ADGRF5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ADGRF5
Entrez Gene ID	221395
Full Name	adhesion G protein-coupled receptor F5
Synonyms	GPR116,KPG_001
General protein information	Preferred Names adhesion G protein-coupled receptor F5 Names adhesion G protein-coupled receptor F5 G-protein coupled receptor 116 Ig-Hepta homolog probable G-protein coupled receptor 116
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p12.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_005248895.3	XP_005248952.1	adhesion G protein-coupled receptor F5 isoform X3
NM_001098518.2	NP_001091988.1	adhesion G protein-coupled receptor F5 precursor
XM_011514354.2	XP_011512656.1	adhesion G protein-coupled receptor F5 isoform X7
XM_017010421.1	XP_016865910.1	adhesion G protein-coupled receptor F5 isoform X5
XM_005248892.3	XP_005248949.1	adhesion G protein-coupled receptor F5 isoform X1
XM_005248894.4	XP_005248951.1	adhesion G protein-coupled receptor F5 isoform X2
XM_017010419.2	XP_016865908.1	adhesion G protein-coupled receptor F5 isoform X4
NM_015234.4	NP_056049.4	adhesion G protein-coupled receptor F5 precursor
XM_017010424.1	XP_016865913.1	adhesion G protein-coupled receptor F5 isoform X9
NM_015234.5	NP_056049.4	adhesion G protein-coupled receptor F5 precursor
XM_005248893.3	XP_005248950.1	adhesion G protein-coupled receptor F5 isoform X1
XM_011514353.3	XP_011512655.1	adhesion G protein-coupled receptor F5 isoform X4
XM_017010424.2	XP_016865913.1	adhesion G protein-coupled receptor F5 isoform X9
XM_005248894.3	XP_005248951.1	adhesion G protein-coupled receptor F5 isoform X2
XM_011514353.2	XP_011512655.1	adhesion G protein-coupled receptor F5 isoform X4
XM_017010420.1	XP_016865909.1	adhesion G protein-coupled receptor F5 isoform X5
XM_017010418.2	XP_016865907.1	adhesion G protein-coupled receptor F5 isoform X3
NM_001098518.1	NP_001091988.1	adhesion G protein-coupled receptor F5 precursor
XM_005248892.2	XP_005248949.1	adhesion G protein-coupled receptor F5 isoform X1
XM_017010418.1	XP_016865907.1	adhesion G protein-coupled receptor F5 isoform X3
XM_017010419.1	XP_016865908.1	adhesion G protein-coupled receptor F5 isoform X4
XM_017010422.1	XP_016865911.1	adhesion G protein-coupled receptor F5 isoform X6
XM_005248895.4	XP_005248952.1	adhesion G protein-coupled receptor F5 isoform X3
XM_017010420.2	XP_016865909.1	adhesion G protein-coupled receptor F5 isoform X5
XM_017010421.2	XP_016865910.1	adhesion G protein-coupled receptor F5 isoform X5
XM_017010422.2	XP_016865911.1	adhesion G protein-coupled receptor F5 isoform X6
XM_017010423.1	XP_016865912.1	adhesion G protein-coupled receptor F5 isoform X8

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GPR116	XP_001140861.2
Gallus gallus (chicken)	GPR116	XP_004935990.1
Xenopus tropicalis (tropical clawed frog)	gpr116	NP_001107298.1
Mus musculus (house mouse)	Gpr116	NP_001074647.1
Homo sapiens (human)	GPR116	NP_001091988.1
	GPR116	XP_001103527.2
Canis lupus familiaris (dog)	GPR116	XP_005627499.1
Bos taurus (cattle)	GPR116	NP_001180172.1
Rattus norvegicus (Norway rat)	Gpr116	NP_620810.1

Gene Ontology
Bibliography

Related articles in PubMed

High expression of GPR116 indicates poor survival outcome and promotes tumor progression in colorectal carcinoma.
Yang L, Lin XL, Liang W, Fu SW, Lin WF, Tian XQ, Gao YJ, Chen HY, Dai J, Ge ZZ
Oncotarget8(29)47943-47956(2017 Jul)

International Union of Basic and Clinical Pharmacology. XCIV. Adhesion G protein-coupled receptors.
Hamann J, Aust G, Ara? D, Engel FB, Formstone C, Fredriksson R, Hall RA, Harty BL, Kirchhoff C, Knapp B, Krishnan A, Liebscher I, Lin HH, Martinelli DC, Monk KR, Peeters MC, Piao X, Pr?mel S, Sch?neberg T, Schwartz TW, Singer K, Stacey M, Ushkaryov YA, Vallon M, Wolfrum U, Wright MW, Xu L, Langenhan T, Schi?th HB
Pharmacological reviews67(2)338-67(2015)

GPR116, an adhesion G-protein-coupled receptor, promotes breast cancer metastasis via the G?q-p63RhoGEF-Rho GTPase pathway.
Tang X, Jin R, Qu G, Wang X, Li Z, Yuan Z, Zhao C, Siwko S, Shi T, Wang P, Xiao J, Liu M, Luo J
Cancer research73(20)6206-18(2013 Oct)

Essential regulation of lung surfactant homeostasis by the orphan G protein-coupled receptor GPR116.
Yang MY, Hilton MB, Seaman S, Haines DC, Nagashima K, Burks CM, Tessarollo L, Ivanova PT, Brown HA, Umstead TM, Floros J, Chroneos ZC, St Croix B
Cell reports3(5)1457-64(2013 May)

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Bailey SD, Xie C, Do R, Montpetit A, Diaz R, Mohan V, Keavney B, Yusuf S, Gerstein HC, Engert JC, Anand S,
Diabetes care33(10)2250-3(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings show that GPR116 is crucial for the metastasis of breast cancer and support GPR116 as a potential prognostic marker and drug target against metastatic human breast cancer.
Title: GPR116, an adhesion G-protein-coupled receptor, promotes breast cancer metastasis via the G?q-p63RhoGEF-Rho GTPase pathway.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

Clinical trial and genome-wide association study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Molecular genetics of successful smoking cessation: convergent genome-wide association study results.

The following ADGRF5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADGRF5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu96383	XM_017010421.2 Latest version!	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu96383	XM_017010420.2 Latest version!	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu96384	XM_017010422.2 Latest version!	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu96385	XM_017010423.1 Latest version!	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu96386	XM_017010424.2 Latest version!	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu78035	XM_011514354.2 Latest version!	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu23485	NM_001098518.2 Latest version!	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$384.30 $549.00
OHu23485	NM_015234.5 Latest version!	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$384.30 $549.00
OHu40185	XM_005248893.3 Latest version!	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu40185	XM_005248892.3 Latest version!	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu40186	XM_005248894.4 Latest version!	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu40187	XM_017010418.2 Latest version!	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu40187	XM_005248895.4 Latest version!	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu78034	XM_017010419.2 Latest version!	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu78034	XM_011514353.3 Latest version!	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu23485	NM_015234.4	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$384.30 $549.00
OHu96386	XM_017010424.1	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu40186	XM_005248894.3	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu78034	XM_011514353.2	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu23485	NM_001098518.1	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$384.30 $549.00
OHu40185	XM_005248892.2	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu40187	XM_017010418.1	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu78034	XM_017010419.1	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu40187	XM_005248895.3	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu96383	XM_017010420.1	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu96383	XM_017010421.1	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu96384	XM_017010422.1	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ADGRF5 ( XM_017010421.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865910.1 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu96383
Clone ID Related Accession (Same CDS sequence)	XM_017010421.1 , XM_017010420.1 , XM_017010420.2 , XM_017010421.2
Accession Version	XM_017010421.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCCCCTAAGA TCCCCCCAGG CCATGACGGA AACCTTGTTA GCGGTGGCAG TTCAGCAATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865910.1
CDS	246..4268
Translation	MKSPRRTTLCLMFIVIYSSKAALNWNYESTIHPLSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEISFENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVCKLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTAPKIPPGHDGNLVSGGSSAI

Target ORF information:

RefSeq Version	XM_017010421.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010421.1

ORF Insert Sequence:

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCCCCTAAGA TCCCCCCAGG CCATGACGGA AACCTTGTTA GCGGTGGCAG TTCAGCAATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( XM_017010420.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865909.1 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu96383
Clone ID Related Accession (Same CDS sequence)	XM_017010421.1 , XM_017010420.1 , XM_017010420.2 , XM_017010421.2
Accession Version	XM_017010420.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCCCCTAAGA TCCCCCCAGG CCATGACGGA AACCTTGTTA GCGGTGGCAG TTCAGCAATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865909.1
CDS	290..4312
Translation	MKSPRRTTLCLMFIVIYSSKAALNWNYESTIHPLSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEISFENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVCKLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTAPKIPPGHDGNLVSGGSSAI

Target ORF information:

RefSeq Version	XM_017010420.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010420.1

ORF Insert Sequence:

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCCCCTAAGA TCCCCCCAGG CCATGACGGA AACCTTGTTA GCGGTGGCAG TTCAGCAATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( XM_017010420.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865909.1 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu96383
Clone ID Related Accession (Same CDS sequence)	XM_017010421.1 , XM_017010420.1 , XM_017010420.2 , XM_017010421.2
Accession Version	XM_017010420.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017010420.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCCCCTAAGA TCCCCCCAGG CCATGACGGA AACCTTGTTA GCGGTGGCAG TTCAGCAATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865909.1
CDS	277..4299
Translation	MKSPRRTTLCLMFIVIYSSKAALNWNYESTIHPLSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEISFENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVCKLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTAPKIPPGHDGNLVSGGSSAI

Target ORF information:

RefSeq Version	XM_017010420.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010420.2

ORF Insert Sequence:

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCCCCTAAGA TCCCCCCAGG CCATGACGGA AACCTTGTTA GCGGTGGCAG TTCAGCAATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( XM_017010421.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865910.1 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X9, mRNA.

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CloneID	OHu96383
Clone ID Related Accession (Same CDS sequence)	XM_017010421.1 , XM_017010420.1 , XM_017010420.2 , XM_017010421.2
Accession Version	XM_017010421.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017010421.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCCCCTAAGA TCCCCCCAGG CCATGACGGA AACCTTGTTA GCGGTGGCAG TTCAGCAATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865910.1
CDS	246..4268
Translation	MKSPRRTTLCLMFIVIYSSKAALNWNYESTIHPLSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEISFENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVCKLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTAPKIPPGHDGNLVSGGSSAI

Target ORF information:

RefSeq Version	XM_017010421.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010421.2

ORF Insert Sequence:

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCCCCTAAGA TCCCCCCAGG CCATGACGGA AACCTTGTTA GCGGTGGCAG TTCAGCAATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( XM_017010422.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865911.1 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X10, mRNA.

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CloneID	OHu96384
Clone ID Related Accession (Same CDS sequence)	XM_017010422.1 , XM_017010422.2
Accession Version	XM_017010422.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGAAC CCCTGAGACA GACATAGATT CTAGCTGCAG CAGATACACC 
CTCAAGGCTG ATGGAACCCA GTGCCCAAGC GGGTCGTCTG GAACAACAGT CATCTACACT 
TGTGAGTTCA TCAGTGCCTA TGGAGCCAGA GGCAGTGCAA ACATAAAAGT GACATTCATC 
TCTGTGGGAG ATAATTCCAG CCACAAAAGT GAAGTGCACG AGGTCCTAAG GCATGTGATG 
GAAATGAGTA CAGTGCAGAG CACCACCAGG AACAGCCTAG AAACGAGTAA AGCCCAGGAG 
CAGCAAGCCA ATCTAACAAT AACCCCGGAC CCAATTTCTG TTTCTGAGGG ACAAAACTTT 
TCTATAAAAT GCATCAGTGA TGTGAGTAAC TATGATGAGG TTTATTGGAA CACTTCTGCT 
GGAATTAAAA TATACCAAAG ATTTTATACC ACGAGGAGGT ATCTTGATGG AGCAGAATCA 
GTACTGACAG TCAAGACCTC GACCAGGGAG TGGAATGGAA CCTATCACTG CATATTTAGA 
TATAAGAATT CATACAGTAT TGCAACCAAA GACGTCATTG TTCACCCGCT GCCTCTAAAG 
CTGAACATCA TGGTTGATCC TTTGGAAGCT ACTGTTTCAT GCAGTGGTTC CCATCACATC 
AAGTGCTGCA TAGAGGAGGA TGGAGACTAC AAAGTTACTT TCCATACGGG TTCCTCATCC 
CTTCCTGCTG CAAAAGAAGT TAACAAAAAA CAAGTGTGCT ACAAACACAA TTTCAATGCA 
AGCTCAGTTT CCTGGTGTTC AAAAACTGTT GATGTGTGTT GTCACTTTAC CAATGCTGCT 
AATAATTCAG TCTGGAGCCC ATCTATGAAG CTGAATCTGG TTCCTGGGGA AAACATCACA 
TGCCAGGATC CCGTAATAGG TGTCGGAGAG CCGGGGAAAG TCATCCAGAA GCTATGCCGG 
TTCTCAAACG TTCCCAGCAG CCCTGAGAGT CCCATTGGCG GGACCATCAC TTACAAATGT 
GTAGGCTCCC AGTGGGAGGA GAAGAGAAAT GACTGCATCT CTGCCCCAAT AAACAGTCTG 
CTCCAGATGG CTAAGGCTTT GATCAAGAGC CCCTCTCAGG ATGAGATGCT CCCTACATAC 
CTGAAGGATC TTTCTATTAG CATAGACAAA GCGGAACATG AAATCAGCTC TTCTCCTGGG 
AGTCTGGGAG CCATTATTAA CATCCTTGAT CTGCTCTCAA CAGTTCCAAC CCAAGTAAAT 
TCAGAAATGA TGACGCACGT GCTCTCTACG GTTAATGTCA TCCTTGGCAA GCCCGTCTTG 
AACACCTGGA AGGTTTTACA ACAGCAATGG ACCAATCAGA GTTCACAGCT ACTACATTCA 
GTGGAAAGAT TTTCCCAAGC ATTACAGTCG GGAGATAGCC CTCCTTTGTC CTTCTCCCAA 
ACTAATGTGC AGATGAGCAG CATGGTAATC AAGTCCAGCC ACCCAGAAAC CTATCAACAG 
AGGTTTGTTT TCCCATACTT TGACCTCTGG GGCAATGTGG TCATTGACAA GAGCTATCTA 
GAAAACTTGC AGTCGGATTC GTCTATTGTC ACCATGGCTT TCCCAACTCT CCAAGCCATC 
CTTGCCCAGG ATATCCAGGA AAATAACTTT GCAGAGAGCT TAGTGATGAC AACCACTGTC 
AGCCACAATA CAACTATGCC ATTCAGGATT TCAATGACTT TTAAGAACAA TAGCCCTTCA 
GGCGGCGAAA CGAAGTGTGT CTTCTGGAAC TTCAGGCTTG CCAACAACAC AGGGGGGTGG 
GACAGCAGTG GGTGCTATGT AGAAGAAGGT GATGGGGACA ATGTCACCTG TATCTGTGAC 
CACCTAACAT CATTCTCCAT CCTCATGTCC CCTGACTCCC CAGATCCTAG TTCTCTCCTG 
GGAATACTCC TGGATATTAT TTCTTATGTT GGGGTGGGCT TTTCCATCTT GAGCTTGGCA 
GCCTGTCTAG TTGTGGAAGC TGTGGTGTGG AAATCGGTGA CCAAGAACCG GACTTCTTAT 
ATGCGCCACA CCTGCATAGT GAATATCGCT GCCTCCCTTC TGGTCGCCAA CACCTGGTTC 
ATTGTGGTCG CTGCCATCCA GGACAATCGC TACATACTCT GCAAGACAGC CTGTGTGGCT 
GCCACCTTCT TCATCCACTT CTTCTACCTC AGCGTCTTCT TCTGGATGCT GACACTGGGC 
CTCATGCTGT TCTATCGCCT GGTTTTCATT CTGCATGAAA CAAGCAGGTC CACTCAGAAA 
GCCATTGCCT TCTGTCTTGG CTATGGCTGC CCACTTGCCA TCTCGGTCAT CACGCTGGGA 
GCCACCCAGC CCCGGGAAGT CTATACGAGG AAGAATGTCT GTTGGCTCAA CTGGGAGGAC 
ACCAAGGCCC TGCTGGCTTT CGCCATCCCA GCACTGATCA TTGTGGTGGT GAACATAACC 
ATCACTATTG TGGTCATCAC CAAGATCCTG AGGCCTTCCA TTGGAGACAA GCCATGCAAG 
CAGGAGAAGA GCAGCCTGTT TCAGATCAGC AAGAGCATTG GGGTCCTCAC ACCACTCTTG 
GGCCTCACTT GGGGTTTTGG TCTCACCACT GTGTTCCCAG GGACCAACCT TGTGTTCCAT 
ATCATATTTG CCATCCTCAA TGTCTTCCAG GGATTATTCA TTTTACTCTT TGGATGCCTC 
TGGGATCTGA AGGTACAGGA AGCTTTGCTG AATAAGTTTT CATTGTCGAG ATGGTCTTCA 
CAGCACTCAA AGTCAACATC CCTGGGTTCA TCCACACCTG TGTTTTCTAT GAGTTCTCCA 
ATATCAAGGA GATTTAACAA TTTGTTTGGT AAAACAGGAA CGTATAATGT TTCCACCCCA 
GAAGCAACCA GCTCATCCCT GGAAAACTCA TCCAGTGCTT CTTCGTTGCT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865911.1
CDS	290..4246
Translation	MKSPRRTTLCLMFIVIYSSKAALNWNYESTIHPLSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEISFENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVGDNSSHKSEVHEVLRHVMEMSTVQSTTRNSLETSKAQEQQANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPEATSSSLENSSSASSLLN

Target ORF information:

RefSeq Version	XM_017010422.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010422.1

ORF Insert Sequence:

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGAAC CCCTGAGACA GACATAGATT CTAGCTGCAG CAGATACACC 
CTCAAGGCTG ATGGAACCCA GTGCCCAAGC GGGTCGTCTG GAACAACAGT CATCTACACT 
TGTGAGTTCA TCAGTGCCTA TGGAGCCAGA GGCAGTGCAA ACATAAAAGT GACATTCATC 
TCTGTGGGAG ATAATTCCAG CCACAAAAGT GAAGTGCACG AGGTCCTAAG GCATGTGATG 
GAAATGAGTA CAGTGCAGAG CACCACCAGG AACAGCCTAG AAACGAGTAA AGCCCAGGAG 
CAGCAAGCCA ATCTAACAAT AACCCCGGAC CCAATTTCTG TTTCTGAGGG ACAAAACTTT 
TCTATAAAAT GCATCAGTGA TGTGAGTAAC TATGATGAGG TTTATTGGAA CACTTCTGCT 
GGAATTAAAA TATACCAAAG ATTTTATACC ACGAGGAGGT ATCTTGATGG AGCAGAATCA 
GTACTGACAG TCAAGACCTC GACCAGGGAG TGGAATGGAA CCTATCACTG CATATTTAGA 
TATAAGAATT CATACAGTAT TGCAACCAAA GACGTCATTG TTCACCCGCT GCCTCTAAAG 
CTGAACATCA TGGTTGATCC TTTGGAAGCT ACTGTTTCAT GCAGTGGTTC CCATCACATC 
AAGTGCTGCA TAGAGGAGGA TGGAGACTAC AAAGTTACTT TCCATACGGG TTCCTCATCC 
CTTCCTGCTG CAAAAGAAGT TAACAAAAAA CAAGTGTGCT ACAAACACAA TTTCAATGCA 
AGCTCAGTTT CCTGGTGTTC AAAAACTGTT GATGTGTGTT GTCACTTTAC CAATGCTGCT 
AATAATTCAG TCTGGAGCCC ATCTATGAAG CTGAATCTGG TTCCTGGGGA AAACATCACA 
TGCCAGGATC CCGTAATAGG TGTCGGAGAG CCGGGGAAAG TCATCCAGAA GCTATGCCGG 
TTCTCAAACG TTCCCAGCAG CCCTGAGAGT CCCATTGGCG GGACCATCAC TTACAAATGT 
GTAGGCTCCC AGTGGGAGGA GAAGAGAAAT GACTGCATCT CTGCCCCAAT AAACAGTCTG 
CTCCAGATGG CTAAGGCTTT GATCAAGAGC CCCTCTCAGG ATGAGATGCT CCCTACATAC 
CTGAAGGATC TTTCTATTAG CATAGACAAA GCGGAACATG AAATCAGCTC TTCTCCTGGG 
AGTCTGGGAG CCATTATTAA CATCCTTGAT CTGCTCTCAA CAGTTCCAAC CCAAGTAAAT 
TCAGAAATGA TGACGCACGT GCTCTCTACG GTTAATGTCA TCCTTGGCAA GCCCGTCTTG 
AACACCTGGA AGGTTTTACA ACAGCAATGG ACCAATCAGA GTTCACAGCT ACTACATTCA 
GTGGAAAGAT TTTCCCAAGC ATTACAGTCG GGAGATAGCC CTCCTTTGTC CTTCTCCCAA 
ACTAATGTGC AGATGAGCAG CATGGTAATC AAGTCCAGCC ACCCAGAAAC CTATCAACAG 
AGGTTTGTTT TCCCATACTT TGACCTCTGG GGCAATGTGG TCATTGACAA GAGCTATCTA 
GAAAACTTGC AGTCGGATTC GTCTATTGTC ACCATGGCTT TCCCAACTCT CCAAGCCATC 
CTTGCCCAGG ATATCCAGGA AAATAACTTT GCAGAGAGCT TAGTGATGAC AACCACTGTC 
AGCCACAATA CAACTATGCC ATTCAGGATT TCAATGACTT TTAAGAACAA TAGCCCTTCA 
GGCGGCGAAA CGAAGTGTGT CTTCTGGAAC TTCAGGCTTG CCAACAACAC AGGGGGGTGG 
GACAGCAGTG GGTGCTATGT AGAAGAAGGT GATGGGGACA ATGTCACCTG TATCTGTGAC 
CACCTAACAT CATTCTCCAT CCTCATGTCC CCTGACTCCC CAGATCCTAG TTCTCTCCTG 
GGAATACTCC TGGATATTAT TTCTTATGTT GGGGTGGGCT TTTCCATCTT GAGCTTGGCA 
GCCTGTCTAG TTGTGGAAGC TGTGGTGTGG AAATCGGTGA CCAAGAACCG GACTTCTTAT 
ATGCGCCACA CCTGCATAGT GAATATCGCT GCCTCCCTTC TGGTCGCCAA CACCTGGTTC 
ATTGTGGTCG CTGCCATCCA GGACAATCGC TACATACTCT GCAAGACAGC CTGTGTGGCT 
GCCACCTTCT TCATCCACTT CTTCTACCTC AGCGTCTTCT TCTGGATGCT GACACTGGGC 
CTCATGCTGT TCTATCGCCT GGTTTTCATT CTGCATGAAA CAAGCAGGTC CACTCAGAAA 
GCCATTGCCT TCTGTCTTGG CTATGGCTGC CCACTTGCCA TCTCGGTCAT CACGCTGGGA 
GCCACCCAGC CCCGGGAAGT CTATACGAGG AAGAATGTCT GTTGGCTCAA CTGGGAGGAC 
ACCAAGGCCC TGCTGGCTTT CGCCATCCCA GCACTGATCA TTGTGGTGGT GAACATAACC 
ATCACTATTG TGGTCATCAC CAAGATCCTG AGGCCTTCCA TTGGAGACAA GCCATGCAAG 
CAGGAGAAGA GCAGCCTGTT TCAGATCAGC AAGAGCATTG GGGTCCTCAC ACCACTCTTG 
GGCCTCACTT GGGGTTTTGG TCTCACCACT GTGTTCCCAG GGACCAACCT TGTGTTCCAT 
ATCATATTTG CCATCCTCAA TGTCTTCCAG GGATTATTCA TTTTACTCTT TGGATGCCTC 
TGGGATCTGA AGGTACAGGA AGCTTTGCTG AATAAGTTTT CATTGTCGAG ATGGTCTTCA 
CAGCACTCAA AGTCAACATC CCTGGGTTCA TCCACACCTG TGTTTTCTAT GAGTTCTCCA 
ATATCAAGGA GATTTAACAA TTTGTTTGGT AAAACAGGAA CGTATAATGT TTCCACCCCA 
GAAGCAACCA GCTCATCCCT GGAAAACTCA TCCAGTGCTT CTTCGTTGCT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( XM_017010422.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865911.1 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu96384
Clone ID Related Accession (Same CDS sequence)	XM_017010422.1 , XM_017010422.2
Accession Version	XM_017010422.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017010422.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGAAC CCCTGAGACA GACATAGATT CTAGCTGCAG CAGATACACC 
CTCAAGGCTG ATGGAACCCA GTGCCCAAGC GGGTCGTCTG GAACAACAGT CATCTACACT 
TGTGAGTTCA TCAGTGCCTA TGGAGCCAGA GGCAGTGCAA ACATAAAAGT GACATTCATC 
TCTGTGGGAG ATAATTCCAG CCACAAAAGT GAAGTGCACG AGGTCCTAAG GCATGTGATG 
GAAATGAGTA CAGTGCAGAG CACCACCAGG AACAGCCTAG AAACGAGTAA AGCCCAGGAG 
CAGCAAGCCA ATCTAACAAT AACCCCGGAC CCAATTTCTG TTTCTGAGGG ACAAAACTTT 
TCTATAAAAT GCATCAGTGA TGTGAGTAAC TATGATGAGG TTTATTGGAA CACTTCTGCT 
GGAATTAAAA TATACCAAAG ATTTTATACC ACGAGGAGGT ATCTTGATGG AGCAGAATCA 
GTACTGACAG TCAAGACCTC GACCAGGGAG TGGAATGGAA CCTATCACTG CATATTTAGA 
TATAAGAATT CATACAGTAT TGCAACCAAA GACGTCATTG TTCACCCGCT GCCTCTAAAG 
CTGAACATCA TGGTTGATCC TTTGGAAGCT ACTGTTTCAT GCAGTGGTTC CCATCACATC 
AAGTGCTGCA TAGAGGAGGA TGGAGACTAC AAAGTTACTT TCCATACGGG TTCCTCATCC 
CTTCCTGCTG CAAAAGAAGT TAACAAAAAA CAAGTGTGCT ACAAACACAA TTTCAATGCA 
AGCTCAGTTT CCTGGTGTTC AAAAACTGTT GATGTGTGTT GTCACTTTAC CAATGCTGCT 
AATAATTCAG TCTGGAGCCC ATCTATGAAG CTGAATCTGG TTCCTGGGGA AAACATCACA 
TGCCAGGATC CCGTAATAGG TGTCGGAGAG CCGGGGAAAG TCATCCAGAA GCTATGCCGG 
TTCTCAAACG TTCCCAGCAG CCCTGAGAGT CCCATTGGCG GGACCATCAC TTACAAATGT 
GTAGGCTCCC AGTGGGAGGA GAAGAGAAAT GACTGCATCT CTGCCCCAAT AAACAGTCTG 
CTCCAGATGG CTAAGGCTTT GATCAAGAGC CCCTCTCAGG ATGAGATGCT CCCTACATAC 
CTGAAGGATC TTTCTATTAG CATAGACAAA GCGGAACATG AAATCAGCTC TTCTCCTGGG 
AGTCTGGGAG CCATTATTAA CATCCTTGAT CTGCTCTCAA CAGTTCCAAC CCAAGTAAAT 
TCAGAAATGA TGACGCACGT GCTCTCTACG GTTAATGTCA TCCTTGGCAA GCCCGTCTTG 
AACACCTGGA AGGTTTTACA ACAGCAATGG ACCAATCAGA GTTCACAGCT ACTACATTCA 
GTGGAAAGAT TTTCCCAAGC ATTACAGTCG GGAGATAGCC CTCCTTTGTC CTTCTCCCAA 
ACTAATGTGC AGATGAGCAG CATGGTAATC AAGTCCAGCC ACCCAGAAAC CTATCAACAG 
AGGTTTGTTT TCCCATACTT TGACCTCTGG GGCAATGTGG TCATTGACAA GAGCTATCTA 
GAAAACTTGC AGTCGGATTC GTCTATTGTC ACCATGGCTT TCCCAACTCT CCAAGCCATC 
CTTGCCCAGG ATATCCAGGA AAATAACTTT GCAGAGAGCT TAGTGATGAC AACCACTGTC 
AGCCACAATA CAACTATGCC ATTCAGGATT TCAATGACTT TTAAGAACAA TAGCCCTTCA 
GGCGGCGAAA CGAAGTGTGT CTTCTGGAAC TTCAGGCTTG CCAACAACAC AGGGGGGTGG 
GACAGCAGTG GGTGCTATGT AGAAGAAGGT GATGGGGACA ATGTCACCTG TATCTGTGAC 
CACCTAACAT CATTCTCCAT CCTCATGTCC CCTGACTCCC CAGATCCTAG TTCTCTCCTG 
GGAATACTCC TGGATATTAT TTCTTATGTT GGGGTGGGCT TTTCCATCTT GAGCTTGGCA 
GCCTGTCTAG TTGTGGAAGC TGTGGTGTGG AAATCGGTGA CCAAGAACCG GACTTCTTAT 
ATGCGCCACA CCTGCATAGT GAATATCGCT GCCTCCCTTC TGGTCGCCAA CACCTGGTTC 
ATTGTGGTCG CTGCCATCCA GGACAATCGC TACATACTCT GCAAGACAGC CTGTGTGGCT 
GCCACCTTCT TCATCCACTT CTTCTACCTC AGCGTCTTCT TCTGGATGCT GACACTGGGC 
CTCATGCTGT TCTATCGCCT GGTTTTCATT CTGCATGAAA CAAGCAGGTC CACTCAGAAA 
GCCATTGCCT TCTGTCTTGG CTATGGCTGC CCACTTGCCA TCTCGGTCAT CACGCTGGGA 
GCCACCCAGC CCCGGGAAGT CTATACGAGG AAGAATGTCT GTTGGCTCAA CTGGGAGGAC 
ACCAAGGCCC TGCTGGCTTT CGCCATCCCA GCACTGATCA TTGTGGTGGT GAACATAACC 
ATCACTATTG TGGTCATCAC CAAGATCCTG AGGCCTTCCA TTGGAGACAA GCCATGCAAG 
CAGGAGAAGA GCAGCCTGTT TCAGATCAGC AAGAGCATTG GGGTCCTCAC ACCACTCTTG 
GGCCTCACTT GGGGTTTTGG TCTCACCACT GTGTTCCCAG GGACCAACCT TGTGTTCCAT 
ATCATATTTG CCATCCTCAA TGTCTTCCAG GGATTATTCA TTTTACTCTT TGGATGCCTC 
TGGGATCTGA AGGTACAGGA AGCTTTGCTG AATAAGTTTT CATTGTCGAG ATGGTCTTCA 
CAGCACTCAA AGTCAACATC CCTGGGTTCA TCCACACCTG TGTTTTCTAT GAGTTCTCCA 
ATATCAAGGA GATTTAACAA TTTGTTTGGT AAAACAGGAA CGTATAATGT TTCCACCCCA 
GAAGCAACCA GCTCATCCCT GGAAAACTCA TCCAGTGCTT CTTCGTTGCT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865911.1
CDS	277..4233
Translation	MKSPRRTTLCLMFIVIYSSKAALNWNYESTIHPLSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEISFENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVGDNSSHKSEVHEVLRHVMEMSTVQSTTRNSLETSKAQEQQANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPEATSSSLENSSSASSLLN

Target ORF information:

RefSeq Version	XM_017010422.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010422.2

ORF Insert Sequence:

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGAAC CCCTGAGACA GACATAGATT CTAGCTGCAG CAGATACACC 
CTCAAGGCTG ATGGAACCCA GTGCCCAAGC GGGTCGTCTG GAACAACAGT CATCTACACT 
TGTGAGTTCA TCAGTGCCTA TGGAGCCAGA GGCAGTGCAA ACATAAAAGT GACATTCATC 
TCTGTGGGAG ATAATTCCAG CCACAAAAGT GAAGTGCACG AGGTCCTAAG GCATGTGATG 
GAAATGAGTA CAGTGCAGAG CACCACCAGG AACAGCCTAG AAACGAGTAA AGCCCAGGAG 
CAGCAAGCCA ATCTAACAAT AACCCCGGAC CCAATTTCTG TTTCTGAGGG ACAAAACTTT 
TCTATAAAAT GCATCAGTGA TGTGAGTAAC TATGATGAGG TTTATTGGAA CACTTCTGCT 
GGAATTAAAA TATACCAAAG ATTTTATACC ACGAGGAGGT ATCTTGATGG AGCAGAATCA 
GTACTGACAG TCAAGACCTC GACCAGGGAG TGGAATGGAA CCTATCACTG CATATTTAGA 
TATAAGAATT CATACAGTAT TGCAACCAAA GACGTCATTG TTCACCCGCT GCCTCTAAAG 
CTGAACATCA TGGTTGATCC TTTGGAAGCT ACTGTTTCAT GCAGTGGTTC CCATCACATC 
AAGTGCTGCA TAGAGGAGGA TGGAGACTAC AAAGTTACTT TCCATACGGG TTCCTCATCC 
CTTCCTGCTG CAAAAGAAGT TAACAAAAAA CAAGTGTGCT ACAAACACAA TTTCAATGCA 
AGCTCAGTTT CCTGGTGTTC AAAAACTGTT GATGTGTGTT GTCACTTTAC CAATGCTGCT 
AATAATTCAG TCTGGAGCCC ATCTATGAAG CTGAATCTGG TTCCTGGGGA AAACATCACA 
TGCCAGGATC CCGTAATAGG TGTCGGAGAG CCGGGGAAAG TCATCCAGAA GCTATGCCGG 
TTCTCAAACG TTCCCAGCAG CCCTGAGAGT CCCATTGGCG GGACCATCAC TTACAAATGT 
GTAGGCTCCC AGTGGGAGGA GAAGAGAAAT GACTGCATCT CTGCCCCAAT AAACAGTCTG 
CTCCAGATGG CTAAGGCTTT GATCAAGAGC CCCTCTCAGG ATGAGATGCT CCCTACATAC 
CTGAAGGATC TTTCTATTAG CATAGACAAA GCGGAACATG AAATCAGCTC TTCTCCTGGG 
AGTCTGGGAG CCATTATTAA CATCCTTGAT CTGCTCTCAA CAGTTCCAAC CCAAGTAAAT 
TCAGAAATGA TGACGCACGT GCTCTCTACG GTTAATGTCA TCCTTGGCAA GCCCGTCTTG 
AACACCTGGA AGGTTTTACA ACAGCAATGG ACCAATCAGA GTTCACAGCT ACTACATTCA 
GTGGAAAGAT TTTCCCAAGC ATTACAGTCG GGAGATAGCC CTCCTTTGTC CTTCTCCCAA 
ACTAATGTGC AGATGAGCAG CATGGTAATC AAGTCCAGCC ACCCAGAAAC CTATCAACAG 
AGGTTTGTTT TCCCATACTT TGACCTCTGG GGCAATGTGG TCATTGACAA GAGCTATCTA 
GAAAACTTGC AGTCGGATTC GTCTATTGTC ACCATGGCTT TCCCAACTCT CCAAGCCATC 
CTTGCCCAGG ATATCCAGGA AAATAACTTT GCAGAGAGCT TAGTGATGAC AACCACTGTC 
AGCCACAATA CAACTATGCC ATTCAGGATT TCAATGACTT TTAAGAACAA TAGCCCTTCA 
GGCGGCGAAA CGAAGTGTGT CTTCTGGAAC TTCAGGCTTG CCAACAACAC AGGGGGGTGG 
GACAGCAGTG GGTGCTATGT AGAAGAAGGT GATGGGGACA ATGTCACCTG TATCTGTGAC 
CACCTAACAT CATTCTCCAT CCTCATGTCC CCTGACTCCC CAGATCCTAG TTCTCTCCTG 
GGAATACTCC TGGATATTAT TTCTTATGTT GGGGTGGGCT TTTCCATCTT GAGCTTGGCA 
GCCTGTCTAG TTGTGGAAGC TGTGGTGTGG AAATCGGTGA CCAAGAACCG GACTTCTTAT 
ATGCGCCACA CCTGCATAGT GAATATCGCT GCCTCCCTTC TGGTCGCCAA CACCTGGTTC 
ATTGTGGTCG CTGCCATCCA GGACAATCGC TACATACTCT GCAAGACAGC CTGTGTGGCT 
GCCACCTTCT TCATCCACTT CTTCTACCTC AGCGTCTTCT TCTGGATGCT GACACTGGGC 
CTCATGCTGT TCTATCGCCT GGTTTTCATT CTGCATGAAA CAAGCAGGTC CACTCAGAAA 
GCCATTGCCT TCTGTCTTGG CTATGGCTGC CCACTTGCCA TCTCGGTCAT CACGCTGGGA 
GCCACCCAGC CCCGGGAAGT CTATACGAGG AAGAATGTCT GTTGGCTCAA CTGGGAGGAC 
ACCAAGGCCC TGCTGGCTTT CGCCATCCCA GCACTGATCA TTGTGGTGGT GAACATAACC 
ATCACTATTG TGGTCATCAC CAAGATCCTG AGGCCTTCCA TTGGAGACAA GCCATGCAAG 
CAGGAGAAGA GCAGCCTGTT TCAGATCAGC AAGAGCATTG GGGTCCTCAC ACCACTCTTG 
GGCCTCACTT GGGGTTTTGG TCTCACCACT GTGTTCCCAG GGACCAACCT TGTGTTCCAT 
ATCATATTTG CCATCCTCAA TGTCTTCCAG GGATTATTCA TTTTACTCTT TGGATGCCTC 
TGGGATCTGA AGGTACAGGA AGCTTTGCTG AATAAGTTTT CATTGTCGAG ATGGTCTTCA 
CAGCACTCAA AGTCAACATC CCTGGGTTCA TCCACACCTG TGTTTTCTAT GAGTTCTCCA 
ATATCAAGGA GATTTAACAA TTTGTTTGGT AAAACAGGAA CGTATAATGT TTCCACCCCA 
GAAGCAACCA GCTCATCCCT GGAAAACTCA TCCAGTGCTT CTTCGTTGCT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( XM_017010423.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865912.1 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OHu96385
Clone ID Related Accession (Same CDS sequence)	XM_017010423.1
Accession Version	XM_017010423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGTCA GACTAAATGT AGGCTTTCAA GAAGACCTCA TGAACACTTC CTCCGCCCTC 
TATAGGTCCT ACAAGACCGA CTTGGAAACA GCGTTCCGGA AGGGTTACGG AATTTTACCA 
GGCTTCAAGG GCGTGACTGT GACAGGGTTC AAGTCTGGAA GTGTGGTTGT GACATATGAA 
GTCAAGACTA CACCACCATC ACTTGAGTTA ATACATAAAG CCAATGAACA AGTTGTACAG 
AGCCTCAATC AGACCTACAA AATGGACTAC AACTCCTTTC AAGCAGTTAC TATCAATGAA 
AGCAATTTCT TTGTCACACC AGAAATCATC TTTGAAGGGG ACACAGTCAG TCTGGTGTGT 
GAAAAGGAAG TTTTGTCCTC CAATGTGTCT TGGCGCTATG AAGAACAGCA GTTGGAAATC 
CAGAACAGCA GCAGATTCTC GATTTACACC GCACTTTTCA ACAACATGAC TTCGGTGTCC 
AAGCTCACCA TCCACAACAT CACTCCAGGT GATGCAGGTG AATATGTTTG CAAACTGATA 
TTAGACATTT TTGAATATGA GTGCAAGAAG AAAATAGATG TTATGCCCAT CCAAATTTTG 
GCAAATGAAG AAATGAAGGT GATGTGCGAC AACAATCCTG TATCTTTGAA CTGCTGCAGT 
CAGGGTAATG TTAATTGGAG CAAAGTAGAA TGGAAGCAGG AAGGAAAAAT AAATATTCCA 
GGAACCCCTG AGACAGACAT AGATTCTAGC TGCAGCAGAT ACACCCTCAA GGCTGATGGA 
ACCCAGTGCC CAAGCGGGTC GTCTGGAACA ACAGTCATCT ACACTTGTGA GTTCATCAGT 
GCCTATGGAG CCAGAGGCAG TGCAAACATA AAAGTGACAT TCATCTCTGT GGCCAATCTA 
ACAATAACCC CGGACCCAAT TTCTGTTTCT GAGGGACAAA ACTTTTCTAT AAAATGCATC 
AGTGATGTGA GTAACTATGA TGAGGTTTAT TGGAACACTT CTGCTGGAAT TAAAATATAC 
CAAAGATTTT ATACCACGAG GAGGTATCTT GATGGAGCAG AATCAGTACT GACAGTCAAG 
ACCTCGACCA GGGAGTGGAA TGGAACCTAT CACTGCATAT TTAGATATAA GAATTCATAC 
AGTATTGCAA CCAAAGACGT CATTGTTCAC CCGCTGCCTC TAAAGCTGAA CATCATGGTT 
GATCCTTTGG AAGCTACTGT TTCATGCAGT GGTTCCCATC ACATCAAGTG CTGCATAGAG 
GAGGATGGAG ACTACAAAGT TACTTTCCAT ACGGGTTCCT CATCCCTTCC TGCTGCAAAA 
GAAGTTAACA AAAAACAAGT GTGCTACAAA CACAATTTCA ATGCAAGCTC AGTTTCCTGG 
TGTTCAAAAA CTGTTGATGT GTGTTGTCAC TTTACCAATG CTGCTAATAA TTCAGTCTGG 
AGCCCATCTA TGAAGCTGAA TCTGGTTCCT GGGGAAAACA TCACATGCCA GGATCCCGTA 
ATAGGTGTCG GAGAGCCGGG GAAAGTCATC CAGAAGCTAT GCCGGTTCTC AAACGTTCCC 
AGCAGCCCTG AGAGTCCCAT TGGCGGGACC ATCACTTACA AATGTGTAGG CTCCCAGTGG 
GAGGAGAAGA GAAATGACTG CATCTCTGCC CCAATAAACA GTCTGCTCCA GATGGCTAAG 
GCTTTGATCA AGAGCCCCTC TCAGGATGAG ATGCTCCCTA CATACCTGAA GGATCTTTCT 
ATTAGCATAG ACAAAGCGGA ACATGAAATC AGCTCTTCTC CTGGGAGTCT GGGAGCCATT 
ATTAACATCC TTGATCTGCT CTCAACAGTT CCAACCCAAG TAAATTCAGA AATGATGACG 
CACGTGCTCT CTACGGTTAA TGTCATCCTT GGCAAGCCCG TCTTGAACAC CTGGAAGGTT 
TTACAACAGC AATGGACCAA TCAGAGTTCA CAGCTACTAC ATTCAGTGGA AAGATTTTCC 
CAAGCATTAC AGTCGGGAGA TAGCCCTCCT TTGTCCTTCT CCCAAACTAA TGTGCAGATG 
AGCAGCATGG TAATCAAGTC CAGCCACCCA GAAACCTATC AACAGAGGTT TGTTTTCCCA 
TACTTTGACC TCTGGGGCAA TGTGGTCATT GACAAGAGCT ATCTAGAAAA CTTGCAGTCG 
GATTCGTCTA TTGTCACCAT GGCTTTCCCA ACTCTCCAAG CCATCCTTGC CCAGGATATC 
CAGGAAAATA ACTTTGCAGA GAGCTTAGTG ATGACAACCA CTGTCAGCCA CAATACAACT 
ATGCCATTCA GGATTTCAAT GACTTTTAAG AACAATAGCC CTTCAGGCGG CGAAACGAAG 
TGTGTCTTCT GGAACTTCAG GCTTGCCAAC AACACAGGGG GGTGGGACAG CAGTGGGTGC 
TATGTAGAAG AAGGTGATGG GGACAATGTC ACCTGTATCT GTGACCACCT AACATCATTC 
TCCATCCTCA TGTCCCCTGA CTCCCCAGAT CCTAGTTCTC TCCTGGGAAT ACTCCTGGAT 
ATTATTTCTT ATGTTGGGGT GGGCTTTTCC ATCTTGAGCT TGGCAGCCTG TCTAGTTGTG 
GAAGCTGTGG TGTGGAAATC GGTGACCAAG AACCGGACTT CTTATATGCG CCACACCTGC 
ATAGTGAATA TCGCTGCCTC CCTTCTGGTC GCCAACACCT GGTTCATTGT GGTCGCTGCC 
ATCCAGGACA ATCGCTACAT ACTCTGCAAG ACAGCCTGTG TGGCTGCCAC CTTCTTCATC 
CACTTCTTCT ACCTCAGCGT CTTCTTCTGG ATGCTGACAC TGGGCCTCAT GCTGTTCTAT 
CGCCTGGTTT TCATTCTGCA TGAAACAAGC AGGTCCACTC AGAAAGCCAT TGCCTTCTGT 
CTTGGCTATG GCTGCCCACT TGCCATCTCG GTCATCACGC TGGGAGCCAC CCAGCCCCGG 
GAAGTCTATA CGAGGAAGAA TGTCTGTTGG CTCAACTGGG AGGACACCAA GGCCCTGCTG 
GCTTTCGCCA TCCCAGCACT GATCATTGTG GTGGTGAACA TAACCATCAC TATTGTGGTC 
ATCACCAAGA TCCTGAGGCC TTCCATTGGA GACAAGCCAT GCAAGCAGGA GAAGAGCAGC 
CTGTTTCAGA TCAGCAAGAG CATTGGGGTC CTCACACCAC TCTTGGGCCT CACTTGGGGT 
TTTGGTCTCA CCACTGTGTT CCCAGGGACC AACCTTGTGT TCCATATCAT ATTTGCCATC 
CTCAATGTCT TCCAGGGATT ATTCATTTTA CTCTTTGGAT GCCTCTGGGA TCTGAAGGTA 
CAGGAAGCTT TGCTGAATAA GTTTTCATTG TCGAGATGGT CTTCACAGCA CTCAAAGTCA 
ACATCCCTGG GTTCATCCAC ACCTGTGTTT TCTATGAGTT CTCCAATATC AAGGAGATTT 
AACAATTTGT TTGGTAAAAC AGGAACGTAT AATGTTTCCA CCCCAGAAGC AACCAGCTCA 
TCCCTGGAAA ACTCATCCAG TGCTTCTTCG TTGCTCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865912.1
CDS	832..4353
Translation	MRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVCKLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPEATSSSLENSSSASSLLN

Target ORF information:

RefSeq Version	XM_017010423.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010423.1

ORF Insert Sequence:

ATGAGAGTCA GACTAAATGT AGGCTTTCAA GAAGACCTCA TGAACACTTC CTCCGCCCTC 
TATAGGTCCT ACAAGACCGA CTTGGAAACA GCGTTCCGGA AGGGTTACGG AATTTTACCA 
GGCTTCAAGG GCGTGACTGT GACAGGGTTC AAGTCTGGAA GTGTGGTTGT GACATATGAA 
GTCAAGACTA CACCACCATC ACTTGAGTTA ATACATAAAG CCAATGAACA AGTTGTACAG 
AGCCTCAATC AGACCTACAA AATGGACTAC AACTCCTTTC AAGCAGTTAC TATCAATGAA 
AGCAATTTCT TTGTCACACC AGAAATCATC TTTGAAGGGG ACACAGTCAG TCTGGTGTGT 
GAAAAGGAAG TTTTGTCCTC CAATGTGTCT TGGCGCTATG AAGAACAGCA GTTGGAAATC 
CAGAACAGCA GCAGATTCTC GATTTACACC GCACTTTTCA ACAACATGAC TTCGGTGTCC 
AAGCTCACCA TCCACAACAT CACTCCAGGT GATGCAGGTG AATATGTTTG CAAACTGATA 
TTAGACATTT TTGAATATGA GTGCAAGAAG AAAATAGATG TTATGCCCAT CCAAATTTTG 
GCAAATGAAG AAATGAAGGT GATGTGCGAC AACAATCCTG TATCTTTGAA CTGCTGCAGT 
CAGGGTAATG TTAATTGGAG CAAAGTAGAA TGGAAGCAGG AAGGAAAAAT AAATATTCCA 
GGAACCCCTG AGACAGACAT AGATTCTAGC TGCAGCAGAT ACACCCTCAA GGCTGATGGA 
ACCCAGTGCC CAAGCGGGTC GTCTGGAACA ACAGTCATCT ACACTTGTGA GTTCATCAGT 
GCCTATGGAG CCAGAGGCAG TGCAAACATA AAAGTGACAT TCATCTCTGT GGCCAATCTA 
ACAATAACCC CGGACCCAAT TTCTGTTTCT GAGGGACAAA ACTTTTCTAT AAAATGCATC 
AGTGATGTGA GTAACTATGA TGAGGTTTAT TGGAACACTT CTGCTGGAAT TAAAATATAC 
CAAAGATTTT ATACCACGAG GAGGTATCTT GATGGAGCAG AATCAGTACT GACAGTCAAG 
ACCTCGACCA GGGAGTGGAA TGGAACCTAT CACTGCATAT TTAGATATAA GAATTCATAC 
AGTATTGCAA CCAAAGACGT CATTGTTCAC CCGCTGCCTC TAAAGCTGAA CATCATGGTT 
GATCCTTTGG AAGCTACTGT TTCATGCAGT GGTTCCCATC ACATCAAGTG CTGCATAGAG 
GAGGATGGAG ACTACAAAGT TACTTTCCAT ACGGGTTCCT CATCCCTTCC TGCTGCAAAA 
GAAGTTAACA AAAAACAAGT GTGCTACAAA CACAATTTCA ATGCAAGCTC AGTTTCCTGG 
TGTTCAAAAA CTGTTGATGT GTGTTGTCAC TTTACCAATG CTGCTAATAA TTCAGTCTGG 
AGCCCATCTA TGAAGCTGAA TCTGGTTCCT GGGGAAAACA TCACATGCCA GGATCCCGTA 
ATAGGTGTCG GAGAGCCGGG GAAAGTCATC CAGAAGCTAT GCCGGTTCTC AAACGTTCCC 
AGCAGCCCTG AGAGTCCCAT TGGCGGGACC ATCACTTACA AATGTGTAGG CTCCCAGTGG 
GAGGAGAAGA GAAATGACTG CATCTCTGCC CCAATAAACA GTCTGCTCCA GATGGCTAAG 
GCTTTGATCA AGAGCCCCTC TCAGGATGAG ATGCTCCCTA CATACCTGAA GGATCTTTCT 
ATTAGCATAG ACAAAGCGGA ACATGAAATC AGCTCTTCTC CTGGGAGTCT GGGAGCCATT 
ATTAACATCC TTGATCTGCT CTCAACAGTT CCAACCCAAG TAAATTCAGA AATGATGACG 
CACGTGCTCT CTACGGTTAA TGTCATCCTT GGCAAGCCCG TCTTGAACAC CTGGAAGGTT 
TTACAACAGC AATGGACCAA TCAGAGTTCA CAGCTACTAC ATTCAGTGGA AAGATTTTCC 
CAAGCATTAC AGTCGGGAGA TAGCCCTCCT TTGTCCTTCT CCCAAACTAA TGTGCAGATG 
AGCAGCATGG TAATCAAGTC CAGCCACCCA GAAACCTATC AACAGAGGTT TGTTTTCCCA 
TACTTTGACC TCTGGGGCAA TGTGGTCATT GACAAGAGCT ATCTAGAAAA CTTGCAGTCG 
GATTCGTCTA TTGTCACCAT GGCTTTCCCA ACTCTCCAAG CCATCCTTGC CCAGGATATC 
CAGGAAAATA ACTTTGCAGA GAGCTTAGTG ATGACAACCA CTGTCAGCCA CAATACAACT 
ATGCCATTCA GGATTTCAAT GACTTTTAAG AACAATAGCC CTTCAGGCGG CGAAACGAAG 
TGTGTCTTCT GGAACTTCAG GCTTGCCAAC AACACAGGGG GGTGGGACAG CAGTGGGTGC 
TATGTAGAAG AAGGTGATGG GGACAATGTC ACCTGTATCT GTGACCACCT AACATCATTC 
TCCATCCTCA TGTCCCCTGA CTCCCCAGAT CCTAGTTCTC TCCTGGGAAT ACTCCTGGAT 
ATTATTTCTT ATGTTGGGGT GGGCTTTTCC ATCTTGAGCT TGGCAGCCTG TCTAGTTGTG 
GAAGCTGTGG TGTGGAAATC GGTGACCAAG AACCGGACTT CTTATATGCG CCACACCTGC 
ATAGTGAATA TCGCTGCCTC CCTTCTGGTC GCCAACACCT GGTTCATTGT GGTCGCTGCC 
ATCCAGGACA ATCGCTACAT ACTCTGCAAG ACAGCCTGTG TGGCTGCCAC CTTCTTCATC 
CACTTCTTCT ACCTCAGCGT CTTCTTCTGG ATGCTGACAC TGGGCCTCAT GCTGTTCTAT 
CGCCTGGTTT TCATTCTGCA TGAAACAAGC AGGTCCACTC AGAAAGCCAT TGCCTTCTGT 
CTTGGCTATG GCTGCCCACT TGCCATCTCG GTCATCACGC TGGGAGCCAC CCAGCCCCGG 
GAAGTCTATA CGAGGAAGAA TGTCTGTTGG CTCAACTGGG AGGACACCAA GGCCCTGCTG 
GCTTTCGCCA TCCCAGCACT GATCATTGTG GTGGTGAACA TAACCATCAC TATTGTGGTC 
ATCACCAAGA TCCTGAGGCC TTCCATTGGA GACAAGCCAT GCAAGCAGGA GAAGAGCAGC 
CTGTTTCAGA TCAGCAAGAG CATTGGGGTC CTCACACCAC TCTTGGGCCT CACTTGGGGT 
TTTGGTCTCA CCACTGTGTT CCCAGGGACC AACCTTGTGT TCCATATCAT ATTTGCCATC 
CTCAATGTCT TCCAGGGATT ATTCATTTTA CTCTTTGGAT GCCTCTGGGA TCTGAAGGTA 
CAGGAAGCTT TGCTGAATAA GTTTTCATTG TCGAGATGGT CTTCACAGCA CTCAAAGTCA 
ACATCCCTGG GTTCATCCAC ACCTGTGTTT TCTATGAGTT CTCCAATATC AAGGAGATTT 
AACAATTTGT TTGGTAAAAC AGGAACGTAT AATGTTTCCA CCCCAGAAGC AACCAGCTCA 
TCCCTGGAAA ACTCATCCAG TGCTTCTTCG TTGCTCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( XM_017010424.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865913.1 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X13, mRNA.

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CloneID	OHu96386
Clone ID Related Accession (Same CDS sequence)	XM_017010424.1 , XM_017010424.2
Accession Version	XM_017010424.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGTCA GACTAAATGT AGGCTTTCAA GAAGACCTCA TGAACACTTC CTCCGCCCTC 
TATAGGTCCT ACAAGACCGA CTTGGAAACA GCGTTCCGGA AGGGTTACGG AATTTTACCA 
GGCTTCAAGG GCGTGACTGT GACAGGGTTC AAGTCTGGAA GTGTGGTTGT GACATATGAA 
GTCAAGACTA CACCACCATC ACTTGAGTTA ATACATAAAG CCAATGAACA AGTTGTACAG 
AGCCTCAATC AGACCTACAA AATGGACTAC AACTCCTTTC AAGCAGTTAC TATCAATGAA 
AGCAATTTCT TTGTCACACC AGAAATCATC TTTGAAGGGG ACACAGTCAG TCTGGTGTGT 
GAAAAGGAAG TTTTGTCCTC CAATGTGTCT TGGCGCTATG AAGAACAGCA GTTGGAAATC 
CAGAACAGCA GCAGATTCTC GATTTACACC GCACTTTTCA ACAACATGAC TTCGGTGTCC 
AAGCTCACCA TCCACAACAT CACTCCAGGT GATGCAGGTG AATATGTTTG CAAACTGATA 
TTAGACATTT TTGAATATGA GTGCAAGAAG AAAATAGATG TTATGCCCAT CCAAATTTTG 
GCAAATGAAG AAATGAAGGT GATGTGCGAC AACAATCCTG TATCTTTGAA CTGCTGCAGT 
CAGGGTAATG TTAATTGGAG CAAAGTAGAA TGGAAGCAGG AAGGAAAAAT AAATATTCCA 
GGAACCCCTG AGACAGACAT AGATTCTAGC TGCAGCAGAT ACACCCTCAA GGCTGATGGA 
ACCCAGTGCC CAAGCGGGTC GTCTGGAACA ACAGTCATCT ACACTTGTGA GTTCATCAGT 
GCCTATGGAG CCAGAGGCAG TGCAAACATA AAAGTGACAT TCATCTCTGT GGCCAATCTA 
ACAATAACCC CGGACCCAAT TTCTGTTTCT GAGGGACAAA ACTTTTCTAT AAAATGCATC 
AGTGATGTGA GTAACTATGA TGAGGTTTAT TGGAACACTT CTGCTGGAAT TAAAATATAC 
CAAAGATTTT ATACCACGAG GAGGTATCTT GATGGAGCAG AATCAGTACT GACAGTCAAG 
ACCTCGACCA GGGAGTGGAA TGGAACCTAT CACTGCATAT TTAGATATAA GAATTCATAC 
AGTATTGCAA CCAAAGACGT CATTGTTCAC CCGCTGCCTC TAAAGCTGAA CATCATGGTT 
GATCCTTTGG AAGCTACTGT TTCATGCAGT GGTTCCCATC ACATCAAGTG CTGCATAGAG 
GAGGATGGAG ACTACAAAGT TACTTTCCAT ACGGGTTCCT CATCCCTTCC TGCTGCAAAA 
GAAGTTAACA AAAAACAAGT GTGCTACAAA CACAATTTCA ATGCAAGCTC AGTTTCCTGG 
TGTTCAAAAA CTGTTGATGT GTGTTGTCAC TTTACCAATG CTGCTAATAA TTCAGTCTGG 
AGCCCATCTA TGAAGCTGAA TCTGGTTCCT GGGGAAAACA TCACATGCCA GGATCCCGTA 
ATAGGTGTCG GAGAGCCGGG GAAAGTCATC CAGAAGCTAT GCCGGTTCTC AAACGTTCCC 
AGCAGCCCTG AGAGTCCCAT TGGCGGGACC ATCACTTACA AATGTGTAGG CTCCCAGTGG 
GAGGAGAAGA GAAATGACTG CATCTCTGCC CCAATAAACA GTCTGCTCCA GATGGCTAAG 
GCTTTGATCA AGAGCCCCTC TCAGGATGAG ATGCTCCCTA CATACCTGAA GGATCTTTCT 
ATTAGCATAG ACAAAGCGGA ACATGAAATC AGCTCTTCTC CTGGGAGTCT GGGAGCCATT 
ATTAACATCC TTGATCTGCT CTCAACAGTT CCAACCCAAG TAAATTCAGA AATGATGACG 
CACGTGCTCT CTACGGTTAA TGTCATCCTT GGCAAGCCCG TCTTGAACAC CTGGAAGGTT 
TTACAACAGC AATGGACCAA TCAGAGTTCA CAGCTACTAC ATTCAGTGGA AAGATTTTCC 
CAAGCATTAC AGTCGGGAGA TAGCCCTCCT TTGTCCTTCT CCCAAACTAA TGTGCAGATG 
AGCAGCATGG TAATCAAGTC CAGCCACCCA GAAACCTATC AACAGAGGTT TGTTTTCCCA 
TACTTTGACC TCTGGGGCAA TGTGGTCATT GACAAGAGCT ATCTAGAAAA CTTGCAGTCG 
GATTCGTCTA TTGTCACCAT GGCTTTCCCA ACTCTCCAAG CCATCCTTGC CCAGGATATC 
CAGGAAAATA ACTTTGCAGA GAGCTTAGTG ATGACAACCA CTGTCAGCCA CAATACAACT 
ATGCCATTCA GGATTTCAAT GACTTTTAAG AACAATAGCC CTTCAGGCGG CGAAACGAAG 
TGTGTCTTCT GGAACTTCAG GCTTGCCAAC AACACAGGGG GGTGGGACAG CAGTGGGTGC 
TATGTAGAAG AAGGTGATGG GGACAATGTC ACCTGTATCT GTGACCACCT AACATCATTC 
TCCATCCTCA TGTCCCCTGA CTCCCCAGAT CCTAGTTCTC TCCTGGGAAT ACTCCTGGAT 
ATTATTTCTT ATGTTGGGGT GGGCTTTTCC ATCTTGAGCT TGGCAGCCTG TCTAGTTGTG 
GAAGCTGTGG TGTGGAAATC GGTGACCAAG AACCGGACTT CTTATATGCG CCACACCTGC 
ATAGTGAATA TCGCTGCCTC CCTTCTGGTC GCCAACACCT GGTTCATTGT GGTCGCTGCC 
ATCCAGGACA ATCGCTACAT ACTCTGCAAG ACAGCCTGTG TGGCTGCCAC CTTCTTCATC 
CACTTCTTCT ACCTCAGCGT CTTCTTCTGG ATGCTGACAC TGGGCCTCAT GCTGTTCTAT 
CGCCTGGTTT TCATTCTGCA TGAAACAAGC AGGTCCACTC AGAAAGCCAT TGCCTTCTGT 
CTTGGCTATG GCTGCCCACT TGCCATCTCG GTCATCACGC TGGGAGCCAC CCAGCCCCGG 
GAAGTCTATA CGAGGAAGAA TGTCTGTTGG CTCAACTGGG AGGACACCAA GGCCCTGCTG 
GCTTTCGCCA TCCCAGCACT GATCATTGTG GTGGTGAACA TAACCATCAC TATTGTGGTC 
ATCACCAAGA TCCTGAGGCC TTCCATTGGA GACAAGCCAT GCAAGCAGGA GAAGAGCAGC 
CTGTTTCAGA TCAGCAAGAG CATTGGGGTC CTCACACCAC TCTTGGGCCT CACTTGGGGT 
TTTGGTCTCA CCACTGTGTT CCCAGGGACC AACCTTGTGT TCCATATCAT ATTTGCCATC 
CTCAATGTCT TCCAGGGATT ATTCATTTTA CTCTTTGGAT GCCTCTGGGA TCTGAAGGTA 
CAGGAAGCTT TGCTGAATAA GTTTTCATTG TCGAGATGGT CTTCACAGCA CTCAAAGTCA 
ACATCCCTGG GTTCATCCAC ACCTGTGTTT TCTATGAGTT CTCCAATATC AAGGAGATTT 
AACAATTTGT TTGGTAAAAC AGCAGCCCCT AAGATCCCCC CAGGCCATGA CGGAAACCTT 
GTTAGCGGTG GCAGTTCAGC AATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865913.1
CDS	832..4338
Translation	MRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVCKLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTAAPKIPPGHDGNLVSGGSSAI

Target ORF information:

RefSeq Version	XM_017010424.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010424.1

ORF Insert Sequence:

ATGAGAGTCA GACTAAATGT AGGCTTTCAA GAAGACCTCA TGAACACTTC CTCCGCCCTC 
TATAGGTCCT ACAAGACCGA CTTGGAAACA GCGTTCCGGA AGGGTTACGG AATTTTACCA 
GGCTTCAAGG GCGTGACTGT GACAGGGTTC AAGTCTGGAA GTGTGGTTGT GACATATGAA 
GTCAAGACTA CACCACCATC ACTTGAGTTA ATACATAAAG CCAATGAACA AGTTGTACAG 
AGCCTCAATC AGACCTACAA AATGGACTAC AACTCCTTTC AAGCAGTTAC TATCAATGAA 
AGCAATTTCT TTGTCACACC AGAAATCATC TTTGAAGGGG ACACAGTCAG TCTGGTGTGT 
GAAAAGGAAG TTTTGTCCTC CAATGTGTCT TGGCGCTATG AAGAACAGCA GTTGGAAATC 
CAGAACAGCA GCAGATTCTC GATTTACACC GCACTTTTCA ACAACATGAC TTCGGTGTCC 
AAGCTCACCA TCCACAACAT CACTCCAGGT GATGCAGGTG AATATGTTTG CAAACTGATA 
TTAGACATTT TTGAATATGA GTGCAAGAAG AAAATAGATG TTATGCCCAT CCAAATTTTG 
GCAAATGAAG AAATGAAGGT GATGTGCGAC AACAATCCTG TATCTTTGAA CTGCTGCAGT 
CAGGGTAATG TTAATTGGAG CAAAGTAGAA TGGAAGCAGG AAGGAAAAAT AAATATTCCA 
GGAACCCCTG AGACAGACAT AGATTCTAGC TGCAGCAGAT ACACCCTCAA GGCTGATGGA 
ACCCAGTGCC CAAGCGGGTC GTCTGGAACA ACAGTCATCT ACACTTGTGA GTTCATCAGT 
GCCTATGGAG CCAGAGGCAG TGCAAACATA AAAGTGACAT TCATCTCTGT GGCCAATCTA 
ACAATAACCC CGGACCCAAT TTCTGTTTCT GAGGGACAAA ACTTTTCTAT AAAATGCATC 
AGTGATGTGA GTAACTATGA TGAGGTTTAT TGGAACACTT CTGCTGGAAT TAAAATATAC 
CAAAGATTTT ATACCACGAG GAGGTATCTT GATGGAGCAG AATCAGTACT GACAGTCAAG 
ACCTCGACCA GGGAGTGGAA TGGAACCTAT CACTGCATAT TTAGATATAA GAATTCATAC 
AGTATTGCAA CCAAAGACGT CATTGTTCAC CCGCTGCCTC TAAAGCTGAA CATCATGGTT 
GATCCTTTGG AAGCTACTGT TTCATGCAGT GGTTCCCATC ACATCAAGTG CTGCATAGAG 
GAGGATGGAG ACTACAAAGT TACTTTCCAT ACGGGTTCCT CATCCCTTCC TGCTGCAAAA 
GAAGTTAACA AAAAACAAGT GTGCTACAAA CACAATTTCA ATGCAAGCTC AGTTTCCTGG 
TGTTCAAAAA CTGTTGATGT GTGTTGTCAC TTTACCAATG CTGCTAATAA TTCAGTCTGG 
AGCCCATCTA TGAAGCTGAA TCTGGTTCCT GGGGAAAACA TCACATGCCA GGATCCCGTA 
ATAGGTGTCG GAGAGCCGGG GAAAGTCATC CAGAAGCTAT GCCGGTTCTC AAACGTTCCC 
AGCAGCCCTG AGAGTCCCAT TGGCGGGACC ATCACTTACA AATGTGTAGG CTCCCAGTGG 
GAGGAGAAGA GAAATGACTG CATCTCTGCC CCAATAAACA GTCTGCTCCA GATGGCTAAG 
GCTTTGATCA AGAGCCCCTC TCAGGATGAG ATGCTCCCTA CATACCTGAA GGATCTTTCT 
ATTAGCATAG ACAAAGCGGA ACATGAAATC AGCTCTTCTC CTGGGAGTCT GGGAGCCATT 
ATTAACATCC TTGATCTGCT CTCAACAGTT CCAACCCAAG TAAATTCAGA AATGATGACG 
CACGTGCTCT CTACGGTTAA TGTCATCCTT GGCAAGCCCG TCTTGAACAC CTGGAAGGTT 
TTACAACAGC AATGGACCAA TCAGAGTTCA CAGCTACTAC ATTCAGTGGA AAGATTTTCC 
CAAGCATTAC AGTCGGGAGA TAGCCCTCCT TTGTCCTTCT CCCAAACTAA TGTGCAGATG 
AGCAGCATGG TAATCAAGTC CAGCCACCCA GAAACCTATC AACAGAGGTT TGTTTTCCCA 
TACTTTGACC TCTGGGGCAA TGTGGTCATT GACAAGAGCT ATCTAGAAAA CTTGCAGTCG 
GATTCGTCTA TTGTCACCAT GGCTTTCCCA ACTCTCCAAG CCATCCTTGC CCAGGATATC 
CAGGAAAATA ACTTTGCAGA GAGCTTAGTG ATGACAACCA CTGTCAGCCA CAATACAACT 
ATGCCATTCA GGATTTCAAT GACTTTTAAG AACAATAGCC CTTCAGGCGG CGAAACGAAG 
TGTGTCTTCT GGAACTTCAG GCTTGCCAAC AACACAGGGG GGTGGGACAG CAGTGGGTGC 
TATGTAGAAG AAGGTGATGG GGACAATGTC ACCTGTATCT GTGACCACCT AACATCATTC 
TCCATCCTCA TGTCCCCTGA CTCCCCAGAT CCTAGTTCTC TCCTGGGAAT ACTCCTGGAT 
ATTATTTCTT ATGTTGGGGT GGGCTTTTCC ATCTTGAGCT TGGCAGCCTG TCTAGTTGTG 
GAAGCTGTGG TGTGGAAATC GGTGACCAAG AACCGGACTT CTTATATGCG CCACACCTGC 
ATAGTGAATA TCGCTGCCTC CCTTCTGGTC GCCAACACCT GGTTCATTGT GGTCGCTGCC 
ATCCAGGACA ATCGCTACAT ACTCTGCAAG ACAGCCTGTG TGGCTGCCAC CTTCTTCATC 
CACTTCTTCT ACCTCAGCGT CTTCTTCTGG ATGCTGACAC TGGGCCTCAT GCTGTTCTAT 
CGCCTGGTTT TCATTCTGCA TGAAACAAGC AGGTCCACTC AGAAAGCCAT TGCCTTCTGT 
CTTGGCTATG GCTGCCCACT TGCCATCTCG GTCATCACGC TGGGAGCCAC CCAGCCCCGG 
GAAGTCTATA CGAGGAAGAA TGTCTGTTGG CTCAACTGGG AGGACACCAA GGCCCTGCTG 
GCTTTCGCCA TCCCAGCACT GATCATTGTG GTGGTGAACA TAACCATCAC TATTGTGGTC 
ATCACCAAGA TCCTGAGGCC TTCCATTGGA GACAAGCCAT GCAAGCAGGA GAAGAGCAGC 
CTGTTTCAGA TCAGCAAGAG CATTGGGGTC CTCACACCAC TCTTGGGCCT CACTTGGGGT 
TTTGGTCTCA CCACTGTGTT CCCAGGGACC AACCTTGTGT TCCATATCAT ATTTGCCATC 
CTCAATGTCT TCCAGGGATT ATTCATTTTA CTCTTTGGAT GCCTCTGGGA TCTGAAGGTA 
CAGGAAGCTT TGCTGAATAA GTTTTCATTG TCGAGATGGT CTTCACAGCA CTCAAAGTCA 
ACATCCCTGG GTTCATCCAC ACCTGTGTTT TCTATGAGTT CTCCAATATC AAGGAGATTT 
AACAATTTGT TTGGTAAAAC AGCAGCCCCT AAGATCCCCC CAGGCCATGA CGGAAACCTT 
GTTAGCGGTG GCAGTTCAGC AATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( XM_017010424.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865913.1 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu96386
Clone ID Related Accession (Same CDS sequence)	XM_017010424.1 , XM_017010424.2
Accession Version	XM_017010424.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017010424.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGTCA GACTAAATGT AGGCTTTCAA GAAGACCTCA TGAACACTTC CTCCGCCCTC 
TATAGGTCCT ACAAGACCGA CTTGGAAACA GCGTTCCGGA AGGGTTACGG AATTTTACCA 
GGCTTCAAGG GCGTGACTGT GACAGGGTTC AAGTCTGGAA GTGTGGTTGT GACATATGAA 
GTCAAGACTA CACCACCATC ACTTGAGTTA ATACATAAAG CCAATGAACA AGTTGTACAG 
AGCCTCAATC AGACCTACAA AATGGACTAC AACTCCTTTC AAGCAGTTAC TATCAATGAA 
AGCAATTTCT TTGTCACACC AGAAATCATC TTTGAAGGGG ACACAGTCAG TCTGGTGTGT 
GAAAAGGAAG TTTTGTCCTC CAATGTGTCT TGGCGCTATG AAGAACAGCA GTTGGAAATC 
CAGAACAGCA GCAGATTCTC GATTTACACC GCACTTTTCA ACAACATGAC TTCGGTGTCC 
AAGCTCACCA TCCACAACAT CACTCCAGGT GATGCAGGTG AATATGTTTG CAAACTGATA 
TTAGACATTT TTGAATATGA GTGCAAGAAG AAAATAGATG TTATGCCCAT CCAAATTTTG 
GCAAATGAAG AAATGAAGGT GATGTGCGAC AACAATCCTG TATCTTTGAA CTGCTGCAGT 
CAGGGTAATG TTAATTGGAG CAAAGTAGAA TGGAAGCAGG AAGGAAAAAT AAATATTCCA 
GGAACCCCTG AGACAGACAT AGATTCTAGC TGCAGCAGAT ACACCCTCAA GGCTGATGGA 
ACCCAGTGCC CAAGCGGGTC GTCTGGAACA ACAGTCATCT ACACTTGTGA GTTCATCAGT 
GCCTATGGAG CCAGAGGCAG TGCAAACATA AAAGTGACAT TCATCTCTGT GGCCAATCTA 
ACAATAACCC CGGACCCAAT TTCTGTTTCT GAGGGACAAA ACTTTTCTAT AAAATGCATC 
AGTGATGTGA GTAACTATGA TGAGGTTTAT TGGAACACTT CTGCTGGAAT TAAAATATAC 
CAAAGATTTT ATACCACGAG GAGGTATCTT GATGGAGCAG AATCAGTACT GACAGTCAAG 
ACCTCGACCA GGGAGTGGAA TGGAACCTAT CACTGCATAT TTAGATATAA GAATTCATAC 
AGTATTGCAA CCAAAGACGT CATTGTTCAC CCGCTGCCTC TAAAGCTGAA CATCATGGTT 
GATCCTTTGG AAGCTACTGT TTCATGCAGT GGTTCCCATC ACATCAAGTG CTGCATAGAG 
GAGGATGGAG ACTACAAAGT TACTTTCCAT ACGGGTTCCT CATCCCTTCC TGCTGCAAAA 
GAAGTTAACA AAAAACAAGT GTGCTACAAA CACAATTTCA ATGCAAGCTC AGTTTCCTGG 
TGTTCAAAAA CTGTTGATGT GTGTTGTCAC TTTACCAATG CTGCTAATAA TTCAGTCTGG 
AGCCCATCTA TGAAGCTGAA TCTGGTTCCT GGGGAAAACA TCACATGCCA GGATCCCGTA 
ATAGGTGTCG GAGAGCCGGG GAAAGTCATC CAGAAGCTAT GCCGGTTCTC AAACGTTCCC 
AGCAGCCCTG AGAGTCCCAT TGGCGGGACC ATCACTTACA AATGTGTAGG CTCCCAGTGG 
GAGGAGAAGA GAAATGACTG CATCTCTGCC CCAATAAACA GTCTGCTCCA GATGGCTAAG 
GCTTTGATCA AGAGCCCCTC TCAGGATGAG ATGCTCCCTA CATACCTGAA GGATCTTTCT 
ATTAGCATAG ACAAAGCGGA ACATGAAATC AGCTCTTCTC CTGGGAGTCT GGGAGCCATT 
ATTAACATCC TTGATCTGCT CTCAACAGTT CCAACCCAAG TAAATTCAGA AATGATGACG 
CACGTGCTCT CTACGGTTAA TGTCATCCTT GGCAAGCCCG TCTTGAACAC CTGGAAGGTT 
TTACAACAGC AATGGACCAA TCAGAGTTCA CAGCTACTAC ATTCAGTGGA AAGATTTTCC 
CAAGCATTAC AGTCGGGAGA TAGCCCTCCT TTGTCCTTCT CCCAAACTAA TGTGCAGATG 
AGCAGCATGG TAATCAAGTC CAGCCACCCA GAAACCTATC AACAGAGGTT TGTTTTCCCA 
TACTTTGACC TCTGGGGCAA TGTGGTCATT GACAAGAGCT ATCTAGAAAA CTTGCAGTCG 
GATTCGTCTA TTGTCACCAT GGCTTTCCCA ACTCTCCAAG CCATCCTTGC CCAGGATATC 
CAGGAAAATA ACTTTGCAGA GAGCTTAGTG ATGACAACCA CTGTCAGCCA CAATACAACT 
ATGCCATTCA GGATTTCAAT GACTTTTAAG AACAATAGCC CTTCAGGCGG CGAAACGAAG 
TGTGTCTTCT GGAACTTCAG GCTTGCCAAC AACACAGGGG GGTGGGACAG CAGTGGGTGC 
TATGTAGAAG AAGGTGATGG GGACAATGTC ACCTGTATCT GTGACCACCT AACATCATTC 
TCCATCCTCA TGTCCCCTGA CTCCCCAGAT CCTAGTTCTC TCCTGGGAAT ACTCCTGGAT 
ATTATTTCTT ATGTTGGGGT GGGCTTTTCC ATCTTGAGCT TGGCAGCCTG TCTAGTTGTG 
GAAGCTGTGG TGTGGAAATC GGTGACCAAG AACCGGACTT CTTATATGCG CCACACCTGC 
ATAGTGAATA TCGCTGCCTC CCTTCTGGTC GCCAACACCT GGTTCATTGT GGTCGCTGCC 
ATCCAGGACA ATCGCTACAT ACTCTGCAAG ACAGCCTGTG TGGCTGCCAC CTTCTTCATC 
CACTTCTTCT ACCTCAGCGT CTTCTTCTGG ATGCTGACAC TGGGCCTCAT GCTGTTCTAT 
CGCCTGGTTT TCATTCTGCA TGAAACAAGC AGGTCCACTC AGAAAGCCAT TGCCTTCTGT 
CTTGGCTATG GCTGCCCACT TGCCATCTCG GTCATCACGC TGGGAGCCAC CCAGCCCCGG 
GAAGTCTATA CGAGGAAGAA TGTCTGTTGG CTCAACTGGG AGGACACCAA GGCCCTGCTG 
GCTTTCGCCA TCCCAGCACT GATCATTGTG GTGGTGAACA TAACCATCAC TATTGTGGTC 
ATCACCAAGA TCCTGAGGCC TTCCATTGGA GACAAGCCAT GCAAGCAGGA GAAGAGCAGC 
CTGTTTCAGA TCAGCAAGAG CATTGGGGTC CTCACACCAC TCTTGGGCCT CACTTGGGGT 
TTTGGTCTCA CCACTGTGTT CCCAGGGACC AACCTTGTGT TCCATATCAT ATTTGCCATC 
CTCAATGTCT TCCAGGGATT ATTCATTTTA CTCTTTGGAT GCCTCTGGGA TCTGAAGGTA 
CAGGAAGCTT TGCTGAATAA GTTTTCATTG TCGAGATGGT CTTCACAGCA CTCAAAGTCA 
ACATCCCTGG GTTCATCCAC ACCTGTGTTT TCTATGAGTT CTCCAATATC AAGGAGATTT 
AACAATTTGT TTGGTAAAAC AGCAGCCCCT AAGATCCCCC CAGGCCATGA CGGAAACCTT 
GTTAGCGGTG GCAGTTCAGC AATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865913.1
CDS	832..4338
Translation	MRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVCKLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTAAPKIPPGHDGNLVSGGSSAI

Target ORF information:

RefSeq Version	XM_017010424.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010424.2

ORF Insert Sequence:

ATGAGAGTCA GACTAAATGT AGGCTTTCAA GAAGACCTCA TGAACACTTC CTCCGCCCTC 
TATAGGTCCT ACAAGACCGA CTTGGAAACA GCGTTCCGGA AGGGTTACGG AATTTTACCA 
GGCTTCAAGG GCGTGACTGT GACAGGGTTC AAGTCTGGAA GTGTGGTTGT GACATATGAA 
GTCAAGACTA CACCACCATC ACTTGAGTTA ATACATAAAG CCAATGAACA AGTTGTACAG 
AGCCTCAATC AGACCTACAA AATGGACTAC AACTCCTTTC AAGCAGTTAC TATCAATGAA 
AGCAATTTCT TTGTCACACC AGAAATCATC TTTGAAGGGG ACACAGTCAG TCTGGTGTGT 
GAAAAGGAAG TTTTGTCCTC CAATGTGTCT TGGCGCTATG AAGAACAGCA GTTGGAAATC 
CAGAACAGCA GCAGATTCTC GATTTACACC GCACTTTTCA ACAACATGAC TTCGGTGTCC 
AAGCTCACCA TCCACAACAT CACTCCAGGT GATGCAGGTG AATATGTTTG CAAACTGATA 
TTAGACATTT TTGAATATGA GTGCAAGAAG AAAATAGATG TTATGCCCAT CCAAATTTTG 
GCAAATGAAG AAATGAAGGT GATGTGCGAC AACAATCCTG TATCTTTGAA CTGCTGCAGT 
CAGGGTAATG TTAATTGGAG CAAAGTAGAA TGGAAGCAGG AAGGAAAAAT AAATATTCCA 
GGAACCCCTG AGACAGACAT AGATTCTAGC TGCAGCAGAT ACACCCTCAA GGCTGATGGA 
ACCCAGTGCC CAAGCGGGTC GTCTGGAACA ACAGTCATCT ACACTTGTGA GTTCATCAGT 
GCCTATGGAG CCAGAGGCAG TGCAAACATA AAAGTGACAT TCATCTCTGT GGCCAATCTA 
ACAATAACCC CGGACCCAAT TTCTGTTTCT GAGGGACAAA ACTTTTCTAT AAAATGCATC 
AGTGATGTGA GTAACTATGA TGAGGTTTAT TGGAACACTT CTGCTGGAAT TAAAATATAC 
CAAAGATTTT ATACCACGAG GAGGTATCTT GATGGAGCAG AATCAGTACT GACAGTCAAG 
ACCTCGACCA GGGAGTGGAA TGGAACCTAT CACTGCATAT TTAGATATAA GAATTCATAC 
AGTATTGCAA CCAAAGACGT CATTGTTCAC CCGCTGCCTC TAAAGCTGAA CATCATGGTT 
GATCCTTTGG AAGCTACTGT TTCATGCAGT GGTTCCCATC ACATCAAGTG CTGCATAGAG 
GAGGATGGAG ACTACAAAGT TACTTTCCAT ACGGGTTCCT CATCCCTTCC TGCTGCAAAA 
GAAGTTAACA AAAAACAAGT GTGCTACAAA CACAATTTCA ATGCAAGCTC AGTTTCCTGG 
TGTTCAAAAA CTGTTGATGT GTGTTGTCAC TTTACCAATG CTGCTAATAA TTCAGTCTGG 
AGCCCATCTA TGAAGCTGAA TCTGGTTCCT GGGGAAAACA TCACATGCCA GGATCCCGTA 
ATAGGTGTCG GAGAGCCGGG GAAAGTCATC CAGAAGCTAT GCCGGTTCTC AAACGTTCCC 
AGCAGCCCTG AGAGTCCCAT TGGCGGGACC ATCACTTACA AATGTGTAGG CTCCCAGTGG 
GAGGAGAAGA GAAATGACTG CATCTCTGCC CCAATAAACA GTCTGCTCCA GATGGCTAAG 
GCTTTGATCA AGAGCCCCTC TCAGGATGAG ATGCTCCCTA CATACCTGAA GGATCTTTCT 
ATTAGCATAG ACAAAGCGGA ACATGAAATC AGCTCTTCTC CTGGGAGTCT GGGAGCCATT 
ATTAACATCC TTGATCTGCT CTCAACAGTT CCAACCCAAG TAAATTCAGA AATGATGACG 
CACGTGCTCT CTACGGTTAA TGTCATCCTT GGCAAGCCCG TCTTGAACAC CTGGAAGGTT 
TTACAACAGC AATGGACCAA TCAGAGTTCA CAGCTACTAC ATTCAGTGGA AAGATTTTCC 
CAAGCATTAC AGTCGGGAGA TAGCCCTCCT TTGTCCTTCT CCCAAACTAA TGTGCAGATG 
AGCAGCATGG TAATCAAGTC CAGCCACCCA GAAACCTATC AACAGAGGTT TGTTTTCCCA 
TACTTTGACC TCTGGGGCAA TGTGGTCATT GACAAGAGCT ATCTAGAAAA CTTGCAGTCG 
GATTCGTCTA TTGTCACCAT GGCTTTCCCA ACTCTCCAAG CCATCCTTGC CCAGGATATC 
CAGGAAAATA ACTTTGCAGA GAGCTTAGTG ATGACAACCA CTGTCAGCCA CAATACAACT 
ATGCCATTCA GGATTTCAAT GACTTTTAAG AACAATAGCC CTTCAGGCGG CGAAACGAAG 
TGTGTCTTCT GGAACTTCAG GCTTGCCAAC AACACAGGGG GGTGGGACAG CAGTGGGTGC 
TATGTAGAAG AAGGTGATGG GGACAATGTC ACCTGTATCT GTGACCACCT AACATCATTC 
TCCATCCTCA TGTCCCCTGA CTCCCCAGAT CCTAGTTCTC TCCTGGGAAT ACTCCTGGAT 
ATTATTTCTT ATGTTGGGGT GGGCTTTTCC ATCTTGAGCT TGGCAGCCTG TCTAGTTGTG 
GAAGCTGTGG TGTGGAAATC GGTGACCAAG AACCGGACTT CTTATATGCG CCACACCTGC 
ATAGTGAATA TCGCTGCCTC CCTTCTGGTC GCCAACACCT GGTTCATTGT GGTCGCTGCC 
ATCCAGGACA ATCGCTACAT ACTCTGCAAG ACAGCCTGTG TGGCTGCCAC CTTCTTCATC 
CACTTCTTCT ACCTCAGCGT CTTCTTCTGG ATGCTGACAC TGGGCCTCAT GCTGTTCTAT 
CGCCTGGTTT TCATTCTGCA TGAAACAAGC AGGTCCACTC AGAAAGCCAT TGCCTTCTGT 
CTTGGCTATG GCTGCCCACT TGCCATCTCG GTCATCACGC TGGGAGCCAC CCAGCCCCGG 
GAAGTCTATA CGAGGAAGAA TGTCTGTTGG CTCAACTGGG AGGACACCAA GGCCCTGCTG 
GCTTTCGCCA TCCCAGCACT GATCATTGTG GTGGTGAACA TAACCATCAC TATTGTGGTC 
ATCACCAAGA TCCTGAGGCC TTCCATTGGA GACAAGCCAT GCAAGCAGGA GAAGAGCAGC 
CTGTTTCAGA TCAGCAAGAG CATTGGGGTC CTCACACCAC TCTTGGGCCT CACTTGGGGT 
TTTGGTCTCA CCACTGTGTT CCCAGGGACC AACCTTGTGT TCCATATCAT ATTTGCCATC 
CTCAATGTCT TCCAGGGATT ATTCATTTTA CTCTTTGGAT GCCTCTGGGA TCTGAAGGTA 
CAGGAAGCTT TGCTGAATAA GTTTTCATTG TCGAGATGGT CTTCACAGCA CTCAAAGTCA 
ACATCCCTGG GTTCATCCAC ACCTGTGTTT TCTATGAGTT CTCCAATATC AAGGAGATTT 
AACAATTTGT TTGGTAAAAC AGCAGCCCCT AAGATCCCCC CAGGCCATGA CGGAAACCTT 
GTTAGCGGTG GCAGTTCAGC AATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( XM_011514354.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512656.1 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X11, mRNA.

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CloneID	OHu78035
Clone ID Related Accession (Same CDS sequence)	XM_011514354.2
Accession Version	XM_011514354.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514354.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGTCA GACTAAATGT AGGCTTTCAA GAAGACCTCA TGAACACTTC CTCCGCCCTC 
TATAGGTCCT ACAAGACCGA CTTGGAAACA GCGTTCCGGA AGGGTTACGG AATTTTACCA 
GGCTTCAAGG GCGTGACTGT GACAGGGTTC AAGTCTGGAA GTGTGGTTGT GACATATGAA 
GTCAAGACTA CACCACCATC ACTTGAGTTA ATACATAAAG CCAATGAACA AGTTGTACAG 
AGCCTCAATC AGACCTACAA AATGGACTAC AACTCCTTTC AAGCAGTTAC TATCAATGAA 
AGCAATTTCT TTGTCACACC AGAAATCATC TTTGAAGGGG ACACAGTCAG TCTGGTGTGT 
GAAAAGGAAG TTTTGTCCTC CAATGTGTCT TGGCGCTATG AAGAACAGCA GTTGGAAATC 
CAGAACAGCA GCAGATTCTC GATTTACACC GCACTTTTCA ACAACATGAC TTCGGTGTCC 
AAGCTCACCA TCCACAACAT CACTCCAGGT GATGCAGGTG AATATGTTTG CAAACTGATA 
TTAGACATTT TTGAATATGA GTGCAAGAAG AAAATAGATG TTATGCCCAT CCAAATTTTG 
GCAAATGAAG AAATGAAGGT GATGTGCGAC AACAATCCTG TATCTTTGAA CTGCTGCAGT 
CAGGGTAATG TTAATTGGAG CAAAGTAGAA TGGAAGCAGG AAGGAAAAAT AAATATTCCA 
GGAACCCCTG AGACAGACAT AGATTCTAGC TGCAGCAGAT ACACCCTCAA GGCTGATGGA 
ACCCAGTGCC CAAGCGGGTC GTCTGGAACA ACAGTCATCT ACACTTGTGA GTTCATCAGT 
GCCTATGGAG CCAGAGGCAG TGCAAACATA AAAGTGACAT TCATCTCTGT GGGAGATAAT 
TCCAGCCACA AAAGTGAAGT GCACGAGGTC CTAAGGCATG TGATGGAAAT GAGTACAGTG 
CAGAGCACCA CCAGGAACAG CCTAGAAACG AGTAAAGCCC AGGAGCAGCA AGCCAATCTA 
ACAATAACCC CGGACCCAAT TTCTGTTTCT GAGGGACAAA ACTTTTCTAT AAAATGCATC 
AGTGATGTGA GTAACTATGA TGAGGTTTAT TGGAACACTT CTGCTGGAAT TAAAATATAC 
CAAAGATTTT ATACCACGAG GAGGTATCTT GATGGAGCAG AATCAGTACT GACAGTCAAG 
ACCTCGACCA GGGAGTGGAA TGGAACCTAT CACTGCATAT TTAGATATAA GAATTCATAC 
AGTATTGCAA CCAAAGACGT CATTGTTCAC CCGCTGCCTC TAAAGCTGAA CATCATGGTT 
GATCCTTTGG AAGCTACTGT TTCATGCAGT GGTTCCCATC ACATCAAGTG CTGCATAGAG 
GAGGATGGAG ACTACAAAGT TACTTTCCAT ACGGGTTCCT CATCCCTTCC TGCTGCAAAA 
GAAGTTAACA AAAAACAAGT GTGCTACAAA CACAATTTCA ATGCAAGCTC AGTTTCCTGG 
TGTTCAAAAA CTGTTGATGT GTGTTGTCAC TTTACCAATG CTGCTAATAA TTCAGTCTGG 
AGCCCATCTA TGAAGCTGAA TCTGGTTCCT GGGGAAAACA TCACATGCCA GGATCCCGTA 
ATAGGTGTCG GAGAGCCGGG GAAAGTCATC CAGAAGCTAT GCCGGTTCTC AAACGTTCCC 
AGCAGCCCTG AGAGTCCCAT TGGCGGGACC ATCACTTACA AATGTGTAGG CTCCCAGTGG 
GAGGAGAAGA GAAATGACTG CATCTCTGCC CCAATAAACA GTCTGCTCCA GATGGCTAAG 
GCTTTGATCA AGAGCCCCTC TCAGGATGAG ATGCTCCCTA CATACCTGAA GGATCTTTCT 
ATTAGCATAG ACAAAGCGGA ACATGAAATC AGCTCTTCTC CTGGGAGTCT GGGAGCCATT 
ATTAACATCC TTGATCTGCT CTCAACAGTT CCAACCCAAG TAAATTCAGA AATGATGACG 
CACGTGCTCT CTACGGTTAA TGTCATCCTT GGCAAGCCCG TCTTGAACAC CTGGAAGGTT 
TTACAACAGC AATGGACCAA TCAGAGTTCA CAGCTACTAC ATTCAGTGGA AAGATTTTCC 
CAAGCATTAC AGTCGGGAGA TAGCCCTCCT TTGTCCTTCT CCCAAACTAA TGTGCAGATG 
AGCAGCATGG TAATCAAGTC CAGCCACCCA GAAACCTATC AACAGAGGTT TGTTTTCCCA 
TACTTTGACC TCTGGGGCAA TGTGGTCATT GACAAGAGCT ATCTAGAAAA CTTGCAGTCG 
GATTCGTCTA TTGTCACCAT GGCTTTCCCA ACTCTCCAAG CCATCCTTGC CCAGGATATC 
CAGGAAAATA ACTTTGCAGA GAGCTTAGTG ATGACAACCA CTGTCAGCCA CAATACAACT 
ATGCCATTCA GGATTTCAAT GACTTTTAAG AACAATAGCC CTTCAGGCGG CGAAACGAAG 
TGTGTCTTCT GGAACTTCAG GCTTGCCAAC AACACAGGGG GGTGGGACAG CAGTGGGTGC 
TATGTAGAAG AAGGTGATGG GGACAATGTC ACCTGTATCT GTGACCACCT AACATCATTC 
TCCATCCTCA TGTCCCCTGA CTCCCCAGAT CCTAGTTCTC TCCTGGGAAT ACTCCTGGAT 
ATTATTTCTT ATGTTGGGGT GGGCTTTTCC ATCTTGAGCT TGGCAGCCTG TCTAGTTGTG 
GAAGCTGTGG TGTGGAAATC GGTGACCAAG AACCGGACTT CTTATATGCG CCACACCTGC 
ATAGTGAATA TCGCTGCCTC CCTTCTGGTC GCCAACACCT GGTTCATTGT GGTCGCTGCC 
ATCCAGGACA ATCGCTACAT ACTCTGCAAG ACAGCCTGTG TGGCTGCCAC CTTCTTCATC 
CACTTCTTCT ACCTCAGCGT CTTCTTCTGG ATGCTGACAC TGGGCCTCAT GCTGTTCTAT 
CGCCTGGTTT TCATTCTGCA TGAAACAAGC AGGTCCACTC AGAAAGCCAT TGCCTTCTGT 
CTTGGCTATG GCTGCCCACT TGCCATCTCG GTCATCACGC TGGGAGCCAC CCAGCCCCGG 
GAAGTCTATA CGAGGAAGAA TGTCTGTTGG CTCAACTGGG AGGACACCAA GGCCCTGCTG 
GCTTTCGCCA TCCCAGCACT GATCATTGTG GTGGTGAACA TAACCATCAC TATTGTGGTC 
ATCACCAAGA TCCTGAGGCC TTCCATTGGA GACAAGCCAT GCAAGCAGGA GAAGAGCAGC 
CTGTTTCAGA TCAGCAAGAG CATTGGGGTC CTCACACCAC TCTTGGGCCT CACTTGGGGT 
TTTGGTCTCA CCACTGTGTT CCCAGGGACC AACCTTGTGT TCCATATCAT ATTTGCCATC 
CTCAATGTCT TCCAGGGATT ATTCATTTTA CTCTTTGGAT GCCTCTGGGA TCTGAAGGTA 
CAGGAAGCTT TGCTGAATAA GTTTTCATTG TCGAGATGGT CTTCACAGCA CTCAAAGTCA 
ACATCCCTGG GTTCATCCAC ACCTGTGTTT TCTATGAGTT CTCCAATATC AAGGAGATTT 
AACAATTTGT TTGGTAAAAC AGGAACGTAT AATGTTTCCA CCCCAGAAGC AACCAGCTCA 
TCCCTGGAAA ACTCATCCAG TGCTTCTTCG TTGCTCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512656.1
CDS	832..4473
Translation	MRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVCKLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVGDNSSHKSEVHEVLRHVMEMSTVQSTTRNSLETSKAQEQQANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPEATSSSLENSSSASSLLN

Target ORF information:

RefSeq Version	XM_011514354.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514354.2

ORF Insert Sequence:

ATGAGAGTCA GACTAAATGT AGGCTTTCAA GAAGACCTCA TGAACACTTC CTCCGCCCTC 
TATAGGTCCT ACAAGACCGA CTTGGAAACA GCGTTCCGGA AGGGTTACGG AATTTTACCA 
GGCTTCAAGG GCGTGACTGT GACAGGGTTC AAGTCTGGAA GTGTGGTTGT GACATATGAA 
GTCAAGACTA CACCACCATC ACTTGAGTTA ATACATAAAG CCAATGAACA AGTTGTACAG 
AGCCTCAATC AGACCTACAA AATGGACTAC AACTCCTTTC AAGCAGTTAC TATCAATGAA 
AGCAATTTCT TTGTCACACC AGAAATCATC TTTGAAGGGG ACACAGTCAG TCTGGTGTGT 
GAAAAGGAAG TTTTGTCCTC CAATGTGTCT TGGCGCTATG AAGAACAGCA GTTGGAAATC 
CAGAACAGCA GCAGATTCTC GATTTACACC GCACTTTTCA ACAACATGAC TTCGGTGTCC 
AAGCTCACCA TCCACAACAT CACTCCAGGT GATGCAGGTG AATATGTTTG CAAACTGATA 
TTAGACATTT TTGAATATGA GTGCAAGAAG AAAATAGATG TTATGCCCAT CCAAATTTTG 
GCAAATGAAG AAATGAAGGT GATGTGCGAC AACAATCCTG TATCTTTGAA CTGCTGCAGT 
CAGGGTAATG TTAATTGGAG CAAAGTAGAA TGGAAGCAGG AAGGAAAAAT AAATATTCCA 
GGAACCCCTG AGACAGACAT AGATTCTAGC TGCAGCAGAT ACACCCTCAA GGCTGATGGA 
ACCCAGTGCC CAAGCGGGTC GTCTGGAACA ACAGTCATCT ACACTTGTGA GTTCATCAGT 
GCCTATGGAG CCAGAGGCAG TGCAAACATA AAAGTGACAT TCATCTCTGT GGGAGATAAT 
TCCAGCCACA AAAGTGAAGT GCACGAGGTC CTAAGGCATG TGATGGAAAT GAGTACAGTG 
CAGAGCACCA CCAGGAACAG CCTAGAAACG AGTAAAGCCC AGGAGCAGCA AGCCAATCTA 
ACAATAACCC CGGACCCAAT TTCTGTTTCT GAGGGACAAA ACTTTTCTAT AAAATGCATC 
AGTGATGTGA GTAACTATGA TGAGGTTTAT TGGAACACTT CTGCTGGAAT TAAAATATAC 
CAAAGATTTT ATACCACGAG GAGGTATCTT GATGGAGCAG AATCAGTACT GACAGTCAAG 
ACCTCGACCA GGGAGTGGAA TGGAACCTAT CACTGCATAT TTAGATATAA GAATTCATAC 
AGTATTGCAA CCAAAGACGT CATTGTTCAC CCGCTGCCTC TAAAGCTGAA CATCATGGTT 
GATCCTTTGG AAGCTACTGT TTCATGCAGT GGTTCCCATC ACATCAAGTG CTGCATAGAG 
GAGGATGGAG ACTACAAAGT TACTTTCCAT ACGGGTTCCT CATCCCTTCC TGCTGCAAAA 
GAAGTTAACA AAAAACAAGT GTGCTACAAA CACAATTTCA ATGCAAGCTC AGTTTCCTGG 
TGTTCAAAAA CTGTTGATGT GTGTTGTCAC TTTACCAATG CTGCTAATAA TTCAGTCTGG 
AGCCCATCTA TGAAGCTGAA TCTGGTTCCT GGGGAAAACA TCACATGCCA GGATCCCGTA 
ATAGGTGTCG GAGAGCCGGG GAAAGTCATC CAGAAGCTAT GCCGGTTCTC AAACGTTCCC 
AGCAGCCCTG AGAGTCCCAT TGGCGGGACC ATCACTTACA AATGTGTAGG CTCCCAGTGG 
GAGGAGAAGA GAAATGACTG CATCTCTGCC CCAATAAACA GTCTGCTCCA GATGGCTAAG 
GCTTTGATCA AGAGCCCCTC TCAGGATGAG ATGCTCCCTA CATACCTGAA GGATCTTTCT 
ATTAGCATAG ACAAAGCGGA ACATGAAATC AGCTCTTCTC CTGGGAGTCT GGGAGCCATT 
ATTAACATCC TTGATCTGCT CTCAACAGTT CCAACCCAAG TAAATTCAGA AATGATGACG 
CACGTGCTCT CTACGGTTAA TGTCATCCTT GGCAAGCCCG TCTTGAACAC CTGGAAGGTT 
TTACAACAGC AATGGACCAA TCAGAGTTCA CAGCTACTAC ATTCAGTGGA AAGATTTTCC 
CAAGCATTAC AGTCGGGAGA TAGCCCTCCT TTGTCCTTCT CCCAAACTAA TGTGCAGATG 
AGCAGCATGG TAATCAAGTC CAGCCACCCA GAAACCTATC AACAGAGGTT TGTTTTCCCA 
TACTTTGACC TCTGGGGCAA TGTGGTCATT GACAAGAGCT ATCTAGAAAA CTTGCAGTCG 
GATTCGTCTA TTGTCACCAT GGCTTTCCCA ACTCTCCAAG CCATCCTTGC CCAGGATATC 
CAGGAAAATA ACTTTGCAGA GAGCTTAGTG ATGACAACCA CTGTCAGCCA CAATACAACT 
ATGCCATTCA GGATTTCAAT GACTTTTAAG AACAATAGCC CTTCAGGCGG CGAAACGAAG 
TGTGTCTTCT GGAACTTCAG GCTTGCCAAC AACACAGGGG GGTGGGACAG CAGTGGGTGC 
TATGTAGAAG AAGGTGATGG GGACAATGTC ACCTGTATCT GTGACCACCT AACATCATTC 
TCCATCCTCA TGTCCCCTGA CTCCCCAGAT CCTAGTTCTC TCCTGGGAAT ACTCCTGGAT 
ATTATTTCTT ATGTTGGGGT GGGCTTTTCC ATCTTGAGCT TGGCAGCCTG TCTAGTTGTG 
GAAGCTGTGG TGTGGAAATC GGTGACCAAG AACCGGACTT CTTATATGCG CCACACCTGC 
ATAGTGAATA TCGCTGCCTC CCTTCTGGTC GCCAACACCT GGTTCATTGT GGTCGCTGCC 
ATCCAGGACA ATCGCTACAT ACTCTGCAAG ACAGCCTGTG TGGCTGCCAC CTTCTTCATC 
CACTTCTTCT ACCTCAGCGT CTTCTTCTGG ATGCTGACAC TGGGCCTCAT GCTGTTCTAT 
CGCCTGGTTT TCATTCTGCA TGAAACAAGC AGGTCCACTC AGAAAGCCAT TGCCTTCTGT 
CTTGGCTATG GCTGCCCACT TGCCATCTCG GTCATCACGC TGGGAGCCAC CCAGCCCCGG 
GAAGTCTATA CGAGGAAGAA TGTCTGTTGG CTCAACTGGG AGGACACCAA GGCCCTGCTG 
GCTTTCGCCA TCCCAGCACT GATCATTGTG GTGGTGAACA TAACCATCAC TATTGTGGTC 
ATCACCAAGA TCCTGAGGCC TTCCATTGGA GACAAGCCAT GCAAGCAGGA GAAGAGCAGC 
CTGTTTCAGA TCAGCAAGAG CATTGGGGTC CTCACACCAC TCTTGGGCCT CACTTGGGGT 
TTTGGTCTCA CCACTGTGTT CCCAGGGACC AACCTTGTGT TCCATATCAT ATTTGCCATC 
CTCAATGTCT TCCAGGGATT ATTCATTTTA CTCTTTGGAT GCCTCTGGGA TCTGAAGGTA 
CAGGAAGCTT TGCTGAATAA GTTTTCATTG TCGAGATGGT CTTCACAGCA CTCAAAGTCA 
ACATCCCTGG GTTCATCCAC ACCTGTGTTT TCTATGAGTT CTCCAATATC AAGGAGATTT 
AACAATTTGT TTGGTAAAAC AGGAACGTAT AATGTTTCCA CCCCAGAAGC AACCAGCTCA 
TCCCTGGAAA ACTCATCCAG TGCTTCTTCG TTGCTCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( NM_001098518.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001091988.1 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant 2, mRNA.

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CloneID	OHu23485
Clone ID Related Accession (Same CDS sequence)	NM_001098518.2 , NM_015234.4 , NM_015234.5 , NM_001098518.1
Accession Version	NM_001098518.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB230925.1, AB018301.2, AL096772.5, AY498875.1 and AY140958.1. On Nov 23, 2018 this sequence version replaced NM_001098518.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.261933.1, SRR1660807.130135.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000283296.12/ ENSP00000283296.7 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GGAACGTATA ATGTTTCCAC CCCAGAAGCA ACCAGCTCAT CCCTGGAAAA CTCATCCAGT 
GCTTCTTCGT TGCTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091988.1
CDS	251..4291
Translation	MKSPRRTTLCLMFIVIYSSKAALNWNYESTIHPLSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEISFENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVCKLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPEATSSSLENSSSASSLLN

Target ORF information:

RefSeq Version	NM_001098518.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098518.2

ORF Insert Sequence:

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GGAACGTATA ATGTTTCCAC CCCAGAAGCA ACCAGCTCAT CCCTGGAAAA CTCATCCAGT 
GCTTCTTCGT TGCTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( NM_015234.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056049.4 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23485
Clone ID Related Accession (Same CDS sequence)	NM_001098518.2 , NM_015234.4 , NM_015234.5 , NM_001098518.1
Accession Version	NM_015234.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA588699.1, AY498875.1, AL096772.5, AY140958.1 and AI796309.1. On Jun 7, 2007 this sequence version replaced NM_015234.3. Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.71555.1, SRR1803613.23656.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GGAACGTATA ATGTTTCCAC CCCAGAAGCA ACCAGCTCAT CCCTGGAAAA CTCATCCAGT 
GCTTCTTCGT TGCTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056049.4
CDS	230..4270
Translation	MKSPRRTTLCLMFIVIYSSKAALNWNYESTIHPLSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEISFENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVCKLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPEATSSSLENSSSASSLLN

Target ORF information:

RefSeq Version	NM_015234.4
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015234.4

ORF Insert Sequence:

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GGAACGTATA ATGTTTCCAC CCCAGAAGCA ACCAGCTCAT CCCTGGAAAA CTCATCCAGT 
GCTTCTTCGT TGCTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( NM_015234.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_056049.4 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant 1, mRNA.

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CloneID	OHu23485
Clone ID Related Accession (Same CDS sequence)	NM_001098518.2 , NM_015234.4 , NM_015234.5 , NM_001098518.1
Accession Version	NM_015234.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL096772.5, DA588699.1, AY498875.1 and AY140958.1. On May 31, 2019 this sequence version replaced NM_015234.4. Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.71555.1, SRR1803613.23656.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GGAACGTATA ATGTTTCCAC CCCAGAAGCA ACCAGCTCAT CCCTGGAAAA CTCATCCAGT 
GCTTCTTCGT TGCTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056049.4
CDS	231..4271
Translation	MKSPRRTTLCLMFIVIYSSKAALNWNYESTIHPLSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEISFENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVCKLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPEATSSSLENSSSASSLLN

Target ORF information:

RefSeq Version	NM_015234.5
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015234.5

ORF Insert Sequence:

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GGAACGTATA ATGTTTCCAC CCCAGAAGCA ACCAGCTCAT CCCTGGAAAA CTCATCCAGT 
GCTTCTTCGT TGCTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( NM_001098518.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001091988.1 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant 2, mRNA.

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CloneID	OHu23485
Clone ID Related Accession (Same CDS sequence)	NM_001098518.2 , NM_015234.4 , NM_015234.5 , NM_001098518.1
Accession Version	NM_001098518.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB230925.1, AB018301.2, AL096772.5, AY498875.1, AY140958.1 and AI796309.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.261933.1, SRR1660807.130135.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GGAACGTATA ATGTTTCCAC CCCAGAAGCA ACCAGCTCAT CCCTGGAAAA CTCATCCAGT 
GCTTCTTCGT TGCTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091988.1
CDS	290..4330
Translation	MKSPRRTTLCLMFIVIYSSKAALNWNYESTIHPLSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEISFENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVCKLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPEATSSSLENSSSASSLLN

Target ORF information:

RefSeq Version	NM_001098518.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098518.1

ORF Insert Sequence:

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GGAACGTATA ATGTTTCCAC CCCAGAAGCA ACCAGCTCAT CCCTGGAAAA CTCATCCAGT 
GCTTCTTCGT TGCTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( XM_005248892.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248949.1 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu40185
Clone ID Related Accession (Same CDS sequence)	XM_005248892.3 , XM_005248893.3 , XM_005248892.2
Accession Version	XM_005248892.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005248892.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GGAGATAATT CCAGCCACAA AAGTGAAGTG 
CACGAGGTCC TAAGGCATGT GATGGAAATG AGTACAGTGC AGAGCACCAC CAGGAACAGC 
CTAGAAACGA GTAAAGCCCA GGAGCAGCAA GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GGAACGTATA ATGTTTCCAC CCCAGAAGCA ACCAGCTCAT CCCTGGAAAA CTCATCCAGT 
GCTTCTTCGT TGCTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248949.1
CDS	277..4437
Translation	MKSPRRTTLCLMFIVIYSSKAALNWNYESTIHPLSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEISFENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVCKLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVGDNSSHKSEVHEVLRHVMEMSTVQSTTRNSLETSKAQEQQANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPEATSSSLENSSSASSLLN

Target ORF information:

RefSeq Version	XM_005248892.3
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248892.3

ORF Insert Sequence:

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GGAGATAATT CCAGCCACAA AAGTGAAGTG 
CACGAGGTCC TAAGGCATGT GATGGAAATG AGTACAGTGC AGAGCACCAC CAGGAACAGC 
CTAGAAACGA GTAAAGCCCA GGAGCAGCAA GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GGAACGTATA ATGTTTCCAC CCCAGAAGCA ACCAGCTCAT CCCTGGAAAA CTCATCCAGT 
GCTTCTTCGT TGCTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( XM_005248893.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248950.1 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu40185
Clone ID Related Accession (Same CDS sequence)	XM_005248892.3 , XM_005248893.3 , XM_005248892.2
Accession Version	XM_005248893.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005248893.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GGAGATAATT CCAGCCACAA AAGTGAAGTG 
CACGAGGTCC TAAGGCATGT GATGGAAATG AGTACAGTGC AGAGCACCAC CAGGAACAGC 
CTAGAAACGA GTAAAGCCCA GGAGCAGCAA GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GGAACGTATA ATGTTTCCAC CCCAGAAGCA ACCAGCTCAT CCCTGGAAAA CTCATCCAGT 
GCTTCTTCGT TGCTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248950.1
CDS	246..4406
Translation	MKSPRRTTLCLMFIVIYSSKAALNWNYESTIHPLSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEISFENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVCKLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVGDNSSHKSEVHEVLRHVMEMSTVQSTTRNSLETSKAQEQQANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPEATSSSLENSSSASSLLN

Target ORF information:

RefSeq Version	XM_005248893.3
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248893.3

ORF Insert Sequence:

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GGAGATAATT CCAGCCACAA AAGTGAAGTG 
CACGAGGTCC TAAGGCATGT GATGGAAATG AGTACAGTGC AGAGCACCAC CAGGAACAGC 
CTAGAAACGA GTAAAGCCCA GGAGCAGCAA GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GGAACGTATA ATGTTTCCAC CCCAGAAGCA ACCAGCTCAT CCCTGGAAAA CTCATCCAGT 
GCTTCTTCGT TGCTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( XM_005248892.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248949.1 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X1, mRNA.

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CloneID	OHu40185
Clone ID Related Accession (Same CDS sequence)	XM_005248892.3 , XM_005248893.3 , XM_005248892.2
Accession Version	XM_005248892.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005248892.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GGAGATAATT CCAGCCACAA AAGTGAAGTG 
CACGAGGTCC TAAGGCATGT GATGGAAATG AGTACAGTGC AGAGCACCAC CAGGAACAGC 
CTAGAAACGA GTAAAGCCCA GGAGCAGCAA GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GGAACGTATA ATGTTTCCAC CCCAGAAGCA ACCAGCTCAT CCCTGGAAAA CTCATCCAGT 
GCTTCTTCGT TGCTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248949.1
CDS	290..4450
Translation	MKSPRRTTLCLMFIVIYSSKAALNWNYESTIHPLSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEISFENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVCKLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVGDNSSHKSEVHEVLRHVMEMSTVQSTTRNSLETSKAQEQQANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPEATSSSLENSSSASSLLN

Target ORF information:

RefSeq Version	XM_005248892.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248892.2

ORF Insert Sequence:

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GGAGATAATT CCAGCCACAA AAGTGAAGTG 
CACGAGGTCC TAAGGCATGT GATGGAAATG AGTACAGTGC AGAGCACCAC CAGGAACAGC 
CTAGAAACGA GTAAAGCCCA GGAGCAGCAA GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GGAACGTATA ATGTTTCCAC CCCAGAAGCA ACCAGCTCAT CCCTGGAAAA CTCATCCAGT 
GCTTCTTCGT TGCTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( XM_005248894.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248951.1 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu40186
Clone ID Related Accession (Same CDS sequence)	XM_005248894.4 , XM_005248894.3
Accession Version	XM_005248894.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005248894.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GGAGATAATT CCAGCCACAA AAGTGAAGTG 
CACGAGGTCC TAAGGCATGT GATGGAAATG AGTACAGTGC AGAGCACCAC CAGGAACAGC 
CTAGAAACGA GTAAAGCCCA GGAGCAGCAA GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCAGCCCCTA AGATCCCCCC AGGCCATGAC GGAAACCTTG TTAGCGGTGG CAGTTCAGCA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248951.1
CDS	277..4422
Translation	MKSPRRTTLCLMFIVIYSSKAALNWNYESTIHPLSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEISFENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVCKLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVGDNSSHKSEVHEVLRHVMEMSTVQSTTRNSLETSKAQEQQANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTAAPKIPPGHDGNLVSGGSSAI

Target ORF information:

RefSeq Version	XM_005248894.4
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248894.4

ORF Insert Sequence:

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GGAGATAATT CCAGCCACAA AAGTGAAGTG 
CACGAGGTCC TAAGGCATGT GATGGAAATG AGTACAGTGC AGAGCACCAC CAGGAACAGC 
CTAGAAACGA GTAAAGCCCA GGAGCAGCAA GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCAGCCCCTA AGATCCCCCC AGGCCATGAC GGAAACCTTG TTAGCGGTGG CAGTTCAGCA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( XM_005248894.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248951.1 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu40186
Clone ID Related Accession (Same CDS sequence)	XM_005248894.4 , XM_005248894.3
Accession Version	XM_005248894.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005248894.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GGAGATAATT CCAGCCACAA AAGTGAAGTG 
CACGAGGTCC TAAGGCATGT GATGGAAATG AGTACAGTGC AGAGCACCAC CAGGAACAGC 
CTAGAAACGA GTAAAGCCCA GGAGCAGCAA GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCAGCCCCTA AGATCCCCCC AGGCCATGAC GGAAACCTTG TTAGCGGTGG CAGTTCAGCA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248951.1
CDS	290..4435
Translation	MKSPRRTTLCLMFIVIYSSKAALNWNYESTIHPLSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEISFENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVCKLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVGDNSSHKSEVHEVLRHVMEMSTVQSTTRNSLETSKAQEQQANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTAAPKIPPGHDGNLVSGGSSAI

Target ORF information:

RefSeq Version	XM_005248894.3
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248894.3

ORF Insert Sequence:

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GGAGATAATT CCAGCCACAA AAGTGAAGTG 
CACGAGGTCC TAAGGCATGT GATGGAAATG AGTACAGTGC AGAGCACCAC CAGGAACAGC 
CTAGAAACGA GTAAAGCCCA GGAGCAGCAA GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCAGCCCCTA AGATCCCCCC AGGCCATGAC GGAAACCTTG TTAGCGGTGG CAGTTCAGCA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( XM_005248895.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248952.1 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X4, mRNA.

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CloneID	OHu40187
Clone ID Related Accession (Same CDS sequence)	XM_005248895.3 , XM_017010418.2 , XM_017010418.1 , XM_005248895.4
Accession Version	XM_005248895.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005248895.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GGAGATAATT CCAGCCACAA AAGTGAAGTG 
CACGAGGTCC TAAGGCATGT GATGGAAATG AGTACAGTGC AGAGCACCAC CAGGAACAGC 
CTAGAAACGA GTAAAGCCCA GGAGCAGCAA GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCCCCTAAGA TCCCCCCAGG CCATGACGGA AACCTTGTTA GCGGTGGCAG TTCAGCAATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248952.1
CDS	290..4432
Translation	MKSPRRTTLCLMFIVIYSSKAALNWNYESTIHPLSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEISFENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVCKLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVGDNSSHKSEVHEVLRHVMEMSTVQSTTRNSLETSKAQEQQANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTAPKIPPGHDGNLVSGGSSAI

Target ORF information:

RefSeq Version	XM_005248895.3
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248895.3

ORF Insert Sequence:

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GGAGATAATT CCAGCCACAA AAGTGAAGTG 
CACGAGGTCC TAAGGCATGT GATGGAAATG AGTACAGTGC AGAGCACCAC CAGGAACAGC 
CTAGAAACGA GTAAAGCCCA GGAGCAGCAA GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCCCCTAAGA TCCCCCCAGG CCATGACGGA AACCTTGTTA GCGGTGGCAG TTCAGCAATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( XM_017010418.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865907.1 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X5, mRNA.

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CloneID	OHu40187
Clone ID Related Accession (Same CDS sequence)	XM_005248895.3 , XM_017010418.2 , XM_017010418.1 , XM_005248895.4
Accession Version	XM_017010418.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017010418.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GGAGATAATT CCAGCCACAA AAGTGAAGTG 
CACGAGGTCC TAAGGCATGT GATGGAAATG AGTACAGTGC AGAGCACCAC CAGGAACAGC 
CTAGAAACGA GTAAAGCCCA GGAGCAGCAA GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCCCCTAAGA TCCCCCCAGG CCATGACGGA AACCTTGTTA GCGGTGGCAG TTCAGCAATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865907.1
CDS	246..4388
Translation	MKSPRRTTLCLMFIVIYSSKAALNWNYESTIHPLSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEISFENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVCKLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVGDNSSHKSEVHEVLRHVMEMSTVQSTTRNSLETSKAQEQQANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTAPKIPPGHDGNLVSGGSSAI

Target ORF information:

RefSeq Version	XM_017010418.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010418.2

ORF Insert Sequence:

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GGAGATAATT CCAGCCACAA AAGTGAAGTG 
CACGAGGTCC TAAGGCATGT GATGGAAATG AGTACAGTGC AGAGCACCAC CAGGAACAGC 
CTAGAAACGA GTAAAGCCCA GGAGCAGCAA GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCCCCTAAGA TCCCCCCAGG CCATGACGGA AACCTTGTTA GCGGTGGCAG TTCAGCAATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( XM_017010418.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865907.1 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu40187
Clone ID Related Accession (Same CDS sequence)	XM_005248895.3 , XM_017010418.2 , XM_017010418.1 , XM_005248895.4
Accession Version	XM_017010418.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GGAGATAATT CCAGCCACAA AAGTGAAGTG 
CACGAGGTCC TAAGGCATGT GATGGAAATG AGTACAGTGC AGAGCACCAC CAGGAACAGC 
CTAGAAACGA GTAAAGCCCA GGAGCAGCAA GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCCCCTAAGA TCCCCCCAGG CCATGACGGA AACCTTGTTA GCGGTGGCAG TTCAGCAATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865907.1
CDS	246..4388
Translation	MKSPRRTTLCLMFIVIYSSKAALNWNYESTIHPLSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEISFENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVCKLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVGDNSSHKSEVHEVLRHVMEMSTVQSTTRNSLETSKAQEQQANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTAPKIPPGHDGNLVSGGSSAI

Target ORF information:

RefSeq Version	XM_017010418.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010418.1

ORF Insert Sequence:

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GGAGATAATT CCAGCCACAA AAGTGAAGTG 
CACGAGGTCC TAAGGCATGT GATGGAAATG AGTACAGTGC AGAGCACCAC CAGGAACAGC 
CTAGAAACGA GTAAAGCCCA GGAGCAGCAA GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCCCCTAAGA TCCCCCCAGG CCATGACGGA AACCTTGTTA GCGGTGGCAG TTCAGCAATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( XM_005248895.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248952.1 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu40187
Clone ID Related Accession (Same CDS sequence)	XM_005248895.3 , XM_017010418.2 , XM_017010418.1 , XM_005248895.4
Accession Version	XM_005248895.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005248895.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GGAGATAATT CCAGCCACAA AAGTGAAGTG 
CACGAGGTCC TAAGGCATGT GATGGAAATG AGTACAGTGC AGAGCACCAC CAGGAACAGC 
CTAGAAACGA GTAAAGCCCA GGAGCAGCAA GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCCCCTAAGA TCCCCCCAGG CCATGACGGA AACCTTGTTA GCGGTGGCAG TTCAGCAATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248952.1
CDS	277..4419
Translation	MKSPRRTTLCLMFIVIYSSKAALNWNYESTIHPLSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEISFENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVCKLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVGDNSSHKSEVHEVLRHVMEMSTVQSTTRNSLETSKAQEQQANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTAPKIPPGHDGNLVSGGSSAI

Target ORF information:

RefSeq Version	XM_005248895.4
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248895.4

ORF Insert Sequence:

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GGAGATAATT CCAGCCACAA AAGTGAAGTG 
CACGAGGTCC TAAGGCATGT GATGGAAATG AGTACAGTGC AGAGCACCAC CAGGAACAGC 
CTAGAAACGA GTAAAGCCCA GGAGCAGCAA GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCCCCTAAGA TCCCCCCAGG CCATGACGGA AACCTTGTTA GCGGTGGCAG TTCAGCAATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( XM_017010419.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865908.1 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X7, mRNA.

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CloneID	OHu78034
Clone ID Related Accession (Same CDS sequence)	XM_017010419.2 , XM_011514353.3 , XM_011514353.2 , XM_017010419.1
Accession Version	XM_017010419.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017010419.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCAGCCCCTA AGATCCCCCC AGGCCATGAC GGAAACCTTG TTAGCGGTGG CAGTTCAGCA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865908.1
CDS	246..4271
Translation	MKSPRRTTLCLMFIVIYSSKAALNWNYESTIHPLSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEISFENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVCKLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTAAPKIPPGHDGNLVSGGSSAI

Target ORF information:

RefSeq Version	XM_017010419.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010419.2

ORF Insert Sequence:

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCAGCCCCTA AGATCCCCCC AGGCCATGAC GGAAACCTTG TTAGCGGTGG CAGTTCAGCA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( XM_011514353.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512655.1 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu78034
Clone ID Related Accession (Same CDS sequence)	XM_017010419.2 , XM_011514353.3 , XM_011514353.2 , XM_017010419.1
Accession Version	XM_011514353.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011514353.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCAGCCCCTA AGATCCCCCC AGGCCATGAC GGAAACCTTG TTAGCGGTGG CAGTTCAGCA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512655.1
CDS	277..4302
Translation	MKSPRRTTLCLMFIVIYSSKAALNWNYESTIHPLSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEISFENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVCKLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTAAPKIPPGHDGNLVSGGSSAI

Target ORF information:

RefSeq Version	XM_011514353.3
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514353.3

ORF Insert Sequence:

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCAGCCCCTA AGATCCCCCC AGGCCATGAC GGAAACCTTG TTAGCGGTGG CAGTTCAGCA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( XM_011514353.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512655.1 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu78034
Clone ID Related Accession (Same CDS sequence)	XM_017010419.2 , XM_011514353.3 , XM_011514353.2 , XM_017010419.1
Accession Version	XM_011514353.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514353.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCAGCCCCTA AGATCCCCCC AGGCCATGAC GGAAACCTTG TTAGCGGTGG CAGTTCAGCA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512655.1
CDS	290..4315
Translation	MKSPRRTTLCLMFIVIYSSKAALNWNYESTIHPLSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEISFENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVCKLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTAAPKIPPGHDGNLVSGGSSAI

Target ORF information:

RefSeq Version	XM_011514353.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514353.2

ORF Insert Sequence:

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCAGCCCCTA AGATCCCCCC AGGCCATGAC GGAAACCTTG TTAGCGGTGG CAGTTCAGCA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRF5 ( XM_017010419.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865908.1 Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X7, mRNA.

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CloneID	OHu78034
Clone ID Related Accession (Same CDS sequence)	XM_017010419.2 , XM_011514353.3 , XM_011514353.2 , XM_017010419.1
Accession Version	XM_017010419.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor F5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCAGCCCCTA AGATCCCCCC AGGCCATGAC GGAAACCTTG TTAGCGGTGG CAGTTCAGCA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865908.1
CDS	246..4271
Translation	MKSPRRTTLCLMFIVIYSSKAALNWNYESTIHPLSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEISFENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYGILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTPEIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVCKLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDIDSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVANLTITPDPISVSEGQNFSIKCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVIVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASSVSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPIGGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSLGAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGDSPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILAQDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDGDNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMRHTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILHETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITITIVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGLFILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTAAPKIPPGHDGNLVSGGSSAI

Target ORF information:

RefSeq Version	XM_017010419.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010419.1

ORF Insert Sequence:

ATGAAATCCC CAAGGAGAAC CACTTTGTGC CTCATGTTTA TTGTGATTTA TTCTTCCAAA 
GCTGCACTGA ACTGGAATTA CGAGTCTACT ATTCATCCTT TGAGTCTTCA TGAACATGAA 
CCAGCTGGTG AAGAGGCACT GAGGCAAAAA CGAGCCGTTG CCACAAAAAG TCCTACGGCT 
GAAGAATACA CTGTTAATAT TGAGATCAGT TTTGAAAATG CATCCTTCCT GGATCCTATC 
AAAGCCTACT TGAACAGCCT CAGTTTTCCA ATTCATGGGA ATAACACTGA CCAAATTACC 
GACATTTTGA GCATAAATGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC 
TCCTGCGAGA CAGGTTATGG GTGGCCTCGG GAAAGGTGTC TTCACAATCT CATTTGTCAA 
GAGCGTGACG TCTTCCTCCC AGGGCACCAT TGCAGTTGCC TTAAAGAACT GCCTCCCAAT 
GGACCTTTTT GCCTGCTTCA GGAAGATGTT ACCCTGAACA TGAGAGTCAG ACTAAATGTA 
GGCTTTCAAG AAGACCTCAT GAACACTTCC TCCGCCCTCT ATAGGTCCTA CAAGACCGAC 
TTGGAAACAG CGTTCCGGAA GGGTTACGGA ATTTTACCAG GCTTCAAGGG CGTGACTGTG 
ACAGGGTTCA AGTCTGGAAG TGTGGTTGTG ACATATGAAG TCAAGACTAC ACCACCATCA 
CTTGAGTTAA TACATAAAGC CAATGAACAA GTTGTACAGA GCCTCAATCA GACCTACAAA 
ATGGACTACA ACTCCTTTCA AGCAGTTACT ATCAATGAAA GCAATTTCTT TGTCACACCA 
GAAATCATCT TTGAAGGGGA CACAGTCAGT CTGGTGTGTG AAAAGGAAGT TTTGTCCTCC 
AATGTGTCTT GGCGCTATGA AGAACAGCAG TTGGAAATCC AGAACAGCAG CAGATTCTCG 
ATTTACACCG CACTTTTCAA CAACATGACT TCGGTGTCCA AGCTCACCAT CCACAACATC 
ACTCCAGGTG ATGCAGGTGA ATATGTTTGC AAACTGATAT TAGACATTTT TGAATATGAG 
TGCAAGAAGA AAATAGATGT TATGCCCATC CAAATTTTGG CAAATGAAGA AATGAAGGTG 
ATGTGCGACA ACAATCCTGT ATCTTTGAAC TGCTGCAGTC AGGGTAATGT TAATTGGAGC 
AAAGTAGAAT GGAAGCAGGA AGGAAAAATA AATATTCCAG GAACCCCTGA GACAGACATA 
GATTCTAGCT GCAGCAGATA CACCCTCAAG GCTGATGGAA CCCAGTGCCC AAGCGGGTCG 
TCTGGAACAA CAGTCATCTA CACTTGTGAG TTCATCAGTG CCTATGGAGC CAGAGGCAGT 
GCAAACATAA AAGTGACATT CATCTCTGTG GCCAATCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAA CTTTTCTATA AAATGCATCA GTGATGTGAG TAACTATGAT 
GAGGTTTATT GGAACACTTC TGCTGGAATT AAAATATACC AAAGATTTTA TACCACGAGG 
AGGTATCTTG ATGGAGCAGA ATCAGTACTG ACAGTCAAGA CCTCGACCAG GGAGTGGAAT 
GGAACCTATC ACTGCATATT TAGATATAAG AATTCATACA GTATTGCAAC CAAAGACGTC 
ATTGTTCACC CGCTGCCTCT AAAGCTGAAC ATCATGGTTG ATCCTTTGGA AGCTACTGTT 
TCATGCAGTG GTTCCCATCA CATCAAGTGC TGCATAGAGG AGGATGGAGA CTACAAAGTT 
ACTTTCCATA CGGGTTCCTC ATCCCTTCCT GCTGCAAAAG AAGTTAACAA AAAACAAGTG 
TGCTACAAAC ACAATTTCAA TGCAAGCTCA GTTTCCTGGT GTTCAAAAAC TGTTGATGTG 
TGTTGTCACT TTACCAATGC TGCTAATAAT TCAGTCTGGA GCCCATCTAT GAAGCTGAAT 
CTGGTTCCTG GGGAAAACAT CACATGCCAG GATCCCGTAA TAGGTGTCGG AGAGCCGGGG 
AAAGTCATCC AGAAGCTATG CCGGTTCTCA AACGTTCCCA GCAGCCCTGA GAGTCCCATT 
GGCGGGACCA TCACTTACAA ATGTGTAGGC TCCCAGTGGG AGGAGAAGAG AAATGACTGC 
ATCTCTGCCC CAATAAACAG TCTGCTCCAG ATGGCTAAGG CTTTGATCAA GAGCCCCTCT 
CAGGATGAGA TGCTCCCTAC ATACCTGAAG GATCTTTCTA TTAGCATAGA CAAAGCGGAA 
CATGAAATCA GCTCTTCTCC TGGGAGTCTG GGAGCCATTA TTAACATCCT TGATCTGCTC 
TCAACAGTTC CAACCCAAGT AAATTCAGAA ATGATGACGC ACGTGCTCTC TACGGTTAAT 
GTCATCCTTG GCAAGCCCGT CTTGAACACC TGGAAGGTTT TACAACAGCA ATGGACCAAT 
CAGAGTTCAC AGCTACTACA TTCAGTGGAA AGATTTTCCC AAGCATTACA GTCGGGAGAT 
AGCCCTCCTT TGTCCTTCTC CCAAACTAAT GTGCAGATGA GCAGCATGGT AATCAAGTCC 
AGCCACCCAG AAACCTATCA ACAGAGGTTT GTTTTCCCAT ACTTTGACCT CTGGGGCAAT 
GTGGTCATTG ACAAGAGCTA TCTAGAAAAC TTGCAGTCGG ATTCGTCTAT TGTCACCATG 
GCTTTCCCAA CTCTCCAAGC CATCCTTGCC CAGGATATCC AGGAAAATAA CTTTGCAGAG 
AGCTTAGTGA TGACAACCAC TGTCAGCCAC AATACAACTA TGCCATTCAG GATTTCAATG 
ACTTTTAAGA ACAATAGCCC TTCAGGCGGC GAAACGAAGT GTGTCTTCTG GAACTTCAGG 
CTTGCCAACA ACACAGGGGG GTGGGACAGC AGTGGGTGCT ATGTAGAAGA AGGTGATGGG 
GACAATGTCA CCTGTATCTG TGACCACCTA ACATCATTCT CCATCCTCAT GTCCCCTGAC 
TCCCCAGATC CTAGTTCTCT CCTGGGAATA CTCCTGGATA TTATTTCTTA TGTTGGGGTG 
GGCTTTTCCA TCTTGAGCTT GGCAGCCTGT CTAGTTGTGG AAGCTGTGGT GTGGAAATCG 
GTGACCAAGA ACCGGACTTC TTATATGCGC CACACCTGCA TAGTGAATAT CGCTGCCTCC 
CTTCTGGTCG CCAACACCTG GTTCATTGTG GTCGCTGCCA TCCAGGACAA TCGCTACATA 
CTCTGCAAGA CAGCCTGTGT GGCTGCCACC TTCTTCATCC ACTTCTTCTA CCTCAGCGTC 
TTCTTCTGGA TGCTGACACT GGGCCTCATG CTGTTCTATC GCCTGGTTTT CATTCTGCAT 
GAAACAAGCA GGTCCACTCA GAAAGCCATT GCCTTCTGTC TTGGCTATGG CTGCCCACTT 
GCCATCTCGG TCATCACGCT GGGAGCCACC CAGCCCCGGG AAGTCTATAC GAGGAAGAAT 
GTCTGTTGGC TCAACTGGGA GGACACCAAG GCCCTGCTGG CTTTCGCCAT CCCAGCACTG 
ATCATTGTGG TGGTGAACAT AACCATCACT ATTGTGGTCA TCACCAAGAT CCTGAGGCCT 
TCCATTGGAG ACAAGCCATG CAAGCAGGAG AAGAGCAGCC TGTTTCAGAT CAGCAAGAGC 
ATTGGGGTCC TCACACCACT CTTGGGCCTC ACTTGGGGTT TTGGTCTCAC CACTGTGTTC 
CCAGGGACCA ACCTTGTGTT CCATATCATA TTTGCCATCC TCAATGTCTT CCAGGGATTA 
TTCATTTTAC TCTTTGGATG CCTCTGGGAT CTGAAGGTAC AGGAAGCTTT GCTGAATAAG 
TTTTCATTGT CGAGATGGTC TTCACAGCAC TCAAAGTCAA CATCCCTGGG TTCATCCACA 
CCTGTGTTTT CTATGAGTTC TCCAATATCA AGGAGATTTA ACAATTTGTT TGGTAAAACA 
GCAGCCCCTA AGATCCCCCC AGGCCATGAC GGAAACCTTG TTAGCGGTGG CAGTTCAGCA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High expression of GPR116 indicates poor survival outcome and promotes tumor progression in colorectal carcinoma.
Oncotarget8(29)47943-47956(2017 Jul)
Yang L,Lin XL,Liang W,Fu SW,Lin WF,Tian XQ,Gao YJ,Chen HY,Dai J,Ge ZZ

International Union of Basic and Clinical Pharmacology. XCIV. Adhesion G protein-coupled receptors.
Pharmacological reviews67(2)338-67(2015)
Hamann J,Aust G,Ara? D,Engel FB,Formstone C,Fredriksson R,Hall RA,Harty BL,Kirchhoff C,Knapp B,Krishnan A,Liebscher I,Lin HH,Martinelli DC,Monk KR,Peeters MC,Piao X,Pr?mel S,Sch?neberg T,Schwartz TW,Singer K,Stacey M,Ushkaryov YA,Vallon M,Wolfrum U,Wright MW,Xu L,Langenhan T,Schi?th HB

GPR116, an adhesion G-protein-coupled receptor, promotes breast cancer metastasis via the G?q-p63RhoGEF-Rho GTPase pathway.
Cancer research73(20)6206-18(2013 Oct)
Tang X,Jin R,Qu G,Wang X,Li Z,Yuan Z,Zhao C,Siwko S,Shi T,Wang P,Xiao J,Liu M,Luo J

Essential regulation of lung surfactant homeostasis by the orphan G protein-coupled receptor GPR116.
Cell reports3(5)1457-64(2013 May)
Yang MY,Hilton MB,Seaman S,Haines DC,Nagashima K,Burks CM,Tessarollo L,Ivanova PT,Brown HA,Umstead TM,Floros J,Chroneos ZC,St Croix B

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Diabetes care33(10)2250-3(2010 Oct)
Bailey SD,Xie C,Do R,Montpetit A,Diaz R,Mohan V,Keavney B,Yusuf S,Gerstein HC,Engert JC,Anand S,

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ADGRF5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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