CST6 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CST6
Entrez Gene ID	1474
Full Name	cystatin E/M
General protein information	Preferred Names cystatin E/M Names cystatin-M cystatin 6 cystatin M/E cystatin-E cysteine proteinase inhibitor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q13.1
Summary	The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. This gene encodes a cystatin from the type 2 family, which is down-regulated in metastatic breast tumor cells as compared to primary tumor cells. Loss of expression is likely associated with the progression of a primary tumor to a metastatic phenotype. [provided by RefSeq, Jul 2008].
Disorder MIM:	601891

mRNA and Protein(s)

mRNA	Protein	Name
NM_001323.3	NP_001314.1	cystatin-M precursor
NM_001323.4	NP_001314.1	cystatin-M precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	CST6	NP_001314.1
Pan troglodytes (chimpanzee)	CST6	XP_522067.1
	CST6	XP_001111664.2
Rattus norvegicus (Norway rat)	Cst6	NP_598250.1
Bos taurus (cattle)	CST6	NP_001012782.1
Mus musculus (house mouse)	Cst6	NP_082899.1
Canis lupus familiaris (dog)	CST6	XP_540840.2

Gene Ontology
Bibliography

Related articles in PubMed

Low-level internalization of cystatin E/M affects legumain activity and migration of melanoma cells.
Wallin H, Apelqvist J, Andersson F, Ekstr?m U, Abrahamson M
The Journal of biological chemistry292(35)14413-14424(2017 09)

Cystatin E/M Suppresses Tumor Cell Growth through Cytoplasmic Retention of NF-?B.
Soh H, Venkatesan N, Veena MS, Ravichandran S, Zinabadi A, Basak SK, Parvatiyar K, Srivastava M, Liang LJ, Gjertson DW, Torres JZ, Moatamed NA, Srivatsan ES
Molecular and cellular biology36(12)1776-92(2016 06)

Genome-wide association study identifies three novel susceptibility loci for severe Acne vulgaris.
Navarini AA, Simpson MA, Weale M, Knight J, Carlavan I, Reiniche P, Burden DA, Layton A, Bataille V, Allen M, Pleass R, Pink A, Creamer D, English J, Munn S, Walton S, , Willis C, D?ret S, Voegel JJ, Spector T, Smith CH, Trembath RC, Barker JN
Nature communications54020(2014 Jun)

TBX2 represses CST6 resulting in uncontrolled legumain activity to sustain breast cancer proliferation: a novel cancer-selective target pathway with therapeutic opportunities.
D'Costa ZC, Higgins C, Ong CW, Irwin GW, Boyle D, McArt DG, McCloskey K, Buckley NE, Crawford NT, Thiagarajan L, Murray JT, Kennedy RD, Mulligan KA, Harkin DP, Waugh DJ, Scott CJ, Salto-Tellez M, Williams R, Mullan PB
Oncotarget5(6)1609-20(2014 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that melanoma cells internalize/absorb cystatin C from culture media, leading to increased intracellular cystatin C levels; cystatin E/M is internalized as well but at modest rate due to down-regulation of cell migration; however, the effect of intracellular cystatin E/M on down-regulation of legumain activity is pronounced.
Title: Low-level internalization of cystatin E/M affects legumain activity and migration of melanoma cells.

Results provide evidence that cystatin E/M is a tumor suppressor gene which plays an important role in the regulation of NF-kappaB.
Title: Cystatin E/M Suppresses Tumor Cell Growth through Cytoplasmic Retention of NF-?B.

Low CST6 expression is associated with breast cancer.
Title: TBX2 represses CST6 resulting in uncontrolled legumain activity to sustain breast cancer proliferation: a novel cancer-selective target pathway with therapeutic opportunities.

The CST6 promoter is highly methylated in circulating cell-free DNA of breast cancer patients, but not in healthy individuals.
Title: CST6 promoter methylation in circulating cell-free DNA of breast cancer patients.

Developed a Methylation-Sensitive High Resolution Melting Analysis (MS-HRMA) assay for the investigation of CST6 promoter methylation in breast cancer patients.CST6 promoter was methylated in 39/80 of FFPEs.
Title: A closed-tube methylation-sensitive high resolution melting assay (MS-HRMA) for the semi-quantitative determination of CST6 promoter methylation in clinical samples.

The following CST6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CST6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02870	NM_001323.4 Latest version!	Homo sapiens cystatin E/M (CST6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu02870	NM_001323.3	Homo sapiens cystatin E/M (CST6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CST6 ( NM_001323.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001314.1 Homo sapiens cystatin E/M (CST6), mRNA.

Price & Availability↑

CloneID	OHu02870
Clone ID Related Accession (Same CDS sequence)	NM_001323.3 , NM_001323.4
Accession Version	NM_001323.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	cystatin-M precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI127639.1, U81233.1 and AA778147.1. On Mar 4, 2011 this sequence version replaced NM_001323.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279842.6047.1, ERR279831.3218.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCGTT CGAACCTCCC GCTGGCGCTG GGCCTGGCCC TGGTCGCATT CTGCCTCCTG 
GCGCTGCCAC GCGACGCCCG GGCCCGGCCG CAGGAGCGCA TGGTCGGAGA ACTCCGGGAC 
CTGTCGCCCG ACGACCCGCA GGTGCAGAAG GCGGCGCAGG CGGCCGTGGC CAGCTACAAC 
ATGGGCAGCA ACAGCATCTA CTACTTCCGA GACACGCACA TCATCAAGGC GCAGAGCCAG 
CTGGTGGCCG GCATCAAGTA CTTCCTGACG ATGGAGATGG GGAGCACAGA CTGCCGCAAG 
ACCAGGGTCA CTGGAGACCA CGTCGACCTC ACCACTTGCC CCCTGGCAGC AGGGGCGCAG 
CAGGAGAAGC TGCGCTGTGA CTTTGAGGTC CTTGTGGTTC CCTGGCAGAA CTCCTCTCAG 
CTCCTAAAGC ACAACTGTGT GCAGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001314.1
CDS	55..504
Translation	MARSNLPLALGLALVAFCLLALPRDARARPQERMVGELRDLSPDDPQVQKAAQAAVASYNMGSNSIYYFRDTHIIKAQSQLVAGIKYFLTMEMGSTDCRKTRVTGDHVDLTTCPLAAGAQQEKLRCDFEVLVVPWQNSSQLLKHNCVQM

Target ORF information:

RefSeq Version	NM_001323.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cystatin E/M (CST6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001323.3

ORF Insert Sequence:

ATGGCGCGTT CGAACCTCCC GCTGGCGCTG GGCCTGGCCC TGGTCGCATT CTGCCTCCTG 
GCGCTGCCAC GCGACGCCCG GGCCCGGCCG CAGGAGCGCA TGGTCGGAGA ACTCCGGGAC 
CTGTCGCCCG ACGACCCGCA GGTGCAGAAG GCGGCGCAGG CGGCCGTGGC CAGCTACAAC 
ATGGGCAGCA ACAGCATCTA CTACTTCCGA GACACGCACA TCATCAAGGC GCAGAGCCAG 
CTGGTGGCCG GCATCAAGTA CTTCCTGACG ATGGAGATGG GGAGCACAGA CTGCCGCAAG 
ACCAGGGTCA CTGGAGACCA CGTCGACCTC ACCACTTGCC CCCTGGCAGC AGGGGCGCAG 
CAGGAGAAGC TGCGCTGTGA CTTTGAGGTC CTTGTGGTTC CCTGGCAGAA CTCCTCTCAG 
CTCCTAAAGC ACAACTGTGT GCAGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CST6 ( NM_001323.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001314.1 Homo sapiens cystatin E/M (CST6), mRNA.

Price & Availability↑

CloneID	OHu02870
Clone ID Related Accession (Same CDS sequence)	NM_001323.3 , NM_001323.4
Accession Version	NM_001323.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-15
Organism	Homo sapiens(human)
Product	cystatin-M precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI127639.1, U81233.1 and AA778147.1. On Nov 22, 2018 this sequence version replaced NM_001323.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U81233.1, BQ685679.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000312134.3/ ENSP00000311313.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGCGTT CGAACCTCCC GCTGGCGCTG GGCCTGGCCC TGGTCGCATT CTGCCTCCTG 
GCGCTGCCAC GCGACGCCCG GGCCCGGCCG CAGGAGCGCA TGGTCGGAGA ACTCCGGGAC 
CTGTCGCCCG ACGACCCGCA GGTGCAGAAG GCGGCGCAGG CGGCCGTGGC CAGCTACAAC 
ATGGGCAGCA ACAGCATCTA CTACTTCCGA GACACGCACA TCATCAAGGC GCAGAGCCAG 
CTGGTGGCCG GCATCAAGTA CTTCCTGACG ATGGAGATGG GGAGCACAGA CTGCCGCAAG 
ACCAGGGTCA CTGGAGACCA CGTCGACCTC ACCACTTGCC CCCTGGCAGC AGGGGCGCAG 
CAGGAGAAGC TGCGCTGTGA CTTTGAGGTC CTTGTGGTTC CCTGGCAGAA CTCCTCTCAG 
CTCCTAAAGC ACAACTGTGT GCAGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001314.1
CDS	38..487
Translation	MARSNLPLALGLALVAFCLLALPRDARARPQERMVGELRDLSPDDPQVQKAAQAAVASYNMGSNSIYYFRDTHIIKAQSQLVAGIKYFLTMEMGSTDCRKTRVTGDHVDLTTCPLAAGAQQEKLRCDFEVLVVPWQNSSQLLKHNCVQM

Target ORF information:

RefSeq Version	NM_001323.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cystatin E/M (CST6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001323.4

ORF Insert Sequence:

ATGGCGCGTT CGAACCTCCC GCTGGCGCTG GGCCTGGCCC TGGTCGCATT CTGCCTCCTG 
GCGCTGCCAC GCGACGCCCG GGCCCGGCCG CAGGAGCGCA TGGTCGGAGA ACTCCGGGAC 
CTGTCGCCCG ACGACCCGCA GGTGCAGAAG GCGGCGCAGG CGGCCGTGGC CAGCTACAAC 
ATGGGCAGCA ACAGCATCTA CTACTTCCGA GACACGCACA TCATCAAGGC GCAGAGCCAG 
CTGGTGGCCG GCATCAAGTA CTTCCTGACG ATGGAGATGG GGAGCACAGA CTGCCGCAAG 
ACCAGGGTCA CTGGAGACCA CGTCGACCTC ACCACTTGCC CCCTGGCAGC AGGGGCGCAG 
CAGGAGAAGC TGCGCTGTGA CTTTGAGGTC CTTGTGGTTC CCTGGCAGAA CTCCTCTCAG 
CTCCTAAAGC ACAACTGTGT GCAGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Low-level internalization of cystatin E/M affects legumain activity and migration of melanoma cells.
The Journal of biological chemistry292(35)14413-14424(2017 09)
Wallin H,Apelqvist J,Andersson F,Ekstr?m U,Abrahamson M

Cystatin E/M Suppresses Tumor Cell Growth through Cytoplasmic Retention of NF-?B.
Molecular and cellular biology36(12)1776-92(2016 06)
Soh H,Venkatesan N,Veena MS,Ravichandran S,Zinabadi A,Basak SK,Parvatiyar K,Srivastava M,Liang LJ,Gjertson DW,Torres JZ,Moatamed NA,Srivatsan ES

Genome-wide association study identifies three novel susceptibility loci for severe Acne vulgaris.
Nature communications54020(2014 Jun)
Navarini AA,Simpson MA,Weale M,Knight J,Carlavan I,Reiniche P,Burden DA,Layton A,Bataille V,Allen M,Pleass R,Pink A,Creamer D,English J,Munn S,Walton S,,Willis C,D?ret S,Voegel JJ,Spector T,Smith CH,Trembath RC,Barker JN

TBX2 represses CST6 resulting in uncontrolled legumain activity to sustain breast cancer proliferation: a novel cancer-selective target pathway with therapeutic opportunities.
Oncotarget5(6)1609-20(2014 Mar)
D'Costa ZC,Higgins C,Ong CW,Irwin GW,Boyle D,McArt DG,McCloskey K,Buckley NE,Crawford NT,Thiagarajan L,Murray JT,Kennedy RD,Mulligan KA,Harkin DP,Waugh DJ,Scott CJ,Salto-Tellez M,Williams R,Mullan PB

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CST6 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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