CCDC22 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CCDC22
Entrez Gene ID	28952
Full Name	coiled-coil domain containing 22
Synonyms	CXorf37,JM1,RTSC2
General protein information	Preferred Names coiled-coil domain containing 22 Names coiled-coil domain-containing protein 22
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp11.23
Summary	This gene encodes a protein containing a coiled-coil domain. The encoded protein functions in the regulation of NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells) by interacting with COMMD (copper metabolism Murr1 domain-containing) proteins. The mouse orthologous protein has been shown to bind copines, which are calcium-dependent, membrane-binding proteins that may function in calcium signaling. This human gene has been identified as a novel candidate gene for syndromic X-linked intellectual disability. [provided by RefSeq, Aug 2013].
Disorder MIM:	300859
Disorder Html:	Ritscher-Schinzel syndrome 2, 300963 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005272599.2	XP_005272656.1	coiled-coil domain-containing protein 22 isoform X1
NM_014008.4	NP_054727.1	coiled-coil domain-containing protein 22
NM_014008.5	NP_054727.1	coiled-coil domain-containing protein 22
XM_005272599.3	XP_005272656.1	coiled-coil domain-containing protein 22 isoform X1
XM_005272599.4	XP_005272656.1	coiled-coil domain-containing protein 22 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CCDC22	NP_001069484.1
Xenopus tropicalis (tropical clawed frog)	ccdc22	NP_001004994.1
Homo sapiens (human)	CCDC22	NP_054727.1
Arabidopsis thaliana (thale cress)	AT1G55830	NP_001185244.1
Pan troglodytes (chimpanzee)	CCDC22	XP_001143086.2
	CCDC22	XP_001106217.1
Mus musculus (house mouse)	Ccdc22	NP_613069.3
Danio rerio (zebrafish)	ccdc22	NP_001020656.1
Canis lupus familiaris (dog)	CCDC22	XP_548995.2
Rattus norvegicus (Norway rat)	Ccdc22	NP_001129309.1

Gene Ontology
Bibliography

Related articles in PubMed

Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling.
McNally KE, Faulkner R, Steinberg F, Gallon M, Ghai R, Pim D, Langton P, Pearson N, Danson CM, N?gele H, Morris LL, Singla A, Overlee BL, Heesom KJ, Sessions R, Banks L, Collins BM, Berger I, Billadeau DD, Burstein E, Cullen PJ
Nature cell biology19(10)1214-1225(2017 Oct)

CCDC22 gene polymorphism is associated with advanced stages of endometriosis in a sample of Brazilian women.
de Oliveira Francisco D, de Paula Andres M, Gueuvoghlanian-Silva BY, Podgaec S, Fridman C
Journal of assisted reproduction and genetics34(7)939-944(2017 Jul)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Association between rs2294020 in X-linked CCDC22 and susceptibility to autoimmune diseases with focus on systemic lupus erythematosus.
D'Amico F, Skarmoutsou E, Lo LJ, Granata M, Trovato C, Rossi GA, Bellocchi C, Marchini M, Scorza R, Mazzarino MC, Keinan A
Immunology letters18158-62(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results suggest that rs2294020 is associated with the risk of several autoimmune diseases in European populations, specifically with diseases that present themselves, among else, in the skin.
Title: Association between rs2294020 in X-linked CCDC22 and susceptibility to autoimmune diseases with focus on systemic lupus erythematosus.

CCDC22 mutation is associated with hypercholesterolemia.
Title: CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL.

Missense variant in CCDC22 causes X-linked recessive intellectual disability with features of Ritscher-Schinzel/3C syndrome
Title: Missense variant in CCDC22 causes X-linked recessive intellectual disability with features of Ritscher-Schinzel/3C syndrome.

CCDC22 participates in NF-kappaB activation and its deficiency leads to decreased IkappaB turnover
Title: CCDC22 deficiency in humans blunts activation of proinflammatory NF-?B signaling.

This study demonistrated that CCDC22 is a novel candidate gene for syndromic X-linked intellectual disability.
Title: CCDC22: a novel candidate gene for syndromic X-linked intellectual disability.

The following CCDC22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCDC22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20452	NM_014008.5 Latest version!	Homo sapiens coiled-coil domain containing 22 (CCDC22), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu53992	XM_005272599.4 Latest version!	Homo sapiens coiled-coil domain containing 22 (CCDC22), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
View Old Accession Versions >>
OHu20452	NM_014008.4	Homo sapiens coiled-coil domain containing 22 (CCDC22), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu53992	XM_005272599.2	Homo sapiens coiled-coil domain containing 22 (CCDC22), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu53992	XM_005272599.3	Homo sapiens coiled-coil domain containing 22 (CCDC22), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CCDC22 ( NM_014008.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_054727.1 Homo sapiens coiled-coil domain containing 22 (CCDC22), mRNA.

Price & Availability↑

CloneID	OHu20452
Clone ID Related Accession (Same CDS sequence)	NM_014008.4 , NM_014008.5
Accession Version	NM_014008.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	coiled-coil domain-containing protein 22
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM146102.1, DC340647.1, AJ005890.1 and BC000972.2. This sequence is a reference standard in the RefSeqGene project. On Aug 22, 2013 this sequence version replaced NM_014008.3. ##Evidence-Data-START## Transcript exon combination :: BC000972.2, SRR1803614.180387.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGG CGGACCGAAT CCTCATCCAT TCGCTGCGCC AGGCCGGCAC GGCAGTTCCT 
CCAGATGTGC AGACCTTGCG CGCCTTCACC ACTGAGCTGG TTGTAGAGGC TGTGGTCCGC 
TGCCTGCGTG TGATCAACCC TGCGGTGGGC TCTGGCCTCA GCCCTCTGCT GCCTCTTGCC 
ATGTCTGCCC GGTTCCGCCT GGCCATGAGC CTGGCTCAGG CCTGCATGGA CCTGGGCTAT 
CCCTTGGAGC TTGGCTATCA GAACTTCCTC TACCCCAGTG AGCCTGACCT CCGAGACCTG 
CTTCTCTTCT TGGCTGAGCG TCTGCCCACC GATGCCTCTG AGGATGCAGA CCAGCCTGCA 
GGTGACTCAG CTATTCTCCT CCGGGCCATT GGGAGCCAAA TTCGGGACCA GCTGGCACTG 
CCTTGGGTCC CGCCCCACCT TCGCACTCCC AAGCTGCAGC ACCTCCAGGG CTCGGCCCTC 
CAGAAGCCTT TCCATGCCAG CAGGCTGGTC GTGCCAGAAT TGAGTTCCAG AGGTGAGCCA 
CGGGAGTTCC AGGCGAGTCC CCTGCTGCTT CCAGTCCCTA CCCAGGTGCC TCAGCCTGTT 
GGAAGGGTGG CCTCGCTCCT CGAACACCAT GCCCTGCAGC TCTGCCAGCA GACGGGCCGG 
GACCGGCCAG GGGATGAGGA CTGGGTCCAC CGGACATCCC GCCTCCCACC CCAGGAGGAC 
ACACGGGCTC AGCGGCAGCG GCTGCAAAAG CAACTGACTG AGCATCTGCG CCAAAGCTGG 
GGCCTGCTTG GGGCCCCCAT ACAAGCCCGG GACCTGGGAG AACTGCTGCA GGCCTGGGGT 
GCTGGGGCCA AGACTGGTGC TCCTAAGGGC TCCCGCTTCA CGCACTCAGA GAAGTTCACC 
TTCCATCTGG AGCCCCAGGC CCAGGCCACT CAGGTGTCAG ATGTGCCAGC CACCTCCCGG 
CGGCCTGAAC AGGTCACGTG GGCAGCTCAG GAACAGGAGC TCGAGTCCCT TCGGGAGCAG 
CTGGAAGGAG TGAACCGCAG CATTGAGGAG GTTGAGGCCG ACATGAAGAC CCTGGGCGTC 
AGCTTTGTGC AGGCAGAGTC TGAGTGCCGG CACAGCAAGC TCAGTACAGC AGAGCGTGAG 
CAGGCCCTGC GCCTGAAGAG CCGCGCGGTG GAGCTGCTGC CCGATGGGAC TGCCAACCTT 
GCCAAGCTGC AGCTTGTGGT GGAGAATAGT GCCCAGCGGG TCATCCACTT GGCGGGTCAG 
TGGGAGAAGC ACCGGGTCCC ACTCCTCGCT GAGTACCGCC ACCTCCGAAA GCTGCAGGAT 
TGCAGAGAGC TGGAATCTTC TCGACGGCTG GCAGAGATCC AAGAACTGCA CCAGAGTGTC 
CGGGCGGCTG CTGAAGAGGC CCGCAGGAAG GAGGAGGTCT ATAAGCAGCT GATGTCAGAG 
CTGGAGACTC TGCCCAGAGA TGTGTCCCGG CTGGCCTACA CCCAGCGCAT CCTGGAGATC 
GTGGGCAACA TCCGGAAGCA GAAGGAAGAG ATCACCAAGA TCTTGTCTGA TACGAAGGAG 
CTTCAGAAGG AAATCAACTC CCTATCTGGG AAGCTGGACC GGACGTTTGC GGTGACTGAT 
GAGCTTGTGT TCAAGGATGC CAAGAAGGAC GATGCTGTTC GGAAGGCCTA TAAGTATCTA 
GCTGCTCTGC ACGAGAACTG CAGCCAGCTC ATCCAGACCA TCGAGGACAC AGGCACCATC 
ATGCGGGAGG TTCGAGACCT CGAGGAGCAG ATCGAGACAG AGCTGGGCAA GAAGACCCTC 
AGCAACCTGG AGAAGATCCG GGAGGACTAC CGAGCCCTCC GCCAGGAGAA CGCTGGCCTC 
CTAGGCCGGG TCCGGGAGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054727.1
CDS	171..2054
Translation	MEEADRILIHSLRQAGTAVPPDVQTLRAFTTELVVEAVVRCLRVINPAVGSGLSPLLPLAMSARFRLAMSLAQACMDLGYPLELGYQNFLYPSEPDLRDLLLFLAERLPTDASEDADQPAGDSAILLRAIGSQIRDQLALPWVPPHLRTPKLQHLQGSALQKPFHASRLVVPELSSRGEPREFQASPLLLPVPTQVPQPVGRVASLLEHHALQLCQQTGRDRPGDEDWVHRTSRLPPQEDTRAQRQRLQKQLTEHLRQSWGLLGAPIQARDLGELLQAWGAGAKTGAPKGSRFTHSEKFTFHLEPQAQATQVSDVPATSRRPEQVTWAAQEQELESLREQLEGVNRSIEEVEADMKTLGVSFVQAESECRHSKLSTAEREQALRLKSRAVELLPDGTANLAKLQLVVENSAQRVIHLAGQWEKHRVPLLAEYRHLRKLQDCRELESSRRLAEIQELHQSVRAAAEEARRKEEVYKQLMSELETLPRDVSRLAYTQRILEIVGNIRKQKEEITKILSDTKELQKEINSLSGKLDRTFAVTDELVFKDAKKDDAVRKAYKYLAALHENCSQLIQTIEDTGTIMREVRDLEEQIETELGKKTLSNLEKIREDYRALRQENAGLLGRVREA

Target ORF information:

RefSeq Version	NM_014008.4
Organism	Homo sapiens(human)
Definition	Homo sapiens coiled-coil domain containing 22 (CCDC22), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014008.4

ORF Insert Sequence:

ATGGAGGAGG CGGACCGAAT CCTCATCCAT TCGCTGCGCC AGGCCGGCAC GGCAGTTCCT 
CCAGATGTGC AGACCTTGCG CGCCTTCACC ACTGAGCTGG TTGTAGAGGC TGTGGTCCGC 
TGCCTGCGTG TGATCAACCC TGCGGTGGGC TCTGGCCTCA GCCCTCTGCT GCCTCTTGCC 
ATGTCTGCCC GGTTCCGCCT GGCCATGAGC CTGGCTCAGG CCTGCATGGA CCTGGGCTAT 
CCCTTGGAGC TTGGCTATCA GAACTTCCTC TACCCCAGTG AGCCTGACCT CCGAGACCTG 
CTTCTCTTCT TGGCTGAGCG TCTGCCCACC GATGCCTCTG AGGATGCAGA CCAGCCTGCA 
GGTGACTCAG CTATTCTCCT CCGGGCCATT GGGAGCCAAA TTCGGGACCA GCTGGCACTG 
CCTTGGGTCC CGCCCCACCT TCGCACTCCC AAGCTGCAGC ACCTCCAGGG CTCGGCCCTC 
CAGAAGCCTT TCCATGCCAG CAGGCTGGTC GTGCCAGAAT TGAGTTCCAG AGGTGAGCCA 
CGGGAGTTCC AGGCGAGTCC CCTGCTGCTT CCAGTCCCTA CCCAGGTGCC TCAGCCTGTT 
GGAAGGGTGG CCTCGCTCCT CGAACACCAT GCCCTGCAGC TCTGCCAGCA GACGGGCCGG 
GACCGGCCAG GGGATGAGGA CTGGGTCCAC CGGACATCCC GCCTCCCACC CCAGGAGGAC 
ACACGGGCTC AGCGGCAGCG GCTGCAAAAG CAACTGACTG AGCATCTGCG CCAAAGCTGG 
GGCCTGCTTG GGGCCCCCAT ACAAGCCCGG GACCTGGGAG AACTGCTGCA GGCCTGGGGT 
GCTGGGGCCA AGACTGGTGC TCCTAAGGGC TCCCGCTTCA CGCACTCAGA GAAGTTCACC 
TTCCATCTGG AGCCCCAGGC CCAGGCCACT CAGGTGTCAG ATGTGCCAGC CACCTCCCGG 
CGGCCTGAAC AGGTCACGTG GGCAGCTCAG GAACAGGAGC TCGAGTCCCT TCGGGAGCAG 
CTGGAAGGAG TGAACCGCAG CATTGAGGAG GTTGAGGCCG ACATGAAGAC CCTGGGCGTC 
AGCTTTGTGC AGGCAGAGTC TGAGTGCCGG CACAGCAAGC TCAGTACAGC AGAGCGTGAG 
CAGGCCCTGC GCCTGAAGAG CCGCGCGGTG GAGCTGCTGC CCGATGGGAC TGCCAACCTT 
GCCAAGCTGC AGCTTGTGGT GGAGAATAGT GCCCAGCGGG TCATCCACTT GGCGGGTCAG 
TGGGAGAAGC ACCGGGTCCC ACTCCTCGCT GAGTACCGCC ACCTCCGAAA GCTGCAGGAT 
TGCAGAGAGC TGGAATCTTC TCGACGGCTG GCAGAGATCC AAGAACTGCA CCAGAGTGTC 
CGGGCGGCTG CTGAAGAGGC CCGCAGGAAG GAGGAGGTCT ATAAGCAGCT GATGTCAGAG 
CTGGAGACTC TGCCCAGAGA TGTGTCCCGG CTGGCCTACA CCCAGCGCAT CCTGGAGATC 
GTGGGCAACA TCCGGAAGCA GAAGGAAGAG ATCACCAAGA TCTTGTCTGA TACGAAGGAG 
CTTCAGAAGG AAATCAACTC CCTATCTGGG AAGCTGGACC GGACGTTTGC GGTGACTGAT 
GAGCTTGTGT TCAAGGATGC CAAGAAGGAC GATGCTGTTC GGAAGGCCTA TAAGTATCTA 
GCTGCTCTGC ACGAGAACTG CAGCCAGCTC ATCCAGACCA TCGAGGACAC AGGCACCATC 
ATGCGGGAGG TTCGAGACCT CGAGGAGCAG ATCGAGACAG AGCTGGGCAA GAAGACCCTC 
AGCAACCTGG AGAAGATCCG GGAGGACTAC CGAGCCCTCC GCCAGGAGAA CGCTGGCCTC 
CTAGGCCGGG TCCGGGAGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CCDC22 ( NM_014008.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_054727.1 Homo sapiens coiled-coil domain containing 22 (CCDC22), mRNA.

Price & Availability↑

CloneID	OHu20452
Clone ID Related Accession (Same CDS sequence)	NM_014008.4 , NM_014008.5
Accession Version	NM_014008.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	coiled-coil domain-containing protein 22
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC340647.1, AJ005890.1 and BC000972.2. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_014008.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.584118.1, SRR1803614.180387.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000376227.4/ ENSP00000365401.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGAGG CGGACCGAAT CCTCATCCAT TCGCTGCGCC AGGCCGGCAC GGCAGTTCCT 
CCAGATGTGC AGACCTTGCG CGCCTTCACC ACTGAGCTGG TTGTAGAGGC TGTGGTCCGC 
TGCCTGCGTG TGATCAACCC TGCGGTGGGC TCTGGCCTCA GCCCTCTGCT GCCTCTTGCC 
ATGTCTGCCC GGTTCCGCCT GGCCATGAGC CTGGCTCAGG CCTGCATGGA CCTGGGCTAT 
CCCTTGGAGC TTGGCTATCA GAACTTCCTC TACCCCAGTG AGCCTGACCT CCGAGACCTG 
CTTCTCTTCT TGGCTGAGCG TCTGCCCACC GATGCCTCTG AGGATGCAGA CCAGCCTGCA 
GGTGACTCAG CTATTCTCCT CCGGGCCATT GGGAGCCAAA TTCGGGACCA GCTGGCACTG 
CCTTGGGTCC CGCCCCACCT TCGCACTCCC AAGCTGCAGC ACCTCCAGGG CTCGGCCCTC 
CAGAAGCCTT TCCATGCCAG CAGGCTGGTC GTGCCAGAAT TGAGTTCCAG AGGTGAGCCA 
CGGGAGTTCC AGGCGAGTCC CCTGCTGCTT CCAGTCCCTA CCCAGGTGCC TCAGCCTGTT 
GGAAGGGTGG CCTCGCTCCT CGAACACCAT GCCCTGCAGC TCTGCCAGCA GACGGGCCGG 
GACCGGCCAG GGGATGAGGA CTGGGTCCAC CGGACATCCC GCCTCCCACC CCAGGAGGAC 
ACACGGGCTC AGCGGCAGCG GCTGCAAAAG CAACTGACTG AGCATCTGCG CCAAAGCTGG 
GGCCTGCTTG GGGCCCCCAT ACAAGCCCGG GACCTGGGAG AACTGCTGCA GGCCTGGGGT 
GCTGGGGCCA AGACTGGTGC TCCTAAGGGC TCCCGCTTCA CGCACTCAGA GAAGTTCACC 
TTCCATCTGG AGCCCCAGGC CCAGGCCACT CAGGTGTCAG ATGTGCCAGC CACCTCCCGG 
CGGCCTGAAC AGGTCACGTG GGCAGCTCAG GAACAGGAGC TCGAGTCCCT TCGGGAGCAG 
CTGGAAGGAG TGAACCGCAG CATTGAGGAG GTTGAGGCCG ACATGAAGAC CCTGGGCGTC 
AGCTTTGTGC AGGCAGAGTC TGAGTGCCGG CACAGCAAGC TCAGTACAGC AGAGCGTGAG 
CAGGCCCTGC GCCTGAAGAG CCGCGCGGTG GAGCTGCTGC CCGATGGGAC TGCCAACCTT 
GCCAAGCTGC AGCTTGTGGT GGAGAATAGT GCCCAGCGGG TCATCCACTT GGCGGGTCAG 
TGGGAGAAGC ACCGGGTCCC ACTCCTCGCT GAGTACCGCC ACCTCCGAAA GCTGCAGGAT 
TGCAGAGAGC TGGAATCTTC TCGACGGCTG GCAGAGATCC AAGAACTGCA CCAGAGTGTC 
CGGGCGGCTG CTGAAGAGGC CCGCAGGAAG GAGGAGGTCT ATAAGCAGCT GATGTCAGAG 
CTGGAGACTC TGCCCAGAGA TGTGTCCCGG CTGGCCTACA CCCAGCGCAT CCTGGAGATC 
GTGGGCAACA TCCGGAAGCA GAAGGAAGAG ATCACCAAGA TCTTGTCTGA TACGAAGGAG 
CTTCAGAAGG AAATCAACTC CCTATCTGGG AAGCTGGACC GGACGTTTGC GGTGACTGAT 
GAGCTTGTGT TCAAGGATGC CAAGAAGGAC GATGCTGTTC GGAAGGCCTA TAAGTATCTA 
GCTGCTCTGC ACGAGAACTG CAGCCAGCTC ATCCAGACCA TCGAGGACAC AGGCACCATC 
ATGCGGGAGG TTCGAGACCT CGAGGAGCAG ATCGAGACAG AGCTGGGCAA GAAGACCCTC 
AGCAACCTGG AGAAGATCCG GGAGGACTAC CGAGCCCTCC GCCAGGAGAA CGCTGGCCTC 
CTAGGCCGGG TCCGGGAGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054727.1
CDS	168..2051
Translation	MEEADRILIHSLRQAGTAVPPDVQTLRAFTTELVVEAVVRCLRVINPAVGSGLSPLLPLAMSARFRLAMSLAQACMDLGYPLELGYQNFLYPSEPDLRDLLLFLAERLPTDASEDADQPAGDSAILLRAIGSQIRDQLALPWVPPHLRTPKLQHLQGSALQKPFHASRLVVPELSSRGEPREFQASPLLLPVPTQVPQPVGRVASLLEHHALQLCQQTGRDRPGDEDWVHRTSRLPPQEDTRAQRQRLQKQLTEHLRQSWGLLGAPIQARDLGELLQAWGAGAKTGAPKGSRFTHSEKFTFHLEPQAQATQVSDVPATSRRPEQVTWAAQEQELESLREQLEGVNRSIEEVEADMKTLGVSFVQAESECRHSKLSTAEREQALRLKSRAVELLPDGTANLAKLQLVVENSAQRVIHLAGQWEKHRVPLLAEYRHLRKLQDCRELESSRRLAEIQELHQSVRAAAEEARRKEEVYKQLMSELETLPRDVSRLAYTQRILEIVGNIRKQKEEITKILSDTKELQKEINSLSGKLDRTFAVTDELVFKDAKKDDAVRKAYKYLAALHENCSQLIQTIEDTGTIMREVRDLEEQIETELGKKTLSNLEKIREDYRALRQENAGLLGRVREA

Target ORF information:

RefSeq Version	NM_014008.5
Organism	Homo sapiens(human)
Definition	Homo sapiens coiled-coil domain containing 22 (CCDC22), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014008.5

ORF Insert Sequence:

ATGGAGGAGG CGGACCGAAT CCTCATCCAT TCGCTGCGCC AGGCCGGCAC GGCAGTTCCT 
CCAGATGTGC AGACCTTGCG CGCCTTCACC ACTGAGCTGG TTGTAGAGGC TGTGGTCCGC 
TGCCTGCGTG TGATCAACCC TGCGGTGGGC TCTGGCCTCA GCCCTCTGCT GCCTCTTGCC 
ATGTCTGCCC GGTTCCGCCT GGCCATGAGC CTGGCTCAGG CCTGCATGGA CCTGGGCTAT 
CCCTTGGAGC TTGGCTATCA GAACTTCCTC TACCCCAGTG AGCCTGACCT CCGAGACCTG 
CTTCTCTTCT TGGCTGAGCG TCTGCCCACC GATGCCTCTG AGGATGCAGA CCAGCCTGCA 
GGTGACTCAG CTATTCTCCT CCGGGCCATT GGGAGCCAAA TTCGGGACCA GCTGGCACTG 
CCTTGGGTCC CGCCCCACCT TCGCACTCCC AAGCTGCAGC ACCTCCAGGG CTCGGCCCTC 
CAGAAGCCTT TCCATGCCAG CAGGCTGGTC GTGCCAGAAT TGAGTTCCAG AGGTGAGCCA 
CGGGAGTTCC AGGCGAGTCC CCTGCTGCTT CCAGTCCCTA CCCAGGTGCC TCAGCCTGTT 
GGAAGGGTGG CCTCGCTCCT CGAACACCAT GCCCTGCAGC TCTGCCAGCA GACGGGCCGG 
GACCGGCCAG GGGATGAGGA CTGGGTCCAC CGGACATCCC GCCTCCCACC CCAGGAGGAC 
ACACGGGCTC AGCGGCAGCG GCTGCAAAAG CAACTGACTG AGCATCTGCG CCAAAGCTGG 
GGCCTGCTTG GGGCCCCCAT ACAAGCCCGG GACCTGGGAG AACTGCTGCA GGCCTGGGGT 
GCTGGGGCCA AGACTGGTGC TCCTAAGGGC TCCCGCTTCA CGCACTCAGA GAAGTTCACC 
TTCCATCTGG AGCCCCAGGC CCAGGCCACT CAGGTGTCAG ATGTGCCAGC CACCTCCCGG 
CGGCCTGAAC AGGTCACGTG GGCAGCTCAG GAACAGGAGC TCGAGTCCCT TCGGGAGCAG 
CTGGAAGGAG TGAACCGCAG CATTGAGGAG GTTGAGGCCG ACATGAAGAC CCTGGGCGTC 
AGCTTTGTGC AGGCAGAGTC TGAGTGCCGG CACAGCAAGC TCAGTACAGC AGAGCGTGAG 
CAGGCCCTGC GCCTGAAGAG CCGCGCGGTG GAGCTGCTGC CCGATGGGAC TGCCAACCTT 
GCCAAGCTGC AGCTTGTGGT GGAGAATAGT GCCCAGCGGG TCATCCACTT GGCGGGTCAG 
TGGGAGAAGC ACCGGGTCCC ACTCCTCGCT GAGTACCGCC ACCTCCGAAA GCTGCAGGAT 
TGCAGAGAGC TGGAATCTTC TCGACGGCTG GCAGAGATCC AAGAACTGCA CCAGAGTGTC 
CGGGCGGCTG CTGAAGAGGC CCGCAGGAAG GAGGAGGTCT ATAAGCAGCT GATGTCAGAG 
CTGGAGACTC TGCCCAGAGA TGTGTCCCGG CTGGCCTACA CCCAGCGCAT CCTGGAGATC 
GTGGGCAACA TCCGGAAGCA GAAGGAAGAG ATCACCAAGA TCTTGTCTGA TACGAAGGAG 
CTTCAGAAGG AAATCAACTC CCTATCTGGG AAGCTGGACC GGACGTTTGC GGTGACTGAT 
GAGCTTGTGT TCAAGGATGC CAAGAAGGAC GATGCTGTTC GGAAGGCCTA TAAGTATCTA 
GCTGCTCTGC ACGAGAACTG CAGCCAGCTC ATCCAGACCA TCGAGGACAC AGGCACCATC 
ATGCGGGAGG TTCGAGACCT CGAGGAGCAG ATCGAGACAG AGCTGGGCAA GAAGACCCTC 
AGCAACCTGG AGAAGATCCG GGAGGACTAC CGAGCCCTCC GCCAGGAGAA CGCTGGCCTC 
CTAGGCCGGG TCCGGGAGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CCDC22 ( XM_005272599.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005272656.1 Homo sapiens coiled-coil domain containing 22 (CCDC22), transcript variant X1, mRNA.

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CloneID	OHu53992
Clone ID Related Accession (Same CDS sequence)	XM_005272599.2 , XM_005272599.3 , XM_005272599.4
Accession Version	XM_005272599.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	coiled-coil domain-containing protein 22 isoform X1
Comment

ATGGAGGAGG CGGACCGAAT CCTCATCCAT TCGCTGCGCC AGGCCGGCAC GGCAGTTCCT 
CCAGATGTGC AGACCTTGCG CGCCTTCACC ACTGAGCTGG TTGTAGAGGC TGTGGTCCGC 
TGCCTGCGTG TGATCAACCC TGCGGTGGGC TCTGGCCTCA GCCCTCTGCT GCCTCTTGCC 
ATGTCTGCCC GGTTCCGCCT GGCCATGAGC CTGGCTCAGG CCTGCATGGA CCTGGGCTAT 
CCCTTGGAGC TTGGCTATCA GAACTTCCTC TACCCCAGTG AGCCTGACCT CCGAGACCTG 
CTTCTCTTCT TGGCTGAGCG TCTGCCCACC GATGCCTCTG AGGATGCAGA CCAGCCTGCA 
GGTGACTCAG CTATTCTCCT CCGGGCCATT GGGAGCCAAA TTCGGGACCA GCTGGCACTG 
CCTTGGGTCC CGCCCCACCT TCGCACTCCC AAGCTGCAGC ACCTCCAGGG CTCGGCCCTC 
CAGAAGCCTT TCCATGCCAG CAGGCTGGTC GTGCCAGAAT TGAGTTCCAG AGAGCCACGG 
GAGTTCCAGG CGAGTCCCCT GCTGCTTCCA GTCCCTACCC AGGTGCCTCA GCCTGTTGGA 
AGGGTGGCCT CGCTCCTCGA ACACCATGCC CTGCAGCTCT GCCAGCAGAC GGGCCGGGAC 
CGGCCAGGGG ATGAGGACTG GGTCCACCGG ACATCCCGCC TCCCACCCCA GGAGGACACA 
CGGGCTCAGC GGCAGCGGCT GCAAAAGCAA CTGACTGAGC ATCTGCGCCA AAGCTGGGGC 
CTGCTTGGGG CCCCCATACA AGCCCGGGAC CTGGGAGAAC TGCTGCAGGC CTGGGGTGCT 
GGGGCCAAGA CTGGTGCTCC TAAGGGCTCC CGCTTCACGC ACTCAGAGAA GTTCACCTTC 
CATCTGGAGC CCCAGGCCCA GGCCACTCAG GTGTCAGATG TGCCAGCCAC CTCCCGGCGG 
CCTGAACAGG TCACGTGGGC AGCTCAGGAA CAGGAGCTCG AGTCCCTTCG GGAGCAGCTG 
GAAGGAGTGA ACCGCAGCAT TGAGGAGGTT GAGGCCGACA TGAAGACCCT GGGCGTCAGC 
TTTGTGCAGG CAGAGTCTGA GTGCCGGCAC AGCAAGCTCA GTACAGCAGA GCGTGAGCAG 
GCCCTGCGCC TGAAGAGCCG CGCGGTGGAG CTGCTGCCCG ATGGGACTGC CAACCTTGCC 
AAGCTGCAGC TTGTGGTGGA GAATAGTGCC CAGCGGGTCA TCCACTTGGC GGGTCAGTGG 
GAGAAGCACC GGGTCCCACT CCTCGCTGAG TACCGCCACC TCCGAAAGCT GCAGGATTGC 
AGAGAGCTGG AATCTTCTCG ACGGCTGGCA GAGATCCAAG AACTGCACCA GAGTGTCCGG 
GCGGCTGCTG AAGAGGCCCG CAGGAAGGAG GAGGTCTATA AGCAGCTGAT GTCAGAGCTG 
GAGACTCTGC CCAGAGATGT GTCCCGGCTG GCCTACACCC AGCGCATCCT GGAGATCGTG 
GGCAACATCC GGAAGCAGAA GGAAGAGATC ACCAAGATCT TGTCTGATAC GAAGGAGCTT 
CAGAAGGAAA TCAACTCCCT ATCTGGGAAG CTGGACCGGA CGTTTGCGGT GACTGATGAG 
CTTGTGTTCA AGGATGCCAA GAAGGACGAT GCTGTTCGGA AGGCCTATAA GTATCTAGCT 
GCTCTGCACG AGAACTGCAG CCAGCTCATC CAGACCATCG AGGACACAGG CACCATCATG 
CGGGAGGTTC GAGACCTCGA GGAGCAGATC GAGACAGAGC TGGGCAAGAA GACCCTCAGC 
AACCTGGAGA AGATCCGGGA GGACTACCGA GCCCTCCGCC AGGAGAACGC TGGCCTCCTA 
GGCCGGGTCC GGGAGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_005272656.1

Translation

MEEADRILIHSLRQAGTAVPPDVQTLRAFTTELVVEAVVRCLRVINPAVGSGLSPLLPLAMSARFRLAMSLAQACMDLGYPLELGYQNFLYPSEPDLRDLLLFLAERLPTDASEDADQPAGDSAILLRAIGSQIRDQLALPWVPPHLRTPKLQHLQGSALQKPFHASRLVVPELSSREPREFQASPLLLPVPTQVPQPVGRVASLLEHHALQLCQQTGRDRPGDEDWVHRTSRLPPQEDTRAQRQRLQKQLTEHLRQSWGLLGAPIQARDLGELLQAWGAGAKTGAPKGSRFTHSEKFTFHLEPQAQATQVSDVPATSRRPEQVTWAAQEQELESLREQLEGVNRSIEEVEADMKTLGVSFVQAESECRHSKLSTAEREQALRLKSRAVELLPDGTANLAKLQLVVENSAQRVIHLAGQWEKHRVPLLAEYRHLRKLQDCRELESSRRLAEIQELHQSVRAAAEEARRKEEVYKQLMSELETLPRDVSRLAYTQRILEIVGNIRKQKEEITKILSDTKELQKEINSLSGKLDRTFAVTDELVFKDAKKDDAVRKAYKYLAALHENCSQLIQTIEDTGTIMREVRDLEEQIETELGKKTLSNLEKIREDYRALRQENAGLLGRVREA

Target ORF information:

RefSeq Version	XM_005272599.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens coiled-coil domain containing 22 (CCDC22), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005272599.2

ORF Insert Sequence:

ATGGAGGAGG CGGACCGAAT CCTCATCCAT TCGCTGCGCC AGGCCGGCAC GGCAGTTCCT 
CCAGATGTGC AGACCTTGCG CGCCTTCACC ACTGAGCTGG TTGTAGAGGC TGTGGTCCGC 
TGCCTGCGTG TGATCAACCC TGCGGTGGGC TCTGGCCTCA GCCCTCTGCT GCCTCTTGCC 
ATGTCTGCCC GGTTCCGCCT GGCCATGAGC CTGGCTCAGG CCTGCATGGA CCTGGGCTAT 
CCCTTGGAGC TTGGCTATCA GAACTTCCTC TACCCCAGTG AGCCTGACCT CCGAGACCTG 
CTTCTCTTCT TGGCTGAGCG TCTGCCCACC GATGCCTCTG AGGATGCAGA CCAGCCTGCA 
GGTGACTCAG CTATTCTCCT CCGGGCCATT GGGAGCCAAA TTCGGGACCA GCTGGCACTG 
CCTTGGGTCC CGCCCCACCT TCGCACTCCC AAGCTGCAGC ACCTCCAGGG CTCGGCCCTC 
CAGAAGCCTT TCCATGCCAG CAGGCTGGTC GTGCCAGAAT TGAGTTCCAG AGAGCCACGG 
GAGTTCCAGG CGAGTCCCCT GCTGCTTCCA GTCCCTACCC AGGTGCCTCA GCCTGTTGGA 
AGGGTGGCCT CGCTCCTCGA ACACCATGCC CTGCAGCTCT GCCAGCAGAC GGGCCGGGAC 
CGGCCAGGGG ATGAGGACTG GGTCCACCGG ACATCCCGCC TCCCACCCCA GGAGGACACA 
CGGGCTCAGC GGCAGCGGCT GCAAAAGCAA CTGACTGAGC ATCTGCGCCA AAGCTGGGGC 
CTGCTTGGGG CCCCCATACA AGCCCGGGAC CTGGGAGAAC TGCTGCAGGC CTGGGGTGCT 
GGGGCCAAGA CTGGTGCTCC TAAGGGCTCC CGCTTCACGC ACTCAGAGAA GTTCACCTTC 
CATCTGGAGC CCCAGGCCCA GGCCACTCAG GTGTCAGATG TGCCAGCCAC CTCCCGGCGG 
CCTGAACAGG TCACGTGGGC AGCTCAGGAA CAGGAGCTCG AGTCCCTTCG GGAGCAGCTG 
GAAGGAGTGA ACCGCAGCAT TGAGGAGGTT GAGGCCGACA TGAAGACCCT GGGCGTCAGC 
TTTGTGCAGG CAGAGTCTGA GTGCCGGCAC AGCAAGCTCA GTACAGCAGA GCGTGAGCAG 
GCCCTGCGCC TGAAGAGCCG CGCGGTGGAG CTGCTGCCCG ATGGGACTGC CAACCTTGCC 
AAGCTGCAGC TTGTGGTGGA GAATAGTGCC CAGCGGGTCA TCCACTTGGC GGGTCAGTGG 
GAGAAGCACC GGGTCCCACT CCTCGCTGAG TACCGCCACC TCCGAAAGCT GCAGGATTGC 
AGAGAGCTGG AATCTTCTCG ACGGCTGGCA GAGATCCAAG AACTGCACCA GAGTGTCCGG 
GCGGCTGCTG AAGAGGCCCG CAGGAAGGAG GAGGTCTATA AGCAGCTGAT GTCAGAGCTG 
GAGACTCTGC CCAGAGATGT GTCCCGGCTG GCCTACACCC AGCGCATCCT GGAGATCGTG 
GGCAACATCC GGAAGCAGAA GGAAGAGATC ACCAAGATCT TGTCTGATAC GAAGGAGCTT 
CAGAAGGAAA TCAACTCCCT ATCTGGGAAG CTGGACCGGA CGTTTGCGGT GACTGATGAG 
CTTGTGTTCA AGGATGCCAA GAAGGACGAT GCTGTTCGGA AGGCCTATAA GTATCTAGCT 
GCTCTGCACG AGAACTGCAG CCAGCTCATC CAGACCATCG AGGACACAGG CACCATCATG 
CGGGAGGTTC GAGACCTCGA GGAGCAGATC GAGACAGAGC TGGGCAAGAA GACCCTCAGC 
AACCTGGAGA AGATCCGGGA GGACTACCGA GCCCTCCGCC AGGAGAACGC TGGCCTCCTA 
GGCCGGGTCC GGGAGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CCDC22 ( XM_005272599.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005272656.1 Homo sapiens coiled-coil domain containing 22 (CCDC22), transcript variant X1, mRNA.

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CloneID	OHu53992
Clone ID Related Accession (Same CDS sequence)	XM_005272599.2 , XM_005272599.3 , XM_005272599.4
Accession Version	XM_005272599.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	coiled-coil domain-containing protein 22 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005272599.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG CGGACCGAAT CCTCATCCAT TCGCTGCGCC AGGCCGGCAC GGCAGTTCCT 
CCAGATGTGC AGACCTTGCG CGCCTTCACC ACTGAGCTGG TTGTAGAGGC TGTGGTCCGC 
TGCCTGCGTG TGATCAACCC TGCGGTGGGC TCTGGCCTCA GCCCTCTGCT GCCTCTTGCC 
ATGTCTGCCC GGTTCCGCCT GGCCATGAGC CTGGCTCAGG CCTGCATGGA CCTGGGCTAT 
CCCTTGGAGC TTGGCTATCA GAACTTCCTC TACCCCAGTG AGCCTGACCT CCGAGACCTG 
CTTCTCTTCT TGGCTGAGCG TCTGCCCACC GATGCCTCTG AGGATGCAGA CCAGCCTGCA 
GGTGACTCAG CTATTCTCCT CCGGGCCATT GGGAGCCAAA TTCGGGACCA GCTGGCACTG 
CCTTGGGTCC CGCCCCACCT TCGCACTCCC AAGCTGCAGC ACCTCCAGGG CTCGGCCCTC 
CAGAAGCCTT TCCATGCCAG CAGGCTGGTC GTGCCAGAAT TGAGTTCCAG AGAGCCACGG 
GAGTTCCAGG CGAGTCCCCT GCTGCTTCCA GTCCCTACCC AGGTGCCTCA GCCTGTTGGA 
AGGGTGGCCT CGCTCCTCGA ACACCATGCC CTGCAGCTCT GCCAGCAGAC GGGCCGGGAC 
CGGCCAGGGG ATGAGGACTG GGTCCACCGG ACATCCCGCC TCCCACCCCA GGAGGACACA 
CGGGCTCAGC GGCAGCGGCT GCAAAAGCAA CTGACTGAGC ATCTGCGCCA AAGCTGGGGC 
CTGCTTGGGG CCCCCATACA AGCCCGGGAC CTGGGAGAAC TGCTGCAGGC CTGGGGTGCT 
GGGGCCAAGA CTGGTGCTCC TAAGGGCTCC CGCTTCACGC ACTCAGAGAA GTTCACCTTC 
CATCTGGAGC CCCAGGCCCA GGCCACTCAG GTGTCAGATG TGCCAGCCAC CTCCCGGCGG 
CCTGAACAGG TCACGTGGGC AGCTCAGGAA CAGGAGCTCG AGTCCCTTCG GGAGCAGCTG 
GAAGGAGTGA ACCGCAGCAT TGAGGAGGTT GAGGCCGACA TGAAGACCCT GGGCGTCAGC 
TTTGTGCAGG CAGAGTCTGA GTGCCGGCAC AGCAAGCTCA GTACAGCAGA GCGTGAGCAG 
GCCCTGCGCC TGAAGAGCCG CGCGGTGGAG CTGCTGCCCG ATGGGACTGC CAACCTTGCC 
AAGCTGCAGC TTGTGGTGGA GAATAGTGCC CAGCGGGTCA TCCACTTGGC GGGTCAGTGG 
GAGAAGCACC GGGTCCCACT CCTCGCTGAG TACCGCCACC TCCGAAAGCT GCAGGATTGC 
AGAGAGCTGG AATCTTCTCG ACGGCTGGCA GAGATCCAAG AACTGCACCA GAGTGTCCGG 
GCGGCTGCTG AAGAGGCCCG CAGGAAGGAG GAGGTCTATA AGCAGCTGAT GTCAGAGCTG 
GAGACTCTGC CCAGAGATGT GTCCCGGCTG GCCTACACCC AGCGCATCCT GGAGATCGTG 
GGCAACATCC GGAAGCAGAA GGAAGAGATC ACCAAGATCT TGTCTGATAC GAAGGAGCTT 
CAGAAGGAAA TCAACTCCCT ATCTGGGAAG CTGGACCGGA CGTTTGCGGT GACTGATGAG 
CTTGTGTTCA AGGATGCCAA GAAGGACGAT GCTGTTCGGA AGGCCTATAA GTATCTAGCT 
GCTCTGCACG AGAACTGCAG CCAGCTCATC CAGACCATCG AGGACACAGG CACCATCATG 
CGGGAGGTTC GAGACCTCGA GGAGCAGATC GAGACAGAGC TGGGCAAGAA GACCCTCAGC 
AACCTGGAGA AGATCCGGGA GGACTACCGA GCCCTCCGCC AGGAGAACGC TGGCCTCCTA 
GGCCGGGTCC GGGAGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005272656.1
CDS	103..1983
Translation	MEEADRILIHSLRQAGTAVPPDVQTLRAFTTELVVEAVVRCLRVINPAVGSGLSPLLPLAMSARFRLAMSLAQACMDLGYPLELGYQNFLYPSEPDLRDLLLFLAERLPTDASEDADQPAGDSAILLRAIGSQIRDQLALPWVPPHLRTPKLQHLQGSALQKPFHASRLVVPELSSREPREFQASPLLLPVPTQVPQPVGRVASLLEHHALQLCQQTGRDRPGDEDWVHRTSRLPPQEDTRAQRQRLQKQLTEHLRQSWGLLGAPIQARDLGELLQAWGAGAKTGAPKGSRFTHSEKFTFHLEPQAQATQVSDVPATSRRPEQVTWAAQEQELESLREQLEGVNRSIEEVEADMKTLGVSFVQAESECRHSKLSTAEREQALRLKSRAVELLPDGTANLAKLQLVVENSAQRVIHLAGQWEKHRVPLLAEYRHLRKLQDCRELESSRRLAEIQELHQSVRAAAEEARRKEEVYKQLMSELETLPRDVSRLAYTQRILEIVGNIRKQKEEITKILSDTKELQKEINSLSGKLDRTFAVTDELVFKDAKKDDAVRKAYKYLAALHENCSQLIQTIEDTGTIMREVRDLEEQIETELGKKTLSNLEKIREDYRALRQENAGLLGRVREA

Target ORF information:

RefSeq Version	XM_005272599.3
Organism	Homo sapiens(human)
Definition	Homo sapiens coiled-coil domain containing 22 (CCDC22), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005272599.3

ORF Insert Sequence:

ATGGAGGAGG CGGACCGAAT CCTCATCCAT TCGCTGCGCC AGGCCGGCAC GGCAGTTCCT 
CCAGATGTGC AGACCTTGCG CGCCTTCACC ACTGAGCTGG TTGTAGAGGC TGTGGTCCGC 
TGCCTGCGTG TGATCAACCC TGCGGTGGGC TCTGGCCTCA GCCCTCTGCT GCCTCTTGCC 
ATGTCTGCCC GGTTCCGCCT GGCCATGAGC CTGGCTCAGG CCTGCATGGA CCTGGGCTAT 
CCCTTGGAGC TTGGCTATCA GAACTTCCTC TACCCCAGTG AGCCTGACCT CCGAGACCTG 
CTTCTCTTCT TGGCTGAGCG TCTGCCCACC GATGCCTCTG AGGATGCAGA CCAGCCTGCA 
GGTGACTCAG CTATTCTCCT CCGGGCCATT GGGAGCCAAA TTCGGGACCA GCTGGCACTG 
CCTTGGGTCC CGCCCCACCT TCGCACTCCC AAGCTGCAGC ACCTCCAGGG CTCGGCCCTC 
CAGAAGCCTT TCCATGCCAG CAGGCTGGTC GTGCCAGAAT TGAGTTCCAG AGAGCCACGG 
GAGTTCCAGG CGAGTCCCCT GCTGCTTCCA GTCCCTACCC AGGTGCCTCA GCCTGTTGGA 
AGGGTGGCCT CGCTCCTCGA ACACCATGCC CTGCAGCTCT GCCAGCAGAC GGGCCGGGAC 
CGGCCAGGGG ATGAGGACTG GGTCCACCGG ACATCCCGCC TCCCACCCCA GGAGGACACA 
CGGGCTCAGC GGCAGCGGCT GCAAAAGCAA CTGACTGAGC ATCTGCGCCA AAGCTGGGGC 
CTGCTTGGGG CCCCCATACA AGCCCGGGAC CTGGGAGAAC TGCTGCAGGC CTGGGGTGCT 
GGGGCCAAGA CTGGTGCTCC TAAGGGCTCC CGCTTCACGC ACTCAGAGAA GTTCACCTTC 
CATCTGGAGC CCCAGGCCCA GGCCACTCAG GTGTCAGATG TGCCAGCCAC CTCCCGGCGG 
CCTGAACAGG TCACGTGGGC AGCTCAGGAA CAGGAGCTCG AGTCCCTTCG GGAGCAGCTG 
GAAGGAGTGA ACCGCAGCAT TGAGGAGGTT GAGGCCGACA TGAAGACCCT GGGCGTCAGC 
TTTGTGCAGG CAGAGTCTGA GTGCCGGCAC AGCAAGCTCA GTACAGCAGA GCGTGAGCAG 
GCCCTGCGCC TGAAGAGCCG CGCGGTGGAG CTGCTGCCCG ATGGGACTGC CAACCTTGCC 
AAGCTGCAGC TTGTGGTGGA GAATAGTGCC CAGCGGGTCA TCCACTTGGC GGGTCAGTGG 
GAGAAGCACC GGGTCCCACT CCTCGCTGAG TACCGCCACC TCCGAAAGCT GCAGGATTGC 
AGAGAGCTGG AATCTTCTCG ACGGCTGGCA GAGATCCAAG AACTGCACCA GAGTGTCCGG 
GCGGCTGCTG AAGAGGCCCG CAGGAAGGAG GAGGTCTATA AGCAGCTGAT GTCAGAGCTG 
GAGACTCTGC CCAGAGATGT GTCCCGGCTG GCCTACACCC AGCGCATCCT GGAGATCGTG 
GGCAACATCC GGAAGCAGAA GGAAGAGATC ACCAAGATCT TGTCTGATAC GAAGGAGCTT 
CAGAAGGAAA TCAACTCCCT ATCTGGGAAG CTGGACCGGA CGTTTGCGGT GACTGATGAG 
CTTGTGTTCA AGGATGCCAA GAAGGACGAT GCTGTTCGGA AGGCCTATAA GTATCTAGCT 
GCTCTGCACG AGAACTGCAG CCAGCTCATC CAGACCATCG AGGACACAGG CACCATCATG 
CGGGAGGTTC GAGACCTCGA GGAGCAGATC GAGACAGAGC TGGGCAAGAA GACCCTCAGC 
AACCTGGAGA AGATCCGGGA GGACTACCGA GCCCTCCGCC AGGAGAACGC TGGCCTCCTA 
GGCCGGGTCC GGGAGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CCDC22 ( XM_005272599.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005272656.1 Homo sapiens coiled-coil domain containing 22 (CCDC22), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu53992
Clone ID Related Accession (Same CDS sequence)	XM_005272599.2 , XM_005272599.3 , XM_005272599.4
Accession Version	XM_005272599.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	coiled-coil domain-containing protein 22 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005272599.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG CGGACCGAAT CCTCATCCAT TCGCTGCGCC AGGCCGGCAC GGCAGTTCCT 
CCAGATGTGC AGACCTTGCG CGCCTTCACC ACTGAGCTGG TTGTAGAGGC TGTGGTCCGC 
TGCCTGCGTG TGATCAACCC TGCGGTGGGC TCTGGCCTCA GCCCTCTGCT GCCTCTTGCC 
ATGTCTGCCC GGTTCCGCCT GGCCATGAGC CTGGCTCAGG CCTGCATGGA CCTGGGCTAT 
CCCTTGGAGC TTGGCTATCA GAACTTCCTC TACCCCAGTG AGCCTGACCT CCGAGACCTG 
CTTCTCTTCT TGGCTGAGCG TCTGCCCACC GATGCCTCTG AGGATGCAGA CCAGCCTGCA 
GGTGACTCAG CTATTCTCCT CCGGGCCATT GGGAGCCAAA TTCGGGACCA GCTGGCACTG 
CCTTGGGTCC CGCCCCACCT TCGCACTCCC AAGCTGCAGC ACCTCCAGGG CTCGGCCCTC 
CAGAAGCCTT TCCATGCCAG CAGGCTGGTC GTGCCAGAAT TGAGTTCCAG AGAGCCACGG 
GAGTTCCAGG CGAGTCCCCT GCTGCTTCCA GTCCCTACCC AGGTGCCTCA GCCTGTTGGA 
AGGGTGGCCT CGCTCCTCGA ACACCATGCC CTGCAGCTCT GCCAGCAGAC GGGCCGGGAC 
CGGCCAGGGG ATGAGGACTG GGTCCACCGG ACATCCCGCC TCCCACCCCA GGAGGACACA 
CGGGCTCAGC GGCAGCGGCT GCAAAAGCAA CTGACTGAGC ATCTGCGCCA AAGCTGGGGC 
CTGCTTGGGG CCCCCATACA AGCCCGGGAC CTGGGAGAAC TGCTGCAGGC CTGGGGTGCT 
GGGGCCAAGA CTGGTGCTCC TAAGGGCTCC CGCTTCACGC ACTCAGAGAA GTTCACCTTC 
CATCTGGAGC CCCAGGCCCA GGCCACTCAG GTGTCAGATG TGCCAGCCAC CTCCCGGCGG 
CCTGAACAGG TCACGTGGGC AGCTCAGGAA CAGGAGCTCG AGTCCCTTCG GGAGCAGCTG 
GAAGGAGTGA ACCGCAGCAT TGAGGAGGTT GAGGCCGACA TGAAGACCCT GGGCGTCAGC 
TTTGTGCAGG CAGAGTCTGA GTGCCGGCAC AGCAAGCTCA GTACAGCAGA GCGTGAGCAG 
GCCCTGCGCC TGAAGAGCCG CGCGGTGGAG CTGCTGCCCG ATGGGACTGC CAACCTTGCC 
AAGCTGCAGC TTGTGGTGGA GAATAGTGCC CAGCGGGTCA TCCACTTGGC GGGTCAGTGG 
GAGAAGCACC GGGTCCCACT CCTCGCTGAG TACCGCCACC TCCGAAAGCT GCAGGATTGC 
AGAGAGCTGG AATCTTCTCG ACGGCTGGCA GAGATCCAAG AACTGCACCA GAGTGTCCGG 
GCGGCTGCTG AAGAGGCCCG CAGGAAGGAG GAGGTCTATA AGCAGCTGAT GTCAGAGCTG 
GAGACTCTGC CCAGAGATGT GTCCCGGCTG GCCTACACCC AGCGCATCCT GGAGATCGTG 
GGCAACATCC GGAAGCAGAA GGAAGAGATC ACCAAGATCT TGTCTGATAC GAAGGAGCTT 
CAGAAGGAAA TCAACTCCCT ATCTGGGAAG CTGGACCGGA CGTTTGCGGT GACTGATGAG 
CTTGTGTTCA AGGATGCCAA GAAGGACGAT GCTGTTCGGA AGGCCTATAA GTATCTAGCT 
GCTCTGCACG AGAACTGCAG CCAGCTCATC CAGACCATCG AGGACACAGG CACCATCATG 
CGGGAGGTTC GAGACCTCGA GGAGCAGATC GAGACAGAGC TGGGCAAGAA GACCCTCAGC 
AACCTGGAGA AGATCCGGGA GGACTACCGA GCCCTCCGCC AGGAGAACGC TGGCCTCCTA 
GGCCGGGTCC GGGAGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005272656.1
CDS	116..1996
Translation	MEEADRILIHSLRQAGTAVPPDVQTLRAFTTELVVEAVVRCLRVINPAVGSGLSPLLPLAMSARFRLAMSLAQACMDLGYPLELGYQNFLYPSEPDLRDLLLFLAERLPTDASEDADQPAGDSAILLRAIGSQIRDQLALPWVPPHLRTPKLQHLQGSALQKPFHASRLVVPELSSREPREFQASPLLLPVPTQVPQPVGRVASLLEHHALQLCQQTGRDRPGDEDWVHRTSRLPPQEDTRAQRQRLQKQLTEHLRQSWGLLGAPIQARDLGELLQAWGAGAKTGAPKGSRFTHSEKFTFHLEPQAQATQVSDVPATSRRPEQVTWAAQEQELESLREQLEGVNRSIEEVEADMKTLGVSFVQAESECRHSKLSTAEREQALRLKSRAVELLPDGTANLAKLQLVVENSAQRVIHLAGQWEKHRVPLLAEYRHLRKLQDCRELESSRRLAEIQELHQSVRAAAEEARRKEEVYKQLMSELETLPRDVSRLAYTQRILEIVGNIRKQKEEITKILSDTKELQKEINSLSGKLDRTFAVTDELVFKDAKKDDAVRKAYKYLAALHENCSQLIQTIEDTGTIMREVRDLEEQIETELGKKTLSNLEKIREDYRALRQENAGLLGRVREA

Target ORF information:

RefSeq Version	XM_005272599.4
Organism	Homo sapiens(human)
Definition	Homo sapiens coiled-coil domain containing 22 (CCDC22), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005272599.4

ORF Insert Sequence:

ATGGAGGAGG CGGACCGAAT CCTCATCCAT TCGCTGCGCC AGGCCGGCAC GGCAGTTCCT 
CCAGATGTGC AGACCTTGCG CGCCTTCACC ACTGAGCTGG TTGTAGAGGC TGTGGTCCGC 
TGCCTGCGTG TGATCAACCC TGCGGTGGGC TCTGGCCTCA GCCCTCTGCT GCCTCTTGCC 
ATGTCTGCCC GGTTCCGCCT GGCCATGAGC CTGGCTCAGG CCTGCATGGA CCTGGGCTAT 
CCCTTGGAGC TTGGCTATCA GAACTTCCTC TACCCCAGTG AGCCTGACCT CCGAGACCTG 
CTTCTCTTCT TGGCTGAGCG TCTGCCCACC GATGCCTCTG AGGATGCAGA CCAGCCTGCA 
GGTGACTCAG CTATTCTCCT CCGGGCCATT GGGAGCCAAA TTCGGGACCA GCTGGCACTG 
CCTTGGGTCC CGCCCCACCT TCGCACTCCC AAGCTGCAGC ACCTCCAGGG CTCGGCCCTC 
CAGAAGCCTT TCCATGCCAG CAGGCTGGTC GTGCCAGAAT TGAGTTCCAG AGAGCCACGG 
GAGTTCCAGG CGAGTCCCCT GCTGCTTCCA GTCCCTACCC AGGTGCCTCA GCCTGTTGGA 
AGGGTGGCCT CGCTCCTCGA ACACCATGCC CTGCAGCTCT GCCAGCAGAC GGGCCGGGAC 
CGGCCAGGGG ATGAGGACTG GGTCCACCGG ACATCCCGCC TCCCACCCCA GGAGGACACA 
CGGGCTCAGC GGCAGCGGCT GCAAAAGCAA CTGACTGAGC ATCTGCGCCA AAGCTGGGGC 
CTGCTTGGGG CCCCCATACA AGCCCGGGAC CTGGGAGAAC TGCTGCAGGC CTGGGGTGCT 
GGGGCCAAGA CTGGTGCTCC TAAGGGCTCC CGCTTCACGC ACTCAGAGAA GTTCACCTTC 
CATCTGGAGC CCCAGGCCCA GGCCACTCAG GTGTCAGATG TGCCAGCCAC CTCCCGGCGG 
CCTGAACAGG TCACGTGGGC AGCTCAGGAA CAGGAGCTCG AGTCCCTTCG GGAGCAGCTG 
GAAGGAGTGA ACCGCAGCAT TGAGGAGGTT GAGGCCGACA TGAAGACCCT GGGCGTCAGC 
TTTGTGCAGG CAGAGTCTGA GTGCCGGCAC AGCAAGCTCA GTACAGCAGA GCGTGAGCAG 
GCCCTGCGCC TGAAGAGCCG CGCGGTGGAG CTGCTGCCCG ATGGGACTGC CAACCTTGCC 
AAGCTGCAGC TTGTGGTGGA GAATAGTGCC CAGCGGGTCA TCCACTTGGC GGGTCAGTGG 
GAGAAGCACC GGGTCCCACT CCTCGCTGAG TACCGCCACC TCCGAAAGCT GCAGGATTGC 
AGAGAGCTGG AATCTTCTCG ACGGCTGGCA GAGATCCAAG AACTGCACCA GAGTGTCCGG 
GCGGCTGCTG AAGAGGCCCG CAGGAAGGAG GAGGTCTATA AGCAGCTGAT GTCAGAGCTG 
GAGACTCTGC CCAGAGATGT GTCCCGGCTG GCCTACACCC AGCGCATCCT GGAGATCGTG 
GGCAACATCC GGAAGCAGAA GGAAGAGATC ACCAAGATCT TGTCTGATAC GAAGGAGCTT 
CAGAAGGAAA TCAACTCCCT ATCTGGGAAG CTGGACCGGA CGTTTGCGGT GACTGATGAG 
CTTGTGTTCA AGGATGCCAA GAAGGACGAT GCTGTTCGGA AGGCCTATAA GTATCTAGCT 
GCTCTGCACG AGAACTGCAG CCAGCTCATC CAGACCATCG AGGACACAGG CACCATCATG 
CGGGAGGTTC GAGACCTCGA GGAGCAGATC GAGACAGAGC TGGGCAAGAA GACCCTCAGC 
AACCTGGAGA AGATCCGGGA GGACTACCGA GCCCTCCGCC AGGAGAACGC TGGCCTCCTA 
GGCCGGGTCC GGGAGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling.
Nature cell biology19(10)1214-1225(2017 Oct)
McNally KE,Faulkner R,Steinberg F,Gallon M,Ghai R,Pim D,Langton P,Pearson N,Danson CM,N?gele H,Morris LL,Singla A,Overlee BL,Heesom KJ,Sessions R,Banks L,Collins BM,Berger I,Billadeau DD,Burstein E,Cullen PJ

CCDC22 gene polymorphism is associated with advanced stages of endometriosis in a sample of Brazilian women.
Journal of assisted reproduction and genetics34(7)939-944(2017 Jul)
de Oliveira Francisco D,de Paula Andres M,Gueuvoghlanian-Silva BY,Podgaec S,Fridman C

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Association between rs2294020 in X-linked CCDC22 and susceptibility to autoimmune diseases with focus on systemic lupus erythematosus.
Immunology letters18158-62(2017 Jan)
D'Amico F,Skarmoutsou E,Lo LJ,Granata M,Trovato C,Rossi GA,Bellocchi C,Marchini M,Scorza R,Mazzarino MC,Keinan A

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CCDC22 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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