RPA4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPA4
Entrez Gene ID	29935
Full Name	replication protein A4
Synonyms	HSU24186
General protein information	Preferred Names replication protein A4 Names replication protein A 30 kDa subunit RF-A protein 4 RP-A p30 replication factor A protein 4 replication protein A complex 34 kd subunit homolog Rpa4 replication protein A4, 30kDa replication protein A4, 34kDa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq21.33
Summary	This gene encodes a single-stranded DNA-binding protein that is the 30-kDa subunit of the replication protein A complex. Replication protein A is an essential factor for DNA double-strand break repair and cell cycle checkpoint activation. The encoded protein localizes to DNA repair foci and may be involved in the cellular DNA damage response. This protein may also play a role in inhibiting viral replication.[provided by RefSeq, Apr 2010].
Disorder MIM:	300767

mRNA and Protein(s)

mRNA	Protein	Name
NM_013347.4	NP_037479.1	replication protein A 30 kDa subunit

Pathways from BioSystems

Homologies

	RPA4	XP_001088106.1
Pan troglodytes (chimpanzee)	RPA4	XP_001136507.1
Homo sapiens (human)	RPA4	NP_037479.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic susceptibility to distinct bladder cancer subphenotypes.
Guey LT, Garc?a-Closas M, Murta-Nascimento C, Lloreta J, Palencia L, Kogevinas M, Rothman N, Vellalta G, Calle ML, Marenne G, Tard?n A, Carrato A, Garc?a-Closas R, Serra C, Silverman DT, Chanock S, Real FX, Malats N,
European urology57(2)283-92(2010 Feb)

An alternative form of replication protein a expressed in normal human tissues supports DNA repair.
Kemp MG, Mason AC, Carreira A, Reardon JT, Haring SJ, Borgstahl GE, Kowalczykowski SC, Sancar A, Wold MS
The Journal of biological chemistry285(7)4788-97(2010 Feb)

A naturally occurring human RPA subunit homolog does not support DNA replication or cell-cycle progression.
Haring SJ, Humphreys TD, Wold MS
Nucleic acids research38(3)846-58(2010 Jan)

Polymorphisms in innate immunity genes and lung cancer risk in Xuanwei, China.
Shen M, Vermeulen R, Rajaraman P, Menashe I, He X, Chapman RS, Yeager M, Thomas G, Burdett L, Hutchinson A, Yuenger J, Chanock S, Lan Q
Environmental and molecular mutagenesis50(4)285-90(2009 May)

An alternative form of replication protein a prevents viral replication in vitro.
Mason AC, Haring SJ, Pryor JM, Staloch CA, Gan TF, Wold MS
The Journal of biological chemistry284(8)5324-31(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic susceptibility to distinct bladder cancer subphenotypes.

Findings provide the first direct evidence for the function of alternative form of RPA in human DNA metabolism and support a model for aRPA functioning in chromosome maintenance functions in nonproliferating cells.
Title: An alternative form of replication protein a expressed in normal human tissues supports DNA repair.

Results suggest that RPA4 cannot support cell proliferation but can support processes that maintain the genomic integrity of the cell.
Title: A naturally occurring human RPA subunit homolog does not support DNA replication or cell-cycle progression.

Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Polymorphisms in innate immunity genes and lung cancer risk in Xuanwei, China.

Observational study of gene-disease association. (HuGE Navigator)
Title: Pathway-based evaluation of 380 candidate genes and lung cancer susceptibility suggests the importance of the cell cycle pathway.

The following RPA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu21547	NM_013347.4 Latest version!	Homo sapiens replication protein A4 (RPA4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPA4 ( NM_013347.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_037479.1 Homo sapiens replication protein A4 (RPA4), mRNA.

Price & Availability↑

CloneID	OHu21547
Clone ID Related Accession (Same CDS sequence)	NM_013347.4
Accession Version	NM_013347.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-29
Organism	Homo sapiens(human)
Product	replication protein A 30 kDa subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Z86061.1, BC069791.1 and U24186.2. This sequence is a reference standard in the RefSeqGene project. On Apr 25, 2010 this sequence version replaced NM_013347.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript is intronless :: U24186.2 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000373040.4/ ENSP00000362131.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGAGTAAGA GTGGGTTTGG GAGCTATGGC AGCATTTCTG CTGCTGATGG AGCGAGTGGA 
GGCAGTGACC AACTGTGTGA GAGAGATGCA ACTCCTGCTA TTAAGACCCA AAGACCTAAG 
GTCCGAATTC AGGACGTTGT ACCGTGTAAT GTGAACCAGC TTCTCAGCTC TACTGTGTTT 
GACCCTGTGT TCAAGGTTAG GGGAATTATA GTTTCCCAGG TCTCCATCGT GGGGGTAATC 
AGAGGGGCAG AGAAGGCTTC AAATCACATT TGTTACAAAA TTGATGATAT GACCGCGAAA 
CCAATCGAGG CCCGACAGTG GTTTGGTAGA GAGAAAGTCA AGCAGGTGAC TCCATTGTCA 
GTCGGAGTAT ATGTCAAAGT GTTTGGTATC CTCAAATGTC CCACGGGAAC AAAGAGCCTT 
GAGGTATTGA AAATTCATGT CCTAGAGGAC ATGAACGAGT TCACCGTGCA TATTCTGGAA 
ACGGTCAATG CACACATGAT GCTGGATAAA GCCCGTCGTG ATACCACTGT AGAAAGTGTG 
CCTGTGTCTC CATCAGAAGT GAATGATGCT GGGGATAACG ATGAGAGTCA CCGCAATTTC 
ATCCAGGACG AAGTGCTGCG TTTGATTCAT GAGTGTCCTC ATCAGGAAGG GAAGAGCATC 
CATGAGCTCC GGGCTCAGCT CTGCGACCTT AGCGTCAAGG CCATCAAGGA AGCGATTGAT 
TATCTGACCG TTGAGGGCCA CATCTATCCC ACTGTGGATC GGGAGCATTT TAAGTCTGCT 
GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037479.1
CDS	404..1189
Translation	MSKSGFGSYGSISAADGASGGSDQLCERDATPAIKTQRPKVRIQDVVPCNVNQLLSSTVFDPVFKVRGIIVSQVSIVGVIRGAEKASNHICYKIDDMTAKPIEARQWFGREKVKQVTPLSVGVYVKVFGILKCPTGTKSLEVLKIHVLEDMNEFTVHILETVNAHMMLDKARRDTTVESVPVSPSEVNDAGDNDESHRNFIQDEVLRLIHECPHQEGKSIHELRAQLCDLSVKAIKEAIDYLTVEGHIYPTVDREHFKSAD

Target ORF information:

RefSeq Version	NM_013347.4
Organism	Homo sapiens(human)
Definition	Homo sapiens replication protein A4 (RPA4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013347.4

ORF Insert Sequence:

ATGAGTAAGA GTGGGTTTGG GAGCTATGGC AGCATTTCTG CTGCTGATGG AGCGAGTGGA 
GGCAGTGACC AACTGTGTGA GAGAGATGCA ACTCCTGCTA TTAAGACCCA AAGACCTAAG 
GTCCGAATTC AGGACGTTGT ACCGTGTAAT GTGAACCAGC TTCTCAGCTC TACTGTGTTT 
GACCCTGTGT TCAAGGTTAG GGGAATTATA GTTTCCCAGG TCTCCATCGT GGGGGTAATC 
AGAGGGGCAG AGAAGGCTTC AAATCACATT TGTTACAAAA TTGATGATAT GACCGCGAAA 
CCAATCGAGG CCCGACAGTG GTTTGGTAGA GAGAAAGTCA AGCAGGTGAC TCCATTGTCA 
GTCGGAGTAT ATGTCAAAGT GTTTGGTATC CTCAAATGTC CCACGGGAAC AAAGAGCCTT 
GAGGTATTGA AAATTCATGT CCTAGAGGAC ATGAACGAGT TCACCGTGCA TATTCTGGAA 
ACGGTCAATG CACACATGAT GCTGGATAAA GCCCGTCGTG ATACCACTGT AGAAAGTGTG 
CCTGTGTCTC CATCAGAAGT GAATGATGCT GGGGATAACG ATGAGAGTCA CCGCAATTTC 
ATCCAGGACG AAGTGCTGCG TTTGATTCAT GAGTGTCCTC ATCAGGAAGG GAAGAGCATC 
CATGAGCTCC GGGCTCAGCT CTGCGACCTT AGCGTCAAGG CCATCAAGGA AGCGATTGAT 
TATCTGACCG TTGAGGGCCA CATCTATCCC ACTGTGGATC GGGAGCATTT TAAGTCTGCT 
GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic susceptibility to distinct bladder cancer subphenotypes.
European urology57(2)283-92(2010 Feb)
Guey LT,Garc?a-Closas M,Murta-Nascimento C,Lloreta J,Palencia L,Kogevinas M,Rothman N,Vellalta G,Calle ML,Marenne G,Tard?n A,Carrato A,Garc?a-Closas R,Serra C,Silverman DT,Chanock S,Real FX,Malats N,

An alternative form of replication protein a expressed in normal human tissues supports DNA repair.
The Journal of biological chemistry285(7)4788-97(2010 Feb)
Kemp MG,Mason AC,Carreira A,Reardon JT,Haring SJ,Borgstahl GE,Kowalczykowski SC,Sancar A,Wold MS

A naturally occurring human RPA subunit homolog does not support DNA replication or cell-cycle progression.
Nucleic acids research38(3)846-58(2010 Jan)
Haring SJ,Humphreys TD,Wold MS

Polymorphisms in innate immunity genes and lung cancer risk in Xuanwei, China.
Environmental and molecular mutagenesis50(4)285-90(2009 May)
Shen M,Vermeulen R,Rajaraman P,Menashe I,He X,Chapman RS,Yeager M,Thomas G,Burdett L,Hutchinson A,Yuenger J,Chanock S,Lan Q

An alternative form of replication protein a prevents viral replication in vitro.
The Journal of biological chemistry284(8)5324-31(2009 Feb)
Mason AC,Haring SJ,Pryor JM,Staloch CA,Gan TF,Wold MS

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

RPA4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報