crim1 cDNA ORF clone,

Gene
Clones
gRNAs

The following crim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBl71238	XM_028438330.1 Latest version!	Gouania willdenowi cysteine rich transmembrane BMP regulator 1 (crim1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBl71239	XM_028438331.1 Latest version!	Gouania willdenowi cysteine rich transmembrane BMP regulator 1 (crim1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crim1 ( XM_028438330.1 ) cDNA ORF clone, -> XP_028294131.1 Gouania willdenowi cysteine rich transmembrane BMP regulator 1 (crim1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBl71238
Clone ID Related Accession (Same CDS sequence)	XM_028438330.1
Accession Version	XM_028438330.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-17
Organism	Gouania willdenowi(blunt-snouted clingfish)
Product	cysteine-rich motor neuron 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041065.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gouania willdenowi Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGTTC TCCTGTTGAA TGTCATCCTA ACGGTGCGGG CGCTCACAGC GCACGAGTGC 
ACGCCGTGTG ACCTCCGGAC ATGTCCAGTC AGATCCCCGC ACACATCATG TGACCATCCT 
CGGACCCTGG CCAGGGATCC GTGCGGATGC TGCGATCAGT GCGCCCGGCT GGAGTGGGAG 
CCCTGCGGGG GCCAGGATTG GGTGCTCGGG TACTGCGCCC TGGGTTTGAC GTGTGCATCT 
GTCAACAAAA CCGGAGCTGC CAGCGTCCCC GAAATTGGGG TGTGCAAAGT ACTTCCGGAT 
AATCCAGAGG CAGGTCTTGT AGATGAGCGC TGTCCGCTGG TGACAGGCTG CGACAGAGCC 
GGAGGTCAGT GTGTGTGTGA TGCCCGCCAC ACCTGCTTGG ATTCCTTCAC CTATGCAGAC 
CAAGAAACCT GCATGAAAGT CACCAAGTCA GATGGGAAAG CATCCGAGCA CCGGGAGAAG 
CACAGAGAGA AGTATGTGGG ACCCTCCAAC CCGGCCTGCA TGTTCTCGGG CTGTAACCTG 
ACTGCTGAGG GCTGTGTGTG CGAGTCCAAA AGCTGCCAGC ATCACTTCAC CTACATTAAC 
CGCAGCCAGT GCCAAGAAGT TGCAGATGAG CTGAGGTGTG CCAGGGTGAT GTGCCCTCCT 
CTGAAGGTGC CATCTTGCCC CAAGGGTTCA TTCCTGACAA AAAGCTACAC GCCCCCTGCT 
GGTTGCTGCC CCTCCATACT ACCACAGTGT ACTTGCGATC TCAGTGCCTG CCACAAACCC 
CACTGTCCAC GTGGACAGCA GCCCATTCCA CTAAATCAGG CCAGTGGGCA GCCGGGAGAC 
TGCTGTGATG TCTTTGAGTG CCAGAAAGTT TCTCCCAAGT GTATCTACAA TGAAAAGGAG 
TTTGCAGAGG GAGAGGTGTA CCGTATGGAT TCTTGCTGGT TGTGTCAGTG CCGTGGAGGA 
ATATCCTTCT GCTCCAAGGC AGAGTGTGCC GAACTGGATT GTGAAAACTT CTACATTCCA 
GAGGGCGAGT GTTGCCCTGT CTGCATAGAT GTAGAGTTGC TATCAATGGA CAGCACTAAG 
GCCAGCTGCT GGGTTGACAA CAAGCTGCGA GCCCACGAGG AGCAGTGGAA GGAGGACGAC 
TGCACCTTCT GCCAGTGTGT GGATGGAGAC CCTCACTGCA CGGCCATGGC CTGCAAACAG 
AGCTGCCAGA ACCCTGTGAA GATCCCCGGG GAGTGTTGTC CCTTCTGTGA AGAGCCTTCA 
TATGAAACCG TTAGCCCTTT GCTCTGTCCT GCAATGGAAA ACTGCTCCCT GTCAACCAAC 
GACTGCCCCT ATGGCTTCCT CCAAGACAAG AATGGATGTC TGCTATGCCA GTGTCTTAAT 
AATGATTCCT GTCCTGACCT GGAGAGATAC TGCTCACTGC AATGCCCAGT GGGATATGAA 
CGAGATGATT TTGGGTGCGA AGTGTGTGAA TGTAGTATAC CCACACCACG GTGTCGACCT 
TTGATCTGCA CCAAGACCTG CCCCTATGGA TATGTGAGGA ACAAGCACGG CTGTGAAATG 
TGTCGCTGCG TCAAGTGTCC TCCTTTCACC TGCGATAAGC ACTGCAGCAA CGGCTATCAA 
CAGAACAGAA AGGGCTGCTC CACCTGCATG TGTAAAGAAA GTCCTCATAT ACCAAGGACC 
ACTGTTCCAG CCCCGGTGCC AAGCTATTGC CTCACCTCCA ATGGGCACCG ATACGAGGAG 
GGCGACGGCT GGCATGACGG CTGTCGGGAT TGCTACTGTC ACTCTGGAAG GGAGATGTGT 
GTCCTGATAT CCTGCCCTGT CCCCAGCTGC TCTAATCCTA TAGTCAAACC AGACCAGTGT 
TGTCCTACCT GTGAAGATGA GTCGGGCAGC GGTCAGCCAG AGGGAATGGA CATGGTGGTG 
TGCAGAGCAC CTGGGGGAGA GTTCTATGTG GAAGGGGAGA CCTGGAACCT GGACGAGTGC 
ACACGGTGCA CCTGCAGAAA GGGGCGTGTC TTGTGCGACA CTGAAGTGTG TCCTCCGGCT 
CTCTGCCAGA CACCAATCAG GAACAAGGAC ACGTGTTGCC ATGTGTGCCC AGAGGAGACG 
TTGAGCCCCT TGCTCCCAGT AAACAGCAGC CAGCAGGAGT ACTGCATTAC CAGCGATGGA 
GACGTACTGT TGACTGGAGA CTCCTGGAGG GCCAATGCCT GCACAAGCTG CACCTGCAAC 
AACGGCACCA TTCAGTGTTT TTCCCAGCAC TGCCCCGCCA CCAACTGCCG GGTGCCCGTT 
TTACGCAAGG GCCAATGCTG CCCACACTGT CTCGAGGTGA CAACATCAGT GCCCGTGAGA 
TTTTCTACGA GTCGACCCAA AGCAAGAGTC ACTACTACTA CAATGACAAA TCCTGCATTC 
ATCACTACTG TTACAATGCC AAACATCGTT GCTGAAACAG GTGAACCAAG TTTTGGTGAG 
AATTACCCAA GCCAAACGAA GATGACCCTG ATCTACCAGT CTGCAGCTTG GATCCTGGCT 
GGACTTCTTT TAGCCATCAT TATCTTCCTC ATCGTGGCGT TGCTAATCAA CAAGAAGAAG 
AAGTGGGTGC AGATGTCGTG CTACAGCGCG CCAAAGAAGA CCGTGATTCT CAAAAAGCAC 
CTAAACAAGA ATTCGGTGGT CTACATGGAG CCCTCCAAGG AGAATAAATT TCAAAATGTG 
AACGACTGTG GGATCATCCA CTTCTCTCCA GATATGAGCT CCACCTGCAG GGAGAAGATG 
ACCATCCCCA GAGCCAAGCT GGGCATAGCA AACGACTGGA AACCTCGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028294131.1
CDS	327..3137
Translation	MLVLLLNVILTVRALTAHECTPCDLRTCPVRSPHTSCDHPRTLARDPCGCCDQCARLEWEPCGGQDWVLGYCALGLTCASVNKTGAASVPEIGVCKVLPDNPEAGLVDERCPLVTGCDRAGGQCVCDARHTCLDSFTYADQETCMKVTKSDGKASEHREKHREKYVGPSNPACMFSGCNLTAEGCVCESKSCQHHFTYINRSQCQEVADELRCARVMCPPLKVPSCPKGSFLTKSYTPPAGCCPSILPQCTCDLSACHKPHCPRGQQPIPLNQASGQPGDCCDVFECQKVSPKCIYNEKEFAEGEVYRMDSCWLCQCRGGISFCSKAECAELDCENFYIPEGECCPVCIDVELLSMDSTKASCWVDNKLRAHEEQWKEDDCTFCQCVDGDPHCTAMACKQSCQNPVKIPGECCPFCEEPSYETVSPLLCPAMENCSLSTNDCPYGFLQDKNGCLLCQCLNNDSCPDLERYCSLQCPVGYERDDFGCEVCECSIPTPRCRPLICTKTCPYGYVRNKHGCEMCRCVKCPPFTCDKHCSNGYQQNRKGCSTCMCKESPHIPRTTVPAPVPSYCLTSNGHRYEEGDGWHDGCRDCYCHSGREMCVLISCPVPSCSNPIVKPDQCCPTCEDESGSGQPEGMDMVVCRAPGGEFYVEGETWNLDECTRCTCRKGRVLCDTEVCPPALCQTPIRNKDTCCHVCPEETLSPLLPVNSSQQEYCITSDGDVLLTGDSWRANACTSCTCNNGTIQCFSQHCPATNCRVPVLRKGQCCPHCLEVTTSVPVRFSTSRPKARVTTTTMTNPAFITTVTMPNIVAETGEPSFGENYPSQTKMTLIYQSAAWILAGLLLAIIIFLIVALLINKKKKWVQMSCYSAPKKTVILKKHLNKNSVVYMEPSKENKFQNVNDCGIIHFSPDMSSTCREKMTIPRAKLGIANDWKPR

Target ORF information:

RefSeq Version	XM_028438330.1
Organism	Gouania willdenowi(blunt-snouted clingfish)
Definition	Gouania willdenowi cysteine rich transmembrane BMP regulator 1 (crim1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028438330.1

ORF Insert Sequence:

ATGCTCGTTC TCCTGTTGAA TGTCATCCTA ACGGTGCGGG CGCTCACAGC GCACGAGTGC 
ACGCCGTGTG ACCTCCGGAC ATGTCCAGTC AGATCCCCGC ACACATCATG TGACCATCCT 
CGGACCCTGG CCAGGGATCC GTGCGGATGC TGCGATCAGT GCGCCCGGCT GGAGTGGGAG 
CCCTGCGGGG GCCAGGATTG GGTGCTCGGG TACTGCGCCC TGGGTTTGAC GTGTGCATCT 
GTCAACAAAA CCGGAGCTGC CAGCGTCCCC GAAATTGGGG TGTGCAAAGT ACTTCCGGAT 
AATCCAGAGG CAGGTCTTGT AGATGAGCGC TGTCCGCTGG TGACAGGCTG CGACAGAGCC 
GGAGGTCAGT GTGTGTGTGA TGCCCGCCAC ACCTGCTTGG ATTCCTTCAC CTATGCAGAC 
CAAGAAACCT GCATGAAAGT CACCAAGTCA GATGGGAAAG CATCCGAGCA CCGGGAGAAG 
CACAGAGAGA AGTATGTGGG ACCCTCCAAC CCGGCCTGCA TGTTCTCGGG CTGTAACCTG 
ACTGCTGAGG GCTGTGTGTG CGAGTCCAAA AGCTGCCAGC ATCACTTCAC CTACATTAAC 
CGCAGCCAGT GCCAAGAAGT TGCAGATGAG CTGAGGTGTG CCAGGGTGAT GTGCCCTCCT 
CTGAAGGTGC CATCTTGCCC CAAGGGTTCA TTCCTGACAA AAAGCTACAC GCCCCCTGCT 
GGTTGCTGCC CCTCCATACT ACCACAGTGT ACTTGCGATC TCAGTGCCTG CCACAAACCC 
CACTGTCCAC GTGGACAGCA GCCCATTCCA CTAAATCAGG CCAGTGGGCA GCCGGGAGAC 
TGCTGTGATG TCTTTGAGTG CCAGAAAGTT TCTCCCAAGT GTATCTACAA TGAAAAGGAG 
TTTGCAGAGG GAGAGGTGTA CCGTATGGAT TCTTGCTGGT TGTGTCAGTG CCGTGGAGGA 
ATATCCTTCT GCTCCAAGGC AGAGTGTGCC GAACTGGATT GTGAAAACTT CTACATTCCA 
GAGGGCGAGT GTTGCCCTGT CTGCATAGAT GTAGAGTTGC TATCAATGGA CAGCACTAAG 
GCCAGCTGCT GGGTTGACAA CAAGCTGCGA GCCCACGAGG AGCAGTGGAA GGAGGACGAC 
TGCACCTTCT GCCAGTGTGT GGATGGAGAC CCTCACTGCA CGGCCATGGC CTGCAAACAG 
AGCTGCCAGA ACCCTGTGAA GATCCCCGGG GAGTGTTGTC CCTTCTGTGA AGAGCCTTCA 
TATGAAACCG TTAGCCCTTT GCTCTGTCCT GCAATGGAAA ACTGCTCCCT GTCAACCAAC 
GACTGCCCCT ATGGCTTCCT CCAAGACAAG AATGGATGTC TGCTATGCCA GTGTCTTAAT 
AATGATTCCT GTCCTGACCT GGAGAGATAC TGCTCACTGC AATGCCCAGT GGGATATGAA 
CGAGATGATT TTGGGTGCGA AGTGTGTGAA TGTAGTATAC CCACACCACG GTGTCGACCT 
TTGATCTGCA CCAAGACCTG CCCCTATGGA TATGTGAGGA ACAAGCACGG CTGTGAAATG 
TGTCGCTGCG TCAAGTGTCC TCCTTTCACC TGCGATAAGC ACTGCAGCAA CGGCTATCAA 
CAGAACAGAA AGGGCTGCTC CACCTGCATG TGTAAAGAAA GTCCTCATAT ACCAAGGACC 
ACTGTTCCAG CCCCGGTGCC AAGCTATTGC CTCACCTCCA ATGGGCACCG ATACGAGGAG 
GGCGACGGCT GGCATGACGG CTGTCGGGAT TGCTACTGTC ACTCTGGAAG GGAGATGTGT 
GTCCTGATAT CCTGCCCTGT CCCCAGCTGC TCTAATCCTA TAGTCAAACC AGACCAGTGT 
TGTCCTACCT GTGAAGATGA GTCGGGCAGC GGTCAGCCAG AGGGAATGGA CATGGTGGTG 
TGCAGAGCAC CTGGGGGAGA GTTCTATGTG GAAGGGGAGA CCTGGAACCT GGACGAGTGC 
ACACGGTGCA CCTGCAGAAA GGGGCGTGTC TTGTGCGACA CTGAAGTGTG TCCTCCGGCT 
CTCTGCCAGA CACCAATCAG GAACAAGGAC ACGTGTTGCC ATGTGTGCCC AGAGGAGACG 
TTGAGCCCCT TGCTCCCAGT AAACAGCAGC CAGCAGGAGT ACTGCATTAC CAGCGATGGA 
GACGTACTGT TGACTGGAGA CTCCTGGAGG GCCAATGCCT GCACAAGCTG CACCTGCAAC 
AACGGCACCA TTCAGTGTTT TTCCCAGCAC TGCCCCGCCA CCAACTGCCG GGTGCCCGTT 
TTACGCAAGG GCCAATGCTG CCCACACTGT CTCGAGGTGA CAACATCAGT GCCCGTGAGA 
TTTTCTACGA GTCGACCCAA AGCAAGAGTC ACTACTACTA CAATGACAAA TCCTGCATTC 
ATCACTACTG TTACAATGCC AAACATCGTT GCTGAAACAG GTGAACCAAG TTTTGGTGAG 
AATTACCCAA GCCAAACGAA GATGACCCTG ATCTACCAGT CTGCAGCTTG GATCCTGGCT 
GGACTTCTTT TAGCCATCAT TATCTTCCTC ATCGTGGCGT TGCTAATCAA CAAGAAGAAG 
AAGTGGGTGC AGATGTCGTG CTACAGCGCG CCAAAGAAGA CCGTGATTCT CAAAAAGCAC 
CTAAACAAGA ATTCGGTGGT CTACATGGAG CCCTCCAAGG AGAATAAATT TCAAAATGTG 
AACGACTGTG GGATCATCCA CTTCTCTCCA GATATGAGCT CCACCTGCAG GGAGAAGATG 
ACCATCCCCA GAGCCAAGCT GGGCATAGCA AACGACTGGA AACCTCGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crim1 ( XM_028438331.1 ) cDNA ORF clone, -> XP_028294132.1 Gouania willdenowi cysteine rich transmembrane BMP regulator 1 (crim1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBl71239
Clone ID Related Accession (Same CDS sequence)	XM_028438331.1
Accession Version	XM_028438331.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-17
Organism	Gouania willdenowi(blunt-snouted clingfish)
Product	cysteine-rich motor neuron 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041065.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gouania willdenowi Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGTTC TCCTGTTGAA TGTCATCCTA ACGGTGCGGG CGCTCACAGC GCACGAGTGC 
ACGCCGTGTG ACCTCCGGAC ATGTCCAGTC AGATCCCCGC ACACATCATG TGACCATCCT 
CGGACCCTGG CCAGGGATCC GTGCGGATGC TGCGATCAGT GCGCCCGGCT GGAGTGGGAG 
CCCTGCGGGG GCCAGGATTG GGTGCTCGGG TACTGCGCCC TGGGTTTGAC GTGTGCATCT 
GTCAACAAAA CCGGAGCTGC CAGCGTCCCC GAAATTGGGG TGTGCAAAGT ACTTCCGGAT 
AATCCAGAGG CAGGTCTTGT AGATGAGCGC TGTCCGCTGG TGACAGGCTG CGACAGAGCC 
GGAGGTCAGT GTGTGTGTGA TGCCCGCCAC ACCTGCTTGG ATTCCTTCAC CTATGCAGAC 
CAAGAAACCT GCATGAAAGT CACCAAGTCA GATGGGAAAG CATCCGAGCA CCGGGAGAAG 
CACAGAGAGA AGTATGTGGG ACCCTCCAAC CCGGCCTGCA TGTTCTCGGG CTGTAACCTG 
ACTGCTGAGG GCTGTGTGTG CGAGTCCAAA AGCTGCCAGC ATCACTTCAC CTACATTAAC 
CGCAGCCAGT GCCAAGAAGT TGCAGATGAG CTGAGGTGTG CCAGGGTGAT GTGCCCTCCT 
CTGAAGGTGC CATCTTGCCC CAAGGGTTCA TTCCTGACAA AAAGCTACAC GCCCCCTGCT 
GGTTGCTGCC CCTCCATACT ACCACAGTGT ACTTGCGATC TCAGTGCCTG CCACAAACCC 
CACTGTCCAC GTGGACAGCA GCCCATTCCA CTAAATCAGG CCAGTGGGCA GCCGGGAGAC 
TGCTGTGATG TCTTTGAGTG CCAGAAAGTT TCTCCCAAGT GTATCTACAA TGAAAAGGAG 
TTTGCAGAGG GAGAGGTGTA CCGTATGGAT TCTTGCTGGT TGTGTCAGTG CCGTGGAGGA 
ATATCCTTCT GCTCCAAGGC AGAGTGTGCC GAACTGGATT GTGAAAACTT CTACATTCCA 
GAGGGCGAGT GTTGCCCTGT CTGCATAGAT GTAGAGTTGC TATCAATGGA CAGCACTAAG 
GCCAGCTGCT GGGTTGACAA CAAGCTGCGA GCCCACGAGG AGCAGTGGAA GGAGGACGAC 
TGCACCTTCT GCCAGTGTGT GGATGGAGAC CCTCACTGCA CGGCCATGGC CTGCAAACAG 
AGCTGCCAGA ACCCTGTGAA GATCCCCGGG GAGTGTTGTC CCTTCTGTGA AGAGCCTTCA 
TATGAAACCG TTAGCCCTTT GCTCTGTCCT GCAATGGAAA ACTGCTCCCT GTCAACCAAC 
GACTGCCCCT ATGGCTTCCT CCAAGACAAG AATGGATGTC TGCTATGCCA GTGTCTTAAT 
AATGATTCCT GTCCTGACCT GGAGAGATAC TGCTCACTGC AATGCCCAGT GGGATATGAA 
CGAGATGATT TTGGGTGCGA AGTGTGTGAA TGTAGTATAC CCACACCACG GTGTCGACCT 
TTGATCTGCA CCAAGACCTG CCCCTATGGA TATGTGAGGA ACAAGCACGG CTGTGAAATG 
TGTCGCTGCG TCAAGTGTCC TCCTTTCACC TGCGATAAGC ACTGCAGCAA CGGCTATCAA 
CAGAACAGAA AGGGCTGCTC CACCTGCATG TGTAAAGAAA GTCCTCATAT ACCAAGGACC 
ACTGTTCCAG CCCCGGTGCC AAGCTATTGC CTCACCTCCA ATGGGCACCG ATACGAGGAG 
GGCGACGGCT GGCATGACGG CTGTCGGGAT TGCTACTGTC ACTCTGGAAG GGAGATGTGT 
GTCCTGATAT CCTGCCCTGT CCCCAGCTGC TCTAATCCTA TAGTCAAACC AGACCAGTGT 
TGTCCTACCT GTGAAGATGA GTCGGGCAGC GGTCAGCCAG AGGGAATGGA CATGGTGGTG 
TGCAGAGCAC CTGGGGGAGA GTTCTATGTG GAAGGGGAGA CCTGGAACCT GGACGAGTGC 
ACACGGTGCA CCTGCAGAAA GGGGCGTGTC TTGTGCGACA CTGAAGTGTG TCCTCCGGCT 
CTCTGCCAGA CACCAATCAG GAACAAGGAC ACGTGTTGCC ATGTGTGCCC AGAGGTGACA 
ACATCAGTGC CCGTGAGATT TTCTACGAGT CGACCCAAAG CAAGAGTCAC TACTACTACA 
ATGACAAATC CTGCATTCAT CACTACTGTT ACAATGCCAA ACATCGTTGC TGAAACAGGT 
GAACCAAGTT TTGGTGAGAA TTACCCAAGC CAAACGAAGA TGACCCTGAT CTACCAGTCT 
GCAGCTTGGA TCCTGGCTGG ACTTCTTTTA GCCATCATTA TCTTCCTCAT CGTGGCGTTG 
CTAATCAACA AGAAGAAGAA GTGGGTGCAG ATGTCGTGCT ACAGCGCGCC AAAGAAGACC 
GTGATTCTCA AAAAGCACCT AAACAAGAAT TCGGTGGTCT ACATGGAGCC CTCCAAGGAG 
AATAAATTTC AAAATGTGAA CGACTGTGGG ATCATCCACT TCTCTCCAGA TATGAGCTCC 
ACCTGCAGGG AGAAGATGAC CATCCCCAGA GCCAAGCTGG GCATAGCAAA CGACTGGAAA 
CCTCGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028294132.1
CDS	327..2915
Translation	MLVLLLNVILTVRALTAHECTPCDLRTCPVRSPHTSCDHPRTLARDPCGCCDQCARLEWEPCGGQDWVLGYCALGLTCASVNKTGAASVPEIGVCKVLPDNPEAGLVDERCPLVTGCDRAGGQCVCDARHTCLDSFTYADQETCMKVTKSDGKASEHREKHREKYVGPSNPACMFSGCNLTAEGCVCESKSCQHHFTYINRSQCQEVADELRCARVMCPPLKVPSCPKGSFLTKSYTPPAGCCPSILPQCTCDLSACHKPHCPRGQQPIPLNQASGQPGDCCDVFECQKVSPKCIYNEKEFAEGEVYRMDSCWLCQCRGGISFCSKAECAELDCENFYIPEGECCPVCIDVELLSMDSTKASCWVDNKLRAHEEQWKEDDCTFCQCVDGDPHCTAMACKQSCQNPVKIPGECCPFCEEPSYETVSPLLCPAMENCSLSTNDCPYGFLQDKNGCLLCQCLNNDSCPDLERYCSLQCPVGYERDDFGCEVCECSIPTPRCRPLICTKTCPYGYVRNKHGCEMCRCVKCPPFTCDKHCSNGYQQNRKGCSTCMCKESPHIPRTTVPAPVPSYCLTSNGHRYEEGDGWHDGCRDCYCHSGREMCVLISCPVPSCSNPIVKPDQCCPTCEDESGSGQPEGMDMVVCRAPGGEFYVEGETWNLDECTRCTCRKGRVLCDTEVCPPALCQTPIRNKDTCCHVCPEVTTSVPVRFSTSRPKARVTTTTMTNPAFITTVTMPNIVAETGEPSFGENYPSQTKMTLIYQSAAWILAGLLLAIIIFLIVALLINKKKKWVQMSCYSAPKKTVILKKHLNKNSVVYMEPSKENKFQNVNDCGIIHFSPDMSSTCREKMTIPRAKLGIANDWKPR

Target ORF information:

RefSeq Version	XM_028438331.1
Organism	Gouania willdenowi(blunt-snouted clingfish)
Definition	Gouania willdenowi cysteine rich transmembrane BMP regulator 1 (crim1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028438331.1

ORF Insert Sequence:

ATGCTCGTTC TCCTGTTGAA TGTCATCCTA ACGGTGCGGG CGCTCACAGC GCACGAGTGC 
ACGCCGTGTG ACCTCCGGAC ATGTCCAGTC AGATCCCCGC ACACATCATG TGACCATCCT 
CGGACCCTGG CCAGGGATCC GTGCGGATGC TGCGATCAGT GCGCCCGGCT GGAGTGGGAG 
CCCTGCGGGG GCCAGGATTG GGTGCTCGGG TACTGCGCCC TGGGTTTGAC GTGTGCATCT 
GTCAACAAAA CCGGAGCTGC CAGCGTCCCC GAAATTGGGG TGTGCAAAGT ACTTCCGGAT 
AATCCAGAGG CAGGTCTTGT AGATGAGCGC TGTCCGCTGG TGACAGGCTG CGACAGAGCC 
GGAGGTCAGT GTGTGTGTGA TGCCCGCCAC ACCTGCTTGG ATTCCTTCAC CTATGCAGAC 
CAAGAAACCT GCATGAAAGT CACCAAGTCA GATGGGAAAG CATCCGAGCA CCGGGAGAAG 
CACAGAGAGA AGTATGTGGG ACCCTCCAAC CCGGCCTGCA TGTTCTCGGG CTGTAACCTG 
ACTGCTGAGG GCTGTGTGTG CGAGTCCAAA AGCTGCCAGC ATCACTTCAC CTACATTAAC 
CGCAGCCAGT GCCAAGAAGT TGCAGATGAG CTGAGGTGTG CCAGGGTGAT GTGCCCTCCT 
CTGAAGGTGC CATCTTGCCC CAAGGGTTCA TTCCTGACAA AAAGCTACAC GCCCCCTGCT 
GGTTGCTGCC CCTCCATACT ACCACAGTGT ACTTGCGATC TCAGTGCCTG CCACAAACCC 
CACTGTCCAC GTGGACAGCA GCCCATTCCA CTAAATCAGG CCAGTGGGCA GCCGGGAGAC 
TGCTGTGATG TCTTTGAGTG CCAGAAAGTT TCTCCCAAGT GTATCTACAA TGAAAAGGAG 
TTTGCAGAGG GAGAGGTGTA CCGTATGGAT TCTTGCTGGT TGTGTCAGTG CCGTGGAGGA 
ATATCCTTCT GCTCCAAGGC AGAGTGTGCC GAACTGGATT GTGAAAACTT CTACATTCCA 
GAGGGCGAGT GTTGCCCTGT CTGCATAGAT GTAGAGTTGC TATCAATGGA CAGCACTAAG 
GCCAGCTGCT GGGTTGACAA CAAGCTGCGA GCCCACGAGG AGCAGTGGAA GGAGGACGAC 
TGCACCTTCT GCCAGTGTGT GGATGGAGAC CCTCACTGCA CGGCCATGGC CTGCAAACAG 
AGCTGCCAGA ACCCTGTGAA GATCCCCGGG GAGTGTTGTC CCTTCTGTGA AGAGCCTTCA 
TATGAAACCG TTAGCCCTTT GCTCTGTCCT GCAATGGAAA ACTGCTCCCT GTCAACCAAC 
GACTGCCCCT ATGGCTTCCT CCAAGACAAG AATGGATGTC TGCTATGCCA GTGTCTTAAT 
AATGATTCCT GTCCTGACCT GGAGAGATAC TGCTCACTGC AATGCCCAGT GGGATATGAA 
CGAGATGATT TTGGGTGCGA AGTGTGTGAA TGTAGTATAC CCACACCACG GTGTCGACCT 
TTGATCTGCA CCAAGACCTG CCCCTATGGA TATGTGAGGA ACAAGCACGG CTGTGAAATG 
TGTCGCTGCG TCAAGTGTCC TCCTTTCACC TGCGATAAGC ACTGCAGCAA CGGCTATCAA 
CAGAACAGAA AGGGCTGCTC CACCTGCATG TGTAAAGAAA GTCCTCATAT ACCAAGGACC 
ACTGTTCCAG CCCCGGTGCC AAGCTATTGC CTCACCTCCA ATGGGCACCG ATACGAGGAG 
GGCGACGGCT GGCATGACGG CTGTCGGGAT TGCTACTGTC ACTCTGGAAG GGAGATGTGT 
GTCCTGATAT CCTGCCCTGT CCCCAGCTGC TCTAATCCTA TAGTCAAACC AGACCAGTGT 
TGTCCTACCT GTGAAGATGA GTCGGGCAGC GGTCAGCCAG AGGGAATGGA CATGGTGGTG 
TGCAGAGCAC CTGGGGGAGA GTTCTATGTG GAAGGGGAGA CCTGGAACCT GGACGAGTGC 
ACACGGTGCA CCTGCAGAAA GGGGCGTGTC TTGTGCGACA CTGAAGTGTG TCCTCCGGCT 
CTCTGCCAGA CACCAATCAG GAACAAGGAC ACGTGTTGCC ATGTGTGCCC AGAGGTGACA 
ACATCAGTGC CCGTGAGATT TTCTACGAGT CGACCCAAAG CAAGAGTCAC TACTACTACA 
ATGACAAATC CTGCATTCAT CACTACTGTT ACAATGCCAA ACATCGTTGC TGAAACAGGT 
GAACCAAGTT TTGGTGAGAA TTACCCAAGC CAAACGAAGA TGACCCTGAT CTACCAGTCT 
GCAGCTTGGA TCCTGGCTGG ACTTCTTTTA GCCATCATTA TCTTCCTCAT CGTGGCGTTG 
CTAATCAACA AGAAGAAGAA GTGGGTGCAG ATGTCGTGCT ACAGCGCGCC AAAGAAGACC 
GTGATTCTCA AAAAGCACCT AAACAAGAAT TCGGTGGTCT ACATGGAGCC CTCCAAGGAG 
AATAAATTTC AAAATGTGAA CGACTGTGGG ATCATCCACT TCTCTCCAGA TATGAGCTCC 
ACCTGCAGGG AGAAGATGAC CATCCCCAGA GCCAAGCTGG GCATAGCAAA CGACTGGAAA 
CCTCGCTAA