TYW1B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TYW1B
Entrez Gene ID	441250
Full Name	tRNA-yW synthesizing protein 1 homolog B
Synonyms	LINC00069,NCRNA00069,RSAFD2
General protein information	Preferred Names tRNA-yW synthesizing protein 1 homolog B Names S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase long intergenic non-protein coding RNA 69 radical S-adenosyl methionine and flavodoxin domain-containing protein 2 radical S-adenosyl methionine and flavodoxin domains 1 tRNA wybutosine-synthesizing protein 1 homolog B tRNA-yW synthesizing protein 1 homolog B (non-protein coding)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q11.22-q11.23
Summary	Wybutosine is a hypermodified guanosine found in phenylalanine tRNA. Wybutosine functions to stabilize codon-anticodon interactions during ribosome decoding and therefore supports the maintenance of the reading frame. In yeast, the homolog of this gene is essential for the synthesis of wybutosine. The human genome contains two closely related genes that putatively function in wybutosine synthesis. The open reading frame of this locus is disrupted in some individuals. Thus, this locus appears to be an evolving pseudogene, but may still be functional in some members of the population. [provided by RefSeq, Apr 2014].

mRNA and Protein(s)

mRNA	Protein	Name
XM_011516231.2	XP_011514533.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X3
XM_011516232.3	XP_011514534.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X4
NM_001145440.2	NP_001138912.2	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
XM_011516230.2	XP_011514532.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X1
XM_017012221.1	XP_016867710.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X2
XM_011516233.2	XP_011514535.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X5
XM_017012222.1	XP_016867711.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X6
XM_011516230.3	XP_011514532.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X1
XM_017012222.2	XP_016867711.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X6
XM_011516232.2	XP_011514534.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X4
NM_001145440.3	NP_001138912.2	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1B

Pathways from BioSystems

Homologies

Homo sapiens (human)	TYW1B	NP_001138912.1
Pan troglodytes (chimpanzee)	TYW1B	XP_003954562.1

Gene Ontology
Bibliography

Related articles in PubMed

Discovery and refinement of loci associated with lipid levels.
Willer CJ, Schmidt EM, Sengupta S, Peloso GM, Gustafsson S, Kanoni S, Ganna A, Chen J, Buchkovich ML, Mora S, Beckmann JS, Bragg-Gresham JL, Chang HY, Demirkan A, Den Hertog HM, Do R, Donnelly LA, Ehret GB, Esko T, Feitosa MF, Ferreira T, Fischer K, Fontanillas P, Fraser RM, Freitag DF, Gurdasani D, Heikkil? K, Hypp?nen E, Isaacs A, Jackson AU, Johansson ?, Johnson T, Kaakinen M, Kettunen J, Kleber ME, Li X, Luan J, Lyytik?inen LP, Magnusson PKE, Mangino M, Mihailov E, Montasser ME, M?ller-Nurasyid M, Nolte IM, O'Connell JR, Palmer CD, Perola M, Petersen AK, Sanna S, Saxena R, Service SK, Shah S, Shungin D, Sidore C, Song C, Strawbridge RJ, Surakka I, Tanaka T, Teslovich TM, Thorleifsson G, Van den Herik EG, Voight BF, Volcik KA, Waite LL, Wong A, Wu Y, Zhang W, Absher D, Asiki G, Barroso I, Been LF, Bolton JL, Bonnycastle LL, Brambilla P, Burnett MS, Cesana G, Dimitriou M, Doney ASF, D?ring A, Elliott P, Epstein SE, Ingi Eyjolfsson G, Gigante B, Goodarzi MO, Grallert H, Gravito ML, Groves CJ, Hallmans G, Hartikainen AL, Hayward C, Hernandez D, Hicks AA, Holm H, Hung YJ, Illig T, Jones MR, Kaleebu P, Kastelein JJP, Khaw KT, Kim E, Klopp N, Komulainen P, Kumari M, Langenberg C, Lehtim?ki T, Lin SY, Lindstr?m J, Loos RJF, Mach F, McArdle WL, Meisinger C, Mitchell BD, M?ller G, Nagaraja R, Narisu N, Nieminen TVM, Nsubuga RN, Olafsson I, Ong KK, Palotie A, Papamarkou T, Pomilla C, Pouta A, Rader DJ, Reilly MP, Ridker PM, Rivadeneira F, Rudan I, Ruokonen A, Samani N, Scharnagl H, Seeley J, Silander K, Stan??kov? A, Stirrups K, Swift AJ, Tiret L, Uitterlinden AG, van Pelt LJ, Vedantam S, Wainwright N, Wijmenga C, Wild SH, Willemsen G, Wilsgaard T, Wilson JF, Young EH, Zhao JH, Adair LS, Arveiler D, Assimes TL, Bandinelli S, Bennett F, Bochud M, Boehm BO, Boomsma DI, Borecki IB, Bornstein SR, Bovet P, Burnier M, Campbell H, Chakravarti A, Chambers JC, Chen YI, Collins FS, Cooper RS, Danesh J, Dedoussis G, de Faire U, Feranil AB, Ferri?res J, Ferrucci L, Freimer NB, Gieger C, Groop LC, Gudnason V, Gyllensten U, Hamsten A, Harris TB, Hingorani A, Hirschhorn JN, Hofman A, Hovingh GK, Hsiung CA, Humphries SE, Hunt SC, Hveem K, Iribarren C, J?rvelin MR, Jula A, K?h?nen M, Kaprio J, Kes?niemi A, Kivimaki M, Kooner JS, Koudstaal PJ, Krauss RM, Kuh D, Kuusisto J, Kyvik KO, Laakso M, Lakka TA, Lind L, Lindgren CM, Martin NG, M?rz W, McCarthy MI, McKenzie CA, Meneton P, Metspalu A, Moilanen L, Morris AD, Munroe PB, Nj?lstad I, Pedersen NL, Power C, Pramstaller PP, Price JF, Psaty BM, Quertermous T, Rauramaa R, Saleheen D, Salomaa V, Sanghera DK, Saramies J, Schwarz PEH, Sheu WH, Shuldiner AR, Siegbahn A, Spector TD, Stefansson K, Strachan DP, Tayo BO, Tremoli E, Tuomilehto J, Uusitupa M, van Duijn CM, Vollenweider P, Wallentin L, Wareham NJ, Whitfield JB, Wolffenbuttel BHR, Ordovas JM, Boerwinkle E, Palmer CNA, Thorsteinsdottir U, Chasman DI, Rotter JI, Franks PW, Ripatti S, Cupples LA, Sandhu MS, Rich SS, Boehnke M, Deloukas P, Kathiresan S, Mohlke KL, Ingelsson E, Abecasis GR,
Nature genetics45(11)1274-1283(2013 Nov)

Biological, clinical and population relevance of 95 loci for blood lipids.
Teslovich TM, Musunuru K, Smith AV, Edmondson AC, Stylianou IM, Koseki M, Pirruccello JP, Ripatti S, Chasman DI, Willer CJ, Johansen CT, Fouchier SW, Isaacs A, Peloso GM, Barbalic M, Ricketts SL, Bis JC, Aulchenko YS, Thorleifsson G, Feitosa MF, Chambers J, Orho-Melander M, Melander O, Johnson T, Li X, Guo X, Li M, Shin Cho Y, Jin Go M, Jin Kim Y, Lee JY, Park T, Kim K, Sim X, Twee-Hee Ong R, Croteau-Chonka DC, Lange LA, Smith JD, Song K, Hua Zhao J, Yuan X, Luan J, Lamina C, Ziegler A, Zhang W, Zee RY, Wright AF, Witteman JC, Wilson JF, Willemsen G, Wichmann HE, Whitfield JB, Waterworth DM, Wareham NJ, Waeber G, Vollenweider P, Voight BF, Vitart V, Uitterlinden AG, Uda M, Tuomilehto J, Thompson JR, Tanaka T, Surakka I, Stringham HM, Spector TD, Soranzo N, Smit JH, Sinisalo J, Silander K, Sijbrands EJ, Scuteri A, Scott J, Schlessinger D, Sanna S, Salomaa V, Saharinen J, Sabatti C, Ruokonen A, Rudan I, Rose LM, Roberts R, Rieder M, Psaty BM, Pramstaller PP, Pichler I, Perola M, Penninx BW, Pedersen NL, Pattaro C, Parker AN, Pare G, Oostra BA, O'Donnell CJ, Nieminen MS, Nickerson DA, Montgomery GW, Meitinger T, McPherson R, McCarthy MI, McArdle W, Masson D, Martin NG, Marroni F, Mangino M, Magnusson PK, Lucas G, Luben R, Loos RJ, Lokki ML, Lettre G, Langenberg C, Launer LJ, Lakatta EG, Laaksonen R, Kyvik KO, Kronenberg F, K?nig IR, Khaw KT, Kaprio J, Kaplan LM, Johansson A, Jarvelin MR, Janssens AC, Ingelsson E, Igl W, Kees Hovingh G, Hottenga JJ, Hofman A, Hicks AA, Hengstenberg C, Heid IM, Hayward C, Havulinna AS, Hastie ND, Harris TB, Haritunians T, Hall AS, Gyllensten U, Guiducci C, Groop LC, Gonzalez E, Gieger C, Freimer NB, Ferrucci L, Erdmann J, Elliott P, Ejebe KG, D?ring A, Dominiczak AF, Demissie S, Deloukas P, de Geus EJ, de Faire U, Crawford G, Collins FS, Chen YD, Caulfield MJ, Campbell H, Burtt NP, Bonnycastle LL, Boomsma DI, Boekholdt SM, Bergman RN, Barroso I, Bandinelli S, Ballantyne CM, Assimes TL, Quertermous T, Altshuler D, Seielstad M, Wong TY, Tai ES, Feranil AB, Kuzawa CW, Adair LS, Taylor HA Jr, Borecki IB, Gabriel SB, Wilson JG, Holm H, Thorsteinsdottir U, Gudnason V, Krauss RM, Mohlke KL, Ordovas JM, Munroe PB, Kooner JS, Tall AR, Hegele RA, Kastelein JJ, Schadt EE, Rotter JI, Boerwinkle E, Strachan DP, Mooser V, Stefansson K, Reilly MP, Samani NJ, Schunkert H, Cupples LA, Sandhu MS, Ridker PM, Rader DJ, van Duijn CM, Peltonen L, Abecasis GR, Boehnke M, Kathiresan S
Nature466(7307)707-13(2010 Aug)

Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis.
Noma A, Suzuki T
Nucleic acids symposium series (2004)(50)65-6(2006)

Discovery of a gene family critical to wyosine base formation in a subset of phenylalanine-specific transfer RNAs.
Waas WF, de Cr?cy-Lagard V, Schimmel P
The Journal of biological chemistry280(45)37616-22(2005 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

The following TYW1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TYW1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25170	NM_001145440.3 Latest version!	Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu80327	XM_011516230.3 Latest version!	Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu80328	XM_011516231.2 Latest version!	Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu80329	XM_011516232.3 Latest version!	Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu80330	XM_011516233.2 Latest version!	Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu97262	XM_017012222.2 Latest version!	Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu97261	XM_017012221.1 Latest version!	Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu80327	XM_011516230.2	Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu97262	XM_017012222.1	Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu80329	XM_011516232.2	Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu25170	NM_001145440.2	Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TYW1B ( NM_001145440.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001138912.2 Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), mRNA.

Price & Availability↑

CloneID	OHu25170
Clone ID Related Accession (Same CDS sequence)	NM_001145440.2 , NM_001145440.3
Accession Version	NM_001145440.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-10
Organism	Homo sapiens(human)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA769764.1, BC068520.1, BC106019.1 and AI984113.1. On Nov 22, 2018 this sequence version replaced NM_001145440.2. ##Evidence-Data-START## Transcript exon combination :: BC068520.1, SRR1660803.235670.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000620995.5/ ENSP00000482502.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGATCCTT CTGCGGATAC ATGGGACCTC TCCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATCTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCGAGA TGCAGGGATT TGCAACAGTT CTTGCTGAAG CAGTTACGTC CCTGGATCTG 
CCTGTGGCCA TTATTAATCT AAAAGAATAT GATCCAGATG ATCATCTGAT AGAAGAGGTG 
ACTAGTAAAA ATGTCTGTGT CTTCCTGGTT GCGACATACA CTGACGGCCT ACCAACCGAA 
AGTGCAGAGT GGTTCTGCAA ATGGTTAGAG GAAGCATCCA TTGATTTTCG ATTTGGCAAA 
ACTTACCTGA AGGGTATGAG AGATGCGGTA TTTGGCCTGG GAAATTCTGC CTATGCTAGC 
CACTTCAACA AGGTTGGCAA AAATGTTGAC AAGTGGCTCT GGATGCTTGG CGTGCATCGT 
GTGATGAGTC GAGGGGAGGG CGACTGCGAC GTGGTTAAAA GCAAGCACGG CAGCATTGAG 
GCCAACTTCA GAGCATGGAA GACCAAGTTC ATCTCCCAGC TGCAGGCACT TCAGAAAGGG 
GAGAGAAAGA AGTCCTGTGG CGGCCACTGC AAGAAAGGCA AATGTGAATC TCACCAACAT 
GGCTCAGAGG AGAGGGAGGA AGGATCTCAA GAGCAGGACG AATTGCATCA CAGAGACACC 
AAGGAGGAAG AACCCTTCGA GAGCTCCAGT GAAGAAGAGT TTGGTGGTGA GGACCATCAG 
AGCCTAAATT CCATTGTTGA TGTTGAAGAT TTGGGCAAAA TTATGGATCA CGTGAAGAAA 
GAAAAGAGAG AAAAGGAACA GCAGGAAGAG AAGTCTGGTT TGTTCAGGAA CATGGGGAGG 
AATGAAGATG GTGAAAGAAG AGCTATGATA ACTCCTGCTC TCCGAGAAGC CCTTACTAAA 
CAAGTCGATG CTCCGAGGGA GAGGAGCTTG TTACAAACAC ACATTCTATG GAATGAGAGC 
CATCGCTGCA TGGAAACCAC CCCGAGCTTG GCGTGTGCTA ATAAATGTGT CTTCTGTTGG 
TGGCACCACA ACAACCCTGT GGGCACTGAA TGGTTGTGGA AGATGGACCA GCCTGAAATG 
ATCTTGAAGG AAGCCATTGA AAACCATCAG AACATGATTA AGCAGTTTAA AGGAGTACCG 
GGCGTCAAAG CAGAACGCTT TGAAGAAGGA ATGACGGTAA AGCACTGTGC ATTGTCCCTC 
GTGGGAGAAC CAATAATGTA CCCAGAGATC AACAGGTTTT TGAAGCTACT CCACCAGTGT 
AAAATCTCCA GCTTCCTGGT CACAAATGCA CAATTTCCTG CGGAAATCAG GAACCTCGAG 
CCAGTTACTC AGCTGTATGT CAGTGTGGAT GCCAGTACCA AAGACAGCCT GAAGAAAATC 
GACCGCCCAC TCTTCAAGGA TTTCTGGCAG CAATTCCTTG ACAGTTTAAA AGCCTTGGCA 
GTCAAGCAAC AACGAACTGT CTACAGACTG ATGCTCGTGA AAGCATGGAA CGTGGACGAG 
CTCCAGGCCT ACGCGCAGCT CGTGTCCCTG GGGAATCCTG ACTTCATCGA AGTGAAGGGC 
GTTACCTACT GCAGAGAAAG TTCAGCAAGC AGTCTTACCA TGGCCCATGT GCCCTGGCAT 
GAGGAAGTGG TACAGTTTGT CCGCGAGCTG GTGGATCTGA TCCCCGAATA TGAAATTGCA 
TGTGAACACG AACACTCTAA TTGCCTCCTG ATAGCACACA GAAAGTTTAA AATTGGTGGT 
GAATGGTGGA CATGGATCGA TTATAACCGC TTCCAGGAGC TCATCCAGGA ATATGAAGAT 
AGTGGTGGAT CAAAAACGTT CAGCGCAAAG GATTATATGG CCAGAACTCC TCACTGGGCA 
TTATTTGGTG CCAATGAAAG AAGCTTTGAT CCCAAGGACA CAAGACATCA GAGAAAGAAC 
AAATCAAAGG CTATTTCTGG ATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138912.2
CDS	126..2132
Translation	MDPSADTWDLSSPLISLWINRFYIYLGFAVSISLWICVQIVIEMQGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRDAVFGLGNSAYASHFNKVGKNVDKWLWMLGVHRVMSRGEGDCDVVKSKHGSIEANFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSQEQDELHHRDTKEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQVDAPRERSLLQTHILWNESHRCMETTPSLACANKCVFCWWHHNNPVGTEWLWKMDQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQQFLDSLKALAVKQQRTVYRLMLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCRESSASSLTMAHVPWHEEVVQFVRELVDLIPEYEIACEHEHSNCLLIAHRKFKIGGEWWTWIDYNRFQELIQEYEDSGGSKTFSAKDYMARTPHWALFGANERSFDPKDTRHQRKNKSKAISGC

Target ORF information:

RefSeq Version	NM_001145440.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145440.3

ORF Insert Sequence:

ATGGATCCTT CTGCGGATAC ATGGGACCTC TCCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATCTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCGAGA TGCAGGGATT TGCAACAGTT CTTGCTGAAG CAGTTACGTC CCTGGATCTG 
CCTGTGGCCA TTATTAATCT AAAAGAATAT GATCCAGATG ATCATCTGAT AGAAGAGGTG 
ACTAGTAAAA ATGTCTGTGT CTTCCTGGTT GCGACATACA CTGACGGCCT ACCAACCGAA 
AGTGCAGAGT GGTTCTGCAA ATGGTTAGAG GAAGCATCCA TTGATTTTCG ATTTGGCAAA 
ACTTACCTGA AGGGTATGAG AGATGCGGTA TTTGGCCTGG GAAATTCTGC CTATGCTAGC 
CACTTCAACA AGGTTGGCAA AAATGTTGAC AAGTGGCTCT GGATGCTTGG CGTGCATCGT 
GTGATGAGTC GAGGGGAGGG CGACTGCGAC GTGGTTAAAA GCAAGCACGG CAGCATTGAG 
GCCAACTTCA GAGCATGGAA GACCAAGTTC ATCTCCCAGC TGCAGGCACT TCAGAAAGGG 
GAGAGAAAGA AGTCCTGTGG CGGCCACTGC AAGAAAGGCA AATGTGAATC TCACCAACAT 
GGCTCAGAGG AGAGGGAGGA AGGATCTCAA GAGCAGGACG AATTGCATCA CAGAGACACC 
AAGGAGGAAG AACCCTTCGA GAGCTCCAGT GAAGAAGAGT TTGGTGGTGA GGACCATCAG 
AGCCTAAATT CCATTGTTGA TGTTGAAGAT TTGGGCAAAA TTATGGATCA CGTGAAGAAA 
GAAAAGAGAG AAAAGGAACA GCAGGAAGAG AAGTCTGGTT TGTTCAGGAA CATGGGGAGG 
AATGAAGATG GTGAAAGAAG AGCTATGATA ACTCCTGCTC TCCGAGAAGC CCTTACTAAA 
CAAGTCGATG CTCCGAGGGA GAGGAGCTTG TTACAAACAC ACATTCTATG GAATGAGAGC 
CATCGCTGCA TGGAAACCAC CCCGAGCTTG GCGTGTGCTA ATAAATGTGT CTTCTGTTGG 
TGGCACCACA ACAACCCTGT GGGCACTGAA TGGTTGTGGA AGATGGACCA GCCTGAAATG 
ATCTTGAAGG AAGCCATTGA AAACCATCAG AACATGATTA AGCAGTTTAA AGGAGTACCG 
GGCGTCAAAG CAGAACGCTT TGAAGAAGGA ATGACGGTAA AGCACTGTGC ATTGTCCCTC 
GTGGGAGAAC CAATAATGTA CCCAGAGATC AACAGGTTTT TGAAGCTACT CCACCAGTGT 
AAAATCTCCA GCTTCCTGGT CACAAATGCA CAATTTCCTG CGGAAATCAG GAACCTCGAG 
CCAGTTACTC AGCTGTATGT CAGTGTGGAT GCCAGTACCA AAGACAGCCT GAAGAAAATC 
GACCGCCCAC TCTTCAAGGA TTTCTGGCAG CAATTCCTTG ACAGTTTAAA AGCCTTGGCA 
GTCAAGCAAC AACGAACTGT CTACAGACTG ATGCTCGTGA AAGCATGGAA CGTGGACGAG 
CTCCAGGCCT ACGCGCAGCT CGTGTCCCTG GGGAATCCTG ACTTCATCGA AGTGAAGGGC 
GTTACCTACT GCAGAGAAAG TTCAGCAAGC AGTCTTACCA TGGCCCATGT GCCCTGGCAT 
GAGGAAGTGG TACAGTTTGT CCGCGAGCTG GTGGATCTGA TCCCCGAATA TGAAATTGCA 
TGTGAACACG AACACTCTAA TTGCCTCCTG ATAGCACACA GAAAGTTTAA AATTGGTGGT 
GAATGGTGGA CATGGATCGA TTATAACCGC TTCCAGGAGC TCATCCAGGA ATATGAAGAT 
AGTGGTGGAT CAAAAACGTT CAGCGCAAAG GATTATATGG CCAGAACTCC TCACTGGGCA 
TTATTTGGTG CCAATGAAAG AAGCTTTGAT CCCAAGGACA CAAGACATCA GAGAAAGAAC 
AAATCAAAGG CTATTTCTGG ATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TYW1B ( XM_011516230.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514532.1 Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu80327
Clone ID Related Accession (Same CDS sequence)	XM_011516230.2 , XM_011516230.3
Accession Version	XM_011516230.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011516230.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTT CTGCGGATAC ATGGGACCTC TCCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATCTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCGAGA TGCAGGGATT TGCAACAGTT CTTGCTGAAG CAGTTACGTC CCTGGATCTG 
CCTGTGGCCA TTATTAATCT AAAAGAATAT GATCCAGATG ATCATCTGAT AGAAGAGGTG 
ACTAGTAAAA ATGTCTGTGT CTTCCTGGTT GCGACATACA CTGACGGCCT ACCAACCGAA 
AGTGCAGAGT GGTTCTGCAA ATGGTTAGAG GAAGCATCCA TTGATTTTCG ATTTGGCAAA 
ACTTACCTGA AGGGTATGAG AGATGCGGTA TTTGGCCTGG GAAATTCTGC CTATGCTAGC 
CACTTCAACA AGGTTGGCAA AAATGTTGAC AAGTGGCTCT GGATGCTTGG CGTGCATCGT 
GTGATGAGTC GAGGGGAGGG CGACTGCGAC GTGGTTAAAA GCAAGCACGG CAGCATTGAG 
GCCAACTTCA GAGCATGGAA GACCAAGTTC ATCTCCCAGC TGCAGGCACT TCAGAAAGGG 
GAGAGAAAGA AGTCCTGTGG CGGCCACTGC AAGAAAGGCA AATGTGAATC TCACCAACAT 
GGCTCAGAGG AGAGGGAGGA AGGATCTCAA GAGCAGGACG AATTGCATCA CAGAGACACC 
AAGGAGGAAG AACCCTTCGA GAGCTCCAGT GAAGAAGAGT TTGGTGGTGA GGACCATCAG 
AGCCTAAATT CCATTGTTGA TGTTGAAGAT TTGGGCAAAA TTATGGATCA CGTGAAGAAA 
GAAAAGAGAG AAAAGGAACA GCAGGAAGAG AAGTCTGGTT TGTTCAGGAA CATGGGGAGG 
AATGAAGATG GTGAAAGAAG AGCTATGATA ACTCCTGCTC TCCGAGAAGC CCTTACTAAA 
CAAGTCGATG CTCCGAGGGA GAGGAGCTTG TTACAAACAC ACATTCTATG GAATGAGAGC 
CATCGCTGCA TGGAAACCAC CCCGAGCTTG GCGTGTGCTA ATAAATGTGT CTTCTGTTGG 
TGGCACCACA ACAACCCTGT GGGCACTGAA TGGTTGTGGA AGATGGACCA GCCTGAAATG 
ATCTTGAAGG AAGCCATTGA AAACCATCAG AACATGATTA AGCAGTTTAA AGGAGTACCG 
GGCGTCAAAG CAGAACGCTT TGAAGAAGGA ATGACGGTAA AGCACTGTGC ATTGTCCCTC 
GTGGGAGAAC CAATAATGTA CCCAGAGATC AACAGGTTTT TGAAGCTACT CCACCAGTGT 
AAAATCTCCA GCTTCCTGGT CACAAATGCA CAATTTCCTG CGGAAATCAG GAACCTCGAG 
CCAGTTACTC AGCTGTATGT CAGTGTGGAT GCCAGTACCA AAGACAGCCT GAAGAAAATC 
GACCGCCCAC TCTTCAAGGA TTTCTGGCAG CAATTCCTTG ACAGTTTAAA AGCCTTGGCA 
GTCAAGCAAC AACGAACTGT CTACAGACTG ATGCTCGTGA AAGCATGGAA CGTGGACGAG 
CTCCAGGCCT ACGCGCAGCT CGTGTCCCTG GGGAATCCTG ACTTCATCGA AGTGAAGGGC 
GTTACCTACT GCAGAGAAAG TTCAGCAAGC AGTCTTACCA TGGCCCATGT GCCCTGGCAT 
GAGGAAGTGG TACAGTTTGT CCGCGAGCTG GTGGATCTGA TCCCCGAATA TGAAATTGCA 
TGTGAACACG AACACTCTAA TTGCCTCCTG ATAGCACACA GAAAGGATTT AGGAAGACTC 
CCCAGAGCAG AGGATGAAGG ACATGTGAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514532.1
CDS	160..1992
Translation	MDPSADTWDLSSPLISLWINRFYIYLGFAVSISLWICVQIVIEMQGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRDAVFGLGNSAYASHFNKVGKNVDKWLWMLGVHRVMSRGEGDCDVVKSKHGSIEANFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSQEQDELHHRDTKEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQVDAPRERSLLQTHILWNESHRCMETTPSLACANKCVFCWWHHNNPVGTEWLWKMDQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQQFLDSLKALAVKQQRTVYRLMLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCRESSASSLTMAHVPWHEEVVQFVRELVDLIPEYEIACEHEHSNCLLIAHRKDLGRLPRAEDEGHVS

Target ORF information:

RefSeq Version	XM_011516230.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011516230.2

ORF Insert Sequence:

ATGGATCCTT CTGCGGATAC ATGGGACCTC TCCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATCTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCGAGA TGCAGGGATT TGCAACAGTT CTTGCTGAAG CAGTTACGTC CCTGGATCTG 
CCTGTGGCCA TTATTAATCT AAAAGAATAT GATCCAGATG ATCATCTGAT AGAAGAGGTG 
ACTAGTAAAA ATGTCTGTGT CTTCCTGGTT GCGACATACA CTGACGGCCT ACCAACCGAA 
AGTGCAGAGT GGTTCTGCAA ATGGTTAGAG GAAGCATCCA TTGATTTTCG ATTTGGCAAA 
ACTTACCTGA AGGGTATGAG AGATGCGGTA TTTGGCCTGG GAAATTCTGC CTATGCTAGC 
CACTTCAACA AGGTTGGCAA AAATGTTGAC AAGTGGCTCT GGATGCTTGG CGTGCATCGT 
GTGATGAGTC GAGGGGAGGG CGACTGCGAC GTGGTTAAAA GCAAGCACGG CAGCATTGAG 
GCCAACTTCA GAGCATGGAA GACCAAGTTC ATCTCCCAGC TGCAGGCACT TCAGAAAGGG 
GAGAGAAAGA AGTCCTGTGG CGGCCACTGC AAGAAAGGCA AATGTGAATC TCACCAACAT 
GGCTCAGAGG AGAGGGAGGA AGGATCTCAA GAGCAGGACG AATTGCATCA CAGAGACACC 
AAGGAGGAAG AACCCTTCGA GAGCTCCAGT GAAGAAGAGT TTGGTGGTGA GGACCATCAG 
AGCCTAAATT CCATTGTTGA TGTTGAAGAT TTGGGCAAAA TTATGGATCA CGTGAAGAAA 
GAAAAGAGAG AAAAGGAACA GCAGGAAGAG AAGTCTGGTT TGTTCAGGAA CATGGGGAGG 
AATGAAGATG GTGAAAGAAG AGCTATGATA ACTCCTGCTC TCCGAGAAGC CCTTACTAAA 
CAAGTCGATG CTCCGAGGGA GAGGAGCTTG TTACAAACAC ACATTCTATG GAATGAGAGC 
CATCGCTGCA TGGAAACCAC CCCGAGCTTG GCGTGTGCTA ATAAATGTGT CTTCTGTTGG 
TGGCACCACA ACAACCCTGT GGGCACTGAA TGGTTGTGGA AGATGGACCA GCCTGAAATG 
ATCTTGAAGG AAGCCATTGA AAACCATCAG AACATGATTA AGCAGTTTAA AGGAGTACCG 
GGCGTCAAAG CAGAACGCTT TGAAGAAGGA ATGACGGTAA AGCACTGTGC ATTGTCCCTC 
GTGGGAGAAC CAATAATGTA CCCAGAGATC AACAGGTTTT TGAAGCTACT CCACCAGTGT 
AAAATCTCCA GCTTCCTGGT CACAAATGCA CAATTTCCTG CGGAAATCAG GAACCTCGAG 
CCAGTTACTC AGCTGTATGT CAGTGTGGAT GCCAGTACCA AAGACAGCCT GAAGAAAATC 
GACCGCCCAC TCTTCAAGGA TTTCTGGCAG CAATTCCTTG ACAGTTTAAA AGCCTTGGCA 
GTCAAGCAAC AACGAACTGT CTACAGACTG ATGCTCGTGA AAGCATGGAA CGTGGACGAG 
CTCCAGGCCT ACGCGCAGCT CGTGTCCCTG GGGAATCCTG ACTTCATCGA AGTGAAGGGC 
GTTACCTACT GCAGAGAAAG TTCAGCAAGC AGTCTTACCA TGGCCCATGT GCCCTGGCAT 
GAGGAAGTGG TACAGTTTGT CCGCGAGCTG GTGGATCTGA TCCCCGAATA TGAAATTGCA 
TGTGAACACG AACACTCTAA TTGCCTCCTG ATAGCACACA GAAAGGATTT AGGAAGACTC 
CCCAGAGCAG AGGATGAAGG ACATGTGAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TYW1B ( XM_011516230.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514532.1 Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X1, mRNA.

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CloneID	OHu80327
Clone ID Related Accession (Same CDS sequence)	XM_011516230.2 , XM_011516230.3
Accession Version	XM_011516230.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011516230.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTT CTGCGGATAC ATGGGACCTC TCCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATCTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCGAGA TGCAGGGATT TGCAACAGTT CTTGCTGAAG CAGTTACGTC CCTGGATCTG 
CCTGTGGCCA TTATTAATCT AAAAGAATAT GATCCAGATG ATCATCTGAT AGAAGAGGTG 
ACTAGTAAAA ATGTCTGTGT CTTCCTGGTT GCGACATACA CTGACGGCCT ACCAACCGAA 
AGTGCAGAGT GGTTCTGCAA ATGGTTAGAG GAAGCATCCA TTGATTTTCG ATTTGGCAAA 
ACTTACCTGA AGGGTATGAG AGATGCGGTA TTTGGCCTGG GAAATTCTGC CTATGCTAGC 
CACTTCAACA AGGTTGGCAA AAATGTTGAC AAGTGGCTCT GGATGCTTGG CGTGCATCGT 
GTGATGAGTC GAGGGGAGGG CGACTGCGAC GTGGTTAAAA GCAAGCACGG CAGCATTGAG 
GCCAACTTCA GAGCATGGAA GACCAAGTTC ATCTCCCAGC TGCAGGCACT TCAGAAAGGG 
GAGAGAAAGA AGTCCTGTGG CGGCCACTGC AAGAAAGGCA AATGTGAATC TCACCAACAT 
GGCTCAGAGG AGAGGGAGGA AGGATCTCAA GAGCAGGACG AATTGCATCA CAGAGACACC 
AAGGAGGAAG AACCCTTCGA GAGCTCCAGT GAAGAAGAGT TTGGTGGTGA GGACCATCAG 
AGCCTAAATT CCATTGTTGA TGTTGAAGAT TTGGGCAAAA TTATGGATCA CGTGAAGAAA 
GAAAAGAGAG AAAAGGAACA GCAGGAAGAG AAGTCTGGTT TGTTCAGGAA CATGGGGAGG 
AATGAAGATG GTGAAAGAAG AGCTATGATA ACTCCTGCTC TCCGAGAAGC CCTTACTAAA 
CAAGTCGATG CTCCGAGGGA GAGGAGCTTG TTACAAACAC ACATTCTATG GAATGAGAGC 
CATCGCTGCA TGGAAACCAC CCCGAGCTTG GCGTGTGCTA ATAAATGTGT CTTCTGTTGG 
TGGCACCACA ACAACCCTGT GGGCACTGAA TGGTTGTGGA AGATGGACCA GCCTGAAATG 
ATCTTGAAGG AAGCCATTGA AAACCATCAG AACATGATTA AGCAGTTTAA AGGAGTACCG 
GGCGTCAAAG CAGAACGCTT TGAAGAAGGA ATGACGGTAA AGCACTGTGC ATTGTCCCTC 
GTGGGAGAAC CAATAATGTA CCCAGAGATC AACAGGTTTT TGAAGCTACT CCACCAGTGT 
AAAATCTCCA GCTTCCTGGT CACAAATGCA CAATTTCCTG CGGAAATCAG GAACCTCGAG 
CCAGTTACTC AGCTGTATGT CAGTGTGGAT GCCAGTACCA AAGACAGCCT GAAGAAAATC 
GACCGCCCAC TCTTCAAGGA TTTCTGGCAG CAATTCCTTG ACAGTTTAAA AGCCTTGGCA 
GTCAAGCAAC AACGAACTGT CTACAGACTG ATGCTCGTGA AAGCATGGAA CGTGGACGAG 
CTCCAGGCCT ACGCGCAGCT CGTGTCCCTG GGGAATCCTG ACTTCATCGA AGTGAAGGGC 
GTTACCTACT GCAGAGAAAG TTCAGCAAGC AGTCTTACCA TGGCCCATGT GCCCTGGCAT 
GAGGAAGTGG TACAGTTTGT CCGCGAGCTG GTGGATCTGA TCCCCGAATA TGAAATTGCA 
TGTGAACACG AACACTCTAA TTGCCTCCTG ATAGCACACA GAAAGGATTT AGGAAGACTC 
CCCAGAGCAG AGGATGAAGG ACATGTGAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514532.1
CDS	160..1992
Translation	MDPSADTWDLSSPLISLWINRFYIYLGFAVSISLWICVQIVIEMQGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRDAVFGLGNSAYASHFNKVGKNVDKWLWMLGVHRVMSRGEGDCDVVKSKHGSIEANFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSQEQDELHHRDTKEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQVDAPRERSLLQTHILWNESHRCMETTPSLACANKCVFCWWHHNNPVGTEWLWKMDQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQQFLDSLKALAVKQQRTVYRLMLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCRESSASSLTMAHVPWHEEVVQFVRELVDLIPEYEIACEHEHSNCLLIAHRKDLGRLPRAEDEGHVS

Target ORF information:

RefSeq Version	XM_011516230.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011516230.3

ORF Insert Sequence:

ATGGATCCTT CTGCGGATAC ATGGGACCTC TCCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATCTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCGAGA TGCAGGGATT TGCAACAGTT CTTGCTGAAG CAGTTACGTC CCTGGATCTG 
CCTGTGGCCA TTATTAATCT AAAAGAATAT GATCCAGATG ATCATCTGAT AGAAGAGGTG 
ACTAGTAAAA ATGTCTGTGT CTTCCTGGTT GCGACATACA CTGACGGCCT ACCAACCGAA 
AGTGCAGAGT GGTTCTGCAA ATGGTTAGAG GAAGCATCCA TTGATTTTCG ATTTGGCAAA 
ACTTACCTGA AGGGTATGAG AGATGCGGTA TTTGGCCTGG GAAATTCTGC CTATGCTAGC 
CACTTCAACA AGGTTGGCAA AAATGTTGAC AAGTGGCTCT GGATGCTTGG CGTGCATCGT 
GTGATGAGTC GAGGGGAGGG CGACTGCGAC GTGGTTAAAA GCAAGCACGG CAGCATTGAG 
GCCAACTTCA GAGCATGGAA GACCAAGTTC ATCTCCCAGC TGCAGGCACT TCAGAAAGGG 
GAGAGAAAGA AGTCCTGTGG CGGCCACTGC AAGAAAGGCA AATGTGAATC TCACCAACAT 
GGCTCAGAGG AGAGGGAGGA AGGATCTCAA GAGCAGGACG AATTGCATCA CAGAGACACC 
AAGGAGGAAG AACCCTTCGA GAGCTCCAGT GAAGAAGAGT TTGGTGGTGA GGACCATCAG 
AGCCTAAATT CCATTGTTGA TGTTGAAGAT TTGGGCAAAA TTATGGATCA CGTGAAGAAA 
GAAAAGAGAG AAAAGGAACA GCAGGAAGAG AAGTCTGGTT TGTTCAGGAA CATGGGGAGG 
AATGAAGATG GTGAAAGAAG AGCTATGATA ACTCCTGCTC TCCGAGAAGC CCTTACTAAA 
CAAGTCGATG CTCCGAGGGA GAGGAGCTTG TTACAAACAC ACATTCTATG GAATGAGAGC 
CATCGCTGCA TGGAAACCAC CCCGAGCTTG GCGTGTGCTA ATAAATGTGT CTTCTGTTGG 
TGGCACCACA ACAACCCTGT GGGCACTGAA TGGTTGTGGA AGATGGACCA GCCTGAAATG 
ATCTTGAAGG AAGCCATTGA AAACCATCAG AACATGATTA AGCAGTTTAA AGGAGTACCG 
GGCGTCAAAG CAGAACGCTT TGAAGAAGGA ATGACGGTAA AGCACTGTGC ATTGTCCCTC 
GTGGGAGAAC CAATAATGTA CCCAGAGATC AACAGGTTTT TGAAGCTACT CCACCAGTGT 
AAAATCTCCA GCTTCCTGGT CACAAATGCA CAATTTCCTG CGGAAATCAG GAACCTCGAG 
CCAGTTACTC AGCTGTATGT CAGTGTGGAT GCCAGTACCA AAGACAGCCT GAAGAAAATC 
GACCGCCCAC TCTTCAAGGA TTTCTGGCAG CAATTCCTTG ACAGTTTAAA AGCCTTGGCA 
GTCAAGCAAC AACGAACTGT CTACAGACTG ATGCTCGTGA AAGCATGGAA CGTGGACGAG 
CTCCAGGCCT ACGCGCAGCT CGTGTCCCTG GGGAATCCTG ACTTCATCGA AGTGAAGGGC 
GTTACCTACT GCAGAGAAAG TTCAGCAAGC AGTCTTACCA TGGCCCATGT GCCCTGGCAT 
GAGGAAGTGG TACAGTTTGT CCGCGAGCTG GTGGATCTGA TCCCCGAATA TGAAATTGCA 
TGTGAACACG AACACTCTAA TTGCCTCCTG ATAGCACACA GAAAGGATTT AGGAAGACTC 
CCCAGAGCAG AGGATGAAGG ACATGTGAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TYW1B ( XM_011516231.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514533.1 Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu80328
Clone ID Related Accession (Same CDS sequence)	XM_011516231.2
Accession Version	XM_011516231.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011516231.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTT CTGCGGATAC ATGGGACCTC TCCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATCTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCGAGA TGCAGGGATT TGCAACAGTT CTTGCTGAAG CAGTTACGTC CCTGGATCTG 
CCTGTGGCCA TTATTAATCT AAAAGAATAT GATCCAGATG ATCATCTGAT AGAAGAGGTG 
ACTAGTAAAA ATGTCTGTGT CTTCCTGGTT GCGACATACA CTGACGGCCT ACCAACCGAA 
AGTGCAGAGT GGTTCTGCAA ATGGTTAGAG GAAGCATCCA TTGATTTTCG ATTTGGCAAA 
ACTTACCTGA AGGGTATGAG AGATGCGGTA TTTGGCCTGG GAAATTCTGC CTATGCTAGC 
CACTTCAACA AGGTTGGCAA AAATGTTGAC AAGTGGCTCT GGATGCTTGG CGTGCATCGT 
GTGATGAGTC GAGGGGAGGG CGACTGCGAC GTGGTTAAAA GCAAGCACGG CAGCATTGAG 
GCCAACTTCA GAGCATGGAA GACCAAGTTC ATCTCCCAGC TGCAGGCACT TCAGAAAGGG 
GAGAGAAAGA AGTCCTGTGG CGGCCACTGC AAGAAAGGCA AATGTGAATC TCACCAACAT 
GGCTCAGAGG AGAGGGAGGA AGGATCTCAA GAGCAGGACG AATTGCATCA CAGAGACACC 
AAGGAGGAAG AACCCTTCGA GAGCTCCAGT GAAGAAGAGT TTGGTGGTGA GGACCATCAG 
AGCCTAAATT CCATTGTTGA TGTTGAAGAT TTGGGCAAAA TTATGGATCA CGTGAAGAAA 
GAAAAGAGAG AAAAGGAACA GCAGGAAGAG AAGTCTGGTT TGTTCAGGAA CATGGGGAGG 
AATGAAGATG GTGAAAGAAG AGCTATGATA ACTCCTGCTC TCCGAGAAGC CCTTACTAAA 
CAAGTCGATG CTCCGAGGGA GAGGAGCTTG TTACAAACAC ACATTCTATG GAATGAGAGC 
CATCGCTGCA TGGAAACCAC CCCGAGCTTG GCGTGTGCTA ATAAATGTGT CTTCTGTTGG 
TGGCACCACA ACAACCCTGT GGGCACTGAA TGGTTGTGGA AGATGGACCA GCCTGAAATG 
ATCTTGAAGG AAGCCATTGA AAACCATCAG AACATGATTA AGCAGTTTAA AGGAGTACCG 
GGCGTCAAAG CAGAACGCTT TGAAGAAGGA ATGACGGTAA AGCACTGTGC ATTGTCCCTC 
GTGGGAGAAC CAATAATGTA CCCAGAGATC AACAGGTTTT TGAAGCTACT CCACCAGTGT 
AAAATCTCCA GCTTCCTGGT CACAAATGCA CAATTTCCTG CGGAAATCAG GAACCTCGAG 
CCAGTTACTC AGCTGTATGT CAGTGTGGAT GCCAGTACCA AAGACAGCCT GAAGAAAATC 
GACCGCCCAC TCTTCAAGGA TTTCTGGCAG CAATTCCTTG ACAGTTTAAA AGCCTTGGCA 
GTCAAGAGGG AATCAGTATC CTGTAATTGG TGTTTATCGT TCCATATCGT CTTCAGTATT 
TCATGTTTGG TGTTTATCAT TCCATATTGT ATTAATGAGG GTTCTCTAGA GGGACAGAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514533.1
CDS	160..1782
Translation	MDPSADTWDLSSPLISLWINRFYIYLGFAVSISLWICVQIVIEMQGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRDAVFGLGNSAYASHFNKVGKNVDKWLWMLGVHRVMSRGEGDCDVVKSKHGSIEANFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSQEQDELHHRDTKEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQVDAPRERSLLQTHILWNESHRCMETTPSLACANKCVFCWWHHNNPVGTEWLWKMDQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQQFLDSLKALAVKRESVSCNWCLSFHIVFSISCLVFIIPYCINEGSLEGQN

Target ORF information:

RefSeq Version	XM_011516231.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011516231.2

ORF Insert Sequence:

ATGGATCCTT CTGCGGATAC ATGGGACCTC TCCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATCTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCGAGA TGCAGGGATT TGCAACAGTT CTTGCTGAAG CAGTTACGTC CCTGGATCTG 
CCTGTGGCCA TTATTAATCT AAAAGAATAT GATCCAGATG ATCATCTGAT AGAAGAGGTG 
ACTAGTAAAA ATGTCTGTGT CTTCCTGGTT GCGACATACA CTGACGGCCT ACCAACCGAA 
AGTGCAGAGT GGTTCTGCAA ATGGTTAGAG GAAGCATCCA TTGATTTTCG ATTTGGCAAA 
ACTTACCTGA AGGGTATGAG AGATGCGGTA TTTGGCCTGG GAAATTCTGC CTATGCTAGC 
CACTTCAACA AGGTTGGCAA AAATGTTGAC AAGTGGCTCT GGATGCTTGG CGTGCATCGT 
GTGATGAGTC GAGGGGAGGG CGACTGCGAC GTGGTTAAAA GCAAGCACGG CAGCATTGAG 
GCCAACTTCA GAGCATGGAA GACCAAGTTC ATCTCCCAGC TGCAGGCACT TCAGAAAGGG 
GAGAGAAAGA AGTCCTGTGG CGGCCACTGC AAGAAAGGCA AATGTGAATC TCACCAACAT 
GGCTCAGAGG AGAGGGAGGA AGGATCTCAA GAGCAGGACG AATTGCATCA CAGAGACACC 
AAGGAGGAAG AACCCTTCGA GAGCTCCAGT GAAGAAGAGT TTGGTGGTGA GGACCATCAG 
AGCCTAAATT CCATTGTTGA TGTTGAAGAT TTGGGCAAAA TTATGGATCA CGTGAAGAAA 
GAAAAGAGAG AAAAGGAACA GCAGGAAGAG AAGTCTGGTT TGTTCAGGAA CATGGGGAGG 
AATGAAGATG GTGAAAGAAG AGCTATGATA ACTCCTGCTC TCCGAGAAGC CCTTACTAAA 
CAAGTCGATG CTCCGAGGGA GAGGAGCTTG TTACAAACAC ACATTCTATG GAATGAGAGC 
CATCGCTGCA TGGAAACCAC CCCGAGCTTG GCGTGTGCTA ATAAATGTGT CTTCTGTTGG 
TGGCACCACA ACAACCCTGT GGGCACTGAA TGGTTGTGGA AGATGGACCA GCCTGAAATG 
ATCTTGAAGG AAGCCATTGA AAACCATCAG AACATGATTA AGCAGTTTAA AGGAGTACCG 
GGCGTCAAAG CAGAACGCTT TGAAGAAGGA ATGACGGTAA AGCACTGTGC ATTGTCCCTC 
GTGGGAGAAC CAATAATGTA CCCAGAGATC AACAGGTTTT TGAAGCTACT CCACCAGTGT 
AAAATCTCCA GCTTCCTGGT CACAAATGCA CAATTTCCTG CGGAAATCAG GAACCTCGAG 
CCAGTTACTC AGCTGTATGT CAGTGTGGAT GCCAGTACCA AAGACAGCCT GAAGAAAATC 
GACCGCCCAC TCTTCAAGGA TTTCTGGCAG CAATTCCTTG ACAGTTTAAA AGCCTTGGCA 
GTCAAGAGGG AATCAGTATC CTGTAATTGG TGTTTATCGT TCCATATCGT CTTCAGTATT 
TCATGTTTGG TGTTTATCAT TCCATATTGT ATTAATGAGG GTTCTCTAGA GGGACAGAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TYW1B ( XM_011516232.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514534.1 Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu80329
Clone ID Related Accession (Same CDS sequence)	XM_011516232.3 , XM_011516232.2
Accession Version	XM_011516232.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011516232.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTT CTGCGGATAC ATGGGACCTC TCCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATCTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCGAGA TGCAGGGATT TGCAACAGTT CTTGCTGAAG CAGTTACGTC CCTGGATCTG 
CCTGTGGCCA TTATTAATCT AAAAGAATAT GATCCAGATG ATCATCTGAT AGAAGAGGTG 
ACTAGTAAAA ATGTCTGTGT CTTCCTGGTT GCGACATACA CTGACGGCCT ACCAACCGAA 
AGTGCAGAGT GGTTCTGCAA ATGGTTAGAG GAAGCATCCA TTGATTTTCG ATTTGGCAAA 
ACTTACCTGA AGGGTATGAG AGATGCGGTA TTTGGCCTGG GAAATTCTGC CTATGCTAGC 
CACTTCAACA AGGTTGGCAA AAATGTTGAC AAGTGGCTCT GGATGCTTGG CGTGCATCGT 
GTGATGAGTC GAGGGGAGGG CGACTGCGAC GTGGTTAAAA GCAAGCACGG CAGCATTGAG 
GCCAACTTCA GAGCATGGAA GACCAAGTTC ATCTCCCAGC TGCAGGCACT TCAGAAAGGG 
GAGAGAAAGA AGTCCTGTGG CGGCCACTGC AAGAAAGGCA AATGTGAATC TCACCAACAT 
GGCTCAGAGG AGAGGGAGGA AGGATCTCAA GAGCAGGACG AATTGCATCA CAGAGACACC 
AAGGAGGAAG AACCCTTCGA GAGCTCCAGT GAAGAAGAGT TTGGTGGTGA GGACCATCAG 
AGCCTAAATT CCATTGTTGA TGTTGAAGAT TTGGGCAAAA TTATGGATCA CGTGAAGAAA 
GAAAAGAGAG AAAAGGAACA GCAGGAAGAG AAGTCTGGTT TGTTCAGGAA CATGGGGAGG 
AATGAAGATG GTGAAAGAAG AGCTATGATA ACTCCTGCTC TCCGAGAAGC CCTTACTAAA 
CAAGTCGATG CTCCGAGGGA GAGGAGCTTG TTACAAACAC ACATTCTATG GAATGAGAGC 
CATCGCTGCA TGGAAACCAC CCCGAGCTTG GCGTGTGCTA ATAAATGTGT CTTCTGTTGG 
TGGCACCACA ACAACCCTGT GGGCACTGAA TGGTTGTGGA AGATGGACCA GCCTGAAATG 
ATCTTGAAGG AAGCCATTGA AAACCATCAG AACATGATTA AGCAGTTTAA AGCTTTGGAC 
CAACTACATT TACTTTCCCA GTTCAGGATA CTTGAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514534.1
CDS	160..1401
Translation	MDPSADTWDLSSPLISLWINRFYIYLGFAVSISLWICVQIVIEMQGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRDAVFGLGNSAYASHFNKVGKNVDKWLWMLGVHRVMSRGEGDCDVVKSKHGSIEANFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSQEQDELHHRDTKEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQVDAPRERSLLQTHILWNESHRCMETTPSLACANKCVFCWWHHNNPVGTEWLWKMDQPEMILKEAIENHQNMIKQFKALDQLHLLSQFRILEE

Target ORF information:

RefSeq Version	XM_011516232.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011516232.3

ORF Insert Sequence:

ATGGATCCTT CTGCGGATAC ATGGGACCTC TCCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATCTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCGAGA TGCAGGGATT TGCAACAGTT CTTGCTGAAG CAGTTACGTC CCTGGATCTG 
CCTGTGGCCA TTATTAATCT AAAAGAATAT GATCCAGATG ATCATCTGAT AGAAGAGGTG 
ACTAGTAAAA ATGTCTGTGT CTTCCTGGTT GCGACATACA CTGACGGCCT ACCAACCGAA 
AGTGCAGAGT GGTTCTGCAA ATGGTTAGAG GAAGCATCCA TTGATTTTCG ATTTGGCAAA 
ACTTACCTGA AGGGTATGAG AGATGCGGTA TTTGGCCTGG GAAATTCTGC CTATGCTAGC 
CACTTCAACA AGGTTGGCAA AAATGTTGAC AAGTGGCTCT GGATGCTTGG CGTGCATCGT 
GTGATGAGTC GAGGGGAGGG CGACTGCGAC GTGGTTAAAA GCAAGCACGG CAGCATTGAG 
GCCAACTTCA GAGCATGGAA GACCAAGTTC ATCTCCCAGC TGCAGGCACT TCAGAAAGGG 
GAGAGAAAGA AGTCCTGTGG CGGCCACTGC AAGAAAGGCA AATGTGAATC TCACCAACAT 
GGCTCAGAGG AGAGGGAGGA AGGATCTCAA GAGCAGGACG AATTGCATCA CAGAGACACC 
AAGGAGGAAG AACCCTTCGA GAGCTCCAGT GAAGAAGAGT TTGGTGGTGA GGACCATCAG 
AGCCTAAATT CCATTGTTGA TGTTGAAGAT TTGGGCAAAA TTATGGATCA CGTGAAGAAA 
GAAAAGAGAG AAAAGGAACA GCAGGAAGAG AAGTCTGGTT TGTTCAGGAA CATGGGGAGG 
AATGAAGATG GTGAAAGAAG AGCTATGATA ACTCCTGCTC TCCGAGAAGC CCTTACTAAA 
CAAGTCGATG CTCCGAGGGA GAGGAGCTTG TTACAAACAC ACATTCTATG GAATGAGAGC 
CATCGCTGCA TGGAAACCAC CCCGAGCTTG GCGTGTGCTA ATAAATGTGT CTTCTGTTGG 
TGGCACCACA ACAACCCTGT GGGCACTGAA TGGTTGTGGA AGATGGACCA GCCTGAAATG 
ATCTTGAAGG AAGCCATTGA AAACCATCAG AACATGATTA AGCAGTTTAA AGCTTTGGAC 
CAACTACATT TACTTTCCCA GTTCAGGATA CTTGAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TYW1B ( XM_011516232.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514534.1 Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu80329
Clone ID Related Accession (Same CDS sequence)	XM_011516232.3 , XM_011516232.2
Accession Version	XM_011516232.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011516232.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTT CTGCGGATAC ATGGGACCTC TCCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATCTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCGAGA TGCAGGGATT TGCAACAGTT CTTGCTGAAG CAGTTACGTC CCTGGATCTG 
CCTGTGGCCA TTATTAATCT AAAAGAATAT GATCCAGATG ATCATCTGAT AGAAGAGGTG 
ACTAGTAAAA ATGTCTGTGT CTTCCTGGTT GCGACATACA CTGACGGCCT ACCAACCGAA 
AGTGCAGAGT GGTTCTGCAA ATGGTTAGAG GAAGCATCCA TTGATTTTCG ATTTGGCAAA 
ACTTACCTGA AGGGTATGAG AGATGCGGTA TTTGGCCTGG GAAATTCTGC CTATGCTAGC 
CACTTCAACA AGGTTGGCAA AAATGTTGAC AAGTGGCTCT GGATGCTTGG CGTGCATCGT 
GTGATGAGTC GAGGGGAGGG CGACTGCGAC GTGGTTAAAA GCAAGCACGG CAGCATTGAG 
GCCAACTTCA GAGCATGGAA GACCAAGTTC ATCTCCCAGC TGCAGGCACT TCAGAAAGGG 
GAGAGAAAGA AGTCCTGTGG CGGCCACTGC AAGAAAGGCA AATGTGAATC TCACCAACAT 
GGCTCAGAGG AGAGGGAGGA AGGATCTCAA GAGCAGGACG AATTGCATCA CAGAGACACC 
AAGGAGGAAG AACCCTTCGA GAGCTCCAGT GAAGAAGAGT TTGGTGGTGA GGACCATCAG 
AGCCTAAATT CCATTGTTGA TGTTGAAGAT TTGGGCAAAA TTATGGATCA CGTGAAGAAA 
GAAAAGAGAG AAAAGGAACA GCAGGAAGAG AAGTCTGGTT TGTTCAGGAA CATGGGGAGG 
AATGAAGATG GTGAAAGAAG AGCTATGATA ACTCCTGCTC TCCGAGAAGC CCTTACTAAA 
CAAGTCGATG CTCCGAGGGA GAGGAGCTTG TTACAAACAC ACATTCTATG GAATGAGAGC 
CATCGCTGCA TGGAAACCAC CCCGAGCTTG GCGTGTGCTA ATAAATGTGT CTTCTGTTGG 
TGGCACCACA ACAACCCTGT GGGCACTGAA TGGTTGTGGA AGATGGACCA GCCTGAAATG 
ATCTTGAAGG AAGCCATTGA AAACCATCAG AACATGATTA AGCAGTTTAA AGCTTTGGAC 
CAACTACATT TACTTTCCCA GTTCAGGATA CTTGAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514534.1
CDS	160..1401
Translation	MDPSADTWDLSSPLISLWINRFYIYLGFAVSISLWICVQIVIEMQGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRDAVFGLGNSAYASHFNKVGKNVDKWLWMLGVHRVMSRGEGDCDVVKSKHGSIEANFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSQEQDELHHRDTKEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQVDAPRERSLLQTHILWNESHRCMETTPSLACANKCVFCWWHHNNPVGTEWLWKMDQPEMILKEAIENHQNMIKQFKALDQLHLLSQFRILEE

Target ORF information:

RefSeq Version	XM_011516232.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011516232.2

ORF Insert Sequence:

ATGGATCCTT CTGCGGATAC ATGGGACCTC TCCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATCTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCGAGA TGCAGGGATT TGCAACAGTT CTTGCTGAAG CAGTTACGTC CCTGGATCTG 
CCTGTGGCCA TTATTAATCT AAAAGAATAT GATCCAGATG ATCATCTGAT AGAAGAGGTG 
ACTAGTAAAA ATGTCTGTGT CTTCCTGGTT GCGACATACA CTGACGGCCT ACCAACCGAA 
AGTGCAGAGT GGTTCTGCAA ATGGTTAGAG GAAGCATCCA TTGATTTTCG ATTTGGCAAA 
ACTTACCTGA AGGGTATGAG AGATGCGGTA TTTGGCCTGG GAAATTCTGC CTATGCTAGC 
CACTTCAACA AGGTTGGCAA AAATGTTGAC AAGTGGCTCT GGATGCTTGG CGTGCATCGT 
GTGATGAGTC GAGGGGAGGG CGACTGCGAC GTGGTTAAAA GCAAGCACGG CAGCATTGAG 
GCCAACTTCA GAGCATGGAA GACCAAGTTC ATCTCCCAGC TGCAGGCACT TCAGAAAGGG 
GAGAGAAAGA AGTCCTGTGG CGGCCACTGC AAGAAAGGCA AATGTGAATC TCACCAACAT 
GGCTCAGAGG AGAGGGAGGA AGGATCTCAA GAGCAGGACG AATTGCATCA CAGAGACACC 
AAGGAGGAAG AACCCTTCGA GAGCTCCAGT GAAGAAGAGT TTGGTGGTGA GGACCATCAG 
AGCCTAAATT CCATTGTTGA TGTTGAAGAT TTGGGCAAAA TTATGGATCA CGTGAAGAAA 
GAAAAGAGAG AAAAGGAACA GCAGGAAGAG AAGTCTGGTT TGTTCAGGAA CATGGGGAGG 
AATGAAGATG GTGAAAGAAG AGCTATGATA ACTCCTGCTC TCCGAGAAGC CCTTACTAAA 
CAAGTCGATG CTCCGAGGGA GAGGAGCTTG TTACAAACAC ACATTCTATG GAATGAGAGC 
CATCGCTGCA TGGAAACCAC CCCGAGCTTG GCGTGTGCTA ATAAATGTGT CTTCTGTTGG 
TGGCACCACA ACAACCCTGT GGGCACTGAA TGGTTGTGGA AGATGGACCA GCCTGAAATG 
ATCTTGAAGG AAGCCATTGA AAACCATCAG AACATGATTA AGCAGTTTAA AGCTTTGGAC 
CAACTACATT TACTTTCCCA GTTCAGGATA CTTGAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TYW1B ( XM_011516233.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514535.1 Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu80330
Clone ID Related Accession (Same CDS sequence)	XM_011516233.2
Accession Version	XM_011516233.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011516233.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGGA ATGAAGATGG TGAAAGAAGA GCTATGATAA CTCCTGCTCT CCGAGAAGCC 
CTTACTAAAC AAGTCGATGC TCCGAGGGAG AGGAGCTTGT TACAAACACA CATTCTATGG 
AATGAGAGCC ATCGCTGCAT GGAAACCACC CCGAGCTTGG CGTGTGCTAA TAAATGTGTC 
TTCTGTTGGT GGCACCACAA CAACCCTGTG GGCACTGAAT GGTTGTGGAA GATGGACCAG 
CCTGAAATGA TCTTGAAGGA AGCCATTGAA AACCATCAGA ACATGATTAA GCAGTTTAAA 
GGAGTACCGG GCGTCAAAGC AGAACGCTTT GAAGAAGGAA TGACGGTAAA GCACTGTGCA 
TTGTCCCTCG TGGGAGAACC AATAATGTAC CCAGAGATCA ACAGGTTTTT GAAGCTACTC 
CACCAGTGTA AAATCTCCAG CTTCCTGGTC ACAAATGCAC AATTTCCTGC GGAAATCAGG 
AACCTCGAGC CAGTTACTCA GCTGTATGTC AGTGTGGATG CCAGTACCAA AGACAGCCTG 
AAGAAAATCG ACCGCCCACT CTTCAAGGAT TTCTGGCAGC AATTCCTTGA CAGTTTAAAA 
GCCTTGGCAG TCAAGCAACA ACGAACTGTC TACAGACTGA TGCTCGTGAA AGCATGGAAC 
GTGGACGAGC TCCAGGCCTA CGCGCAGCTC GTGTCCCTGG GGAATCCTGA CTTCATCGAA 
GTGAAGGGCG TTACCTACTG CAGAGAAAGT TCAGCAAGCA GTCTTACCAT GGCCCATGTG 
CCCTGGCATG AGGAAGTGGT ACAGTTTGTC CGCGAGCTGG TGGATCTGAT CCCCGAATAT 
GAAATTGCAT GTGAACACGA ACACTCTAAT TGCCTCCTGA TAGCACACAG AAAGTTTAAA 
ATTGGTGGTG AATGGTGGAC ATGGATCGAT TATAACCGCT TCCAGGAGCT CATCCAGGAA 
TATGAAGATA GTGGTGGATC AAAAACGTTC AGCGCAAAGG ATTATATGGC CAGAACTCCT 
CACTGGGCAT TATTTGGTGC CAATGAAAGA AGCTTTGATC CCAAGGACAC AAGACATCAG 
AGAAAGAACA AATCAAAGGC TATTTCTGGA TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514535.1
CDS	69..1184
Translation	MGRNEDGERRAMITPALREALTKQVDAPRERSLLQTHILWNESHRCMETTPSLACANKCVFCWWHHNNPVGTEWLWKMDQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQQFLDSLKALAVKQQRTVYRLMLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCRESSASSLTMAHVPWHEEVVQFVRELVDLIPEYEIACEHEHSNCLLIAHRKFKIGGEWWTWIDYNRFQELIQEYEDSGGSKTFSAKDYMARTPHWALFGANERSFDPKDTRHQRKNKSKAISGC

Target ORF information:

RefSeq Version	XM_011516233.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011516233.2

ORF Insert Sequence:

ATGGGGAGGA ATGAAGATGG TGAAAGAAGA GCTATGATAA CTCCTGCTCT CCGAGAAGCC 
CTTACTAAAC AAGTCGATGC TCCGAGGGAG AGGAGCTTGT TACAAACACA CATTCTATGG 
AATGAGAGCC ATCGCTGCAT GGAAACCACC CCGAGCTTGG CGTGTGCTAA TAAATGTGTC 
TTCTGTTGGT GGCACCACAA CAACCCTGTG GGCACTGAAT GGTTGTGGAA GATGGACCAG 
CCTGAAATGA TCTTGAAGGA AGCCATTGAA AACCATCAGA ACATGATTAA GCAGTTTAAA 
GGAGTACCGG GCGTCAAAGC AGAACGCTTT GAAGAAGGAA TGACGGTAAA GCACTGTGCA 
TTGTCCCTCG TGGGAGAACC AATAATGTAC CCAGAGATCA ACAGGTTTTT GAAGCTACTC 
CACCAGTGTA AAATCTCCAG CTTCCTGGTC ACAAATGCAC AATTTCCTGC GGAAATCAGG 
AACCTCGAGC CAGTTACTCA GCTGTATGTC AGTGTGGATG CCAGTACCAA AGACAGCCTG 
AAGAAAATCG ACCGCCCACT CTTCAAGGAT TTCTGGCAGC AATTCCTTGA CAGTTTAAAA 
GCCTTGGCAG TCAAGCAACA ACGAACTGTC TACAGACTGA TGCTCGTGAA AGCATGGAAC 
GTGGACGAGC TCCAGGCCTA CGCGCAGCTC GTGTCCCTGG GGAATCCTGA CTTCATCGAA 
GTGAAGGGCG TTACCTACTG CAGAGAAAGT TCAGCAAGCA GTCTTACCAT GGCCCATGTG 
CCCTGGCATG AGGAAGTGGT ACAGTTTGTC CGCGAGCTGG TGGATCTGAT CCCCGAATAT 
GAAATTGCAT GTGAACACGA ACACTCTAAT TGCCTCCTGA TAGCACACAG AAAGTTTAAA 
ATTGGTGGTG AATGGTGGAC ATGGATCGAT TATAACCGCT TCCAGGAGCT CATCCAGGAA 
TATGAAGATA GTGGTGGATC AAAAACGTTC AGCGCAAAGG ATTATATGGC CAGAACTCCT 
CACTGGGCAT TATTTGGTGC CAATGAAAGA AGCTTTGATC CCAAGGACAC AAGACATCAG 
AGAAAGAACA AATCAAAGGC TATTTCTGGA TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TYW1B ( XM_017012222.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867711.1 Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu97262
Clone ID Related Accession (Same CDS sequence)	XM_017012222.1 , XM_017012222.2
Accession Version	XM_017012222.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTT CTGCGGATAC ATGGGACCTC TCCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATCTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCGAGA TGCAGGGATT TGCAACAGTT CTTGCTGAAG CAGTTACGTC CCTGGATCTG 
CCTGTGGCCA TTATTAATCT AAAAGAATAT GATCCAGATG ATCATCTGAT AGAAGAGGTG 
ACTAGTAAAA ATGTCTGTGT CTTCCTGGTT GCGACATACA CTGACGGCCT ACCAACCGAA 
AGTGCAGAGT GGTTCTGCAA ATGGTTAGAG GAAGCATCCA TTGATTTTCG ATTTGGCAAA 
ACTTACCTGA AGGGTATGAG AGATGCGGTA TTTGGCCTGG GAAATTCTGC CTATGCTAGC 
CACTTCAACA AGGTTGGCAA AAATGTTGAC AAGTGGCTCT GGATGCTTGG CGTGCATCGT 
GTGATGAGTC GAGGGGAGGG CGACTGCGAC GTGGTTAAAA GCAAGCACGG CAGCATTGAG 
GCCAACTTCA GAGCATGGAA GACCAAGTTC ATCTCCCAGC TGCAGGCACT TCAGAAAGGG 
GAGAGAAAGA AGTCCTGTGG CGGCCACTGC AAGAAAGGCA AATGTGAATC TCACCAACAT 
GGCTCAGAGG AGAGGGAGGA AGGATCTCAA GAGCAGGACG AATTGCATCA CAGAGACACC 
AAGGAGGAAG AACCCTTCGA GAGCTCCAGT GAAGAAGAGT TTGGTGGTGA GGACCATCAG 
AGCCTAAATT CCATTGTTGA TGTTGAAGAT TTGGGCAAAA TTATGGATCA CGTGAAGAAA 
GAAAAGAGAG AAAAGGAACA GCAGGAAGAG AAGTCTGGTT TGTTCAGGAA CATGGGGAGG 
AATGAAGATG GTGAAAGAAG AGCTATGATA ACTCCTGCTC TCCGAGAAGC CCTTACTAAA 
CAAGGTTATC AGTTGATTGG GAGCCACTCA GGGGTGAAGC TTTGCAGGTT TTTTTTGTTT 
GTTTTTATTC AGTATGTCTA TTACATGCAA CTTTTAAATA GCTATTATTA TACACTGTCA 
ATAAATTTAC TATTTAGAGG TATAATTTAC ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867711.1
CDS	160..1275
Translation	MDPSADTWDLSSPLISLWINRFYIYLGFAVSISLWICVQIVIEMQGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRDAVFGLGNSAYASHFNKVGKNVDKWLWMLGVHRVMSRGEGDCDVVKSKHGSIEANFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSQEQDELHHRDTKEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQGYQLIGSHSGVKLCRFFLFVFIQYVYYMQLLNSYYYTLSINLLFRGIIYI

Target ORF information:

RefSeq Version	XM_017012222.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012222.1

ORF Insert Sequence:

ATGGATCCTT CTGCGGATAC ATGGGACCTC TCCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATCTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCGAGA TGCAGGGATT TGCAACAGTT CTTGCTGAAG CAGTTACGTC CCTGGATCTG 
CCTGTGGCCA TTATTAATCT AAAAGAATAT GATCCAGATG ATCATCTGAT AGAAGAGGTG 
ACTAGTAAAA ATGTCTGTGT CTTCCTGGTT GCGACATACA CTGACGGCCT ACCAACCGAA 
AGTGCAGAGT GGTTCTGCAA ATGGTTAGAG GAAGCATCCA TTGATTTTCG ATTTGGCAAA 
ACTTACCTGA AGGGTATGAG AGATGCGGTA TTTGGCCTGG GAAATTCTGC CTATGCTAGC 
CACTTCAACA AGGTTGGCAA AAATGTTGAC AAGTGGCTCT GGATGCTTGG CGTGCATCGT 
GTGATGAGTC GAGGGGAGGG CGACTGCGAC GTGGTTAAAA GCAAGCACGG CAGCATTGAG 
GCCAACTTCA GAGCATGGAA GACCAAGTTC ATCTCCCAGC TGCAGGCACT TCAGAAAGGG 
GAGAGAAAGA AGTCCTGTGG CGGCCACTGC AAGAAAGGCA AATGTGAATC TCACCAACAT 
GGCTCAGAGG AGAGGGAGGA AGGATCTCAA GAGCAGGACG AATTGCATCA CAGAGACACC 
AAGGAGGAAG AACCCTTCGA GAGCTCCAGT GAAGAAGAGT TTGGTGGTGA GGACCATCAG 
AGCCTAAATT CCATTGTTGA TGTTGAAGAT TTGGGCAAAA TTATGGATCA CGTGAAGAAA 
GAAAAGAGAG AAAAGGAACA GCAGGAAGAG AAGTCTGGTT TGTTCAGGAA CATGGGGAGG 
AATGAAGATG GTGAAAGAAG AGCTATGATA ACTCCTGCTC TCCGAGAAGC CCTTACTAAA 
CAAGGTTATC AGTTGATTGG GAGCCACTCA GGGGTGAAGC TTTGCAGGTT TTTTTTGTTT 
GTTTTTATTC AGTATGTCTA TTACATGCAA CTTTTAAATA GCTATTATTA TACACTGTCA 
ATAAATTTAC TATTTAGAGG TATAATTTAC ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TYW1B ( XM_017012222.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867711.1 Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X6, mRNA.

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CloneID	OHu97262
Clone ID Related Accession (Same CDS sequence)	XM_017012222.1 , XM_017012222.2
Accession Version	XM_017012222.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017012222.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTT CTGCGGATAC ATGGGACCTC TCCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATCTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCGAGA TGCAGGGATT TGCAACAGTT CTTGCTGAAG CAGTTACGTC CCTGGATCTG 
CCTGTGGCCA TTATTAATCT AAAAGAATAT GATCCAGATG ATCATCTGAT AGAAGAGGTG 
ACTAGTAAAA ATGTCTGTGT CTTCCTGGTT GCGACATACA CTGACGGCCT ACCAACCGAA 
AGTGCAGAGT GGTTCTGCAA ATGGTTAGAG GAAGCATCCA TTGATTTTCG ATTTGGCAAA 
ACTTACCTGA AGGGTATGAG AGATGCGGTA TTTGGCCTGG GAAATTCTGC CTATGCTAGC 
CACTTCAACA AGGTTGGCAA AAATGTTGAC AAGTGGCTCT GGATGCTTGG CGTGCATCGT 
GTGATGAGTC GAGGGGAGGG CGACTGCGAC GTGGTTAAAA GCAAGCACGG CAGCATTGAG 
GCCAACTTCA GAGCATGGAA GACCAAGTTC ATCTCCCAGC TGCAGGCACT TCAGAAAGGG 
GAGAGAAAGA AGTCCTGTGG CGGCCACTGC AAGAAAGGCA AATGTGAATC TCACCAACAT 
GGCTCAGAGG AGAGGGAGGA AGGATCTCAA GAGCAGGACG AATTGCATCA CAGAGACACC 
AAGGAGGAAG AACCCTTCGA GAGCTCCAGT GAAGAAGAGT TTGGTGGTGA GGACCATCAG 
AGCCTAAATT CCATTGTTGA TGTTGAAGAT TTGGGCAAAA TTATGGATCA CGTGAAGAAA 
GAAAAGAGAG AAAAGGAACA GCAGGAAGAG AAGTCTGGTT TGTTCAGGAA CATGGGGAGG 
AATGAAGATG GTGAAAGAAG AGCTATGATA ACTCCTGCTC TCCGAGAAGC CCTTACTAAA 
CAAGGTTATC AGTTGATTGG GAGCCACTCA GGGGTGAAGC TTTGCAGGTT TTTTTTGTTT 
GTTTTTATTC AGTATGTCTA TTACATGCAA CTTTTAAATA GCTATTATTA TACACTGTCA 
ATAAATTTAC TATTTAGAGG TATAATTTAC ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867711.1
CDS	160..1275
Translation	MDPSADTWDLSSPLISLWINRFYIYLGFAVSISLWICVQIVIEMQGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRDAVFGLGNSAYASHFNKVGKNVDKWLWMLGVHRVMSRGEGDCDVVKSKHGSIEANFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSQEQDELHHRDTKEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQGYQLIGSHSGVKLCRFFLFVFIQYVYYMQLLNSYYYTLSINLLFRGIIYI

Target ORF information:

RefSeq Version	XM_017012222.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012222.2

ORF Insert Sequence:

ATGGATCCTT CTGCGGATAC ATGGGACCTC TCCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATCTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCGAGA TGCAGGGATT TGCAACAGTT CTTGCTGAAG CAGTTACGTC CCTGGATCTG 
CCTGTGGCCA TTATTAATCT AAAAGAATAT GATCCAGATG ATCATCTGAT AGAAGAGGTG 
ACTAGTAAAA ATGTCTGTGT CTTCCTGGTT GCGACATACA CTGACGGCCT ACCAACCGAA 
AGTGCAGAGT GGTTCTGCAA ATGGTTAGAG GAAGCATCCA TTGATTTTCG ATTTGGCAAA 
ACTTACCTGA AGGGTATGAG AGATGCGGTA TTTGGCCTGG GAAATTCTGC CTATGCTAGC 
CACTTCAACA AGGTTGGCAA AAATGTTGAC AAGTGGCTCT GGATGCTTGG CGTGCATCGT 
GTGATGAGTC GAGGGGAGGG CGACTGCGAC GTGGTTAAAA GCAAGCACGG CAGCATTGAG 
GCCAACTTCA GAGCATGGAA GACCAAGTTC ATCTCCCAGC TGCAGGCACT TCAGAAAGGG 
GAGAGAAAGA AGTCCTGTGG CGGCCACTGC AAGAAAGGCA AATGTGAATC TCACCAACAT 
GGCTCAGAGG AGAGGGAGGA AGGATCTCAA GAGCAGGACG AATTGCATCA CAGAGACACC 
AAGGAGGAAG AACCCTTCGA GAGCTCCAGT GAAGAAGAGT TTGGTGGTGA GGACCATCAG 
AGCCTAAATT CCATTGTTGA TGTTGAAGAT TTGGGCAAAA TTATGGATCA CGTGAAGAAA 
GAAAAGAGAG AAAAGGAACA GCAGGAAGAG AAGTCTGGTT TGTTCAGGAA CATGGGGAGG 
AATGAAGATG GTGAAAGAAG AGCTATGATA ACTCCTGCTC TCCGAGAAGC CCTTACTAAA 
CAAGGTTATC AGTTGATTGG GAGCCACTCA GGGGTGAAGC TTTGCAGGTT TTTTTTGTTT 
GTTTTTATTC AGTATGTCTA TTACATGCAA CTTTTAAATA GCTATTATTA TACACTGTCA 
ATAAATTTAC TATTTAGAGG TATAATTTAC ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TYW1B ( NM_001145440.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001138912.2 Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), mRNA.

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CloneID	OHu25170
Clone ID Related Accession (Same CDS sequence)	NM_001145440.2 , NM_001145440.3
Accession Version	NM_001145440.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-02
Organism	Homo sapiens(human)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA769764.1, BC068520.1, BC106019.1 and AI984113.1. On May 2, 2014 this sequence version replaced NM_001145440.1. ##Evidence-Data-START## Transcript exon combination :: BC068520.1, SRR1660803.235670.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATCCTT CTGCGGATAC ATGGGACCTC TCCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATCTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCGAGA TGCAGGGATT TGCAACAGTT CTTGCTGAAG CAGTTACGTC CCTGGATCTG 
CCTGTGGCCA TTATTAATCT AAAAGAATAT GATCCAGATG ATCATCTGAT AGAAGAGGTG 
ACTAGTAAAA ATGTCTGTGT CTTCCTGGTT GCGACATACA CTGACGGCCT ACCAACCGAA 
AGTGCAGAGT GGTTCTGCAA ATGGTTAGAG GAAGCATCCA TTGATTTTCG ATTTGGCAAA 
ACTTACCTGA AGGGTATGAG AGATGCGGTA TTTGGCCTGG GAAATTCTGC CTATGCTAGC 
CACTTCAACA AGGTTGGCAA AAATGTTGAC AAGTGGCTCT GGATGCTTGG CGTGCATCGT 
GTGATGAGTC GAGGGGAGGG CGACTGCGAC GTGGTTAAAA GCAAGCACGG CAGCATTGAG 
GCCAACTTCA GAGCATGGAA GACCAAGTTC ATCTCCCAGC TGCAGGCACT TCAGAAAGGG 
GAGAGAAAGA AGTCCTGTGG CGGCCACTGC AAGAAAGGCA AATGTGAATC TCACCAACAT 
GGCTCAGAGG AGAGGGAGGA AGGATCTCAA GAGCAGGACG AATTGCATCA CAGAGACACC 
AAGGAGGAAG AACCCTTCGA GAGCTCCAGT GAAGAAGAGT TTGGTGGTGA GGACCATCAG 
AGCCTAAATT CCATTGTTGA TGTTGAAGAT TTGGGCAAAA TTATGGATCA CGTGAAGAAA 
GAAAAGAGAG AAAAGGAACA GCAGGAAGAG AAGTCTGGTT TGTTCAGGAA CATGGGGAGG 
AATGAAGATG GTGAAAGAAG AGCTATGATA ACTCCTGCTC TCCGAGAAGC CCTTACTAAA 
CAAGTCGATG CTCCGAGGGA GAGGAGCTTG TTACAAACAC ACATTCTATG GAATGAGAGC 
CATCGCTGCA TGGAAACCAC CCCGAGCTTG GCGTGTGCTA ATAAATGTGT CTTCTGTTGG 
TGGCACCACA ACAACCCTGT GGGCACTGAA TGGTTGTGGA AGATGGACCA GCCTGAAATG 
ATCTTGAAGG AAGCCATTGA AAACCATCAG AACATGATTA AGCAGTTTAA AGGAGTACCG 
GGCGTCAAAG CAGAACGCTT TGAAGAAGGA ATGACGGTAA AGCACTGTGC ATTGTCCCTC 
GTGGGAGAAC CAATAATGTA CCCAGAGATC AACAGGTTTT TGAAGCTACT CCACCAGTGT 
AAAATCTCCA GCTTCCTGGT CACAAATGCA CAATTTCCTG CGGAAATCAG GAACCTCGAG 
CCAGTTACTC AGCTGTATGT CAGTGTGGAT GCCAGTACCA AAGACAGCCT GAAGAAAATC 
GACCGCCCAC TCTTCAAGGA TTTCTGGCAG CAATTCCTTG ACAGTTTAAA AGCCTTGGCA 
GTCAAGCAAC AACGAACTGT CTACAGACTG ATGCTCGTGA AAGCATGGAA CGTGGACGAG 
CTCCAGGCCT ACGCGCAGCT CGTGTCCCTG GGGAATCCTG ACTTCATCGA AGTGAAGGGC 
GTTACCTACT GCAGAGAAAG TTCAGCAAGC AGTCTTACCA TGGCCCATGT GCCCTGGCAT 
GAGGAAGTGG TACAGTTTGT CCGCGAGCTG GTGGATCTGA TCCCCGAATA TGAAATTGCA 
TGTGAACACG AACACTCTAA TTGCCTCCTG ATAGCACACA GAAAGTTTAA AATTGGTGGT 
GAATGGTGGA CATGGATCGA TTATAACCGC TTCCAGGAGC TCATCCAGGA ATATGAAGAT 
AGTGGTGGAT CAAAAACGTT CAGCGCAAAG GATTATATGG CCAGAACTCC TCACTGGGCA 
TTATTTGGTG CCAATGAAAG AAGCTTTGAT CCCAAGGACA CAAGACATCA GAGAAAGAAC 
AAATCAAAGG CTATTTCTGG ATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138912.2
CDS	160..2166
Translation	MDPSADTWDLSSPLISLWINRFYIYLGFAVSISLWICVQIVIEMQGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRDAVFGLGNSAYASHFNKVGKNVDKWLWMLGVHRVMSRGEGDCDVVKSKHGSIEANFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSQEQDELHHRDTKEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQVDAPRERSLLQTHILWNESHRCMETTPSLACANKCVFCWWHHNNPVGTEWLWKMDQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQQFLDSLKALAVKQQRTVYRLMLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCRESSASSLTMAHVPWHEEVVQFVRELVDLIPEYEIACEHEHSNCLLIAHRKFKIGGEWWTWIDYNRFQELIQEYEDSGGSKTFSAKDYMARTPHWALFGANERSFDPKDTRHQRKNKSKAISGC

Target ORF information:

RefSeq Version	NM_001145440.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145440.2

ORF Insert Sequence:

ATGGATCCTT CTGCGGATAC ATGGGACCTC TCCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATCTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCGAGA TGCAGGGATT TGCAACAGTT CTTGCTGAAG CAGTTACGTC CCTGGATCTG 
CCTGTGGCCA TTATTAATCT AAAAGAATAT GATCCAGATG ATCATCTGAT AGAAGAGGTG 
ACTAGTAAAA ATGTCTGTGT CTTCCTGGTT GCGACATACA CTGACGGCCT ACCAACCGAA 
AGTGCAGAGT GGTTCTGCAA ATGGTTAGAG GAAGCATCCA TTGATTTTCG ATTTGGCAAA 
ACTTACCTGA AGGGTATGAG AGATGCGGTA TTTGGCCTGG GAAATTCTGC CTATGCTAGC 
CACTTCAACA AGGTTGGCAA AAATGTTGAC AAGTGGCTCT GGATGCTTGG CGTGCATCGT 
GTGATGAGTC GAGGGGAGGG CGACTGCGAC GTGGTTAAAA GCAAGCACGG CAGCATTGAG 
GCCAACTTCA GAGCATGGAA GACCAAGTTC ATCTCCCAGC TGCAGGCACT TCAGAAAGGG 
GAGAGAAAGA AGTCCTGTGG CGGCCACTGC AAGAAAGGCA AATGTGAATC TCACCAACAT 
GGCTCAGAGG AGAGGGAGGA AGGATCTCAA GAGCAGGACG AATTGCATCA CAGAGACACC 
AAGGAGGAAG AACCCTTCGA GAGCTCCAGT GAAGAAGAGT TTGGTGGTGA GGACCATCAG 
AGCCTAAATT CCATTGTTGA TGTTGAAGAT TTGGGCAAAA TTATGGATCA CGTGAAGAAA 
GAAAAGAGAG AAAAGGAACA GCAGGAAGAG AAGTCTGGTT TGTTCAGGAA CATGGGGAGG 
AATGAAGATG GTGAAAGAAG AGCTATGATA ACTCCTGCTC TCCGAGAAGC CCTTACTAAA 
CAAGTCGATG CTCCGAGGGA GAGGAGCTTG TTACAAACAC ACATTCTATG GAATGAGAGC 
CATCGCTGCA TGGAAACCAC CCCGAGCTTG GCGTGTGCTA ATAAATGTGT CTTCTGTTGG 
TGGCACCACA ACAACCCTGT GGGCACTGAA TGGTTGTGGA AGATGGACCA GCCTGAAATG 
ATCTTGAAGG AAGCCATTGA AAACCATCAG AACATGATTA AGCAGTTTAA AGGAGTACCG 
GGCGTCAAAG CAGAACGCTT TGAAGAAGGA ATGACGGTAA AGCACTGTGC ATTGTCCCTC 
GTGGGAGAAC CAATAATGTA CCCAGAGATC AACAGGTTTT TGAAGCTACT CCACCAGTGT 
AAAATCTCCA GCTTCCTGGT CACAAATGCA CAATTTCCTG CGGAAATCAG GAACCTCGAG 
CCAGTTACTC AGCTGTATGT CAGTGTGGAT GCCAGTACCA AAGACAGCCT GAAGAAAATC 
GACCGCCCAC TCTTCAAGGA TTTCTGGCAG CAATTCCTTG ACAGTTTAAA AGCCTTGGCA 
GTCAAGCAAC AACGAACTGT CTACAGACTG ATGCTCGTGA AAGCATGGAA CGTGGACGAG 
CTCCAGGCCT ACGCGCAGCT CGTGTCCCTG GGGAATCCTG ACTTCATCGA AGTGAAGGGC 
GTTACCTACT GCAGAGAAAG TTCAGCAAGC AGTCTTACCA TGGCCCATGT GCCCTGGCAT 
GAGGAAGTGG TACAGTTTGT CCGCGAGCTG GTGGATCTGA TCCCCGAATA TGAAATTGCA 
TGTGAACACG AACACTCTAA TTGCCTCCTG ATAGCACACA GAAAGTTTAA AATTGGTGGT 
GAATGGTGGA CATGGATCGA TTATAACCGC TTCCAGGAGC TCATCCAGGA ATATGAAGAT 
AGTGGTGGAT CAAAAACGTT CAGCGCAAAG GATTATATGG CCAGAACTCC TCACTGGGCA 
TTATTTGGTG CCAATGAAAG AAGCTTTGAT CCCAAGGACA CAAGACATCA GAGAAAGAAC 
AAATCAAAGG CTATTTCTGG ATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TYW1B ( XM_017012221.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867710.1 Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X2, mRNA.

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CloneID	OHu97261
Clone ID Related Accession (Same CDS sequence)	XM_017012221.1
Accession Version	XM_017012221.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTT CTGCGGATAC ATGGGACCTC TCCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATCTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCGAGA TGCAGGGATT TGCAACAGTT CTTGCTGAAG CAGTTACGTC CCTGGATCTG 
CCTGTGGCCA TTATTAATCT AAAAGAATAT GATCCAGATG ATCATCTGAT AGAAGAGGTG 
ACTAGTAAAA ATGTCTGTGT CTTCCTGGTT GCGACATACA CTGACGGCCT ACCAACCGAA 
AGTGCAGAGT GGTTCTGCAA ATGGTTAGAG GAAGCATCCA TTGATTTTCG ATTTGGCAAA 
ACTTACCTGA AGGGTATGAG AGATGCGGTA TTTGGCCTGG GAAATTCTGC CTATGCTAGC 
CACTTCAACA AGGTTGGCAA AAATGTTGAC AAGTGGCTCT GGATGCTTGG CGTGCATCGT 
GTGATGAGTC GAGGGGAGGG CGACTGCGAC GTGGTTAAAA GCAAGCACGG CAGCATTGAG 
GCCAACTTCA GAGCATGGAA GACCAAGTTC ATCTCCCAGC TGCAGGCACT TCAGAAAGGG 
GAGAGAAAGA AGTCCTGTGG CGGCCACTGC AAGAAAGGCA AATGTGAATC TCACCAACAT 
GGCTCAGAGG AGAGGGAGGA AGGATCTCAA GAGCAGGACG AATTGCATCA CAGAGACACC 
AAGGAGGAAG AACCCTTCGA GAGCTCCAGT GAAGAAGAGT TTGGTGGTGA GGACCATCAG 
AGCCTAAATT CCATTGTTGA TGTTGAAGAT TTGGGCAAAA TTATGGATCA CGTGAAGAAA 
GAAAAGAGAG AAAAGGAACA GCAGGAAGAG AAGTCTGGTT TGTTCAGGAA CATGGGGAGG 
AATGAAGATG GTGAAAGAAG AGCTATGATA ACTCCTGCTC TCCGAGAAGC CCTTACTAAA 
CAAGGAGTAC CGGGCGTCAA AGCAGAACGC TTTGAAGAAG GAATGACGGT AAAGCACTGT 
GCATTGTCCC TCGTGGGAGA ACCAATAATG TACCCAGAGA TCAACAGGTT TTTGAAGCTA 
CTCCACCAGT GTAAAATCTC CAGCTTCCTG GTCACAAATG CACAATTTCC TGCGGAAATC 
AGGAACCTCG AGCCAGTTAC TCAGCTGTAT GTCAGTGTGG ATGCCAGTAC CAAAGACAGC 
CTGAAGAAAA TCGACCGCCC ACTCTTCAAG GATTTCTGGC AGCAATTCCT TGACAGTTTA 
AAAGCCTTGG CAGTCAAGCA ACAACGAACT GTCTACAGAC TGATGCTCGT GAAAGCATGG 
AACGTGGACG AGCTCCAGGC CTACGCGCAG CTCGTGTCCC TGGGGAATCC TGACTTCATC 
GAAGTGAAGG GCGTTACCTA CTGCAGAGAA AGTTCAGCAA GCAGTCTTAC CATGGCCCAT 
GTGCCCTGGC ATGAGGAAGT GGTACAGTTT GTCCGCGAGC TGGTGGATCT GATCCCCGAA 
TATGAAATTG CATGTGAACA CGAACACTCT AATTGCCTCC TGATAGCACA CAGAAAGTTT 
AAAATTGGTG GTGAATGGTG GACATGGATC GATTATAACC GCTTCCAGGA GCTCATCCAG 
GAATATGAAG ATAGTGGTGG ATCAAAAACG TTCAGCGCAA AGGATTATAT GGCCAGAACT 
CCTCACTGGG CATTATTTGG TGCCAATGAA AGAAGCTTTG ATCCCAAGGA CACAAGACAT 
CAGAGAAAGA ACAAATCAAA GGCTATTTCT GGATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867710.1
CDS	160..1938
Translation	MDPSADTWDLSSPLISLWINRFYIYLGFAVSISLWICVQIVIEMQGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRDAVFGLGNSAYASHFNKVGKNVDKWLWMLGVHRVMSRGEGDCDVVKSKHGSIEANFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSQEQDELHHRDTKEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQQFLDSLKALAVKQQRTVYRLMLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCRESSASSLTMAHVPWHEEVVQFVRELVDLIPEYEIACEHEHSNCLLIAHRKFKIGGEWWTWIDYNRFQELIQEYEDSGGSKTFSAKDYMARTPHWALFGANERSFDPKDTRHQRKNKSKAISGC

Target ORF information:

RefSeq Version	XM_017012221.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012221.1

ORF Insert Sequence:

ATGGATCCTT CTGCGGATAC ATGGGACCTC TCCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATCTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCGAGA TGCAGGGATT TGCAACAGTT CTTGCTGAAG CAGTTACGTC CCTGGATCTG 
CCTGTGGCCA TTATTAATCT AAAAGAATAT GATCCAGATG ATCATCTGAT AGAAGAGGTG 
ACTAGTAAAA ATGTCTGTGT CTTCCTGGTT GCGACATACA CTGACGGCCT ACCAACCGAA 
AGTGCAGAGT GGTTCTGCAA ATGGTTAGAG GAAGCATCCA TTGATTTTCG ATTTGGCAAA 
ACTTACCTGA AGGGTATGAG AGATGCGGTA TTTGGCCTGG GAAATTCTGC CTATGCTAGC 
CACTTCAACA AGGTTGGCAA AAATGTTGAC AAGTGGCTCT GGATGCTTGG CGTGCATCGT 
GTGATGAGTC GAGGGGAGGG CGACTGCGAC GTGGTTAAAA GCAAGCACGG CAGCATTGAG 
GCCAACTTCA GAGCATGGAA GACCAAGTTC ATCTCCCAGC TGCAGGCACT TCAGAAAGGG 
GAGAGAAAGA AGTCCTGTGG CGGCCACTGC AAGAAAGGCA AATGTGAATC TCACCAACAT 
GGCTCAGAGG AGAGGGAGGA AGGATCTCAA GAGCAGGACG AATTGCATCA CAGAGACACC 
AAGGAGGAAG AACCCTTCGA GAGCTCCAGT GAAGAAGAGT TTGGTGGTGA GGACCATCAG 
AGCCTAAATT CCATTGTTGA TGTTGAAGAT TTGGGCAAAA TTATGGATCA CGTGAAGAAA 
GAAAAGAGAG AAAAGGAACA GCAGGAAGAG AAGTCTGGTT TGTTCAGGAA CATGGGGAGG 
AATGAAGATG GTGAAAGAAG AGCTATGATA ACTCCTGCTC TCCGAGAAGC CCTTACTAAA 
CAAGGAGTAC CGGGCGTCAA AGCAGAACGC TTTGAAGAAG GAATGACGGT AAAGCACTGT 
GCATTGTCCC TCGTGGGAGA ACCAATAATG TACCCAGAGA TCAACAGGTT TTTGAAGCTA 
CTCCACCAGT GTAAAATCTC CAGCTTCCTG GTCACAAATG CACAATTTCC TGCGGAAATC 
AGGAACCTCG AGCCAGTTAC TCAGCTGTAT GTCAGTGTGG ATGCCAGTAC CAAAGACAGC 
CTGAAGAAAA TCGACCGCCC ACTCTTCAAG GATTTCTGGC AGCAATTCCT TGACAGTTTA 
AAAGCCTTGG CAGTCAAGCA ACAACGAACT GTCTACAGAC TGATGCTCGT GAAAGCATGG 
AACGTGGACG AGCTCCAGGC CTACGCGCAG CTCGTGTCCC TGGGGAATCC TGACTTCATC 
GAAGTGAAGG GCGTTACCTA CTGCAGAGAA AGTTCAGCAA GCAGTCTTAC CATGGCCCAT 
GTGCCCTGGC ATGAGGAAGT GGTACAGTTT GTCCGCGAGC TGGTGGATCT GATCCCCGAA 
TATGAAATTG CATGTGAACA CGAACACTCT AATTGCCTCC TGATAGCACA CAGAAAGTTT 
AAAATTGGTG GTGAATGGTG GACATGGATC GATTATAACC GCTTCCAGGA GCTCATCCAG 
GAATATGAAG ATAGTGGTGG ATCAAAAACG TTCAGCGCAA AGGATTATAT GGCCAGAACT 
CCTCACTGGG CATTATTTGG TGCCAATGAA AGAAGCTTTG ATCCCAAGGA CACAAGACAT 
CAGAGAAAGA ACAAATCAAA GGCTATTTCT GGATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Discovery and refinement of loci associated with lipid levels.
Nature genetics45(11)1274-1283(2013 Nov)
Willer CJ,Schmidt EM,Sengupta S,Peloso GM,Gustafsson S,Kanoni S,Ganna A,Chen J,Buchkovich ML,Mora S,Beckmann JS,Bragg-Gresham JL,Chang HY,Demirkan A,Den Hertog HM,Do R,Donnelly LA,Ehret GB,Esko T,Feitosa MF,Ferreira T,Fischer K,Fontanillas P,Fraser RM,Freitag DF,Gurdasani D,Heikkil? K,Hypp?nen E,Isaacs A,Jackson AU,Johansson ?,Johnson T,Kaakinen M,Kettunen J,Kleber ME,Li X,Luan J,Lyytik?inen LP,Magnusson PKE,Mangino M,Mihailov E,Montasser ME,M?ller-Nurasyid M,Nolte IM,O'Connell JR,Palmer CD,Perola M,Petersen AK,Sanna S,Saxena R,Service SK,Shah S,Shungin D,Sidore C,Song C,Strawbridge RJ,Surakka I,Tanaka T,Teslovich TM,Thorleifsson G,Van den Herik EG,Voight BF,Volcik KA,Waite LL,Wong A,Wu Y,Zhang W,Absher D,Asiki G,Barroso I,Been LF,Bolton JL,Bonnycastle LL,Brambilla P,Burnett MS,Cesana G,Dimitriou M,Doney ASF,D?ring A,Elliott P,Epstein SE,Ingi Eyjolfsson G,Gigante B,Goodarzi MO,Grallert H,Gravito ML,Groves CJ,Hallmans G,Hartikainen AL,Hayward C,Hernandez D,Hicks AA,Holm H,Hung YJ,Illig T,Jones MR,Kaleebu P,Kastelein JJP,Khaw KT,Kim E,Klopp N,Komulainen P,Kumari M,Langenberg C,Lehtim?ki T,Lin SY,Lindstr?m J,Loos RJF,Mach F,McArdle WL,Meisinger C,Mitchell BD,M?ller G,Nagaraja R,Narisu N,Nieminen TVM,Nsubuga RN,Olafsson I,Ong KK,Palotie A,Papamarkou T,Pomilla C,Pouta A,Rader DJ,Reilly MP,Ridker PM,Rivadeneira F,Rudan I,Ruokonen A,Samani N,Scharnagl H,Seeley J,Silander K,Stan??kov? A,Stirrups K,Swift AJ,Tiret L,Uitterlinden AG,van Pelt LJ,Vedantam S,Wainwright N,Wijmenga C,Wild SH,Willemsen G,Wilsgaard T,Wilson JF,Young EH,Zhao JH,Adair LS,Arveiler D,Assimes TL,Bandinelli S,Bennett F,Bochud M,Boehm BO,Boomsma DI,Borecki IB,Bornstein SR,Bovet P,Burnier M,Campbell H,Chakravarti A,Chambers JC,Chen YI,Collins FS,Cooper RS,Danesh J,Dedoussis G,de Faire U,Feranil AB,Ferri?res J,Ferrucci L,Freimer NB,Gieger C,Groop LC,Gudnason V,Gyllensten U,Hamsten A,Harris TB,Hingorani A,Hirschhorn JN,Hofman A,Hovingh GK,Hsiung CA,Humphries SE,Hunt SC,Hveem K,Iribarren C,J?rvelin MR,Jula A,K?h?nen M,Kaprio J,Kes?niemi A,Kivimaki M,Kooner JS,Koudstaal PJ,Krauss RM,Kuh D,Kuusisto J,Kyvik KO,Laakso M,Lakka TA,Lind L,Lindgren CM,Martin NG,M?rz W,McCarthy MI,McKenzie CA,Meneton P,Metspalu A,Moilanen L,Morris AD,Munroe PB,Nj?lstad I,Pedersen NL,Power C,Pramstaller PP,Price JF,Psaty BM,Quertermous T,Rauramaa R,Saleheen D,Salomaa V,Sanghera DK,Saramies J,Schwarz PEH,Sheu WH,Shuldiner AR,Siegbahn A,Spector TD,Stefansson K,Strachan DP,Tayo BO,Tremoli E,Tuomilehto J,Uusitupa M,van Duijn CM,Vollenweider P,Wallentin L,Wareham NJ,Whitfield JB,Wolffenbuttel BHR,Ordovas JM,Boerwinkle E,Palmer CNA,Thorsteinsdottir U,Chasman DI,Rotter JI,Franks PW,Ripatti S,Cupples LA,Sandhu MS,Rich SS,Boehnke M,Deloukas P,Kathiresan S,Mohlke KL,Ingelsson E,Abecasis GR,

Biological, clinical and population relevance of 95 loci for blood lipids.
Nature466(7307)707-13(2010 Aug)
Teslovich TM,Musunuru K,Smith AV,Edmondson AC,Stylianou IM,Koseki M,Pirruccello JP,Ripatti S,Chasman DI,Willer CJ,Johansen CT,Fouchier SW,Isaacs A,Peloso GM,Barbalic M,Ricketts SL,Bis JC,Aulchenko YS,Thorleifsson G,Feitosa MF,Chambers J,Orho-Melander M,Melander O,Johnson T,Li X,Guo X,Li M,Shin Cho Y,Jin Go M,Jin Kim Y,Lee JY,Park T,Kim K,Sim X,Twee-Hee Ong R,Croteau-Chonka DC,Lange LA,Smith JD,Song K,Hua Zhao J,Yuan X,Luan J,Lamina C,Ziegler A,Zhang W,Zee RY,Wright AF,Witteman JC,Wilson JF,Willemsen G,Wichmann HE,Whitfield JB,Waterworth DM,Wareham NJ,Waeber G,Vollenweider P,Voight BF,Vitart V,Uitterlinden AG,Uda M,Tuomilehto J,Thompson JR,Tanaka T,Surakka I,Stringham HM,Spector TD,Soranzo N,Smit JH,Sinisalo J,Silander K,Sijbrands EJ,Scuteri A,Scott J,Schlessinger D,Sanna S,Salomaa V,Saharinen J,Sabatti C,Ruokonen A,Rudan I,Rose LM,Roberts R,Rieder M,Psaty BM,Pramstaller PP,Pichler I,Perola M,Penninx BW,Pedersen NL,Pattaro C,Parker AN,Pare G,Oostra BA,O'Donnell CJ,Nieminen MS,Nickerson DA,Montgomery GW,Meitinger T,McPherson R,McCarthy MI,McArdle W,Masson D,Martin NG,Marroni F,Mangino M,Magnusson PK,Lucas G,Luben R,Loos RJ,Lokki ML,Lettre G,Langenberg C,Launer LJ,Lakatta EG,Laaksonen R,Kyvik KO,Kronenberg F,K?nig IR,Khaw KT,Kaprio J,Kaplan LM,Johansson A,Jarvelin MR,Janssens AC,Ingelsson E,Igl W,Kees Hovingh G,Hottenga JJ,Hofman A,Hicks AA,Hengstenberg C,Heid IM,Hayward C,Havulinna AS,Hastie ND,Harris TB,Haritunians T,Hall AS,Gyllensten U,Guiducci C,Groop LC,Gonzalez E,Gieger C,Freimer NB,Ferrucci L,Erdmann J,Elliott P,Ejebe KG,D?ring A,Dominiczak AF,Demissie S,Deloukas P,de Geus EJ,de Faire U,Crawford G,Collins FS,Chen YD,Caulfield MJ,Campbell H,Burtt NP,Bonnycastle LL,Boomsma DI,Boekholdt SM,Bergman RN,Barroso I,Bandinelli S,Ballantyne CM,Assimes TL,Quertermous T,Altshuler D,Seielstad M,Wong TY,Tai ES,Feranil AB,Kuzawa CW,Adair LS,Taylor HA Jr,Borecki IB,Gabriel SB,Wilson JG,Holm H,Thorsteinsdottir U,Gudnason V,Krauss RM,Mohlke KL,Ordovas JM,Munroe PB,Kooner JS,Tall AR,Hegele RA,Kastelein JJ,Schadt EE,Rotter JI,Boerwinkle E,Strachan DP,Mooser V,Stefansson K,Reilly MP,Samani NJ,Schunkert H,Cupples LA,Sandhu MS,Ridker PM,Rader DJ,van Duijn CM,Peltonen L,Abecasis GR,Boehnke M,Kathiresan S

Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis.
Nucleic acids symposium series (2004)(50)65-6(2006)
Noma A,Suzuki T

Discovery of a gene family critical to wyosine base formation in a subset of phenylalanine-specific transfer RNAs.
The Journal of biological chemistry280(45)37616-22(2005 Nov)
Waas WF,de Cr?cy-Lagard V,Schimmel P

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TYW1B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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オンラインフォーム