SPICE1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPICE1
Entrez Gene ID	152185
Full Name	spindle and centriole associated protein 1
Synonyms	CCDC52,SPICE
General protein information	Preferred Names spindle and centriole associated protein 1 Names spindle and centriole-associated protein 1 coiled-coil domain-containing protein 52
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q13.2
Disorder MIM:	613447

mRNA and Protein(s)

mRNA	Protein	Name
XM_017005778.1	XP_016861267.1	spindle and centriole-associated protein 1 isoform X3
NM_144718.3	NP_653319.1	spindle and centriole-associated protein 1 isoform a
NM_001331078.1	NP_001318007.1	spindle and centriole-associated protein 1 isoform a
XM_017005775.1	XP_016861264.1	spindle and centriole-associated protein 1 isoform X1
NM_001331079.1	NP_001318008.1	spindle and centriole-associated protein 1 isoform b
NM_001331078.2	NP_001318007.1	spindle and centriole-associated protein 1 isoform a
NM_144718.4	NP_653319.1	spindle and centriole-associated protein 1 isoform a
XM_017005776.1	XP_016861265.1	spindle and centriole-associated protein 1 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SPICE1	NP_001033206.1
Rattus norvegicus (Norway rat)	Spice1	NP_001008286.1
Danio rerio (zebrafish)	spice1	NP_001008636.1
	SPICE1	XP_001106381.1
Canis lupus familiaris (dog)	SPICE1	XP_535744.3
Mus musculus (house mouse)	Spice1	NP_653133.3
Gallus gallus (chicken)	SPICE1	XP_416545.4
Homo sapiens (human)	SPICE1	NP_653319.1
Pan troglodytes (chimpanzee)	SPICE1	XP_001158122.1
Xenopus tropicalis (tropical clawed frog)	spice1	NP_001121472.1

Gene Ontology
Bibliography

Related articles in PubMed

A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface.
Gupta GD, Coyaud ?, Gon?alves J, Mojarad BA, Liu Y, Wu Q, Gheiratmand L, Comartin D, Tkach JM, Cheung SW, Bashkurov M, Hasegan M, Knight JD, Lin ZY, Schueler M, Hildebrandt F, Moffat J, Gingras AC, Raught B, Pelletier L
Cell163(6)1484-99(2015 Dec)

CEP120 and SPICE1 cooperate with CPAP in centriole elongation.
Comartin D, Gupta GD, Fussner E, Coyaud ?, Hasegan M, Archinti M, Cheung SW, Pinchev D, Lawo S, Raught B, Bazett-Jones DP, L?ders J, Pelletier L
Current biology : CB23(14)1360-6(2013 Jul)

A Y2H-seq approach defines the human protein methyltransferase interactome.
Weimann M, Grossmann A, Woodsmith J, ?zkan Z, Birth P, Meierhofer D, Benlasfer N, Valovka T, Timmermann B, Wanker EE, Sauer S, Stelzl U
Nature methods10(4)339-42(2013 Apr)

Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods.
Jakobsen L, Vanselow K, Skogs M, Toyoda Y, Lundberg E, Poser I, Falkenby LG, Bennetzen M, Westendorf J, Nigg EA, Uhlen M, Hyman AA, Andersen JS
The EMBO journal30(8)1520-35(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CEP120 associates with SPICE1 and CPAP, and depletion of any of these proteins results in short procentrioles. Furthermore, CEP120 or CPAP overexpression results in excessive centriole elongation, a process dependent on CEP120, SPICE1, and CPAP.
Title: CEP120 and SPICE1 cooperate with CPAP in centriole elongation.

SPICE is required for centriole duplication and mitotic chromosome congression.
Title: SPICE--a previously uncharacterized protein required for centriole duplication and mitotic chromosome congression.

The following SPICE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPICE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13732	NM_001331078.2 Latest version!	Homo sapiens spindle and centriole associated protein 1 (SPICE1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OHu13732	NM_144718.4 Latest version!	Homo sapiens spindle and centriole associated protein 1 (SPICE1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OHu13732	XM_017005778.1 Latest version!	Homo sapiens spindle and centriole associated protein 1 (SPICE1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OHu76713	NM_001331079.1 Latest version!	Homo sapiens spindle and centriole associated protein 1 (SPICE1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu76713	XM_017005775.1 Latest version!	Homo sapiens spindle and centriole associated protein 1 (SPICE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu93840	XM_017005776.1 Latest version!	Homo sapiens spindle and centriole associated protein 1 (SPICE1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OHu13732	NM_001331078.1	Homo sapiens spindle and centriole associated protein 1 (SPICE1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OHu13732	NM_144718.3	Homo sapiens spindle and centriole associated protein 1 (SPICE1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPICE1 ( NM_144718.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_653319.1 Homo sapiens spindle and centriole associated protein 1 (SPICE1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu13732
Clone ID Related Accession (Same CDS sequence)	XM_017005778.1 , NM_144718.3 , NM_001331078.1 , NM_001331078.2 , NM_144718.4
Accession Version	NM_144718.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	spindle and centriole-associated protein 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP278430.1, AY099107.1, AC112128.4 and U79249.1. On Jun 28, 2008 this sequence version replaced NM_144718.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the shorter isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AL832148.1, SRR1803614.116530.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCATTTG TCAGAGTGAA CCGCTGTGGT CCCCGAGTTG GTGTAAGAAA GACACCGAAA 
GTAAAGAAGA AGAAAACTTC AGTGAAACAA GAATGGGATA ATACCGTGAC TGATCTAACC 
GTTCATCGGG CAACTCCCGA AGATCTGGTA CGCCGTCATG AAATACACAA ATCGAAGAAT 
AGAGCATTAG TACACTGGGA ACTCCAAGAA AAAGCTTTGA AGAGAAAATG GAGGAAGCAG 
AAACCAGAAA CTTTAAATCT TGAGAAAAGA AGATTGTCTA TCATGAAGGA GATTCTTTCT 
GATCAATACC AGATGCAAGA TGTGTTGGAG AAATCTGATC ATCTAATAGC TGCAGCAAAA 
GAGCTGTTTC CTCGTAGGCG CACAGGGTTT CCAAATGTAA CAGTGGCTCC TGATTCCTCT 
CAGGGTCCCA TTGTGGTAAA TCAAGACCCT ATCACCCAAT CTATCTTTAA TGAGTCTGTC 
ATAGAACCTC AGGCTCTTAA TGATGTAGAT GGTGAAGAAG AAGGAACTGT TAATAGCCAG 
TCAGGAGAAA GTGAGAATGA GAATGAGTTG GATAACTCTC TAAACTCTCA GTCTAACACG 
AATACAGACA GGTTTCTCCA ACAACTAACA GAAGAGAATT TTGAGTTAAT TAGTAAGTTG 
TGGACTGACA TTCAGCAGAA AATAGCAACC CAGTCACAAA TAACTCCTCC AGGAACGCCA 
TCATCTGCTC TTTCATCAGG GGAGCAAAGA GCTGCTCTGA ATGCTACCAA TGCTGTCAAG 
AGACTCCAAA CCAGGCTTCA GCCTGAAGAA TCTACTGAGA CTCTAGACTC AAGCTACGTT 
GTGGGACACG TGCTGAACTC AAGGAAGCAA AAACAGCTGT TAAATAAAGT GAAAAGGAAA 
CCGAATTTGC ATGCTCTTTC CAAGCCGAAG AAAAACATAT CATCAGGTAG CACAACCTCT 
GCAGACTTAC CAAATAGGAC TAATTCCAAC CTGGATGTCC TCAAACACAT GATACATGAA 
GTGGAACATG AAATGGAAGA ATATGAGCGG TGGACAGGTC GCGAGGTCAA GGGTCTGCAG 
AGCAGTCAGG GTCTTACAGG CTTCACTTTG TCGCTGGTGA GCTCCCTCTG TCGCCTGGTT 
CGGTACCTTA AAGAGAGTGA GATCCAGCTA CGTAAAGAAG TAGAGACAAG GCAACAACTG 
GAACAAGTAT TAGGTGATCA TCGAGAGCTC ATTGATGCTC TGACAGCTGA AATTCTTCGT 
CTTAGAGAAG AAAACGCTGC TACACAGGCA AGACTTCAGC AGTACATGGT CACAACAGAT 
GAGCAACTGA TATCACTCAC ACATGCTATT AAGAACTGTC CTGTGATAAA TAACAGACAA 
GAAATTCAGG CATCAGAAAG CGGAGCCACA GGTAGAAGAG TTATGGACAG TCCAGAGCGT 
CCAGTTGTAA ATGCCAATGT CTCAGTGCCA TTGATGTTCA GAGAGGAAGT GGCTGAATTC 
CCACAGGAAG AGTTGCCCGT TAAACTGTCT CAGGTGCCAG ACCCTCCAGA TAACATGAAT 
CTGGCCAAGA ATTTTCCAGC ACATATTTTT GAGCCAGCTG TGTTGTTAAC ACCACCCAGG 
CAGAAGAGCA ACTTAAAATT CTCTCCTCTT CAGGACGTAT TGAGAAGGAC TGTTCAAACT 
CGTCCTGCTC CACGACTTCC TCCAACTGTG GAAATAATTG AGAAGGAACA AAATTGGGAA 
GAGAAGACCT TACCTATTGA TACAGACATT CAGAATTCAA GTGAAGAGAA TCGTCTCTTC 
ACTCAGAGAT GGAGAGTCTC TCACATGGGA GAAGATTTGG AGAACAAAAC TCAGGCTCCT 
TTTGTTAACC TCTCACAGCC CCTCTGCAAT TCCCATTCCA ACACTCAACA GTCAAGAAGC 
CCCACATTCT CAGAAGAGCT CCCAGTACTG GGAGATGGGC AGCAGCTGAG AACAAATGAG 
TCATTAATAC AAAGAAAGGA CATAATGACA CGAATTGCTG ATTTGACATT GCAGAATTCA 
GCTATCAAGG CACATATGAA TAATATTATT GAGCCCAGAG GAGAGCAAGG GGATGGACTC 
CGGGAGTTGA ACAAACAAGA AAGTGCAAGT GACATGACTT CTACTTTTCC AGTAGCACAG 
TCTCTAACAC CAGGTAGTAT GGAGGAACGG ATTGCAGAAT TGAATCGACA AAGTATGGAG 
GCTCGTGGAA AACTACTGCA GTTGATAGAG CAGCAGAAAC TTGTTGGTTT GAATCTTTCT 
CCACCAATGT CACCTGTTCA GTTACCTCTC AGAGCATGGA CTGAAGGAGC AAAGAGGACA 
ATTGAGGTAT CTATTCCAGG AGCAGAAGCC CCAGAAAGCT CAAAATGTAG TACTGTCTCT 
CCCGTCAGCG GGATAAATAC AAGAAGATCT TCCGGGGCTA CTGGTAATTC TTGTTCTCCA 
CTAAATGCCA CCTCAGGAAG TGGGAGATTC ACACCTCTTA ATCCAAGAGC AAAGATTGAG 
AAACAGAATG AAGAAGGCTG GTTTGCTCTT TCTACCCATG TATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_653319.1
CDS	292..2859
Translation	MSFVRVNRCGPRVGVRKTPKVKKKKTSVKQEWDNTVTDLTVHRATPEDLVRRHEIHKSKNRALVHWELQEKALKRKWRKQKPETLNLEKRRLSIMKEILSDQYQMQDVLEKSDHLIAAAKELFPRRRTGFPNVTVAPDSSQGPIVVNQDPITQSIFNESVIEPQALNDVDGEEEGTVNSQSGESENENELDNSLNSQSNTNTDRFLQQLTEENFELISKLWTDIQQKIATQSQITPPGTPSSALSSGEQRAALNATNAVKRLQTRLQPEESTETLDSSYVVGHVLNSRKQKQLLNKVKRKPNLHALSKPKKNISSGSTTSADLPNRTNSNLDVLKHMIHEVEHEMEEYERWTGREVKGLQSSQGLTGFTLSLVSSLCRLVRYLKESEIQLRKEVETRQQLEQVLGDHRELIDALTAEILRLREENAATQARLQQYMVTTDEQLISLTHAIKNCPVINNRQEIQASESGATGRRVMDSPERPVVNANVSVPLMFREEVAEFPQEELPVKLSQVPDPPDNMNLAKNFPAHIFEPAVLLTPPRQKSNLKFSPLQDVLRRTVQTRPAPRLPPTVEIIEKEQNWEEKTLPIDTDIQNSSEENRLFTQRWRVSHMGEDLENKTQAPFVNLSQPLCNSHSNTQQSRSPTFSEELPVLGDGQQLRTNESLIQRKDIMTRIADLTLQNSAIKAHMNNIIEPRGEQGDGLRELNKQESASDMTSTFPVAQSLTPGSMEERIAELNRQSMEARGKLLQLIEQQKLVGLNLSPPMSPVQLPLRAWTEGAKRTIEVSIPGAEAPESSKCSTVSPVSGINTRRSSGATGNSCSPLNATSGSGRFTPLNPRAKIEKQNEEGWFALSTHVS

Target ORF information:

RefSeq Version	NM_144718.3
Organism	Homo sapiens(human)
Definition	Homo sapiens spindle and centriole associated protein 1 (SPICE1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144718.3

ORF Insert Sequence:

ATGTCATTTG TCAGAGTGAA CCGCTGTGGT CCCCGAGTTG GTGTAAGAAA GACACCGAAA 
GTAAAGAAGA AGAAAACTTC AGTGAAACAA GAATGGGATA ATACCGTGAC TGATCTAACC 
GTTCATCGGG CAACTCCCGA AGATCTGGTA CGCCGTCATG AAATACACAA ATCGAAGAAT 
AGAGCATTAG TACACTGGGA ACTCCAAGAA AAAGCTTTGA AGAGAAAATG GAGGAAGCAG 
AAACCAGAAA CTTTAAATCT TGAGAAAAGA AGATTGTCTA TCATGAAGGA GATTCTTTCT 
GATCAATACC AGATGCAAGA TGTGTTGGAG AAATCTGATC ATCTAATAGC TGCAGCAAAA 
GAGCTGTTTC CTCGTAGGCG CACAGGGTTT CCAAATGTAA CAGTGGCTCC TGATTCCTCT 
CAGGGTCCCA TTGTGGTAAA TCAAGACCCT ATCACCCAAT CTATCTTTAA TGAGTCTGTC 
ATAGAACCTC AGGCTCTTAA TGATGTAGAT GGTGAAGAAG AAGGAACTGT TAATAGCCAG 
TCAGGAGAAA GTGAGAATGA GAATGAGTTG GATAACTCTC TAAACTCTCA GTCTAACACG 
AATACAGACA GGTTTCTCCA ACAACTAACA GAAGAGAATT TTGAGTTAAT TAGTAAGTTG 
TGGACTGACA TTCAGCAGAA AATAGCAACC CAGTCACAAA TAACTCCTCC AGGAACGCCA 
TCATCTGCTC TTTCATCAGG GGAGCAAAGA GCTGCTCTGA ATGCTACCAA TGCTGTCAAG 
AGACTCCAAA CCAGGCTTCA GCCTGAAGAA TCTACTGAGA CTCTAGACTC AAGCTACGTT 
GTGGGACACG TGCTGAACTC AAGGAAGCAA AAACAGCTGT TAAATAAAGT GAAAAGGAAA 
CCGAATTTGC ATGCTCTTTC CAAGCCGAAG AAAAACATAT CATCAGGTAG CACAACCTCT 
GCAGACTTAC CAAATAGGAC TAATTCCAAC CTGGATGTCC TCAAACACAT GATACATGAA 
GTGGAACATG AAATGGAAGA ATATGAGCGG TGGACAGGTC GCGAGGTCAA GGGTCTGCAG 
AGCAGTCAGG GTCTTACAGG CTTCACTTTG TCGCTGGTGA GCTCCCTCTG TCGCCTGGTT 
CGGTACCTTA AAGAGAGTGA GATCCAGCTA CGTAAAGAAG TAGAGACAAG GCAACAACTG 
GAACAAGTAT TAGGTGATCA TCGAGAGCTC ATTGATGCTC TGACAGCTGA AATTCTTCGT 
CTTAGAGAAG AAAACGCTGC TACACAGGCA AGACTTCAGC AGTACATGGT CACAACAGAT 
GAGCAACTGA TATCACTCAC ACATGCTATT AAGAACTGTC CTGTGATAAA TAACAGACAA 
GAAATTCAGG CATCAGAAAG CGGAGCCACA GGTAGAAGAG TTATGGACAG TCCAGAGCGT 
CCAGTTGTAA ATGCCAATGT CTCAGTGCCA TTGATGTTCA GAGAGGAAGT GGCTGAATTC 
CCACAGGAAG AGTTGCCCGT TAAACTGTCT CAGGTGCCAG ACCCTCCAGA TAACATGAAT 
CTGGCCAAGA ATTTTCCAGC ACATATTTTT GAGCCAGCTG TGTTGTTAAC ACCACCCAGG 
CAGAAGAGCA ACTTAAAATT CTCTCCTCTT CAGGACGTAT TGAGAAGGAC TGTTCAAACT 
CGTCCTGCTC CACGACTTCC TCCAACTGTG GAAATAATTG AGAAGGAACA AAATTGGGAA 
GAGAAGACCT TACCTATTGA TACAGACATT CAGAATTCAA GTGAAGAGAA TCGTCTCTTC 
ACTCAGAGAT GGAGAGTCTC TCACATGGGA GAAGATTTGG AGAACAAAAC TCAGGCTCCT 
TTTGTTAACC TCTCACAGCC CCTCTGCAAT TCCCATTCCA ACACTCAACA GTCAAGAAGC 
CCCACATTCT CAGAAGAGCT CCCAGTACTG GGAGATGGGC AGCAGCTGAG AACAAATGAG 
TCATTAATAC AAAGAAAGGA CATAATGACA CGAATTGCTG ATTTGACATT GCAGAATTCA 
GCTATCAAGG CACATATGAA TAATATTATT GAGCCCAGAG GAGAGCAAGG GGATGGACTC 
CGGGAGTTGA ACAAACAAGA AAGTGCAAGT GACATGACTT CTACTTTTCC AGTAGCACAG 
TCTCTAACAC CAGGTAGTAT GGAGGAACGG ATTGCAGAAT TGAATCGACA AAGTATGGAG 
GCTCGTGGAA AACTACTGCA GTTGATAGAG CAGCAGAAAC TTGTTGGTTT GAATCTTTCT 
CCACCAATGT CACCTGTTCA GTTACCTCTC AGAGCATGGA CTGAAGGAGC AAAGAGGACA 
ATTGAGGTAT CTATTCCAGG AGCAGAAGCC CCAGAAAGCT CAAAATGTAG TACTGTCTCT 
CCCGTCAGCG GGATAAATAC AAGAAGATCT TCCGGGGCTA CTGGTAATTC TTGTTCTCCA 
CTAAATGCCA CCTCAGGAAG TGGGAGATTC ACACCTCTTA ATCCAAGAGC AAAGATTGAG 
AAACAGAATG AAGAAGGCTG GTTTGCTCTT TCTACCCATG TATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPICE1 ( NM_001331078.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001318007.1 Homo sapiens spindle and centriole associated protein 1 (SPICE1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu13732
Clone ID Related Accession (Same CDS sequence)	XM_017005778.1 , NM_144718.3 , NM_001331078.1 , NM_001331078.2 , NM_144718.4
Accession Version	NM_001331078.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	spindle and centriole-associated protein 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC112128.4. On Sep 8, 2016 this sequence version replaced XM_017005777.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.109863.1, SRR1803617.62446.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCATTTG TCAGAGTGAA CCGCTGTGGT CCCCGAGTTG GTGTAAGAAA GACACCGAAA 
GTAAAGAAGA AGAAAACTTC AGTGAAACAA GAATGGGATA ATACCGTGAC TGATCTAACC 
GTTCATCGGG CAACTCCCGA AGATCTGGTA CGCCGTCATG AAATACACAA ATCGAAGAAT 
AGAGCATTAG TACACTGGGA ACTCCAAGAA AAAGCTTTGA AGAGAAAATG GAGGAAGCAG 
AAACCAGAAA CTTTAAATCT TGAGAAAAGA AGATTGTCTA TCATGAAGGA GATTCTTTCT 
GATCAATACC AGATGCAAGA TGTGTTGGAG AAATCTGATC ATCTAATAGC TGCAGCAAAA 
GAGCTGTTTC CTCGTAGGCG CACAGGGTTT CCAAATGTAA CAGTGGCTCC TGATTCCTCT 
CAGGGTCCCA TTGTGGTAAA TCAAGACCCT ATCACCCAAT CTATCTTTAA TGAGTCTGTC 
ATAGAACCTC AGGCTCTTAA TGATGTAGAT GGTGAAGAAG AAGGAACTGT TAATAGCCAG 
TCAGGAGAAA GTGAGAATGA GAATGAGTTG GATAACTCTC TAAACTCTCA GTCTAACACG 
AATACAGACA GGTTTCTCCA ACAACTAACA GAAGAGAATT TTGAGTTAAT TAGTAAGTTG 
TGGACTGACA TTCAGCAGAA AATAGCAACC CAGTCACAAA TAACTCCTCC AGGAACGCCA 
TCATCTGCTC TTTCATCAGG GGAGCAAAGA GCTGCTCTGA ATGCTACCAA TGCTGTCAAG 
AGACTCCAAA CCAGGCTTCA GCCTGAAGAA TCTACTGAGA CTCTAGACTC AAGCTACGTT 
GTGGGACACG TGCTGAACTC AAGGAAGCAA AAACAGCTGT TAAATAAAGT GAAAAGGAAA 
CCGAATTTGC ATGCTCTTTC CAAGCCGAAG AAAAACATAT CATCAGGTAG CACAACCTCT 
GCAGACTTAC CAAATAGGAC TAATTCCAAC CTGGATGTCC TCAAACACAT GATACATGAA 
GTGGAACATG AAATGGAAGA ATATGAGCGG TGGACAGGTC GCGAGGTCAA GGGTCTGCAG 
AGCAGTCAGG GTCTTACAGG CTTCACTTTG TCGCTGGTGA GCTCCCTCTG TCGCCTGGTT 
CGGTACCTTA AAGAGAGTGA GATCCAGCTA CGTAAAGAAG TAGAGACAAG GCAACAACTG 
GAACAAGTAT TAGGTGATCA TCGAGAGCTC ATTGATGCTC TGACAGCTGA AATTCTTCGT 
CTTAGAGAAG AAAACGCTGC TACACAGGCA AGACTTCAGC AGTACATGGT CACAACAGAT 
GAGCAACTGA TATCACTCAC ACATGCTATT AAGAACTGTC CTGTGATAAA TAACAGACAA 
GAAATTCAGG CATCAGAAAG CGGAGCCACA GGTAGAAGAG TTATGGACAG TCCAGAGCGT 
CCAGTTGTAA ATGCCAATGT CTCAGTGCCA TTGATGTTCA GAGAGGAAGT GGCTGAATTC 
CCACAGGAAG AGTTGCCCGT TAAACTGTCT CAGGTGCCAG ACCCTCCAGA TAACATGAAT 
CTGGCCAAGA ATTTTCCAGC ACATATTTTT GAGCCAGCTG TGTTGTTAAC ACCACCCAGG 
CAGAAGAGCA ACTTAAAATT CTCTCCTCTT CAGGACGTAT TGAGAAGGAC TGTTCAAACT 
CGTCCTGCTC CACGACTTCC TCCAACTGTG GAAATAATTG AGAAGGAACA AAATTGGGAA 
GAGAAGACCT TACCTATTGA TACAGACATT CAGAATTCAA GTGAAGAGAA TCGTCTCTTC 
ACTCAGAGAT GGAGAGTCTC TCACATGGGA GAAGATTTGG AGAACAAAAC TCAGGCTCCT 
TTTGTTAACC TCTCACAGCC CCTCTGCAAT TCCCATTCCA ACACTCAACA GTCAAGAAGC 
CCCACATTCT CAGAAGAGCT CCCAGTACTG GGAGATGGGC AGCAGCTGAG AACAAATGAG 
TCATTAATAC AAAGAAAGGA CATAATGACA CGAATTGCTG ATTTGACATT GCAGAATTCA 
GCTATCAAGG CACATATGAA TAATATTATT GAGCCCAGAG GAGAGCAAGG GGATGGACTC 
CGGGAGTTGA ACAAACAAGA AAGTGCAAGT GACATGACTT CTACTTTTCC AGTAGCACAG 
TCTCTAACAC CAGGTAGTAT GGAGGAACGG ATTGCAGAAT TGAATCGACA AAGTATGGAG 
GCTCGTGGAA AACTACTGCA GTTGATAGAG CAGCAGAAAC TTGTTGGTTT GAATCTTTCT 
CCACCAATGT CACCTGTTCA GTTACCTCTC AGAGCATGGA CTGAAGGAGC AAAGAGGACA 
ATTGAGGTAT CTATTCCAGG AGCAGAAGCC CCAGAAAGCT CAAAATGTAG TACTGTCTCT 
CCCGTCAGCG GGATAAATAC AAGAAGATCT TCCGGGGCTA CTGGTAATTC TTGTTCTCCA 
CTAAATGCCA CCTCAGGAAG TGGGAGATTC ACACCTCTTA ATCCAAGAGC AAAGATTGAG 
AAACAGAATG AAGAAGGCTG GTTTGCTCTT TCTACCCATG TATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001318007.1
CDS	109..2676
Translation	MSFVRVNRCGPRVGVRKTPKVKKKKTSVKQEWDNTVTDLTVHRATPEDLVRRHEIHKSKNRALVHWELQEKALKRKWRKQKPETLNLEKRRLSIMKEILSDQYQMQDVLEKSDHLIAAAKELFPRRRTGFPNVTVAPDSSQGPIVVNQDPITQSIFNESVIEPQALNDVDGEEEGTVNSQSGESENENELDNSLNSQSNTNTDRFLQQLTEENFELISKLWTDIQQKIATQSQITPPGTPSSALSSGEQRAALNATNAVKRLQTRLQPEESTETLDSSYVVGHVLNSRKQKQLLNKVKRKPNLHALSKPKKNISSGSTTSADLPNRTNSNLDVLKHMIHEVEHEMEEYERWTGREVKGLQSSQGLTGFTLSLVSSLCRLVRYLKESEIQLRKEVETRQQLEQVLGDHRELIDALTAEILRLREENAATQARLQQYMVTTDEQLISLTHAIKNCPVINNRQEIQASESGATGRRVMDSPERPVVNANVSVPLMFREEVAEFPQEELPVKLSQVPDPPDNMNLAKNFPAHIFEPAVLLTPPRQKSNLKFSPLQDVLRRTVQTRPAPRLPPTVEIIEKEQNWEEKTLPIDTDIQNSSEENRLFTQRWRVSHMGEDLENKTQAPFVNLSQPLCNSHSNTQQSRSPTFSEELPVLGDGQQLRTNESLIQRKDIMTRIADLTLQNSAIKAHMNNIIEPRGEQGDGLRELNKQESASDMTSTFPVAQSLTPGSMEERIAELNRQSMEARGKLLQLIEQQKLVGLNLSPPMSPVQLPLRAWTEGAKRTIEVSIPGAEAPESSKCSTVSPVSGINTRRSSGATGNSCSPLNATSGSGRFTPLNPRAKIEKQNEEGWFALSTHVS

Target ORF information:

RefSeq Version	NM_001331078.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spindle and centriole associated protein 1 (SPICE1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331078.1

ORF Insert Sequence:

ATGTCATTTG TCAGAGTGAA CCGCTGTGGT CCCCGAGTTG GTGTAAGAAA GACACCGAAA 
GTAAAGAAGA AGAAAACTTC AGTGAAACAA GAATGGGATA ATACCGTGAC TGATCTAACC 
GTTCATCGGG CAACTCCCGA AGATCTGGTA CGCCGTCATG AAATACACAA ATCGAAGAAT 
AGAGCATTAG TACACTGGGA ACTCCAAGAA AAAGCTTTGA AGAGAAAATG GAGGAAGCAG 
AAACCAGAAA CTTTAAATCT TGAGAAAAGA AGATTGTCTA TCATGAAGGA GATTCTTTCT 
GATCAATACC AGATGCAAGA TGTGTTGGAG AAATCTGATC ATCTAATAGC TGCAGCAAAA 
GAGCTGTTTC CTCGTAGGCG CACAGGGTTT CCAAATGTAA CAGTGGCTCC TGATTCCTCT 
CAGGGTCCCA TTGTGGTAAA TCAAGACCCT ATCACCCAAT CTATCTTTAA TGAGTCTGTC 
ATAGAACCTC AGGCTCTTAA TGATGTAGAT GGTGAAGAAG AAGGAACTGT TAATAGCCAG 
TCAGGAGAAA GTGAGAATGA GAATGAGTTG GATAACTCTC TAAACTCTCA GTCTAACACG 
AATACAGACA GGTTTCTCCA ACAACTAACA GAAGAGAATT TTGAGTTAAT TAGTAAGTTG 
TGGACTGACA TTCAGCAGAA AATAGCAACC CAGTCACAAA TAACTCCTCC AGGAACGCCA 
TCATCTGCTC TTTCATCAGG GGAGCAAAGA GCTGCTCTGA ATGCTACCAA TGCTGTCAAG 
AGACTCCAAA CCAGGCTTCA GCCTGAAGAA TCTACTGAGA CTCTAGACTC AAGCTACGTT 
GTGGGACACG TGCTGAACTC AAGGAAGCAA AAACAGCTGT TAAATAAAGT GAAAAGGAAA 
CCGAATTTGC ATGCTCTTTC CAAGCCGAAG AAAAACATAT CATCAGGTAG CACAACCTCT 
GCAGACTTAC CAAATAGGAC TAATTCCAAC CTGGATGTCC TCAAACACAT GATACATGAA 
GTGGAACATG AAATGGAAGA ATATGAGCGG TGGACAGGTC GCGAGGTCAA GGGTCTGCAG 
AGCAGTCAGG GTCTTACAGG CTTCACTTTG TCGCTGGTGA GCTCCCTCTG TCGCCTGGTT 
CGGTACCTTA AAGAGAGTGA GATCCAGCTA CGTAAAGAAG TAGAGACAAG GCAACAACTG 
GAACAAGTAT TAGGTGATCA TCGAGAGCTC ATTGATGCTC TGACAGCTGA AATTCTTCGT 
CTTAGAGAAG AAAACGCTGC TACACAGGCA AGACTTCAGC AGTACATGGT CACAACAGAT 
GAGCAACTGA TATCACTCAC ACATGCTATT AAGAACTGTC CTGTGATAAA TAACAGACAA 
GAAATTCAGG CATCAGAAAG CGGAGCCACA GGTAGAAGAG TTATGGACAG TCCAGAGCGT 
CCAGTTGTAA ATGCCAATGT CTCAGTGCCA TTGATGTTCA GAGAGGAAGT GGCTGAATTC 
CCACAGGAAG AGTTGCCCGT TAAACTGTCT CAGGTGCCAG ACCCTCCAGA TAACATGAAT 
CTGGCCAAGA ATTTTCCAGC ACATATTTTT GAGCCAGCTG TGTTGTTAAC ACCACCCAGG 
CAGAAGAGCA ACTTAAAATT CTCTCCTCTT CAGGACGTAT TGAGAAGGAC TGTTCAAACT 
CGTCCTGCTC CACGACTTCC TCCAACTGTG GAAATAATTG AGAAGGAACA AAATTGGGAA 
GAGAAGACCT TACCTATTGA TACAGACATT CAGAATTCAA GTGAAGAGAA TCGTCTCTTC 
ACTCAGAGAT GGAGAGTCTC TCACATGGGA GAAGATTTGG AGAACAAAAC TCAGGCTCCT 
TTTGTTAACC TCTCACAGCC CCTCTGCAAT TCCCATTCCA ACACTCAACA GTCAAGAAGC 
CCCACATTCT CAGAAGAGCT CCCAGTACTG GGAGATGGGC AGCAGCTGAG AACAAATGAG 
TCATTAATAC AAAGAAAGGA CATAATGACA CGAATTGCTG ATTTGACATT GCAGAATTCA 
GCTATCAAGG CACATATGAA TAATATTATT GAGCCCAGAG GAGAGCAAGG GGATGGACTC 
CGGGAGTTGA ACAAACAAGA AAGTGCAAGT GACATGACTT CTACTTTTCC AGTAGCACAG 
TCTCTAACAC CAGGTAGTAT GGAGGAACGG ATTGCAGAAT TGAATCGACA AAGTATGGAG 
GCTCGTGGAA AACTACTGCA GTTGATAGAG CAGCAGAAAC TTGTTGGTTT GAATCTTTCT 
CCACCAATGT CACCTGTTCA GTTACCTCTC AGAGCATGGA CTGAAGGAGC AAAGAGGACA 
ATTGAGGTAT CTATTCCAGG AGCAGAAGCC CCAGAAAGCT CAAAATGTAG TACTGTCTCT 
CCCGTCAGCG GGATAAATAC AAGAAGATCT TCCGGGGCTA CTGGTAATTC TTGTTCTCCA 
CTAAATGCCA CCTCAGGAAG TGGGAGATTC ACACCTCTTA ATCCAAGAGC AAAGATTGAG 
AAACAGAATG AAGAAGGCTG GTTTGCTCTT TCTACCCATG TATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPICE1 ( NM_001331078.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001318007.1 Homo sapiens spindle and centriole associated protein 1 (SPICE1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu13732
Clone ID Related Accession (Same CDS sequence)	XM_017005778.1 , NM_144718.3 , NM_001331078.1 , NM_001331078.2 , NM_144718.4
Accession Version	NM_001331078.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Homo sapiens(human)
Product	spindle and centriole-associated protein 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC112128.4. On Jun 1, 2019 this sequence version replaced NM_001331078.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.62446.1, SRR1803616.16438.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCATTTG TCAGAGTGAA CCGCTGTGGT CCCCGAGTTG GTGTAAGAAA GACACCGAAA 
GTAAAGAAGA AGAAAACTTC AGTGAAACAA GAATGGGATA ATACCGTGAC TGATCTAACC 
GTTCATCGGG CAACTCCCGA AGATCTGGTA CGCCGTCATG AAATACACAA ATCGAAGAAT 
AGAGCATTAG TACACTGGGA ACTCCAAGAA AAAGCTTTGA AGAGAAAATG GAGGAAGCAG 
AAACCAGAAA CTTTAAATCT TGAGAAAAGA AGATTGTCTA TCATGAAGGA GATTCTTTCT 
GATCAATACC AGATGCAAGA TGTGTTGGAG AAATCTGATC ATCTAATAGC TGCAGCAAAA 
GAGCTGTTTC CTCGTAGGCG CACAGGGTTT CCAAATGTAA CAGTGGCTCC TGATTCCTCT 
CAGGGTCCCA TTGTGGTAAA TCAAGACCCT ATCACCCAAT CTATCTTTAA TGAGTCTGTC 
ATAGAACCTC AGGCTCTTAA TGATGTAGAT GGTGAAGAAG AAGGAACTGT TAATAGCCAG 
TCAGGAGAAA GTGAGAATGA GAATGAGTTG GATAACTCTC TAAACTCTCA GTCTAACACG 
AATACAGACA GGTTTCTCCA ACAACTAACA GAAGAGAATT TTGAGTTAAT TAGTAAGTTG 
TGGACTGACA TTCAGCAGAA AATAGCAACC CAGTCACAAA TAACTCCTCC AGGAACGCCA 
TCATCTGCTC TTTCATCAGG GGAGCAAAGA GCTGCTCTGA ATGCTACCAA TGCTGTCAAG 
AGACTCCAAA CCAGGCTTCA GCCTGAAGAA TCTACTGAGA CTCTAGACTC AAGCTACGTT 
GTGGGACACG TGCTGAACTC AAGGAAGCAA AAACAGCTGT TAAATAAAGT GAAAAGGAAA 
CCGAATTTGC ATGCTCTTTC CAAGCCGAAG AAAAACATAT CATCAGGTAG CACAACCTCT 
GCAGACTTAC CAAATAGGAC TAATTCCAAC CTGGATGTCC TCAAACACAT GATACATGAA 
GTGGAACATG AAATGGAAGA ATATGAGCGG TGGACAGGTC GCGAGGTCAA GGGTCTGCAG 
AGCAGTCAGG GTCTTACAGG CTTCACTTTG TCGCTGGTGA GCTCCCTCTG TCGCCTGGTT 
CGGTACCTTA AAGAGAGTGA GATCCAGCTA CGTAAAGAAG TAGAGACAAG GCAACAACTG 
GAACAAGTAT TAGGTGATCA TCGAGAGCTC ATTGATGCTC TGACAGCTGA AATTCTTCGT 
CTTAGAGAAG AAAACGCTGC TACACAGGCA AGACTTCAGC AGTACATGGT CACAACAGAT 
GAGCAACTGA TATCACTCAC ACATGCTATT AAGAACTGTC CTGTGATAAA TAACAGACAA 
GAAATTCAGG CATCAGAAAG CGGAGCCACA GGTAGAAGAG TTATGGACAG TCCAGAGCGT 
CCAGTTGTAA ATGCCAATGT CTCAGTGCCA TTGATGTTCA GAGAGGAAGT GGCTGAATTC 
CCACAGGAAG AGTTGCCCGT TAAACTGTCT CAGGTGCCAG ACCCTCCAGA TAACATGAAT 
CTGGCCAAGA ATTTTCCAGC ACATATTTTT GAGCCAGCTG TGTTGTTAAC ACCACCCAGG 
CAGAAGAGCA ACTTAAAATT CTCTCCTCTT CAGGACGTAT TGAGAAGGAC TGTTCAAACT 
CGTCCTGCTC CACGACTTCC TCCAACTGTG GAAATAATTG AGAAGGAACA AAATTGGGAA 
GAGAAGACCT TACCTATTGA TACAGACATT CAGAATTCAA GTGAAGAGAA TCGTCTCTTC 
ACTCAGAGAT GGAGAGTCTC TCACATGGGA GAAGATTTGG AGAACAAAAC TCAGGCTCCT 
TTTGTTAACC TCTCACAGCC CCTCTGCAAT TCCCATTCCA ACACTCAACA GTCAAGAAGC 
CCCACATTCT CAGAAGAGCT CCCAGTACTG GGAGATGGGC AGCAGCTGAG AACAAATGAG 
TCATTAATAC AAAGAAAGGA CATAATGACA CGAATTGCTG ATTTGACATT GCAGAATTCA 
GCTATCAAGG CACATATGAA TAATATTATT GAGCCCAGAG GAGAGCAAGG GGATGGACTC 
CGGGAGTTGA ACAAACAAGA AAGTGCAAGT GACATGACTT CTACTTTTCC AGTAGCACAG 
TCTCTAACAC CAGGTAGTAT GGAGGAACGG ATTGCAGAAT TGAATCGACA AAGTATGGAG 
GCTCGTGGAA AACTACTGCA GTTGATAGAG CAGCAGAAAC TTGTTGGTTT GAATCTTTCT 
CCACCAATGT CACCTGTTCA GTTACCTCTC AGAGCATGGA CTGAAGGAGC AAAGAGGACA 
ATTGAGGTAT CTATTCCAGG AGCAGAAGCC CCAGAAAGCT CAAAATGTAG TACTGTCTCT 
CCCGTCAGCG GGATAAATAC AAGAAGATCT TCCGGGGCTA CTGGTAATTC TTGTTCTCCA 
CTAAATGCCA CCTCAGGAAG TGGGAGATTC ACACCTCTTA ATCCAAGAGC AAAGATTGAG 
AAACAGAATG AAGAAGGCTG GTTTGCTCTT TCTACCCATG TATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001318007.1
CDS	78..2645
Translation	MSFVRVNRCGPRVGVRKTPKVKKKKTSVKQEWDNTVTDLTVHRATPEDLVRRHEIHKSKNRALVHWELQEKALKRKWRKQKPETLNLEKRRLSIMKEILSDQYQMQDVLEKSDHLIAAAKELFPRRRTGFPNVTVAPDSSQGPIVVNQDPITQSIFNESVIEPQALNDVDGEEEGTVNSQSGESENENELDNSLNSQSNTNTDRFLQQLTEENFELISKLWTDIQQKIATQSQITPPGTPSSALSSGEQRAALNATNAVKRLQTRLQPEESTETLDSSYVVGHVLNSRKQKQLLNKVKRKPNLHALSKPKKNISSGSTTSADLPNRTNSNLDVLKHMIHEVEHEMEEYERWTGREVKGLQSSQGLTGFTLSLVSSLCRLVRYLKESEIQLRKEVETRQQLEQVLGDHRELIDALTAEILRLREENAATQARLQQYMVTTDEQLISLTHAIKNCPVINNRQEIQASESGATGRRVMDSPERPVVNANVSVPLMFREEVAEFPQEELPVKLSQVPDPPDNMNLAKNFPAHIFEPAVLLTPPRQKSNLKFSPLQDVLRRTVQTRPAPRLPPTVEIIEKEQNWEEKTLPIDTDIQNSSEENRLFTQRWRVSHMGEDLENKTQAPFVNLSQPLCNSHSNTQQSRSPTFSEELPVLGDGQQLRTNESLIQRKDIMTRIADLTLQNSAIKAHMNNIIEPRGEQGDGLRELNKQESASDMTSTFPVAQSLTPGSMEERIAELNRQSMEARGKLLQLIEQQKLVGLNLSPPMSPVQLPLRAWTEGAKRTIEVSIPGAEAPESSKCSTVSPVSGINTRRSSGATGNSCSPLNATSGSGRFTPLNPRAKIEKQNEEGWFALSTHVS

Target ORF information:

RefSeq Version	NM_001331078.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spindle and centriole associated protein 1 (SPICE1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331078.2

ORF Insert Sequence:

ATGTCATTTG TCAGAGTGAA CCGCTGTGGT CCCCGAGTTG GTGTAAGAAA GACACCGAAA 
GTAAAGAAGA AGAAAACTTC AGTGAAACAA GAATGGGATA ATACCGTGAC TGATCTAACC 
GTTCATCGGG CAACTCCCGA AGATCTGGTA CGCCGTCATG AAATACACAA ATCGAAGAAT 
AGAGCATTAG TACACTGGGA ACTCCAAGAA AAAGCTTTGA AGAGAAAATG GAGGAAGCAG 
AAACCAGAAA CTTTAAATCT TGAGAAAAGA AGATTGTCTA TCATGAAGGA GATTCTTTCT 
GATCAATACC AGATGCAAGA TGTGTTGGAG AAATCTGATC ATCTAATAGC TGCAGCAAAA 
GAGCTGTTTC CTCGTAGGCG CACAGGGTTT CCAAATGTAA CAGTGGCTCC TGATTCCTCT 
CAGGGTCCCA TTGTGGTAAA TCAAGACCCT ATCACCCAAT CTATCTTTAA TGAGTCTGTC 
ATAGAACCTC AGGCTCTTAA TGATGTAGAT GGTGAAGAAG AAGGAACTGT TAATAGCCAG 
TCAGGAGAAA GTGAGAATGA GAATGAGTTG GATAACTCTC TAAACTCTCA GTCTAACACG 
AATACAGACA GGTTTCTCCA ACAACTAACA GAAGAGAATT TTGAGTTAAT TAGTAAGTTG 
TGGACTGACA TTCAGCAGAA AATAGCAACC CAGTCACAAA TAACTCCTCC AGGAACGCCA 
TCATCTGCTC TTTCATCAGG GGAGCAAAGA GCTGCTCTGA ATGCTACCAA TGCTGTCAAG 
AGACTCCAAA CCAGGCTTCA GCCTGAAGAA TCTACTGAGA CTCTAGACTC AAGCTACGTT 
GTGGGACACG TGCTGAACTC AAGGAAGCAA AAACAGCTGT TAAATAAAGT GAAAAGGAAA 
CCGAATTTGC ATGCTCTTTC CAAGCCGAAG AAAAACATAT CATCAGGTAG CACAACCTCT 
GCAGACTTAC CAAATAGGAC TAATTCCAAC CTGGATGTCC TCAAACACAT GATACATGAA 
GTGGAACATG AAATGGAAGA ATATGAGCGG TGGACAGGTC GCGAGGTCAA GGGTCTGCAG 
AGCAGTCAGG GTCTTACAGG CTTCACTTTG TCGCTGGTGA GCTCCCTCTG TCGCCTGGTT 
CGGTACCTTA AAGAGAGTGA GATCCAGCTA CGTAAAGAAG TAGAGACAAG GCAACAACTG 
GAACAAGTAT TAGGTGATCA TCGAGAGCTC ATTGATGCTC TGACAGCTGA AATTCTTCGT 
CTTAGAGAAG AAAACGCTGC TACACAGGCA AGACTTCAGC AGTACATGGT CACAACAGAT 
GAGCAACTGA TATCACTCAC ACATGCTATT AAGAACTGTC CTGTGATAAA TAACAGACAA 
GAAATTCAGG CATCAGAAAG CGGAGCCACA GGTAGAAGAG TTATGGACAG TCCAGAGCGT 
CCAGTTGTAA ATGCCAATGT CTCAGTGCCA TTGATGTTCA GAGAGGAAGT GGCTGAATTC 
CCACAGGAAG AGTTGCCCGT TAAACTGTCT CAGGTGCCAG ACCCTCCAGA TAACATGAAT 
CTGGCCAAGA ATTTTCCAGC ACATATTTTT GAGCCAGCTG TGTTGTTAAC ACCACCCAGG 
CAGAAGAGCA ACTTAAAATT CTCTCCTCTT CAGGACGTAT TGAGAAGGAC TGTTCAAACT 
CGTCCTGCTC CACGACTTCC TCCAACTGTG GAAATAATTG AGAAGGAACA AAATTGGGAA 
GAGAAGACCT TACCTATTGA TACAGACATT CAGAATTCAA GTGAAGAGAA TCGTCTCTTC 
ACTCAGAGAT GGAGAGTCTC TCACATGGGA GAAGATTTGG AGAACAAAAC TCAGGCTCCT 
TTTGTTAACC TCTCACAGCC CCTCTGCAAT TCCCATTCCA ACACTCAACA GTCAAGAAGC 
CCCACATTCT CAGAAGAGCT CCCAGTACTG GGAGATGGGC AGCAGCTGAG AACAAATGAG 
TCATTAATAC AAAGAAAGGA CATAATGACA CGAATTGCTG ATTTGACATT GCAGAATTCA 
GCTATCAAGG CACATATGAA TAATATTATT GAGCCCAGAG GAGAGCAAGG GGATGGACTC 
CGGGAGTTGA ACAAACAAGA AAGTGCAAGT GACATGACTT CTACTTTTCC AGTAGCACAG 
TCTCTAACAC CAGGTAGTAT GGAGGAACGG ATTGCAGAAT TGAATCGACA AAGTATGGAG 
GCTCGTGGAA AACTACTGCA GTTGATAGAG CAGCAGAAAC TTGTTGGTTT GAATCTTTCT 
CCACCAATGT CACCTGTTCA GTTACCTCTC AGAGCATGGA CTGAAGGAGC AAAGAGGACA 
ATTGAGGTAT CTATTCCAGG AGCAGAAGCC CCAGAAAGCT CAAAATGTAG TACTGTCTCT 
CCCGTCAGCG GGATAAATAC AAGAAGATCT TCCGGGGCTA CTGGTAATTC TTGTTCTCCA 
CTAAATGCCA CCTCAGGAAG TGGGAGATTC ACACCTCTTA ATCCAAGAGC AAAGATTGAG 
AAACAGAATG AAGAAGGCTG GTTTGCTCTT TCTACCCATG TATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPICE1 ( NM_144718.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_653319.1 Homo sapiens spindle and centriole associated protein 1 (SPICE1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu13732
Clone ID Related Accession (Same CDS sequence)	XM_017005778.1 , NM_144718.3 , NM_001331078.1 , NM_001331078.2 , NM_144718.4
Accession Version	NM_144718.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Homo sapiens(human)
Product	spindle and centriole-associated protein 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP278430.1, AY099107.1, AC112128.4 and U79249.1. On May 17, 2019 this sequence version replaced NM_144718.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the shorter isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AL832148.1, SRR1803614.116530.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCATTTG TCAGAGTGAA CCGCTGTGGT CCCCGAGTTG GTGTAAGAAA GACACCGAAA 
GTAAAGAAGA AGAAAACTTC AGTGAAACAA GAATGGGATA ATACCGTGAC TGATCTAACC 
GTTCATCGGG CAACTCCCGA AGATCTGGTA CGCCGTCATG AAATACACAA ATCGAAGAAT 
AGAGCATTAG TACACTGGGA ACTCCAAGAA AAAGCTTTGA AGAGAAAATG GAGGAAGCAG 
AAACCAGAAA CTTTAAATCT TGAGAAAAGA AGATTGTCTA TCATGAAGGA GATTCTTTCT 
GATCAATACC AGATGCAAGA TGTGTTGGAG AAATCTGATC ATCTAATAGC TGCAGCAAAA 
GAGCTGTTTC CTCGTAGGCG CACAGGGTTT CCAAATGTAA CAGTGGCTCC TGATTCCTCT 
CAGGGTCCCA TTGTGGTAAA TCAAGACCCT ATCACCCAAT CTATCTTTAA TGAGTCTGTC 
ATAGAACCTC AGGCTCTTAA TGATGTAGAT GGTGAAGAAG AAGGAACTGT TAATAGCCAG 
TCAGGAGAAA GTGAGAATGA GAATGAGTTG GATAACTCTC TAAACTCTCA GTCTAACACG 
AATACAGACA GGTTTCTCCA ACAACTAACA GAAGAGAATT TTGAGTTAAT TAGTAAGTTG 
TGGACTGACA TTCAGCAGAA AATAGCAACC CAGTCACAAA TAACTCCTCC AGGAACGCCA 
TCATCTGCTC TTTCATCAGG GGAGCAAAGA GCTGCTCTGA ATGCTACCAA TGCTGTCAAG 
AGACTCCAAA CCAGGCTTCA GCCTGAAGAA TCTACTGAGA CTCTAGACTC AAGCTACGTT 
GTGGGACACG TGCTGAACTC AAGGAAGCAA AAACAGCTGT TAAATAAAGT GAAAAGGAAA 
CCGAATTTGC ATGCTCTTTC CAAGCCGAAG AAAAACATAT CATCAGGTAG CACAACCTCT 
GCAGACTTAC CAAATAGGAC TAATTCCAAC CTGGATGTCC TCAAACACAT GATACATGAA 
GTGGAACATG AAATGGAAGA ATATGAGCGG TGGACAGGTC GCGAGGTCAA GGGTCTGCAG 
AGCAGTCAGG GTCTTACAGG CTTCACTTTG TCGCTGGTGA GCTCCCTCTG TCGCCTGGTT 
CGGTACCTTA AAGAGAGTGA GATCCAGCTA CGTAAAGAAG TAGAGACAAG GCAACAACTG 
GAACAAGTAT TAGGTGATCA TCGAGAGCTC ATTGATGCTC TGACAGCTGA AATTCTTCGT 
CTTAGAGAAG AAAACGCTGC TACACAGGCA AGACTTCAGC AGTACATGGT CACAACAGAT 
GAGCAACTGA TATCACTCAC ACATGCTATT AAGAACTGTC CTGTGATAAA TAACAGACAA 
GAAATTCAGG CATCAGAAAG CGGAGCCACA GGTAGAAGAG TTATGGACAG TCCAGAGCGT 
CCAGTTGTAA ATGCCAATGT CTCAGTGCCA TTGATGTTCA GAGAGGAAGT GGCTGAATTC 
CCACAGGAAG AGTTGCCCGT TAAACTGTCT CAGGTGCCAG ACCCTCCAGA TAACATGAAT 
CTGGCCAAGA ATTTTCCAGC ACATATTTTT GAGCCAGCTG TGTTGTTAAC ACCACCCAGG 
CAGAAGAGCA ACTTAAAATT CTCTCCTCTT CAGGACGTAT TGAGAAGGAC TGTTCAAACT 
CGTCCTGCTC CACGACTTCC TCCAACTGTG GAAATAATTG AGAAGGAACA AAATTGGGAA 
GAGAAGACCT TACCTATTGA TACAGACATT CAGAATTCAA GTGAAGAGAA TCGTCTCTTC 
ACTCAGAGAT GGAGAGTCTC TCACATGGGA GAAGATTTGG AGAACAAAAC TCAGGCTCCT 
TTTGTTAACC TCTCACAGCC CCTCTGCAAT TCCCATTCCA ACACTCAACA GTCAAGAAGC 
CCCACATTCT CAGAAGAGCT CCCAGTACTG GGAGATGGGC AGCAGCTGAG AACAAATGAG 
TCATTAATAC AAAGAAAGGA CATAATGACA CGAATTGCTG ATTTGACATT GCAGAATTCA 
GCTATCAAGG CACATATGAA TAATATTATT GAGCCCAGAG GAGAGCAAGG GGATGGACTC 
CGGGAGTTGA ACAAACAAGA AAGTGCAAGT GACATGACTT CTACTTTTCC AGTAGCACAG 
TCTCTAACAC CAGGTAGTAT GGAGGAACGG ATTGCAGAAT TGAATCGACA AAGTATGGAG 
GCTCGTGGAA AACTACTGCA GTTGATAGAG CAGCAGAAAC TTGTTGGTTT GAATCTTTCT 
CCACCAATGT CACCTGTTCA GTTACCTCTC AGAGCATGGA CTGAAGGAGC AAAGAGGACA 
ATTGAGGTAT CTATTCCAGG AGCAGAAGCC CCAGAAAGCT CAAAATGTAG TACTGTCTCT 
CCCGTCAGCG GGATAAATAC AAGAAGATCT TCCGGGGCTA CTGGTAATTC TTGTTCTCCA 
CTAAATGCCA CCTCAGGAAG TGGGAGATTC ACACCTCTTA ATCCAAGAGC AAAGATTGAG 
AAACAGAATG AAGAAGGCTG GTTTGCTCTT TCTACCCATG TATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_653319.1
CDS	261..2828
Translation	MSFVRVNRCGPRVGVRKTPKVKKKKTSVKQEWDNTVTDLTVHRATPEDLVRRHEIHKSKNRALVHWELQEKALKRKWRKQKPETLNLEKRRLSIMKEILSDQYQMQDVLEKSDHLIAAAKELFPRRRTGFPNVTVAPDSSQGPIVVNQDPITQSIFNESVIEPQALNDVDGEEEGTVNSQSGESENENELDNSLNSQSNTNTDRFLQQLTEENFELISKLWTDIQQKIATQSQITPPGTPSSALSSGEQRAALNATNAVKRLQTRLQPEESTETLDSSYVVGHVLNSRKQKQLLNKVKRKPNLHALSKPKKNISSGSTTSADLPNRTNSNLDVLKHMIHEVEHEMEEYERWTGREVKGLQSSQGLTGFTLSLVSSLCRLVRYLKESEIQLRKEVETRQQLEQVLGDHRELIDALTAEILRLREENAATQARLQQYMVTTDEQLISLTHAIKNCPVINNRQEIQASESGATGRRVMDSPERPVVNANVSVPLMFREEVAEFPQEELPVKLSQVPDPPDNMNLAKNFPAHIFEPAVLLTPPRQKSNLKFSPLQDVLRRTVQTRPAPRLPPTVEIIEKEQNWEEKTLPIDTDIQNSSEENRLFTQRWRVSHMGEDLENKTQAPFVNLSQPLCNSHSNTQQSRSPTFSEELPVLGDGQQLRTNESLIQRKDIMTRIADLTLQNSAIKAHMNNIIEPRGEQGDGLRELNKQESASDMTSTFPVAQSLTPGSMEERIAELNRQSMEARGKLLQLIEQQKLVGLNLSPPMSPVQLPLRAWTEGAKRTIEVSIPGAEAPESSKCSTVSPVSGINTRRSSGATGNSCSPLNATSGSGRFTPLNPRAKIEKQNEEGWFALSTHVS

Target ORF information:

RefSeq Version	NM_144718.4
Organism	Homo sapiens(human)
Definition	Homo sapiens spindle and centriole associated protein 1 (SPICE1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144718.4

ORF Insert Sequence:

ATGTCATTTG TCAGAGTGAA CCGCTGTGGT CCCCGAGTTG GTGTAAGAAA GACACCGAAA 
GTAAAGAAGA AGAAAACTTC AGTGAAACAA GAATGGGATA ATACCGTGAC TGATCTAACC 
GTTCATCGGG CAACTCCCGA AGATCTGGTA CGCCGTCATG AAATACACAA ATCGAAGAAT 
AGAGCATTAG TACACTGGGA ACTCCAAGAA AAAGCTTTGA AGAGAAAATG GAGGAAGCAG 
AAACCAGAAA CTTTAAATCT TGAGAAAAGA AGATTGTCTA TCATGAAGGA GATTCTTTCT 
GATCAATACC AGATGCAAGA TGTGTTGGAG AAATCTGATC ATCTAATAGC TGCAGCAAAA 
GAGCTGTTTC CTCGTAGGCG CACAGGGTTT CCAAATGTAA CAGTGGCTCC TGATTCCTCT 
CAGGGTCCCA TTGTGGTAAA TCAAGACCCT ATCACCCAAT CTATCTTTAA TGAGTCTGTC 
ATAGAACCTC AGGCTCTTAA TGATGTAGAT GGTGAAGAAG AAGGAACTGT TAATAGCCAG 
TCAGGAGAAA GTGAGAATGA GAATGAGTTG GATAACTCTC TAAACTCTCA GTCTAACACG 
AATACAGACA GGTTTCTCCA ACAACTAACA GAAGAGAATT TTGAGTTAAT TAGTAAGTTG 
TGGACTGACA TTCAGCAGAA AATAGCAACC CAGTCACAAA TAACTCCTCC AGGAACGCCA 
TCATCTGCTC TTTCATCAGG GGAGCAAAGA GCTGCTCTGA ATGCTACCAA TGCTGTCAAG 
AGACTCCAAA CCAGGCTTCA GCCTGAAGAA TCTACTGAGA CTCTAGACTC AAGCTACGTT 
GTGGGACACG TGCTGAACTC AAGGAAGCAA AAACAGCTGT TAAATAAAGT GAAAAGGAAA 
CCGAATTTGC ATGCTCTTTC CAAGCCGAAG AAAAACATAT CATCAGGTAG CACAACCTCT 
GCAGACTTAC CAAATAGGAC TAATTCCAAC CTGGATGTCC TCAAACACAT GATACATGAA 
GTGGAACATG AAATGGAAGA ATATGAGCGG TGGACAGGTC GCGAGGTCAA GGGTCTGCAG 
AGCAGTCAGG GTCTTACAGG CTTCACTTTG TCGCTGGTGA GCTCCCTCTG TCGCCTGGTT 
CGGTACCTTA AAGAGAGTGA GATCCAGCTA CGTAAAGAAG TAGAGACAAG GCAACAACTG 
GAACAAGTAT TAGGTGATCA TCGAGAGCTC ATTGATGCTC TGACAGCTGA AATTCTTCGT 
CTTAGAGAAG AAAACGCTGC TACACAGGCA AGACTTCAGC AGTACATGGT CACAACAGAT 
GAGCAACTGA TATCACTCAC ACATGCTATT AAGAACTGTC CTGTGATAAA TAACAGACAA 
GAAATTCAGG CATCAGAAAG CGGAGCCACA GGTAGAAGAG TTATGGACAG TCCAGAGCGT 
CCAGTTGTAA ATGCCAATGT CTCAGTGCCA TTGATGTTCA GAGAGGAAGT GGCTGAATTC 
CCACAGGAAG AGTTGCCCGT TAAACTGTCT CAGGTGCCAG ACCCTCCAGA TAACATGAAT 
CTGGCCAAGA ATTTTCCAGC ACATATTTTT GAGCCAGCTG TGTTGTTAAC ACCACCCAGG 
CAGAAGAGCA ACTTAAAATT CTCTCCTCTT CAGGACGTAT TGAGAAGGAC TGTTCAAACT 
CGTCCTGCTC CACGACTTCC TCCAACTGTG GAAATAATTG AGAAGGAACA AAATTGGGAA 
GAGAAGACCT TACCTATTGA TACAGACATT CAGAATTCAA GTGAAGAGAA TCGTCTCTTC 
ACTCAGAGAT GGAGAGTCTC TCACATGGGA GAAGATTTGG AGAACAAAAC TCAGGCTCCT 
TTTGTTAACC TCTCACAGCC CCTCTGCAAT TCCCATTCCA ACACTCAACA GTCAAGAAGC 
CCCACATTCT CAGAAGAGCT CCCAGTACTG GGAGATGGGC AGCAGCTGAG AACAAATGAG 
TCATTAATAC AAAGAAAGGA CATAATGACA CGAATTGCTG ATTTGACATT GCAGAATTCA 
GCTATCAAGG CACATATGAA TAATATTATT GAGCCCAGAG GAGAGCAAGG GGATGGACTC 
CGGGAGTTGA ACAAACAAGA AAGTGCAAGT GACATGACTT CTACTTTTCC AGTAGCACAG 
TCTCTAACAC CAGGTAGTAT GGAGGAACGG ATTGCAGAAT TGAATCGACA AAGTATGGAG 
GCTCGTGGAA AACTACTGCA GTTGATAGAG CAGCAGAAAC TTGTTGGTTT GAATCTTTCT 
CCACCAATGT CACCTGTTCA GTTACCTCTC AGAGCATGGA CTGAAGGAGC AAAGAGGACA 
ATTGAGGTAT CTATTCCAGG AGCAGAAGCC CCAGAAAGCT CAAAATGTAG TACTGTCTCT 
CCCGTCAGCG GGATAAATAC AAGAAGATCT TCCGGGGCTA CTGGTAATTC TTGTTCTCCA 
CTAAATGCCA CCTCAGGAAG TGGGAGATTC ACACCTCTTA ATCCAAGAGC AAAGATTGAG 
AAACAGAATG AAGAAGGCTG GTTTGCTCTT TCTACCCATG TATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPICE1 ( XM_017005778.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861267.1 Homo sapiens spindle and centriole associated protein 1 (SPICE1), transcript variant X5, mRNA.

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CloneID	OHu13732
Clone ID Related Accession (Same CDS sequence)	XM_017005778.1 , NM_144718.3 , NM_001331078.1 , NM_001331078.2 , NM_144718.4
Accession Version	XM_017005778.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	spindle and centriole-associated protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATTTG TCAGAGTGAA CCGCTGTGGT CCCCGAGTTG GTGTAAGAAA GACACCGAAA 
GTAAAGAAGA AGAAAACTTC AGTGAAACAA GAATGGGATA ATACCGTGAC TGATCTAACC 
GTTCATCGGG CAACTCCCGA AGATCTGGTA CGCCGTCATG AAATACACAA ATCGAAGAAT 
AGAGCATTAG TACACTGGGA ACTCCAAGAA AAAGCTTTGA AGAGAAAATG GAGGAAGCAG 
AAACCAGAAA CTTTAAATCT TGAGAAAAGA AGATTGTCTA TCATGAAGGA GATTCTTTCT 
GATCAATACC AGATGCAAGA TGTGTTGGAG AAATCTGATC ATCTAATAGC TGCAGCAAAA 
GAGCTGTTTC CTCGTAGGCG CACAGGGTTT CCAAATGTAA CAGTGGCTCC TGATTCCTCT 
CAGGGTCCCA TTGTGGTAAA TCAAGACCCT ATCACCCAAT CTATCTTTAA TGAGTCTGTC 
ATAGAACCTC AGGCTCTTAA TGATGTAGAT GGTGAAGAAG AAGGAACTGT TAATAGCCAG 
TCAGGAGAAA GTGAGAATGA GAATGAGTTG GATAACTCTC TAAACTCTCA GTCTAACACG 
AATACAGACA GGTTTCTCCA ACAACTAACA GAAGAGAATT TTGAGTTAAT TAGTAAGTTG 
TGGACTGACA TTCAGCAGAA AATAGCAACC CAGTCACAAA TAACTCCTCC AGGAACGCCA 
TCATCTGCTC TTTCATCAGG GGAGCAAAGA GCTGCTCTGA ATGCTACCAA TGCTGTCAAG 
AGACTCCAAA CCAGGCTTCA GCCTGAAGAA TCTACTGAGA CTCTAGACTC AAGCTACGTT 
GTGGGACACG TGCTGAACTC AAGGAAGCAA AAACAGCTGT TAAATAAAGT GAAAAGGAAA 
CCGAATTTGC ATGCTCTTTC CAAGCCGAAG AAAAACATAT CATCAGGTAG CACAACCTCT 
GCAGACTTAC CAAATAGGAC TAATTCCAAC CTGGATGTCC TCAAACACAT GATACATGAA 
GTGGAACATG AAATGGAAGA ATATGAGCGG TGGACAGGTC GCGAGGTCAA GGGTCTGCAG 
AGCAGTCAGG GTCTTACAGG CTTCACTTTG TCGCTGGTGA GCTCCCTCTG TCGCCTGGTT 
CGGTACCTTA AAGAGAGTGA GATCCAGCTA CGTAAAGAAG TAGAGACAAG GCAACAACTG 
GAACAAGTAT TAGGTGATCA TCGAGAGCTC ATTGATGCTC TGACAGCTGA AATTCTTCGT 
CTTAGAGAAG AAAACGCTGC TACACAGGCA AGACTTCAGC AGTACATGGT CACAACAGAT 
GAGCAACTGA TATCACTCAC ACATGCTATT AAGAACTGTC CTGTGATAAA TAACAGACAA 
GAAATTCAGG CATCAGAAAG CGGAGCCACA GGTAGAAGAG TTATGGACAG TCCAGAGCGT 
CCAGTTGTAA ATGCCAATGT CTCAGTGCCA TTGATGTTCA GAGAGGAAGT GGCTGAATTC 
CCACAGGAAG AGTTGCCCGT TAAACTGTCT CAGGTGCCAG ACCCTCCAGA TAACATGAAT 
CTGGCCAAGA ATTTTCCAGC ACATATTTTT GAGCCAGCTG TGTTGTTAAC ACCACCCAGG 
CAGAAGAGCA ACTTAAAATT CTCTCCTCTT CAGGACGTAT TGAGAAGGAC TGTTCAAACT 
CGTCCTGCTC CACGACTTCC TCCAACTGTG GAAATAATTG AGAAGGAACA AAATTGGGAA 
GAGAAGACCT TACCTATTGA TACAGACATT CAGAATTCAA GTGAAGAGAA TCGTCTCTTC 
ACTCAGAGAT GGAGAGTCTC TCACATGGGA GAAGATTTGG AGAACAAAAC TCAGGCTCCT 
TTTGTTAACC TCTCACAGCC CCTCTGCAAT TCCCATTCCA ACACTCAACA GTCAAGAAGC 
CCCACATTCT CAGAAGAGCT CCCAGTACTG GGAGATGGGC AGCAGCTGAG AACAAATGAG 
TCATTAATAC AAAGAAAGGA CATAATGACA CGAATTGCTG ATTTGACATT GCAGAATTCA 
GCTATCAAGG CACATATGAA TAATATTATT GAGCCCAGAG GAGAGCAAGG GGATGGACTC 
CGGGAGTTGA ACAAACAAGA AAGTGCAAGT GACATGACTT CTACTTTTCC AGTAGCACAG 
TCTCTAACAC CAGGTAGTAT GGAGGAACGG ATTGCAGAAT TGAATCGACA AAGTATGGAG 
GCTCGTGGAA AACTACTGCA GTTGATAGAG CAGCAGAAAC TTGTTGGTTT GAATCTTTCT 
CCACCAATGT CACCTGTTCA GTTACCTCTC AGAGCATGGA CTGAAGGAGC AAAGAGGACA 
ATTGAGGTAT CTATTCCAGG AGCAGAAGCC CCAGAAAGCT CAAAATGTAG TACTGTCTCT 
CCCGTCAGCG GGATAAATAC AAGAAGATCT TCCGGGGCTA CTGGTAATTC TTGTTCTCCA 
CTAAATGCCA CCTCAGGAAG TGGGAGATTC ACACCTCTTA ATCCAAGAGC AAAGATTGAG 
AAACAGAATG AAGAAGGCTG GTTTGCTCTT TCTACCCATG TATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861267.1
CDS	293..2860
Translation	MSFVRVNRCGPRVGVRKTPKVKKKKTSVKQEWDNTVTDLTVHRATPEDLVRRHEIHKSKNRALVHWELQEKALKRKWRKQKPETLNLEKRRLSIMKEILSDQYQMQDVLEKSDHLIAAAKELFPRRRTGFPNVTVAPDSSQGPIVVNQDPITQSIFNESVIEPQALNDVDGEEEGTVNSQSGESENENELDNSLNSQSNTNTDRFLQQLTEENFELISKLWTDIQQKIATQSQITPPGTPSSALSSGEQRAALNATNAVKRLQTRLQPEESTETLDSSYVVGHVLNSRKQKQLLNKVKRKPNLHALSKPKKNISSGSTTSADLPNRTNSNLDVLKHMIHEVEHEMEEYERWTGREVKGLQSSQGLTGFTLSLVSSLCRLVRYLKESEIQLRKEVETRQQLEQVLGDHRELIDALTAEILRLREENAATQARLQQYMVTTDEQLISLTHAIKNCPVINNRQEIQASESGATGRRVMDSPERPVVNANVSVPLMFREEVAEFPQEELPVKLSQVPDPPDNMNLAKNFPAHIFEPAVLLTPPRQKSNLKFSPLQDVLRRTVQTRPAPRLPPTVEIIEKEQNWEEKTLPIDTDIQNSSEENRLFTQRWRVSHMGEDLENKTQAPFVNLSQPLCNSHSNTQQSRSPTFSEELPVLGDGQQLRTNESLIQRKDIMTRIADLTLQNSAIKAHMNNIIEPRGEQGDGLRELNKQESASDMTSTFPVAQSLTPGSMEERIAELNRQSMEARGKLLQLIEQQKLVGLNLSPPMSPVQLPLRAWTEGAKRTIEVSIPGAEAPESSKCSTVSPVSGINTRRSSGATGNSCSPLNATSGSGRFTPLNPRAKIEKQNEEGWFALSTHVS

Target ORF information:

RefSeq Version	XM_017005778.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spindle and centriole associated protein 1 (SPICE1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005778.1

ORF Insert Sequence:

ATGTCATTTG TCAGAGTGAA CCGCTGTGGT CCCCGAGTTG GTGTAAGAAA GACACCGAAA 
GTAAAGAAGA AGAAAACTTC AGTGAAACAA GAATGGGATA ATACCGTGAC TGATCTAACC 
GTTCATCGGG CAACTCCCGA AGATCTGGTA CGCCGTCATG AAATACACAA ATCGAAGAAT 
AGAGCATTAG TACACTGGGA ACTCCAAGAA AAAGCTTTGA AGAGAAAATG GAGGAAGCAG 
AAACCAGAAA CTTTAAATCT TGAGAAAAGA AGATTGTCTA TCATGAAGGA GATTCTTTCT 
GATCAATACC AGATGCAAGA TGTGTTGGAG AAATCTGATC ATCTAATAGC TGCAGCAAAA 
GAGCTGTTTC CTCGTAGGCG CACAGGGTTT CCAAATGTAA CAGTGGCTCC TGATTCCTCT 
CAGGGTCCCA TTGTGGTAAA TCAAGACCCT ATCACCCAAT CTATCTTTAA TGAGTCTGTC 
ATAGAACCTC AGGCTCTTAA TGATGTAGAT GGTGAAGAAG AAGGAACTGT TAATAGCCAG 
TCAGGAGAAA GTGAGAATGA GAATGAGTTG GATAACTCTC TAAACTCTCA GTCTAACACG 
AATACAGACA GGTTTCTCCA ACAACTAACA GAAGAGAATT TTGAGTTAAT TAGTAAGTTG 
TGGACTGACA TTCAGCAGAA AATAGCAACC CAGTCACAAA TAACTCCTCC AGGAACGCCA 
TCATCTGCTC TTTCATCAGG GGAGCAAAGA GCTGCTCTGA ATGCTACCAA TGCTGTCAAG 
AGACTCCAAA CCAGGCTTCA GCCTGAAGAA TCTACTGAGA CTCTAGACTC AAGCTACGTT 
GTGGGACACG TGCTGAACTC AAGGAAGCAA AAACAGCTGT TAAATAAAGT GAAAAGGAAA 
CCGAATTTGC ATGCTCTTTC CAAGCCGAAG AAAAACATAT CATCAGGTAG CACAACCTCT 
GCAGACTTAC CAAATAGGAC TAATTCCAAC CTGGATGTCC TCAAACACAT GATACATGAA 
GTGGAACATG AAATGGAAGA ATATGAGCGG TGGACAGGTC GCGAGGTCAA GGGTCTGCAG 
AGCAGTCAGG GTCTTACAGG CTTCACTTTG TCGCTGGTGA GCTCCCTCTG TCGCCTGGTT 
CGGTACCTTA AAGAGAGTGA GATCCAGCTA CGTAAAGAAG TAGAGACAAG GCAACAACTG 
GAACAAGTAT TAGGTGATCA TCGAGAGCTC ATTGATGCTC TGACAGCTGA AATTCTTCGT 
CTTAGAGAAG AAAACGCTGC TACACAGGCA AGACTTCAGC AGTACATGGT CACAACAGAT 
GAGCAACTGA TATCACTCAC ACATGCTATT AAGAACTGTC CTGTGATAAA TAACAGACAA 
GAAATTCAGG CATCAGAAAG CGGAGCCACA GGTAGAAGAG TTATGGACAG TCCAGAGCGT 
CCAGTTGTAA ATGCCAATGT CTCAGTGCCA TTGATGTTCA GAGAGGAAGT GGCTGAATTC 
CCACAGGAAG AGTTGCCCGT TAAACTGTCT CAGGTGCCAG ACCCTCCAGA TAACATGAAT 
CTGGCCAAGA ATTTTCCAGC ACATATTTTT GAGCCAGCTG TGTTGTTAAC ACCACCCAGG 
CAGAAGAGCA ACTTAAAATT CTCTCCTCTT CAGGACGTAT TGAGAAGGAC TGTTCAAACT 
CGTCCTGCTC CACGACTTCC TCCAACTGTG GAAATAATTG AGAAGGAACA AAATTGGGAA 
GAGAAGACCT TACCTATTGA TACAGACATT CAGAATTCAA GTGAAGAGAA TCGTCTCTTC 
ACTCAGAGAT GGAGAGTCTC TCACATGGGA GAAGATTTGG AGAACAAAAC TCAGGCTCCT 
TTTGTTAACC TCTCACAGCC CCTCTGCAAT TCCCATTCCA ACACTCAACA GTCAAGAAGC 
CCCACATTCT CAGAAGAGCT CCCAGTACTG GGAGATGGGC AGCAGCTGAG AACAAATGAG 
TCATTAATAC AAAGAAAGGA CATAATGACA CGAATTGCTG ATTTGACATT GCAGAATTCA 
GCTATCAAGG CACATATGAA TAATATTATT GAGCCCAGAG GAGAGCAAGG GGATGGACTC 
CGGGAGTTGA ACAAACAAGA AAGTGCAAGT GACATGACTT CTACTTTTCC AGTAGCACAG 
TCTCTAACAC CAGGTAGTAT GGAGGAACGG ATTGCAGAAT TGAATCGACA AAGTATGGAG 
GCTCGTGGAA AACTACTGCA GTTGATAGAG CAGCAGAAAC TTGTTGGTTT GAATCTTTCT 
CCACCAATGT CACCTGTTCA GTTACCTCTC AGAGCATGGA CTGAAGGAGC AAAGAGGACA 
ATTGAGGTAT CTATTCCAGG AGCAGAAGCC CCAGAAAGCT CAAAATGTAG TACTGTCTCT 
CCCGTCAGCG GGATAAATAC AAGAAGATCT TCCGGGGCTA CTGGTAATTC TTGTTCTCCA 
CTAAATGCCA CCTCAGGAAG TGGGAGATTC ACACCTCTTA ATCCAAGAGC AAAGATTGAG 
AAACAGAATG AAGAAGGCTG GTTTGCTCTT TCTACCCATG TATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPICE1 ( NM_001331079.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001318008.1 Homo sapiens spindle and centriole associated protein 1 (SPICE1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu76713
Clone ID Related Accession (Same CDS sequence)	XM_017005775.1 , NM_001331079.1
Accession Version	NM_001331079.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Homo sapiens(human)
Product	spindle and centriole-associated protein 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC112128.4. On Sep 8, 2016 this sequence version replaced XM_011512461.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.26291.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGCGTGC GCACCGCTTT CGGGAGCTTT TGCACAGAGC TGATGTCATT TGTCAGAGTG 
AACCGCTGTG GTCCCCGAGT TGGTGTAAGA AAGACACCGA AAGTAAAGAA GAAGAAAACT 
TCAGTGAAAC AAGAATGGGA TAATACCGTG ACTGATCTAA CCGTTCATCG GGCAACTCCC 
GAAGATCTGG TACGCCGTCA TGAAATACAC AAATCGAAGA ATAGAGCATT AGTACACTGG 
GAACTCCAAG AAAAAGCTTT GAAGAGAAAA TGGAGGAAGC AGAAACCAGA AACTTTAAAT 
CTTGAGAAAA GAAGATTGTC TATCATGAAG GAGATTCTTT CTGATCAATA CCAGATGCAA 
GATGTGTTGG AGAAATCTGA TCATCTAATA GCTGCAGCAA AAGAGCTGTT TCCTCGTAGG 
CGCACAGGGT TTCCAAATGT AACAGTGGCT CCTGATTCCT CTCAGGGTCC CATTGTGGTA 
AATCAAGACC CTATCACCCA ATCTATCTTT AATGAGTCTG TCATAGAACC TCAGGCTCTT 
AATGATGTAG ATGGTGAAGA AGAAGGAACT GTTAATAGCC AGTCAGGAGA AAGTGAGAAT 
GAGAATGAGT TGGATAACTC TCTAAACTCT CAGTCTAACA CGAATACAGA CAGGTTTCTC 
CAACAACTAA CAGAAGAGAA TTTTGAGTTA ATTAGTAAGT TGTGGACTGA CATTCAGCAG 
AAAATAGCAA CCCAGTCACA AATAACTCCT CCAGGAACGC CATCATCTGC TCTTTCATCA 
GGGGAGCAAA GAGCTGCTCT GAATGCTACC AATGCTGTCA AGAGACTCCA AACCAGGCTT 
CAGCCTGAAG AATCTACTGA GACTCTAGAC TCAAGCTACG TTGTGGGACA CGTGCTGAAC 
TCAAGGAAGC AAAAACAGCT GTTAAATAAA GTGAAAAGGA AACCGAATTT GCATGCTCTT 
TCCAAGCCGA AGAAAAACAT ATCATCAGGT AGCACAACCT CTGCAGACTT ACCAAATAGG 
ACTAATTCCA ACCTGGATGT CCTCAAACAC ATGATACATG AAGTGGAACA TGAAATGGAA 
GAATATGAGC GGTGGACAGG TCGCGAGGTC AAGGGTCTGC AGAGCAGTCA GGGTCTTACA 
GGCTTCACTT TGTCGCTGGT GAGCTCCCTC TGTCGCCTGG TTCGGTACCT TAAAGAGAGT 
GAGATCCAGC TACGTAAAGA AGTAGAGACA AGGCAACAAC TGGAACAAGT ATTAGGTGAT 
CATCGAGAGC TCATTGATGC TCTGACAGCT GAAATTCTTC GTCTTAGAGA AGAAAACGCT 
GCTACACAGG CAAGACTTCA GCAGTACATG GTCACAACAG ATGAGCAACT GATATCACTC 
ACACATGCTA TTAAGAACTG TCCTGTGATA AATAACAGAC AAGAAATTCA GGCATCAGAA 
AGCGGAGCCA CAGGTAGAAG AGTTATGGAC AGTCCAGAGC GTCCAGTTGT AAATGCCAAT 
GTCTCAGTGC CATTGATGTT CAGAGAGGAA GTGGCTGAAT TCCCACAGGA AGAGTTGCCC 
GTTAAACTGT CTCAGGTGCC AGACCCTCCA GATAACATGA ATCTGGCCAA GAATTTTCCA 
GCACATATTT TTGAGCCAGC TGTGTTGTTA ACACCACCCA GGCAGAAGAG CAACTTAAAA 
TTCTCTCCTC TTCAGGACGT ATTGAGAAGG ACTGTTCAAA CTCGTCCTGC TCCACGACTT 
CCTCCAACTG TGGAAATAAT TGAGAAGGAA CAAAATTGGG AAGAGAAGAC CTTACCTATT 
GATACAGACA TTCAGAATTC AAGTGAAGAG AATCGTCTCT TCACTCAGAG ATGGAGAGTC 
TCTCACATGG GAGAAGATTT GGAGAACAAA ACTCAGGCTC CTTTTGTTAA CCTCTCACAG 
CCCCTCTGCA ATTCCCATTC CAACACTCAA CAGTCAAGAA GCCCCACATT CTCAGAAGAG 
CTCCCAGTAC TGGGAGATGG GCAGCAGCTG AGAACAAATG AGTCATTAAT ACAAAGAAAG 
GACATAATGA CACGAATTGC TGATTTGACA TTGCAGAATT CAGCTATCAA GGCACATATG 
AATAATATTA TTGAGCCCAG AGGAGAGCAA GGGGATGGAC TCCGGGAGTT GAACAAACAA 
GAAAGTGCAA GTGACATGAC TTCTACTTTT CCAGTAGCAC AGTCTCTAAC ACCAGGTAGT 
ATGGAGGAAC GGATTGCAGA ATTGAATCGA CAAAGTATGG AGGCTCGTGG AAAACTACTG 
CAGTTGATAG AGCAGCAGAA ACTTGTTGGT TTGAATCTTT CTCCACCAAT GTCACCTGTT 
CAGTTACCTC TCAGAGCATG GACTGAAGGA GCAAAGAGGA CAATTGAGGT ATCTATTCCA 
GGAGCAGAAG CCCCAGAAAG CTCAAAATGT AGTACTGTCT CTCCCGTCAG CGGGATAAAT 
ACAAGAAGAT CTTCCGGGGC TACTGGTAAT TCTTGTTCTC CACTAAATGC CACCTCAGGA 
AGTGGGAGAT TCACACCTCT TAATCCAAGA GCAAAGATTG AGAAACAGAA TGAAGAAGGC 
TGGTTTGCTC TTTCTACCCA TGTATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001318008.1
CDS	7..2616
Translation	MRVRTAFGSFCTELMSFVRVNRCGPRVGVRKTPKVKKKKTSVKQEWDNTVTDLTVHRATPEDLVRRHEIHKSKNRALVHWELQEKALKRKWRKQKPETLNLEKRRLSIMKEILSDQYQMQDVLEKSDHLIAAAKELFPRRRTGFPNVTVAPDSSQGPIVVNQDPITQSIFNESVIEPQALNDVDGEEEGTVNSQSGESENENELDNSLNSQSNTNTDRFLQQLTEENFELISKLWTDIQQKIATQSQITPPGTPSSALSSGEQRAALNATNAVKRLQTRLQPEESTETLDSSYVVGHVLNSRKQKQLLNKVKRKPNLHALSKPKKNISSGSTTSADLPNRTNSNLDVLKHMIHEVEHEMEEYERWTGREVKGLQSSQGLTGFTLSLVSSLCRLVRYLKESEIQLRKEVETRQQLEQVLGDHRELIDALTAEILRLREENAATQARLQQYMVTTDEQLISLTHAIKNCPVINNRQEIQASESGATGRRVMDSPERPVVNANVSVPLMFREEVAEFPQEELPVKLSQVPDPPDNMNLAKNFPAHIFEPAVLLTPPRQKSNLKFSPLQDVLRRTVQTRPAPRLPPTVEIIEKEQNWEEKTLPIDTDIQNSSEENRLFTQRWRVSHMGEDLENKTQAPFVNLSQPLCNSHSNTQQSRSPTFSEELPVLGDGQQLRTNESLIQRKDIMTRIADLTLQNSAIKAHMNNIIEPRGEQGDGLRELNKQESASDMTSTFPVAQSLTPGSMEERIAELNRQSMEARGKLLQLIEQQKLVGLNLSPPMSPVQLPLRAWTEGAKRTIEVSIPGAEAPESSKCSTVSPVSGINTRRSSGATGNSCSPLNATSGSGRFTPLNPRAKIEKQNEEGWFALSTHVS

Target ORF information:

RefSeq Version	NM_001331079.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spindle and centriole associated protein 1 (SPICE1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331079.1

ORF Insert Sequence:

ATGCGCGTGC GCACCGCTTT CGGGAGCTTT TGCACAGAGC TGATGTCATT TGTCAGAGTG 
AACCGCTGTG GTCCCCGAGT TGGTGTAAGA AAGACACCGA AAGTAAAGAA GAAGAAAACT 
TCAGTGAAAC AAGAATGGGA TAATACCGTG ACTGATCTAA CCGTTCATCG GGCAACTCCC 
GAAGATCTGG TACGCCGTCA TGAAATACAC AAATCGAAGA ATAGAGCATT AGTACACTGG 
GAACTCCAAG AAAAAGCTTT GAAGAGAAAA TGGAGGAAGC AGAAACCAGA AACTTTAAAT 
CTTGAGAAAA GAAGATTGTC TATCATGAAG GAGATTCTTT CTGATCAATA CCAGATGCAA 
GATGTGTTGG AGAAATCTGA TCATCTAATA GCTGCAGCAA AAGAGCTGTT TCCTCGTAGG 
CGCACAGGGT TTCCAAATGT AACAGTGGCT CCTGATTCCT CTCAGGGTCC CATTGTGGTA 
AATCAAGACC CTATCACCCA ATCTATCTTT AATGAGTCTG TCATAGAACC TCAGGCTCTT 
AATGATGTAG ATGGTGAAGA AGAAGGAACT GTTAATAGCC AGTCAGGAGA AAGTGAGAAT 
GAGAATGAGT TGGATAACTC TCTAAACTCT CAGTCTAACA CGAATACAGA CAGGTTTCTC 
CAACAACTAA CAGAAGAGAA TTTTGAGTTA ATTAGTAAGT TGTGGACTGA CATTCAGCAG 
AAAATAGCAA CCCAGTCACA AATAACTCCT CCAGGAACGC CATCATCTGC TCTTTCATCA 
GGGGAGCAAA GAGCTGCTCT GAATGCTACC AATGCTGTCA AGAGACTCCA AACCAGGCTT 
CAGCCTGAAG AATCTACTGA GACTCTAGAC TCAAGCTACG TTGTGGGACA CGTGCTGAAC 
TCAAGGAAGC AAAAACAGCT GTTAAATAAA GTGAAAAGGA AACCGAATTT GCATGCTCTT 
TCCAAGCCGA AGAAAAACAT ATCATCAGGT AGCACAACCT CTGCAGACTT ACCAAATAGG 
ACTAATTCCA ACCTGGATGT CCTCAAACAC ATGATACATG AAGTGGAACA TGAAATGGAA 
GAATATGAGC GGTGGACAGG TCGCGAGGTC AAGGGTCTGC AGAGCAGTCA GGGTCTTACA 
GGCTTCACTT TGTCGCTGGT GAGCTCCCTC TGTCGCCTGG TTCGGTACCT TAAAGAGAGT 
GAGATCCAGC TACGTAAAGA AGTAGAGACA AGGCAACAAC TGGAACAAGT ATTAGGTGAT 
CATCGAGAGC TCATTGATGC TCTGACAGCT GAAATTCTTC GTCTTAGAGA AGAAAACGCT 
GCTACACAGG CAAGACTTCA GCAGTACATG GTCACAACAG ATGAGCAACT GATATCACTC 
ACACATGCTA TTAAGAACTG TCCTGTGATA AATAACAGAC AAGAAATTCA GGCATCAGAA 
AGCGGAGCCA CAGGTAGAAG AGTTATGGAC AGTCCAGAGC GTCCAGTTGT AAATGCCAAT 
GTCTCAGTGC CATTGATGTT CAGAGAGGAA GTGGCTGAAT TCCCACAGGA AGAGTTGCCC 
GTTAAACTGT CTCAGGTGCC AGACCCTCCA GATAACATGA ATCTGGCCAA GAATTTTCCA 
GCACATATTT TTGAGCCAGC TGTGTTGTTA ACACCACCCA GGCAGAAGAG CAACTTAAAA 
TTCTCTCCTC TTCAGGACGT ATTGAGAAGG ACTGTTCAAA CTCGTCCTGC TCCACGACTT 
CCTCCAACTG TGGAAATAAT TGAGAAGGAA CAAAATTGGG AAGAGAAGAC CTTACCTATT 
GATACAGACA TTCAGAATTC AAGTGAAGAG AATCGTCTCT TCACTCAGAG ATGGAGAGTC 
TCTCACATGG GAGAAGATTT GGAGAACAAA ACTCAGGCTC CTTTTGTTAA CCTCTCACAG 
CCCCTCTGCA ATTCCCATTC CAACACTCAA CAGTCAAGAA GCCCCACATT CTCAGAAGAG 
CTCCCAGTAC TGGGAGATGG GCAGCAGCTG AGAACAAATG AGTCATTAAT ACAAAGAAAG 
GACATAATGA CACGAATTGC TGATTTGACA TTGCAGAATT CAGCTATCAA GGCACATATG 
AATAATATTA TTGAGCCCAG AGGAGAGCAA GGGGATGGAC TCCGGGAGTT GAACAAACAA 
GAAAGTGCAA GTGACATGAC TTCTACTTTT CCAGTAGCAC AGTCTCTAAC ACCAGGTAGT 
ATGGAGGAAC GGATTGCAGA ATTGAATCGA CAAAGTATGG AGGCTCGTGG AAAACTACTG 
CAGTTGATAG AGCAGCAGAA ACTTGTTGGT TTGAATCTTT CTCCACCAAT GTCACCTGTT 
CAGTTACCTC TCAGAGCATG GACTGAAGGA GCAAAGAGGA CAATTGAGGT ATCTATTCCA 
GGAGCAGAAG CCCCAGAAAG CTCAAAATGT AGTACTGTCT CTCCCGTCAG CGGGATAAAT 
ACAAGAAGAT CTTCCGGGGC TACTGGTAAT TCTTGTTCTC CACTAAATGC CACCTCAGGA 
AGTGGGAGAT TCACACCTCT TAATCCAAGA GCAAAGATTG AGAAACAGAA TGAAGAAGGC 
TGGTTTGCTC TTTCTACCCA TGTATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPICE1 ( XM_017005775.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861264.1 Homo sapiens spindle and centriole associated protein 1 (SPICE1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu76713
Clone ID Related Accession (Same CDS sequence)	XM_017005775.1 , NM_001331079.1
Accession Version	XM_017005775.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	spindle and centriole-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGTGC GCACCGCTTT CGGGAGCTTT TGCACAGAGC TGATGTCATT TGTCAGAGTG 
AACCGCTGTG GTCCCCGAGT TGGTGTAAGA AAGACACCGA AAGTAAAGAA GAAGAAAACT 
TCAGTGAAAC AAGAATGGGA TAATACCGTG ACTGATCTAA CCGTTCATCG GGCAACTCCC 
GAAGATCTGG TACGCCGTCA TGAAATACAC AAATCGAAGA ATAGAGCATT AGTACACTGG 
GAACTCCAAG AAAAAGCTTT GAAGAGAAAA TGGAGGAAGC AGAAACCAGA AACTTTAAAT 
CTTGAGAAAA GAAGATTGTC TATCATGAAG GAGATTCTTT CTGATCAATA CCAGATGCAA 
GATGTGTTGG AGAAATCTGA TCATCTAATA GCTGCAGCAA AAGAGCTGTT TCCTCGTAGG 
CGCACAGGGT TTCCAAATGT AACAGTGGCT CCTGATTCCT CTCAGGGTCC CATTGTGGTA 
AATCAAGACC CTATCACCCA ATCTATCTTT AATGAGTCTG TCATAGAACC TCAGGCTCTT 
AATGATGTAG ATGGTGAAGA AGAAGGAACT GTTAATAGCC AGTCAGGAGA AAGTGAGAAT 
GAGAATGAGT TGGATAACTC TCTAAACTCT CAGTCTAACA CGAATACAGA CAGGTTTCTC 
CAACAACTAA CAGAAGAGAA TTTTGAGTTA ATTAGTAAGT TGTGGACTGA CATTCAGCAG 
AAAATAGCAA CCCAGTCACA AATAACTCCT CCAGGAACGC CATCATCTGC TCTTTCATCA 
GGGGAGCAAA GAGCTGCTCT GAATGCTACC AATGCTGTCA AGAGACTCCA AACCAGGCTT 
CAGCCTGAAG AATCTACTGA GACTCTAGAC TCAAGCTACG TTGTGGGACA CGTGCTGAAC 
TCAAGGAAGC AAAAACAGCT GTTAAATAAA GTGAAAAGGA AACCGAATTT GCATGCTCTT 
TCCAAGCCGA AGAAAAACAT ATCATCAGGT AGCACAACCT CTGCAGACTT ACCAAATAGG 
ACTAATTCCA ACCTGGATGT CCTCAAACAC ATGATACATG AAGTGGAACA TGAAATGGAA 
GAATATGAGC GGTGGACAGG TCGCGAGGTC AAGGGTCTGC AGAGCAGTCA GGGTCTTACA 
GGCTTCACTT TGTCGCTGGT GAGCTCCCTC TGTCGCCTGG TTCGGTACCT TAAAGAGAGT 
GAGATCCAGC TACGTAAAGA AGTAGAGACA AGGCAACAAC TGGAACAAGT ATTAGGTGAT 
CATCGAGAGC TCATTGATGC TCTGACAGCT GAAATTCTTC GTCTTAGAGA AGAAAACGCT 
GCTACACAGG CAAGACTTCA GCAGTACATG GTCACAACAG ATGAGCAACT GATATCACTC 
ACACATGCTA TTAAGAACTG TCCTGTGATA AATAACAGAC AAGAAATTCA GGCATCAGAA 
AGCGGAGCCA CAGGTAGAAG AGTTATGGAC AGTCCAGAGC GTCCAGTTGT AAATGCCAAT 
GTCTCAGTGC CATTGATGTT CAGAGAGGAA GTGGCTGAAT TCCCACAGGA AGAGTTGCCC 
GTTAAACTGT CTCAGGTGCC AGACCCTCCA GATAACATGA ATCTGGCCAA GAATTTTCCA 
GCACATATTT TTGAGCCAGC TGTGTTGTTA ACACCACCCA GGCAGAAGAG CAACTTAAAA 
TTCTCTCCTC TTCAGGACGT ATTGAGAAGG ACTGTTCAAA CTCGTCCTGC TCCACGACTT 
CCTCCAACTG TGGAAATAAT TGAGAAGGAA CAAAATTGGG AAGAGAAGAC CTTACCTATT 
GATACAGACA TTCAGAATTC AAGTGAAGAG AATCGTCTCT TCACTCAGAG ATGGAGAGTC 
TCTCACATGG GAGAAGATTT GGAGAACAAA ACTCAGGCTC CTTTTGTTAA CCTCTCACAG 
CCCCTCTGCA ATTCCCATTC CAACACTCAA CAGTCAAGAA GCCCCACATT CTCAGAAGAG 
CTCCCAGTAC TGGGAGATGG GCAGCAGCTG AGAACAAATG AGTCATTAAT ACAAAGAAAG 
GACATAATGA CACGAATTGC TGATTTGACA TTGCAGAATT CAGCTATCAA GGCACATATG 
AATAATATTA TTGAGCCCAG AGGAGAGCAA GGGGATGGAC TCCGGGAGTT GAACAAACAA 
GAAAGTGCAA GTGACATGAC TTCTACTTTT CCAGTAGCAC AGTCTCTAAC ACCAGGTAGT 
ATGGAGGAAC GGATTGCAGA ATTGAATCGA CAAAGTATGG AGGCTCGTGG AAAACTACTG 
CAGTTGATAG AGCAGCAGAA ACTTGTTGGT TTGAATCTTT CTCCACCAAT GTCACCTGTT 
CAGTTACCTC TCAGAGCATG GACTGAAGGA GCAAAGAGGA CAATTGAGGT ATCTATTCCA 
GGAGCAGAAG CCCCAGAAAG CTCAAAATGT AGTACTGTCT CTCCCGTCAG CGGGATAAAT 
ACAAGAAGAT CTTCCGGGGC TACTGGTAAT TCTTGTTCTC CACTAAATGC CACCTCAGGA 
AGTGGGAGAT TCACACCTCT TAATCCAAGA GCAAAGATTG AGAAACAGAA TGAAGAAGGC 
TGGTTTGCTC TTTCTACCCA TGTATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861264.1
CDS	7..2616
Translation	MRVRTAFGSFCTELMSFVRVNRCGPRVGVRKTPKVKKKKTSVKQEWDNTVTDLTVHRATPEDLVRRHEIHKSKNRALVHWELQEKALKRKWRKQKPETLNLEKRRLSIMKEILSDQYQMQDVLEKSDHLIAAAKELFPRRRTGFPNVTVAPDSSQGPIVVNQDPITQSIFNESVIEPQALNDVDGEEEGTVNSQSGESENENELDNSLNSQSNTNTDRFLQQLTEENFELISKLWTDIQQKIATQSQITPPGTPSSALSSGEQRAALNATNAVKRLQTRLQPEESTETLDSSYVVGHVLNSRKQKQLLNKVKRKPNLHALSKPKKNISSGSTTSADLPNRTNSNLDVLKHMIHEVEHEMEEYERWTGREVKGLQSSQGLTGFTLSLVSSLCRLVRYLKESEIQLRKEVETRQQLEQVLGDHRELIDALTAEILRLREENAATQARLQQYMVTTDEQLISLTHAIKNCPVINNRQEIQASESGATGRRVMDSPERPVVNANVSVPLMFREEVAEFPQEELPVKLSQVPDPPDNMNLAKNFPAHIFEPAVLLTPPRQKSNLKFSPLQDVLRRTVQTRPAPRLPPTVEIIEKEQNWEEKTLPIDTDIQNSSEENRLFTQRWRVSHMGEDLENKTQAPFVNLSQPLCNSHSNTQQSRSPTFSEELPVLGDGQQLRTNESLIQRKDIMTRIADLTLQNSAIKAHMNNIIEPRGEQGDGLRELNKQESASDMTSTFPVAQSLTPGSMEERIAELNRQSMEARGKLLQLIEQQKLVGLNLSPPMSPVQLPLRAWTEGAKRTIEVSIPGAEAPESSKCSTVSPVSGINTRRSSGATGNSCSPLNATSGSGRFTPLNPRAKIEKQNEEGWFALSTHVS

Target ORF information:

RefSeq Version	XM_017005775.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spindle and centriole associated protein 1 (SPICE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005775.1

ORF Insert Sequence:

ATGCGCGTGC GCACCGCTTT CGGGAGCTTT TGCACAGAGC TGATGTCATT TGTCAGAGTG 
AACCGCTGTG GTCCCCGAGT TGGTGTAAGA AAGACACCGA AAGTAAAGAA GAAGAAAACT 
TCAGTGAAAC AAGAATGGGA TAATACCGTG ACTGATCTAA CCGTTCATCG GGCAACTCCC 
GAAGATCTGG TACGCCGTCA TGAAATACAC AAATCGAAGA ATAGAGCATT AGTACACTGG 
GAACTCCAAG AAAAAGCTTT GAAGAGAAAA TGGAGGAAGC AGAAACCAGA AACTTTAAAT 
CTTGAGAAAA GAAGATTGTC TATCATGAAG GAGATTCTTT CTGATCAATA CCAGATGCAA 
GATGTGTTGG AGAAATCTGA TCATCTAATA GCTGCAGCAA AAGAGCTGTT TCCTCGTAGG 
CGCACAGGGT TTCCAAATGT AACAGTGGCT CCTGATTCCT CTCAGGGTCC CATTGTGGTA 
AATCAAGACC CTATCACCCA ATCTATCTTT AATGAGTCTG TCATAGAACC TCAGGCTCTT 
AATGATGTAG ATGGTGAAGA AGAAGGAACT GTTAATAGCC AGTCAGGAGA AAGTGAGAAT 
GAGAATGAGT TGGATAACTC TCTAAACTCT CAGTCTAACA CGAATACAGA CAGGTTTCTC 
CAACAACTAA CAGAAGAGAA TTTTGAGTTA ATTAGTAAGT TGTGGACTGA CATTCAGCAG 
AAAATAGCAA CCCAGTCACA AATAACTCCT CCAGGAACGC CATCATCTGC TCTTTCATCA 
GGGGAGCAAA GAGCTGCTCT GAATGCTACC AATGCTGTCA AGAGACTCCA AACCAGGCTT 
CAGCCTGAAG AATCTACTGA GACTCTAGAC TCAAGCTACG TTGTGGGACA CGTGCTGAAC 
TCAAGGAAGC AAAAACAGCT GTTAAATAAA GTGAAAAGGA AACCGAATTT GCATGCTCTT 
TCCAAGCCGA AGAAAAACAT ATCATCAGGT AGCACAACCT CTGCAGACTT ACCAAATAGG 
ACTAATTCCA ACCTGGATGT CCTCAAACAC ATGATACATG AAGTGGAACA TGAAATGGAA 
GAATATGAGC GGTGGACAGG TCGCGAGGTC AAGGGTCTGC AGAGCAGTCA GGGTCTTACA 
GGCTTCACTT TGTCGCTGGT GAGCTCCCTC TGTCGCCTGG TTCGGTACCT TAAAGAGAGT 
GAGATCCAGC TACGTAAAGA AGTAGAGACA AGGCAACAAC TGGAACAAGT ATTAGGTGAT 
CATCGAGAGC TCATTGATGC TCTGACAGCT GAAATTCTTC GTCTTAGAGA AGAAAACGCT 
GCTACACAGG CAAGACTTCA GCAGTACATG GTCACAACAG ATGAGCAACT GATATCACTC 
ACACATGCTA TTAAGAACTG TCCTGTGATA AATAACAGAC AAGAAATTCA GGCATCAGAA 
AGCGGAGCCA CAGGTAGAAG AGTTATGGAC AGTCCAGAGC GTCCAGTTGT AAATGCCAAT 
GTCTCAGTGC CATTGATGTT CAGAGAGGAA GTGGCTGAAT TCCCACAGGA AGAGTTGCCC 
GTTAAACTGT CTCAGGTGCC AGACCCTCCA GATAACATGA ATCTGGCCAA GAATTTTCCA 
GCACATATTT TTGAGCCAGC TGTGTTGTTA ACACCACCCA GGCAGAAGAG CAACTTAAAA 
TTCTCTCCTC TTCAGGACGT ATTGAGAAGG ACTGTTCAAA CTCGTCCTGC TCCACGACTT 
CCTCCAACTG TGGAAATAAT TGAGAAGGAA CAAAATTGGG AAGAGAAGAC CTTACCTATT 
GATACAGACA TTCAGAATTC AAGTGAAGAG AATCGTCTCT TCACTCAGAG ATGGAGAGTC 
TCTCACATGG GAGAAGATTT GGAGAACAAA ACTCAGGCTC CTTTTGTTAA CCTCTCACAG 
CCCCTCTGCA ATTCCCATTC CAACACTCAA CAGTCAAGAA GCCCCACATT CTCAGAAGAG 
CTCCCAGTAC TGGGAGATGG GCAGCAGCTG AGAACAAATG AGTCATTAAT ACAAAGAAAG 
GACATAATGA CACGAATTGC TGATTTGACA TTGCAGAATT CAGCTATCAA GGCACATATG 
AATAATATTA TTGAGCCCAG AGGAGAGCAA GGGGATGGAC TCCGGGAGTT GAACAAACAA 
GAAAGTGCAA GTGACATGAC TTCTACTTTT CCAGTAGCAC AGTCTCTAAC ACCAGGTAGT 
ATGGAGGAAC GGATTGCAGA ATTGAATCGA CAAAGTATGG AGGCTCGTGG AAAACTACTG 
CAGTTGATAG AGCAGCAGAA ACTTGTTGGT TTGAATCTTT CTCCACCAAT GTCACCTGTT 
CAGTTACCTC TCAGAGCATG GACTGAAGGA GCAAAGAGGA CAATTGAGGT ATCTATTCCA 
GGAGCAGAAG CCCCAGAAAG CTCAAAATGT AGTACTGTCT CTCCCGTCAG CGGGATAAAT 
ACAAGAAGAT CTTCCGGGGC TACTGGTAAT TCTTGTTCTC CACTAAATGC CACCTCAGGA 
AGTGGGAGAT TCACACCTCT TAATCCAAGA GCAAAGATTG AGAAACAGAA TGAAGAAGGC 
TGGTTTGCTC TTTCTACCCA TGTATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPICE1 ( XM_017005776.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861265.1 Homo sapiens spindle and centriole associated protein 1 (SPICE1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu93840
Clone ID Related Accession (Same CDS sequence)	XM_017005776.1
Accession Version	XM_017005776.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	spindle and centriole-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTCA GAGGGGCTTC TCAGAAGGCT CCTCCTATGT CATTTGTCAG AGTGAACCGC 
TGTGGTCCCC GAGTTGGTGT AAGAAAGACA CCGAAAGTAA AGAAGAAGAA AACTTCAGTG 
AAACAAGAAT GGGATAATAC CGTGACTGAT CTAACCGTTC ATCGGGCAAC TCCCGAAGAT 
CTGGTACGCC GTCATGAAAT ACACAAATCG AAGAATAGAG CATTAGTACA CTGGGAACTC 
CAAGAAAAAG CTTTGAAGAG AAAATGGAGG AAGCAGAAAC CAGAAACTTT AAATCTTGAG 
AAAAGAAGAT TGTCTATCAT GAAGGAGATT CTTTCTGATC AATACCAGAT GCAAGATGTG 
TTGGAGAAAT CTGATCATCT AATAGCTGCA GCAAAAGAGC TGTTTCCTCG TAGGCGCACA 
GGGTTTCCAA ATGTAACAGT GGCTCCTGAT TCCTCTCAGG GTCCCATTGT GGTAAATCAA 
GACCCTATCA CCCAATCTAT CTTTAATGAG TCTGTCATAG AACCTCAGGC TCTTAATGAT 
GTAGATGGTG AAGAAGAAGG AACTGTTAAT AGCCAGTCAG GAGAAAGTGA GAATGAGAAT 
GAGTTGGATA ACTCTCTAAA CTCTCAGTCT AACACGAATA CAGACAGGTT TCTCCAACAA 
CTAACAGAAG AGAATTTTGA GTTAATTAGT AAGTTGTGGA CTGACATTCA GCAGAAAATA 
GCAACCCAGT CACAAATAAC TCCTCCAGGA ACGCCATCAT CTGCTCTTTC ATCAGGGGAG 
CAAAGAGCTG CTCTGAATGC TACCAATGCT GTCAAGAGAC TCCAAACCAG GCTTCAGCCT 
GAAGAATCTA CTGAGACTCT AGACTCAAGC TACGTTGTGG GACACGTGCT GAACTCAAGG 
AAGCAAAAAC AGCTGTTAAA TAAAGTGAAA AGGAAACCGA ATTTGCATGC TCTTTCCAAG 
CCGAAGAAAA ACATATCATC AGGTAGCACA ACCTCTGCAG ACTTACCAAA TAGGACTAAT 
TCCAACCTGG ATGTCCTCAA ACACATGATA CATGAAGTGG AACATGAAAT GGAAGAATAT 
GAGCGGTGGA CAGGTCGCGA GGTCAAGGGT CTGCAGAGCA GTCAGGGTCT TACAGGCTTC 
ACTTTGTCGC TGGTGAGCTC CCTCTGTCGC CTGGTTCGGT ACCTTAAAGA GAGTGAGATC 
CAGCTACGTA AAGAAGTAGA GACAAGGCAA CAACTGGAAC AAGTATTAGG TGATCATCGA 
GAGCTCATTG ATGCTCTGAC AGCTGAAATT CTTCGTCTTA GAGAAGAAAA CGCTGCTACA 
CAGGCAAGAC TTCAGCAGTA CATGGTCACA ACAGATGAGC AACTGATATC ACTCACACAT 
GCTATTAAGA ACTGTCCTGT GATAAATAAC AGACAAGAAA TTCAGGCATC AGAAAGCGGA 
GCCACAGGTA GAAGAGTTAT GGACAGTCCA GAGCGTCCAG TTGTAAATGC CAATGTCTCA 
GTGCCATTGA TGTTCAGAGA GGAAGTGGCT GAATTCCCAC AGGAAGAGTT GCCCGTTAAA 
CTGTCTCAGG TGCCAGACCC TCCAGATAAC ATGAATCTGG CCAAGAATTT TCCAGCACAT 
ATTTTTGAGC CAGCTGTGTT GTTAACACCA CCCAGGCAGA AGAGCAACTT AAAATTCTCT 
CCTCTTCAGG ACGTATTGAG AAGGACTGTT CAAACTCGTC CTGCTCCACG ACTTCCTCCA 
ACTGTGGAAA TAATTGAGAA GGAACAAAAT TGGGAAGAGA AGACCTTACC TATTGATACA 
GACATTCAGA ATTCAAGTGA AGAGAATCGT CTCTTCACTC AGAGATGGAG AGTCTCTCAC 
ATGGGAGAAG ATTTGGAGAA CAAAACTCAG GCTCCTTTTG TTAACCTCTC ACAGCCCCTC 
TGCAATTCCC ATTCCAACAC TCAACAGTCA AGAAGCCCCA CATTCTCAGA AGAGCTCCCA 
GTACTGGGAG ATGGGCAGCA GCTGAGAACA AATGAGTCAT TAATACAAAG AAAGGACATA 
ATGACACGAA TTGCTGATTT GACATTGCAG AATTCAGCTA TCAAGGCACA TATGAATAAT 
ATTATTGAGC CCAGAGGAGA GCAAGGGGAT GGACTCCGGG AGTTGAACAA ACAAGAAAGT 
GCAAGTGACA TGACTTCTAC TTTTCCAGTA GCACAGTCTC TAACACCAGG TAGTATGGAG 
GAACGGATTG CAGAATTGAA TCGACAAAGT ATGGAGGCTC GTGGAAAACT ACTGCAGTTG 
ATAGAGCAGC AGAAACTTGT TGGTTTGAAT CTTTCTCCAC CAATGTCACC TGTTCAGTTA 
CCTCTCAGAG CATGGACTGA AGGAGCAAAG AGGACAATTG AGGTATCTAT TCCAGGAGCA 
GAAGCCCCAG AAAGCTCAAA ATGTAGTACT GTCTCTCCCG TCAGCGGGAT AAATACAAGA 
AGATCTTCCG GGGCTACTGG TAATTCTTGT TCTCCACTAA ATGCCACCTC AGGAAGTGGG 
AGATTCACAC CTCTTAATCC AAGAGCAAAG ATTGAGAAAC AGAATGAAGA AGGCTGGTTT 
GCTCTTTCTA CCCATGTATC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861265.1
CDS	150..2753
Translation	MDVRGASQKAPPMSFVRVNRCGPRVGVRKTPKVKKKKTSVKQEWDNTVTDLTVHRATPEDLVRRHEIHKSKNRALVHWELQEKALKRKWRKQKPETLNLEKRRLSIMKEILSDQYQMQDVLEKSDHLIAAAKELFPRRRTGFPNVTVAPDSSQGPIVVNQDPITQSIFNESVIEPQALNDVDGEEEGTVNSQSGESENENELDNSLNSQSNTNTDRFLQQLTEENFELISKLWTDIQQKIATQSQITPPGTPSSALSSGEQRAALNATNAVKRLQTRLQPEESTETLDSSYVVGHVLNSRKQKQLLNKVKRKPNLHALSKPKKNISSGSTTSADLPNRTNSNLDVLKHMIHEVEHEMEEYERWTGREVKGLQSSQGLTGFTLSLVSSLCRLVRYLKESEIQLRKEVETRQQLEQVLGDHRELIDALTAEILRLREENAATQARLQQYMVTTDEQLISLTHAIKNCPVINNRQEIQASESGATGRRVMDSPERPVVNANVSVPLMFREEVAEFPQEELPVKLSQVPDPPDNMNLAKNFPAHIFEPAVLLTPPRQKSNLKFSPLQDVLRRTVQTRPAPRLPPTVEIIEKEQNWEEKTLPIDTDIQNSSEENRLFTQRWRVSHMGEDLENKTQAPFVNLSQPLCNSHSNTQQSRSPTFSEELPVLGDGQQLRTNESLIQRKDIMTRIADLTLQNSAIKAHMNNIIEPRGEQGDGLRELNKQESASDMTSTFPVAQSLTPGSMEERIAELNRQSMEARGKLLQLIEQQKLVGLNLSPPMSPVQLPLRAWTEGAKRTIEVSIPGAEAPESSKCSTVSPVSGINTRRSSGATGNSCSPLNATSGSGRFTPLNPRAKIEKQNEEGWFALSTHVS

Target ORF information:

RefSeq Version	XM_017005776.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spindle and centriole associated protein 1 (SPICE1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005776.1

ORF Insert Sequence:

ATGGATGTCA GAGGGGCTTC TCAGAAGGCT CCTCCTATGT CATTTGTCAG AGTGAACCGC 
TGTGGTCCCC GAGTTGGTGT AAGAAAGACA CCGAAAGTAA AGAAGAAGAA AACTTCAGTG 
AAACAAGAAT GGGATAATAC CGTGACTGAT CTAACCGTTC ATCGGGCAAC TCCCGAAGAT 
CTGGTACGCC GTCATGAAAT ACACAAATCG AAGAATAGAG CATTAGTACA CTGGGAACTC 
CAAGAAAAAG CTTTGAAGAG AAAATGGAGG AAGCAGAAAC CAGAAACTTT AAATCTTGAG 
AAAAGAAGAT TGTCTATCAT GAAGGAGATT CTTTCTGATC AATACCAGAT GCAAGATGTG 
TTGGAGAAAT CTGATCATCT AATAGCTGCA GCAAAAGAGC TGTTTCCTCG TAGGCGCACA 
GGGTTTCCAA ATGTAACAGT GGCTCCTGAT TCCTCTCAGG GTCCCATTGT GGTAAATCAA 
GACCCTATCA CCCAATCTAT CTTTAATGAG TCTGTCATAG AACCTCAGGC TCTTAATGAT 
GTAGATGGTG AAGAAGAAGG AACTGTTAAT AGCCAGTCAG GAGAAAGTGA GAATGAGAAT 
GAGTTGGATA ACTCTCTAAA CTCTCAGTCT AACACGAATA CAGACAGGTT TCTCCAACAA 
CTAACAGAAG AGAATTTTGA GTTAATTAGT AAGTTGTGGA CTGACATTCA GCAGAAAATA 
GCAACCCAGT CACAAATAAC TCCTCCAGGA ACGCCATCAT CTGCTCTTTC ATCAGGGGAG 
CAAAGAGCTG CTCTGAATGC TACCAATGCT GTCAAGAGAC TCCAAACCAG GCTTCAGCCT 
GAAGAATCTA CTGAGACTCT AGACTCAAGC TACGTTGTGG GACACGTGCT GAACTCAAGG 
AAGCAAAAAC AGCTGTTAAA TAAAGTGAAA AGGAAACCGA ATTTGCATGC TCTTTCCAAG 
CCGAAGAAAA ACATATCATC AGGTAGCACA ACCTCTGCAG ACTTACCAAA TAGGACTAAT 
TCCAACCTGG ATGTCCTCAA ACACATGATA CATGAAGTGG AACATGAAAT GGAAGAATAT 
GAGCGGTGGA CAGGTCGCGA GGTCAAGGGT CTGCAGAGCA GTCAGGGTCT TACAGGCTTC 
ACTTTGTCGC TGGTGAGCTC CCTCTGTCGC CTGGTTCGGT ACCTTAAAGA GAGTGAGATC 
CAGCTACGTA AAGAAGTAGA GACAAGGCAA CAACTGGAAC AAGTATTAGG TGATCATCGA 
GAGCTCATTG ATGCTCTGAC AGCTGAAATT CTTCGTCTTA GAGAAGAAAA CGCTGCTACA 
CAGGCAAGAC TTCAGCAGTA CATGGTCACA ACAGATGAGC AACTGATATC ACTCACACAT 
GCTATTAAGA ACTGTCCTGT GATAAATAAC AGACAAGAAA TTCAGGCATC AGAAAGCGGA 
GCCACAGGTA GAAGAGTTAT GGACAGTCCA GAGCGTCCAG TTGTAAATGC CAATGTCTCA 
GTGCCATTGA TGTTCAGAGA GGAAGTGGCT GAATTCCCAC AGGAAGAGTT GCCCGTTAAA 
CTGTCTCAGG TGCCAGACCC TCCAGATAAC ATGAATCTGG CCAAGAATTT TCCAGCACAT 
ATTTTTGAGC CAGCTGTGTT GTTAACACCA CCCAGGCAGA AGAGCAACTT AAAATTCTCT 
CCTCTTCAGG ACGTATTGAG AAGGACTGTT CAAACTCGTC CTGCTCCACG ACTTCCTCCA 
ACTGTGGAAA TAATTGAGAA GGAACAAAAT TGGGAAGAGA AGACCTTACC TATTGATACA 
GACATTCAGA ATTCAAGTGA AGAGAATCGT CTCTTCACTC AGAGATGGAG AGTCTCTCAC 
ATGGGAGAAG ATTTGGAGAA CAAAACTCAG GCTCCTTTTG TTAACCTCTC ACAGCCCCTC 
TGCAATTCCC ATTCCAACAC TCAACAGTCA AGAAGCCCCA CATTCTCAGA AGAGCTCCCA 
GTACTGGGAG ATGGGCAGCA GCTGAGAACA AATGAGTCAT TAATACAAAG AAAGGACATA 
ATGACACGAA TTGCTGATTT GACATTGCAG AATTCAGCTA TCAAGGCACA TATGAATAAT 
ATTATTGAGC CCAGAGGAGA GCAAGGGGAT GGACTCCGGG AGTTGAACAA ACAAGAAAGT 
GCAAGTGACA TGACTTCTAC TTTTCCAGTA GCACAGTCTC TAACACCAGG TAGTATGGAG 
GAACGGATTG CAGAATTGAA TCGACAAAGT ATGGAGGCTC GTGGAAAACT ACTGCAGTTG 
ATAGAGCAGC AGAAACTTGT TGGTTTGAAT CTTTCTCCAC CAATGTCACC TGTTCAGTTA 
CCTCTCAGAG CATGGACTGA AGGAGCAAAG AGGACAATTG AGGTATCTAT TCCAGGAGCA 
GAAGCCCCAG AAAGCTCAAA ATGTAGTACT GTCTCTCCCG TCAGCGGGAT AAATACAAGA 
AGATCTTCCG GGGCTACTGG TAATTCTTGT TCTCCACTAA ATGCCACCTC AGGAAGTGGG 
AGATTCACAC CTCTTAATCC AAGAGCAAAG ATTGAGAAAC AGAATGAAGA AGGCTGGTTT 
GCTCTTTCTA CCCATGTATC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface.
Cell163(6)1484-99(2015 Dec)
Gupta GD,Coyaud ?,Gon?alves J,Mojarad BA,Liu Y,Wu Q,Gheiratmand L,Comartin D,Tkach JM,Cheung SW,Bashkurov M,Hasegan M,Knight JD,Lin ZY,Schueler M,Hildebrandt F,Moffat J,Gingras AC,Raught B,Pelletier L

CEP120 and SPICE1 cooperate with CPAP in centriole elongation.
Current biology : CB23(14)1360-6(2013 Jul)
Comartin D,Gupta GD,Fussner E,Coyaud ?,Hasegan M,Archinti M,Cheung SW,Pinchev D,Lawo S,Raught B,Bazett-Jones DP,L?ders J,Pelletier L

A Y2H-seq approach defines the human protein methyltransferase interactome.
Nature methods10(4)339-42(2013 Apr)
Weimann M,Grossmann A,Woodsmith J,?zkan Z,Birth P,Meierhofer D,Benlasfer N,Valovka T,Timmermann B,Wanker EE,Sauer S,Stelzl U

Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods.
The EMBO journal30(8)1520-35(2011 Apr)
Jakobsen L,Vanselow K,Skogs M,Toyoda Y,Lundberg E,Poser I,Falkenby LG,Bennetzen M,Westendorf J,Nigg EA,Uhlen M,Hyman AA,Andersen JS

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SPICE1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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