DPPA3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DPPA3
Entrez Gene ID	359787
Full Name	developmental pluripotency associated 3
Synonyms	Pgc7,STELLA
General protein information	Preferred Names developmental pluripotency associated 3 Names developmental pluripotency-associated protein 3 stella-related protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12p13.31
Summary	This gene encodes a protein that in mice may function as a maternal factor during the preimplantation stage of development. In mice, this gene may play a role in transcriptional repression, cell division, and maintenance of cell pluripotentiality. In humans, related intronless loci are located on chromosomes 14 and X. [provided by RefSeq, Jul 2008].
Disorder MIM:	608408

mRNA and Protein(s)

mRNA	Protein	Name
NM_199286.3	NP_954980.1	developmental pluripotency-associated protein 3
NM_199286.4	NP_954980.1	developmental pluripotency-associated protein 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	DPPA3	NP_954980.1
Pan troglodytes (chimpanzee)	DPPA3	XP_001164778.1

Gene Ontology
Bibliography

Related articles in PubMed

Pluripotency induction in HEK293T cells by concurrent expression of STELLA, OCT4 and NANOS2.
Farshchian M, Matin MM, Bahrami M, Geerts D, Dastpak M, Shahriyari M, Tajeran M, Mirahmadi M, Bahrami AR
Biochemical and biophysical research communications480(4)635-640(2016 Nov)

Super-Enhancers at the Nanog Locus Differentially Regulate Neighboring Pluripotency-Associated Genes.
Blinka S, Reimer MH Jr, Pulakanti K, Rao S
Cell reports17(1)19-28(2016 09)

Global DNA hypomethylation coupled to cellular transformation and metastatic ability.
Funaki S, Nakamura T, Nakatani T, Umehara H, Nakashima H, Okumura M, Oboki K, Matsumoto K, Saito H, Nakano T
FEBS letters589(24 Pt B)4053-60(2015 Dec)

Inhibition of maintenance DNA methylation by Stella.
Funaki S, Nakamura T, Nakatani T, Umehara H, Nakashima H, Nakano T
Biochemical and biophysical research communications453(3)455-60(2014 Oct)

STELLA facilitates differentiation of germ cell and endodermal lineages of human embryonic stem cells.
Wongtrakoongate P, Jones M, Gokhale PJ, Andrews PW
PloS one8(2)e56893(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We conclude that heterogeneous population of the HEK293T cells might be easily shifted towards expression of the pluripotency markers by ectopic expression of the SON factors or by growth in serum depleted media.
Title: Pluripotency induction in HEK293T cells by concurrent expression of STELLA, OCT4 and NANOS2.

Super-enhancers at the Nanog locus differentially regulate neighboring pluripotency-associated genes, in particular, DPPA3.
Title: Super-Enhancers at the Nanog Locus Differentially Regulate Neighboring Pluripotency-Associated Genes.

Data suggest enforced expression of Stella into normal cells induces not only global DNA demethylation but also neoplastic transformation and increases metastatic ability; these changes involve altered regulation of neoplastic gene expression.
Title: Global DNA hypomethylation coupled to cellular transformation and metastatic ability.

Considering that impaired DNA methylation profiles are associated with various developmental or disease phenomena, Stella may be a powerful tool with which to study the biological effects of global DNA hypomethylation
Title: Inhibition of maintenance DNA methylation by Stella.

a role for STELLA in facilitating germline and endodermal differentiation of human ES cells
Title: STELLA facilitates differentiation of germ cell and endodermal lineages of human embryonic stem cells.

The following DPPA3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DPPA3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu29823	NM_199286.4 Latest version!	Homo sapiens developmental pluripotency associated 3 (DPPA3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu29823	NM_199286.3	Homo sapiens developmental pluripotency associated 3 (DPPA3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DPPA3 ( NM_199286.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_954980.1 Homo sapiens developmental pluripotency associated 3 (DPPA3), mRNA.

Price & Availability↑

CloneID	OHu29823
Clone ID Related Accession (Same CDS sequence)	NM_199286.3 , NM_199286.4
Accession Version	NM_199286.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-19
Organism	Homo sapiens(human)
Product	developmental pluripotency-associated protein 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY317075.1 and AC006517.46. On Feb 12, 2015 this sequence version replaced NM_199286.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY317075.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142363 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCCAT CACAGTTTAA TCCAACCTAC ATCCCAGGGT CTCCACAAAT GCTCACCGAA 
GAAAATTCCC GGGACGATTC AGGGGCCTCT CAAATCTCCT CCGAGACGTT GATAAAGAAC 
CTTAGTAACT TGACTATCAA CGCTAGTAGC GAATCTGTTT CCCCTCTATC GGAAGCTTTA 
CTCCGTCGAG AGTCTGTAGG AGCAGCAGTC CTCAGGGAAA TCGAAGATGA GTGGCTTTAC 
AGCAGGAGAG GAGTAAGAAC ATTGCTGTCT GTGCAGAGAG AAAAGATGGC AAGATTGAGA 
TACATGTTAC TCGGCGGAGT TCGTACGCAT GAAAGAAGAC CAACAAACAA GGAGCCTAAG 
GGAGTTAAGA AGGAATCAAG ACCATTCAAA TGTCCCTGCA GTTTCTGCGT GTCTAATGGA 
TGGGATCCTT CTGAGAATGC TAGAATAGGG AATCAAGACA CCAAGCCACT TCAGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954980.1
CDS	118..597
Translation	MDPSQFNPTYIPGSPQMLTEENSRDDSGASQISSETLIKNLSNLTINASSESVSPLSEALLRRESVGAAVLREIEDEWLYSRRGVRTLLSVQREKMARLRYMLLGGVRTHERRPTNKEPKGVKKESRPFKCPCSFCVSNGWDPSENARIGNQDTKPLQP

Target ORF information:

RefSeq Version	NM_199286.3
Organism	Homo sapiens(human)
Definition	Homo sapiens developmental pluripotency associated 3 (DPPA3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199286.3

ORF Insert Sequence:

ATGGACCCAT CACAGTTTAA TCCAACCTAC ATCCCAGGGT CTCCACAAAT GCTCACCGAA 
GAAAATTCCC GGGACGATTC AGGGGCCTCT CAAATCTCCT CCGAGACGTT GATAAAGAAC 
CTTAGTAACT TGACTATCAA CGCTAGTAGC GAATCTGTTT CCCCTCTATC GGAAGCTTTA 
CTCCGTCGAG AGTCTGTAGG AGCAGCAGTC CTCAGGGAAA TCGAAGATGA GTGGCTTTAC 
AGCAGGAGAG GAGTAAGAAC ATTGCTGTCT GTGCAGAGAG AAAAGATGGC AAGATTGAGA 
TACATGTTAC TCGGCGGAGT TCGTACGCAT GAAAGAAGAC CAACAAACAA GGAGCCTAAG 
GGAGTTAAGA AGGAATCAAG ACCATTCAAA TGTCCCTGCA GTTTCTGCGT GTCTAATGGA 
TGGGATCCTT CTGAGAATGC TAGAATAGGG AATCAAGACA CCAAGCCACT TCAGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPPA3 ( NM_199286.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_954980.1 Homo sapiens developmental pluripotency associated 3 (DPPA3), mRNA.

Price & Availability↑

CloneID	OHu29823
Clone ID Related Accession (Same CDS sequence)	NM_199286.3 , NM_199286.4
Accession Version	NM_199286.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	developmental pluripotency-associated protein 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006517.46 and AY317075.1. On Nov 23, 2018 this sequence version replaced NM_199286.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY317075.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142363 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000345088.3/ ENSP00000339250.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGACCCAT CACAGTTTAA TCCAACCTAC ATCCCAGGGT CTCCACAAAT GCTCACCGAA 
GAAAATTCCC GGGACGATTC AGGGGCCTCT CAAATCTCCT CCGAGACGTT GATAAAGAAC 
CTTAGTAACT TGACTATCAA CGCTAGTAGC GAATCTGTTT CCCCTCTATC GGAAGCTTTA 
CTCCGTCGAG AGTCTGTAGG AGCAGCAGTC CTCAGGGAAA TCGAAGATGA GTGGCTTTAC 
AGCAGGAGAG GAGTAAGAAC ATTGCTGTCT GTGCAGAGAG AAAAGATGGC AAGATTGAGA 
TACATGTTAC TCGGCGGAGT TCGTACGCAT GAAAGAAGAC CAACAAACAA GGAGCCTAAG 
GGAGTTAAGA AGGAATCAAG ACCATTCAAA TGTCCCTGCA GTTTCTGCGT GTCTAATGGA 
TGGGATCCTT CTGAGAATGC TAGAATAGGG AATCAAGACA CCAAGCCACT TCAGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954980.1
CDS	139..618
Translation	MDPSQFNPTYIPGSPQMLTEENSRDDSGASQISSETLIKNLSNLTINASSESVSPLSEALLRRESVGAAVLREIEDEWLYSRRGVRTLLSVQREKMARLRYMLLGGVRTHERRPTNKEPKGVKKESRPFKCPCSFCVSNGWDPSENARIGNQDTKPLQP

Target ORF information:

RefSeq Version	NM_199286.4
Organism	Homo sapiens(human)
Definition	Homo sapiens developmental pluripotency associated 3 (DPPA3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199286.4

ORF Insert Sequence:

ATGGACCCAT CACAGTTTAA TCCAACCTAC ATCCCAGGGT CTCCACAAAT GCTCACCGAA 
GAAAATTCCC GGGACGATTC AGGGGCCTCT CAAATCTCCT CCGAGACGTT GATAAAGAAC 
CTTAGTAACT TGACTATCAA CGCTAGTAGC GAATCTGTTT CCCCTCTATC GGAAGCTTTA 
CTCCGTCGAG AGTCTGTAGG AGCAGCAGTC CTCAGGGAAA TCGAAGATGA GTGGCTTTAC 
AGCAGGAGAG GAGTAAGAAC ATTGCTGTCT GTGCAGAGAG AAAAGATGGC AAGATTGAGA 
TACATGTTAC TCGGCGGAGT TCGTACGCAT GAAAGAAGAC CAACAAACAA GGAGCCTAAG 
GGAGTTAAGA AGGAATCAAG ACCATTCAAA TGTCCCTGCA GTTTCTGCGT GTCTAATGGA 
TGGGATCCTT CTGAGAATGC TAGAATAGGG AATCAAGACA CCAAGCCACT TCAGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pluripotency induction in HEK293T cells by concurrent expression of STELLA, OCT4 and NANOS2.
Biochemical and biophysical research communications480(4)635-640(2016 Nov)
Farshchian M,Matin MM,Bahrami M,Geerts D,Dastpak M,Shahriyari M,Tajeran M,Mirahmadi M,Bahrami AR

Super-Enhancers at the Nanog Locus Differentially Regulate Neighboring Pluripotency-Associated Genes.
Cell reports17(1)19-28(2016 09)
Blinka S,Reimer MH Jr,Pulakanti K,Rao S

Global DNA hypomethylation coupled to cellular transformation and metastatic ability.
FEBS letters589(24 Pt B)4053-60(2015 Dec)
Funaki S,Nakamura T,Nakatani T,Umehara H,Nakashima H,Okumura M,Oboki K,Matsumoto K,Saito H,Nakano T

Inhibition of maintenance DNA methylation by Stella.
Biochemical and biophysical research communications453(3)455-60(2014 Oct)
Funaki S,Nakamura T,Nakatani T,Umehara H,Nakashima H,Nakano T

STELLA facilitates differentiation of germ cell and endodermal lineages of human embryonic stem cells.
PloS one8(2)e56893(2013)
Wongtrakoongate P,Jones M,Gokhale PJ,Andrews PW

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DPPA3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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