STON1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	STON1
Entrez Gene ID	11037
Full Name	stonin 1
Synonyms	SALF,SBLF,STN1,STNB1
General protein information	Preferred Names stonin 1 Names stonin-1 stoned B homolog 1 stoned B-like factor stoned-b1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p16.3
Summary	Endocytosis of cell surface proteins is mediated by a complex molecular machinery that assembles on the inner surface of the plasma membrane. This gene encodes one of two human homologs of the Drosophila melanogaster stoned B protein. This protein is related to components of the endocytic machinery and exhibits a modular structure consisting of an N-terminal proline-rich domain, a central region of homology specific to the human stoned B-like proteins, and a C-terminal region homologous to the mu subunits of adaptor protein (AP) complexes. Read-through transcription of this gene into the neighboring downstream gene, which encodes TFIIA-alpha/beta-like factor, generates a transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2010].
Disorder MIM:	605357

mRNA and Protein(s)

mRNA	Protein	Name
NM_006873.3	NP_006864.2	stonin-1
NM_001198595.1	NP_001185524.1	stonin-1

Pathways from BioSystems

Homologies

Homo sapiens (human)	STON1	NP_006864.2
Canis lupus familiaris (dog)	STON1	NP_001191771.1
Bos taurus (cattle)	STON1	NP_001191780.1
Rattus norvegicus (Norway rat)	Ston1	NP_891999.2
Mus musculus (house mouse)	Ston1	NP_084134.2
Gallus gallus (chicken)	STON1	NP_001191794.2
Xenopus tropicalis (tropical clawed frog)	ston1	NP_001191796.1
Pan troglodytes (chimpanzee)	STON1	NP_001191754.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide association study combining pathway analysis for typical sporadic amyotrophic lateral sclerosis in Chinese Han populations.
Xie T, Deng L, Mei P, Zhou Y, Wang B, Zhang J, Lin J, Wei Y, Zhang X, Xu R
Neurobiology of aging35(7)1778.e9-1778.e23(2014 Jul)

Stonins--specialized adaptors for synaptic vesicle recycling and beyond?
Maritzen T, Podufall J, Haucke V
Traffic (Copenhagen, Denmark)11(1)8-15(2010 Jan)

Functional dissection of a eukaryotic dicistronic gene: transgenic stonedB, but not stonedA, restores normal synaptic properties to Drosophila stoned mutants.
Estes PS, Jackson TC, Stimson DT, Sanyal S, Kelly LE, Ramaswami M
Genetics165(1)185-96(2003 Sep)

Human stoned B interacts with AP-2 and synaptotagmin and facilitates clathrin-coated vesicle uncoating.
Walther K, Krauss M, Diril MK, Lemke S, Ricotta D, Honing S, Kaiser S, Haucke V
EMBO reports2(7)634-40(2001 Jul)

Stonin 2: an adaptor-like protein that interacts with components of the endocytic machinery.
Martina JA, Bonangelino CJ, Aguilar RC, Bonifacino JS
The Journal of cell biology153(5)1111-20(2001 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Functional analysis of an homologous Drosophila gene.
Title: Functional dissection of a eukaryotic dicistronic gene: transgenic stonedB, but not stonedA, restores normal synaptic properties to Drosophila stoned mutants.

The following STON1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the STON1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu10282	NM_006873.3 Latest version!	Homo sapiens stonin 1 (STON1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu10282	NM_001198595.1 Latest version!	Homo sapiens stonin 1 (STON1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

STON1 ( NM_006873.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006864.2 Homo sapiens stonin 1 (STON1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10282
Clone ID Related Accession (Same CDS sequence)	NM_006873.3 , NM_001198595.1
Accession Version	NM_006873.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-01
Organism	Homo sapiens(human)
Product	stonin-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK314464.1, AF255310.1, AC073082.6 and BM974074.1. On Oct 29, 2010 this sequence version replaced NM_006873.2. Transcript Variant: This variant (2) lacks an alternate exon in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK314464.1, SRR1660805.210850.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGCTCCA CAAATCCAGG CAAATGGGTC ACCTTTGATG ATGATCCTGC TGTTCAATCT 
TCTCAAAAGT CAAAGAATTT TCCTCTGGAG AATCAAGGTG TCTGTAGACC AAATGGACTG 
AAGCTGAACC TTCCTGGCCT CAGGGAATTT CCCAGTGGAT CTTCCTCCAC CAGCAGCACT 
CCTCTCTCCT CCCCCATTGT AGATTTTTAT TTCAGTCCAG GACCTCCAAG TAACTCTCCT 
CTTTCTACAC CTACCAAAGA CTTCCCAGGT TTTCCTGGCA TCCCCAAAGC AGGGACTCAT 
GTGCTTTATC CTATTCCAGA ATCATCTTCA GACAGCCCAC TCGCAATATC AGGAGGAGAA 
TCTTCCTTAC TGCCTACCAG ACCAACATGT TTATCCCATG CCTTGTTACC CAGTGACCAC 
TCATGTACAC ATCCAACTCC CAAAGTAGGT CTTCCAGATG AAGTTAATCC TCAACAGGCT 
GAAAGCCTAG GATTCCAAAG TGATGATCTC CCCCAGTTTC AGTATTTTCG AGAGGACTGT 
GCTTTTTCAA GTCCATTTTG GAAAGATGAA GGCAGTGATT CCCATTTCAC CCTTGACCCA 
CCAGGAAGCA AAAAGATGTT CTCATCAAGA AACAAGGAGA TGCCTATTGA CCAAAAAAGC 
CTAAATAAGT GTTCACTCAA CTATATCTGT GAGAAGCTTG AACATCTCCA GTCAGCTGAG 
AACCAAGACT CACTTAGAAG TTTGTCTATG CACTGTCTAT GTGCTGAAGA AAATGCCTCT 
TCCTTTGTCC CCCACACACT CTTCAGGAGT CAGCCAAAAT CCGGATGGTC TTTCATGCTG 
AGAATTCCTG AGAAGAAGAA TATGATGTCT TCCCGGCAAT GGGGACCAAT TTTTCTGAAA 
GTTTTGCCTG GAGGAATTTT GCAGATGTAT TATGAACAGG GATTAGAAAA ACCATTTAAA 
GAGATACAGC TTGATCCATA TTGTAGGCTT TCTGAACCCA AGGTTGAGAA CTTCAGTGTA 
GCAGGAAAAA TCCACACTGT GAAGATTGAA CATGTGTCTT ACACAGAAAA AAGGAAATAC 
CATTCTAAGA CAGAAGTAGT TCATGAACCT GACATAGAGC AGATGCTGAA GTTGGGGTCC 
ACATCGTACC ATGACTTCCT TGACTTTCTG ACTACTGTGG AGGAGGAGCT GATGAAGTTG 
CCAGCTGTTT CAAAACCAAA AAAGAACTAC GAGGAGCAAG AAATTTCCTT GGAAATTGTG 
GACAACTTTT GGGGTAAAGT CACAAAAGAA GGAAAATTTG TTGAAAGTGC TGTGATAACT 
CAAATTTATT GCCTCTGCTT TGTGAATGGG AACCTGGAAT GCTTTTTAAC CTTGAATGAC 
CTTGAGTTGC CGAAGCGAGA TGAATCCTAT TATGAGAAGG ACTCAGAAAA AAAGGGGATT 
GATATTCTTG ACTACCATTT TCATAAGTGT GTGAATGTAC AAGAATTTGA GCAATCAAGA 
ATCATTAAGT TTGTACCTCT GGATGCCTGC CGGTTTGAGC TGATGCGTTT CAAGACTTTG 
TATAATGGGG ATAATCTTCC CTTTTCCTTG AAGTCTGTAG TGGTTGTCCA GGGAGCATAC 
GTGGAACTTC AGGCTTTTGT CAACATGGCC TCATTGGCGC AGAGGTCATC CTATGCTGGT 
TCCTTAAGGT CCTGTGACAA TATAAGGATA CACTTTCCTG TCCCATCGCA GTGGATCAAG 
GCCCTTTGGA CCATGAACCT CCAGAGGCAG AAGTCTCTGA AAGCTAAAAT GAACCGCCGA 
GCATGTCTGG GGAGTTTACA GGAACTTGAA TCTGAACCTG TCATTCAAGT CACTGTGGGG 
TCAGCAAAAT ATGAGAGTGC CTACCAGGCA GTGGTATGGA AGATAGATCG GCTTCCAGAC 
AAAAATTCAA GTCTAGATCA TCCCCATTGT CTGTCATACA AATTAGAGCT TGGATCAGAC 
CAAGAAATTC CCTCTGATTG GTATCCATTT GCTACTGTTC AGTTTTCCGT GCCTGACACC 
TGTGCCTCAA GGACAGAGGT CAGGTCTCTG GGAGTGGAGA GTGATGTCCA GCCACAGAAA 
CATGTTCAGC AGCGAGCTTG CTACAACATC CAGGTTGAAA TAGAAAAGAA GTGGATTAAA 
ATCGATGGAG AAGACCCAGA TAAAATTGGT GACTGCATAA CTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006864.2
CDS	96..2303
Translation	MCSTNPGKWVTFDDDPAVQSSQKSKNFPLENQGVCRPNGLKLNLPGLREFPSGSSSTSSTPLSSPIVDFYFSPGPPSNSPLSTPTKDFPGFPGIPKAGTHVLYPIPESSSDSPLAISGGESSLLPTRPTCLSHALLPSDHSCTHPTPKVGLPDEVNPQQAESLGFQSDDLPQFQYFREDCAFSSPFWKDEGSDSHFTLDPPGSKKMFSSRNKEMPIDQKSLNKCSLNYICEKLEHLQSAENQDSLRSLSMHCLCAEENASSFVPHTLFRSQPKSGWSFMLRIPEKKNMMSSRQWGPIFLKVLPGGILQMYYEQGLEKPFKEIQLDPYCRLSEPKVENFSVAGKIHTVKIEHVSYTEKRKYHSKTEVVHEPDIEQMLKLGSTSYHDFLDFLTTVEEELMKLPAVSKPKKNYEEQEISLEIVDNFWGKVTKEGKFVESAVITQIYCLCFVNGNLECFLTLNDLELPKRDESYYEKDSEKKGIDILDYHFHKCVNVQEFEQSRIIKFVPLDACRFELMRFKTLYNGDNLPFSLKSVVVVQGAYVELQAFVNMASLAQRSSYAGSLRSCDNIRIHFPVPSQWIKALWTMNLQRQKSLKAKMNRRACLGSLQELESEPVIQVTVGSAKYESAYQAVVWKIDRLPDKNSSLDHPHCLSYKLELGSDQEIPSDWYPFATVQFSVPDTCASRTEVRSLGVESDVQPQKHVQQRACYNIQVEIEKKWIKIDGEDPDKIGDCITQ

Target ORF information:

RefSeq Version	NM_006873.3
Organism	Homo sapiens(human)
Definition	Homo sapiens stonin 1 (STON1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006873.3

ORF Insert Sequence:

ATGTGCTCCA CAAATCCAGG CAAATGGGTC ACCTTTGATG ATGATCCTGC TGTTCAATCT 
TCTCAAAAGT CAAAGAATTT TCCTCTGGAG AATCAAGGTG TCTGTAGACC AAATGGACTG 
AAGCTGAACC TTCCTGGCCT CAGGGAATTT CCCAGTGGAT CTTCCTCCAC CAGCAGCACT 
CCTCTCTCCT CCCCCATTGT AGATTTTTAT TTCAGTCCAG GACCTCCAAG TAACTCTCCT 
CTTTCTACAC CTACCAAAGA CTTCCCAGGT TTTCCTGGCA TCCCCAAAGC AGGGACTCAT 
GTGCTTTATC CTATTCCAGA ATCATCTTCA GACAGCCCAC TCGCAATATC AGGAGGAGAA 
TCTTCCTTAC TGCCTACCAG ACCAACATGT TTATCCCATG CCTTGTTACC CAGTGACCAC 
TCATGTACAC ATCCAACTCC CAAAGTAGGT CTTCCAGATG AAGTTAATCC TCAACAGGCT 
GAAAGCCTAG GATTCCAAAG TGATGATCTC CCCCAGTTTC AGTATTTTCG AGAGGACTGT 
GCTTTTTCAA GTCCATTTTG GAAAGATGAA GGCAGTGATT CCCATTTCAC CCTTGACCCA 
CCAGGAAGCA AAAAGATGTT CTCATCAAGA AACAAGGAGA TGCCTATTGA CCAAAAAAGC 
CTAAATAAGT GTTCACTCAA CTATATCTGT GAGAAGCTTG AACATCTCCA GTCAGCTGAG 
AACCAAGACT CACTTAGAAG TTTGTCTATG CACTGTCTAT GTGCTGAAGA AAATGCCTCT 
TCCTTTGTCC CCCACACACT CTTCAGGAGT CAGCCAAAAT CCGGATGGTC TTTCATGCTG 
AGAATTCCTG AGAAGAAGAA TATGATGTCT TCCCGGCAAT GGGGACCAAT TTTTCTGAAA 
GTTTTGCCTG GAGGAATTTT GCAGATGTAT TATGAACAGG GATTAGAAAA ACCATTTAAA 
GAGATACAGC TTGATCCATA TTGTAGGCTT TCTGAACCCA AGGTTGAGAA CTTCAGTGTA 
GCAGGAAAAA TCCACACTGT GAAGATTGAA CATGTGTCTT ACACAGAAAA AAGGAAATAC 
CATTCTAAGA CAGAAGTAGT TCATGAACCT GACATAGAGC AGATGCTGAA GTTGGGGTCC 
ACATCGTACC ATGACTTCCT TGACTTTCTG ACTACTGTGG AGGAGGAGCT GATGAAGTTG 
CCAGCTGTTT CAAAACCAAA AAAGAACTAC GAGGAGCAAG AAATTTCCTT GGAAATTGTG 
GACAACTTTT GGGGTAAAGT CACAAAAGAA GGAAAATTTG TTGAAAGTGC TGTGATAACT 
CAAATTTATT GCCTCTGCTT TGTGAATGGG AACCTGGAAT GCTTTTTAAC CTTGAATGAC 
CTTGAGTTGC CGAAGCGAGA TGAATCCTAT TATGAGAAGG ACTCAGAAAA AAAGGGGATT 
GATATTCTTG ACTACCATTT TCATAAGTGT GTGAATGTAC AAGAATTTGA GCAATCAAGA 
ATCATTAAGT TTGTACCTCT GGATGCCTGC CGGTTTGAGC TGATGCGTTT CAAGACTTTG 
TATAATGGGG ATAATCTTCC CTTTTCCTTG AAGTCTGTAG TGGTTGTCCA GGGAGCATAC 
GTGGAACTTC AGGCTTTTGT CAACATGGCC TCATTGGCGC AGAGGTCATC CTATGCTGGT 
TCCTTAAGGT CCTGTGACAA TATAAGGATA CACTTTCCTG TCCCATCGCA GTGGATCAAG 
GCCCTTTGGA CCATGAACCT CCAGAGGCAG AAGTCTCTGA AAGCTAAAAT GAACCGCCGA 
GCATGTCTGG GGAGTTTACA GGAACTTGAA TCTGAACCTG TCATTCAAGT CACTGTGGGG 
TCAGCAAAAT ATGAGAGTGC CTACCAGGCA GTGGTATGGA AGATAGATCG GCTTCCAGAC 
AAAAATTCAA GTCTAGATCA TCCCCATTGT CTGTCATACA AATTAGAGCT TGGATCAGAC 
CAAGAAATTC CCTCTGATTG GTATCCATTT GCTACTGTTC AGTTTTCCGT GCCTGACACC 
TGTGCCTCAA GGACAGAGGT CAGGTCTCTG GGAGTGGAGA GTGATGTCCA GCCACAGAAA 
CATGTTCAGC AGCGAGCTTG CTACAACATC CAGGTTGAAA TAGAAAAGAA GTGGATTAAA 
ATCGATGGAG AAGACCCAGA TAAAATTGGT GACTGCATAA CTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

STON1 ( NM_001198595.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001185524.1 Homo sapiens stonin 1 (STON1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu10282
Clone ID Related Accession (Same CDS sequence)	NM_006873.3 , NM_001198595.1
Accession Version	NM_001198595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-01
Organism	Homo sapiens(human)
Product	stonin-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB288942.1, AC073082.6, AF255310.1 and BM974074.1. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: CR936807.1, SRR1803617.91296.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGCTCCA CAAATCCAGG CAAATGGGTC ACCTTTGATG ATGATCCTGC TGTTCAATCT 
TCTCAAAAGT CAAAGAATTT TCCTCTGGAG AATCAAGGTG TCTGTAGACC AAATGGACTG 
AAGCTGAACC TTCCTGGCCT CAGGGAATTT CCCAGTGGAT CTTCCTCCAC CAGCAGCACT 
CCTCTCTCCT CCCCCATTGT AGATTTTTAT TTCAGTCCAG GACCTCCAAG TAACTCTCCT 
CTTTCTACAC CTACCAAAGA CTTCCCAGGT TTTCCTGGCA TCCCCAAAGC AGGGACTCAT 
GTGCTTTATC CTATTCCAGA ATCATCTTCA GACAGCCCAC TCGCAATATC AGGAGGAGAA 
TCTTCCTTAC TGCCTACCAG ACCAACATGT TTATCCCATG CCTTGTTACC CAGTGACCAC 
TCATGTACAC ATCCAACTCC CAAAGTAGGT CTTCCAGATG AAGTTAATCC TCAACAGGCT 
GAAAGCCTAG GATTCCAAAG TGATGATCTC CCCCAGTTTC AGTATTTTCG AGAGGACTGT 
GCTTTTTCAA GTCCATTTTG GAAAGATGAA GGCAGTGATT CCCATTTCAC CCTTGACCCA 
CCAGGAAGCA AAAAGATGTT CTCATCAAGA AACAAGGAGA TGCCTATTGA CCAAAAAAGC 
CTAAATAAGT GTTCACTCAA CTATATCTGT GAGAAGCTTG AACATCTCCA GTCAGCTGAG 
AACCAAGACT CACTTAGAAG TTTGTCTATG CACTGTCTAT GTGCTGAAGA AAATGCCTCT 
TCCTTTGTCC CCCACACACT CTTCAGGAGT CAGCCAAAAT CCGGATGGTC TTTCATGCTG 
AGAATTCCTG AGAAGAAGAA TATGATGTCT TCCCGGCAAT GGGGACCAAT TTTTCTGAAA 
GTTTTGCCTG GAGGAATTTT GCAGATGTAT TATGAACAGG GATTAGAAAA ACCATTTAAA 
GAGATACAGC TTGATCCATA TTGTAGGCTT TCTGAACCCA AGGTTGAGAA CTTCAGTGTA 
GCAGGAAAAA TCCACACTGT GAAGATTGAA CATGTGTCTT ACACAGAAAA AAGGAAATAC 
CATTCTAAGA CAGAAGTAGT TCATGAACCT GACATAGAGC AGATGCTGAA GTTGGGGTCC 
ACATCGTACC ATGACTTCCT TGACTTTCTG ACTACTGTGG AGGAGGAGCT GATGAAGTTG 
CCAGCTGTTT CAAAACCAAA AAAGAACTAC GAGGAGCAAG AAATTTCCTT GGAAATTGTG 
GACAACTTTT GGGGTAAAGT CACAAAAGAA GGAAAATTTG TTGAAAGTGC TGTGATAACT 
CAAATTTATT GCCTCTGCTT TGTGAATGGG AACCTGGAAT GCTTTTTAAC CTTGAATGAC 
CTTGAGTTGC CGAAGCGAGA TGAATCCTAT TATGAGAAGG ACTCAGAAAA AAAGGGGATT 
GATATTCTTG ACTACCATTT TCATAAGTGT GTGAATGTAC AAGAATTTGA GCAATCAAGA 
ATCATTAAGT TTGTACCTCT GGATGCCTGC CGGTTTGAGC TGATGCGTTT CAAGACTTTG 
TATAATGGGG ATAATCTTCC CTTTTCCTTG AAGTCTGTAG TGGTTGTCCA GGGAGCATAC 
GTGGAACTTC AGGCTTTTGT CAACATGGCC TCATTGGCGC AGAGGTCATC CTATGCTGGT 
TCCTTAAGGT CCTGTGACAA TATAAGGATA CACTTTCCTG TCCCATCGCA GTGGATCAAG 
GCCCTTTGGA CCATGAACCT CCAGAGGCAG AAGTCTCTGA AAGCTAAAAT GAACCGCCGA 
GCATGTCTGG GGAGTTTACA GGAACTTGAA TCTGAACCTG TCATTCAAGT CACTGTGGGG 
TCAGCAAAAT ATGAGAGTGC CTACCAGGCA GTGGTATGGA AGATAGATCG GCTTCCAGAC 
AAAAATTCAA GTCTAGATCA TCCCCATTGT CTGTCATACA AATTAGAGCT TGGATCAGAC 
CAAGAAATTC CCTCTGATTG GTATCCATTT GCTACTGTTC AGTTTTCCGT GCCTGACACC 
TGTGCCTCAA GGACAGAGGT CAGGTCTCTG GGAGTGGAGA GTGATGTCCA GCCACAGAAA 
CATGTTCAGC AGCGAGCTTG CTACAACATC CAGGTTGAAA TAGAAAAGAA GTGGATTAAA 
ATCGATGGAG AAGACCCAGA TAAAATTGGT GACTGCATAA CTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185524.1
CDS	213..2420
Translation	MCSTNPGKWVTFDDDPAVQSSQKSKNFPLENQGVCRPNGLKLNLPGLREFPSGSSSTSSTPLSSPIVDFYFSPGPPSNSPLSTPTKDFPGFPGIPKAGTHVLYPIPESSSDSPLAISGGESSLLPTRPTCLSHALLPSDHSCTHPTPKVGLPDEVNPQQAESLGFQSDDLPQFQYFREDCAFSSPFWKDEGSDSHFTLDPPGSKKMFSSRNKEMPIDQKSLNKCSLNYICEKLEHLQSAENQDSLRSLSMHCLCAEENASSFVPHTLFRSQPKSGWSFMLRIPEKKNMMSSRQWGPIFLKVLPGGILQMYYEQGLEKPFKEIQLDPYCRLSEPKVENFSVAGKIHTVKIEHVSYTEKRKYHSKTEVVHEPDIEQMLKLGSTSYHDFLDFLTTVEEELMKLPAVSKPKKNYEEQEISLEIVDNFWGKVTKEGKFVESAVITQIYCLCFVNGNLECFLTLNDLELPKRDESYYEKDSEKKGIDILDYHFHKCVNVQEFEQSRIIKFVPLDACRFELMRFKTLYNGDNLPFSLKSVVVVQGAYVELQAFVNMASLAQRSSYAGSLRSCDNIRIHFPVPSQWIKALWTMNLQRQKSLKAKMNRRACLGSLQELESEPVIQVTVGSAKYESAYQAVVWKIDRLPDKNSSLDHPHCLSYKLELGSDQEIPSDWYPFATVQFSVPDTCASRTEVRSLGVESDVQPQKHVQQRACYNIQVEIEKKWIKIDGEDPDKIGDCITQ

Target ORF information:

RefSeq Version	NM_001198595.1
Organism	Homo sapiens(human)
Definition	Homo sapiens stonin 1 (STON1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198595.1

ORF Insert Sequence:

ATGTGCTCCA CAAATCCAGG CAAATGGGTC ACCTTTGATG ATGATCCTGC TGTTCAATCT 
TCTCAAAAGT CAAAGAATTT TCCTCTGGAG AATCAAGGTG TCTGTAGACC AAATGGACTG 
AAGCTGAACC TTCCTGGCCT CAGGGAATTT CCCAGTGGAT CTTCCTCCAC CAGCAGCACT 
CCTCTCTCCT CCCCCATTGT AGATTTTTAT TTCAGTCCAG GACCTCCAAG TAACTCTCCT 
CTTTCTACAC CTACCAAAGA CTTCCCAGGT TTTCCTGGCA TCCCCAAAGC AGGGACTCAT 
GTGCTTTATC CTATTCCAGA ATCATCTTCA GACAGCCCAC TCGCAATATC AGGAGGAGAA 
TCTTCCTTAC TGCCTACCAG ACCAACATGT TTATCCCATG CCTTGTTACC CAGTGACCAC 
TCATGTACAC ATCCAACTCC CAAAGTAGGT CTTCCAGATG AAGTTAATCC TCAACAGGCT 
GAAAGCCTAG GATTCCAAAG TGATGATCTC CCCCAGTTTC AGTATTTTCG AGAGGACTGT 
GCTTTTTCAA GTCCATTTTG GAAAGATGAA GGCAGTGATT CCCATTTCAC CCTTGACCCA 
CCAGGAAGCA AAAAGATGTT CTCATCAAGA AACAAGGAGA TGCCTATTGA CCAAAAAAGC 
CTAAATAAGT GTTCACTCAA CTATATCTGT GAGAAGCTTG AACATCTCCA GTCAGCTGAG 
AACCAAGACT CACTTAGAAG TTTGTCTATG CACTGTCTAT GTGCTGAAGA AAATGCCTCT 
TCCTTTGTCC CCCACACACT CTTCAGGAGT CAGCCAAAAT CCGGATGGTC TTTCATGCTG 
AGAATTCCTG AGAAGAAGAA TATGATGTCT TCCCGGCAAT GGGGACCAAT TTTTCTGAAA 
GTTTTGCCTG GAGGAATTTT GCAGATGTAT TATGAACAGG GATTAGAAAA ACCATTTAAA 
GAGATACAGC TTGATCCATA TTGTAGGCTT TCTGAACCCA AGGTTGAGAA CTTCAGTGTA 
GCAGGAAAAA TCCACACTGT GAAGATTGAA CATGTGTCTT ACACAGAAAA AAGGAAATAC 
CATTCTAAGA CAGAAGTAGT TCATGAACCT GACATAGAGC AGATGCTGAA GTTGGGGTCC 
ACATCGTACC ATGACTTCCT TGACTTTCTG ACTACTGTGG AGGAGGAGCT GATGAAGTTG 
CCAGCTGTTT CAAAACCAAA AAAGAACTAC GAGGAGCAAG AAATTTCCTT GGAAATTGTG 
GACAACTTTT GGGGTAAAGT CACAAAAGAA GGAAAATTTG TTGAAAGTGC TGTGATAACT 
CAAATTTATT GCCTCTGCTT TGTGAATGGG AACCTGGAAT GCTTTTTAAC CTTGAATGAC 
CTTGAGTTGC CGAAGCGAGA TGAATCCTAT TATGAGAAGG ACTCAGAAAA AAAGGGGATT 
GATATTCTTG ACTACCATTT TCATAAGTGT GTGAATGTAC AAGAATTTGA GCAATCAAGA 
ATCATTAAGT TTGTACCTCT GGATGCCTGC CGGTTTGAGC TGATGCGTTT CAAGACTTTG 
TATAATGGGG ATAATCTTCC CTTTTCCTTG AAGTCTGTAG TGGTTGTCCA GGGAGCATAC 
GTGGAACTTC AGGCTTTTGT CAACATGGCC TCATTGGCGC AGAGGTCATC CTATGCTGGT 
TCCTTAAGGT CCTGTGACAA TATAAGGATA CACTTTCCTG TCCCATCGCA GTGGATCAAG 
GCCCTTTGGA CCATGAACCT CCAGAGGCAG AAGTCTCTGA AAGCTAAAAT GAACCGCCGA 
GCATGTCTGG GGAGTTTACA GGAACTTGAA TCTGAACCTG TCATTCAAGT CACTGTGGGG 
TCAGCAAAAT ATGAGAGTGC CTACCAGGCA GTGGTATGGA AGATAGATCG GCTTCCAGAC 
AAAAATTCAA GTCTAGATCA TCCCCATTGT CTGTCATACA AATTAGAGCT TGGATCAGAC 
CAAGAAATTC CCTCTGATTG GTATCCATTT GCTACTGTTC AGTTTTCCGT GCCTGACACC 
TGTGCCTCAA GGACAGAGGT CAGGTCTCTG GGAGTGGAGA GTGATGTCCA GCCACAGAAA 
CATGTTCAGC AGCGAGCTTG CTACAACATC CAGGTTGAAA TAGAAAAGAA GTGGATTAAA 
ATCGATGGAG AAGACCCAGA TAAAATTGGT GACTGCATAA CTCAGTAG