SASH3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SASH3
Entrez Gene ID	54440
Full Name	SAM and SH3 domain containing 3
Synonyms	753P9,CXorf9,HACS2,SH3D6C,SLY
General protein information	Preferred Names SAM and SH3 domain containing 3 Names SAM and SH3 domain-containing protein 3 SH3 protein expressed in lymphocytes homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq26.1
Summary	The protein encoded by this gene contains a Src homology-3 (SH3) domain and a sterile alpha motif (SAM), both of which are found in proteins involved in cell signaling. This protein may function as a signaling adapter protein in lymphocytes.[provided by RefSeq, Sep 2009].
Disorder MIM:	300441

mRNA and Protein(s)

mRNA	Protein	Name
NM_018990.3	NP_061863.1	SAM and SH3 domain-containing protein 3
NM_018990.4	NP_061863.1	SAM and SH3 domain-containing protein 3
XM_006724763.1	XP_006724826.1	SAM and SH3 domain-containing protein 3 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Sash3	NP_083049.1
Bos taurus (cattle)	SASH3	NP_001071466.1
Pan troglodytes (chimpanzee)	SASH3	XP_003954127.1
	SASH3	XP_001091319.1
Xenopus tropicalis (tropical clawed frog)	sash3	NP_001016498.1
Homo sapiens (human)	SASH3	NP_061863.1
Rattus norvegicus (Norway rat)	Sash3	NP_001128464.1
Danio rerio (zebrafish)	LOC561954	XP_690443.4
Canis lupus familiaris (dog)	SASH3	XP_005641847.1
Gallus gallus (chicken)	SASH3	NP_001026281.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput mapping of a dynamic signaling network in mammalian cells.
Barrios-Rodiles M, Brown KR, Ozdamar B, Bose R, Liu Z, Donovan RS, Shinjo F, Liu Y, Dembowy J, Taylor IW, Luga V, Przulj N, Robinson M, Suzuki H, Hayashizaki Y, Jurisica I, Wrana JL
Science (New York, N.Y.)307(5715)1621-5(2005 Mar)

Approaches to define antigen receptor-induced serine kinase signal transduction pathways.
Astoul E, Laurence AD, Totty N, Beer S, Alexander DR, Cantrell DA
The Journal of biological chemistry278(11)9267-75(2003 Mar)

Molecular cloning and characterization of a novel SH3 protein (SLY) preferentially expressed in lymphoid cells.
Beer S, Simins AB, Schuster A, Holzmann B
Biochimica et biophysica acta1520(1)89-93(2001 Jul)

Protein interactions regulating vesicle transport between the endoplasmic reticulum and Golgi apparatus in mammalian cells.
Hay JC, Chao DS, Kuo CS, Scheller RH
Cell89(1)149-58(1997 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SASH3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SASH3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19711	NM_018990.4 Latest version!	Homo sapiens SAM and SH3 domain containing 3 (SASH3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu54330	XM_006724763.1 Latest version!	Homo sapiens SAM and SH3 domain containing 3 (SASH3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu19711	NM_018990.3	Homo sapiens SAM and SH3 domain containing 3 (SASH3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SASH3 ( NM_018990.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_061863.1 Homo sapiens SAM and SH3 domain containing 3 (SASH3), mRNA.

Price & Availability↑

CloneID	OHu19711
Clone ID Related Accession (Same CDS sequence)	NM_018990.3 , NM_018990.4
Accession Version	NM_018990.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	SAM and SH3 domain-containing protein 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR975883.1 and BC051881.1. This sequence is a reference standard in the RefSeqGene project. On Jul 15, 2009 this sequence version replaced NM_018990.2. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.177044.1, SRR1163658.14617.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCGCC GCAAGCCCTC CAATGCCAGT GAGAAGGAGC CCACTCAGAA GAAAAAGCTC 
TCCCTTCAGC GCTCCAGCAG CTTCAAGGAT TTTGCCAAAT CCAAACCCAG CTCCCCCGTG 
GTGAGCGAGA AGGAGTTTAA TCTGGATGAT AACATTCCAG AAGATGACTC AGGTGTCCCC 
ACCCCAGAAG ATGCTGGGAA GAGTGGCAAA AAGCTGGGGA AGAAGTGGAG GGCAGTGATT 
TCCCGAACCA TGAACAGGAA GATGGGCAAG ATGATGGTGA AGGCCCTGTC AGAAGAGATG 
GCAGACACTC TGGAGGAGGG CTCTGCCTCC CCGACATCTC CAGACTACAG CCTGGACAGC 
CCTGGCCCTG AGAAGATGGC GCTGGCCTTT TCTGAGCAAG AGGAGCATGA ACTTCCGGTG 
CTCAGCCGCC AGGCATCAAC AGGCAGTGAG CTCTGCAGCC CCAGCCCAGG TTCTGGCAGC 
TTCGGGGAGG AACCACCTGC CCCCCAGTAC ACAGGGCCTT TCTGTGGCCG GGCACGAGTC 
CACACCGACT TCACTCCCAG CCCCTATGAC CACGACTCGC TGAAACTGCA GAAAGGAGAT 
GTGATCCAGA TCATTGAAAA GCCACCTGTG GGCACGTGGC TGGGCCTACT CAATGGCAAG 
GTGGGCTCTT TCAAATTCAT CTATGTGGAT GTGCTGCCCG AGGAGGCCGT GGGGCATGCC 
CGCCCCAGCC GCCGACAGAG CAAGGGCAAG AGGCCCAAGC CTAAGACCCT GCATGAGCTG 
CTGGAGCGCA TCGGCCTGGA GGAGCACACA TCCACCCTCC TGCTCAATGG CTACCAGACA 
CTGGAAGACT TCAAAGAGCT GCGAGAAACA CACCTCAATG AGCTGAACAT CATGGATCCA 
CAGCACCGGG CCAAGCTGCT CACGGCCGCC GAGCTGCTGC TGGACTATGA CACTGGCAGT 
GAGGAGGCTG AAGAGGGCGC CGAGAGCAGC CAGGAGCCAG TGGCACACAC AGTGTCGGAA 
CCCAAGGTGG ACATCCCGCG CGACTCAGGC TGCTTTGAGG GCTCGGAGAG CGGGCGCGAT 
GACGCAGAGC TGGCAGGCAC TGAGGAGCAG CTGCAAGGCC TCTCCCTGGC CGGGGCACCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061863.1
CDS	183..1325
Translation	MLRRKPSNASEKEPTQKKKLSLQRSSSFKDFAKSKPSSPVVSEKEFNLDDNIPEDDSGVPTPEDAGKSGKKLGKKWRAVISRTMNRKMGKMMVKALSEEMADTLEEGSASPTSPDYSLDSPGPEKMALAFSEQEEHELPVLSRQASTGSELCSPSPGSGSFGEEPPAPQYTGPFCGRARVHTDFTPSPYDHDSLKLQKGDVIQIIEKPPVGTWLGLLNGKVGSFKFIYVDVLPEEAVGHARPSRRQSKGKRPKPKTLHELLERIGLEEHTSTLLLNGYQTLEDFKELRETHLNELNIMDPQHRAKLLTAAELLLDYDTGSEEAEEGAESSQEPVAHTVSEPKVDIPRDSGCFEGSESGRDDAELAGTEEQLQGLSLAGAP

Target ORF information:

RefSeq Version	NM_018990.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SAM and SH3 domain containing 3 (SASH3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018990.3

ORF Insert Sequence:

ATGCTGCGCC GCAAGCCCTC CAATGCCAGT GAGAAGGAGC CCACTCAGAA GAAAAAGCTC 
TCCCTTCAGC GCTCCAGCAG CTTCAAGGAT TTTGCCAAAT CCAAACCCAG CTCCCCCGTG 
GTGAGCGAGA AGGAGTTTAA TCTGGATGAT AACATTCCAG AAGATGACTC AGGTGTCCCC 
ACCCCAGAAG ATGCTGGGAA GAGTGGCAAA AAGCTGGGGA AGAAGTGGAG GGCAGTGATT 
TCCCGAACCA TGAACAGGAA GATGGGCAAG ATGATGGTGA AGGCCCTGTC AGAAGAGATG 
GCAGACACTC TGGAGGAGGG CTCTGCCTCC CCGACATCTC CAGACTACAG CCTGGACAGC 
CCTGGCCCTG AGAAGATGGC GCTGGCCTTT TCTGAGCAAG AGGAGCATGA ACTTCCGGTG 
CTCAGCCGCC AGGCATCAAC AGGCAGTGAG CTCTGCAGCC CCAGCCCAGG TTCTGGCAGC 
TTCGGGGAGG AACCACCTGC CCCCCAGTAC ACAGGGCCTT TCTGTGGCCG GGCACGAGTC 
CACACCGACT TCACTCCCAG CCCCTATGAC CACGACTCGC TGAAACTGCA GAAAGGAGAT 
GTGATCCAGA TCATTGAAAA GCCACCTGTG GGCACGTGGC TGGGCCTACT CAATGGCAAG 
GTGGGCTCTT TCAAATTCAT CTATGTGGAT GTGCTGCCCG AGGAGGCCGT GGGGCATGCC 
CGCCCCAGCC GCCGACAGAG CAAGGGCAAG AGGCCCAAGC CTAAGACCCT GCATGAGCTG 
CTGGAGCGCA TCGGCCTGGA GGAGCACACA TCCACCCTCC TGCTCAATGG CTACCAGACA 
CTGGAAGACT TCAAAGAGCT GCGAGAAACA CACCTCAATG AGCTGAACAT CATGGATCCA 
CAGCACCGGG CCAAGCTGCT CACGGCCGCC GAGCTGCTGC TGGACTATGA CACTGGCAGT 
GAGGAGGCTG AAGAGGGCGC CGAGAGCAGC CAGGAGCCAG TGGCACACAC AGTGTCGGAA 
CCCAAGGTGG ACATCCCGCG CGACTCAGGC TGCTTTGAGG GCTCGGAGAG CGGGCGCGAT 
GACGCAGAGC TGGCAGGCAC TGAGGAGCAG CTGCAAGGCC TCTCCCTGGC CGGGGCACCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SASH3 ( NM_018990.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_061863.1 Homo sapiens SAM and SH3 domain containing 3 (SASH3), mRNA.

Price & Availability↑

CloneID	OHu19711
Clone ID Related Accession (Same CDS sequence)	NM_018990.3 , NM_018990.4
Accession Version	NM_018990.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	SAM and SH3 domain-containing protein 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR975883.1 and BC051881.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_018990.3. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.983144.1, SRR1163657.562370.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000356892.4/ ENSP00000349359.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGCGCC GCAAGCCCTC CAATGCCAGT GAGAAGGAGC CCACTCAGAA GAAAAAGCTC 
TCCCTTCAGC GCTCCAGCAG CTTCAAGGAT TTTGCCAAAT CCAAACCCAG CTCCCCCGTG 
GTGAGCGAGA AGGAGTTTAA TCTGGATGAT AACATTCCAG AAGATGACTC AGGTGTCCCC 
ACCCCAGAAG ATGCTGGGAA GAGTGGCAAA AAGCTGGGGA AGAAGTGGAG GGCAGTGATT 
TCCCGAACCA TGAACAGGAA GATGGGCAAG ATGATGGTGA AGGCCCTGTC AGAAGAGATG 
GCAGACACTC TGGAGGAGGG CTCTGCCTCC CCGACATCTC CAGACTACAG CCTGGACAGC 
CCTGGCCCTG AGAAGATGGC GCTGGCCTTT TCTGAGCAAG AGGAGCATGA ACTTCCGGTG 
CTCAGCCGCC AGGCATCAAC AGGCAGTGAG CTCTGCAGCC CCAGCCCAGG TTCTGGCAGC 
TTCGGGGAGG AACCACCTGC CCCCCAGTAC ACAGGGCCTT TCTGTGGCCG GGCACGAGTC 
CACACCGACT TCACTCCCAG CCCCTATGAC CACGACTCGC TGAAACTGCA GAAAGGAGAT 
GTGATCCAGA TCATTGAAAA GCCACCTGTG GGCACGTGGC TGGGCCTACT CAATGGCAAG 
GTGGGCTCTT TCAAATTCAT CTATGTGGAT GTGCTGCCCG AGGAGGCCGT GGGGCATGCC 
CGCCCCAGCC GCCGACAGAG CAAGGGCAAG AGGCCCAAGC CTAAGACCCT GCATGAGCTG 
CTGGAGCGCA TCGGCCTGGA GGAGCACACA TCCACCCTCC TGCTCAATGG CTACCAGACA 
CTGGAAGACT TCAAAGAGCT GCGAGAAACA CACCTCAATG AGCTGAACAT CATGGATCCA 
CAGCACCGGG CCAAGCTGCT CACGGCCGCC GAGCTGCTGC TGGACTATGA CACTGGCAGT 
GAGGAGGCTG AAGAGGGCGC CGAGAGCAGC CAGGAGCCAG TGGCACACAC AGTGTCGGAA 
CCCAAGGTGG ACATCCCGCG CGACTCAGGC TGCTTTGAGG GCTCGGAGAG CGGGCGCGAT 
GACGCAGAGC TGGCAGGCAC TGAGGAGCAG CTGCAAGGCC TCTCCCTGGC CGGGGCACCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061863.1
CDS	150..1292
Translation	MLRRKPSNASEKEPTQKKKLSLQRSSSFKDFAKSKPSSPVVSEKEFNLDDNIPEDDSGVPTPEDAGKSGKKLGKKWRAVISRTMNRKMGKMMVKALSEEMADTLEEGSASPTSPDYSLDSPGPEKMALAFSEQEEHELPVLSRQASTGSELCSPSPGSGSFGEEPPAPQYTGPFCGRARVHTDFTPSPYDHDSLKLQKGDVIQIIEKPPVGTWLGLLNGKVGSFKFIYVDVLPEEAVGHARPSRRQSKGKRPKPKTLHELLERIGLEEHTSTLLLNGYQTLEDFKELRETHLNELNIMDPQHRAKLLTAAELLLDYDTGSEEAEEGAESSQEPVAHTVSEPKVDIPRDSGCFEGSESGRDDAELAGTEEQLQGLSLAGAP

Target ORF information:

RefSeq Version	NM_018990.4
Organism	Homo sapiens(human)
Definition	Homo sapiens SAM and SH3 domain containing 3 (SASH3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018990.4

ORF Insert Sequence:

ATGCTGCGCC GCAAGCCCTC CAATGCCAGT GAGAAGGAGC CCACTCAGAA GAAAAAGCTC 
TCCCTTCAGC GCTCCAGCAG CTTCAAGGAT TTTGCCAAAT CCAAACCCAG CTCCCCCGTG 
GTGAGCGAGA AGGAGTTTAA TCTGGATGAT AACATTCCAG AAGATGACTC AGGTGTCCCC 
ACCCCAGAAG ATGCTGGGAA GAGTGGCAAA AAGCTGGGGA AGAAGTGGAG GGCAGTGATT 
TCCCGAACCA TGAACAGGAA GATGGGCAAG ATGATGGTGA AGGCCCTGTC AGAAGAGATG 
GCAGACACTC TGGAGGAGGG CTCTGCCTCC CCGACATCTC CAGACTACAG CCTGGACAGC 
CCTGGCCCTG AGAAGATGGC GCTGGCCTTT TCTGAGCAAG AGGAGCATGA ACTTCCGGTG 
CTCAGCCGCC AGGCATCAAC AGGCAGTGAG CTCTGCAGCC CCAGCCCAGG TTCTGGCAGC 
TTCGGGGAGG AACCACCTGC CCCCCAGTAC ACAGGGCCTT TCTGTGGCCG GGCACGAGTC 
CACACCGACT TCACTCCCAG CCCCTATGAC CACGACTCGC TGAAACTGCA GAAAGGAGAT 
GTGATCCAGA TCATTGAAAA GCCACCTGTG GGCACGTGGC TGGGCCTACT CAATGGCAAG 
GTGGGCTCTT TCAAATTCAT CTATGTGGAT GTGCTGCCCG AGGAGGCCGT GGGGCATGCC 
CGCCCCAGCC GCCGACAGAG CAAGGGCAAG AGGCCCAAGC CTAAGACCCT GCATGAGCTG 
CTGGAGCGCA TCGGCCTGGA GGAGCACACA TCCACCCTCC TGCTCAATGG CTACCAGACA 
CTGGAAGACT TCAAAGAGCT GCGAGAAACA CACCTCAATG AGCTGAACAT CATGGATCCA 
CAGCACCGGG CCAAGCTGCT CACGGCCGCC GAGCTGCTGC TGGACTATGA CACTGGCAGT 
GAGGAGGCTG AAGAGGGCGC CGAGAGCAGC CAGGAGCCAG TGGCACACAC AGTGTCGGAA 
CCCAAGGTGG ACATCCCGCG CGACTCAGGC TGCTTTGAGG GCTCGGAGAG CGGGCGCGAT 
GACGCAGAGC TGGCAGGCAC TGAGGAGCAG CTGCAAGGCC TCTCCCTGGC CGGGGCACCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SASH3 ( XM_006724763.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006724826.1 Homo sapiens SAM and SH3 domain containing 3 (SASH3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu54330
Clone ID Related Accession (Same CDS sequence)	XM_006724763.1
Accession Version	XM_006724763.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	SAM and SH3 domain-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGCC GCAAGCCCTC CAATGCCAGT GAGAAGGAGC CCACTCAGAA GAAAAAGCTC 
TCCCTTCAGC GCTCCAGCAG CTTCAAGGAT TTTGCCAAAT CCAAACCCAG CTCCCCCGTG 
GTGAGCGAGA AGGAGTTTAA TCTGGATGAT AACATTCCAG AAGATGACTC AGGTGTCCCC 
ACCCCAGAAG ATGCTGGGAA GAGTGGCAAA AAGCTGGGGA AGAAGTGGAG GGCAGTGATT 
TCCCGAACCA TGAACAGGAA GATGGGCAAG ATGATGGTGA AGGCCCTGTC AGAAGAGATG 
GCAGACACTC TGGAGGAGGG CTCTGCCTCC CCGACATCTC CAGACTACAG CCTGGACAGC 
CCTGGCCCTG AGAAGATGGC GCTGGCCTTT TCTGAGCAAG AGGAGCATGA ACTTCCGGTG 
CTCAGCCGCC AGGCATCAAC AGGCAGTGAG CTCTGCAGCC CCAGCCCAGG TTCTGGCAGC 
TTCGGGGAGG AACCACCTGC CCCCCAGTAC ACAGGGCCTT TCTGTGGCCG GGCACGAGTC 
CACACCGACT TCACTCCCAG CCCCTATGAC CACGACTCGC TGAAACTGCA GGTAAGATCA 
GCATCCGGGC TTCTCTGGAG CCTGGCAGGC TGTGCCCAAA AAGGAAGCTG GACTGAACCA 
GGCTATGACA GTGTCAGTGG AGGCCAAGGC CCCCCATATC CTCTCTCCCT TGCTCCCTTC 
TCCTCTCCTC TCCCCCAACA GAAAGGAGAT GTGATCCAGA TCATTGAAAA GCCACCTGTG 
GGCACGTGGC TGGGCCTACT CAATGGCAAG GTGGGCTCTT TCAAATTCAT CTATGTGGAT 
GTGCTGCCCG AGGAGGCCGT GGGGCATGCC CGCCCCAGCC GCCGACAGAG CAAGGGCAAG 
AGGCCCAAGC CTAAGACCCT GCATGAGCTG CTGGAGCGCA TCGGCCTGGA GGAGCACACA 
TCCACCCTCC TGCTCAATGG CTACCAGACA CTGGAAGACT TCAAAGAGCT GCGAGAAACA 
CACCTCAATG AGCTGAACAT CATGGATCCA CAGCACCGGG CCAAGCTGCT CACGGCCGCC 
GAGCTGCTGC TGGACTATGA CACTGGCAGT GAGGAGGCTG AAGAGGGCGC CGAGAGCAGC 
CAGGAGCCAG TGGCACACAC AGTGTCGGAA CCCAAGGTGG ACATCCCGCG CGACTCAGGC 
TGCTTTGAGG GCTCGGAGAG CGGGCGCGAT GACGCAGAGC TGGCAGGCAC TGAGGAGCAG 
CTGCAAGGCC TCTCCCTGGC CGGGGCACCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006724826.1
CDS	150..1442
Translation	MLRRKPSNASEKEPTQKKKLSLQRSSSFKDFAKSKPSSPVVSEKEFNLDDNIPEDDSGVPTPEDAGKSGKKLGKKWRAVISRTMNRKMGKMMVKALSEEMADTLEEGSASPTSPDYSLDSPGPEKMALAFSEQEEHELPVLSRQASTGSELCSPSPGSGSFGEEPPAPQYTGPFCGRARVHTDFTPSPYDHDSLKLQVRSASGLLWSLAGCAQKGSWTEPGYDSVSGGQGPPYPLSLAPFSSPLPQQKGDVIQIIEKPPVGTWLGLLNGKVGSFKFIYVDVLPEEAVGHARPSRRQSKGKRPKPKTLHELLERIGLEEHTSTLLLNGYQTLEDFKELRETHLNELNIMDPQHRAKLLTAAELLLDYDTGSEEAEEGAESSQEPVAHTVSEPKVDIPRDSGCFEGSESGRDDAELAGTEEQLQGLSLAGAP

Target ORF information:

RefSeq Version	XM_006724763.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SAM and SH3 domain containing 3 (SASH3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006724763.1

ORF Insert Sequence:

ATGCTGCGCC GCAAGCCCTC CAATGCCAGT GAGAAGGAGC CCACTCAGAA GAAAAAGCTC 
TCCCTTCAGC GCTCCAGCAG CTTCAAGGAT TTTGCCAAAT CCAAACCCAG CTCCCCCGTG 
GTGAGCGAGA AGGAGTTTAA TCTGGATGAT AACATTCCAG AAGATGACTC AGGTGTCCCC 
ACCCCAGAAG ATGCTGGGAA GAGTGGCAAA AAGCTGGGGA AGAAGTGGAG GGCAGTGATT 
TCCCGAACCA TGAACAGGAA GATGGGCAAG ATGATGGTGA AGGCCCTGTC AGAAGAGATG 
GCAGACACTC TGGAGGAGGG CTCTGCCTCC CCGACATCTC CAGACTACAG CCTGGACAGC 
CCTGGCCCTG AGAAGATGGC GCTGGCCTTT TCTGAGCAAG AGGAGCATGA ACTTCCGGTG 
CTCAGCCGCC AGGCATCAAC AGGCAGTGAG CTCTGCAGCC CCAGCCCAGG TTCTGGCAGC 
TTCGGGGAGG AACCACCTGC CCCCCAGTAC ACAGGGCCTT TCTGTGGCCG GGCACGAGTC 
CACACCGACT TCACTCCCAG CCCCTATGAC CACGACTCGC TGAAACTGCA GGTAAGATCA 
GCATCCGGGC TTCTCTGGAG CCTGGCAGGC TGTGCCCAAA AAGGAAGCTG GACTGAACCA 
GGCTATGACA GTGTCAGTGG AGGCCAAGGC CCCCCATATC CTCTCTCCCT TGCTCCCTTC 
TCCTCTCCTC TCCCCCAACA GAAAGGAGAT GTGATCCAGA TCATTGAAAA GCCACCTGTG 
GGCACGTGGC TGGGCCTACT CAATGGCAAG GTGGGCTCTT TCAAATTCAT CTATGTGGAT 
GTGCTGCCCG AGGAGGCCGT GGGGCATGCC CGCCCCAGCC GCCGACAGAG CAAGGGCAAG 
AGGCCCAAGC CTAAGACCCT GCATGAGCTG CTGGAGCGCA TCGGCCTGGA GGAGCACACA 
TCCACCCTCC TGCTCAATGG CTACCAGACA CTGGAAGACT TCAAAGAGCT GCGAGAAACA 
CACCTCAATG AGCTGAACAT CATGGATCCA CAGCACCGGG CCAAGCTGCT CACGGCCGCC 
GAGCTGCTGC TGGACTATGA CACTGGCAGT GAGGAGGCTG AAGAGGGCGC CGAGAGCAGC 
CAGGAGCCAG TGGCACACAC AGTGTCGGAA CCCAAGGTGG ACATCCCGCG CGACTCAGGC 
TGCTTTGAGG GCTCGGAGAG CGGGCGCGAT GACGCAGAGC TGGCAGGCAC TGAGGAGCAG 
CTGCAAGGCC TCTCCCTGGC CGGGGCACCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput mapping of a dynamic signaling network in mammalian cells.
Science (New York, N.Y.)307(5715)1621-5(2005 Mar)
Barrios-Rodiles M,Brown KR,Ozdamar B,Bose R,Liu Z,Donovan RS,Shinjo F,Liu Y,Dembowy J,Taylor IW,Luga V,Przulj N,Robinson M,Suzuki H,Hayashizaki Y,Jurisica I,Wrana JL

Approaches to define antigen receptor-induced serine kinase signal transduction pathways.
The Journal of biological chemistry278(11)9267-75(2003 Mar)
Astoul E,Laurence AD,Totty N,Beer S,Alexander DR,Cantrell DA

Molecular cloning and characterization of a novel SH3 protein (SLY) preferentially expressed in lymphoid cells.
Biochimica et biophysica acta1520(1)89-93(2001 Jul)
Beer S,Simins AB,Schuster A,Holzmann B

Protein interactions regulating vesicle transport between the endoplasmic reticulum and Golgi apparatus in mammalian cells.
Cell89(1)149-58(1997 Apr)
Hay JC,Chao DS,Kuo CS,Scheller RH