COTL1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	COTL1
Entrez Gene ID	23406
Full Name	coactosin like F-actin binding protein 1
Synonyms	CLP
General protein information	Preferred Names coactosin like F-actin binding protein 1 Names coactosin-like protein coactosin-like 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q24.1
Summary	This gene encodes one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis. Although this gene has been reported to map to chromosome 17 in the Smith-Magenis syndrome region, the best alignments for this gene are to chromosome 16. The Smith-Magenis syndrome region is the site of two related pseudogenes. [provided by RefSeq, Jul 2008].
Disorder MIM:	606748

mRNA and Protein(s)

mRNA	Protein	Name
NM_021149.4	NP_066972.1	coactosin-like protein
NM_021149.5	NP_066972.1	coactosin-like protein
NM_021149.3	NP_066972.1	coactosin-like protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	COTL1	NP_066972.1
Bos taurus (cattle)	COTL1	NP_001040058.1
Gallus gallus (chicken)	COTL1	NP_001159797.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000889	XP_316866.4
Danio rerio (zebrafish)	cotl1	NP_956306.1
Drosophila melanogaster (fruit fly)	CG6891	NP_573323.1
Xenopus tropicalis (tropical clawed frog)	cotl1	NP_001016541.1
	COTL1	XP_001112723.1
Canis lupus familiaris (dog)	COTL1	XP_546800.3
Rattus norvegicus (Norway rat)	Cotl1	NP_001101922.1
Mus musculus (house mouse)	Cotl1	NP_082347.1

Gene Ontology
Bibliography

Related articles in PubMed

Coactosin-like protein CLP/Cotl1 suppresses breast cancer growth through activation of IL-24/PERP and inhibition of non-canonical TGF? signaling.
Xia L, Xiao X, Liu WL, Song Y, Liu TJJ, Li YJ, Zacksenhaus E, Hao XJ, Ben-David Y
Oncogene37(3)323-331(2018 Jan)

Genetic and epigenetic silencing of mircoRNA-506-3p enhances COTL1 oncogene expression to foster non-small lung cancer progression.
Guo S, Yang P, Jiang X, Li X, Wang Y, Zhang X, Sun B, Zhang Y, Jia Y
Oncotarget8(1)644-657(2017 Jan)

Roles of coactosin-like protein (CLP) and 5-lipoxygenase-activating protein (FLAP) in cellular leukotriene biosynthesis.
Basavarajappa D, Wan M, Lukic A, Steinhilber D, Samuelsson B, R?dmark O
Proceedings of the National Academy of Sciences of the United States of America111(31)11371-6(2014 Aug)

Coactosin-like 1 antagonizes cofilin to promote lamellipodial protrusion at the immune synapse.
Kim J, Shapiro MJ, Bamidele AO, Gurel P, Thapa P, Higgs HN, Hedin KE, Shapiro VS, Billadeau DD
PloS one9(1)e85090(2014)

Differential expression of PAI-RBP1, C1orf142, and COTL1 in non-small cell lung cancer cell lines with different tumor metastatic potential.
Sun W, Guo C, Meng X, Yu Y, Jin Y, Tong D, Geng J, Huang Q, Qi J, Liu A, Guan R, Xu L, Sun D, Ji W, Liu P, Liu F, Sun H, Ji G, Fu S, Bai J
Journal of investigative medicine : the official publication of the American Federation for Clinical Research60(4)689-94(2012 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The roles of CLP and FLAP in in cellular leukotriene biosynthesis, were studied.
Title: Roles of coactosin-like protein (CLP) and 5-lipoxygenase-activating protein (FLAP) in cellular leukotriene biosynthesis.

COTL1 regulates lamellipodia dynamics in part by protecting F-actin from cofilin-mediated disassembly.
Title: Coactosin-like 1 antagonizes cofilin to promote lamellipodial protrusion at the immune synapse.

PAI-RBP1 and C1orf142 expression levels are higher in 95D (high metastatic potential) than in 95C (low metastatic potential) non-small lung cancer cells, whereas COTL1 expression level is lower in 95D when compared to 95C cells.
Title: Differential expression of PAI-RBP1, C1orf142, and COTL1 in non-small cell lung cancer cell lines with different tumor metastatic potential.

Up-regulated COTL-1 expression in small cell lung cancer was validated by Western blot analysis, immunohistochemistry, and qRT-qPCR.
Title: Proteomic analysis of human small cell lung cancer tissues: up-regulation of coactosin-like protein-1.

Results of the present study support a role for CLP as a chaperoning scaffold factor, influencing both the stability and the activity of 5-LO.
Title: Coactosin-like protein functions as a stabilizing chaperone for 5-lipoxygenase: role of tryptophan 102.

The following COTL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COTL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27498	NM_021149.5 Latest version!	Homo sapiens coactosin like F-actin binding protein 1 (COTL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu27498	NM_021149.4	Homo sapiens coactosin like F-actin binding protein 1 (COTL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu27498	NM_021149.3	Homo sapiens coactosin like F-actin binding protein 1 (COTL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COTL1 ( NM_021149.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_066972.1 Homo sapiens coactosin like F-actin binding protein 1 (COTL1), mRNA.

Price & Availability↑

CloneID	OHu27498
Clone ID Related Accession (Same CDS sequence)	NM_021149.4 , NM_021149.5 , NM_021149.3
Accession Version	NM_021149.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	coactosin-like protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA968872.1 and BC010884.1. On Nov 22, 2018 this sequence version replaced NM_021149.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC010039.2, BC042970.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCACCA AGATCGACAA AGAGGCTTGC CGGGCGGCGT ACAACCTGGT GCGCGACGAC 
GGCTCGGCCG TCATCTGGGT GACTTTTAAA TATGACGGCT CCACCATCGT CCCCGGCGAG 
CAGGGAGCGG AGTACCAGCA CTTCATCCAG CAGTGCACAG ATGACGTCCG GTTGTTTGCC 
TTCGTGCGCT TCACCACCGG GGATGCCATG AGCAAGAGGT CCAAGTTTGC CCTCATCACG 
TGGATCGGTG AGAACGTCAG CGGGCTGCAG CGCGCCAAAA CCGGGACGGA CAAGACCCTG 
GTGAAGGAGG TCGTACAGAA TTTCGCTAAG GAGTTTGTGA TCAGTGATCG GAAGGAGCTG 
GAGGAAGATT TCATCAAGAG CGAGCTGAAG AAGGCGGGGG GAGCCAATTA CGACGCCCAG 
ACGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066972.1
CDS	165..593
Translation	MATKIDKEACRAAYNLVRDDGSAVIWVTFKYDGSTIVPGEQGAEYQHFIQQCTDDVRLFAFVRFTTGDAMSKRSKFALITWIGENVSGLQRAKTGTDKTLVKEVVQNFAKEFVISDRKELEEDFIKSELKKAGGANYDAQTE

Target ORF information:

RefSeq Version	NM_021149.4
Organism	Homo sapiens(human)
Definition	Homo sapiens coactosin like F-actin binding protein 1 (COTL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021149.4

ORF Insert Sequence:

ATGGCCACCA AGATCGACAA AGAGGCTTGC CGGGCGGCGT ACAACCTGGT GCGCGACGAC 
GGCTCGGCCG TCATCTGGGT GACTTTTAAA TATGACGGCT CCACCATCGT CCCCGGCGAG 
CAGGGAGCGG AGTACCAGCA CTTCATCCAG CAGTGCACAG ATGACGTCCG GTTGTTTGCC 
TTCGTGCGCT TCACCACCGG GGATGCCATG AGCAAGAGGT CCAAGTTTGC CCTCATCACG 
TGGATCGGTG AGAACGTCAG CGGGCTGCAG CGCGCCAAAA CCGGGACGGA CAAGACCCTG 
GTGAAGGAGG TCGTACAGAA TTTCGCTAAG GAGTTTGTGA TCAGTGATCG GAAGGAGCTG 
GAGGAAGATT TCATCAAGAG CGAGCTGAAG AAGGCGGGGG GAGCCAATTA CGACGCCCAG 
ACGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COTL1 ( NM_021149.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_066972.1 Homo sapiens coactosin like F-actin binding protein 1 (COTL1), mRNA.

Price & Availability↑

CloneID	OHu27498
Clone ID Related Accession (Same CDS sequence)	NM_021149.4 , NM_021149.5 , NM_021149.3
Accession Version	NM_021149.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-14
Organism	Homo sapiens(human)
Product	coactosin-like protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092145.2 and AC022165.9. On Aug 31, 2019 this sequence version replaced NM_021149.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC010884.1, L54057.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000262428.5/ ENSP00000262428.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCACCA AGATCGACAA AGAGGCTTGC CGGGCGGCGT ACAACCTGGT GCGCGACGAC 
GGCTCGGCCG TCATCTGGGT GACTTTTAAA TATGACGGCT CCACCATCGT CCCCGGCGAG 
CAGGGAGCGG AGTACCAGCA CTTCATCCAG CAGTGCACAG ATGACGTCCG GTTGTTTGCC 
TTCGTGCGCT TCACCACCGG GGATGCCATG AGCAAGAGGT CCAAGTTTGC CCTCATCACG 
TGGATCGGTG AGAACGTCAG CGGGCTGCAG CGCGCCAAAA CCGGGACGGA CAAGACCCTG 
GTGAAGGAGG TCGTACAGAA TTTCGCTAAG GAGTTTGTGA TCAGTGATCG GAAGGAGCTG 
GAGGAAGATT TCATCAAGAG CGAGCTGAAG AAGGCGGGGG GAGCCAATTA CGACGCCCAG 
ACGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066972.1
CDS	165..593
Translation	MATKIDKEACRAAYNLVRDDGSAVIWVTFKYDGSTIVPGEQGAEYQHFIQQCTDDVRLFAFVRFTTGDAMSKRSKFALITWIGENVSGLQRAKTGTDKTLVKEVVQNFAKEFVISDRKELEEDFIKSELKKAGGANYDAQTE

Target ORF information:

RefSeq Version	NM_021149.5
Organism	Homo sapiens(human)
Definition	Homo sapiens coactosin like F-actin binding protein 1 (COTL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021149.5

ORF Insert Sequence:

ATGGCCACCA AGATCGACAA AGAGGCTTGC CGGGCGGCGT ACAACCTGGT GCGCGACGAC 
GGCTCGGCCG TCATCTGGGT GACTTTTAAA TATGACGGCT CCACCATCGT CCCCGGCGAG 
CAGGGAGCGG AGTACCAGCA CTTCATCCAG CAGTGCACAG ATGACGTCCG GTTGTTTGCC 
TTCGTGCGCT TCACCACCGG GGATGCCATG AGCAAGAGGT CCAAGTTTGC CCTCATCACG 
TGGATCGGTG AGAACGTCAG CGGGCTGCAG CGCGCCAAAA CCGGGACGGA CAAGACCCTG 
GTGAAGGAGG TCGTACAGAA TTTCGCTAAG GAGTTTGTGA TCAGTGATCG GAAGGAGCTG 
GAGGAAGATT TCATCAAGAG CGAGCTGAAG AAGGCGGGGG GAGCCAATTA CGACGCCCAG 
ACGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COTL1 ( NM_021149.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_066972.1 Homo sapiens coactosin like F-actin binding protein 1 (COTL1), mRNA.

Price & Availability↑

CloneID	OHu27498
Clone ID Related Accession (Same CDS sequence)	NM_021149.4 , NM_021149.5 , NM_021149.3
Accession Version	NM_021149.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	coactosin-like protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX395081.2, DA968872.1 and BC010884.1. On Sep 7, 2013 this sequence version replaced NM_021149.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC053682.1, SRR1163658.235569.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACCA AGATCGACAA AGAGGCTTGC CGGGCGGCGT ACAACCTGGT GCGCGACGAC 
GGCTCGGCCG TCATCTGGGT GACTTTTAAA TATGACGGCT CCACCATCGT CCCCGGCGAG 
CAGGGAGCGG AGTACCAGCA CTTCATCCAG CAGTGCACAG ATGACGTCCG GTTGTTTGCC 
TTCGTGCGCT TCACCACCGG GGATGCCATG AGCAAGAGGT CCAAGTTTGC CCTCATCACG 
TGGATCGGTG AGAACGTCAG CGGGCTGCAG CGCGCCAAAA CCGGGACGGA CAAGACCCTG 
GTGAAGGAGG TCGTACAGAA TTTCGCTAAG GAGTTTGTGA TCAGTGATCG GAAGGAGCTG 
GAGGAAGATT TCATCAAGAG CGAGCTGAAG AAGGCGGGGG GAGCCAATTA CGACGCCCAG 
ACGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066972.1
CDS	183..611
Translation	MATKIDKEACRAAYNLVRDDGSAVIWVTFKYDGSTIVPGEQGAEYQHFIQQCTDDVRLFAFVRFTTGDAMSKRSKFALITWIGENVSGLQRAKTGTDKTLVKEVVQNFAKEFVISDRKELEEDFIKSELKKAGGANYDAQTE

Target ORF information:

RefSeq Version	NM_021149.3
Organism	Homo sapiens(human)
Definition	Homo sapiens coactosin like F-actin binding protein 1 (COTL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021149.3

ORF Insert Sequence:

ATGGCCACCA AGATCGACAA AGAGGCTTGC CGGGCGGCGT ACAACCTGGT GCGCGACGAC 
GGCTCGGCCG TCATCTGGGT GACTTTTAAA TATGACGGCT CCACCATCGT CCCCGGCGAG 
CAGGGAGCGG AGTACCAGCA CTTCATCCAG CAGTGCACAG ATGACGTCCG GTTGTTTGCC 
TTCGTGCGCT TCACCACCGG GGATGCCATG AGCAAGAGGT CCAAGTTTGC CCTCATCACG 
TGGATCGGTG AGAACGTCAG CGGGCTGCAG CGCGCCAAAA CCGGGACGGA CAAGACCCTG 
GTGAAGGAGG TCGTACAGAA TTTCGCTAAG GAGTTTGTGA TCAGTGATCG GAAGGAGCTG 
GAGGAAGATT TCATCAAGAG CGAGCTGAAG AAGGCGGGGG GAGCCAATTA CGACGCCCAG 
ACGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Coactosin-like protein CLP/Cotl1 suppresses breast cancer growth through activation of IL-24/PERP and inhibition of non-canonical TGF? signaling.
Oncogene37(3)323-331(2018 Jan)
Xia L,Xiao X,Liu WL,Song Y,Liu TJJ,Li YJ,Zacksenhaus E,Hao XJ,Ben-David Y

Genetic and epigenetic silencing of mircoRNA-506-3p enhances COTL1 oncogene expression to foster non-small lung cancer progression.
Oncotarget8(1)644-657(2017 Jan)
Guo S,Yang P,Jiang X,Li X,Wang Y,Zhang X,Sun B,Zhang Y,Jia Y

Roles of coactosin-like protein (CLP) and 5-lipoxygenase-activating protein (FLAP) in cellular leukotriene biosynthesis.
Proceedings of the National Academy of Sciences of the United States of America111(31)11371-6(2014 Aug)
Basavarajappa D,Wan M,Lukic A,Steinhilber D,Samuelsson B,R?dmark O

Coactosin-like 1 antagonizes cofilin to promote lamellipodial protrusion at the immune synapse.
PloS one9(1)e85090(2014)
Kim J,Shapiro MJ,Bamidele AO,Gurel P,Thapa P,Higgs HN,Hedin KE,Shapiro VS,Billadeau DD

Differential expression of PAI-RBP1, C1orf142, and COTL1 in non-small cell lung cancer cell lines with different tumor metastatic potential.
Journal of investigative medicine : the official publication of the American Federation for Clinical Research60(4)689-94(2012 Apr)
Sun W,Guo C,Meng X,Yu Y,Jin Y,Tong D,Geng J,Huang Q,Qi J,Liu A,Guan R,Xu L,Sun D,Ji W,Liu P,Liu F,Sun H,Ji G,Fu S,Bai J

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COTL1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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