,Jho EH,Lu W

Suppressor of MEK null (SMEK)/protein phosphatase 4 catalytic subunit (PP4C) is a key regulator of hepatic gluconeogenesis.
Proceedings of the National Academy of Sciences of the United States of America107(41)17704-9(2010 Oct)
Yoon YS,Lee MW,Ryu D,Kim JH,Ma H,Seo WY,Kim YN,Kim SS,Lee CH,Hunter T,Choi CS,Montminy MR,Koo SH

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Ppp4r3a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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