CHCHD4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHCHD4
Entrez Gene ID	131474
Full Name	coiled-coil-helix-coiled-coil-helix domain containing 4
Synonyms	MIA40,TIMM40
General protein information	Preferred Names coiled-coil-helix-coiled-coil-helix domain containing 4 Names mitochondrial intermembrane space import and assembly protein 40 coiled-coil-helix-coiled-coil-helix domain-containing protein 4 translocase of inner mitochondrial membrane 40 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p25.1
Summary	CHCHD4, a component of human mitochondria, belongs to a protein family whose members share 6 highly conserved cysteine residues constituting a -CXC-CX(9)C-CX(9)C- motif in the C terminus (Hofmann et al., 2005 [PubMed 16185709]).[supplied by OMIM, Mar 2008].
Disorder MIM:	611077

mRNA and Protein(s)

mRNA	Protein	Name
NM_001098502.1	NP_001091972.1	mitochondrial intermembrane space import and assembly protein 40 isoform 1
NM_001098502.2	NP_001091972.1	mitochondrial intermembrane space import and assembly protein 40 isoform 1
NM_144636.2	NP_653237.1	mitochondrial intermembrane space import and assembly protein 40 isoform 2
NM_144636.3	NP_653237.1	mitochondrial intermembrane space import and assembly protein 40 isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Chchd4	NP_598689.1
Gallus gallus (chicken)	CHCHD4	NP_001107231.1
Homo sapiens (human)	CHCHD4	NP_001091972.1
Danio rerio (zebrafish)	chchd4	NP_001003538.1
Bos taurus (cattle)	CHCHD4	NP_001039624.1
Rattus norvegicus (Norway rat)	Chchd4	NP_001013449.1
Rattus norvegicus (Norway rat)	LOC100361898	XP_003749131.1
Drosophila melanogaster (fruit fly)	CG7950	NP_651770.1
Canis lupus familiaris (dog)	CHCHD4	XP_533726.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002940	XP_311964.4
Xenopus tropicalis (tropical clawed frog)	chchd4	NP_988878.1
Pan troglodytes (chimpanzee)	CHCHD4	XP_001157417.1
	CHCHD4	XP_001091484.1

Gene Ontology
Bibliography

Related articles in PubMed

Direct structural evidence of protein redox regulation obtained by in-cell NMR.
Mercatelli E, Barbieri L, Luchinat E, Banci L
Biochimica et biophysica acta1863(2)198-204(2016 Feb)

Human mitochondrial MIA40 (CHCHD4) is a component of the Fe-S cluster export machinery.
Murari A, Thiriveedi VR, Mohammad F, Vengaldas V, Gorla M, Tammineni P, Krishnamoorthy T, Sepuri NB
The Biochemical journal471(2)231-41(2015 Oct)

The Ca(2+)-Dependent Release of the Mia40-Induced MICU1-MICU2 Dimer from MCU Regulates Mitochondrial Ca(2+) Uptake.
Petrungaro C, Zimmermann KM, K?ttner V, Fischer M, Dengjel J, Bogeski I, Riemer J
Cell metabolism22(4)721-33(2015 Oct)

Metabolic epistasis among apoptosis-inducing factor and the mitochondrial import factor CHCHD4.
Modjtahedi N, Hangen E, Gonin P, Kroemer G
Cell cycle (Georgetown, Tex.)14(17)2743-7(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that the redox state of cytochrome c oxidase assembly protein 17 (Cox17), mitochondrial membrane transport protein Mia40 and superoxide dismutase 1 (SOD1) in the cytoplasm were directly observed with in-cell NMR spectroscopy.
Title: Direct structural evidence of protein redox regulation obtained by in-cell NMR.

results demonstrate an indispensable role for hMIA40 for the export of Fe-S clusters from mitochondria.
Title: Human mitochondrial MIA40 (CHCHD4) is a component of the Fe-S cluster export machinery.

In aggregate these data suggest that the Mia40/lfALR system has a broad sequence specificity and that potential substrates may be protected from adventitious oxidation by kinetic sequestration within the mitochondrial IMS.
Title: Mia40 is a facile oxidant of unfolded reduced proteins but shows minimal isomerase activity.

our findings suggest that MIA40 reduction contributes to the effects of AIF deficiency on OXPHOS, as it may impact on the correct assembly and maintenance of the respiratory subunits.
Title: Loss of apoptosis-inducing factor critically affects MIA40 function.

Compared to wild type control littermates, mice with a knockout of CHCHD4 exhibit reduced weight gain when fed a high-fat diet.
Title: Metabolic epistasis among apoptosis-inducing factor and the mitochondrial import factor CHCHD4.

The following CHCHD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHCHD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17302	NM_001098502.2 Latest version!	Homo sapiens coiled-coil-helix-coiled-coil-helix domain containing 4 (CHCHD4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu17276	NM_144636.3 Latest version!	Homo sapiens coiled-coil-helix-coiled-coil-helix domain containing 4 (CHCHD4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu17302	NM_001098502.1	Homo sapiens coiled-coil-helix-coiled-coil-helix domain containing 4 (CHCHD4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu17276	NM_144636.2	Homo sapiens coiled-coil-helix-coiled-coil-helix domain containing 4 (CHCHD4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHCHD4 ( NM_001098502.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001091972.1 Homo sapiens coiled-coil-helix-coiled-coil-helix domain containing 4 (CHCHD4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17302
Clone ID Related Accession (Same CDS sequence)	NM_001098502.1 , NM_001098502.2
Accession Version	NM_001098502.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-29
Organism	Homo sapiens(human)
Product	mitochondrial intermembrane space import and assembly protein 40 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB452249.1, DB501565.1, BC033775.1 and BC017082.1. Transcript Variant: This variant (1) is the predominant transcript. It does not contain an N-terminal, cleavable mitochondrial target sequence, yet experimental studies have determined that the encoded protein (isoform 1) is found within the intermembrane space of the mitochondrion. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.563132.1, SRR1163655.93879.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGTCCTATT GCCGGCAGGA AGGGAAGGAT CGAATCATAT TTGTAACCAA AGAAGATCAT 
GAAACTCCAA GCAGTGCAGA ATTGGTGGCT GATGACCCCA ACGATCCATA CGAGGAGCAT 
GGATTGATAC TGCCAAATGG AAACATTAAC TGGAACTGCC CATGCCTTGG GGGAATGGCC 
AGCGGTCCCT GTGGAGAACA GTTTAAGTCA GCCTTTTCCT GCTTCCACTA TAGCACGGAG 
GAGATCAAGG GGTCAGACTG TGTAGACCAG TTCCGGGCCA TGCAGGAATG CATGCAGAAA 
TACCCAGACC TCTATCCCCA AGAGGATGAG GATGAGGAAG AGGAAAGAGA GAAGAAGCCA 
GCAGAACAAG CAGAAGAAAC AGCTCCCATT GAGGCCACTG CAACCAAAGA AGAGGAGGGA 
TCAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091972.1
CDS	196..624
Translation	MSYCRQEGKDRIIFVTKEDHETPSSAELVADDPNDPYEEHGLILPNGNINWNCPCLGGMASGPCGEQFKSAFSCFHYSTEEIKGSDCVDQFRAMQECMQKYPDLYPQEDEDEEEEREKKPAEQAEETAPIEATATKEEEGSS

Target ORF information:

RefSeq Version	NM_001098502.1
Organism	Homo sapiens(human)
Definition	Homo sapiens coiled-coil-helix-coiled-coil-helix domain containing 4 (CHCHD4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098502.1

ORF Insert Sequence:

ATGTCCTATT GCCGGCAGGA AGGGAAGGAT CGAATCATAT TTGTAACCAA AGAAGATCAT 
GAAACTCCAA GCAGTGCAGA ATTGGTGGCT GATGACCCCA ACGATCCATA CGAGGAGCAT 
GGATTGATAC TGCCAAATGG AAACATTAAC TGGAACTGCC CATGCCTTGG GGGAATGGCC 
AGCGGTCCCT GTGGAGAACA GTTTAAGTCA GCCTTTTCCT GCTTCCACTA TAGCACGGAG 
GAGATCAAGG GGTCAGACTG TGTAGACCAG TTCCGGGCCA TGCAGGAATG CATGCAGAAA 
TACCCAGACC TCTATCCCCA AGAGGATGAG GATGAGGAAG AGGAAAGAGA GAAGAAGCCA 
GCAGAACAAG CAGAAGAAAC AGCTCCCATT GAGGCCACTG CAACCAAAGA AGAGGAGGGA 
TCAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHCHD4 ( NM_001098502.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001091972.1 Homo sapiens coiled-coil-helix-coiled-coil-helix domain containing 4 (CHCHD4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17302
Clone ID Related Accession (Same CDS sequence)	NM_001098502.1 , NM_001098502.2
Accession Version	NM_001098502.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	mitochondrial intermembrane space import and assembly protein 40 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB452249.1, DB501565.1, BC033775.1 and BC017082.1. On Nov 23, 2018 this sequence version replaced NM_001098502.1. Transcript Variant: This variant (1) is the predominant transcript. It does not contain an N-terminal, cleavable mitochondrial target sequence, yet experimental studies have determined that the encoded protein (isoform 1) is found within the intermembrane space of the mitochondrion. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.184713.1, SRR3476690.125539.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000396914.4/ ENSP00000380122.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCCTATT GCCGGCAGGA AGGGAAGGAT CGAATCATAT TTGTAACCAA AGAAGATCAT 
GAAACTCCAA GCAGTGCAGA ATTGGTGGCT GATGACCCCA ACGATCCATA CGAGGAGCAT 
GGATTGATAC TGCCAAATGG AAACATTAAC TGGAACTGCC CATGCCTTGG GGGAATGGCC 
AGCGGTCCCT GTGGAGAACA GTTTAAGTCA GCCTTTTCCT GCTTCCACTA TAGCACGGAG 
GAGATCAAGG GGTCAGACTG TGTAGACCAG TTCCGGGCCA TGCAGGAATG CATGCAGAAA 
TACCCAGACC TCTATCCCCA AGAGGATGAG GATGAGGAAG AGGAAAGAGA GAAGAAGCCA 
GCAGAACAAG CAGAAGAAAC AGCTCCCATT GAGGCCACTG CAACCAAAGA AGAGGAGGGA 
TCAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091972.1
CDS	195..623
Translation	MSYCRQEGKDRIIFVTKEDHETPSSAELVADDPNDPYEEHGLILPNGNINWNCPCLGGMASGPCGEQFKSAFSCFHYSTEEIKGSDCVDQFRAMQECMQKYPDLYPQEDEDEEEEREKKPAEQAEETAPIEATATKEEEGSS

Target ORF information:

RefSeq Version	NM_001098502.2
Organism	Homo sapiens(human)
Definition	Homo sapiens coiled-coil-helix-coiled-coil-helix domain containing 4 (CHCHD4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098502.2

ORF Insert Sequence:

ATGTCCTATT GCCGGCAGGA AGGGAAGGAT CGAATCATAT TTGTAACCAA AGAAGATCAT 
GAAACTCCAA GCAGTGCAGA ATTGGTGGCT GATGACCCCA ACGATCCATA CGAGGAGCAT 
GGATTGATAC TGCCAAATGG AAACATTAAC TGGAACTGCC CATGCCTTGG GGGAATGGCC 
AGCGGTCCCT GTGGAGAACA GTTTAAGTCA GCCTTTTCCT GCTTCCACTA TAGCACGGAG 
GAGATCAAGG GGTCAGACTG TGTAGACCAG TTCCGGGCCA TGCAGGAATG CATGCAGAAA 
TACCCAGACC TCTATCCCCA AGAGGATGAG GATGAGGAAG AGGAAAGAGA GAAGAAGCCA 
GCAGAACAAG CAGAAGAAAC AGCTCCCATT GAGGCCACTG CAACCAAAGA AGAGGAGGGA 
TCAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHCHD4 ( NM_144636.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_653237.1 Homo sapiens coiled-coil-helix-coiled-coil-helix domain containing 4 (CHCHD4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu17276
Clone ID Related Accession (Same CDS sequence)	NM_144636.2 , NM_144636.3
Accession Version	NM_144636.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Homo sapiens(human)
Product	mitochondrial intermembrane space import and assembly protein 40 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB452249.1, AK056271.1 and BC017082.1. On Jun 6, 2007 this sequence version replaced NM_144636.1. Transcript Variant: This variant (2) contains an alternate segment for the 5' coding region and uses a downstream start codon, compared to variant 1. Variant 2 is found at a much lower frequency than variant 1. The predicted protein (isoform 2) is longer and has a distinct and longer N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK056271.1, SRR1803613.14374.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGCCTGTGT CGTATTCATC TGTGACCACC AGGTACTACC ACAGAGCTGG AGCTGAGGAA 
GGGAAGGATC GAATCATATT TGTAACCAAA GAAGATCATG AAACTCCAAG CAGTGCAGAA 
TTGGTGGCTG ATGACCCCAA CGATCCATAC GAGGAGCATG GATTGATACT GCCAAATGGA 
AACATTAACT GGAACTGCCC ATGCCTTGGG GGAATGGCCA GCGGTCCCTG TGGAGAACAG 
TTTAAGTCAG CCTTTTCCTG CTTCCACTAT AGCACGGAGG AGATCAAGGG GTCAGACTGT 
GTAGACCAGT TCCGGGCCAT GCAGGAATGC ATGCAGAAAT ACCCAGACCT CTATCCCCAA 
GAGGATGAGG ATGAGGAAGA GGAAAGAGAG AAGAAGCCAG CAGAACAAGC AGAAGAAACA 
GCTCCCATTG AGGCCACTGC AACCAAAGAA GAGGAGGGAT CAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_653237.1
CDS	327..794
Translation	MPVSYSSVTTRYYHRAGAEEGKDRIIFVTKEDHETPSSAELVADDPNDPYEEHGLILPNGNINWNCPCLGGMASGPCGEQFKSAFSCFHYSTEEIKGSDCVDQFRAMQECMQKYPDLYPQEDEDEEEEREKKPAEQAEETAPIEATATKEEEGSS

Target ORF information:

RefSeq Version	NM_144636.2
Organism	Homo sapiens(human)
Definition	Homo sapiens coiled-coil-helix-coiled-coil-helix domain containing 4 (CHCHD4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144636.2

ORF Insert Sequence:

ATGCCTGTGT CGTATTCATC TGTGACCACC AGGTACTACC ACAGAGCTGG AGCTGAGGAA 
GGGAAGGATC GAATCATATT TGTAACCAAA GAAGATCATG AAACTCCAAG CAGTGCAGAA 
TTGGTGGCTG ATGACCCCAA CGATCCATAC GAGGAGCATG GATTGATACT GCCAAATGGA 
AACATTAACT GGAACTGCCC ATGCCTTGGG GGAATGGCCA GCGGTCCCTG TGGAGAACAG 
TTTAAGTCAG CCTTTTCCTG CTTCCACTAT AGCACGGAGG AGATCAAGGG GTCAGACTGT 
GTAGACCAGT TCCGGGCCAT GCAGGAATGC ATGCAGAAAT ACCCAGACCT CTATCCCCAA 
GAGGATGAGG ATGAGGAAGA GGAAAGAGAG AAGAAGCCAG CAGAACAAGC AGAAGAAACA 
GCTCCCATTG AGGCCACTGC AACCAAAGAA GAGGAGGGAT CAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHCHD4 ( NM_144636.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_653237.1 Homo sapiens coiled-coil-helix-coiled-coil-helix domain containing 4 (CHCHD4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu17276
Clone ID Related Accession (Same CDS sequence)	NM_144636.2 , NM_144636.3
Accession Version	NM_144636.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	mitochondrial intermembrane space import and assembly protein 40 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB452249.1, AK056271.1 and BC017082.1. On May 31, 2019 this sequence version replaced NM_144636.2. Transcript Variant: This variant (2) contains an alternate segment for the 5' coding region and uses a downstream start codon, compared to variant 1. Variant 2 is found at a much lower frequency than variant 1. The predicted protein (isoform 2) is longer and has a distinct and longer N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.95610.1, SRR3476690.435193.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGCCTGTGT CGTATTCATC TGTGACCACC AGGTACTACC ACAGAGCTGG AGCTGAGGAA 
GGGAAGGATC GAATCATATT TGTAACCAAA GAAGATCATG AAACTCCAAG CAGTGCAGAA 
TTGGTGGCTG ATGACCCCAA CGATCCATAC GAGGAGCATG GATTGATACT GCCAAATGGA 
AACATTAACT GGAACTGCCC ATGCCTTGGG GGAATGGCCA GCGGTCCCTG TGGAGAACAG 
TTTAAGTCAG CCTTTTCCTG CTTCCACTAT AGCACGGAGG AGATCAAGGG GTCAGACTGT 
GTAGACCAGT TCCGGGCCAT GCAGGAATGC ATGCAGAAAT ACCCAGACCT CTATCCCCAA 
GAGGATGAGG ATGAGGAAGA GGAAAGAGAG AAGAAGCCAG CAGAACAAGC AGAAGAAACA 
GCTCCCATTG AGGCCACTGC AACCAAAGAA GAGGAGGGAT CAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_653237.1
CDS	326..793
Translation	MPVSYSSVTTRYYHRAGAEEGKDRIIFVTKEDHETPSSAELVADDPNDPYEEHGLILPNGNINWNCPCLGGMASGPCGEQFKSAFSCFHYSTEEIKGSDCVDQFRAMQECMQKYPDLYPQEDEDEEEEREKKPAEQAEETAPIEATATKEEEGSS

Target ORF information:

RefSeq Version	NM_144636.3
Organism	Homo sapiens(human)
Definition	Homo sapiens coiled-coil-helix-coiled-coil-helix domain containing 4 (CHCHD4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144636.3

ORF Insert Sequence:

ATGCCTGTGT CGTATTCATC TGTGACCACC AGGTACTACC ACAGAGCTGG AGCTGAGGAA 
GGGAAGGATC GAATCATATT TGTAACCAAA GAAGATCATG AAACTCCAAG CAGTGCAGAA 
TTGGTGGCTG ATGACCCCAA CGATCCATAC GAGGAGCATG GATTGATACT GCCAAATGGA 
AACATTAACT GGAACTGCCC ATGCCTTGGG GGAATGGCCA GCGGTCCCTG TGGAGAACAG 
TTTAAGTCAG CCTTTTCCTG CTTCCACTAT AGCACGGAGG AGATCAAGGG GTCAGACTGT 
GTAGACCAGT TCCGGGCCAT GCAGGAATGC ATGCAGAAAT ACCCAGACCT CTATCCCCAA 
GAGGATGAGG ATGAGGAAGA GGAAAGAGAG AAGAAGCCAG CAGAACAAGC AGAAGAAACA 
GCTCCCATTG AGGCCACTGC AACCAAAGAA GAGGAGGGAT CAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Direct structural evidence of protein redox regulation obtained by in-cell NMR.
Biochimica et biophysica acta1863(2)198-204(2016 Feb)
Mercatelli E,Barbieri L,Luchinat E,Banci L

Human mitochondrial MIA40 (CHCHD4) is a component of the Fe-S cluster export machinery.
The Biochemical journal471(2)231-41(2015 Oct)
Murari A,Thiriveedi VR,Mohammad F,Vengaldas V,Gorla M,Tammineni P,Krishnamoorthy T,Sepuri NB

The Ca(2+)-Dependent Release of the Mia40-Induced MICU1-MICU2 Dimer from MCU Regulates Mitochondrial Ca(2+) Uptake.
Cell metabolism22(4)721-33(2015 Oct)
Petrungaro C,Zimmermann KM,K?ttner V,Fischer M,Dengjel J,Bogeski I,Riemer J

Metabolic epistasis among apoptosis-inducing factor and the mitochondrial import factor CHCHD4.
Cell cycle (Georgetown, Tex.)14(17)2743-7(2015)
Modjtahedi N,Hangen E,Gonin P,Kroemer G

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CHCHD4 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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