NDUFS4 cDNA ORF clone, Homo sapiens(Human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NDUFS4
Entrez Gene ID	4724
Full Name	NADH:ubiquinone oxidoreductase subunit S4
Synonyms	AQDQ,CI-18,CI-18 kDa,CI-AQDQ
General protein information	Preferred Names NADH:ubiquinone oxidoreductase subunit S4 Names NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Q reductase) NADH-ubiquinone oxidoreductase 18 kDa subunit complex I 18kDa subunit complex I-AQDQ mitochondrial respiratory chain complex I (18-KD subunit)
Gene Type	protein-coding
Organism	Homo sapiens(Human)
Genome	Chromosome: 5 Map Location: 5q11.2
Summary	This gene encodes an nuclear-encoded accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I, or NADH:ubiquinone oxidoreductase). Complex I removes electrons from NADH and passes them to the electron acceptor ubiquinone. Mutations in this gene can cause mitochondrial complex I deficiencies such as Leigh syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015].
Disorder MIM:	602694
Disorder Html:	Leigh syndrome, 256000 (3); Mitochondrial complex I deficiency, 252010 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017009491.1	XP_016864980.1	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial isoform X1
NM_002495.4	NP_002486.1	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial isoform 1 precursor
NM_002495.3	NP_002486.1	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial isoform 1 precursor
NM_001318051.2	NP_001304980.1	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial isoform 2 precursor
NM_001318051.1	NP_001304980.1	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial isoform 2 precursor
NM_002495.2	NP_002486.1	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial precursor

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	lpd-5	NP_491359.2
Oryza sativa (rice)	Os07g0585800	NP_001060126.1
	NDUFS4	XP_001096347.1
Danio rerio (zebrafish)	ndufs4	NP_001018512.1
Pan troglodytes (chimpanzee)	NDUFS4	NP_001065262.1
Rattus norvegicus (Norway rat)	Ndufs4	NP_001020317.1
Arabidopsis thaliana (thale cress)	FRO1	NP_201560.1
Canis lupus familiaris (dog)	NDUFS4	XP_005619385.1
Gallus gallus (chicken)	NDUFS4	XP_424788.4
Xenopus tropicalis (tropical clawed frog)	ndufs4	XP_002941075.1
Homo sapiens (human)	NDUFS4	NP_002486.1
Bos taurus (cattle)	NDUFS4	NP_786994.1
Mus musculus (house mouse)	Ndufs4	NP_035017.2

Gene Ontology
Bibliography

Related articles in PubMed

A novel NDUFS4 frameshift mutation causes Leigh disease in the Hutterite population.
Lamont RE, Beaulieu CL, Bernier FP, Sparkes R, Innes AM, Jackel-Cram C, Ober C, Parboosingh JS, Lemire EG
American journal of medical genetics. Part A173(3)596-600(2017 Mar)

Accessory subunits are integral for assembly and function of human mitochondrial complex I.
Stroud DA, Surgenor EE, Formosa LE, Reljic B, Frazier AE, Dibley MG, Osellame LD, Stait T, Beilharz TH, Thorburn DR, Salim A, Ryan MT
Nature538(7623)123-126(2016 Oct)

Ndufs4 related Leigh syndrome: A case report and review of the literature.
Ortigoza-Escobar JD, Oyarzabal A, Montero R, Artuch R, Jou C, Jim?nez C, Gort L, Briones P, Muchart J, L?pez-Gallardo E, Emperador S, Pesini ER, Montoya J, P?rez B, Rodr?guez-Pombo P, P?rez-Due?as B
Mitochondrion2873-8(2016 May)

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The clinical presentations of five individuals of Hutterite descent with Leigh disease are described herein. An identity-by-descent mapping and candidate gene approach was used to identify a novel homozygous c.393dupA frameshift mutation in the NADH dehydrogenase (ubiquinone) Fe-S protein 4 (NDUFS4) gene.
Title: A novel NDUFS4 frameshift mutation causes Leigh disease in the Hutterite population.

The authors concluded that NDUFS4-related Leigh syndrome is invariably linked to an early onset severe phenotype that results in early death.
Title: Ndufs4 related Leigh syndrome: A case report and review of the literature.

The c.462delA deletion led to a complete lack of NDUFS4 peptide in isolated mitochondria, and this deficiency caused an inefficient mitochondrial complex I assembly and Leigh syndrome symptoms.
Title: Functional characterization of the c.462delA mutation in the NDUFS4 subunit gene of mitochondrial complex I.

Mutations in the NDUFS4 gene and its subunits are associated with the mitochondrial complex I deficiency. (Review)
Title: Cellular and animal models for mitochondrial complex I deficiency: a focus on the NDUFS4 subunit.

Elevated expression of CO I and ND4 were associated with gastric tumorigenesis and tumor dedifferentiation
Title: Altered expression of mitochondrial cytochrome?c oxidase?I and NADH dehydrogenase?4 transcripts associated with gastric tumorigenesis and tumor dedifferentiation.

The following NDUFS4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NDUFS4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19691	NM_002495.4 Latest version!	Homo sapiens NADH:ubiquinone oxidoreductase subunit S4 (NDUFS4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu39596	NM_001318051.2 Latest version!	Homo sapiens NADH:ubiquinone oxidoreductase subunit S4 (NDUFS4), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu59234	XM_017009491.1 Latest version!	Homo sapiens NADH:ubiquinone oxidoreductase subunit S4 (NDUFS4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu19691	NM_002495.3	Homo sapiens NADH:ubiquinone oxidoreductase subunit S4 (NDUFS4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu39596	NM_001318051.1	Homo sapiens NADH:ubiquinone oxidoreductase subunit S4 (NDUFS4), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu19691	NM_002495.2	Homo sapiens NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Q reductase) (NDUFS4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NDUFS4 ( NM_002495.4 ) cDNA ORF clone, Homo sapiens(Human) -> NP_002486.1 Homo sapiens NADH:ubiquinone oxidoreductase subunit S4 (NDUFS4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19691
Clone ID Related Accession (Same CDS sequence)	NM_002495.4 , NM_002495.3 , NM_002495.2
Accession Version	NM_002495.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD692133.1, BU954271.1 and AI001139.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_002495.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189652.228584.1, SRR5189655.5102.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000296684.10/ ENSP00000296684.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCGG TGTCAATGTC AGTGGTACTG AGGCAGACGT TGTGGCGGAG AAGGGCAGTG 
GCTGTAGCTG CCCTTTCCGT TTCCAGGGTT CCGACCAGGT CGTTGAGGAC TTCCACATGG 
AGATTGGCAC AGGACCAGAC TCAAGACACA CAACTCATAA CAGTTGATGA AAAATTGGAT 
ATCACTACTT TAACTGGAGT TCCAGAAGAG CATATAAAAA CTAGAAAAGT CAGGATCTTT 
GTTCCTGCTC GCAATAACAT GCAGTCTGGA GTAAACAACA CAAAGAAATG GAAGATGGAG 
TTTGATACCA GAGAGCGATG GGAAAATCCT TTGATGGGTT GGGCATCAAC GGCTGATCCC 
TTATCCAACA TGGTTCTAAC CTTCAGTACT AAAGAAGATG CAGTTTCCTT TGCAGAAAAA 
AATGGATGGA GCTATGACAT TGAAGAGAGG AAGGTTCCAA AACCCAAGTC CAAGTCTTAT 
GGTGCAAACT TTTCTTGGAA CAAAAGAACA AGAGTATCCA CAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002486.1
CDS	25..552
Translation	MAAVSMSVVLRQTLWRRRAVAVAALSVSRVPTRSLRTSTWRLAQDQTQDTQLITVDEKLDITTLTGVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKMEFDTRERWENPLMGWASTADPLSNMVLTFSTKEDAVSFAEKNGWSYDIEERKVPKPKSKSYGANFSWNKRTRVSTK

Target ORF information:

RefSeq Version	NM_002495.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NADH:ubiquinone oxidoreductase subunit S4 (NDUFS4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002495.4

ORF Insert Sequence:

ATGGCGGCGG TGTCAATGTC AGTGGTACTG AGGCAGACGT TGTGGCGGAG AAGGGCAGTG 
GCTGTAGCTG CCCTTTCCGT TTCCAGGGTT CCGACCAGGT CGTTGAGGAC TTCCACATGG 
AGATTGGCAC AGGACCAGAC TCAAGACACA CAACTCATAA CAGTTGATGA AAAATTGGAT 
ATCACTACTT TAACTGGAGT TCCAGAAGAG CATATAAAAA CTAGAAAAGT CAGGATCTTT 
GTTCCTGCTC GCAATAACAT GCAGTCTGGA GTAAACAACA CAAAGAAATG GAAGATGGAG 
TTTGATACCA GAGAGCGATG GGAAAATCCT TTGATGGGTT GGGCATCAAC GGCTGATCCC 
TTATCCAACA TGGTTCTAAC CTTCAGTACT AAAGAAGATG CAGTTTCCTT TGCAGAAAAA 
AATGGATGGA GCTATGACAT TGAAGAGAGG AAGGTTCCAA AACCCAAGTC CAAGTCTTAT 
GGTGCAAACT TTTCTTGGAA CAAAAGAACA AGAGTATCCA CAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFS4 ( NM_002495.3 ) cDNA ORF clone, Homo sapiens(Human) -> NP_002486.1 Homo sapiens NADH:ubiquinone oxidoreductase subunit S4 (NDUFS4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19691
Clone ID Related Accession (Same CDS sequence)	NM_002495.4 , NM_002495.3 , NM_002495.2
Accession Version	NM_002495.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-23
Organism	Homo sapiens(human)
Product	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD692133.1, BU954271.1 and AI001139.1. This sequence is a reference standard in the RefSeqGene project. On Dec 22, 2015 this sequence version replaced NM_002495.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CD173665.1, BI667561.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG TGTCAATGTC AGTGGTACTG AGGCAGACGT TGTGGCGGAG AAGGGCAGTG 
GCTGTAGCTG CCCTTTCCGT TTCCAGGGTT CCGACCAGGT CGTTGAGGAC TTCCACATGG 
AGATTGGCAC AGGACCAGAC TCAAGACACA CAACTCATAA CAGTTGATGA AAAATTGGAT 
ATCACTACTT TAACTGGAGT TCCAGAAGAG CATATAAAAA CTAGAAAAGT CAGGATCTTT 
GTTCCTGCTC GCAATAACAT GCAGTCTGGA GTAAACAACA CAAAGAAATG GAAGATGGAG 
TTTGATACCA GAGAGCGATG GGAAAATCCT TTGATGGGTT GGGCATCAAC GGCTGATCCC 
TTATCCAACA TGGTTCTAAC CTTCAGTACT AAAGAAGATG CAGTTTCCTT TGCAGAAAAA 
AATGGATGGA GCTATGACAT TGAAGAGAGG AAGGTTCCAA AACCCAAGTC CAAGTCTTAT 
GGTGCAAACT TTTCTTGGAA CAAAAGAACA AGAGTATCCA CAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002486.1
CDS	31..558
Translation	MAAVSMSVVLRQTLWRRRAVAVAALSVSRVPTRSLRTSTWRLAQDQTQDTQLITVDEKLDITTLTGVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKMEFDTRERWENPLMGWASTADPLSNMVLTFSTKEDAVSFAEKNGWSYDIEERKVPKPKSKSYGANFSWNKRTRVSTK

Target ORF information:

RefSeq Version	NM_002495.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NADH:ubiquinone oxidoreductase subunit S4 (NDUFS4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002495.3

ORF Insert Sequence:

ATGGCGGCGG TGTCAATGTC AGTGGTACTG AGGCAGACGT TGTGGCGGAG AAGGGCAGTG 
GCTGTAGCTG CCCTTTCCGT TTCCAGGGTT CCGACCAGGT CGTTGAGGAC TTCCACATGG 
AGATTGGCAC AGGACCAGAC TCAAGACACA CAACTCATAA CAGTTGATGA AAAATTGGAT 
ATCACTACTT TAACTGGAGT TCCAGAAGAG CATATAAAAA CTAGAAAAGT CAGGATCTTT 
GTTCCTGCTC GCAATAACAT GCAGTCTGGA GTAAACAACA CAAAGAAATG GAAGATGGAG 
TTTGATACCA GAGAGCGATG GGAAAATCCT TTGATGGGTT GGGCATCAAC GGCTGATCCC 
TTATCCAACA TGGTTCTAAC CTTCAGTACT AAAGAAGATG CAGTTTCCTT TGCAGAAAAA 
AATGGATGGA GCTATGACAT TGAAGAGAGG AAGGTTCCAA AACCCAAGTC CAAGTCTTAT 
GGTGCAAACT TTTCTTGGAA CAAAAGAACA AGAGTATCCA CAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFS4 ( NM_002495.2 ) cDNA ORF clone, Homo sapiens(Human) -> NP_002486.1 Homo sapiens NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Q reductase) (NDUFS4), mRNA.

Price & Availability↑

CloneID	OHu19691
Clone ID Related Accession (Same CDS sequence)	NM_002495.4 , NM_002495.3 , NM_002495.2
Accession Version	NM_002495.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial precursor
Comment

ATGGCGGCGG TGTCAATGTC AGTGGTACTG AGGCAGACGT TGTGGCGGAG AAGGGCAGTG 
GCTGTAGCTG CCCTTTCCGT TTCCAGGGTT CCGACCAGGT CGTTGAGGAC TTCCACATGG 
AGATTGGCAC AGGACCAGAC TCAAGACACA CAACTCATAA CAGTTGATGA AAAATTGGAT 
ATCACTACTT TAACTGGAGT TCCAGAAGAG CATATAAAAA CTAGAAAAGT CAGGATCTTT 
GTTCCTGCTC GCAATAACAT GCAGTCTGGA GTAAACAACA CAAAGAAATG GAAGATGGAG 
TTTGATACCA GAGAGCGATG GGAAAATCCT TTGATGGGTT GGGCATCAAC GGCTGATCCC 
TTATCCAACA TGGTTCTAAC CTTCAGTACT AAAGAAGATG CAGTTTCCTT TGCAGAAAAA 
AATGGATGGA GCTATGACAT TGAAGAGAGG AAGGTTCCAA AACCCAAGTC CAAGTCTTAT 
GGTGCAAACT TTTCTTGGAA CAAAAGAACA AGAGTATCCA CAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002486.1
Translation	MAAVSMSVVLRQTLWRRRAVAVAALSVSRVPTRSLRTSTWRLAQDQTQDTQLITVDEKLDITTLTGVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKMEFDTRERWENPLMGWASTADPLSNMVLTFSTKEDAVSFAEKNGWSYDIEERKVPKPKSKSYGANFSWNKRTRVSTK

Target ORF information:

RefSeq Version	NM_002495.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Q reductase) (NDUFS4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002495.2

ORF Insert Sequence:

ATGGCGGCGG TGTCAATGTC AGTGGTACTG AGGCAGACGT TGTGGCGGAG AAGGGCAGTG 
GCTGTAGCTG CCCTTTCCGT TTCCAGGGTT CCGACCAGGT CGTTGAGGAC TTCCACATGG 
AGATTGGCAC AGGACCAGAC TCAAGACACA CAACTCATAA CAGTTGATGA AAAATTGGAT 
ATCACTACTT TAACTGGAGT TCCAGAAGAG CATATAAAAA CTAGAAAAGT CAGGATCTTT 
GTTCCTGCTC GCAATAACAT GCAGTCTGGA GTAAACAACA CAAAGAAATG GAAGATGGAG 
TTTGATACCA GAGAGCGATG GGAAAATCCT TTGATGGGTT GGGCATCAAC GGCTGATCCC 
TTATCCAACA TGGTTCTAAC CTTCAGTACT AAAGAAGATG CAGTTTCCTT TGCAGAAAAA 
AATGGATGGA GCTATGACAT TGAAGAGAGG AAGGTTCCAA AACCCAAGTC CAAGTCTTAT 
GGTGCAAACT TTTCTTGGAA CAAAAGAACA AGAGTATCCA CAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFS4 ( NM_001318051.2 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001304980.1 Homo sapiens NADH:ubiquinone oxidoreductase subunit S4 (NDUFS4), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu39596
Clone ID Related Accession (Same CDS sequence)	NM_001318051.2 , NM_001318051.1
Accession Version	NM_001318051.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD692133.1, BU145342.1 and BU954271.1. On May 31, 2019 this sequence version replaced NM_001318051.1. Transcript Variant: This variant (5) lacks an alternate exon, which results in a frameshift compared to variant 1. The encoded isoform (2) has a distinct, shorter C-terminus than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU145342.1, SRR1163658.194438.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG TGTCAATGTC AGTGGTACTG AGGCAGACGT TGTGGCGGAG AAGGGCAGTG 
GCTGTAGCTG CCCTTTCCGT TTCCAGGGTT CCGACCAGGT CGTTGAGGAC TTCCACATGG 
AGATTGGCAC AGGACCAGAC TCAAGACACA CAACTCATAA CAGTTGATGA AAAATTGGAT 
ATCACTACTT TAACTGGAGT TCCAGAAGAG CATATAAAAA CTAGAAAAGT CAGGATCTTT 
GTTCCTGCTC GCAATAACAT GCAGTCTGGA GTAAACAACA CAAAGAAATG GAAGATGGAG 
TTTGATACCA GAGAGCGATG GGAAAATCCT TTGATGGGTT GGGCATCAAC GATGGAGCTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304980.1
CDS	25..387
Translation	MAAVSMSVVLRQTLWRRRAVAVAALSVSRVPTRSLRTSTWRLAQDQTQDTQLITVDEKLDITTLTGVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKMEFDTRERWENPLMGWASTMEL

Target ORF information:

RefSeq Version	NM_001318051.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NADH:ubiquinone oxidoreductase subunit S4 (NDUFS4), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318051.2

ORF Insert Sequence:

ATGGCGGCGG TGTCAATGTC AGTGGTACTG AGGCAGACGT TGTGGCGGAG AAGGGCAGTG 
GCTGTAGCTG CCCTTTCCGT TTCCAGGGTT CCGACCAGGT CGTTGAGGAC TTCCACATGG 
AGATTGGCAC AGGACCAGAC TCAAGACACA CAACTCATAA CAGTTGATGA AAAATTGGAT 
ATCACTACTT TAACTGGAGT TCCAGAAGAG CATATAAAAA CTAGAAAAGT CAGGATCTTT 
GTTCCTGCTC GCAATAACAT GCAGTCTGGA GTAAACAACA CAAAGAAATG GAAGATGGAG 
TTTGATACCA GAGAGCGATG GGAAAATCCT TTGATGGGTT GGGCATCAAC GATGGAGCTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFS4 ( NM_001318051.1 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001304980.1 Homo sapiens NADH:ubiquinone oxidoreductase subunit S4 (NDUFS4), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu39596
Clone ID Related Accession (Same CDS sequence)	NM_001318051.2 , NM_001318051.1
Accession Version	NM_001318051.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD692133.1, BU145342.1, BU954271.1 and AI001139.1. On Dec 22, 2015 this sequence version replaced XM_005248525.3. Transcript Variant: This variant (5) lacks an alternate exon, which results in a frameshift compared to variant 1. The encoded isoform (2) has a distinct, shorter C-terminus than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.447168.1, BU145342.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG TGTCAATGTC AGTGGTACTG AGGCAGACGT TGTGGCGGAG AAGGGCAGTG 
GCTGTAGCTG CCCTTTCCGT TTCCAGGGTT CCGACCAGGT CGTTGAGGAC TTCCACATGG 
AGATTGGCAC AGGACCAGAC TCAAGACACA CAACTCATAA CAGTTGATGA AAAATTGGAT 
ATCACTACTT TAACTGGAGT TCCAGAAGAG CATATAAAAA CTAGAAAAGT CAGGATCTTT 
GTTCCTGCTC GCAATAACAT GCAGTCTGGA GTAAACAACA CAAAGAAATG GAAGATGGAG 
TTTGATACCA GAGAGCGATG GGAAAATCCT TTGATGGGTT GGGCATCAAC GATGGAGCTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304980.1
CDS	31..393
Translation	MAAVSMSVVLRQTLWRRRAVAVAALSVSRVPTRSLRTSTWRLAQDQTQDTQLITVDEKLDITTLTGVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKMEFDTRERWENPLMGWASTMEL

Target ORF information:

RefSeq Version	NM_001318051.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NADH:ubiquinone oxidoreductase subunit S4 (NDUFS4), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318051.1

ORF Insert Sequence:

ATGGCGGCGG TGTCAATGTC AGTGGTACTG AGGCAGACGT TGTGGCGGAG AAGGGCAGTG 
GCTGTAGCTG CCCTTTCCGT TTCCAGGGTT CCGACCAGGT CGTTGAGGAC TTCCACATGG 
AGATTGGCAC AGGACCAGAC TCAAGACACA CAACTCATAA CAGTTGATGA AAAATTGGAT 
ATCACTACTT TAACTGGAGT TCCAGAAGAG CATATAAAAA CTAGAAAAGT CAGGATCTTT 
GTTCCTGCTC GCAATAACAT GCAGTCTGGA GTAAACAACA CAAAGAAATG GAAGATGGAG 
TTTGATACCA GAGAGCGATG GGAAAATCCT TTGATGGGTT GGGCATCAAC GATGGAGCTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFS4 ( XM_017009491.1 ) cDNA ORF clone, Homo sapiens(Human) -> XP_016864980.1 Homo sapiens NADH:ubiquinone oxidoreductase subunit S4 (NDUFS4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu59234
Clone ID Related Accession (Same CDS sequence)	XM_017009491.1
Accession Version	XM_017009491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG TGTCAATGTC AGTGGTACTG AGGCAGACGT TGTGGCGGAG AAGGGCAGTG 
GCTGTAGCTG CCCTTTCCGT TTCCAGGGTT CCGACCAGGT CGTTGAGGAC TTCCACATGG 
AGATTGGCAC AGGACCAGAC TCAAGACACA CAACTCATAA CAGTTGATGA AAAATTGGTT 
TATCTTGTAC CACTGTGTTT TACATTCTGT GGTCTAGCCA CACCAAGCCT GGAACTTGCT 
ACTCTTTTTC CTGCTTCAAG GCATTTGGAG TTCCTGTTCA CTCTACCTGG ATGCATTTTA 
ACTAATCCTG AGGACTTCAT CCGGTCACCA TCTACCTATT CTTCAAATGC CACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864980.1
CDS	54..410
Translation	MAAVSMSVVLRQTLWRRRAVAVAALSVSRVPTRSLRTSTWRLAQDQTQDTQLITVDEKLVYLVPLCFTFCGLATPSLELATLFPASRHLEFLFTLPGCILTNPEDFIRSPSTYSSNAT

Target ORF information:

RefSeq Version	XM_017009491.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NADH:ubiquinone oxidoreductase subunit S4 (NDUFS4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009491.1

ORF Insert Sequence:

ATGGCGGCGG TGTCAATGTC AGTGGTACTG AGGCAGACGT TGTGGCGGAG AAGGGCAGTG 
GCTGTAGCTG CCCTTTCCGT TTCCAGGGTT CCGACCAGGT CGTTGAGGAC TTCCACATGG 
AGATTGGCAC AGGACCAGAC TCAAGACACA CAACTCATAA CAGTTGATGA AAAATTGGTT 
TATCTTGTAC CACTGTGTTT TACATTCTGT GGTCTAGCCA CACCAAGCCT GGAACTTGCT 
ACTCTTTTTC CTGCTTCAAG GCATTTGGAG TTCCTGTTCA CTCTACCTGG ATGCATTTTA 
ACTAATCCTG AGGACTTCAT CCGGTCACCA TCTACCTATT CTTCAAATGC CACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel NDUFS4 frameshift mutation causes Leigh disease in the Hutterite population.
American journal of medical genetics. Part A173(3)596-600(2017 Mar)
Lamont RE,Beaulieu CL,Bernier FP,Sparkes R,Innes AM,Jackel-Cram C,Ober C,Parboosingh JS,Lemire EG

Accessory subunits are integral for assembly and function of human mitochondrial complex I.
Nature538(7623)123-126(2016 Oct)
Stroud DA,Surgenor EE,Formosa LE,Reljic B,Frazier AE,Dibley MG,Osellame LD,Stait T,Beilharz TH,Thorburn DR,Salim A,Ryan MT

Ndufs4 related Leigh syndrome: A case report and review of the literature.
Mitochondrion2873-8(2016 May)
Ortigoza-Escobar JD,Oyarzabal A,Montero R,Artuch R,Jou C,Jim?nez C,Gort L,Briones P,Muchart J,L?pez-Gallardo E,Emperador S,Pesini ER,Montoya J,P?rez B,Rodr?guez-Pombo P,P?rez-Due?as B

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

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NDUFS4 cDNA ORF clone, Homo sapiens(Human)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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