ALKAL1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ALKAL1
Entrez Gene ID	389658
Full Name	ALK and LTK ligand 1
Synonyms	AUGA,AUGB,FAM150A,UNQ9433
General protein information	Preferred Names ALK and LTK ligand 1 Names ALK and LTK ligand 1 AUG-beta RPLK9433 augmentor beta augmentor-alpha family with sequence similarity 150 member A protein FAM150A
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8q11.23

mRNA and Protein(s)

mRNA	Protein	Name
NM_207413.3	NP_997296.1	ALK and LTK ligand 1 precursor
NM_207413.4	NP_997296.1	ALK and LTK ligand 1 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Fam150a	NP_001182661.1
Homo sapiens (human)	FAM150A	NP_997296.1
Bos taurus (cattle)	FAM150A	XP_002692709.1
Rattus norvegicus (Norway rat)	LOC100910984	XP_006237858.1
Pan troglodytes (chimpanzee)	FAM150A	XP_001149038.1
Canis lupus familiaris (dog)	LOC608857	XP_851088.2
	FAM150A	XP_001109192.1

Gene Ontology
Bibliography

Related articles in PubMed

ALKALs are in vivo ligands for ALK family receptor tyrosine kinases in the neural crest and derived cells.
Fadeev A, Mendoza-Garcia P, Irion U, Guan J, Pfeifer K, Wiessner S, Serluca F, Singh AP, N?sslein-Volhard C, Palmer RH
Proceedings of the National Academy of Sciences of the United States of America(2018 Jan)

Augmentor ? and ? (FAM150) are ligands of the receptor tyrosine kinases ALK and LTK: Hierarchy and specificity of ligand-receptor interactions.
Reshetnyak AV, Murray PB, Shi X, Mo ES, Mohanty J, Tome F, Bai H, Gunel M, Lax I, Schlessinger J
Proceedings of the National Academy of Sciences of the United States of America112(52)15862-7(2015 Dec)

FAM150A and FAM150B are activating ligands for anaplastic lymphoma kinase.
Guan J, Umapathy G, Yamazaki Y, Wolfstetter G, Mendoza P, Pfeifer K, Mohammed A, Hugosson F, Zhang H, Hsu AW, Halenbeck R, Hallberg B, Palmer RH
eLife4e09811(2015 Sep)

Deorphanization of the human leukocyte tyrosine kinase (LTK) receptor by a signaling screen of the extracellular proteome.
Zhang H, Pao LI, Zhou A, Brace AD, Halenbeck R, Hsu AW, Bray TL, Hestir K, Bosch E, Lee E, Wang G, Liu H, Wong BR, Kavanaugh WM, Williams LT
Proceedings of the National Academy of Sciences of the United States of America111(44)15741-5(2014 Nov)

The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.
Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C, Currell B, Deuel B, Dowd P, Eaton D, Foster J, Grimaldi C, Gu Q, Hass PE, Heldens S, Huang A, Kim HS, Klimowski L, Jin Y, Johnson S, Lee J, Lewis L, Liao D, Mark M, Robbie E, Sanchez C, Schoenfeld J, Seshagiri S, Simmons L, Singh J, Smith V, Stinson J, Vagts A, Vandlen R, Watanabe C, Wieand D, Woods K, Xie MH, Yansura D, Yi S, Yu G, Yuan J, Zhang M, Zhang Z, Goddard A, Wood WI, Godowski P, Gray A
Genome research13(10)2265-70(2003 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that leukocyte tyrosine kinase (LTK) ligand FAM150A (augmentor-beta; AUG-beta) is specific for LTK and only weakly binds to anaplastic lymphoma kinase (ALK).
Title: Augmentor ? and ? (FAM150) are ligands of the receptor tyrosine kinases ALK and LTK: Hierarchy and specificity of ligand-receptor interactions.

In conclusion, these data show that ALK is robustly activated by the FAM150A/B ligands.
Title: FAM150A and FAM150B are activating ligands for anaplastic lymphoma kinase.

Only two related secreted factors, FAM150A and FAM150B (family with sequence similarity 150 member A and member B), stimulated LTK phosphorylation.
Title: Deorphanization of the human leukocyte tyrosine kinase (LTK) receptor by a signaling screen of the extracellular proteome.

The following ALKAL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALKAL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03146	NM_207413.4 Latest version!	Homo sapiens ALK and LTK ligand 1 (ALKAL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu03146	NM_207413.3	Homo sapiens ALK and LTK ligand 1 (ALKAL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ALKAL1 ( NM_207413.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_997296.1 Homo sapiens ALK and LTK ligand 1 (ALKAL1), mRNA.

Price & Availability↑

CloneID	OHu03146
Clone ID Related Accession (Same CDS sequence)	NM_207413.3 , NM_207413.4
Accession Version	NM_207413.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	ALK and LTK ligand 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP423106.1, AY358213.1, AI858920.1 and AC013650.8. On Oct 10, 2008 this sequence version replaced NM_207413.2. ##Evidence-Data-START## Transcript exon combination :: ERR279857.251.1, AY358213.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCCCC TTAAGCCCGG CGCCCCTTTG CCCGCACTCT TCCTGCTGGC GCTGGCTTTG 
TCCCCGCACG GAGCCCACGG GAGGCCCCGG GGGCGCAGGG GAGCGCGCGT CACGGATAAG 
GAGCCCAAGC CGTTGCTTTT CCTCCCCGCG GCCGGGGCCG GCCGGACTCC CAGCGGCTCC 
CGGAGCGCAG AAATATTCCC AAGAGACTCT AACTTAAAAG ACAAATTCAT AAAGCATTTC 
ACAGGGCCGG TCACATTTTC ACCAGAATGC AGCAAACATT TCCACCGACT CTATTACAAT 
ACCAGGGAGT GCTCAACGCC AGCTTATTAC AAAAGATGTG CTAGATTGTT AACAAGATTA 
GCAGTGAGTC CACTGTGCTC CCAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997296.1
CDS	206..595
Translation	MRPLKPGAPLPALFLLALALSPHGAHGRPRGRRGARVTDKEPKPLLFLPAAGAGRTPSGSRSAEIFPRDSNLKDKFIKHFTGPVTFSPECSKHFHRLYYNTRECSTPAYYKRCARLLTRLAVSPLCSQT

Target ORF information:

RefSeq Version	NM_207413.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ALK and LTK ligand 1 (ALKAL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207413.3

ORF Insert Sequence:

ATGCGGCCCC TTAAGCCCGG CGCCCCTTTG CCCGCACTCT TCCTGCTGGC GCTGGCTTTG 
TCCCCGCACG GAGCCCACGG GAGGCCCCGG GGGCGCAGGG GAGCGCGCGT CACGGATAAG 
GAGCCCAAGC CGTTGCTTTT CCTCCCCGCG GCCGGGGCCG GCCGGACTCC CAGCGGCTCC 
CGGAGCGCAG AAATATTCCC AAGAGACTCT AACTTAAAAG ACAAATTCAT AAAGCATTTC 
ACAGGGCCGG TCACATTTTC ACCAGAATGC AGCAAACATT TCCACCGACT CTATTACAAT 
ACCAGGGAGT GCTCAACGCC AGCTTATTAC AAAAGATGTG CTAGATTGTT AACAAGATTA 
GCAGTGAGTC CACTGTGCTC CCAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALKAL1 ( NM_207413.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_997296.1 Homo sapiens ALK and LTK ligand 1 (ALKAL1), mRNA.

Price & Availability↑

CloneID	OHu03146
Clone ID Related Accession (Same CDS sequence)	NM_207413.3 , NM_207413.4
Accession Version	NM_207413.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	ALK and LTK ligand 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP423106.1, AY358213.1, AI858920.1 and AC013650.8. On Nov 22, 2018 this sequence version replaced NM_207413.3. ##Evidence-Data-START## Transcript exon combination :: ERR279857.251.1, AY358213.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000358543.9/ ENSP00000351345.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGGCCCC TTAAGCCCGG CGCCCCTTTG CCCGCACTCT TCCTGCTGGC GCTGGCTTTG 
TCCCCGCACG GAGCCCACGG GAGGCCCCGG GGGCGCAGGG GAGCGCGCGT CACGGATAAG 
GAGCCCAAGC CGTTGCTTTT CCTCCCCGCG GCCGGGGCCG GCCGGACTCC CAGCGGCTCC 
CGGAGCGCAG AAATATTCCC AAGAGACTCT AACTTAAAAG ACAAATTCAT AAAGCATTTC 
ACAGGGCCGG TCACATTTTC ACCAGAATGC AGCAAACATT TCCACCGACT CTATTACAAT 
ACCAGGGAGT GCTCAACGCC AGCTTATTAC AAAAGATGTG CTAGATTGTT AACAAGATTA 
GCAGTGAGTC CACTGTGCTC CCAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997296.1
CDS	175..564
Translation	MRPLKPGAPLPALFLLALALSPHGAHGRPRGRRGARVTDKEPKPLLFLPAAGAGRTPSGSRSAEIFPRDSNLKDKFIKHFTGPVTFSPECSKHFHRLYYNTRECSTPAYYKRCARLLTRLAVSPLCSQT

Target ORF information:

RefSeq Version	NM_207413.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ALK and LTK ligand 1 (ALKAL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207413.4

ORF Insert Sequence:

ATGCGGCCCC TTAAGCCCGG CGCCCCTTTG CCCGCACTCT TCCTGCTGGC GCTGGCTTTG 
TCCCCGCACG GAGCCCACGG GAGGCCCCGG GGGCGCAGGG GAGCGCGCGT CACGGATAAG 
GAGCCCAAGC CGTTGCTTTT CCTCCCCGCG GCCGGGGCCG GCCGGACTCC CAGCGGCTCC 
CGGAGCGCAG AAATATTCCC AAGAGACTCT AACTTAAAAG ACAAATTCAT AAAGCATTTC 
ACAGGGCCGG TCACATTTTC ACCAGAATGC AGCAAACATT TCCACCGACT CTATTACAAT 
ACCAGGGAGT GCTCAACGCC AGCTTATTAC AAAAGATGTG CTAGATTGTT AACAAGATTA 
GCAGTGAGTC CACTGTGCTC CCAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ALKALs are in vivo ligands for ALK family receptor tyrosine kinases in the neural crest and derived cells.
Proceedings of the National Academy of Sciences of the United States of America(2018 Jan)
Fadeev A,Mendoza-Garcia P,Irion U,Guan J,Pfeifer K,Wiessner S,Serluca F,Singh AP,N?sslein-Volhard C,Palmer RH

Augmentor ? and ? (FAM150) are ligands of the receptor tyrosine kinases ALK and LTK: Hierarchy and specificity of ligand-receptor interactions.
Proceedings of the National Academy of Sciences of the United States of America112(52)15862-7(2015 Dec)
Reshetnyak AV,Murray PB,Shi X,Mo ES,Mohanty J,Tome F,Bai H,Gunel M,Lax I,Schlessinger J

FAM150A and FAM150B are activating ligands for anaplastic lymphoma kinase.
eLife4e09811(2015 Sep)
Guan J,Umapathy G,Yamazaki Y,Wolfstetter G,Mendoza P,Pfeifer K,Mohammed A,Hugosson F,Zhang H,Hsu AW,Halenbeck R,Hallberg B,Palmer RH

Deorphanization of the human leukocyte tyrosine kinase (LTK) receptor by a signaling screen of the extracellular proteome.
Proceedings of the National Academy of Sciences of the United States of America111(44)15741-5(2014 Nov)
Zhang H,Pao LI,Zhou A,Brace AD,Halenbeck R,Hsu AW,Bray TL,Hestir K,Bosch E,Lee E,Wang G,Liu H,Wong BR,Kavanaugh WM,Williams LT

The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.
Genome research13(10)2265-70(2003 Oct)
Clark HF,Gurney AL,Abaya E,Baker K,Baldwin D,Brush J,Chen J,Chow B,Chui C,Crowley C,Currell B,Deuel B,Dowd P,Eaton D,Foster J,Grimaldi C,Gu Q,Hass PE,Heldens S,Huang A,Kim HS,Klimowski L,Jin Y,Johnson S,Lee J,Lewis L,Liao D,Mark M,Robbie E,Sanchez C,Schoenfeld J,Seshagiri S,Simmons L,Singh J,Smith V,Stinson J,Vagts A,Vandlen R,Watanabe C,Wieand D,Woods K,Xie MH,Yansura D,Yi S,Yu G,Yuan J,Zhang M,Zhang Z,Goddard A,Wood WI,Godowski P,Gray A

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ALKAL1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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