POLR2D cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLR2D
Entrez Gene ID	5433
Full Name	RNA polymerase II subunit D
Synonyms	HSRBP4,HSRPB4,RBP4,RPB16
General protein information	Preferred Names RNA polymerase II subunit D Names DNA-directed RNA polymerase II subunit RPB4 DNA-directed RNA polymerase II 16 kDa polypeptide DNA-directed RNA polymerase II subunit D RNA polymerase II 16 kDa subunit RNA polymerase II subunit B4 RNA polymerase II subunit hsRBP4 polymerase (RNA) II (DNA directed) polypeptide D polymerase (RNA) II subunit D
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q14.3
Summary	This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	606017

mRNA and Protein(s)

mRNA	Protein	Name
NM_004805.3	NP_004796.1	DNA-directed RNA polymerase II subunit RPB4
NM_004805.4	NP_004796.1	DNA-directed RNA polymerase II subunit RPB4

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Polr2d	NP_081278.1
Gallus gallus (chicken)	POLR2D	NP_001264338.1
Bos taurus (cattle)	POLR2D	NP_001069926.1
Drosophila melanogaster (fruit fly)	Rpb4	NP_001014633.2
Caenorhabditis elegans (roundworm)	rpb-4	NP_495544.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001228	XP_321930.4
Arabidopsis thaliana (thale cress)	NRPB4	NP_196554.1
Oryza sativa (rice)	Os02g0116900	NP_001045683.1
Rattus norvegicus (Norway rat)	Polr2d	NP_001102356.1
Xenopus tropicalis (tropical clawed frog)	polr2d	NP_001016248.1
Homo sapiens (human)	POLR2D	NP_004796.1
Pan troglodytes (chimpanzee)	POLR2D	NP_001238888.1
Canis lupus familiaris (dog)	POLR2D	XP_540983.2
Danio rerio (zebrafish)	polr2d	NP_001002317.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic polymorphisms in 85 DNA repair genes and bladder cancer risk.
Michiels S, Laplanche A, Boulet T, Dessen P, Guillonneau B, M?jean A, Desgrandchamps F, Lathrop M, Sarasin A, Benhamou S
Carcinogenesis30(5)763-8(2009 May)

Crystal structure and RNA binding of the Rpb4/Rpb7 subunits of human RNA polymerase II.
Meka H, Werner F, Cordell SC, Onesti S, Brick P
Nucleic acids research33(19)6435-44(2005)

The Tat/TAR-dependent phosphorylation of RNA polymerase II C-terminal domain stimulates cotranscriptional capping of HIV-1 mRNA.
Zhou M, Deng L, Kashanchi F, Brady JN, Shatkin AJ, Kumar A
Proceedings of the National Academy of Sciences of the United States of America100(22)12666-71(2003 Oct)

Acetylation of Tat defines a cyclinT1-independent step in HIV transactivation.
Kaehlcke K, Dorr A, Hetzer-Egger C, Kiermer V, Henklein P, Schnoelzer M, Loret E, Cole PA, Verdin E, Ott M
Molecular cell12(1)167-76(2003 Jul)

The RNA polymerase II elongation complex.
Shilatifard A, Conaway RC, Conaway JW
Annual review of biochemistry72693-715(2003)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association, gene-gene interaction, and gene-environment interaction. (HuGE Navigator)
Title: Genetic polymorphisms in 85 DNA repair genes and bladder cancer risk.

Identification of the RNA binding region of Rpb4.
Title: Crystal structure and RNA binding of the Rpb4/Rpb7 subunits of human RNA polymerase II.

The following POLR2D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLR2D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04623	NM_004805.4 Latest version!	Homo sapiens RNA polymerase II subunit D (POLR2D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OHu04623	NM_004805.3	Homo sapiens RNA polymerase II subunit D (POLR2D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLR2D ( NM_004805.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004796.1 Homo sapiens RNA polymerase II subunit D (POLR2D), mRNA.

Price & Availability↑

CloneID	OHu04623
Clone ID Related Accession (Same CDS sequence)	NM_004805.3 , NM_004805.4
Accession Version	NM_004805.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerase II subunit RPB4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC093795.1, AC006011.2 and AA775787.1. On Nov 4, 2008 this sequence version replaced NM_004805.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.435605.1, SRR1660807.109582.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2150385 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCGGCGG GTGGCAGCGA TCCGCGGGCT GGCGACGTAG AGGAGGACGC CTCACAGCTC 
ATCTTTCCTA AAGAGTTTGA AACAGCTGAG ACACTTCTAA ATTCAGAAGT TCATATGCTT 
CTGGAACATC GAAAGCAGCA GAATGAGAGT GCAGAGGACG AACAGGAGCT CTCAGAAGTC 
TTCATGAAAA CATTAAACTA CACAGCCCGT TTCAGTCGTT TCAAAAACAG AGAGACCATT 
GCCAGTGTTC GTAGCTTGCT ACTCCAGAAA AAGCTTCATA AGTTTGAGTT GGCCTGTTTG 
GCCAACCTTT GCCCAGAGAC TGCTGAGGAG TCCAAGGCTC TAATCCCAAG CTTGGAGGGA 
CGGTTTGAAG ATGAGGAGCT GCAGCAGATT CTTGATGATA TCCAGACAAA GCGCAGCTTT 
CAGTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004796.1
CDS	56..484
Translation	MAAGGSDPRAGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGRFEDEELQQILDDIQTKRSFQY

Target ORF information:

RefSeq Version	NM_004805.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA polymerase II subunit D (POLR2D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004805.3

ORF Insert Sequence:

ATGGCGGCGG GTGGCAGCGA TCCGCGGGCT GGCGACGTAG AGGAGGACGC CTCACAGCTC 
ATCTTTCCTA AAGAGTTTGA AACAGCTGAG ACACTTCTAA ATTCAGAAGT TCATATGCTT 
CTGGAACATC GAAAGCAGCA GAATGAGAGT GCAGAGGACG AACAGGAGCT CTCAGAAGTC 
TTCATGAAAA CATTAAACTA CACAGCCCGT TTCAGTCGTT TCAAAAACAG AGAGACCATT 
GCCAGTGTTC GTAGCTTGCT ACTCCAGAAA AAGCTTCATA AGTTTGAGTT GGCCTGTTTG 
GCCAACCTTT GCCCAGAGAC TGCTGAGGAG TCCAAGGCTC TAATCCCAAG CTTGGAGGGA 
CGGTTTGAAG ATGAGGAGCT GCAGCAGATT CTTGATGATA TCCAGACAAA GCGCAGCTTT 
CAGTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLR2D ( NM_004805.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004796.1 Homo sapiens RNA polymerase II subunit D (POLR2D), mRNA.

Price & Availability↑

CloneID	OHu04623
Clone ID Related Accession (Same CDS sequence)	NM_004805.3 , NM_004805.4
Accession Version	NM_004805.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerase II subunit RPB4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC093795.1, AC006011.2 and AA775787.1. On Nov 23, 2018 this sequence version replaced NM_004805.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC093795.1, SRR1163655.112702.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2150385 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000272645.9/ ENSP00000272645.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCGG GTGGCAGCGA TCCGCGGGCT GGCGACGTAG AGGAGGACGC CTCACAGCTC 
ATCTTTCCTA AAGAGTTTGA AACAGCTGAG ACACTTCTAA ATTCAGAAGT TCATATGCTT 
CTGGAACATC GAAAGCAGCA GAATGAGAGT GCAGAGGACG AACAGGAGCT CTCAGAAGTC 
TTCATGAAAA CATTAAACTA CACAGCCCGT TTCAGTCGTT TCAAAAACAG AGAGACCATT 
GCCAGTGTTC GTAGCTTGCT ACTCCAGAAA AAGCTTCATA AGTTTGAGTT GGCCTGTTTG 
GCCAACCTTT GCCCAGAGAC TGCTGAGGAG TCCAAGGCTC TAATCCCAAG CTTGGAGGGA 
CGGTTTGAAG ATGAGGAGCT GCAGCAGATT CTTGATGATA TCCAGACAAA GCGCAGCTTT 
CAGTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004796.1
CDS	56..484
Translation	MAAGGSDPRAGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGRFEDEELQQILDDIQTKRSFQY

Target ORF information:

RefSeq Version	NM_004805.4
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA polymerase II subunit D (POLR2D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004805.4

ORF Insert Sequence:

ATGGCGGCGG GTGGCAGCGA TCCGCGGGCT GGCGACGTAG AGGAGGACGC CTCACAGCTC 
ATCTTTCCTA AAGAGTTTGA AACAGCTGAG ACACTTCTAA ATTCAGAAGT TCATATGCTT 
CTGGAACATC GAAAGCAGCA GAATGAGAGT GCAGAGGACG AACAGGAGCT CTCAGAAGTC 
TTCATGAAAA CATTAAACTA CACAGCCCGT TTCAGTCGTT TCAAAAACAG AGAGACCATT 
GCCAGTGTTC GTAGCTTGCT ACTCCAGAAA AAGCTTCATA AGTTTGAGTT GGCCTGTTTG 
GCCAACCTTT GCCCAGAGAC TGCTGAGGAG TCCAAGGCTC TAATCCCAAG CTTGGAGGGA 
CGGTTTGAAG ATGAGGAGCT GCAGCAGATT CTTGATGATA TCCAGACAAA GCGCAGCTTT 
CAGTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic polymorphisms in 85 DNA repair genes and bladder cancer risk.
Carcinogenesis30(5)763-8(2009 May)
Michiels S,Laplanche A,Boulet T,Dessen P,Guillonneau B,M?jean A,Desgrandchamps F,Lathrop M,Sarasin A,Benhamou S

Crystal structure and RNA binding of the Rpb4/Rpb7 subunits of human RNA polymerase II.
Nucleic acids research33(19)6435-44(2005)
Meka H,Werner F,Cordell SC,Onesti S,Brick P

The Tat/TAR-dependent phosphorylation of RNA polymerase II C-terminal domain stimulates cotranscriptional capping of HIV-1 mRNA.
Proceedings of the National Academy of Sciences of the United States of America100(22)12666-71(2003 Oct)
Zhou M,Deng L,Kashanchi F,Brady JN,Shatkin AJ,Kumar A

Acetylation of Tat defines a cyclinT1-independent step in HIV transactivation.
Molecular cell12(1)167-76(2003 Jul)
Kaehlcke K,Dorr A,Hetzer-Egger C,Kiermer V,Henklein P,Schnoelzer M,Loret E,Cole PA,Verdin E,Ott M

The RNA polymerase II elongation complex.
Annual review of biochemistry72693-715(2003)
Shilatifard A,Conaway RC,Conaway JW

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POLR2D cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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