DPPA4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DPPA4
Entrez Gene ID	55211
Full Name	developmental pluripotency associated 4
Synonyms	2410091M23Rik
General protein information	Preferred Names developmental pluripotency associated 4 Names developmental pluripotency-associated protein 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q13.13
Summary	This gene encodes a nuclear factor that is involved in the maintenance of pluripotency in stem cells and essential for embryogenesis. The encoded protein has a scaffold-attachment factor A/B, acinus and PIAS (SAP) domain that binds DNA and is thought to modify chromatin. Mice with a homozygous knockout of the orthologous gene die during late embryonic development or within hours after birth. Knockout embryos are normal in size at embryonic day 18.5 but exhibit skeletal and lung tissue abnormalities. This gene, when mutated, is highly expressed in embryonal carcinomas, pluripotent germ cell tumors, and other cancers and is thought to play an important role in tumor progression. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2017].
Disorder MIM:	614125

mRNA and Protein(s)

mRNA	Protein	Name
XM_011512954.2	XP_011511256.1	developmental pluripotency-associated protein 4 isoform X1
XM_011512954.3	XP_011511256.1	developmental pluripotency-associated protein 4 isoform X1
NM_001348928.1	NP_001335857.1	developmental pluripotency-associated protein 4 isoform 2
XM_024453622.1	XP_024309390.1	developmental pluripotency-associated protein 4 isoform X1
NM_001348928.2	NP_001335857.1	developmental pluripotency-associated protein 4 isoform 2
NM_001348929.1	NP_001335858.1	developmental pluripotency-associated protein 4 isoform 3
NM_018189.3	NP_060659.3	developmental pluripotency-associated protein 4 isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DPPA4	XP_516640.2
Homo sapiens (human)	DPPA4	NP_060659.3
Rattus norvegicus (Norway rat)	LOC685378	XP_001063552.2
	LOC706631	XP_001102141.2
Mus musculus (house mouse)	Dppa4	NP_082886.2

Gene Ontology
Bibliography

Related articles in PubMed

ERBB3-Binding Protein (EBP1) is a novel DPPA4 cofactor in human pluripotent cells.
Somanath P, Bush KM, Knoepfler PS
Stem cells (Dayton, Ohio)(2018 Jan)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

An inter-species protein-protein interaction network across vast evolutionary distance.
Zhong Q, Pevzner SJ, Hao T, Wang Y, Mosca R, Menche J, Taipale M, Ta?an M, Fan C, Yang X, Haley P, Murray RR, Mer F, Gebreab F, Tam S, MacWilliams A, Dricot A, Reichert P, Santhanam B, Ghamsari L, Calderwood MA, Rolland T, Charloteaux B, Lindquist S, Barab?si AL, Hill DE, Aloy P, Cusick ME, Xia Y, Roth FP, Vidal M
Molecular systems biology12(4)865(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

knockdown of PCGF1 results in reduced expression of DPPA4 and other subunits of the variant PRC1 complex at both mRNA and protein levels
Title: The variant Polycomb Repressor Complex 1 component PCGF1 interacts with a pluripotency sub-network that includes DPPA4, a regulator of embryogenesis.

Results identified a new family of pluripotency-related oncogenes consisting of DPPA2 and DPPA4.
Title: Identification of DPPA4 and DPPA2 as a novel family of pluripotency-related oncogenes.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

Thsi study identifies DPPA4 and other genes as putative Sox2:Oct-3/4 target genes using a combination of in silico analysis and transcription-based assays.
Title: Identification of DPPA4 and other genes as putative Sox2:Oct-3/4 target genes using a combination of in silico analysis and transcription-based assays.

The following DPPA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DPPA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02724	NM_018189.3 Latest version!	Homo sapiens developmental pluripotency associated 4 (DPPA4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu69176	NM_001348928.2 Latest version!	Homo sapiens developmental pluripotency associated 4 (DPPA4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu69176	XM_011512954.3 Latest version!	Homo sapiens developmental pluripotency associated 4 (DPPA4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu69176	XM_024453622.1 Latest version!	Homo sapiens developmental pluripotency associated 4 (DPPA4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu100933	NM_001348929.1 Latest version!	Homo sapiens developmental pluripotency associated 4 (DPPA4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu69176	XM_011512954.2	Homo sapiens developmental pluripotency associated 4 (DPPA4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu69176	NM_001348928.1	Homo sapiens developmental pluripotency associated 4 (DPPA4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DPPA4 ( NM_018189.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060659.3 Homo sapiens developmental pluripotency associated 4 (DPPA4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02724
Clone ID Related Accession (Same CDS sequence)	NM_018189.3
Accession Version	NM_018189.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	developmental pluripotency-associated protein 4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC076971.25, BC032846.2 and AA224162.1. This sequence is a reference standard in the RefSeqGene project. On Apr 11, 2007 this sequence version replaced NM_018189.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK001575.1, AK022698.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTGCGAG GCTCCGCTTC TTCTACAAGT ATGGAGAAGG CAAAAGGCAA GGAGTGGACC 
TCCACAGAGA AGTCGAGGGA AGAGGATCAG CAGGCTTCTA ATCAACCAAA TTCAATTGCT 
TTGCCAGGAA CATCAGCAAA GAGAACCAAA GAAAAAATGT CTATCAAAGG CAGTAAAGTG 
CTCTGCCCTA AGAAAAAGGC AGAGCACACT GACAACCCCA GACCTCAGAA GAAGATACCA 
ATCCCTCCAT TACCTTCTAA ACTGCCACCT GTTAATCTGA TTCACCGGGA CATTCTGCGG 
GCCTGGTGCC AACAATTGAA GCTGAGCTCC AAAGGCCAGA AATTGGATGC ATATAAGCGC 
CTGTGTGCCT TTGCCTACCC AAATCAAAAG GATTTTCCTA GCACAGCAAA AGAGGCCAAA 
ATCCGGAAAT CATTGCAAAA AAAATTAAAG GTGGAAAAGG GGGAAACGTC CCTGCAAAGT 
TCTGAGACAC ATCCTCCTGA AGTGGCTCTT CCTCCTGTGG GGGAGCCGCC TGCCCTGGAA 
AATTCCACTG CTCTCCTTGA GGGAGTTAAT ACAGTTGTGG TGACAACTTC TGCCCCAGAG 
GCTTTGCTGG CCTCCTGGGC GAGAATTTCA GCCAGGGCGA GGACACCAGA GGCAGTGGAA 
TCTCCACAAG AGGCCTCTGG TGTCAGGTGG TGTGTGGTCC ATGGGAAAAG TCTCCCTGCA 
GACACAGATG GTTGGGTTCA CCTGCAGTTT CATGCTGGTC AAGCCTGGGT TCCAGAAAAG 
CAAGAAGGGA GAGTGAGTGC ACTCTTCTTG CTTCCTGCCT CCAATTTTCC ACCCCCGCAC 
CTTGAAGACA ATATGTTGTG CCCCAAATGT GTTCACAGGA ACAAGGTCTT AATAAAAAGC 
CTCCAATGGG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060659.3
CDS	56..970
Translation	MLRGSASSTSMEKAKGKEWTSTEKSREEDQQASNQPNSIALPGTSAKRTKEKMSIKGSKVLCPKKKAEHTDNPRPQKKIPIPPLPSKLPPVNLIHRDILRAWCQQLKLSSKGQKLDAYKRLCAFAYPNQKDFPSTAKEAKIRKSLQKKLKVEKGETSLQSSETHPPEVALPPVGEPPALENSTALLEGVNTVVVTTSAPEALLASWARISARARTPEAVESPQEASGVRWCVVHGKSLPADTDGWVHLQFHAGQAWVPEKQEGRVSALFLLPASNFPPPHLEDNMLCPKCVHRNKVLIKSLQWE

Target ORF information:

RefSeq Version	NM_018189.3
Organism	Homo sapiens(human)
Definition	Homo sapiens developmental pluripotency associated 4 (DPPA4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018189.3

ORF Insert Sequence:

ATGTTGCGAG GCTCCGCTTC TTCTACAAGT ATGGAGAAGG CAAAAGGCAA GGAGTGGACC 
TCCACAGAGA AGTCGAGGGA AGAGGATCAG CAGGCTTCTA ATCAACCAAA TTCAATTGCT 
TTGCCAGGAA CATCAGCAAA GAGAACCAAA GAAAAAATGT CTATCAAAGG CAGTAAAGTG 
CTCTGCCCTA AGAAAAAGGC AGAGCACACT GACAACCCCA GACCTCAGAA GAAGATACCA 
ATCCCTCCAT TACCTTCTAA ACTGCCACCT GTTAATCTGA TTCACCGGGA CATTCTGCGG 
GCCTGGTGCC AACAATTGAA GCTGAGCTCC AAAGGCCAGA AATTGGATGC ATATAAGCGC 
CTGTGTGCCT TTGCCTACCC AAATCAAAAG GATTTTCCTA GCACAGCAAA AGAGGCCAAA 
ATCCGGAAAT CATTGCAAAA AAAATTAAAG GTGGAAAAGG GGGAAACGTC CCTGCAAAGT 
TCTGAGACAC ATCCTCCTGA AGTGGCTCTT CCTCCTGTGG GGGAGCCGCC TGCCCTGGAA 
AATTCCACTG CTCTCCTTGA GGGAGTTAAT ACAGTTGTGG TGACAACTTC TGCCCCAGAG 
GCTTTGCTGG CCTCCTGGGC GAGAATTTCA GCCAGGGCGA GGACACCAGA GGCAGTGGAA 
TCTCCACAAG AGGCCTCTGG TGTCAGGTGG TGTGTGGTCC ATGGGAAAAG TCTCCCTGCA 
GACACAGATG GTTGGGTTCA CCTGCAGTTT CATGCTGGTC AAGCCTGGGT TCCAGAAAAG 
CAAGAAGGGA GAGTGAGTGC ACTCTTCTTG CTTCCTGCCT CCAATTTTCC ACCCCCGCAC 
CTTGAAGACA ATATGTTGTG CCCCAAATGT GTTCACAGGA ACAAGGTCTT AATAAAAAGC 
CTCCAATGGG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPPA4 ( NM_001348928.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335857.1 Homo sapiens developmental pluripotency associated 4 (DPPA4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu69176
Clone ID Related Accession (Same CDS sequence)	XM_011512954.2 , XM_011512954.3 , NM_001348928.1 , XM_024453622.1 , NM_001348928.2
Accession Version	NM_001348928.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-16
Organism	Homo sapiens(human)
Product	developmental pluripotency-associated protein 4 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC076971.25 and AA224162.1. Transcript Variant: This variant (2) uses an alternate splice site in the 5' region and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DA730945.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2150385, SAMEA2153307 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTATCA AAGGCAGTAA AGTGCTCTGC CCTAAGAAAA AGGCAGAGCA CACTGACAAC 
CCCAGACCTC AGAAGAAGAT ACCAATCCCT CCATTACCTT CTAAACTGCC ACCTGTTAAT 
CTGATTCACC GGGACATTCT GCGGGCCTGG TGCCAACAAT TGAAGCTGAG CTCCAAAGGC 
CAGAAATTGG ATGCATATAA GCGCCTGTGT GCCTTTGCCT ACCCAAATCA AAAGGATTTT 
CCTAGCACAG CAAAAGAGGC CAAAATCCGG AAATCATTGC AAAAAAAATT AAAGGTGGAA 
AAGGGGGAAA CGTCCCTGCA AAGTTCTGAG ACACATCCTC CTGAAGTGGC TCTTCCTCCT 
GTGGGGGAGC CGCCTGCCCT GGAAAATTCC ACTGCTCTCC TTGAGGGAGT TAATACAGTT 
GTGGTGACAA CTTCTGCCCC AGAGGCTTTG CTGGCCTCCT GGGCGAGAAT TTCAGCCAGG 
GCGAGGACAC CAGAGGCAGT GGAATCTCCA CAAGAGGCCT CTGGTGTCAG GTGGTGTGTG 
GTCCATGGGA AAAGTCTCCC TGCAGACACA GATGGTTGGG TTCACCTGCA GTTTCATGCT 
GGTCAAGCCT GGGTTCCAGA AAAGCAAGAA GGGAGAGTGA GTGCACTCTT CTTGCTTCCT 
GCCTCCAATT TTCCACCCCC GCACCTTGAA GACAATATGT TGTGCCCCAA ATGTGTTCAC 
AGGAACAAGG TCTTAATAAA AAGCCTCCAA TGGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335857.1
CDS	139..897
Translation	MSIKGSKVLCPKKKAEHTDNPRPQKKIPIPPLPSKLPPVNLIHRDILRAWCQQLKLSSKGQKLDAYKRLCAFAYPNQKDFPSTAKEAKIRKSLQKKLKVEKGETSLQSSETHPPEVALPPVGEPPALENSTALLEGVNTVVVTTSAPEALLASWARISARARTPEAVESPQEASGVRWCVVHGKSLPADTDGWVHLQFHAGQAWVPEKQEGRVSALFLLPASNFPPPHLEDNMLCPKCVHRNKVLIKSLQWE

Target ORF information:

RefSeq Version	NM_001348928.1
Organism	Homo sapiens(human)
Definition	Homo sapiens developmental pluripotency associated 4 (DPPA4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348928.1

ORF Insert Sequence:

ATGTCTATCA AAGGCAGTAA AGTGCTCTGC CCTAAGAAAA AGGCAGAGCA CACTGACAAC 
CCCAGACCTC AGAAGAAGAT ACCAATCCCT CCATTACCTT CTAAACTGCC ACCTGTTAAT 
CTGATTCACC GGGACATTCT GCGGGCCTGG TGCCAACAAT TGAAGCTGAG CTCCAAAGGC 
CAGAAATTGG ATGCATATAA GCGCCTGTGT GCCTTTGCCT ACCCAAATCA AAAGGATTTT 
CCTAGCACAG CAAAAGAGGC CAAAATCCGG AAATCATTGC AAAAAAAATT AAAGGTGGAA 
AAGGGGGAAA CGTCCCTGCA AAGTTCTGAG ACACATCCTC CTGAAGTGGC TCTTCCTCCT 
GTGGGGGAGC CGCCTGCCCT GGAAAATTCC ACTGCTCTCC TTGAGGGAGT TAATACAGTT 
GTGGTGACAA CTTCTGCCCC AGAGGCTTTG CTGGCCTCCT GGGCGAGAAT TTCAGCCAGG 
GCGAGGACAC CAGAGGCAGT GGAATCTCCA CAAGAGGCCT CTGGTGTCAG GTGGTGTGTG 
GTCCATGGGA AAAGTCTCCC TGCAGACACA GATGGTTGGG TTCACCTGCA GTTTCATGCT 
GGTCAAGCCT GGGTTCCAGA AAAGCAAGAA GGGAGAGTGA GTGCACTCTT CTTGCTTCCT 
GCCTCCAATT TTCCACCCCC GCACCTTGAA GACAATATGT TGTGCCCCAA ATGTGTTCAC 
AGGAACAAGG TCTTAATAAA AAGCCTCCAA TGGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPPA4 ( NM_001348928.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335857.1 Homo sapiens developmental pluripotency associated 4 (DPPA4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu69176
Clone ID Related Accession (Same CDS sequence)	XM_011512954.2 , XM_011512954.3 , NM_001348928.1 , XM_024453622.1 , NM_001348928.2
Accession Version	NM_001348928.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	developmental pluripotency-associated protein 4 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC076971.25 and AA224162.1. On Jun 2, 2019 this sequence version replaced NM_001348928.1. Transcript Variant: This variant (2) uses an alternate splice site in the 5' region and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DA730945.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2150385, SAMEA2153307 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTATCA AAGGCAGTAA AGTGCTCTGC CCTAAGAAAA AGGCAGAGCA CACTGACAAC 
CCCAGACCTC AGAAGAAGAT ACCAATCCCT CCATTACCTT CTAAACTGCC ACCTGTTAAT 
CTGATTCACC GGGACATTCT GCGGGCCTGG TGCCAACAAT TGAAGCTGAG CTCCAAAGGC 
CAGAAATTGG ATGCATATAA GCGCCTGTGT GCCTTTGCCT ACCCAAATCA AAAGGATTTT 
CCTAGCACAG CAAAAGAGGC CAAAATCCGG AAATCATTGC AAAAAAAATT AAAGGTGGAA 
AAGGGGGAAA CGTCCCTGCA AAGTTCTGAG ACACATCCTC CTGAAGTGGC TCTTCCTCCT 
GTGGGGGAGC CGCCTGCCCT GGAAAATTCC ACTGCTCTCC TTGAGGGAGT TAATACAGTT 
GTGGTGACAA CTTCTGCCCC AGAGGCTTTG CTGGCCTCCT GGGCGAGAAT TTCAGCCAGG 
GCGAGGACAC CAGAGGCAGT GGAATCTCCA CAAGAGGCCT CTGGTGTCAG GTGGTGTGTG 
GTCCATGGGA AAAGTCTCCC TGCAGACACA GATGGTTGGG TTCACCTGCA GTTTCATGCT 
GGTCAAGCCT GGGTTCCAGA AAAGCAAGAA GGGAGAGTGA GTGCACTCTT CTTGCTTCCT 
GCCTCCAATT TTCCACCCCC GCACCTTGAA GACAATATGT TGTGCCCCAA ATGTGTTCAC 
AGGAACAAGG TCTTAATAAA AAGCCTCCAA TGGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335857.1
CDS	84..842
Translation	MSIKGSKVLCPKKKAEHTDNPRPQKKIPIPPLPSKLPPVNLIHRDILRAWCQQLKLSSKGQKLDAYKRLCAFAYPNQKDFPSTAKEAKIRKSLQKKLKVEKGETSLQSSETHPPEVALPPVGEPPALENSTALLEGVNTVVVTTSAPEALLASWARISARARTPEAVESPQEASGVRWCVVHGKSLPADTDGWVHLQFHAGQAWVPEKQEGRVSALFLLPASNFPPPHLEDNMLCPKCVHRNKVLIKSLQWE

Target ORF information:

RefSeq Version	NM_001348928.2
Organism	Homo sapiens(human)
Definition	Homo sapiens developmental pluripotency associated 4 (DPPA4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348928.2

ORF Insert Sequence:

ATGTCTATCA AAGGCAGTAA AGTGCTCTGC CCTAAGAAAA AGGCAGAGCA CACTGACAAC 
CCCAGACCTC AGAAGAAGAT ACCAATCCCT CCATTACCTT CTAAACTGCC ACCTGTTAAT 
CTGATTCACC GGGACATTCT GCGGGCCTGG TGCCAACAAT TGAAGCTGAG CTCCAAAGGC 
CAGAAATTGG ATGCATATAA GCGCCTGTGT GCCTTTGCCT ACCCAAATCA AAAGGATTTT 
CCTAGCACAG CAAAAGAGGC CAAAATCCGG AAATCATTGC AAAAAAAATT AAAGGTGGAA 
AAGGGGGAAA CGTCCCTGCA AAGTTCTGAG ACACATCCTC CTGAAGTGGC TCTTCCTCCT 
GTGGGGGAGC CGCCTGCCCT GGAAAATTCC ACTGCTCTCC TTGAGGGAGT TAATACAGTT 
GTGGTGACAA CTTCTGCCCC AGAGGCTTTG CTGGCCTCCT GGGCGAGAAT TTCAGCCAGG 
GCGAGGACAC CAGAGGCAGT GGAATCTCCA CAAGAGGCCT CTGGTGTCAG GTGGTGTGTG 
GTCCATGGGA AAAGTCTCCC TGCAGACACA GATGGTTGGG TTCACCTGCA GTTTCATGCT 
GGTCAAGCCT GGGTTCCAGA AAAGCAAGAA GGGAGAGTGA GTGCACTCTT CTTGCTTCCT 
GCCTCCAATT TTCCACCCCC GCACCTTGAA GACAATATGT TGTGCCCCAA ATGTGTTCAC 
AGGAACAAGG TCTTAATAAA AAGCCTCCAA TGGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPPA4 ( XM_011512954.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011511256.1 Homo sapiens developmental pluripotency associated 4 (DPPA4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu69176
Clone ID Related Accession (Same CDS sequence)	XM_011512954.2 , XM_011512954.3 , NM_001348928.1 , XM_024453622.1 , NM_001348928.2
Accession Version	XM_011512954.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	developmental pluripotency-associated protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512954.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTATCA AAGGCAGTAA AGTGCTCTGC CCTAAGAAAA AGGCAGAGCA CACTGACAAC 
CCCAGACCTC AGAAGAAGAT ACCAATCCCT CCATTACCTT CTAAACTGCC ACCTGTTAAT 
CTGATTCACC GGGACATTCT GCGGGCCTGG TGCCAACAAT TGAAGCTGAG CTCCAAAGGC 
CAGAAATTGG ATGCATATAA GCGCCTGTGT GCCTTTGCCT ACCCAAATCA AAAGGATTTT 
CCTAGCACAG CAAAAGAGGC CAAAATCCGG AAATCATTGC AAAAAAAATT AAAGGTGGAA 
AAGGGGGAAA CGTCCCTGCA AAGTTCTGAG ACACATCCTC CTGAAGTGGC TCTTCCTCCT 
GTGGGGGAGC CGCCTGCCCT GGAAAATTCC ACTGCTCTCC TTGAGGGAGT TAATACAGTT 
GTGGTGACAA CTTCTGCCCC AGAGGCTTTG CTGGCCTCCT GGGCGAGAAT TTCAGCCAGG 
GCGAGGACAC CAGAGGCAGT GGAATCTCCA CAAGAGGCCT CTGGTGTCAG GTGGTGTGTG 
GTCCATGGGA AAAGTCTCCC TGCAGACACA GATGGTTGGG TTCACCTGCA GTTTCATGCT 
GGTCAAGCCT GGGTTCCAGA AAAGCAAGAA GGGAGAGTGA GTGCACTCTT CTTGCTTCCT 
GCCTCCAATT TTCCACCCCC GCACCTTGAA GACAATATGT TGTGCCCCAA ATGTGTTCAC 
AGGAACAAGG TCTTAATAAA AAGCCTCCAA TGGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011511256.1
CDS	491..1249
Translation	MSIKGSKVLCPKKKAEHTDNPRPQKKIPIPPLPSKLPPVNLIHRDILRAWCQQLKLSSKGQKLDAYKRLCAFAYPNQKDFPSTAKEAKIRKSLQKKLKVEKGETSLQSSETHPPEVALPPVGEPPALENSTALLEGVNTVVVTTSAPEALLASWARISARARTPEAVESPQEASGVRWCVVHGKSLPADTDGWVHLQFHAGQAWVPEKQEGRVSALFLLPASNFPPPHLEDNMLCPKCVHRNKVLIKSLQWE

Target ORF information:

RefSeq Version	XM_011512954.2
Organism	Homo sapiens(human)
Definition	Homo sapiens developmental pluripotency associated 4 (DPPA4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512954.2

ORF Insert Sequence:

ATGTCTATCA AAGGCAGTAA AGTGCTCTGC CCTAAGAAAA AGGCAGAGCA CACTGACAAC 
CCCAGACCTC AGAAGAAGAT ACCAATCCCT CCATTACCTT CTAAACTGCC ACCTGTTAAT 
CTGATTCACC GGGACATTCT GCGGGCCTGG TGCCAACAAT TGAAGCTGAG CTCCAAAGGC 
CAGAAATTGG ATGCATATAA GCGCCTGTGT GCCTTTGCCT ACCCAAATCA AAAGGATTTT 
CCTAGCACAG CAAAAGAGGC CAAAATCCGG AAATCATTGC AAAAAAAATT AAAGGTGGAA 
AAGGGGGAAA CGTCCCTGCA AAGTTCTGAG ACACATCCTC CTGAAGTGGC TCTTCCTCCT 
GTGGGGGAGC CGCCTGCCCT GGAAAATTCC ACTGCTCTCC TTGAGGGAGT TAATACAGTT 
GTGGTGACAA CTTCTGCCCC AGAGGCTTTG CTGGCCTCCT GGGCGAGAAT TTCAGCCAGG 
GCGAGGACAC CAGAGGCAGT GGAATCTCCA CAAGAGGCCT CTGGTGTCAG GTGGTGTGTG 
GTCCATGGGA AAAGTCTCCC TGCAGACACA GATGGTTGGG TTCACCTGCA GTTTCATGCT 
GGTCAAGCCT GGGTTCCAGA AAAGCAAGAA GGGAGAGTGA GTGCACTCTT CTTGCTTCCT 
GCCTCCAATT TTCCACCCCC GCACCTTGAA GACAATATGT TGTGCCCCAA ATGTGTTCAC 
AGGAACAAGG TCTTAATAAA AAGCCTCCAA TGGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPPA4 ( XM_011512954.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011511256.1 Homo sapiens developmental pluripotency associated 4 (DPPA4), transcript variant X1, mRNA.

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CloneID	OHu69176
Clone ID Related Accession (Same CDS sequence)	XM_011512954.2 , XM_011512954.3 , NM_001348928.1 , XM_024453622.1 , NM_001348928.2
Accession Version	XM_011512954.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	developmental pluripotency-associated protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011512954.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTATCA AAGGCAGTAA AGTGCTCTGC CCTAAGAAAA AGGCAGAGCA CACTGACAAC 
CCCAGACCTC AGAAGAAGAT ACCAATCCCT CCATTACCTT CTAAACTGCC ACCTGTTAAT 
CTGATTCACC GGGACATTCT GCGGGCCTGG TGCCAACAAT TGAAGCTGAG CTCCAAAGGC 
CAGAAATTGG ATGCATATAA GCGCCTGTGT GCCTTTGCCT ACCCAAATCA AAAGGATTTT 
CCTAGCACAG CAAAAGAGGC CAAAATCCGG AAATCATTGC AAAAAAAATT AAAGGTGGAA 
AAGGGGGAAA CGTCCCTGCA AAGTTCTGAG ACACATCCTC CTGAAGTGGC TCTTCCTCCT 
GTGGGGGAGC CGCCTGCCCT GGAAAATTCC ACTGCTCTCC TTGAGGGAGT TAATACAGTT 
GTGGTGACAA CTTCTGCCCC AGAGGCTTTG CTGGCCTCCT GGGCGAGAAT TTCAGCCAGG 
GCGAGGACAC CAGAGGCAGT GGAATCTCCA CAAGAGGCCT CTGGTGTCAG GTGGTGTGTG 
GTCCATGGGA AAAGTCTCCC TGCAGACACA GATGGTTGGG TTCACCTGCA GTTTCATGCT 
GGTCAAGCCT GGGTTCCAGA AAAGCAAGAA GGGAGAGTGA GTGCACTCTT CTTGCTTCCT 
GCCTCCAATT TTCCACCCCC GCACCTTGAA GACAATATGT TGTGCCCCAA ATGTGTTCAC 
AGGAACAAGG TCTTAATAAA AAGCCTCCAA TGGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011511256.1
CDS	491..1249
Translation	MSIKGSKVLCPKKKAEHTDNPRPQKKIPIPPLPSKLPPVNLIHRDILRAWCQQLKLSSKGQKLDAYKRLCAFAYPNQKDFPSTAKEAKIRKSLQKKLKVEKGETSLQSSETHPPEVALPPVGEPPALENSTALLEGVNTVVVTTSAPEALLASWARISARARTPEAVESPQEASGVRWCVVHGKSLPADTDGWVHLQFHAGQAWVPEKQEGRVSALFLLPASNFPPPHLEDNMLCPKCVHRNKVLIKSLQWE

Target ORF information:

RefSeq Version	XM_011512954.3
Organism	Homo sapiens(human)
Definition	Homo sapiens developmental pluripotency associated 4 (DPPA4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512954.3

ORF Insert Sequence:

ATGTCTATCA AAGGCAGTAA AGTGCTCTGC CCTAAGAAAA AGGCAGAGCA CACTGACAAC 
CCCAGACCTC AGAAGAAGAT ACCAATCCCT CCATTACCTT CTAAACTGCC ACCTGTTAAT 
CTGATTCACC GGGACATTCT GCGGGCCTGG TGCCAACAAT TGAAGCTGAG CTCCAAAGGC 
CAGAAATTGG ATGCATATAA GCGCCTGTGT GCCTTTGCCT ACCCAAATCA AAAGGATTTT 
CCTAGCACAG CAAAAGAGGC CAAAATCCGG AAATCATTGC AAAAAAAATT AAAGGTGGAA 
AAGGGGGAAA CGTCCCTGCA AAGTTCTGAG ACACATCCTC CTGAAGTGGC TCTTCCTCCT 
GTGGGGGAGC CGCCTGCCCT GGAAAATTCC ACTGCTCTCC TTGAGGGAGT TAATACAGTT 
GTGGTGACAA CTTCTGCCCC AGAGGCTTTG CTGGCCTCCT GGGCGAGAAT TTCAGCCAGG 
GCGAGGACAC CAGAGGCAGT GGAATCTCCA CAAGAGGCCT CTGGTGTCAG GTGGTGTGTG 
GTCCATGGGA AAAGTCTCCC TGCAGACACA GATGGTTGGG TTCACCTGCA GTTTCATGCT 
GGTCAAGCCT GGGTTCCAGA AAAGCAAGAA GGGAGAGTGA GTGCACTCTT CTTGCTTCCT 
GCCTCCAATT TTCCACCCCC GCACCTTGAA GACAATATGT TGTGCCCCAA ATGTGTTCAC 
AGGAACAAGG TCTTAATAAA AAGCCTCCAA TGGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPPA4 ( XM_024453622.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309390.1 Homo sapiens developmental pluripotency associated 4 (DPPA4), transcript variant X2, mRNA.

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CloneID	OHu69176
Clone ID Related Accession (Same CDS sequence)	XM_011512954.2 , XM_011512954.3 , NM_001348928.1 , XM_024453622.1 , NM_001348928.2
Accession Version	XM_024453622.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	developmental pluripotency-associated protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTATCA AAGGCAGTAA AGTGCTCTGC CCTAAGAAAA AGGCAGAGCA CACTGACAAC 
CCCAGACCTC AGAAGAAGAT ACCAATCCCT CCATTACCTT CTAAACTGCC ACCTGTTAAT 
CTGATTCACC GGGACATTCT GCGGGCCTGG TGCCAACAAT TGAAGCTGAG CTCCAAAGGC 
CAGAAATTGG ATGCATATAA GCGCCTGTGT GCCTTTGCCT ACCCAAATCA AAAGGATTTT 
CCTAGCACAG CAAAAGAGGC CAAAATCCGG AAATCATTGC AAAAAAAATT AAAGGTGGAA 
AAGGGGGAAA CGTCCCTGCA AAGTTCTGAG ACACATCCTC CTGAAGTGGC TCTTCCTCCT 
GTGGGGGAGC CGCCTGCCCT GGAAAATTCC ACTGCTCTCC TTGAGGGAGT TAATACAGTT 
GTGGTGACAA CTTCTGCCCC AGAGGCTTTG CTGGCCTCCT GGGCGAGAAT TTCAGCCAGG 
GCGAGGACAC CAGAGGCAGT GGAATCTCCA CAAGAGGCCT CTGGTGTCAG GTGGTGTGTG 
GTCCATGGGA AAAGTCTCCC TGCAGACACA GATGGTTGGG TTCACCTGCA GTTTCATGCT 
GGTCAAGCCT GGGTTCCAGA AAAGCAAGAA GGGAGAGTGA GTGCACTCTT CTTGCTTCCT 
GCCTCCAATT TTCCACCCCC GCACCTTGAA GACAATATGT TGTGCCCCAA ATGTGTTCAC 
AGGAACAAGG TCTTAATAAA AAGCCTCCAA TGGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309390.1
CDS	125..883
Translation	MSIKGSKVLCPKKKAEHTDNPRPQKKIPIPPLPSKLPPVNLIHRDILRAWCQQLKLSSKGQKLDAYKRLCAFAYPNQKDFPSTAKEAKIRKSLQKKLKVEKGETSLQSSETHPPEVALPPVGEPPALENSTALLEGVNTVVVTTSAPEALLASWARISARARTPEAVESPQEASGVRWCVVHGKSLPADTDGWVHLQFHAGQAWVPEKQEGRVSALFLLPASNFPPPHLEDNMLCPKCVHRNKVLIKSLQWE

Target ORF information:

RefSeq Version	XM_024453622.1
Organism	Homo sapiens(human)
Definition	Homo sapiens developmental pluripotency associated 4 (DPPA4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453622.1

ORF Insert Sequence:

ATGTCTATCA AAGGCAGTAA AGTGCTCTGC CCTAAGAAAA AGGCAGAGCA CACTGACAAC 
CCCAGACCTC AGAAGAAGAT ACCAATCCCT CCATTACCTT CTAAACTGCC ACCTGTTAAT 
CTGATTCACC GGGACATTCT GCGGGCCTGG TGCCAACAAT TGAAGCTGAG CTCCAAAGGC 
CAGAAATTGG ATGCATATAA GCGCCTGTGT GCCTTTGCCT ACCCAAATCA AAAGGATTTT 
CCTAGCACAG CAAAAGAGGC CAAAATCCGG AAATCATTGC AAAAAAAATT AAAGGTGGAA 
AAGGGGGAAA CGTCCCTGCA AAGTTCTGAG ACACATCCTC CTGAAGTGGC TCTTCCTCCT 
GTGGGGGAGC CGCCTGCCCT GGAAAATTCC ACTGCTCTCC TTGAGGGAGT TAATACAGTT 
GTGGTGACAA CTTCTGCCCC AGAGGCTTTG CTGGCCTCCT GGGCGAGAAT TTCAGCCAGG 
GCGAGGACAC CAGAGGCAGT GGAATCTCCA CAAGAGGCCT CTGGTGTCAG GTGGTGTGTG 
GTCCATGGGA AAAGTCTCCC TGCAGACACA GATGGTTGGG TTCACCTGCA GTTTCATGCT 
GGTCAAGCCT GGGTTCCAGA AAAGCAAGAA GGGAGAGTGA GTGCACTCTT CTTGCTTCCT 
GCCTCCAATT TTCCACCCCC GCACCTTGAA GACAATATGT TGTGCCCCAA ATGTGTTCAC 
AGGAACAAGG TCTTAATAAA AAGCCTCCAA TGGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPPA4 ( NM_001348929.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335858.1 Homo sapiens developmental pluripotency associated 4 (DPPA4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu100933
Clone ID Related Accession (Same CDS sequence)	NM_001348929.1
Accession Version	NM_001348929.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	developmental pluripotency-associated protein 4 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC076971.25, AK298623.1, BC032846.2 and AA224162.1. Transcript Variant: This variant (3) lacks an exon in the 3' coding region and differs in the 3' UTR, compared to variant 1. The encoded isoform (3) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298623.1, DC424208.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGCGAG GCTCCGCTTC TTCTACAAGT ATGGAGAAGG CAAAAGGCAA GGAGTGGACC 
TCCACAGAGA AGTCGAGGGA AGAGGATCAG CAGGCTTCTA ATCAACCAAA TTCAATTGCT 
TTGCCAGGAA CATCAGCAAA GAGAACCAAA GAAAAAATGT CTATCAAAGG CAGTAAAGTG 
CTCTGCCCTA AGAAAAAGGC AGAGCACACT GACAACCCCA GACCTCAGAA GAAGATACCA 
ATCCCTCCAT TACCTTCTAA ACTGCCACCT GTTAATCTGA TTCACCGGGA CATTCTGCGG 
GCCTGGTGCC AACAATTGAA GCTGAGCTCC AAAGGCCAGA AATTGGATGC ATATAAGCGC 
CTGTGTGCCT TTGCCTACCC AAATCAAAAG GTGGTGTGTG GTCCATGGGA AAAGTCTCCC 
TGCAGACACA GATGGTTGGG TTCACCTGCA GTTTCATGCT GGTCAAGCCT GGGTTCCAGA 
AAAGCAAGAA GGGAGAGTGA GTGCACTCTT CTTGCTTCCT GCCTCCAATT TTCCACCCCC 
GCACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335858.1
CDS	56..604
Translation	MLRGSASSTSMEKAKGKEWTSTEKSREEDQQASNQPNSIALPGTSAKRTKEKMSIKGSKVLCPKKKAEHTDNPRPQKKIPIPPLPSKLPPVNLIHRDILRAWCQQLKLSSKGQKLDAYKRLCAFAYPNQKVVCGPWEKSPCRHRWLGSPAVSCWSSLGSRKARRESECTLLASCLQFSTPAP

Target ORF information:

RefSeq Version	NM_001348929.1
Organism	Homo sapiens(human)
Definition	Homo sapiens developmental pluripotency associated 4 (DPPA4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348929.1

ORF Insert Sequence:

ATGTTGCGAG GCTCCGCTTC TTCTACAAGT ATGGAGAAGG CAAAAGGCAA GGAGTGGACC 
TCCACAGAGA AGTCGAGGGA AGAGGATCAG CAGGCTTCTA ATCAACCAAA TTCAATTGCT 
TTGCCAGGAA CATCAGCAAA GAGAACCAAA GAAAAAATGT CTATCAAAGG CAGTAAAGTG 
CTCTGCCCTA AGAAAAAGGC AGAGCACACT GACAACCCCA GACCTCAGAA GAAGATACCA 
ATCCCTCCAT TACCTTCTAA ACTGCCACCT GTTAATCTGA TTCACCGGGA CATTCTGCGG 
GCCTGGTGCC AACAATTGAA GCTGAGCTCC AAAGGCCAGA AATTGGATGC ATATAAGCGC 
CTGTGTGCCT TTGCCTACCC AAATCAAAAG GTGGTGTGTG GTCCATGGGA AAAGTCTCCC 
TGCAGACACA GATGGTTGGG TTCACCTGCA GTTTCATGCT GGTCAAGCCT GGGTTCCAGA 
AAAGCAAGAA GGGAGAGTGA GTGCACTCTT CTTGCTTCCT GCCTCCAATT TTCCACCCCC 
GCACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ERBB3-Binding Protein (EBP1) is a novel DPPA4 cofactor in human pluripotent cells.
Stem cells (Dayton, Ohio)(2018 Jan)
Somanath P,Bush KM,Knoepfler PS

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

An inter-species protein-protein interaction network across vast evolutionary distance.
Molecular systems biology12(4)865(2016 Apr)
Zhong Q,Pevzner SJ,Hao T,Wang Y,Mosca R,Menche J,Taipale M,Ta?an M,Fan C,Yang X,Haley P,Murray RR,Mer F,Gebreab F,Tam S,MacWilliams A,Dricot A,Reichert P,Santhanam B,Ghamsari L,Calderwood MA,Rolland T,Charloteaux B,Lindquist S,Barab?si AL,Hill DE,Aloy P,Cusick ME,Xia Y,Roth FP,Vidal M

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DPPA4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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