DFFA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DFFA
Entrez Gene ID	1676
Full Name	DNA fragmentation factor subunit alpha
Synonyms	DFF-45,DFF1,ICAD
General protein information	Preferred Names DNA fragmentation factor subunit alpha Names DNA fragmentation factor subunit alpha DFF45 DNA fragmentation factor 45 kDa subunit DNA fragmentation factor, 45kDa, alpha polypeptide inhibitor of CAD
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.22
Summary	Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	601882

mRNA and Protein(s)

mRNA	Protein	Name
NM_213566.1	NP_998731.1	DNA fragmentation factor subunit alpha isoform 2
NM_004401.3	NP_004392.1	DNA fragmentation factor subunit alpha isoform 1
NM_213566.2	NP_998731.1	DNA fragmentation factor subunit alpha isoform 2
NM_004401.2	NP_004392.1	DNA fragmentation factor subunit alpha isoform 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Dffa	NP_446131.2
Gallus gallus (chicken)	DFFA	XP_417610.1
Bos taurus (cattle)	DFFA	NP_001068810.1
	DFFA	XP_001102195.1
Canis lupus familiaris (dog)	DFFA	XP_544574.2
Danio rerio (zebrafish)	dffa	NP_001002631.1
Homo sapiens (human)	DFFA	NP_004392.1
Pan troglodytes (chimpanzee)	DFFA	XP_001161941.1
Mus musculus (house mouse)	Dffa	NP_001020467.1
Xenopus tropicalis (tropical clawed frog)	dffa	XP_002939454.2

Gene Ontology
Bibliography

Related articles in PubMed

Endometrial Polyps and Benign Endometrial Hyperplasia Present Increased Prevalence of DNA Fragmentation Factors 40 and 45 (DFF40 and DFF45) Together With the Antiapoptotic B-Cell Lymphoma (Bcl-2) Protein Compared With Normal Human Endometria.
Banas T, Pitynski K, Mikos M, Cielecka-Kuszyk J
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists(2017 Sep)

Evaluation of miRNA-196a2 and apoptosis-related target genes: ANXA1, DFFA and PDCD4 expression in gastrointestinal cancer patients: A pilot study.
Fawzy MS, Toraih EA, Ibrahiem A, Abdeldayem H, Mohamed AO, Abdel-Daim MM
PloS one12(11)e0187310(2017)

Engineered cellular gene-replacement platform for selective and inducible proteolytic profiling.
Morgan CW, Diaz JE, Zeitlin SG, Gray DC, Wells JA
Proceedings of the National Academy of Sciences of the United States of America112(27)8344-9(2015 Jul)

The functional landscape of Hsp27 reveals new cellular processes such as DNA repair and alternative splicing and proposes novel anticancer targets.
Katsogiannou M, Andrieu C, Baylot V, Baudot A, Dusetti NJ, Gayet O, Finetti P, Garrido C, Birnbaum D, Bertucci F, Brun C, Rocchi P
Molecular & cellular proteomics : MCP13(12)3585-601(2014 Dec)

siRNA-mediated knock-down of DFF45 amplifies doxorubicin therapeutic effects in breast cancer cells.
Bagheri F, Safarian S, Eslaminejad MB, Sheibani N
Cellular oncology (Dordrecht)36(6)515-26(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

evaluate the relative expression levels of miR-196a2 and three of its selected apoptosis-related targets; ANXA1, DFFA and PDCD4 in a sample of GI cancer patients
Title: Evaluation of miRNA-196a2 and apoptosis-related target genes: ANXA1, DFFA and PDCD4 expression in gastrointestinal cancer patients: A pilot study.

Data show that the caspase-activated DNase (CAD) is activated when caspases cleave its endogenous inhibitor ICAD, resulting in the characteristic DNA laddering of apoptosis.
Title: Engineered cellular gene-replacement platform for selective and inducible proteolytic profiling.

Data suggest that DFF45 gene silencing, when applied in combination with doxorubicin, may offer a therapeutic strategy for the treatment of breast cancer.
Title: siRNA-mediated knock-down of DFF45 amplifies doxorubicin therapeutic effects in breast cancer cells.

ICAD deficiency was associated with severe genomic instability.
Title: ICAD deficiency in human colon cancer and predisposition to colon tumorigenesis: linkage to apoptosis resistance and genomic instability.

mRNA splicing is actively driven toward the pro-apoptotic isoforms of Bim, Bcl-x, and ICAD in Pnn-depleted MCF-7 cells.
Title: Loss of Pnn expression results in mouse early embryonic lethality and cellular apoptosis through SRSF1-mediated alternative expression of Bcl-xS and ICAD.

The following DFFA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DFFA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18319	NM_004401.3 Latest version!	Homo sapiens DNA fragmentation factor subunit alpha (DFFA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu03206	NM_213566.2 Latest version!	Homo sapiens DNA fragmentation factor subunit alpha (DFFA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
View Old Accession Versions >>
OHu03206	NM_213566.1	Homo sapiens DNA fragmentation factor subunit alpha (DFFA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OHu18319	NM_004401.2	Homo sapiens DNA fragmentation factor subunit alpha (DFFA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DFFA ( NM_004401.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004392.1 Homo sapiens DNA fragmentation factor subunit alpha (DFFA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18319
Clone ID Related Accession (Same CDS sequence)	NM_004401.3 , NM_004401.2
Accession Version	NM_004401.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	DNA fragmentation factor subunit alpha isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU121791.1, BC007112.1, BC000037.2, BC007721.2 and AL354956.13. On Nov 23, 2018 this sequence version replaced NM_004401.2. Transcript Variant: This variant (1) represents the shorter transcript but encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.376436.1, BC007721.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000377038.8/ ENSP00000366237.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGTGA CCGGGGACGC CGGGGTACCA GAATCTGGCG AGATCCGGAC TCTAAAGCCG 
TGTCTGCTGC GCCGCAACTA CAGCCGCGAA CAGCACGGCG TGGCCGCCTC CTGCCTCGAA 
GACCTGAGGA GCAAGGCCTG TGACATTCTG GCCATTGATA AGTCCCTGAC ACCAGTCACC 
CTGGTCCTGG CAGAGGATGG CACCATAGTG GATGATGACG ATTACTTTCT GTGTCTACCT 
TCCAATACTA AGTTTGTGGC ATTGGCTAGT AATGAGAAAT GGGCATACAA CAATTCAGAT 
GGAGGTACAG CTTGGATTTC CCAAGAGTCC TTTGATGTAG ATGAAACAGA CAGCGGGGCA 
GGGTTGAAGT GGAAGAATGT GGCCAGGCAG CTGAAAGAAG ATCTGTCCAG CATCATCCTC 
CTATCAGAGG AGGACCTCCA GATGCTTGTT GACGCTCCCT GCTCAGACCT GGCTCAGGAA 
CTACGTCAGA GTTGTGCCAC CGTCCAGCGG CTGCAGCACA CACTCCAACA GGTGCTTGAC 
CAAAGAGAGG AAGTGCGTCA GTCCAAGCAG CTCCTGCAGC TGTACCTCCA GGCTTTGGAG 
AAAGAGGGCA GCCTCTTGTC AAAGCAGGAA GAGTCCAAAG CTGCCTTTGG TGAGGAGGTG 
GATGCAGTAG ACACGGGTAT CAGCAGAGAG ACCTCCTCGG ACGTTGCGCT GGCGAGCCAC 
ATCCTTACTG CACTGAGGGA GAAGCAGGCT CCAGAGCTGA GCTTATCTAG TCAGGATTTG 
GAGTTGGTTA CCAAGGAAGA CCCCAAAGCA CTGGCTGTTG CCTTGAACTG GGACATAAAG 
AAGACGGAGA CTGTTCAGGA GGCCTGTGAG CGGGAGCTCG CCCTGCGCCT GCAGCAGACG 
CAGAGCTTGC ATTCTCTCCG GAGCATCTCA GCAAGCAAGG CCTCACCACC TGGTGACCTG 
CAGAATCCTA AGCGAGCCAG ACAGGATCCC ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004392.1
CDS	72..1067
Translation	MEVTGDAGVPESGEIRTLKPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDKSLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWAYNNSDGGTAWISQESFDVDETDSGAGLKWKNVARQLKEDLSSIILLSEEDLQMLVDAPCSDLAQELRQSCATVQRLQHTLQQVLDQREEVRQSKQLLQLYLQALEKEGSLLSKQEESKAAFGEEVDAVDTGISRETSSDVALASHILTALREKQAPELSLSSQDLELVTKEDPKALAVALNWDIKKTETVQEACERELALRLQQTQSLHSLRSISASKASPPGDLQNPKRARQDPT

Target ORF information:

RefSeq Version	NM_004401.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA fragmentation factor subunit alpha (DFFA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004401.3

ORF Insert Sequence:

ATGGAGGTGA CCGGGGACGC CGGGGTACCA GAATCTGGCG AGATCCGGAC TCTAAAGCCG 
TGTCTGCTGC GCCGCAACTA CAGCCGCGAA CAGCACGGCG TGGCCGCCTC CTGCCTCGAA 
GACCTGAGGA GCAAGGCCTG TGACATTCTG GCCATTGATA AGTCCCTGAC ACCAGTCACC 
CTGGTCCTGG CAGAGGATGG CACCATAGTG GATGATGACG ATTACTTTCT GTGTCTACCT 
TCCAATACTA AGTTTGTGGC ATTGGCTAGT AATGAGAAAT GGGCATACAA CAATTCAGAT 
GGAGGTACAG CTTGGATTTC CCAAGAGTCC TTTGATGTAG ATGAAACAGA CAGCGGGGCA 
GGGTTGAAGT GGAAGAATGT GGCCAGGCAG CTGAAAGAAG ATCTGTCCAG CATCATCCTC 
CTATCAGAGG AGGACCTCCA GATGCTTGTT GACGCTCCCT GCTCAGACCT GGCTCAGGAA 
CTACGTCAGA GTTGTGCCAC CGTCCAGCGG CTGCAGCACA CACTCCAACA GGTGCTTGAC 
CAAAGAGAGG AAGTGCGTCA GTCCAAGCAG CTCCTGCAGC TGTACCTCCA GGCTTTGGAG 
AAAGAGGGCA GCCTCTTGTC AAAGCAGGAA GAGTCCAAAG CTGCCTTTGG TGAGGAGGTG 
GATGCAGTAG ACACGGGTAT CAGCAGAGAG ACCTCCTCGG ACGTTGCGCT GGCGAGCCAC 
ATCCTTACTG CACTGAGGGA GAAGCAGGCT CCAGAGCTGA GCTTATCTAG TCAGGATTTG 
GAGTTGGTTA CCAAGGAAGA CCCCAAAGCA CTGGCTGTTG CCTTGAACTG GGACATAAAG 
AAGACGGAGA CTGTTCAGGA GGCCTGTGAG CGGGAGCTCG CCCTGCGCCT GCAGCAGACG 
CAGAGCTTGC ATTCTCTCCG GAGCATCTCA GCAAGCAAGG CCTCACCACC TGGTGACCTG 
CAGAATCCTA AGCGAGCCAG ACAGGATCCC ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DFFA ( NM_004401.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_004392.1 Homo sapiens DNA fragmentation factor subunit alpha (DFFA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18319
Clone ID Related Accession (Same CDS sequence)	NM_004401.3 , NM_004401.2
Accession Version	NM_004401.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	DNA fragmentation factor subunit alpha isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU121791.1, BC007112.1, BC000037.2 and BC007721.2. This sequence is a reference standard in the RefSeqGene project. On May 12, 2004 this sequence version replaced NM_004401.1. Transcript Variant: This variant (1) represents the shorter transcript but encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC007721.2, SRR1163655.373043.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGTGA CCGGGGACGC CGGGGTACCA GAATCTGGCG AGATCCGGAC TCTAAAGCCG 
TGTCTGCTGC GCCGCAACTA CAGCCGCGAA CAGCACGGCG TGGCCGCCTC CTGCCTCGAA 
GACCTGAGGA GCAAGGCCTG TGACATTCTG GCCATTGATA AGTCCCTGAC ACCAGTCACC 
CTGGTCCTGG CAGAGGATGG CACCATAGTG GATGATGACG ATTACTTTCT GTGTCTACCT 
TCCAATACTA AGTTTGTGGC ATTGGCTAGT AATGAGAAAT GGGCATACAA CAATTCAGAT 
GGAGGTACAG CTTGGATTTC CCAAGAGTCC TTTGATGTAG ATGAAACAGA CAGCGGGGCA 
GGGTTGAAGT GGAAGAATGT GGCCAGGCAG CTGAAAGAAG ATCTGTCCAG CATCATCCTC 
CTATCAGAGG AGGACCTCCA GATGCTTGTT GACGCTCCCT GCTCAGACCT GGCTCAGGAA 
CTACGTCAGA GTTGTGCCAC CGTCCAGCGG CTGCAGCACA CACTCCAACA GGTGCTTGAC 
CAAAGAGAGG AAGTGCGTCA GTCCAAGCAG CTCCTGCAGC TGTACCTCCA GGCTTTGGAG 
AAAGAGGGCA GCCTCTTGTC AAAGCAGGAA GAGTCCAAAG CTGCCTTTGG TGAGGAGGTG 
GATGCAGTAG ACACGGGTAT CAGCAGAGAG ACCTCCTCGG ACGTTGCGCT GGCGAGCCAC 
ATCCTTACTG CACTGAGGGA GAAGCAGGCT CCAGAGCTGA GCTTATCTAG TCAGGATTTG 
GAGTTGGTTA CCAAGGAAGA CCCCAAAGCA CTGGCTGTTG CCTTGAACTG GGACATAAAG 
AAGACGGAGA CTGTTCAGGA GGCCTGTGAG CGGGAGCTCG CCCTGCGCCT GCAGCAGACG 
CAGAGCTTGC ATTCTCTCCG GAGCATCTCA GCAAGCAAGG CCTCACCACC TGGTGACCTG 
CAGAATCCTA AGCGAGCCAG ACAGGATCCC ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004392.1
CDS	99..1094
Translation	MEVTGDAGVPESGEIRTLKPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDKSLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWAYNNSDGGTAWISQESFDVDETDSGAGLKWKNVARQLKEDLSSIILLSEEDLQMLVDAPCSDLAQELRQSCATVQRLQHTLQQVLDQREEVRQSKQLLQLYLQALEKEGSLLSKQEESKAAFGEEVDAVDTGISRETSSDVALASHILTALREKQAPELSLSSQDLELVTKEDPKALAVALNWDIKKTETVQEACERELALRLQQTQSLHSLRSISASKASPPGDLQNPKRARQDPT

Target ORF information:

RefSeq Version	NM_004401.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA fragmentation factor subunit alpha (DFFA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004401.2

ORF Insert Sequence:

ATGGAGGTGA CCGGGGACGC CGGGGTACCA GAATCTGGCG AGATCCGGAC TCTAAAGCCG 
TGTCTGCTGC GCCGCAACTA CAGCCGCGAA CAGCACGGCG TGGCCGCCTC CTGCCTCGAA 
GACCTGAGGA GCAAGGCCTG TGACATTCTG GCCATTGATA AGTCCCTGAC ACCAGTCACC 
CTGGTCCTGG CAGAGGATGG CACCATAGTG GATGATGACG ATTACTTTCT GTGTCTACCT 
TCCAATACTA AGTTTGTGGC ATTGGCTAGT AATGAGAAAT GGGCATACAA CAATTCAGAT 
GGAGGTACAG CTTGGATTTC CCAAGAGTCC TTTGATGTAG ATGAAACAGA CAGCGGGGCA 
GGGTTGAAGT GGAAGAATGT GGCCAGGCAG CTGAAAGAAG ATCTGTCCAG CATCATCCTC 
CTATCAGAGG AGGACCTCCA GATGCTTGTT GACGCTCCCT GCTCAGACCT GGCTCAGGAA 
CTACGTCAGA GTTGTGCCAC CGTCCAGCGG CTGCAGCACA CACTCCAACA GGTGCTTGAC 
CAAAGAGAGG AAGTGCGTCA GTCCAAGCAG CTCCTGCAGC TGTACCTCCA GGCTTTGGAG 
AAAGAGGGCA GCCTCTTGTC AAAGCAGGAA GAGTCCAAAG CTGCCTTTGG TGAGGAGGTG 
GATGCAGTAG ACACGGGTAT CAGCAGAGAG ACCTCCTCGG ACGTTGCGCT GGCGAGCCAC 
ATCCTTACTG CACTGAGGGA GAAGCAGGCT CCAGAGCTGA GCTTATCTAG TCAGGATTTG 
GAGTTGGTTA CCAAGGAAGA CCCCAAAGCA CTGGCTGTTG CCTTGAACTG GGACATAAAG 
AAGACGGAGA CTGTTCAGGA GGCCTGTGAG CGGGAGCTCG CCCTGCGCCT GCAGCAGACG 
CAGAGCTTGC ATTCTCTCCG GAGCATCTCA GCAAGCAAGG CCTCACCACC TGGTGACCTG 
CAGAATCCTA AGCGAGCCAG ACAGGATCCC ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DFFA ( NM_213566.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_998731.1 Homo sapiens DNA fragmentation factor subunit alpha (DFFA), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu03206
Clone ID Related Accession (Same CDS sequence)	NM_213566.1 , NM_213566.2
Accession Version	NM_213566.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	DNA fragmentation factor subunit alpha isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU121791.1, AF087573.1, BE207821.1, BU956486.1, BC007112.1, BC000037.2 and BC007721.2. Transcript Variant: This variant (2) has an additional segment in the 3' region, as compared to variant 1. It encodes isoform 2 which has a shorter and distinct C-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AU121791.1, SRR1803615.268893.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGTGA CCGGGGACGC CGGGGTACCA GAATCTGGCG AGATCCGGAC TCTAAAGCCG 
TGTCTGCTGC GCCGCAACTA CAGCCGCGAA CAGCACGGCG TGGCCGCCTC CTGCCTCGAA 
GACCTGAGGA GCAAGGCCTG TGACATTCTG GCCATTGATA AGTCCCTGAC ACCAGTCACC 
CTGGTCCTGG CAGAGGATGG CACCATAGTG GATGATGACG ATTACTTTCT GTGTCTACCT 
TCCAATACTA AGTTTGTGGC ATTGGCTAGT AATGAGAAAT GGGCATACAA CAATTCAGAT 
GGAGGTACAG CTTGGATTTC CCAAGAGTCC TTTGATGTAG ATGAAACAGA CAGCGGGGCA 
GGGTTGAAGT GGAAGAATGT GGCCAGGCAG CTGAAAGAAG ATCTGTCCAG CATCATCCTC 
CTATCAGAGG AGGACCTCCA GATGCTTGTT GACGCTCCCT GCTCAGACCT GGCTCAGGAA 
CTACGTCAGA GTTGTGCCAC CGTCCAGCGG CTGCAGCACA CACTCCAACA GGTGCTTGAC 
CAAAGAGAGG AAGTGCGTCA GTCCAAGCAG CTCCTGCAGC TGTACCTCCA GGCTTTGGAG 
AAAGAGGGCA GCCTCTTGTC AAAGCAGGAA GAGTCCAAAG CTGCCTTTGG TGAGGAGGTG 
GATGCAGTAG ACACGGGTAT CAGCAGAGAG ACCTCCTCGG ACGTTGCGCT GGCGAGCCAC 
ATCCTTACTG CACTGAGGGA GAAGCAGGCT CCAGAGCTGA GCTTATCTAG TCAGGATTTG 
GAGGTGGGCG GAAACCAGGG TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998731.1
CDS	99..905
Translation	MEVTGDAGVPESGEIRTLKPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDKSLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWAYNNSDGGTAWISQESFDVDETDSGAGLKWKNVARQLKEDLSSIILLSEEDLQMLVDAPCSDLAQELRQSCATVQRLQHTLQQVLDQREEVRQSKQLLQLYLQALEKEGSLLSKQEESKAAFGEEVDAVDTGISRETSSDVALASHILTALREKQAPELSLSSQDLEVGGNQGH

Target ORF information:

RefSeq Version	NM_213566.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA fragmentation factor subunit alpha (DFFA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213566.1

ORF Insert Sequence:

ATGGAGGTGA CCGGGGACGC CGGGGTACCA GAATCTGGCG AGATCCGGAC TCTAAAGCCG 
TGTCTGCTGC GCCGCAACTA CAGCCGCGAA CAGCACGGCG TGGCCGCCTC CTGCCTCGAA 
GACCTGAGGA GCAAGGCCTG TGACATTCTG GCCATTGATA AGTCCCTGAC ACCAGTCACC 
CTGGTCCTGG CAGAGGATGG CACCATAGTG GATGATGACG ATTACTTTCT GTGTCTACCT 
TCCAATACTA AGTTTGTGGC ATTGGCTAGT AATGAGAAAT GGGCATACAA CAATTCAGAT 
GGAGGTACAG CTTGGATTTC CCAAGAGTCC TTTGATGTAG ATGAAACAGA CAGCGGGGCA 
GGGTTGAAGT GGAAGAATGT GGCCAGGCAG CTGAAAGAAG ATCTGTCCAG CATCATCCTC 
CTATCAGAGG AGGACCTCCA GATGCTTGTT GACGCTCCCT GCTCAGACCT GGCTCAGGAA 
CTACGTCAGA GTTGTGCCAC CGTCCAGCGG CTGCAGCACA CACTCCAACA GGTGCTTGAC 
CAAAGAGAGG AAGTGCGTCA GTCCAAGCAG CTCCTGCAGC TGTACCTCCA GGCTTTGGAG 
AAAGAGGGCA GCCTCTTGTC AAAGCAGGAA GAGTCCAAAG CTGCCTTTGG TGAGGAGGTG 
GATGCAGTAG ACACGGGTAT CAGCAGAGAG ACCTCCTCGG ACGTTGCGCT GGCGAGCCAC 
ATCCTTACTG CACTGAGGGA GAAGCAGGCT CCAGAGCTGA GCTTATCTAG TCAGGATTTG 
GAGGTGGGCG GAAACCAGGG TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DFFA ( NM_213566.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_998731.1 Homo sapiens DNA fragmentation factor subunit alpha (DFFA), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu03206
Clone ID Related Accession (Same CDS sequence)	NM_213566.1 , NM_213566.2
Accession Version	NM_213566.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	DNA fragmentation factor subunit alpha isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU121791.1, AF087573.1, BE207821.1, BU956486.1, BC007112.1, BC000037.2 and BC007721.2. On May 31, 2019 this sequence version replaced NM_213566.1. Transcript Variant: This variant (2) has an additional segment in the 3' region, as compared to variant 1. It encodes isoform 2 which has a shorter and distinct C-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.257755.1, SRR1803615.268893.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGTGA CCGGGGACGC CGGGGTACCA GAATCTGGCG AGATCCGGAC TCTAAAGCCG 
TGTCTGCTGC GCCGCAACTA CAGCCGCGAA CAGCACGGCG TGGCCGCCTC CTGCCTCGAA 
GACCTGAGGA GCAAGGCCTG TGACATTCTG GCCATTGATA AGTCCCTGAC ACCAGTCACC 
CTGGTCCTGG CAGAGGATGG CACCATAGTG GATGATGACG ATTACTTTCT GTGTCTACCT 
TCCAATACTA AGTTTGTGGC ATTGGCTAGT AATGAGAAAT GGGCATACAA CAATTCAGAT 
GGAGGTACAG CTTGGATTTC CCAAGAGTCC TTTGATGTAG ATGAAACAGA CAGCGGGGCA 
GGGTTGAAGT GGAAGAATGT GGCCAGGCAG CTGAAAGAAG ATCTGTCCAG CATCATCCTC 
CTATCAGAGG AGGACCTCCA GATGCTTGTT GACGCTCCCT GCTCAGACCT GGCTCAGGAA 
CTACGTCAGA GTTGTGCCAC CGTCCAGCGG CTGCAGCACA CACTCCAACA GGTGCTTGAC 
CAAAGAGAGG AAGTGCGTCA GTCCAAGCAG CTCCTGCAGC TGTACCTCCA GGCTTTGGAG 
AAAGAGGGCA GCCTCTTGTC AAAGCAGGAA GAGTCCAAAG CTGCCTTTGG TGAGGAGGTG 
GATGCAGTAG ACACGGGTAT CAGCAGAGAG ACCTCCTCGG ACGTTGCGCT GGCGAGCCAC 
ATCCTTACTG CACTGAGGGA GAAGCAGGCT CCAGAGCTGA GCTTATCTAG TCAGGATTTG 
GAGGTGGGCG GAAACCAGGG TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998731.1
CDS	72..878
Translation	MEVTGDAGVPESGEIRTLKPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDKSLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWAYNNSDGGTAWISQESFDVDETDSGAGLKWKNVARQLKEDLSSIILLSEEDLQMLVDAPCSDLAQELRQSCATVQRLQHTLQQVLDQREEVRQSKQLLQLYLQALEKEGSLLSKQEESKAAFGEEVDAVDTGISRETSSDVALASHILTALREKQAPELSLSSQDLEVGGNQGH

Target ORF information:

RefSeq Version	NM_213566.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA fragmentation factor subunit alpha (DFFA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213566.2

ORF Insert Sequence:

ATGGAGGTGA CCGGGGACGC CGGGGTACCA GAATCTGGCG AGATCCGGAC TCTAAAGCCG 
TGTCTGCTGC GCCGCAACTA CAGCCGCGAA CAGCACGGCG TGGCCGCCTC CTGCCTCGAA 
GACCTGAGGA GCAAGGCCTG TGACATTCTG GCCATTGATA AGTCCCTGAC ACCAGTCACC 
CTGGTCCTGG CAGAGGATGG CACCATAGTG GATGATGACG ATTACTTTCT GTGTCTACCT 
TCCAATACTA AGTTTGTGGC ATTGGCTAGT AATGAGAAAT GGGCATACAA CAATTCAGAT 
GGAGGTACAG CTTGGATTTC CCAAGAGTCC TTTGATGTAG ATGAAACAGA CAGCGGGGCA 
GGGTTGAAGT GGAAGAATGT GGCCAGGCAG CTGAAAGAAG ATCTGTCCAG CATCATCCTC 
CTATCAGAGG AGGACCTCCA GATGCTTGTT GACGCTCCCT GCTCAGACCT GGCTCAGGAA 
CTACGTCAGA GTTGTGCCAC CGTCCAGCGG CTGCAGCACA CACTCCAACA GGTGCTTGAC 
CAAAGAGAGG AAGTGCGTCA GTCCAAGCAG CTCCTGCAGC TGTACCTCCA GGCTTTGGAG 
AAAGAGGGCA GCCTCTTGTC AAAGCAGGAA GAGTCCAAAG CTGCCTTTGG TGAGGAGGTG 
GATGCAGTAG ACACGGGTAT CAGCAGAGAG ACCTCCTCGG ACGTTGCGCT GGCGAGCCAC 
ATCCTTACTG CACTGAGGGA GAAGCAGGCT CCAGAGCTGA GCTTATCTAG TCAGGATTTG 
GAGGTGGGCG GAAACCAGGG TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Endometrial Polyps and Benign Endometrial Hyperplasia Present Increased Prevalence of DNA Fragmentation Factors 40 and 45 (DFF40 and DFF45) Together With the Antiapoptotic B-Cell Lymphoma (Bcl-2) Protein Compared With Normal Human Endometria.
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists(2017 Sep)
Banas T,Pitynski K,Mikos M,Cielecka-Kuszyk J

Evaluation of miRNA-196a2 and apoptosis-related target genes: ANXA1, DFFA and PDCD4 expression in gastrointestinal cancer patients: A pilot study.
PloS one12(11)e0187310(2017)
Fawzy MS,Toraih EA,Ibrahiem A,Abdeldayem H,Mohamed AO,Abdel-Daim MM

Engineered cellular gene-replacement platform for selective and inducible proteolytic profiling.
Proceedings of the National Academy of Sciences of the United States of America112(27)8344-9(2015 Jul)
Morgan CW,Diaz JE,Zeitlin SG,Gray DC,Wells JA

The functional landscape of Hsp27 reveals new cellular processes such as DNA repair and alternative splicing and proposes novel anticancer targets.
Molecular & cellular proteomics : MCP13(12)3585-601(2014 Dec)
Katsogiannou M,Andrieu C,Baylot V,Baudot A,Dusetti NJ,Gayet O,Finetti P,Garrido C,Birnbaum D,Bertucci F,Brun C,Rocchi P

siRNA-mediated knock-down of DFF45 amplifies doxorubicin therapeutic effects in breast cancer cells.
Cellular oncology (Dordrecht)36(6)515-26(2013 Dec)
Bagheri F,Safarian S,Eslaminejad MB,Sheibani N

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DFFA cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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