BEST4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	BEST4
Entrez Gene ID	266675
Full Name	bestrophin 4
Synonyms	VMD2L2
General protein information	Preferred Names bestrophin 4 Names bestrophin-4 vitelliform macular dystrophy 2-like 2 vitelliform macular dystrophy 2-like protein 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p34.1
Summary	This gene is a member of the bestrophin gene family of anion channels. Bestrophin genes share a similar gene structure with highly conserved exon-intron boundaries, but with distinct 3' ends. Bestrophins are transmembrane proteins that contain a homologous region rich in aromatic residues, including an invariant arg-phe-pro motif. Mutation in one of the family members (bestrophin 1) is associated with vitelliform macular dystrophy. The bestrophin 4 gene is predominantly expressed in the colon. [provided by RefSeq, Jul 2008].
Disorder MIM:	607336

mRNA and Protein(s)

mRNA	Protein	Name
XM_017001022.1	XP_016856511.1	bestrophin-4 isoform X1
XM_017001020.1	XP_016856509.1	bestrophin-4 isoform X1
NM_153274.2	NP_695006.1	bestrophin-4
NM_153274.3	NP_695006.1	bestrophin-4
XM_024446367.1	XP_024302135.1	bestrophin-4 isoform X1
XM_017001021.1	XP_016856510.1	bestrophin-4 isoform X1
XM_017001023.1	XP_016856512.1	bestrophin-4 isoform X1

Pathways from BioSystems

Homologies

	BEST4	XP_001098771.1
Canis lupus familiaris (dog)	BEST4	XP_539638.1
Bos taurus (cattle)	BEST4	NP_001192608.1
Xenopus tropicalis (tropical clawed frog)	best4	NP_001116914.1
Homo sapiens (human)	BEST4	NP_695006.1
Pan troglodytes (chimpanzee)	BEST4	XP_524571.2
Rattus norvegicus (Norway rat)	Best4	XP_001066317.1
Gallus gallus (chicken)	BEST4	NP_001239054.1
Danio rerio (zebrafish)	best4	XP_692160.3
Drosophila melanogaster (fruit fly)	Best4	NP_001097609.1

Gene Ontology
Bibliography

Related articles in PubMed

Lineage-specific expression of bestrophin-2 and bestrophin-4 in human intestinal epithelial cells.
Ito G, Okamoto R, Murano T, Shimizu H, Fujii S, Nakata T, Mizutani T, Yui S, Akiyama-Morio J, Nemoto Y, Okada E, Araki A, Ohtsuka K, Tsuchiya K, Nakamura T, Watanabe M
PloS one8(11)e79693(2013)

Bestrophin expression and function in the human pancreatic duct cell line, CFPAC-1.
Marsey LL, Winpenny JP
The Journal of physiology587(Pt 10)2211-24(2009 May)

Ca2+-activated Cl- current from human bestrophin-4 in excised membrane patches.
Tsunenari T, Nathans J, Yau KW
The Journal of general physiology127(6)749-54(2006 Jun)

Structure-function analysis of the bestrophin family of anion channels.
Tsunenari T, Sun H, Williams J, Cahill H, Smallwood P, Yau KW, Nathans J
The Journal of biological chemistry278(42)41114-25(2003 Oct)

Three novel human VMD2-like genes are members of the evolutionary highly conserved RFP-TM family.
St?hr H, Marquardt A, Nanda I, Schmid M, Weber BH
European journal of human genetics : EJHG10(4)281-4(2002 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

BEST2 and BEST4 could be added to the lineage-specific genes of humans intestinal epithelial cells
Title: Lineage-specific expression of bestrophin-2 and bestrophin-4 in human intestinal epithelial cells.

Results provide evidence that the bestrophins are expressed in pancreatic duct cells and, more specifically, that hBest1 plays a role in the calcium activated chloride channels found in these cells.
Title: Bestrophin expression and function in the human pancreatic duct cell line, CFPAC-1.

Human Best4 chloride channels on excised membrane patches can be activated by free calcium (Ca2+) on the cytoplasmic side.
Title: Ca2+-activated Cl- current from human bestrophin-4 in excised membrane patches.

identified three novel VMD2-related human genes demonstrating a high degree of conservation in their respective RFP-TM domains
Title: Three novel human VMD2-like genes are members of the evolutionary highly conserved RFP-TM family.

The following BEST4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BEST4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06993	NM_153274.3 Latest version!	Homo sapiens bestrophin 4 (BEST4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu06993	XM_017001022.1 Latest version!	Homo sapiens bestrophin 4 (BEST4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu06993	XM_024446367.1 Latest version!	Homo sapiens bestrophin 4 (BEST4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu06993	XM_017001023.1 Latest version!	Homo sapiens bestrophin 4 (BEST4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu87249	XM_017001020.1 Latest version!	Homo sapiens bestrophin 4 (BEST4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu87249	XM_017001021.1 Latest version!	Homo sapiens bestrophin 4 (BEST4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu06993	NM_153274.2	Homo sapiens bestrophin 4 (BEST4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BEST4 ( NM_153274.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_695006.1 Homo sapiens bestrophin 4 (BEST4), mRNA.

Price & Availability↑

CloneID	OHu06993
Clone ID Related Accession (Same CDS sequence)	XM_017001022.1 , NM_153274.2 , NM_153274.3 , XM_024446367.1 , XM_017001023.1
Accession Version	NM_153274.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	bestrophin-4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC101823.1, AF440757.1 and BM142321.1. On Dec 27, 2006 this sequence version replaced NM_153274.1. ##Evidence-Data-START## Transcript exon combination :: BC101823.1, AF440757.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGTTT CATACACTCT CAAAGTGGCG GAGGCCCGCT TCGGAGGTTT CTCTGGCCTG 
CTTCTCCGCT GGAGGGGAAG CATCTACAAG CTCCTCTACA AGGAATTCCT CCTCTTTGGG 
GCCTTGTACG CTGTGCTTAG CATCACCTAC CGGCTGCTGC TGACCCAGGA GCAGAGGTAC 
GTGTATGCTC AGGTGGCCCG GTACTGCAAC CGCTCAGCAG ACCTCATTCC CTTGTCCTTT 
GTATTGGGTT TCTATGTGAC TCTCGTGGTG AACCGCTGGT GGTCCCAGTA CACAAGCATC 
CCGCTGCCAG ACCAGCTGAT GTGCGTCATC TCGGCTAGCG TGCACGGCGT GGACCAGCGG 
GGCCGCCTGC TGCGCCGCAC CCTCATCCGC TACGCGAACC TGGCGTCCGT GCTGGTGCTG 
CGCTCGGTCA GCACCCGCGT GCTTAAGCGC TTCCCCACCA TGGAGCACGT GGTGGACGCA 
GGTTTCATGT CCCAGGAAGA GAGGAAAAAG TTTGAGAGCC TGAAATCCGA CTTCAACAAG 
TACTGGGTCC CCTGCGTCTG GTTCACCAAC CTGGCGGCCC AGGCCCGGAG GGACGGGCGA 
ATACGTGACG ATATCGCTCT CTGTCTACTT TTGGAAGAGC TGAACAAGTA CCGAGCCAAG 
TGCAGCATGC TATTCCACTA TGACTGGATC AGCATCCCCC TCGTCTACAC CCAAGTGGTG 
ACCATAGCCG TCTACTCTTT CTTTGCCCTC TCCCTGGTTG GCCGCCAGTT TGTGGAGCCA 
GAGGCAGGGG CTGCCAAACC TCAGAAGCTT CTGAAGCCAG GCCAGGAGCC AGCCCCAGCC 
CTGGGAGACC CGGACATGTA CGTGCCTCTC ACCACTCTGC TGCAGTTCTT CTTCTATGCT 
GGCTGGCTCA AGGTGGCTGA ACAGATCATC AACCCATTTG GTGAGGATGA TGACGACTTT 
GAGACAAATC AGCTCATAGA CCGCAACTTG CAGGTGTCCC TGCTATCCGT GGACGAAATG 
TACCAGAACC TTCCCCCCGC TGAGAAGGAC CAGTACTGGG ATGAGGACCA GCCGCAGCCA 
CCCTACACTG TGGCCACGGC GGCCGAGTCT CTGCGGCCCT CATTCCTGGG CTCCACCTTC 
AACCTGCGCA TGAGCGACGA CCCTGAGCAG AGCCTGCAGG TGGAGGCGTC CCCCGGATCT 
GGTCGGCCCG CGCCCGCCGC GCAGACCCCG TTGCTCGGCC GCTTCCTGGG CGTAGGGGCG 
CCCTCCCCGG CCATCAGCCT CCGGAACTTC GGCCGCGTGC GAGGCACCCC CCGCCCCCCG 
CATCTGCTGC GCTTCCGGGC GGAGGAGGGC GGCGACCCCG AGGCCGCAGC CCGCATCGAG 
GAGGAATCGG CGGAGTCCGG GGACGAGGCC CTGGAGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_695006.1
CDS	50..1471
Translation	MTVSYTLKVAEARFGGFSGLLLRWRGSIYKLLYKEFLLFGALYAVLSITYRLLLTQEQRYVYAQVARYCNRSADLIPLSFVLGFYVTLVVNRWWSQYTSIPLPDQLMCVISASVHGVDQRGRLLRRTLIRYANLASVLVLRSVSTRVLKRFPTMEHVVDAGFMSQEERKKFESLKSDFNKYWVPCVWFTNLAAQARRDGRIRDDIALCLLLEELNKYRAKCSMLFHYDWISIPLVYTQVVTIAVYSFFALSLVGRQFVEPEAGAAKPQKLLKPGQEPAPALGDPDMYVPLTTLLQFFFYAGWLKVAEQIINPFGEDDDDFETNQLIDRNLQVSLLSVDEMYQNLPPAEKDQYWDEDQPQPPYTVATAAESLRPSFLGSTFNLRMSDDPEQSLQVEASPGSGRPAPAAQTPLLGRFLGVGAPSPAISLRNFGRVRGTPRPPHLLRFRAEEGGDPEAAARIEEESAESGDEALEP

Target ORF information:

RefSeq Version	NM_153274.2
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 4 (BEST4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153274.2

ORF Insert Sequence:

ATGACGGTTT CATACACTCT CAAAGTGGCG GAGGCCCGCT TCGGAGGTTT CTCTGGCCTG 
CTTCTCCGCT GGAGGGGAAG CATCTACAAG CTCCTCTACA AGGAATTCCT CCTCTTTGGG 
GCCTTGTACG CTGTGCTTAG CATCACCTAC CGGCTGCTGC TGACCCAGGA GCAGAGGTAC 
GTGTATGCTC AGGTGGCCCG GTACTGCAAC CGCTCAGCAG ACCTCATTCC CTTGTCCTTT 
GTATTGGGTT TCTATGTGAC TCTCGTGGTG AACCGCTGGT GGTCCCAGTA CACAAGCATC 
CCGCTGCCAG ACCAGCTGAT GTGCGTCATC TCGGCTAGCG TGCACGGCGT GGACCAGCGG 
GGCCGCCTGC TGCGCCGCAC CCTCATCCGC TACGCGAACC TGGCGTCCGT GCTGGTGCTG 
CGCTCGGTCA GCACCCGCGT GCTTAAGCGC TTCCCCACCA TGGAGCACGT GGTGGACGCA 
GGTTTCATGT CCCAGGAAGA GAGGAAAAAG TTTGAGAGCC TGAAATCCGA CTTCAACAAG 
TACTGGGTCC CCTGCGTCTG GTTCACCAAC CTGGCGGCCC AGGCCCGGAG GGACGGGCGA 
ATACGTGACG ATATCGCTCT CTGTCTACTT TTGGAAGAGC TGAACAAGTA CCGAGCCAAG 
TGCAGCATGC TATTCCACTA TGACTGGATC AGCATCCCCC TCGTCTACAC CCAAGTGGTG 
ACCATAGCCG TCTACTCTTT CTTTGCCCTC TCCCTGGTTG GCCGCCAGTT TGTGGAGCCA 
GAGGCAGGGG CTGCCAAACC TCAGAAGCTT CTGAAGCCAG GCCAGGAGCC AGCCCCAGCC 
CTGGGAGACC CGGACATGTA CGTGCCTCTC ACCACTCTGC TGCAGTTCTT CTTCTATGCT 
GGCTGGCTCA AGGTGGCTGA ACAGATCATC AACCCATTTG GTGAGGATGA TGACGACTTT 
GAGACAAATC AGCTCATAGA CCGCAACTTG CAGGTGTCCC TGCTATCCGT GGACGAAATG 
TACCAGAACC TTCCCCCCGC TGAGAAGGAC CAGTACTGGG ATGAGGACCA GCCGCAGCCA 
CCCTACACTG TGGCCACGGC GGCCGAGTCT CTGCGGCCCT CATTCCTGGG CTCCACCTTC 
AACCTGCGCA TGAGCGACGA CCCTGAGCAG AGCCTGCAGG TGGAGGCGTC CCCCGGATCT 
GGTCGGCCCG CGCCCGCCGC GCAGACCCCG TTGCTCGGCC GCTTCCTGGG CGTAGGGGCG 
CCCTCCCCGG CCATCAGCCT CCGGAACTTC GGCCGCGTGC GAGGCACCCC CCGCCCCCCG 
CATCTGCTGC GCTTCCGGGC GGAGGAGGGC GGCGACCCCG AGGCCGCAGC CCGCATCGAG 
GAGGAATCGG CGGAGTCCGG GGACGAGGCC CTGGAGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST4 ( NM_153274.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_695006.1 Homo sapiens bestrophin 4 (BEST4), mRNA.

Price & Availability↑

CloneID	OHu06993
Clone ID Related Accession (Same CDS sequence)	XM_017001022.1 , NM_153274.2 , NM_153274.3 , XM_024446367.1 , XM_017001023.1
Accession Version	NM_153274.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	bestrophin-4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL592166.16, BC101823.1, AF440757.1 and BM142321.1. On Nov 23, 2018 this sequence version replaced NM_153274.2. ##Evidence-Data-START## Transcript exon combination :: AF440757.1, BC101823.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000372207.4/ ENSP00000361281.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACGGTTT CATACACTCT CAAAGTGGCG GAGGCCCGCT TCGGAGGTTT CTCTGGCCTG 
CTTCTCCGCT GGAGGGGAAG CATCTACAAG CTCCTCTACA AGGAATTCCT CCTCTTTGGG 
GCCTTGTACG CTGTGCTTAG CATCACCTAC CGGCTGCTGC TGACCCAGGA GCAGAGGTAC 
GTGTATGCTC AGGTGGCCCG GTACTGCAAC CGCTCAGCAG ACCTCATTCC CTTGTCCTTT 
GTATTGGGTT TCTATGTGAC TCTCGTGGTG AACCGCTGGT GGTCCCAGTA CACAAGCATC 
CCGCTGCCAG ACCAGCTGAT GTGCGTCATC TCGGCTAGCG TGCACGGCGT GGACCAGCGG 
GGCCGCCTGC TGCGCCGCAC CCTCATCCGC TACGCGAACC TGGCGTCCGT GCTGGTGCTG 
CGCTCGGTCA GCACCCGCGT GCTTAAGCGC TTCCCCACCA TGGAGCACGT GGTGGACGCA 
GGTTTCATGT CCCAGGAAGA GAGGAAAAAG TTTGAGAGCC TGAAATCCGA CTTCAACAAG 
TACTGGGTCC CCTGCGTCTG GTTCACCAAC CTGGCGGCCC AGGCCCGGAG GGACGGGCGA 
ATACGTGACG ATATCGCTCT CTGTCTACTT TTGGAAGAGC TGAACAAGTA CCGAGCCAAG 
TGCAGCATGC TATTCCACTA TGACTGGATC AGCATCCCCC TCGTCTACAC CCAAGTGGTG 
ACCATAGCCG TCTACTCTTT CTTTGCCCTC TCCCTGGTTG GCCGCCAGTT TGTGGAGCCA 
GAGGCAGGGG CTGCCAAACC TCAGAAGCTT CTGAAGCCAG GCCAGGAGCC AGCCCCAGCC 
CTGGGAGACC CGGACATGTA CGTGCCTCTC ACCACTCTGC TGCAGTTCTT CTTCTATGCT 
GGCTGGCTCA AGGTGGCTGA ACAGATCATC AACCCATTTG GTGAGGATGA TGACGACTTT 
GAGACAAATC AGCTCATAGA CCGCAACTTG CAGGTGTCCC TGCTATCCGT GGACGAAATG 
TACCAGAACC TTCCCCCCGC TGAGAAGGAC CAGTACTGGG ATGAGGACCA GCCGCAGCCA 
CCCTACACTG TGGCCACGGC GGCCGAGTCT CTGCGGCCCT CATTCCTGGG CTCCACCTTC 
AACCTGCGCA TGAGCGACGA CCCTGAGCAG AGCCTGCAGG TGGAGGCGTC CCCCGGATCT 
GGTCGGCCCG CGCCCGCCGC GCAGACCCCG TTGCTCGGCC GCTTCCTGGG CGTAGGGGCG 
CCCTCCCCGG CCATCAGCCT CCGGAACTTC GGCCGCGTGC GAGGCACCCC CCGCCCCCCG 
CATCTGCTGC GCTTCCGGGC GGAGGAGGGC GGCGACCCCG AGGCCGCAGC CCGCATCGAG 
GAGGAATCGG CGGAGTCCGG GGACGAGGCC CTGGAGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_695006.1
CDS	466..1887
Translation	MTVSYTLKVAEARFGGFSGLLLRWRGSIYKLLYKEFLLFGALYAVLSITYRLLLTQEQRYVYAQVARYCNRSADLIPLSFVLGFYVTLVVNRWWSQYTSIPLPDQLMCVISASVHGVDQRGRLLRRTLIRYANLASVLVLRSVSTRVLKRFPTMEHVVDAGFMSQEERKKFESLKSDFNKYWVPCVWFTNLAAQARRDGRIRDDIALCLLLEELNKYRAKCSMLFHYDWISIPLVYTQVVTIAVYSFFALSLVGRQFVEPEAGAAKPQKLLKPGQEPAPALGDPDMYVPLTTLLQFFFYAGWLKVAEQIINPFGEDDDDFETNQLIDRNLQVSLLSVDEMYQNLPPAEKDQYWDEDQPQPPYTVATAAESLRPSFLGSTFNLRMSDDPEQSLQVEASPGSGRPAPAAQTPLLGRFLGVGAPSPAISLRNFGRVRGTPRPPHLLRFRAEEGGDPEAAARIEEESAESGDEALEP

Target ORF information:

RefSeq Version	NM_153274.3
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 4 (BEST4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153274.3

ORF Insert Sequence:

ATGACGGTTT CATACACTCT CAAAGTGGCG GAGGCCCGCT TCGGAGGTTT CTCTGGCCTG 
CTTCTCCGCT GGAGGGGAAG CATCTACAAG CTCCTCTACA AGGAATTCCT CCTCTTTGGG 
GCCTTGTACG CTGTGCTTAG CATCACCTAC CGGCTGCTGC TGACCCAGGA GCAGAGGTAC 
GTGTATGCTC AGGTGGCCCG GTACTGCAAC CGCTCAGCAG ACCTCATTCC CTTGTCCTTT 
GTATTGGGTT TCTATGTGAC TCTCGTGGTG AACCGCTGGT GGTCCCAGTA CACAAGCATC 
CCGCTGCCAG ACCAGCTGAT GTGCGTCATC TCGGCTAGCG TGCACGGCGT GGACCAGCGG 
GGCCGCCTGC TGCGCCGCAC CCTCATCCGC TACGCGAACC TGGCGTCCGT GCTGGTGCTG 
CGCTCGGTCA GCACCCGCGT GCTTAAGCGC TTCCCCACCA TGGAGCACGT GGTGGACGCA 
GGTTTCATGT CCCAGGAAGA GAGGAAAAAG TTTGAGAGCC TGAAATCCGA CTTCAACAAG 
TACTGGGTCC CCTGCGTCTG GTTCACCAAC CTGGCGGCCC AGGCCCGGAG GGACGGGCGA 
ATACGTGACG ATATCGCTCT CTGTCTACTT TTGGAAGAGC TGAACAAGTA CCGAGCCAAG 
TGCAGCATGC TATTCCACTA TGACTGGATC AGCATCCCCC TCGTCTACAC CCAAGTGGTG 
ACCATAGCCG TCTACTCTTT CTTTGCCCTC TCCCTGGTTG GCCGCCAGTT TGTGGAGCCA 
GAGGCAGGGG CTGCCAAACC TCAGAAGCTT CTGAAGCCAG GCCAGGAGCC AGCCCCAGCC 
CTGGGAGACC CGGACATGTA CGTGCCTCTC ACCACTCTGC TGCAGTTCTT CTTCTATGCT 
GGCTGGCTCA AGGTGGCTGA ACAGATCATC AACCCATTTG GTGAGGATGA TGACGACTTT 
GAGACAAATC AGCTCATAGA CCGCAACTTG CAGGTGTCCC TGCTATCCGT GGACGAAATG 
TACCAGAACC TTCCCCCCGC TGAGAAGGAC CAGTACTGGG ATGAGGACCA GCCGCAGCCA 
CCCTACACTG TGGCCACGGC GGCCGAGTCT CTGCGGCCCT CATTCCTGGG CTCCACCTTC 
AACCTGCGCA TGAGCGACGA CCCTGAGCAG AGCCTGCAGG TGGAGGCGTC CCCCGGATCT 
GGTCGGCCCG CGCCCGCCGC GCAGACCCCG TTGCTCGGCC GCTTCCTGGG CGTAGGGGCG 
CCCTCCCCGG CCATCAGCCT CCGGAACTTC GGCCGCGTGC GAGGCACCCC CCGCCCCCCG 
CATCTGCTGC GCTTCCGGGC GGAGGAGGGC GGCGACCCCG AGGCCGCAGC CCGCATCGAG 
GAGGAATCGG CGGAGTCCGG GGACGAGGCC CTGGAGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST4 ( XM_017001022.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856511.1 Homo sapiens bestrophin 4 (BEST4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu06993
Clone ID Related Accession (Same CDS sequence)	XM_017001022.1 , NM_153274.2 , NM_153274.3 , XM_024446367.1 , XM_017001023.1
Accession Version	XM_017001022.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bestrophin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGTTT CATACACTCT CAAAGTGGCG GAGGCCCGCT TCGGAGGTTT CTCTGGCCTG 
CTTCTCCGCT GGAGGGGAAG CATCTACAAG CTCCTCTACA AGGAATTCCT CCTCTTTGGG 
GCCTTGTACG CTGTGCTTAG CATCACCTAC CGGCTGCTGC TGACCCAGGA GCAGAGGTAC 
GTGTATGCTC AGGTGGCCCG GTACTGCAAC CGCTCAGCAG ACCTCATTCC CTTGTCCTTT 
GTATTGGGTT TCTATGTGAC TCTCGTGGTG AACCGCTGGT GGTCCCAGTA CACAAGCATC 
CCGCTGCCAG ACCAGCTGAT GTGCGTCATC TCGGCTAGCG TGCACGGCGT GGACCAGCGG 
GGCCGCCTGC TGCGCCGCAC CCTCATCCGC TACGCGAACC TGGCGTCCGT GCTGGTGCTG 
CGCTCGGTCA GCACCCGCGT GCTTAAGCGC TTCCCCACCA TGGAGCACGT GGTGGACGCA 
GGTTTCATGT CCCAGGAAGA GAGGAAAAAG TTTGAGAGCC TGAAATCCGA CTTCAACAAG 
TACTGGGTCC CCTGCGTCTG GTTCACCAAC CTGGCGGCCC AGGCCCGGAG GGACGGGCGA 
ATACGTGACG ATATCGCTCT CTGTCTACTT TTGGAAGAGC TGAACAAGTA CCGAGCCAAG 
TGCAGCATGC TATTCCACTA TGACTGGATC AGCATCCCCC TCGTCTACAC CCAAGTGGTG 
ACCATAGCCG TCTACTCTTT CTTTGCCCTC TCCCTGGTTG GCCGCCAGTT TGTGGAGCCA 
GAGGCAGGGG CTGCCAAACC TCAGAAGCTT CTGAAGCCAG GCCAGGAGCC AGCCCCAGCC 
CTGGGAGACC CGGACATGTA CGTGCCTCTC ACCACTCTGC TGCAGTTCTT CTTCTATGCT 
GGCTGGCTCA AGGTGGCTGA ACAGATCATC AACCCATTTG GTGAGGATGA TGACGACTTT 
GAGACAAATC AGCTCATAGA CCGCAACTTG CAGGTGTCCC TGCTATCCGT GGACGAAATG 
TACCAGAACC TTCCCCCCGC TGAGAAGGAC CAGTACTGGG ATGAGGACCA GCCGCAGCCA 
CCCTACACTG TGGCCACGGC GGCCGAGTCT CTGCGGCCCT CATTCCTGGG CTCCACCTTC 
AACCTGCGCA TGAGCGACGA CCCTGAGCAG AGCCTGCAGG TGGAGGCGTC CCCCGGATCT 
GGTCGGCCCG CGCCCGCCGC GCAGACCCCG TTGCTCGGCC GCTTCCTGGG CGTAGGGGCG 
CCCTCCCCGG CCATCAGCCT CCGGAACTTC GGCCGCGTGC GAGGCACCCC CCGCCCCCCG 
CATCTGCTGC GCTTCCGGGC GGAGGAGGGC GGCGACCCCG AGGCCGCAGC CCGCATCGAG 
GAGGAATCGG CGGAGTCCGG GGACGAGGCC CTGGAGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856511.1
CDS	1401..2822
Translation	MTVSYTLKVAEARFGGFSGLLLRWRGSIYKLLYKEFLLFGALYAVLSITYRLLLTQEQRYVYAQVARYCNRSADLIPLSFVLGFYVTLVVNRWWSQYTSIPLPDQLMCVISASVHGVDQRGRLLRRTLIRYANLASVLVLRSVSTRVLKRFPTMEHVVDAGFMSQEERKKFESLKSDFNKYWVPCVWFTNLAAQARRDGRIRDDIALCLLLEELNKYRAKCSMLFHYDWISIPLVYTQVVTIAVYSFFALSLVGRQFVEPEAGAAKPQKLLKPGQEPAPALGDPDMYVPLTTLLQFFFYAGWLKVAEQIINPFGEDDDDFETNQLIDRNLQVSLLSVDEMYQNLPPAEKDQYWDEDQPQPPYTVATAAESLRPSFLGSTFNLRMSDDPEQSLQVEASPGSGRPAPAAQTPLLGRFLGVGAPSPAISLRNFGRVRGTPRPPHLLRFRAEEGGDPEAAARIEEESAESGDEALEP

Target ORF information:

RefSeq Version	XM_017001022.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 4 (BEST4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001022.1

ORF Insert Sequence:

ATGACGGTTT CATACACTCT CAAAGTGGCG GAGGCCCGCT TCGGAGGTTT CTCTGGCCTG 
CTTCTCCGCT GGAGGGGAAG CATCTACAAG CTCCTCTACA AGGAATTCCT CCTCTTTGGG 
GCCTTGTACG CTGTGCTTAG CATCACCTAC CGGCTGCTGC TGACCCAGGA GCAGAGGTAC 
GTGTATGCTC AGGTGGCCCG GTACTGCAAC CGCTCAGCAG ACCTCATTCC CTTGTCCTTT 
GTATTGGGTT TCTATGTGAC TCTCGTGGTG AACCGCTGGT GGTCCCAGTA CACAAGCATC 
CCGCTGCCAG ACCAGCTGAT GTGCGTCATC TCGGCTAGCG TGCACGGCGT GGACCAGCGG 
GGCCGCCTGC TGCGCCGCAC CCTCATCCGC TACGCGAACC TGGCGTCCGT GCTGGTGCTG 
CGCTCGGTCA GCACCCGCGT GCTTAAGCGC TTCCCCACCA TGGAGCACGT GGTGGACGCA 
GGTTTCATGT CCCAGGAAGA GAGGAAAAAG TTTGAGAGCC TGAAATCCGA CTTCAACAAG 
TACTGGGTCC CCTGCGTCTG GTTCACCAAC CTGGCGGCCC AGGCCCGGAG GGACGGGCGA 
ATACGTGACG ATATCGCTCT CTGTCTACTT TTGGAAGAGC TGAACAAGTA CCGAGCCAAG 
TGCAGCATGC TATTCCACTA TGACTGGATC AGCATCCCCC TCGTCTACAC CCAAGTGGTG 
ACCATAGCCG TCTACTCTTT CTTTGCCCTC TCCCTGGTTG GCCGCCAGTT TGTGGAGCCA 
GAGGCAGGGG CTGCCAAACC TCAGAAGCTT CTGAAGCCAG GCCAGGAGCC AGCCCCAGCC 
CTGGGAGACC CGGACATGTA CGTGCCTCTC ACCACTCTGC TGCAGTTCTT CTTCTATGCT 
GGCTGGCTCA AGGTGGCTGA ACAGATCATC AACCCATTTG GTGAGGATGA TGACGACTTT 
GAGACAAATC AGCTCATAGA CCGCAACTTG CAGGTGTCCC TGCTATCCGT GGACGAAATG 
TACCAGAACC TTCCCCCCGC TGAGAAGGAC CAGTACTGGG ATGAGGACCA GCCGCAGCCA 
CCCTACACTG TGGCCACGGC GGCCGAGTCT CTGCGGCCCT CATTCCTGGG CTCCACCTTC 
AACCTGCGCA TGAGCGACGA CCCTGAGCAG AGCCTGCAGG TGGAGGCGTC CCCCGGATCT 
GGTCGGCCCG CGCCCGCCGC GCAGACCCCG TTGCTCGGCC GCTTCCTGGG CGTAGGGGCG 
CCCTCCCCGG CCATCAGCCT CCGGAACTTC GGCCGCGTGC GAGGCACCCC CCGCCCCCCG 
CATCTGCTGC GCTTCCGGGC GGAGGAGGGC GGCGACCCCG AGGCCGCAGC CCGCATCGAG 
GAGGAATCGG CGGAGTCCGG GGACGAGGCC CTGGAGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST4 ( XM_024446367.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024302135.1 Homo sapiens bestrophin 4 (BEST4), transcript variant X3, mRNA.

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CloneID	OHu06993
Clone ID Related Accession (Same CDS sequence)	XM_017001022.1 , NM_153274.2 , NM_153274.3 , XM_024446367.1 , XM_017001023.1
Accession Version	XM_024446367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bestrophin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGTTT CATACACTCT CAAAGTGGCG GAGGCCCGCT TCGGAGGTTT CTCTGGCCTG 
CTTCTCCGCT GGAGGGGAAG CATCTACAAG CTCCTCTACA AGGAATTCCT CCTCTTTGGG 
GCCTTGTACG CTGTGCTTAG CATCACCTAC CGGCTGCTGC TGACCCAGGA GCAGAGGTAC 
GTGTATGCTC AGGTGGCCCG GTACTGCAAC CGCTCAGCAG ACCTCATTCC CTTGTCCTTT 
GTATTGGGTT TCTATGTGAC TCTCGTGGTG AACCGCTGGT GGTCCCAGTA CACAAGCATC 
CCGCTGCCAG ACCAGCTGAT GTGCGTCATC TCGGCTAGCG TGCACGGCGT GGACCAGCGG 
GGCCGCCTGC TGCGCCGCAC CCTCATCCGC TACGCGAACC TGGCGTCCGT GCTGGTGCTG 
CGCTCGGTCA GCACCCGCGT GCTTAAGCGC TTCCCCACCA TGGAGCACGT GGTGGACGCA 
GGTTTCATGT CCCAGGAAGA GAGGAAAAAG TTTGAGAGCC TGAAATCCGA CTTCAACAAG 
TACTGGGTCC CCTGCGTCTG GTTCACCAAC CTGGCGGCCC AGGCCCGGAG GGACGGGCGA 
ATACGTGACG ATATCGCTCT CTGTCTACTT TTGGAAGAGC TGAACAAGTA CCGAGCCAAG 
TGCAGCATGC TATTCCACTA TGACTGGATC AGCATCCCCC TCGTCTACAC CCAAGTGGTG 
ACCATAGCCG TCTACTCTTT CTTTGCCCTC TCCCTGGTTG GCCGCCAGTT TGTGGAGCCA 
GAGGCAGGGG CTGCCAAACC TCAGAAGCTT CTGAAGCCAG GCCAGGAGCC AGCCCCAGCC 
CTGGGAGACC CGGACATGTA CGTGCCTCTC ACCACTCTGC TGCAGTTCTT CTTCTATGCT 
GGCTGGCTCA AGGTGGCTGA ACAGATCATC AACCCATTTG GTGAGGATGA TGACGACTTT 
GAGACAAATC AGCTCATAGA CCGCAACTTG CAGGTGTCCC TGCTATCCGT GGACGAAATG 
TACCAGAACC TTCCCCCCGC TGAGAAGGAC CAGTACTGGG ATGAGGACCA GCCGCAGCCA 
CCCTACACTG TGGCCACGGC GGCCGAGTCT CTGCGGCCCT CATTCCTGGG CTCCACCTTC 
AACCTGCGCA TGAGCGACGA CCCTGAGCAG AGCCTGCAGG TGGAGGCGTC CCCCGGATCT 
GGTCGGCCCG CGCCCGCCGC GCAGACCCCG TTGCTCGGCC GCTTCCTGGG CGTAGGGGCG 
CCCTCCCCGG CCATCAGCCT CCGGAACTTC GGCCGCGTGC GAGGCACCCC CCGCCCCCCG 
CATCTGCTGC GCTTCCGGGC GGAGGAGGGC GGCGACCCCG AGGCCGCAGC CCGCATCGAG 
GAGGAATCGG CGGAGTCCGG GGACGAGGCC CTGGAGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024302135.1
CDS	277..1698
Translation	MTVSYTLKVAEARFGGFSGLLLRWRGSIYKLLYKEFLLFGALYAVLSITYRLLLTQEQRYVYAQVARYCNRSADLIPLSFVLGFYVTLVVNRWWSQYTSIPLPDQLMCVISASVHGVDQRGRLLRRTLIRYANLASVLVLRSVSTRVLKRFPTMEHVVDAGFMSQEERKKFESLKSDFNKYWVPCVWFTNLAAQARRDGRIRDDIALCLLLEELNKYRAKCSMLFHYDWISIPLVYTQVVTIAVYSFFALSLVGRQFVEPEAGAAKPQKLLKPGQEPAPALGDPDMYVPLTTLLQFFFYAGWLKVAEQIINPFGEDDDDFETNQLIDRNLQVSLLSVDEMYQNLPPAEKDQYWDEDQPQPPYTVATAAESLRPSFLGSTFNLRMSDDPEQSLQVEASPGSGRPAPAAQTPLLGRFLGVGAPSPAISLRNFGRVRGTPRPPHLLRFRAEEGGDPEAAARIEEESAESGDEALEP

Target ORF information:

RefSeq Version	XM_024446367.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 4 (BEST4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024446367.1

ORF Insert Sequence:

ATGACGGTTT CATACACTCT CAAAGTGGCG GAGGCCCGCT TCGGAGGTTT CTCTGGCCTG 
CTTCTCCGCT GGAGGGGAAG CATCTACAAG CTCCTCTACA AGGAATTCCT CCTCTTTGGG 
GCCTTGTACG CTGTGCTTAG CATCACCTAC CGGCTGCTGC TGACCCAGGA GCAGAGGTAC 
GTGTATGCTC AGGTGGCCCG GTACTGCAAC CGCTCAGCAG ACCTCATTCC CTTGTCCTTT 
GTATTGGGTT TCTATGTGAC TCTCGTGGTG AACCGCTGGT GGTCCCAGTA CACAAGCATC 
CCGCTGCCAG ACCAGCTGAT GTGCGTCATC TCGGCTAGCG TGCACGGCGT GGACCAGCGG 
GGCCGCCTGC TGCGCCGCAC CCTCATCCGC TACGCGAACC TGGCGTCCGT GCTGGTGCTG 
CGCTCGGTCA GCACCCGCGT GCTTAAGCGC TTCCCCACCA TGGAGCACGT GGTGGACGCA 
GGTTTCATGT CCCAGGAAGA GAGGAAAAAG TTTGAGAGCC TGAAATCCGA CTTCAACAAG 
TACTGGGTCC CCTGCGTCTG GTTCACCAAC CTGGCGGCCC AGGCCCGGAG GGACGGGCGA 
ATACGTGACG ATATCGCTCT CTGTCTACTT TTGGAAGAGC TGAACAAGTA CCGAGCCAAG 
TGCAGCATGC TATTCCACTA TGACTGGATC AGCATCCCCC TCGTCTACAC CCAAGTGGTG 
ACCATAGCCG TCTACTCTTT CTTTGCCCTC TCCCTGGTTG GCCGCCAGTT TGTGGAGCCA 
GAGGCAGGGG CTGCCAAACC TCAGAAGCTT CTGAAGCCAG GCCAGGAGCC AGCCCCAGCC 
CTGGGAGACC CGGACATGTA CGTGCCTCTC ACCACTCTGC TGCAGTTCTT CTTCTATGCT 
GGCTGGCTCA AGGTGGCTGA ACAGATCATC AACCCATTTG GTGAGGATGA TGACGACTTT 
GAGACAAATC AGCTCATAGA CCGCAACTTG CAGGTGTCCC TGCTATCCGT GGACGAAATG 
TACCAGAACC TTCCCCCCGC TGAGAAGGAC CAGTACTGGG ATGAGGACCA GCCGCAGCCA 
CCCTACACTG TGGCCACGGC GGCCGAGTCT CTGCGGCCCT CATTCCTGGG CTCCACCTTC 
AACCTGCGCA TGAGCGACGA CCCTGAGCAG AGCCTGCAGG TGGAGGCGTC CCCCGGATCT 
GGTCGGCCCG CGCCCGCCGC GCAGACCCCG TTGCTCGGCC GCTTCCTGGG CGTAGGGGCG 
CCCTCCCCGG CCATCAGCCT CCGGAACTTC GGCCGCGTGC GAGGCACCCC CCGCCCCCCG 
CATCTGCTGC GCTTCCGGGC GGAGGAGGGC GGCGACCCCG AGGCCGCAGC CCGCATCGAG 
GAGGAATCGG CGGAGTCCGG GGACGAGGCC CTGGAGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST4 ( XM_017001023.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856512.1 Homo sapiens bestrophin 4 (BEST4), transcript variant X2, mRNA.

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CloneID	OHu06993
Clone ID Related Accession (Same CDS sequence)	XM_017001022.1 , NM_153274.2 , NM_153274.3 , XM_024446367.1 , XM_017001023.1
Accession Version	XM_017001023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bestrophin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGTTT CATACACTCT CAAAGTGGCG GAGGCCCGCT TCGGAGGTTT CTCTGGCCTG 
CTTCTCCGCT GGAGGGGAAG CATCTACAAG CTCCTCTACA AGGAATTCCT CCTCTTTGGG 
GCCTTGTACG CTGTGCTTAG CATCACCTAC CGGCTGCTGC TGACCCAGGA GCAGAGGTAC 
GTGTATGCTC AGGTGGCCCG GTACTGCAAC CGCTCAGCAG ACCTCATTCC CTTGTCCTTT 
GTATTGGGTT TCTATGTGAC TCTCGTGGTG AACCGCTGGT GGTCCCAGTA CACAAGCATC 
CCGCTGCCAG ACCAGCTGAT GTGCGTCATC TCGGCTAGCG TGCACGGCGT GGACCAGCGG 
GGCCGCCTGC TGCGCCGCAC CCTCATCCGC TACGCGAACC TGGCGTCCGT GCTGGTGCTG 
CGCTCGGTCA GCACCCGCGT GCTTAAGCGC TTCCCCACCA TGGAGCACGT GGTGGACGCA 
GGTTTCATGT CCCAGGAAGA GAGGAAAAAG TTTGAGAGCC TGAAATCCGA CTTCAACAAG 
TACTGGGTCC CCTGCGTCTG GTTCACCAAC CTGGCGGCCC AGGCCCGGAG GGACGGGCGA 
ATACGTGACG ATATCGCTCT CTGTCTACTT TTGGAAGAGC TGAACAAGTA CCGAGCCAAG 
TGCAGCATGC TATTCCACTA TGACTGGATC AGCATCCCCC TCGTCTACAC CCAAGTGGTG 
ACCATAGCCG TCTACTCTTT CTTTGCCCTC TCCCTGGTTG GCCGCCAGTT TGTGGAGCCA 
GAGGCAGGGG CTGCCAAACC TCAGAAGCTT CTGAAGCCAG GCCAGGAGCC AGCCCCAGCC 
CTGGGAGACC CGGACATGTA CGTGCCTCTC ACCACTCTGC TGCAGTTCTT CTTCTATGCT 
GGCTGGCTCA AGGTGGCTGA ACAGATCATC AACCCATTTG GTGAGGATGA TGACGACTTT 
GAGACAAATC AGCTCATAGA CCGCAACTTG CAGGTGTCCC TGCTATCCGT GGACGAAATG 
TACCAGAACC TTCCCCCCGC TGAGAAGGAC CAGTACTGGG ATGAGGACCA GCCGCAGCCA 
CCCTACACTG TGGCCACGGC GGCCGAGTCT CTGCGGCCCT CATTCCTGGG CTCCACCTTC 
AACCTGCGCA TGAGCGACGA CCCTGAGCAG AGCCTGCAGG TGGAGGCGTC CCCCGGATCT 
GGTCGGCCCG CGCCCGCCGC GCAGACCCCG TTGCTCGGCC GCTTCCTGGG CGTAGGGGCG 
CCCTCCCCGG CCATCAGCCT CCGGAACTTC GGCCGCGTGC GAGGCACCCC CCGCCCCCCG 
CATCTGCTGC GCTTCCGGGC GGAGGAGGGC GGCGACCCCG AGGCCGCAGC CCGCATCGAG 
GAGGAATCGG CGGAGTCCGG GGACGAGGCC CTGGAGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856512.1
CDS	1401..2822
Translation	MTVSYTLKVAEARFGGFSGLLLRWRGSIYKLLYKEFLLFGALYAVLSITYRLLLTQEQRYVYAQVARYCNRSADLIPLSFVLGFYVTLVVNRWWSQYTSIPLPDQLMCVISASVHGVDQRGRLLRRTLIRYANLASVLVLRSVSTRVLKRFPTMEHVVDAGFMSQEERKKFESLKSDFNKYWVPCVWFTNLAAQARRDGRIRDDIALCLLLEELNKYRAKCSMLFHYDWISIPLVYTQVVTIAVYSFFALSLVGRQFVEPEAGAAKPQKLLKPGQEPAPALGDPDMYVPLTTLLQFFFYAGWLKVAEQIINPFGEDDDDFETNQLIDRNLQVSLLSVDEMYQNLPPAEKDQYWDEDQPQPPYTVATAAESLRPSFLGSTFNLRMSDDPEQSLQVEASPGSGRPAPAAQTPLLGRFLGVGAPSPAISLRNFGRVRGTPRPPHLLRFRAEEGGDPEAAARIEEESAESGDEALEP

Target ORF information:

RefSeq Version	XM_017001023.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 4 (BEST4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001023.1

ORF Insert Sequence:

ATGACGGTTT CATACACTCT CAAAGTGGCG GAGGCCCGCT TCGGAGGTTT CTCTGGCCTG 
CTTCTCCGCT GGAGGGGAAG CATCTACAAG CTCCTCTACA AGGAATTCCT CCTCTTTGGG 
GCCTTGTACG CTGTGCTTAG CATCACCTAC CGGCTGCTGC TGACCCAGGA GCAGAGGTAC 
GTGTATGCTC AGGTGGCCCG GTACTGCAAC CGCTCAGCAG ACCTCATTCC CTTGTCCTTT 
GTATTGGGTT TCTATGTGAC TCTCGTGGTG AACCGCTGGT GGTCCCAGTA CACAAGCATC 
CCGCTGCCAG ACCAGCTGAT GTGCGTCATC TCGGCTAGCG TGCACGGCGT GGACCAGCGG 
GGCCGCCTGC TGCGCCGCAC CCTCATCCGC TACGCGAACC TGGCGTCCGT GCTGGTGCTG 
CGCTCGGTCA GCACCCGCGT GCTTAAGCGC TTCCCCACCA TGGAGCACGT GGTGGACGCA 
GGTTTCATGT CCCAGGAAGA GAGGAAAAAG TTTGAGAGCC TGAAATCCGA CTTCAACAAG 
TACTGGGTCC CCTGCGTCTG GTTCACCAAC CTGGCGGCCC AGGCCCGGAG GGACGGGCGA 
ATACGTGACG ATATCGCTCT CTGTCTACTT TTGGAAGAGC TGAACAAGTA CCGAGCCAAG 
TGCAGCATGC TATTCCACTA TGACTGGATC AGCATCCCCC TCGTCTACAC CCAAGTGGTG 
ACCATAGCCG TCTACTCTTT CTTTGCCCTC TCCCTGGTTG GCCGCCAGTT TGTGGAGCCA 
GAGGCAGGGG CTGCCAAACC TCAGAAGCTT CTGAAGCCAG GCCAGGAGCC AGCCCCAGCC 
CTGGGAGACC CGGACATGTA CGTGCCTCTC ACCACTCTGC TGCAGTTCTT CTTCTATGCT 
GGCTGGCTCA AGGTGGCTGA ACAGATCATC AACCCATTTG GTGAGGATGA TGACGACTTT 
GAGACAAATC AGCTCATAGA CCGCAACTTG CAGGTGTCCC TGCTATCCGT GGACGAAATG 
TACCAGAACC TTCCCCCCGC TGAGAAGGAC CAGTACTGGG ATGAGGACCA GCCGCAGCCA 
CCCTACACTG TGGCCACGGC GGCCGAGTCT CTGCGGCCCT CATTCCTGGG CTCCACCTTC 
AACCTGCGCA TGAGCGACGA CCCTGAGCAG AGCCTGCAGG TGGAGGCGTC CCCCGGATCT 
GGTCGGCCCG CGCCCGCCGC GCAGACCCCG TTGCTCGGCC GCTTCCTGGG CGTAGGGGCG 
CCCTCCCCGG CCATCAGCCT CCGGAACTTC GGCCGCGTGC GAGGCACCCC CCGCCCCCCG 
CATCTGCTGC GCTTCCGGGC GGAGGAGGGC GGCGACCCCG AGGCCGCAGC CCGCATCGAG 
GAGGAATCGG CGGAGTCCGG GGACGAGGCC CTGGAGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST4 ( XM_017001020.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856509.1 Homo sapiens bestrophin 4 (BEST4), transcript variant X1, mRNA.

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CloneID	OHu87249
Clone ID Related Accession (Same CDS sequence)	XM_017001020.1 , XM_017001021.1
Accession Version	XM_017001020.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	bestrophin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGTTT CATACACTCT CAAAGTGGCG GAGGCCCGCT TCGGAGGTTT CTCTGGCCTG 
CTTCTCCGCT GGAGGGGAAG CATCTACAAG CTCCTCTACA AGGAATTCCT CCTCTTTGGG 
GCCTTGTACG CTGTGCTTAG CATCACCTAC CGGCTGCTGC TGACCCAGGA GCAGAGGTAC 
GTGTATGCTC AGGTGGCCCG GTACTGCAAC CGCTCAGCAG ACCTCATTCC CTTGTCCTTT 
GTATTGGGTT TCTATGTGAC TCTCGTGGTG AACCGCTGGT GGTCCCAGTA CACAAGCATC 
CCGCTGCCAG ACCAGCTGAT GTGCGTCATC TCGGCTAGCG TGCACGGCGT GGACCAGCGG 
GGCCGCCTGC TGCGCCGCAC CCTCATCCGC TACGCGAACC TGGCGTCCGT GCTGGTGCTG 
CGCTCGGTCA GCACCCGCGT GCTTAAGCGC TTCCCCACCA TGGAGCACGT GGTGGACGCA 
GGTTTCATGT CCCAGGAAGA GAGGAAAAAG TTTGAGAGCC TGAAATCCGA CTTCAACAAG 
TACTGGGTCC CCTGCGTCTG GTTCACCAAC CTGGCGGCCC AGGCCCGGAG GGACGGGCGA 
ATACGTGACG ATATCGCTCT CTGTCTACTT TTGGAAGAGC TGAACAAGTA CCGAGCCAAG 
TGCAGCATGC TATTCCACTA TGACTGGATC AGCATCCCCC TCGTCTACAC CCAAGTGGTG 
ACCATAGCCG TCTACTCTTT CTTTGCCCTC TCCCTGGTTG GCCGCCAGTT TGTGGAGCCA 
GAGGCAGGGG CTGCCAAACC TCAGAAGCTT CTGAAGCCAG GCCAGGAGCC AGCCCCAGCC 
CTGGGAGACC CGGACATGTA CGTGCCTCTC ACCACTCTGC TGCAGTTCTT CTTCTATGCT 
GGCTGGCTCA AGGTGGCTGA ACAGATCATC AACCCATTTG GTGAGGATGA TGACGACTTT 
GAGACAAATC AGCTCATAGA CCGCAACTTG CAGGTGTCCC TGCTATCCGT GGACGAAATG 
TACCAGAACC TTCCCCCCGC TGAGAAGGAC CAGTACTGGG ATGAGGACCA GCCGCAGCCA 
CCCTACACTG TGGCCACGGC GGCCGAGTCT CTGCGGCCCT CATTCCTGGG CTCCACCTTC 
AACCTGCGCT TTTACAAGCC TTGTTCCATG GTGAGAACGG TCATCTGTGA AGGTGGTAGA 
AATGGAGAAT GGATGGATTT CAAGACTTTA GAGAAAGAAC CCACTGGTTT TATGGACAGA 
TCTTGGCTCA CTGCAACCTC CACCTCCCCA GGTTCAAGAG ATTCTCATGC CTCAGCCTCT 
CAAGTAGCTG GGATTACAGG CACATGCCAC CACACCCTGC TAATTTTTTG TATGTTTAGT 
AGAGATGGGG TTTCACCATG TTGGCCAGGC TGGTTTTTGA ACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856509.1
CDS	1298..2725
Translation	MTVSYTLKVAEARFGGFSGLLLRWRGSIYKLLYKEFLLFGALYAVLSITYRLLLTQEQRYVYAQVARYCNRSADLIPLSFVLGFYVTLVVNRWWSQYTSIPLPDQLMCVISASVHGVDQRGRLLRRTLIRYANLASVLVLRSVSTRVLKRFPTMEHVVDAGFMSQEERKKFESLKSDFNKYWVPCVWFTNLAAQARRDGRIRDDIALCLLLEELNKYRAKCSMLFHYDWISIPLVYTQVVTIAVYSFFALSLVGRQFVEPEAGAAKPQKLLKPGQEPAPALGDPDMYVPLTTLLQFFFYAGWLKVAEQIINPFGEDDDDFETNQLIDRNLQVSLLSVDEMYQNLPPAEKDQYWDEDQPQPPYTVATAAESLRPSFLGSTFNLRFYKPCSMVRTVICEGGRNGEWMDFKTLEKEPTGFMDRSWLTATSTSPGSRDSHASASQVAGITGTCHHTLLIFCMFSRDGVSPCWPGWFLNS

Target ORF information:

RefSeq Version	XM_017001020.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 4 (BEST4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001020.1

ORF Insert Sequence:

ATGACGGTTT CATACACTCT CAAAGTGGCG GAGGCCCGCT TCGGAGGTTT CTCTGGCCTG 
CTTCTCCGCT GGAGGGGAAG CATCTACAAG CTCCTCTACA AGGAATTCCT CCTCTTTGGG 
GCCTTGTACG CTGTGCTTAG CATCACCTAC CGGCTGCTGC TGACCCAGGA GCAGAGGTAC 
GTGTATGCTC AGGTGGCCCG GTACTGCAAC CGCTCAGCAG ACCTCATTCC CTTGTCCTTT 
GTATTGGGTT TCTATGTGAC TCTCGTGGTG AACCGCTGGT GGTCCCAGTA CACAAGCATC 
CCGCTGCCAG ACCAGCTGAT GTGCGTCATC TCGGCTAGCG TGCACGGCGT GGACCAGCGG 
GGCCGCCTGC TGCGCCGCAC CCTCATCCGC TACGCGAACC TGGCGTCCGT GCTGGTGCTG 
CGCTCGGTCA GCACCCGCGT GCTTAAGCGC TTCCCCACCA TGGAGCACGT GGTGGACGCA 
GGTTTCATGT CCCAGGAAGA GAGGAAAAAG TTTGAGAGCC TGAAATCCGA CTTCAACAAG 
TACTGGGTCC CCTGCGTCTG GTTCACCAAC CTGGCGGCCC AGGCCCGGAG GGACGGGCGA 
ATACGTGACG ATATCGCTCT CTGTCTACTT TTGGAAGAGC TGAACAAGTA CCGAGCCAAG 
TGCAGCATGC TATTCCACTA TGACTGGATC AGCATCCCCC TCGTCTACAC CCAAGTGGTG 
ACCATAGCCG TCTACTCTTT CTTTGCCCTC TCCCTGGTTG GCCGCCAGTT TGTGGAGCCA 
GAGGCAGGGG CTGCCAAACC TCAGAAGCTT CTGAAGCCAG GCCAGGAGCC AGCCCCAGCC 
CTGGGAGACC CGGACATGTA CGTGCCTCTC ACCACTCTGC TGCAGTTCTT CTTCTATGCT 
GGCTGGCTCA AGGTGGCTGA ACAGATCATC AACCCATTTG GTGAGGATGA TGACGACTTT 
GAGACAAATC AGCTCATAGA CCGCAACTTG CAGGTGTCCC TGCTATCCGT GGACGAAATG 
TACCAGAACC TTCCCCCCGC TGAGAAGGAC CAGTACTGGG ATGAGGACCA GCCGCAGCCA 
CCCTACACTG TGGCCACGGC GGCCGAGTCT CTGCGGCCCT CATTCCTGGG CTCCACCTTC 
AACCTGCGCT TTTACAAGCC TTGTTCCATG GTGAGAACGG TCATCTGTGA AGGTGGTAGA 
AATGGAGAAT GGATGGATTT CAAGACTTTA GAGAAAGAAC CCACTGGTTT TATGGACAGA 
TCTTGGCTCA CTGCAACCTC CACCTCCCCA GGTTCAAGAG ATTCTCATGC CTCAGCCTCT 
CAAGTAGCTG GGATTACAGG CACATGCCAC CACACCCTGC TAATTTTTTG TATGTTTAGT 
AGAGATGGGG TTTCACCATG TTGGCCAGGC TGGTTTTTGA ACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST4 ( XM_017001021.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856510.1 Homo sapiens bestrophin 4 (BEST4), transcript variant X2, mRNA.

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CloneID	OHu87249
Clone ID Related Accession (Same CDS sequence)	XM_017001020.1 , XM_017001021.1
Accession Version	XM_017001021.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	bestrophin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGTTT CATACACTCT CAAAGTGGCG GAGGCCCGCT TCGGAGGTTT CTCTGGCCTG 
CTTCTCCGCT GGAGGGGAAG CATCTACAAG CTCCTCTACA AGGAATTCCT CCTCTTTGGG 
GCCTTGTACG CTGTGCTTAG CATCACCTAC CGGCTGCTGC TGACCCAGGA GCAGAGGTAC 
GTGTATGCTC AGGTGGCCCG GTACTGCAAC CGCTCAGCAG ACCTCATTCC CTTGTCCTTT 
GTATTGGGTT TCTATGTGAC TCTCGTGGTG AACCGCTGGT GGTCCCAGTA CACAAGCATC 
CCGCTGCCAG ACCAGCTGAT GTGCGTCATC TCGGCTAGCG TGCACGGCGT GGACCAGCGG 
GGCCGCCTGC TGCGCCGCAC CCTCATCCGC TACGCGAACC TGGCGTCCGT GCTGGTGCTG 
CGCTCGGTCA GCACCCGCGT GCTTAAGCGC TTCCCCACCA TGGAGCACGT GGTGGACGCA 
GGTTTCATGT CCCAGGAAGA GAGGAAAAAG TTTGAGAGCC TGAAATCCGA CTTCAACAAG 
TACTGGGTCC CCTGCGTCTG GTTCACCAAC CTGGCGGCCC AGGCCCGGAG GGACGGGCGA 
ATACGTGACG ATATCGCTCT CTGTCTACTT TTGGAAGAGC TGAACAAGTA CCGAGCCAAG 
TGCAGCATGC TATTCCACTA TGACTGGATC AGCATCCCCC TCGTCTACAC CCAAGTGGTG 
ACCATAGCCG TCTACTCTTT CTTTGCCCTC TCCCTGGTTG GCCGCCAGTT TGTGGAGCCA 
GAGGCAGGGG CTGCCAAACC TCAGAAGCTT CTGAAGCCAG GCCAGGAGCC AGCCCCAGCC 
CTGGGAGACC CGGACATGTA CGTGCCTCTC ACCACTCTGC TGCAGTTCTT CTTCTATGCT 
GGCTGGCTCA AGGTGGCTGA ACAGATCATC AACCCATTTG GTGAGGATGA TGACGACTTT 
GAGACAAATC AGCTCATAGA CCGCAACTTG CAGGTGTCCC TGCTATCCGT GGACGAAATG 
TACCAGAACC TTCCCCCCGC TGAGAAGGAC CAGTACTGGG ATGAGGACCA GCCGCAGCCA 
CCCTACACTG TGGCCACGGC GGCCGAGTCT CTGCGGCCCT CATTCCTGGG CTCCACCTTC 
AACCTGCGCT TTTACAAGCC TTGTTCCATG GTGAGAACGG TCATCTGTGA AGGTGGTAGA 
AATGGAGAAT GGATGGATTT CAAGACTTTA GAGAAAGAAC CCACTGGTTT TATGGACAGA 
TCTTGGCTCA CTGCAACCTC CACCTCCCCA GGTTCAAGAG ATTCTCATGC CTCAGCCTCT 
CAAGTAGCTG GGATTACAGG CACATGCCAC CACACCCTGC TAATTTTTTG TATGTTTAGT 
AGAGATGGGG TTTCACCATG TTGGCCAGGC TGGTTTTTGA ACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856510.1
CDS	277..1704
Translation	MTVSYTLKVAEARFGGFSGLLLRWRGSIYKLLYKEFLLFGALYAVLSITYRLLLTQEQRYVYAQVARYCNRSADLIPLSFVLGFYVTLVVNRWWSQYTSIPLPDQLMCVISASVHGVDQRGRLLRRTLIRYANLASVLVLRSVSTRVLKRFPTMEHVVDAGFMSQEERKKFESLKSDFNKYWVPCVWFTNLAAQARRDGRIRDDIALCLLLEELNKYRAKCSMLFHYDWISIPLVYTQVVTIAVYSFFALSLVGRQFVEPEAGAAKPQKLLKPGQEPAPALGDPDMYVPLTTLLQFFFYAGWLKVAEQIINPFGEDDDDFETNQLIDRNLQVSLLSVDEMYQNLPPAEKDQYWDEDQPQPPYTVATAAESLRPSFLGSTFNLRFYKPCSMVRTVICEGGRNGEWMDFKTLEKEPTGFMDRSWLTATSTSPGSRDSHASASQVAGITGTCHHTLLIFCMFSRDGVSPCWPGWFLNS

Target ORF information:

RefSeq Version	XM_017001021.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 4 (BEST4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001021.1

ORF Insert Sequence:

ATGACGGTTT CATACACTCT CAAAGTGGCG GAGGCCCGCT TCGGAGGTTT CTCTGGCCTG 
CTTCTCCGCT GGAGGGGAAG CATCTACAAG CTCCTCTACA AGGAATTCCT CCTCTTTGGG 
GCCTTGTACG CTGTGCTTAG CATCACCTAC CGGCTGCTGC TGACCCAGGA GCAGAGGTAC 
GTGTATGCTC AGGTGGCCCG GTACTGCAAC CGCTCAGCAG ACCTCATTCC CTTGTCCTTT 
GTATTGGGTT TCTATGTGAC TCTCGTGGTG AACCGCTGGT GGTCCCAGTA CACAAGCATC 
CCGCTGCCAG ACCAGCTGAT GTGCGTCATC TCGGCTAGCG TGCACGGCGT GGACCAGCGG 
GGCCGCCTGC TGCGCCGCAC CCTCATCCGC TACGCGAACC TGGCGTCCGT GCTGGTGCTG 
CGCTCGGTCA GCACCCGCGT GCTTAAGCGC TTCCCCACCA TGGAGCACGT GGTGGACGCA 
GGTTTCATGT CCCAGGAAGA GAGGAAAAAG TTTGAGAGCC TGAAATCCGA CTTCAACAAG 
TACTGGGTCC CCTGCGTCTG GTTCACCAAC CTGGCGGCCC AGGCCCGGAG GGACGGGCGA 
ATACGTGACG ATATCGCTCT CTGTCTACTT TTGGAAGAGC TGAACAAGTA CCGAGCCAAG 
TGCAGCATGC TATTCCACTA TGACTGGATC AGCATCCCCC TCGTCTACAC CCAAGTGGTG 
ACCATAGCCG TCTACTCTTT CTTTGCCCTC TCCCTGGTTG GCCGCCAGTT TGTGGAGCCA 
GAGGCAGGGG CTGCCAAACC TCAGAAGCTT CTGAAGCCAG GCCAGGAGCC AGCCCCAGCC 
CTGGGAGACC CGGACATGTA CGTGCCTCTC ACCACTCTGC TGCAGTTCTT CTTCTATGCT 
GGCTGGCTCA AGGTGGCTGA ACAGATCATC AACCCATTTG GTGAGGATGA TGACGACTTT 
GAGACAAATC AGCTCATAGA CCGCAACTTG CAGGTGTCCC TGCTATCCGT GGACGAAATG 
TACCAGAACC TTCCCCCCGC TGAGAAGGAC CAGTACTGGG ATGAGGACCA GCCGCAGCCA 
CCCTACACTG TGGCCACGGC GGCCGAGTCT CTGCGGCCCT CATTCCTGGG CTCCACCTTC 
AACCTGCGCT TTTACAAGCC TTGTTCCATG GTGAGAACGG TCATCTGTGA AGGTGGTAGA 
AATGGAGAAT GGATGGATTT CAAGACTTTA GAGAAAGAAC CCACTGGTTT TATGGACAGA 
TCTTGGCTCA CTGCAACCTC CACCTCCCCA GGTTCAAGAG ATTCTCATGC CTCAGCCTCT 
CAAGTAGCTG GGATTACAGG CACATGCCAC CACACCCTGC TAATTTTTTG TATGTTTAGT 
AGAGATGGGG TTTCACCATG TTGGCCAGGC TGGTTTTTGA ACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lineage-specific expression of bestrophin-2 and bestrophin-4 in human intestinal epithelial cells.
PloS one8(11)e79693(2013)
Ito G,Okamoto R,Murano T,Shimizu H,Fujii S,Nakata T,Mizutani T,Yui S,Akiyama-Morio J,Nemoto Y,Okada E,Araki A,Ohtsuka K,Tsuchiya K,Nakamura T,Watanabe M

Bestrophin expression and function in the human pancreatic duct cell line, CFPAC-1.
The Journal of physiology587(Pt 10)2211-24(2009 May)
Marsey LL,Winpenny JP

Ca2+-activated Cl- current from human bestrophin-4 in excised membrane patches.
The Journal of general physiology127(6)749-54(2006 Jun)
Tsunenari T,Nathans J,Yau KW

Structure-function analysis of the bestrophin family of anion channels.
The Journal of biological chemistry278(42)41114-25(2003 Oct)
Tsunenari T,Sun H,Williams J,Cahill H,Smallwood P,Yau KW,Nathans J

Three novel human VMD2-like genes are members of the evolutionary highly conserved RFP-TM family.
European journal of human genetics : EJHG10(4)281-4(2002 Apr)
St?hr H,Marquardt A,Nanda I,Schmid M,Weber BH

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BEST4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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