IBSP cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	IBSP
Entrez Gene ID	3381
Full Name	integrin binding sialoprotein
Synonyms	BNSP,BSP,BSP-II,SP-II
General protein information	Preferred Names integrin binding sialoprotein Names bone sialoprotein 2 BSP II bone sialoprotein II cell-binding sialoprotein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q22.1
Summary	The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. [provided by RefSeq, Jul 2008].
Disorder MIM:	147563

mRNA and Protein(s)

mRNA	Protein	Name
NM_004967.4	NP_004958.2	bone sialoprotein 2 precursor
NM_004967.3	NP_004958.2	bone sialoprotein 2 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ibsp	NP_032344.2
	IBSP	XP_001098825.1
Canis lupus familiaris (dog)	IBSP	XP_851449.1
Rattus norvegicus (Norway rat)	Ibsp	NP_036719.2
Pan troglodytes (chimpanzee)	IBSP	XP_526631.1
Bos taurus (cattle)	IBSP	NP_776509.1
Homo sapiens (human)	IBSP	NP_004958.2

Gene Ontology
Bibliography

Related articles in PubMed

Cancer Secretome May Influence BSP and DSP Expression in Human Salivary Gland Cells.
Hamilton SL, Ferando B, Eapen AS, Yu JC, Joy AR
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society65(3)139-151(2017 03)

Serum levels of PSA, ALP, ICTP, and BSP in prostate cancer patients and the significance of ROC curve in the diagnosis of prostate cancer bone metastases.
Wei RJ, Li TY, Yang XC, Jia N, Yang XL, Song HB
Genetics and molecular research : GMR15(2)(2016 Jun)

Preameloblast-Derived Factors Mediate Osteoblast Differentiation of Human Bone Marrow Mesenchymal Stem Cells by Runx2-Osterix-BSP Signaling.
Choung HW, Lee DS, Lee HK, Shon WJ, Park JC
Tissue engineering. Part A22(1-2)93-102(2016 Jan)

Endochondral ossification pathway genes and postmenopausal osteoporosis: Association and specific allele related serum bone sialoprotein levels in Han Chinese.
Zhang Y, Liu H, Zhang C, Zhang T, Zhang B, Li L, Chen G, Fu D, Wang K
Scientific reports516783(2015 Nov)

Correlation between calcification and bone sialoprotein and osteopontin in papillary thyroid carcinoma.
Wu G, Guo JJ, Ma ZY, Wang J, Zhou ZW, Wang Y
International journal of clinical and experimental pathology8(2)2010-7(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data indicate that secretome derived from salivary gland cancer cells can influence the expression of two potential biomarkers of oral cancer-namely, bone sialoprotein (BSP) and dentin sialoprotein (DSP)-in normal salivary gland cells.
Title: Cancer Secretome May Influence BSP and DSP Expression in Human Salivary Gland Cells.

In conclusion, serum levels of BSP, ALP, ICTP, and PSA increased in patients with bone metastases, and combined detection of all markers could improve the positive-predictive value.
Title: Serum levels of PSA, ALP, ICTP, and BSP in prostate cancer patients and the significance of ROC curve in the diagnosis of prostate cancer bone metastases.

Preameloblast-Derived Factors Mediate Osteoblast Differentiation of Human Bone Marrow Mesenchymal Stem Cells by Runx2-Osterix-BSP Signaling.
Title: Preameloblast-Derived Factors Mediate Osteoblast Differentiation of Human Bone Marrow Mesenchymal Stem Cells by Runx2-Osterix-BSP Signaling.

Two significant SNPs within IBSP, rs1054627 and rs17013181, were associated with BMD and postmenopausal osteoporosis by the two-stage strategy, and rs17013181 was also significantly associated with serum IBSP levels.
Title: Endochondral ossification pathway genes and postmenopausal osteoporosis: Association and specific allele related serum bone sialoprotein levels in Han Chinese.

the strong correlation between bone sialoprotein and OPN and papillary thyroid carcinoma suggests a role for BSP and OPN in calcification and tumor progression of papillary thyroid carcinoma
Title: Correlation between calcification and bone sialoprotein and osteopontin in papillary thyroid carcinoma.

The following IBSP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IBSP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu08955	NM_004967.4 Latest version!	Homo sapiens integrin binding sialoprotein (IBSP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
View Old Accession Versions >>
OHu08955	NM_004967.3	Homo sapiens integrin binding sialoprotein (IBSP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IBSP ( NM_004967.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004958.2 Homo sapiens integrin binding sialoprotein (IBSP), mRNA.

Price & Availability↑

CloneID	OHu08955
Clone ID Related Accession (Same CDS sequence)	NM_004967.4 , NM_004967.3
Accession Version	NM_004967.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	bone sialoprotein 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC093768.3, AI751848.1, J05213.1, DV462680.1, BQ573754.1 and AA452255.1. On Nov 22, 2018 this sequence version replaced NM_004967.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.193979.1, SRR1660807.136311.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2153307 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000226284.7/ ENSP00000226284.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAGACTG CTTTAATTTT GCTCAGCATT TTGGGAATGG CCTGTGCTTT CTCAATGAAA 
AATTTGCATC GAAGAGTCAA AATAGAGGAT TCTGAAGAAA ATGGGGTCTT TAAGTACAGG 
CCACGATATT ATCTTTACAA GCATGCCTAC TTTTATCCTC ATTTAAAACG ATTTCCAGTT 
CAGGGCAGTA GTGACTCATC CGAAGAAAAT GGAGATGACA GTTCAGAAGA GGAGGAGGAA 
GAAGAGGAGA CTTCAAATGA AGGAGAAAAC AATGAAGAAT CGAATGAAGA TGAAGACTCT 
GAGGCTGAGA ATACCACACT TTCTGCTACA ACACTGGGCT ATGGAGAGGA CGCCACGCCT 
GGCACAGGGT ATACAGGGTT AGCTGCAATC CAGCTTCCCA AGAAGGCTGG GGATATAACA 
AATAAAGCTA CAAAAGAGAA GGAAAGTGAT GAAGAAGAAG AGGAGGAAGA GGAAGGAAAT 
GAAAACGAAG AAAGCGAAGC AGAAGTGGAT GAAAACGAAC AAGGCATAAA CGGCACCAGT 
ACCAACAGCA CAGAGGCAGA AAACGGCAAC GGCAGCAGCG GAGGAGACAA TGGAGAAGAA 
GGGGAAGAAG AAAGTGTCAC TGGAGCCAAT GCAGAAGACA CCACAGAGAC CGGAAGGCAG 
GGCAAGGGCA CCTCGAAGAC AACAACCTCT CCAAATGGTG GGTTTGAACC TACAACCCCA 
CCACAAGTCT ATAGAACCAC TTCCCCACCT TTTGGGAAAA CCACCACCGT TGAATACGAG 
GGGGAGTACG AATACACGGG CGCCAATGAA TACGACAATG GATATGAAAT CTATGAAAGT 
GAGAACGGGG AACCTCGTGG GGACAATTAC CGAGCCTATG AAGATGAGTA CAGCTACTTT 
AAAGGACAAG GCTACGATGG CTATGATGGT CAGAATTACT ACCACCACCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004958.2
CDS	95..1048
Translation	MKTALILLSILGMACAFSMKNLHRRVKIEDSEENGVFKYRPRYYLYKHAYFYPHLKRFPVQGSSDSSEENGDDSSEEEEEEEETSNEGENNEESNEDEDSEAENTTLSATTLGYGEDATPGTGYTGLAAIQLPKKAGDITNKATKEKESDEEEEEEEEGNENEESEAEVDENEQGINGTSTNSTEAENGNGSSGGDNGEEGEEESVTGANAEDTTETGRQGKGTSKTTTSPNGGFEPTTPPQVYRTTSPPFGKTTTVEYEGEYEYTGANEYDNGYEIYESENGEPRGDNYRAYEDEYSYFKGQGYDGYDGQNYYHHQ

Target ORF information:

RefSeq Version	NM_004967.4
Organism	Homo sapiens(human)
Definition	Homo sapiens integrin binding sialoprotein (IBSP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004967.4

ORF Insert Sequence:

ATGAAGACTG CTTTAATTTT GCTCAGCATT TTGGGAATGG CCTGTGCTTT CTCAATGAAA 
AATTTGCATC GAAGAGTCAA AATAGAGGAT TCTGAAGAAA ATGGGGTCTT TAAGTACAGG 
CCACGATATT ATCTTTACAA GCATGCCTAC TTTTATCCTC ATTTAAAACG ATTTCCAGTT 
CAGGGCAGTA GTGACTCATC CGAAGAAAAT GGAGATGACA GTTCAGAAGA GGAGGAGGAA 
GAAGAGGAGA CTTCAAATGA AGGAGAAAAC AATGAAGAAT CGAATGAAGA TGAAGACTCT 
GAGGCTGAGA ATACCACACT TTCTGCTACA ACACTGGGCT ATGGAGAGGA CGCCACGCCT 
GGCACAGGGT ATACAGGGTT AGCTGCAATC CAGCTTCCCA AGAAGGCTGG GGATATAACA 
AATAAAGCTA CAAAAGAGAA GGAAAGTGAT GAAGAAGAAG AGGAGGAAGA GGAAGGAAAT 
GAAAACGAAG AAAGCGAAGC AGAAGTGGAT GAAAACGAAC AAGGCATAAA CGGCACCAGT 
ACCAACAGCA CAGAGGCAGA AAACGGCAAC GGCAGCAGCG GAGGAGACAA TGGAGAAGAA 
GGGGAAGAAG AAAGTGTCAC TGGAGCCAAT GCAGAAGACA CCACAGAGAC CGGAAGGCAG 
GGCAAGGGCA CCTCGAAGAC AACAACCTCT CCAAATGGTG GGTTTGAACC TACAACCCCA 
CCACAAGTCT ATAGAACCAC TTCCCCACCT TTTGGGAAAA CCACCACCGT TGAATACGAG 
GGGGAGTACG AATACACGGG CGCCAATGAA TACGACAATG GATATGAAAT CTATGAAAGT 
GAGAACGGGG AACCTCGTGG GGACAATTAC CGAGCCTATG AAGATGAGTA CAGCTACTTT 
AAAGGACAAG GCTACGATGG CTATGATGGT CAGAATTACT ACCACCACCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IBSP ( NM_004967.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004958.2 Homo sapiens integrin binding sialoprotein (IBSP), mRNA.

Price & Availability↑

CloneID	OHu08955
Clone ID Related Accession (Same CDS sequence)	NM_004967.4 , NM_004967.3
Accession Version	NM_004967.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	bone sialoprotein 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC093768.3, AI751848.1, J05213.1, DV462680.1, BQ573754.1 and AA452255.1. On Feb 8, 2008 this sequence version replaced NM_004967.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.193979.1, SRR1660807.136311.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2153307 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAAGACTG CTTTAATTTT GCTCAGCATT TTGGGAATGG CCTGTGCTTT CTCAATGAAA 
AATTTGCATC GAAGAGTCAA AATAGAGGAT TCTGAAGAAA ATGGGGTCTT TAAGTACAGG 
CCACGATATT ATCTTTACAA GCATGCCTAC TTTTATCCTC ATTTAAAACG ATTTCCAGTT 
CAGGGCAGTA GTGACTCATC CGAAGAAAAT GGAGATGACA GTTCAGAAGA GGAGGAGGAA 
GAAGAGGAGA CTTCAAATGA AGGAGAAAAC AATGAAGAAT CGAATGAAGA TGAAGACTCT 
GAGGCTGAGA ATACCACACT TTCTGCTACA ACACTGGGCT ATGGAGAGGA CGCCACGCCT 
GGCACAGGGT ATACAGGGTT AGCTGCAATC CAGCTTCCCA AGAAGGCTGG GGATATAACA 
AATAAAGCTA CAAAAGAGAA GGAAAGTGAT GAAGAAGAAG AGGAGGAAGA GGAAGGAAAT 
GAAAACGAAG AAAGCGAAGC AGAAGTGGAT GAAAACGAAC AAGGCATAAA CGGCACCAGT 
ACCAACAGCA CAGAGGCAGA AAACGGCAAC GGCAGCAGCG GAGGAGACAA TGGAGAAGAA 
GGGGAAGAAG AAAGTGTCAC TGGAGCCAAT GCAGAAGACA CCACAGAGAC CGGAAGGCAG 
GGCAAGGGCA CCTCGAAGAC AACAACCTCT CCAAATGGTG GGTTTGAACC TACAACCCCA 
CCACAAGTCT ATAGAACCAC TTCCCCACCT TTTGGGAAAA CCACCACCGT TGAATACGAG 
GGGGAGTACG AATACACGGG CGCCAATGAA TACGACAATG GATATGAAAT CTATGAAAGT 
GAGAACGGGG AACCTCGTGG GGACAATTAC CGAGCCTATG AAGATGAGTA CAGCTACTTT 
AAAGGACAAG GCTACGATGG CTATGATGGT CAGAATTACT ACCACCACCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004958.2
CDS	99..1052
Translation	MKTALILLSILGMACAFSMKNLHRRVKIEDSEENGVFKYRPRYYLYKHAYFYPHLKRFPVQGSSDSSEENGDDSSEEEEEEEETSNEGENNEESNEDEDSEAENTTLSATTLGYGEDATPGTGYTGLAAIQLPKKAGDITNKATKEKESDEEEEEEEEGNENEESEAEVDENEQGINGTSTNSTEAENGNGSSGGDNGEEGEEESVTGANAEDTTETGRQGKGTSKTTTSPNGGFEPTTPPQVYRTTSPPFGKTTTVEYEGEYEYTGANEYDNGYEIYESENGEPRGDNYRAYEDEYSYFKGQGYDGYDGQNYYHHQ

Target ORF information:

RefSeq Version	NM_004967.3
Organism	Homo sapiens(human)
Definition	Homo sapiens integrin binding sialoprotein (IBSP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004967.3

ORF Insert Sequence:

ATGAAGACTG CTTTAATTTT GCTCAGCATT TTGGGAATGG CCTGTGCTTT CTCAATGAAA 
AATTTGCATC GAAGAGTCAA AATAGAGGAT TCTGAAGAAA ATGGGGTCTT TAAGTACAGG 
CCACGATATT ATCTTTACAA GCATGCCTAC TTTTATCCTC ATTTAAAACG ATTTCCAGTT 
CAGGGCAGTA GTGACTCATC CGAAGAAAAT GGAGATGACA GTTCAGAAGA GGAGGAGGAA 
GAAGAGGAGA CTTCAAATGA AGGAGAAAAC AATGAAGAAT CGAATGAAGA TGAAGACTCT 
GAGGCTGAGA ATACCACACT TTCTGCTACA ACACTGGGCT ATGGAGAGGA CGCCACGCCT 
GGCACAGGGT ATACAGGGTT AGCTGCAATC CAGCTTCCCA AGAAGGCTGG GGATATAACA 
AATAAAGCTA CAAAAGAGAA GGAAAGTGAT GAAGAAGAAG AGGAGGAAGA GGAAGGAAAT 
GAAAACGAAG AAAGCGAAGC AGAAGTGGAT GAAAACGAAC AAGGCATAAA CGGCACCAGT 
ACCAACAGCA CAGAGGCAGA AAACGGCAAC GGCAGCAGCG GAGGAGACAA TGGAGAAGAA 
GGGGAAGAAG AAAGTGTCAC TGGAGCCAAT GCAGAAGACA CCACAGAGAC CGGAAGGCAG 
GGCAAGGGCA CCTCGAAGAC AACAACCTCT CCAAATGGTG GGTTTGAACC TACAACCCCA 
CCACAAGTCT ATAGAACCAC TTCCCCACCT TTTGGGAAAA CCACCACCGT TGAATACGAG 
GGGGAGTACG AATACACGGG CGCCAATGAA TACGACAATG GATATGAAAT CTATGAAAGT 
GAGAACGGGG AACCTCGTGG GGACAATTAC CGAGCCTATG AAGATGAGTA CAGCTACTTT 
AAAGGACAAG GCTACGATGG CTATGATGGT CAGAATTACT ACCACCACCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cancer Secretome May Influence BSP and DSP Expression in Human Salivary Gland Cells.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society65(3)139-151(2017 03)
Hamilton SL,Ferando B,Eapen AS,Yu JC,Joy AR

Serum levels of PSA, ALP, ICTP, and BSP in prostate cancer patients and the significance of ROC curve in the diagnosis of prostate cancer bone metastases.
Genetics and molecular research : GMR15(2)(2016 Jun)
Wei RJ,Li TY,Yang XC,Jia N,Yang XL,Song HB

Preameloblast-Derived Factors Mediate Osteoblast Differentiation of Human Bone Marrow Mesenchymal Stem Cells by Runx2-Osterix-BSP Signaling.
Tissue engineering. Part A22(1-2)93-102(2016 Jan)
Choung HW,Lee DS,Lee HK,Shon WJ,Park JC

Endochondral ossification pathway genes and postmenopausal osteoporosis: Association and specific allele related serum bone sialoprotein levels in Han Chinese.
Scientific reports516783(2015 Nov)
Zhang Y,Liu H,Zhang C,Zhang T,Zhang B,Li L,Chen G,Fu D,Wang K

Correlation between calcification and bone sialoprotein and osteopontin in papillary thyroid carcinoma.
International journal of clinical and experimental pathology8(2)2010-7(2015)
Wu G,Guo JJ,Ma ZY,Wang J,Zhou ZW,Wang Y

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IBSP cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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