NTAN1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NTAN1
Entrez Gene ID	123803
Full Name	N-terminal asparagine amidase
Synonyms	PNAA,PNAD
General protein information	Preferred Names N-terminal asparagine amidase Names protein N-terminal asparagine amidohydrolase protein N-terminal Asn amidase protein N-terminal asparagine amidase protein NH2-terminal asparagine deamidase protein NTN-amidase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p13.11
Summary	The protein encoded by this gene functions in a step-wise process of protein degradation through the N-end rule pathway. This protein acts as a tertiary destabilizing enzyme that deamidates N-terminal L-Asn residues on proteins to produce N-terminal L-Asp. L-Asp substrates are subsequently conjugated to L-Arg, which is recognized by specific E3 ubiquitin ligases and targeted to the proteasome. Pseudogenes of this gene are located on the long arms of chromosomes 8, 10 and 12. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012].
Disorder MIM:	615367

mRNA and Protein(s)

mRNA	Protein	Name
NM_001270767.1	NP_001257696.1	protein N-terminal asparagine amidohydrolase isoform 2
XM_011522355.2	XP_011520657.1	protein N-terminal asparagine amidohydrolase isoform X1
NM_001270766.1	NP_001257695.1	protein N-terminal asparagine amidohydrolase isoform 2
NM_173474.4	NP_775745.1	protein N-terminal asparagine amidohydrolase isoform 1
NM_173474.3	NP_775745.1	protein N-terminal asparagine amidohydrolase isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	NTAN1	NP_775745.1
	NTAN1	XP_002802460.1
Danio rerio (zebrafish)	ntan1	XP_005168758.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010963	XP_309738.3
Xenopus tropicalis (tropical clawed frog)	ntan1	NP_001120296.1
Bos taurus (cattle)	NTAN1	NP_001193073.1
Mus musculus (house mouse)	Ntan1	NP_035076.1
Arabidopsis thaliana (thale cress)	AT2G44420	NP_566017.1
Pan troglodytes (chimpanzee)	NTAN1	XP_510838.2
Rattus norvegicus (Norway rat)	Ntan1	NP_001020295.1
Gallus gallus (chicken)	NTAN1	XP_001231313.2
Drosophila melanogaster (fruit fly)	SP2637	NP_611355.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide association study of plasma N6 polyunsaturated fatty acids within the cohorts for heart and aging research in genomic epidemiology consortium.
Guan W, Steffen BT, Lemaitre RN, Wu JHY, Tanaka T, Manichaikul A, Foy M, Rich SS, Wang L, Nettleton JA, Tang W, Gu X, Bandinelli S, King IB, McKnight B, Psaty BM, Siscovick D, Djousse L, Chen YI, Ferrucci L, Fornage M, Mozafarrian D, Tsai MY, Steffen LM
Circulation. Cardiovascular genetics7(3)321-331(2014 Jun)

An atlas of genetic influences on human blood metabolites.
Shin SY, Fauman EB, Petersen AK, Krumsiek J, Santos R, Huang J, Arnold M, Erte I, Forgetta V, Yang TP, Walter K, Menni C, Chen L, Vasquez L, Valdes AM, Hyde CL, Wang V, Ziemek D, Roberts P, Xi L, Grundberg E, , Waldenberger M, Richards JB, Mohney RP, Milburn MV, John SL, Trimmer J, Theis FJ, Overington JP, Suhre K, Brosnan MJ, Gieger C, Kastenm?ller G, Spector TD, Soranzo N
Nature genetics46(6)543-550(2014 Jun)

Expression and biochemical characterization of the human enzyme N-terminal asparagine amidohydrolase.
Cantor JR, Stone EM, Georgiou G
Biochemistry50(14)3025-33(2011 Apr)

Copy number variations of chromosome 16p13.1 region associated with schizophrenia.
Ingason A, Rujescu D, Cichon S, Sigurdsson E, Sigmundsson T, Pietil?inen OP, Buizer-Voskamp JE, Strengman E, Francks C, Muglia P, Gylfason A, Gustafsson O, Olason PI, Steinberg S, Hansen T, Jakobsen KD, Rasmussen HB, Giegling I, M?ller HJ, Hartmann A, Crombie C, Fraser G, Walker N, Lonnqvist J, Suvisaari J, Tuulio-Henriksson A, Bramon E, Kiemeney LA, Franke B, Murray R, Vassos E, Toulopoulou T, M?hleisen TW, Tosato S, Ruggeri M, Djurovic S, Andreassen OA, Zhang Z, Werge T, Ophoff RA, , Rietschel M, N?then MM, Petursson H, Stefansson H, Peltonen L, Collier D, Stefansson K, St Clair DM
Molecular psychiatry16(1)17-25(2011 Jan)

A genome-wide association study in 19 633 Japanese subjects identified LHX3-QSOX2 and IGF1 as adult height loci.
Okada Y, Kamatani Y, Takahashi A, Matsuda K, Hosono N, Ohmiya H, Daigo Y, Yamamoto K, Kubo M, Nakamura Y, Kamatani N
Human molecular genetics19(11)2303-12(2010 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results indicate that hNTAN1 is highly selective for the hydrolysis of N-terminal peptidyl L-Asn.
Title: Expression and biochemical characterization of the human enzyme N-terminal asparagine amidohydrolase.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic predictors of glucocorticoid-induced hypertension in children with acute lymphoblastic leukemia.

The following NTAN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NTAN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03713	NM_001270767.1 Latest version!	Homo sapiens N-terminal asparagine amidase (NTAN1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu03713	NM_001270766.1 Latest version!	Homo sapiens N-terminal asparagine amidase (NTAN1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu07250	NM_173474.4 Latest version!	Homo sapiens N-terminal asparagine amidase (NTAN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu75249	XM_011522355.2 Latest version!	Homo sapiens N-terminal asparagine amidase (NTAN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu07250	NM_173474.3	Homo sapiens N-terminal asparagine amidase (NTAN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NTAN1 ( NM_001270767.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257696.1 Homo sapiens N-terminal asparagine amidase (NTAN1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu03713
Clone ID Related Accession (Same CDS sequence)	NM_001270767.1 , NM_001270766.1
Accession Version	NM_001270767.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	protein N-terminal asparagine amidohydrolase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY036316.1, BF939373.1, BC017336.2 and AF092440.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate in-frame downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. Variants 2 and 3 encode the same isoform (2). ##Evidence-Data-START## Transcript exon combination :: SRR1163658.58665.1, W58767.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2146236 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTCCA TAAAATCCTT TTCTGACCAC GCTCAATGTG GAAGGCTGGA AGTACACCTT 
GTTGGAGGCT TCAGTGACGA CAGGCAGTTG TCACAAAAAC TCACTCATCA ACTTCTTAGT 
GAATTTGACA GGCAAGAAGA TGACATTCAC TTAGTGACAT TATGTGTGAC AGAATTAAAT 
GACCGGGAAG AAAACGAAAA CCACTTTCCA GTAATATATG GCATTGCTGT CAACATTAAG 
ACTGCAGAGA TTTACAGAGC ATCCTTTCAA GATCGGGGTC CGGAGGAGCA GCTTCGTGCT 
GCGCGAACTT TAGCAGGAGG ACCAATGATT AGCATTTATG ATGCAGAGAC AGAACAACTT 
CGTATAGGAC CGTACTCCTG GACACCATTT CCACATGTGG ATTTCTGGTT GCACCAAGAT 
GACAAGCAAA TACTAGAGAA TCTTTCCACT TCGCCTCTGG CTGAGCCACC CCACTTTGTT 
GAACATATTA GATCTACCTT GATGTTTTTA AAAAAACACC CATCTCCAGC TCACACACTG 
TTTTCTGGAA ATAAAGCCCT ACTCTACAAA AAAAATGAAG ATGGCTTGTG GGAAAAGATC 
TCTTCTCCAG GAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257696.1
CDS	255..872
Translation	MNSIKSFSDHAQCGRLEVHLVGGFSDDRQLSQKLTHQLLSEFDRQEDDIHLVTLCVTELNDREENENHFPVIYGIAVNIKTAEIYRASFQDRGPEEQLRAARTLAGGPMISIYDAETEQLRIGPYSWTPFPHVDFWLHQDDKQILENLSTSPLAEPPHFVEHIRSTLMFLKKHPSPAHTLFSGNKALLYKKNEDGLWEKISSPGS

Target ORF information:

RefSeq Version	NM_001270767.1
Organism	Homo sapiens(human)
Definition	Homo sapiens N-terminal asparagine amidase (NTAN1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270767.1

ORF Insert Sequence:

ATGAACTCCA TAAAATCCTT TTCTGACCAC GCTCAATGTG GAAGGCTGGA AGTACACCTT 
GTTGGAGGCT TCAGTGACGA CAGGCAGTTG TCACAAAAAC TCACTCATCA ACTTCTTAGT 
GAATTTGACA GGCAAGAAGA TGACATTCAC TTAGTGACAT TATGTGTGAC AGAATTAAAT 
GACCGGGAAG AAAACGAAAA CCACTTTCCA GTAATATATG GCATTGCTGT CAACATTAAG 
ACTGCAGAGA TTTACAGAGC ATCCTTTCAA GATCGGGGTC CGGAGGAGCA GCTTCGTGCT 
GCGCGAACTT TAGCAGGAGG ACCAATGATT AGCATTTATG ATGCAGAGAC AGAACAACTT 
CGTATAGGAC CGTACTCCTG GACACCATTT CCACATGTGG ATTTCTGGTT GCACCAAGAT 
GACAAGCAAA TACTAGAGAA TCTTTCCACT TCGCCTCTGG CTGAGCCACC CCACTTTGTT 
GAACATATTA GATCTACCTT GATGTTTTTA AAAAAACACC CATCTCCAGC TCACACACTG 
TTTTCTGGAA ATAAAGCCCT ACTCTACAAA AAAAATGAAG ATGGCTTGTG GGAAAAGATC 
TCTTCTCCAG GAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NTAN1 ( NM_001270766.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257695.1 Homo sapiens N-terminal asparagine amidase (NTAN1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu03713
Clone ID Related Accession (Same CDS sequence)	NM_001270767.1 , NM_001270766.1
Accession Version	NM_001270766.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-03
Organism	Homo sapiens(human)
Product	protein N-terminal asparagine amidohydrolase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY036316.1, DC383993.1, BC017336.2 and AF092440.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate in-frame downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. Variants 2 and 3 encode the same isoform (2). ##Evidence-Data-START## Transcript exon combination :: SRR5189655.179388.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2146236 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTCCA TAAAATCCTT TTCTGACCAC GCTCAATGTG GAAGGCTGGA AGTACACCTT 
GTTGGAGGCT TCAGTGACGA CAGGCAGTTG TCACAAAAAC TCACTCATCA ACTTCTTAGT 
GAATTTGACA GGCAAGAAGA TGACATTCAC TTAGTGACAT TATGTGTGAC AGAATTAAAT 
GACCGGGAAG AAAACGAAAA CCACTTTCCA GTAATATATG GCATTGCTGT CAACATTAAG 
ACTGCAGAGA TTTACAGAGC ATCCTTTCAA GATCGGGGTC CGGAGGAGCA GCTTCGTGCT 
GCGCGAACTT TAGCAGGAGG ACCAATGATT AGCATTTATG ATGCAGAGAC AGAACAACTT 
CGTATAGGAC CGTACTCCTG GACACCATTT CCACATGTGG ATTTCTGGTT GCACCAAGAT 
GACAAGCAAA TACTAGAGAA TCTTTCCACT TCGCCTCTGG CTGAGCCACC CCACTTTGTT 
GAACATATTA GATCTACCTT GATGTTTTTA AAAAAACACC CATCTCCAGC TCACACACTG 
TTTTCTGGAA ATAAAGCCCT ACTCTACAAA AAAAATGAAG ATGGCTTGTG GGAAAAGATC 
TCTTCTCCAG GAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257695.1
CDS	321..938
Translation	MNSIKSFSDHAQCGRLEVHLVGGFSDDRQLSQKLTHQLLSEFDRQEDDIHLVTLCVTELNDREENENHFPVIYGIAVNIKTAEIYRASFQDRGPEEQLRAARTLAGGPMISIYDAETEQLRIGPYSWTPFPHVDFWLHQDDKQILENLSTSPLAEPPHFVEHIRSTLMFLKKHPSPAHTLFSGNKALLYKKNEDGLWEKISSPGS

Target ORF information:

RefSeq Version	NM_001270766.1
Organism	Homo sapiens(human)
Definition	Homo sapiens N-terminal asparagine amidase (NTAN1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270766.1

ORF Insert Sequence:

ATGAACTCCA TAAAATCCTT TTCTGACCAC GCTCAATGTG GAAGGCTGGA AGTACACCTT 
GTTGGAGGCT TCAGTGACGA CAGGCAGTTG TCACAAAAAC TCACTCATCA ACTTCTTAGT 
GAATTTGACA GGCAAGAAGA TGACATTCAC TTAGTGACAT TATGTGTGAC AGAATTAAAT 
GACCGGGAAG AAAACGAAAA CCACTTTCCA GTAATATATG GCATTGCTGT CAACATTAAG 
ACTGCAGAGA TTTACAGAGC ATCCTTTCAA GATCGGGGTC CGGAGGAGCA GCTTCGTGCT 
GCGCGAACTT TAGCAGGAGG ACCAATGATT AGCATTTATG ATGCAGAGAC AGAACAACTT 
CGTATAGGAC CGTACTCCTG GACACCATTT CCACATGTGG ATTTCTGGTT GCACCAAGAT 
GACAAGCAAA TACTAGAGAA TCTTTCCACT TCGCCTCTGG CTGAGCCACC CCACTTTGTT 
GAACATATTA GATCTACCTT GATGTTTTTA AAAAAACACC CATCTCCAGC TCACACACTG 
TTTTCTGGAA ATAAAGCCCT ACTCTACAAA AAAAATGAAG ATGGCTTGTG GGAAAAGATC 
TCTTCTCCAG GAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NTAN1 ( NM_173474.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_775745.1 Homo sapiens N-terminal asparagine amidase (NTAN1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu07250
Clone ID Related Accession (Same CDS sequence)	NM_173474.4 , NM_173474.3
Accession Version	NM_173474.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	protein N-terminal asparagine amidohydrolase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY036316.1 and BC017336.2. On Nov 24, 2018 this sequence version replaced NM_173474.3. Transcript Variant: This variant (1) represents the longesr transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1163657.574757.1, SRR1163655.137300.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2146236 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000287706.8/ ENSP00000287706.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGCTGC TCGTCGAGGG GCGGCGAGTG CGGCTGCCGC AGTCAGCCGG GGACCTCGTC 
CGAGCCCACC CGCCTTTGGA GGAAAGAGCC AGACTTCTCA GAGGTCAGTC TGTTCAACAA 
GTGGGACCCC AGGGCCTTCT GTATGTTCAG CAAAGAGAGC TTGCAGTGAC CTCCCCAAAG 
GATGGCTCCA TCTCCATTCT GGGTTCTGAT GATGCCACTA CTTGTCACAT TGTGGTCCTG 
AGGCACACAG GTAATGGGGC CACCTGCTTG ACACATTGTG ACGGAACCGA CACCAAAGCT 
GAGGTCCCCT TGATCATGAA CTCCATAAAA TCCTTTTCTG ACCACGCTCA ATGTGGAAGG 
CTGGAAGTAC ACCTTGTTGG AGGCTTCAGT GACGACAGGC AGTTGTCACA AAAACTCACT 
CATCAACTTC TTAGTGAATT TGACAGGCAA GAAGATGACA TTCACTTAGT GACATTATGT 
GTGACAGAAT TAAATGACCG GGAAGAAAAC GAAAACCACT TTCCAGTAAT ATATGGCATT 
GCTGTCAACA TTAAGACTGC AGAGATTTAC AGAGCATCCT TTCAAGATCG GGGTCCGGAG 
GAGCAGCTTC GTGCTGCGCG AACTTTAGCA GGAGGACCAA TGATTAGCAT TTATGATGCA 
GAGACAGAAC AACTTCGTAT AGGACCGTAC TCCTGGACAC CATTTCCACA TGTGGATTTC 
TGGTTGCACC AAGATGACAA GCAAATACTA GAGAATCTTT CCACTTCGCC TCTGGCTGAG 
CCACCCCACT TTGTTGAACA TATTAGATCT ACCTTGATGT TTTTAAAAAA ACACCCATCT 
CCAGCTCACA CACTGTTTTC TGGAAATAAA GCCCTACTCT ACAAAAAAAA TGAAGATGGC 
TTGTGGGAAA AGATCTCTTC TCCAGGAAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775745.1
CDS	104..1036
Translation	MPLLVEGRRVRLPQSAGDLVRAHPPLEERARLLRGQSVQQVGPQGLLYVQQRELAVTSPKDGSISILGSDDATTCHIVVLRHTGNGATCLTHCDGTDTKAEVPLIMNSIKSFSDHAQCGRLEVHLVGGFSDDRQLSQKLTHQLLSEFDRQEDDIHLVTLCVTELNDREENENHFPVIYGIAVNIKTAEIYRASFQDRGPEEQLRAARTLAGGPMISIYDAETEQLRIGPYSWTPFPHVDFWLHQDDKQILENLSTSPLAEPPHFVEHIRSTLMFLKKHPSPAHTLFSGNKALLYKKNEDGLWEKISSPGS

Target ORF information:

RefSeq Version	NM_173474.4
Organism	Homo sapiens(human)
Definition	Homo sapiens N-terminal asparagine amidase (NTAN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173474.4

ORF Insert Sequence:

ATGCCGCTGC TCGTCGAGGG GCGGCGAGTG CGGCTGCCGC AGTCAGCCGG GGACCTCGTC 
CGAGCCCACC CGCCTTTGGA GGAAAGAGCC AGACTTCTCA GAGGTCAGTC TGTTCAACAA 
GTGGGACCCC AGGGCCTTCT GTATGTTCAG CAAAGAGAGC TTGCAGTGAC CTCCCCAAAG 
GATGGCTCCA TCTCCATTCT GGGTTCTGAT GATGCCACTA CTTGTCACAT TGTGGTCCTG 
AGGCACACAG GTAATGGGGC CACCTGCTTG ACACATTGTG ACGGAACCGA CACCAAAGCT 
GAGGTCCCCT TGATCATGAA CTCCATAAAA TCCTTTTCTG ACCACGCTCA ATGTGGAAGG 
CTGGAAGTAC ACCTTGTTGG AGGCTTCAGT GACGACAGGC AGTTGTCACA AAAACTCACT 
CATCAACTTC TTAGTGAATT TGACAGGCAA GAAGATGACA TTCACTTAGT GACATTATGT 
GTGACAGAAT TAAATGACCG GGAAGAAAAC GAAAACCACT TTCCAGTAAT ATATGGCATT 
GCTGTCAACA TTAAGACTGC AGAGATTTAC AGAGCATCCT TTCAAGATCG GGGTCCGGAG 
GAGCAGCTTC GTGCTGCGCG AACTTTAGCA GGAGGACCAA TGATTAGCAT TTATGATGCA 
GAGACAGAAC AACTTCGTAT AGGACCGTAC TCCTGGACAC CATTTCCACA TGTGGATTTC 
TGGTTGCACC AAGATGACAA GCAAATACTA GAGAATCTTT CCACTTCGCC TCTGGCTGAG 
CCACCCCACT TTGTTGAACA TATTAGATCT ACCTTGATGT TTTTAAAAAA ACACCCATCT 
CCAGCTCACA CACTGTTTTC TGGAAATAAA GCCCTACTCT ACAAAAAAAA TGAAGATGGC 
TTGTGGGAAA AGATCTCTTC TCCAGGAAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NTAN1 ( NM_173474.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_775745.1 Homo sapiens N-terminal asparagine amidase (NTAN1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu07250
Clone ID Related Accession (Same CDS sequence)	NM_173474.4 , NM_173474.3
Accession Version	NM_173474.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	protein N-terminal asparagine amidohydrolase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY036316.1, BC017336.2 and AF092440.1. On Aug 1, 2012 this sequence version replaced NM_173474.2. Transcript Variant: This variant (1) represents the longesr transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AF092440.1, SRR1163655.137300.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2146236 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGCTGC TCGTCGAGGG GCGGCGAGTG CGGCTGCCGC AGTCAGCCGG GGACCTCGTC 
CGAGCCCACC CGCCTTTGGA GGAAAGAGCC AGACTTCTCA GAGGTCAGTC TGTTCAACAA 
GTGGGACCCC AGGGCCTTCT GTATGTTCAG CAAAGAGAGC TTGCAGTGAC CTCCCCAAAG 
GATGGCTCCA TCTCCATTCT GGGTTCTGAT GATGCCACTA CTTGTCACAT TGTGGTCCTG 
AGGCACACAG GTAATGGGGC CACCTGCTTG ACACATTGTG ACGGAACCGA CACCAAAGCT 
GAGGTCCCCT TGATCATGAA CTCCATAAAA TCCTTTTCTG ACCACGCTCA ATGTGGAAGG 
CTGGAAGTAC ACCTTGTTGG AGGCTTCAGT GACGACAGGC AGTTGTCACA AAAACTCACT 
CATCAACTTC TTAGTGAATT TGACAGGCAA GAAGATGACA TTCACTTAGT GACATTATGT 
GTGACAGAAT TAAATGACCG GGAAGAAAAC GAAAACCACT TTCCAGTAAT ATATGGCATT 
GCTGTCAACA TTAAGACTGC AGAGATTTAC AGAGCATCCT TTCAAGATCG GGGTCCGGAG 
GAGCAGCTTC GTGCTGCGCG AACTTTAGCA GGAGGACCAA TGATTAGCAT TTATGATGCA 
GAGACAGAAC AACTTCGTAT AGGACCGTAC TCCTGGACAC CATTTCCACA TGTGGATTTC 
TGGTTGCACC AAGATGACAA GCAAATACTA GAGAATCTTT CCACTTCGCC TCTGGCTGAG 
CCACCCCACT TTGTTGAACA TATTAGATCT ACCTTGATGT TTTTAAAAAA ACACCCATCT 
CCAGCTCACA CACTGTTTTC TGGAAATAAA GCCCTACTCT ACAAAAAAAA TGAAGATGGC 
TTGTGGGAAA AGATCTCTTC TCCAGGAAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775745.1
CDS	109..1041
Translation	MPLLVEGRRVRLPQSAGDLVRAHPPLEERARLLRGQSVQQVGPQGLLYVQQRELAVTSPKDGSISILGSDDATTCHIVVLRHTGNGATCLTHCDGTDTKAEVPLIMNSIKSFSDHAQCGRLEVHLVGGFSDDRQLSQKLTHQLLSEFDRQEDDIHLVTLCVTELNDREENENHFPVIYGIAVNIKTAEIYRASFQDRGPEEQLRAARTLAGGPMISIYDAETEQLRIGPYSWTPFPHVDFWLHQDDKQILENLSTSPLAEPPHFVEHIRSTLMFLKKHPSPAHTLFSGNKALLYKKNEDGLWEKISSPGS

Target ORF information:

RefSeq Version	NM_173474.3
Organism	Homo sapiens(human)
Definition	Homo sapiens N-terminal asparagine amidase (NTAN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173474.3

ORF Insert Sequence:

ATGCCGCTGC TCGTCGAGGG GCGGCGAGTG CGGCTGCCGC AGTCAGCCGG GGACCTCGTC 
CGAGCCCACC CGCCTTTGGA GGAAAGAGCC AGACTTCTCA GAGGTCAGTC TGTTCAACAA 
GTGGGACCCC AGGGCCTTCT GTATGTTCAG CAAAGAGAGC TTGCAGTGAC CTCCCCAAAG 
GATGGCTCCA TCTCCATTCT GGGTTCTGAT GATGCCACTA CTTGTCACAT TGTGGTCCTG 
AGGCACACAG GTAATGGGGC CACCTGCTTG ACACATTGTG ACGGAACCGA CACCAAAGCT 
GAGGTCCCCT TGATCATGAA CTCCATAAAA TCCTTTTCTG ACCACGCTCA ATGTGGAAGG 
CTGGAAGTAC ACCTTGTTGG AGGCTTCAGT GACGACAGGC AGTTGTCACA AAAACTCACT 
CATCAACTTC TTAGTGAATT TGACAGGCAA GAAGATGACA TTCACTTAGT GACATTATGT 
GTGACAGAAT TAAATGACCG GGAAGAAAAC GAAAACCACT TTCCAGTAAT ATATGGCATT 
GCTGTCAACA TTAAGACTGC AGAGATTTAC AGAGCATCCT TTCAAGATCG GGGTCCGGAG 
GAGCAGCTTC GTGCTGCGCG AACTTTAGCA GGAGGACCAA TGATTAGCAT TTATGATGCA 
GAGACAGAAC AACTTCGTAT AGGACCGTAC TCCTGGACAC CATTTCCACA TGTGGATTTC 
TGGTTGCACC AAGATGACAA GCAAATACTA GAGAATCTTT CCACTTCGCC TCTGGCTGAG 
CCACCCCACT TTGTTGAACA TATTAGATCT ACCTTGATGT TTTTAAAAAA ACACCCATCT 
CCAGCTCACA CACTGTTTTC TGGAAATAAA GCCCTACTCT ACAAAAAAAA TGAAGATGGC 
TTGTGGGAAA AGATCTCTTC TCCAGGAAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NTAN1 ( XM_011522355.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520657.1 Homo sapiens N-terminal asparagine amidase (NTAN1), transcript variant X1, mRNA.

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CloneID	OHu75249
Clone ID Related Accession (Same CDS sequence)	XM_011522355.2
Accession Version	XM_011522355.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein N-terminal asparagine amidohydrolase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522355.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTGGA GCACACAGGA GCTAGGTGGA GGCCGACGAC ACAGGCCTGC TGTCAAGACC 
ACTGTTCACA CACCTGTCCC CTCTCCTTGC TCTTTGTGGA GACCTGGCAG GCTGGAAGTA 
CACCTTGTTG GAGGCTTCAG TGACGACAGG CAGTTGTCAC AAAAACTCAC TCATCAACTT 
CTTAGTGAAT TTGACAGGCA AGAAGATGAC ATTCACTTAG TGACATTATG TGTGACAGAA 
TTAAATGACC GGGAAGAAAA CGAAAACCAC TTTCCAGTAA TATATGGCAT TGCTGTCAAC 
ATTAAGACTG CAGAGATTTA CAGAGCATCC TTTCAAGATC GGGGTCCGGA GGAGCAGCTT 
CGTGCTGCGC GAACTTTAGC AGGAGGACCA ATGATTAGCA TTTATGATGC AGAGACAGAA 
CAACTTCGTA TAGGACCGTA CTCCTGGACA CCATTTCCAC ATGTGGATTT CTGGTTGCAC 
CAAGATGACA AGCAAATACT AGAGAATCTT TCCACTTCGC CTCTGGCTGA GCCACCCCAC 
TTTGTTGAAC ATATTAGATC TACCTTGATG TTTTTAAAAA AACACCCATC TCCAGCTCAC 
ACACTGTTTT CTGGAAATAA AGCCCTACTC TACAAAAAAA ATGAAGATGG CTTGTGGGAA 
AAGATCTCTT CTCCAGGAAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011520657.1
CDS	193..876
Translation	MCWSTQELGGGRRHRPAVKTTVHTPVPSPCSLWRPGRLEVHLVGGFSDDRQLSQKLTHQLLSEFDRQEDDIHLVTLCVTELNDREENENHFPVIYGIAVNIKTAEIYRASFQDRGPEEQLRAARTLAGGPMISIYDAETEQLRIGPYSWTPFPHVDFWLHQDDKQILENLSTSPLAEPPHFVEHIRSTLMFLKKHPSPAHTLFSGNKALLYKKNEDGLWEKISSPGS

Target ORF information:

RefSeq Version	XM_011522355.2
Organism	Homo sapiens(human)
Definition	Homo sapiens N-terminal asparagine amidase (NTAN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522355.2

ORF Insert Sequence:

ATGTGCTGGA GCACACAGGA GCTAGGTGGA GGCCGACGAC ACAGGCCTGC TGTCAAGACC 
ACTGTTCACA CACCTGTCCC CTCTCCTTGC TCTTTGTGGA GACCTGGCAG GCTGGAAGTA 
CACCTTGTTG GAGGCTTCAG TGACGACAGG CAGTTGTCAC AAAAACTCAC TCATCAACTT 
CTTAGTGAAT TTGACAGGCA AGAAGATGAC ATTCACTTAG TGACATTATG TGTGACAGAA 
TTAAATGACC GGGAAGAAAA CGAAAACCAC TTTCCAGTAA TATATGGCAT TGCTGTCAAC 
ATTAAGACTG CAGAGATTTA CAGAGCATCC TTTCAAGATC GGGGTCCGGA GGAGCAGCTT 
CGTGCTGCGC GAACTTTAGC AGGAGGACCA ATGATTAGCA TTTATGATGC AGAGACAGAA 
CAACTTCGTA TAGGACCGTA CTCCTGGACA CCATTTCCAC ATGTGGATTT CTGGTTGCAC 
CAAGATGACA AGCAAATACT AGAGAATCTT TCCACTTCGC CTCTGGCTGA GCCACCCCAC 
TTTGTTGAAC ATATTAGATC TACCTTGATG TTTTTAAAAA AACACCCATC TCCAGCTCAC 
ACACTGTTTT CTGGAAATAA AGCCCTACTC TACAAAAAAA ATGAAGATGG CTTGTGGGAA 
AAGATCTCTT CTCCAGGAAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide association study of plasma N6 polyunsaturated fatty acids within the cohorts for heart and aging research in genomic epidemiology consortium.
Circulation. Cardiovascular genetics7(3)321-331(2014 Jun)
Guan W,Steffen BT,Lemaitre RN,Wu JHY,Tanaka T,Manichaikul A,Foy M,Rich SS,Wang L,Nettleton JA,Tang W,Gu X,Bandinelli S,King IB,McKnight B,Psaty BM,Siscovick D,Djousse L,Chen YI,Ferrucci L,Fornage M,Mozafarrian D,Tsai MY,Steffen LM

An atlas of genetic influences on human blood metabolites.
Nature genetics46(6)543-550(2014 Jun)
Shin SY,Fauman EB,Petersen AK,Krumsiek J,Santos R,Huang J,Arnold M,Erte I,Forgetta V,Yang TP,Walter K,Menni C,Chen L,Vasquez L,Valdes AM,Hyde CL,Wang V,Ziemek D,Roberts P,Xi L,Grundberg E,,Waldenberger M,Richards JB,Mohney RP,Milburn MV,John SL,Trimmer J,Theis FJ,Overington JP,Suhre K,Brosnan MJ,Gieger C,Kastenm?ller G,Spector TD,Soranzo N

Expression and biochemical characterization of the human enzyme N-terminal asparagine amidohydrolase.
Biochemistry50(14)3025-33(2011 Apr)
Cantor JR,Stone EM,Georgiou G

Copy number variations of chromosome 16p13.1 region associated with schizophrenia.
Molecular psychiatry16(1)17-25(2011 Jan)
Ingason A,Rujescu D,Cichon S,Sigurdsson E,Sigmundsson T,Pietil?inen OP,Buizer-Voskamp JE,Strengman E,Francks C,Muglia P,Gylfason A,Gustafsson O,Olason PI,Steinberg S,Hansen T,Jakobsen KD,Rasmussen HB,Giegling I,M?ller HJ,Hartmann A,Crombie C,Fraser G,Walker N,Lonnqvist J,Suvisaari J,Tuulio-Henriksson A,Bramon E,Kiemeney LA,Franke B,Murray R,Vassos E,Toulopoulou T,M?hleisen TW,Tosato S,Ruggeri M,Djurovic S,Andreassen OA,Zhang Z,Werge T,Ophoff RA,,Rietschel M,N?then MM,Petursson H,Stefansson H,Peltonen L,Collier D,Stefansson K,St Clair DM

A genome-wide association study in 19 633 Japanese subjects identified LHX3-QSOX2 and IGF1 as adult height loci.
Human molecular genetics19(11)2303-12(2010 Jun)
Okada Y,Kamatani Y,Takahashi A,Matsuda K,Hosono N,Ohmiya H,Daigo Y,Yamamoto K,Kubo M,Nakamura Y,Kamatani N

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NTAN1 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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