MEGF6L cDNA ORF clone, Gallus gallus(chicken)

The following MEGF6L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MEGF6L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa152777 XM_025153578.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa152778 XM_025153579.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa152779 XM_025153580.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa152780 XM_025153581.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa152781 XM_025153582.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa152782 XM_025153584.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa40123 XM_015291745.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa40124 XM_015291746.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa40125 XM_015291747.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa40126 XM_015291748.2
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa40126 XM_025153583.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa152774 XM_025153568.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa152774 XM_025153565.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa152774 XM_025153571.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa152774 XM_025153573.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa152774 XM_025153569.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa152774 XM_025153564.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa152774 XM_025153570.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa152774 XM_025153567.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa152774 XM_025153572.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa152774 XM_025153574.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa152775 XM_025153575.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa152776 XM_025153577.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa40120 XM_015291733.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa40120 XM_015291734.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa40120 XM_015291742.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa40120 XM_015291741.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa40120 XM_015291735.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa40120 XM_015291737.2
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa40120 XM_015291740.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa40120 XM_015291739.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa40120 XM_015291738.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa40121 XM_015291743.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa40122 XM_015291744.1
Latest version!
Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
View Old Accession Versions >>
OGa40126 XM_015291748.1 Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa40120 XM_015291737.1 Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa152777
    Clone ID Related Accession (Same CDS sequence) XM_025153578.1
    Accession Version XM_025153578.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAAGTTGTC CCAGGGGATT TTTTGGGAAG AACTGCAACA AACCATGCAA CTGTGCCAAC
    AGCGGCCACT GCCACCGGCT ATATGGAGCC TGCCTGTGTG ACCCTGGGCT GTATGGACGC
    TTCTGCCACC TCACCTGTCC CAGGTGGGCT TTTGGTGTGG GCTGCTCACA GGAGTGTCAG
    TGTGTGAAGG AGCACACACT GGGATGCAGC GCTCGGAACG GGACCTGCAG CTGCAAGCCT
    GGATATCACG GCAAGAAGTG CCAGAAAGCA TGCACACCTG GCTTTTATGG GGCTGGTTGC
    AAACTGAGGT GCAACTGTTC CACCGACGTT TTGTGTGACC ACGAGACGGG AGCCTGCAAG
    CGTCCGTGCC CGCCTGGTTT CCATGGAGAA AAGTGCCATT CCTCTTGCCA GCCCGGGAGC
    TTTGGGGTGG ACTGCAGGCA GAGCTGCAGC TGCGGGGGAG CACCGTGTGA CCCAGAGACT
    GGGCGTTGTT TCTGCCCAGC TGGGAAAACT GGTGCCATGT GTGAGAGAGC CTGCCCTGTT
    GGATACTACG GACAAAACTG CCAGCTGCCA TGCAGCTGCA GCAGTGATGC TCTGTGTCAC
    CACATCACTG GGGAGTGCAC GTGTCCCCCC GGCTGGACTG GCCACGACTG CAAGCACCCC
    TGCAATAGCG GCCGTTGGGG ACAGCACTGT GAAAACTCCT GTGCCTGCAA TAACAGCGAT
    GGGAGCTGCG ATCCTGTCAC TGGGTCCTGC TTCTGTGAGC CAGGATTCAC TGGGAAACAC
    TGCGAGCAGA GATGTCCTGA GGGAAGCTTT GGTCCCAGCT GCATGCACAG CTGCCAGTGC
    CAGAACGGGG CTGCATGTGA CCATGTGAGC GGAGCCTGCA CGTGCAGTGC TGGATGGACC
    GGGACCTTCT GCAGGAAAGC TTGTCCAGAA GGATTTTTTG GGCTTGACTG CCACCAGGTG
    TGCAACTGCA AGAACGCTGA GGGCTGTGAC CATGTCCTGG GGACGTGCCG CTGCCTCCCA
    GGCTGGAGGG GAGAGACCTG CGAGCAGCCG TGTCAGGGGG ACAGGTTTGG GCAGGGCTGT
    GCTCACACCT GCAACTGTCA CAACGGAGCA CTCTGCGACC ACATAACGGG GACATGTGCC
    TGCACCCCAG GCTGGGGAGG AGCCACCTGC CAAGAAGCCT GTCCCCCAGG CTGGCATGGT
    GCTGGCTGCC TTCAGCGCTG CATTTGCCCT GGCCAGACTG CATGTGACCC CGTGACGGGC
    AAGTGCCAGT GTTCCAAGGG CGGAACAGGG ACATCCTGTG AGCTTGCATG TCCCCCAGGA
    TTTTTTGGAG AGAGATGTGA AGAGCAATGT GACTGCATTC ACAGCGTGTC CTGCCACCCC
    CAAACGGGGG CTTGTCGCTG TGCCAAGGGG TGGCGAGGCA GGCACTGTGA CAAACCCTGC
    TTGCCTGGCC ACTACGGCAT GGGTTGTGCC CGGCGGTGCC ACTGCCCTGC AGGCACCCCC
    TGCCACCACC TGACGGGTGA GTGCAGCTGT CCTCCAGGAT TCACTGGCCA TGGCTGCGAG
    AAAACTTGTC CACCAAACAC GTATGGCCAG AGTTGCAGCC AAGCCTGTCG CTGCCGTGCT
    GAGGAGGACT GTCACCCCAT CACTGGGCGG TGCGTGTGCC GCCTGGGGTA CCACGGGGCC
    CAGTGTGACC GCCCAGGGTG TCCAGAAGGT ACTTACGGTT CAAACTGCCA AAAATCCTGT
    AAATGCATGA ATGGAGGCCA TTGCGACCCA GTGTCGGGAA CTTGTGATTG CGCGCCTGGT
    TTCATTGGAG CCGACTGTAG TAAAACTTGC CCAGAAAATC GATACGGGAA GGACTGCGCC
    CTGTTCTGTG CATGCGGTAA AGGTCATTGT GACCCACGGA CTGGCAGCTG TACCTGTCCT
    CCTGGCCAAA TGGGACCTGG CTGTCAGCAA GTGTGTCCCC AGGGACGGTT CGGCCCCAGC
    TGCCGCCTGA CATGCAGCTG TCAGAACGGT GCTATCTGTG ACCACGTGGA TGGCAGCTGC
    ACTTGTGGAC TTGGCTGGAC AGGAAAATCG TGTGAGAAAG CAGAATGTCT CCCTGGCAAG
    TACGGCCCCA ACTGCAGCTT GGACTGCTCA TGCCAGCACA ACGGCACCTG CGACAGGTTC
    ACGGGCTGCT GCCTCTGCCC CGCGGGGTTC TACGGGCGCT CCTGTGAGCA CGGGTGCCCC
    CCCGGGTTTT ATGGGCTGAG CTGCCTTCAT GCATGTGCCT GTAAAAACGG AGCGAGCTGC
    GATGCCGTGA TGGGACAGTG CATTTGTCCC TCGGGGTACC ATGGTATCCA CTGTGAGAAA
    GGCTGTGCCA GTGGGCGCTT CGGTGAGCGG TGCCAGCGCC AGTGCGACTG TGGAGGAGCT
    CCCTGCGACC CGGCCACTGG GAAATGCCTC TGCCCACCTG GAAAGACTGG GGACAAGTGT
    GACATTGGCT GCCGGTCTGA CCAGTACGGC CCCGACTGCT CTCTGCGATG CCAGTGTGCC
    AGCAAGTCCC AGTGCAACCC ATACAATGGG AAATGTGTCT GTCCAGCCAC GTGGATGGGG
    CCAACCTGCA AGGAAGGTGG CCCTCCAAAG CTTCCTTTTT ATGAAGAACA AACTCAAGAA
    AGAGGGAATA TTTTTCCGAG AGCTCTACAA CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009346.1
    CDS179..4594
    Translation

    Target ORF information:

    RefSeq Version XM_025153578.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153578.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAAGTTGTC CCAGGGGATT TTTTGGGAAG AACTGCAACA AACCATGCAA CTGTGCCAAC
    AGCGGCCACT GCCACCGGCT ATATGGAGCC TGCCTGTGTG ACCCTGGGCT GTATGGACGC
    TTCTGCCACC TCACCTGTCC CAGGTGGGCT TTTGGTGTGG GCTGCTCACA GGAGTGTCAG
    TGTGTGAAGG AGCACACACT GGGATGCAGC GCTCGGAACG GGACCTGCAG CTGCAAGCCT
    GGATATCACG GCAAGAAGTG CCAGAAAGCA TGCACACCTG GCTTTTATGG GGCTGGTTGC
    AAACTGAGGT GCAACTGTTC CACCGACGTT TTGTGTGACC ACGAGACGGG AGCCTGCAAG
    CGTCCGTGCC CGCCTGGTTT CCATGGAGAA AAGTGCCATT CCTCTTGCCA GCCCGGGAGC
    TTTGGGGTGG ACTGCAGGCA GAGCTGCAGC TGCGGGGGAG CACCGTGTGA CCCAGAGACT
    GGGCGTTGTT TCTGCCCAGC TGGGAAAACT GGTGCCATGT GTGAGAGAGC CTGCCCTGTT
    GGATACTACG GACAAAACTG CCAGCTGCCA TGCAGCTGCA GCAGTGATGC TCTGTGTCAC
    CACATCACTG GGGAGTGCAC GTGTCCCCCC GGCTGGACTG GCCACGACTG CAAGCACCCC
    TGCAATAGCG GCCGTTGGGG ACAGCACTGT GAAAACTCCT GTGCCTGCAA TAACAGCGAT
    GGGAGCTGCG ATCCTGTCAC TGGGTCCTGC TTCTGTGAGC CAGGATTCAC TGGGAAACAC
    TGCGAGCAGA GATGTCCTGA GGGAAGCTTT GGTCCCAGCT GCATGCACAG CTGCCAGTGC
    CAGAACGGGG CTGCATGTGA CCATGTGAGC GGAGCCTGCA CGTGCAGTGC TGGATGGACC
    GGGACCTTCT GCAGGAAAGC TTGTCCAGAA GGATTTTTTG GGCTTGACTG CCACCAGGTG
    TGCAACTGCA AGAACGCTGA GGGCTGTGAC CATGTCCTGG GGACGTGCCG CTGCCTCCCA
    GGCTGGAGGG GAGAGACCTG CGAGCAGCCG TGTCAGGGGG ACAGGTTTGG GCAGGGCTGT
    GCTCACACCT GCAACTGTCA CAACGGAGCA CTCTGCGACC ACATAACGGG GACATGTGCC
    TGCACCCCAG GCTGGGGAGG AGCCACCTGC CAAGAAGCCT GTCCCCCAGG CTGGCATGGT
    GCTGGCTGCC TTCAGCGCTG CATTTGCCCT GGCCAGACTG CATGTGACCC CGTGACGGGC
    AAGTGCCAGT GTTCCAAGGG CGGAACAGGG ACATCCTGTG AGCTTGCATG TCCCCCAGGA
    TTTTTTGGAG AGAGATGTGA AGAGCAATGT GACTGCATTC ACAGCGTGTC CTGCCACCCC
    CAAACGGGGG CTTGTCGCTG TGCCAAGGGG TGGCGAGGCA GGCACTGTGA CAAACCCTGC
    TTGCCTGGCC ACTACGGCAT GGGTTGTGCC CGGCGGTGCC ACTGCCCTGC AGGCACCCCC
    TGCCACCACC TGACGGGTGA GTGCAGCTGT CCTCCAGGAT TCACTGGCCA TGGCTGCGAG
    AAAACTTGTC CACCAAACAC GTATGGCCAG AGTTGCAGCC AAGCCTGTCG CTGCCGTGCT
    GAGGAGGACT GTCACCCCAT CACTGGGCGG TGCGTGTGCC GCCTGGGGTA CCACGGGGCC
    CAGTGTGACC GCCCAGGGTG TCCAGAAGGT ACTTACGGTT CAAACTGCCA AAAATCCTGT
    AAATGCATGA ATGGAGGCCA TTGCGACCCA GTGTCGGGAA CTTGTGATTG CGCGCCTGGT
    TTCATTGGAG CCGACTGTAG TAAAACTTGC CCAGAAAATC GATACGGGAA GGACTGCGCC
    CTGTTCTGTG CATGCGGTAA AGGTCATTGT GACCCACGGA CTGGCAGCTG TACCTGTCCT
    CCTGGCCAAA TGGGACCTGG CTGTCAGCAA GTGTGTCCCC AGGGACGGTT CGGCCCCAGC
    TGCCGCCTGA CATGCAGCTG TCAGAACGGT GCTATCTGTG ACCACGTGGA TGGCAGCTGC
    ACTTGTGGAC TTGGCTGGAC AGGAAAATCG TGTGAGAAAG CAGAATGTCT CCCTGGCAAG
    TACGGCCCCA ACTGCAGCTT GGACTGCTCA TGCCAGCACA ACGGCACCTG CGACAGGTTC
    ACGGGCTGCT GCCTCTGCCC CGCGGGGTTC TACGGGCGCT CCTGTGAGCA CGGGTGCCCC
    CCCGGGTTTT ATGGGCTGAG CTGCCTTCAT GCATGTGCCT GTAAAAACGG AGCGAGCTGC
    GATGCCGTGA TGGGACAGTG CATTTGTCCC TCGGGGTACC ATGGTATCCA CTGTGAGAAA
    GGCTGTGCCA GTGGGCGCTT CGGTGAGCGG TGCCAGCGCC AGTGCGACTG TGGAGGAGCT
    CCCTGCGACC CGGCCACTGG GAAATGCCTC TGCCCACCTG GAAAGACTGG GGACAAGTGT
    GACATTGGCT GCCGGTCTGA CCAGTACGGC CCCGACTGCT CTCTGCGATG CCAGTGTGCC
    AGCAAGTCCC AGTGCAACCC ATACAATGGG AAATGTGTCT GTCCAGCCAC GTGGATGGGG
    CCAACCTGCA AGGAAGGTGG CCCTCCAAAG CTTCCTTTTT ATGAAGAACA AACTCAAGAA
    AGAGGGAATA TTTTTCCGAG AGCTCTACAA CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152778
    Clone ID Related Accession (Same CDS sequence) XM_025153579.1
    Accession Version XM_025153579.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCATG TCCCCCAGGA
    TTTTTTGGAG AGAGATGTGA AGAGCAATGT GACTGCATTC ACAGCGTGTC CTGCCACCCC
    CAAACGGGGG CTTGTCGCTG TGCCAAGGGG TGGCGAGGCA GGCACTGTGA CAAACCCTGC
    TTGCCTGGCC ACTACGGCAT GGGTTGTGCC CGGCGGTGCC ACTGCCCTGC AGGCACCCCC
    TGCCACCACC TGACGGGTGA GTGCAGCTGT CCTCCAGGAT TCACTGGCCA TGGCTGCGAG
    AAAACTTGTC CACCAAACAC GTATGGCCAG AGTTGCAGCC AAGCCTGTCG CTGCCGTGCT
    GAGGAGGACT GTCACCCCAT CACTGGGCGG TGCGTGTGCC GCCTGGGGTA CCACGGGGCC
    CAGTGTGACC GCCCAGGGTG TCCAGAAGGT ACTTACGGTT CAAACTGCCA AAAATCCTGT
    AAATGCATGA ATGGAGGCCA TTGCGACCCA GTGTCGGGAA CTTGTGATTG CGCGCCTGGT
    TTCATTGGAG CCGACTGTAG TAAAACTTGC CCAGAAAATC GATACGGGAA GGACTGCGCC
    CTGTTCTGTG CATGCGGTAA AGGTCATTGT GACCCACGGA CTGGCAGCTG TACCTGTCCT
    CCTGGCCAAA TGGGACCTGG CTGTCAGCAA GTGTGTCCCC AGGGACGGTT CGGCCCCAGC
    TGCCGCCTGA CATGCAGCTG TCAGAACGGT GCTATCTGTG ACCACGTGGA TGGCAGCTGC
    ACTTGTGGAC TTGGCTGGAC AGGAAAATCG TGTGAGAAAG CAGAATGTCT CCCTGGCAAG
    TACGGCCCCA ACTGCAGCTT GGACTGCTCA TGCCAGCACA ACGGCACCTG CGACAGGTTC
    ACGGGCTGCT GCCTCTGCCC CGCGGGGTTC TACGGGCGCT CCTGTGAGCA CGGGTGCCCC
    CCCGGGTTTT ATGGGCTGAG CTGCCTTCAT GCATGTGCCT GTAAAAACGG AGCGAGCTGC
    GATGCCGTGA TGGGACAGTG CATTTGTCCC TCGGGGTACC ATGGTATCCA CTGTGAGAAA
    GGCTGTGCCA GTGGGCGCTT CGGTGAGCGG TGCCAGCGCC AGTGCGACTG TGGAGGAGCT
    CCCTGCGACC CGGCCACTGG GAAATGCCTC TGCCCACCTG GAAAGACTGG GGACAAGTGT
    GACATTGGCT GCCGGTCTGA CCAGTACGGC CCCGACTGCT CTCTGCGATG CCAGTGTGCC
    AGCAAGTCCC AGTGCAACCC ATACAATGGG AAATGTGTCT GTCCAGCCAC GTGGATGGGG
    CCAACCTGCA AGGAAGGTGG CCCTCCAAAG CTTCCTTTTT ATGAAGAACA AACTCAAGAA
    AGAGGGAATA TTTTTCCGAG AGCTCTACAA CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009347.1
    CDS180..4595
    Translation

    Target ORF information:

    RefSeq Version XM_025153579.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153579.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCATG TCCCCCAGGA
    TTTTTTGGAG AGAGATGTGA AGAGCAATGT GACTGCATTC ACAGCGTGTC CTGCCACCCC
    CAAACGGGGG CTTGTCGCTG TGCCAAGGGG TGGCGAGGCA GGCACTGTGA CAAACCCTGC
    TTGCCTGGCC ACTACGGCAT GGGTTGTGCC CGGCGGTGCC ACTGCCCTGC AGGCACCCCC
    TGCCACCACC TGACGGGTGA GTGCAGCTGT CCTCCAGGAT TCACTGGCCA TGGCTGCGAG
    AAAACTTGTC CACCAAACAC GTATGGCCAG AGTTGCAGCC AAGCCTGTCG CTGCCGTGCT
    GAGGAGGACT GTCACCCCAT CACTGGGCGG TGCGTGTGCC GCCTGGGGTA CCACGGGGCC
    CAGTGTGACC GCCCAGGGTG TCCAGAAGGT ACTTACGGTT CAAACTGCCA AAAATCCTGT
    AAATGCATGA ATGGAGGCCA TTGCGACCCA GTGTCGGGAA CTTGTGATTG CGCGCCTGGT
    TTCATTGGAG CCGACTGTAG TAAAACTTGC CCAGAAAATC GATACGGGAA GGACTGCGCC
    CTGTTCTGTG CATGCGGTAA AGGTCATTGT GACCCACGGA CTGGCAGCTG TACCTGTCCT
    CCTGGCCAAA TGGGACCTGG CTGTCAGCAA GTGTGTCCCC AGGGACGGTT CGGCCCCAGC
    TGCCGCCTGA CATGCAGCTG TCAGAACGGT GCTATCTGTG ACCACGTGGA TGGCAGCTGC
    ACTTGTGGAC TTGGCTGGAC AGGAAAATCG TGTGAGAAAG CAGAATGTCT CCCTGGCAAG
    TACGGCCCCA ACTGCAGCTT GGACTGCTCA TGCCAGCACA ACGGCACCTG CGACAGGTTC
    ACGGGCTGCT GCCTCTGCCC CGCGGGGTTC TACGGGCGCT CCTGTGAGCA CGGGTGCCCC
    CCCGGGTTTT ATGGGCTGAG CTGCCTTCAT GCATGTGCCT GTAAAAACGG AGCGAGCTGC
    GATGCCGTGA TGGGACAGTG CATTTGTCCC TCGGGGTACC ATGGTATCCA CTGTGAGAAA
    GGCTGTGCCA GTGGGCGCTT CGGTGAGCGG TGCCAGCGCC AGTGCGACTG TGGAGGAGCT
    CCCTGCGACC CGGCCACTGG GAAATGCCTC TGCCCACCTG GAAAGACTGG GGACAAGTGT
    GACATTGGCT GCCGGTCTGA CCAGTACGGC CCCGACTGCT CTCTGCGATG CCAGTGTGCC
    AGCAAGTCCC AGTGCAACCC ATACAATGGG AAATGTGTCT GTCCAGCCAC GTGGATGGGG
    CCAACCTGCA AGGAAGGTGG CCCTCCAAAG CTTCCTTTTT ATGAAGAACA AACTCAAGAA
    AGAGGGAATA TTTTTCCGAG AGCTCTACAA CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152779
    Clone ID Related Accession (Same CDS sequence) XM_025153580.1
    Accession Version XM_025153580.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4395bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGGAGCGG TGTGGCTGTG CCTCGGGCTG GGCTGGGCGC TGGCGGCCCG CGTGAGCAGA 
    GCGGAGGAGC CGAGACTGCA GAGGGGCATG CCAAATGTCT GCCCTGTGTT GGAGCTGGTG
    CTGGTGGGAC ACCAACAACC ATGTGTTCAG GCCTTCAGCC GCATGGTGAA GATGTGGAAA
    CAAGGCTGCA CAGGGAGGAG ATGGTGCATG GGATATGAGA GGAGGTCTGG TTACTACACT
    GTGTATAAGC AGGTGTACAG AATGGAGCAC CAGACTGTCT ATAAGTGCTG CCCTGGGTGG
    GCTCAACAGA ACAGCGAGCT TGGCTGCTTG CACATGCTCT GTGCTGCTGA CACTTGCTTC
    AATGGAGGGA AGTGTTCTGA AGAAGGACCT CACATGTGCC AGTGTCCTGC GGGATTTCAG
    GGTCCTCGCT GCCAGTATGA TGTAAATGAG TGCAGCACTG AGAACGGAGG CTGTCAGGAC
    CAGTGCTGTA ACACCATCGG CAGCTACCAC TGCAAATGTC CAGCTGGCCA GAAACTGGGG
    GAAGATGGAA AATCGTGCGG AGATATAGAT GAGTGTGCAG AGGTGAATGG AGGCTGCCAG
    CAGGGCTGCA TCAACACCCA CAGCTCTTTC CACTGCCACT GTTGGGCAGG ATACAGGCTG
    CACTCTGATG GGCGCACGTG CTTGATGATA GACCCCTGCA CAAGTGGGAA TGGAGGATGC
    TCACAAATCT GTCAAAATGA GAGAGGAACT GCCAAATGTG ACTGTCACCC AGGGCATTAT
    CTTTCTACAG ATGAGAAGTC CTGCTTAGAC ATTGATGAGT GTGCAGAAGG GAAAGCCAGG
    TGTGCTCATC ACTGTGTGAA CATGCTGGGA TCCTTCACCT GTGCCTGTAA TCCCGGCTTC
    GAGCTGGGGG CTGATGGGAA GCAGTGCTAC CGCATTGAAA TGGAGATAGT GGACAGCTGC
    CAGCAGGACA ACGGTGGGTG CTCCCACCGC TGCGAGCACA CAGCAGCAGG ACCACGCTGC
    TCCTGCCACG ATGGCTACGT GCTCAGCTCA GATGGCAAGG CATGCACAGA CCTGGATGAG
    TGCGAGACCG GCGAGTCGTG CTGCTCCCAG TTCTGCATCA ACTACGTGGG CAGCTACGAG
    TGCACCTGTA GAGCAGGCTT CCAGCCCAGT GCCGATGGCT GCAGCTGCGA CGATGTGGAT
    GAATGTCGTC TTGAGAATGG CAACTGTGAC CATATCTGCA TTAATTCCCT GGGGTCATAC
    GAATGCGCCT GCAAGGAGAG GTACCGGCTG GGTGCTGACC GGCGCTCCTG CATTGTGTTA
    GGAGGGGACT CAGCAGTGTC GGGCTTCGCA GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC
    CCCCAGCTCC TGCACTATGT GCTTGGGGCC CCACGTGAGG ATGAGGAGCA GCGAGGAGAG
    CTGACACTGA TACACAGAGC CGTTTGCCTT GATAACACTT TTGGCAATGA CTGCAGCTTG
    AGCTGTGAGG ATTGTATGAA TGGAGGCAGA TGTAACAGTG AGAACAGTGG CTGTGTTTGC
    TCACCTGGGT GGACCGGCAT CATCTGCAAC CAAACCTGCG CCCAGGGTAC ATACGGTGAT
    GGTTGTGCCA GTATCTGCAT GTGCCAGAAT GGAGGCTCCT GTGACCCCAT CACAGGGCTT
    TGTCACTGCC CACCAGGAGT CCAGGGGCCA TTTTGTGAAG ACGGTTGTCC CAGGGGATTT
    TTTGGGAAGA ACTGCAACAA ACCATGCAAC TGTGCCAACA GCGGCCACTG CCACCGGCTA
    TATGGAGCCT GCCTGTGTGA CCCTGGGCTG TATGGACGCT TCTGCCACCT CACCTGTCCC
    AGGTGGGCTT TTGGTGTGGG CTGCTCACAG GAGTGTCAGT GTGTGAAGGA GCACACACTG
    GGATGCAGCG CTCGGAACGG GACCTGCAGC TGCAAGCCTG GATATCACGG CAAGAAGTGC
    CAGAAAGCAT GCACACCTGG CTTTTATGGG GCTGGTTGCA AACTGAGGTG CAACTGTTCC
    ACCGACGTTT TGTGTGACCA CGAGACGGGA GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC
    CATGGAGAAA AGTGCCATTC CTCTTGCCAG CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG
    AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC CCAGAGACTG GGCGTTGTTT CTGCCCAGCT
    GGGAAAACTG GTGCCATGTG TGAGAGAGCC TGCCCTGTTG GATACTACGG ACAAAACTGC
    CAGCTGCCAT GCAGCTGCAG CAGTGATGCT CTGTGTCACC ACATCACTGG GGAGTGCACG
    TGTCCCCCCG GCTGGACTGG CCACGACTGC AAGCACCCCT GCAATAGCGG CCGTTGGGGA
    CAGCACTGTG AAAACTCCTG TGCCTGCAAT AACAGCGATG GGAGCTGCGA TCCTGTCACT
    GGGTCCTGCT TCTGTGAGCC AGGATTCACT GGGAAACACT GCGAGCAGAG ATGTCCTGAG
    GGAAGCTTTG GTCCCAGCTG CATGCACAGC TGCCAGTGCC AGAACGGGGC TGCATGTGAC
    CATGTGAGCG GAGCCTGCAC GTGCAGTGCT GGATGGACCG GGACCTTCTG CAGGAAAGCT
    TGTCCAGAAG GATTTTTTGG GCTTGACTGC CACCAGGTGT GCAACTGCAA GAACGCTGAG
    GGCTGTGACC ATGTCCTGGG GACGTGCCGC TGCCTCCCAG GCTGGAGGGG AGAGACCTGC
    GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG CAGGGCTGTG CTCACACCTG CAACTGTCAC
    AACGGAGCAC TCTGCGACCA CATAACGGGG ACATGTGCCT GCACCCCAGG CTGGGGAGGA
    GCCACCTGCC AAGAAGCCTG TCCCCCAGGC TGGCATGGTG CTGGCTGCCT TCAGCGCTGC
    ATTTGCCCTG GCCAGACTGC ATGTGACCCC GTGACGGGCA AGTGCCAGTG TTCCAAGGGC
    GGAACAGGGA CATCCTGTGA GCTTGCATGT CCCCCAGGAT TTTTTGGAGA GAGATGTGAA
    GAGCAATGTG ACTGCATTCA CAGCGTGTCC TGCCACCCCC AAACGGGGGC TTGTCGCTGT
    GCCAAGGGGT GGCGAGGCAG GCACTGTGAC AAACCCTGCT TGCCTGGCCA CTACGGCATG
    GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA GGCACCCCCT GCCACCACCT GACGGGTGAG
    TGCAGCTGTC CTCCAGGATT CACTGGCCAT GGCTGCGAGA AAACTTGTCC ACCAAACACG
    TATGGCCAGA GTTGCAGCCA AGCCTGTCGC TGCCGTGCTG AGGAGGACTG TCACCCCATC
    ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC CACGGGGCCC AGTGTGACCG CCCAGGGTGT
    CCAGAAGGTA CTTACGGTTC AAACTGCCAA AAATCCTGTA AATGCATGAA TGGAGGCCAT
    TGCGACCCAG TGTCGGGAAC TTGTGATTGC GCGCCTGGTT TCATTGGAGC CGACTGTAGT
    AAAACTTGCC CAGAAAATCG ATACGGGAAG GACTGCGCCC TGTTCTGTGC ATGCGGTAAA
    GGTCATTGTG ACCCACGGAC TGGCAGCTGT ACCTGTCCTC CTGGCCAAAT GGGACCTGGC
    TGTCAGCAAG TGTGTCCCCA GGGACGGTTC GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT
    CAGAACGGTG CTATCTGTGA CCACGTGGAT GGCAGCTGCA CTTGTGGACT TGGCTGGACA
    GGAAAATCGT GTGAGAAAGC AGAATGTCTC CCTGGCAAGT ACGGCCCCAA CTGCAGCTTG
    GACTGCTCAT GCCAGCACAA CGGCACCTGC GACAGGTTCA CGGGCTGCTG CCTCTGCCCC
    GCGGGGTTCT ACGGGCGCTC CTGTGAGCAC GGGTGCCCCC CCGGGTTTTA TGGGCTGAGC
    TGCCTTCATG CATGTGCCTG TAAAAACGGA GCGAGCTGCG ATGCCGTGAT GGGACAGTGC
    ATTTGTCCCT CGGGGTACCA TGGTATCCAC TGTGAGAAAG GCTGTGCCAG TGGGCGCTTC
    GGTGAGCGGT GCCAGCGCCA GTGCGACTGT GGAGGAGCTC CCTGCGACCC GGCCACTGGG
    AAATGCCTCT GCCCACCTGG AAAGACTGGG GACAAGTGTG ACATTGGCTG CCGGTCTGAC
    CAGTACGGCC CCGACTGCTC TCTGCGATGC CAGTGTGCCA GCAAGTCCCA GTGCAACCCA
    TACAATGGGA AATGTGTCTG TCCAGCCACG TGGATGGGGC CAACCTGCAA GGAAGGTGGC
    CCTCCAAAGC TTCCTTTTTA TGAAGAACAA ACTCAAGAAA GAGGGAATAT TTTTCCGAGA
    GCTCTACAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009348.1
    CDS187..4581
    Translation

    Target ORF information:

    RefSeq Version XM_025153580.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153580.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGGAGCGG TGTGGCTGTG CCTCGGGCTG GGCTGGGCGC TGGCGGCCCG CGTGAGCAGA 
    GCGGAGGAGC CGAGACTGCA GAGGGGCATG CCAAATGTCT GCCCTGTGTT GGAGCTGGTG
    CTGGTGGGAC ACCAACAACC ATGTGTTCAG GCCTTCAGCC GCATGGTGAA GATGTGGAAA
    CAAGGCTGCA CAGGGAGGAG ATGGTGCATG GGATATGAGA GGAGGTCTGG TTACTACACT
    GTGTATAAGC AGGTGTACAG AATGGAGCAC CAGACTGTCT ATAAGTGCTG CCCTGGGTGG
    GCTCAACAGA ACAGCGAGCT TGGCTGCTTG CACATGCTCT GTGCTGCTGA CACTTGCTTC
    AATGGAGGGA AGTGTTCTGA AGAAGGACCT CACATGTGCC AGTGTCCTGC GGGATTTCAG
    GGTCCTCGCT GCCAGTATGA TGTAAATGAG TGCAGCACTG AGAACGGAGG CTGTCAGGAC
    CAGTGCTGTA ACACCATCGG CAGCTACCAC TGCAAATGTC CAGCTGGCCA GAAACTGGGG
    GAAGATGGAA AATCGTGCGG AGATATAGAT GAGTGTGCAG AGGTGAATGG AGGCTGCCAG
    CAGGGCTGCA TCAACACCCA CAGCTCTTTC CACTGCCACT GTTGGGCAGG ATACAGGCTG
    CACTCTGATG GGCGCACGTG CTTGATGATA GACCCCTGCA CAAGTGGGAA TGGAGGATGC
    TCACAAATCT GTCAAAATGA GAGAGGAACT GCCAAATGTG ACTGTCACCC AGGGCATTAT
    CTTTCTACAG ATGAGAAGTC CTGCTTAGAC ATTGATGAGT GTGCAGAAGG GAAAGCCAGG
    TGTGCTCATC ACTGTGTGAA CATGCTGGGA TCCTTCACCT GTGCCTGTAA TCCCGGCTTC
    GAGCTGGGGG CTGATGGGAA GCAGTGCTAC CGCATTGAAA TGGAGATAGT GGACAGCTGC
    CAGCAGGACA ACGGTGGGTG CTCCCACCGC TGCGAGCACA CAGCAGCAGG ACCACGCTGC
    TCCTGCCACG ATGGCTACGT GCTCAGCTCA GATGGCAAGG CATGCACAGA CCTGGATGAG
    TGCGAGACCG GCGAGTCGTG CTGCTCCCAG TTCTGCATCA ACTACGTGGG CAGCTACGAG
    TGCACCTGTA GAGCAGGCTT CCAGCCCAGT GCCGATGGCT GCAGCTGCGA CGATGTGGAT
    GAATGTCGTC TTGAGAATGG CAACTGTGAC CATATCTGCA TTAATTCCCT GGGGTCATAC
    GAATGCGCCT GCAAGGAGAG GTACCGGCTG GGTGCTGACC GGCGCTCCTG CATTGTGTTA
    GGAGGGGACT CAGCAGTGTC GGGCTTCGCA GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC
    CCCCAGCTCC TGCACTATGT GCTTGGGGCC CCACGTGAGG ATGAGGAGCA GCGAGGAGAG
    CTGACACTGA TACACAGAGC CGTTTGCCTT GATAACACTT TTGGCAATGA CTGCAGCTTG
    AGCTGTGAGG ATTGTATGAA TGGAGGCAGA TGTAACAGTG AGAACAGTGG CTGTGTTTGC
    TCACCTGGGT GGACCGGCAT CATCTGCAAC CAAACCTGCG CCCAGGGTAC ATACGGTGAT
    GGTTGTGCCA GTATCTGCAT GTGCCAGAAT GGAGGCTCCT GTGACCCCAT CACAGGGCTT
    TGTCACTGCC CACCAGGAGT CCAGGGGCCA TTTTGTGAAG ACGGTTGTCC CAGGGGATTT
    TTTGGGAAGA ACTGCAACAA ACCATGCAAC TGTGCCAACA GCGGCCACTG CCACCGGCTA
    TATGGAGCCT GCCTGTGTGA CCCTGGGCTG TATGGACGCT TCTGCCACCT CACCTGTCCC
    AGGTGGGCTT TTGGTGTGGG CTGCTCACAG GAGTGTCAGT GTGTGAAGGA GCACACACTG
    GGATGCAGCG CTCGGAACGG GACCTGCAGC TGCAAGCCTG GATATCACGG CAAGAAGTGC
    CAGAAAGCAT GCACACCTGG CTTTTATGGG GCTGGTTGCA AACTGAGGTG CAACTGTTCC
    ACCGACGTTT TGTGTGACCA CGAGACGGGA GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC
    CATGGAGAAA AGTGCCATTC CTCTTGCCAG CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG
    AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC CCAGAGACTG GGCGTTGTTT CTGCCCAGCT
    GGGAAAACTG GTGCCATGTG TGAGAGAGCC TGCCCTGTTG GATACTACGG ACAAAACTGC
    CAGCTGCCAT GCAGCTGCAG CAGTGATGCT CTGTGTCACC ACATCACTGG GGAGTGCACG
    TGTCCCCCCG GCTGGACTGG CCACGACTGC AAGCACCCCT GCAATAGCGG CCGTTGGGGA
    CAGCACTGTG AAAACTCCTG TGCCTGCAAT AACAGCGATG GGAGCTGCGA TCCTGTCACT
    GGGTCCTGCT TCTGTGAGCC AGGATTCACT GGGAAACACT GCGAGCAGAG ATGTCCTGAG
    GGAAGCTTTG GTCCCAGCTG CATGCACAGC TGCCAGTGCC AGAACGGGGC TGCATGTGAC
    CATGTGAGCG GAGCCTGCAC GTGCAGTGCT GGATGGACCG GGACCTTCTG CAGGAAAGCT
    TGTCCAGAAG GATTTTTTGG GCTTGACTGC CACCAGGTGT GCAACTGCAA GAACGCTGAG
    GGCTGTGACC ATGTCCTGGG GACGTGCCGC TGCCTCCCAG GCTGGAGGGG AGAGACCTGC
    GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG CAGGGCTGTG CTCACACCTG CAACTGTCAC
    AACGGAGCAC TCTGCGACCA CATAACGGGG ACATGTGCCT GCACCCCAGG CTGGGGAGGA
    GCCACCTGCC AAGAAGCCTG TCCCCCAGGC TGGCATGGTG CTGGCTGCCT TCAGCGCTGC
    ATTTGCCCTG GCCAGACTGC ATGTGACCCC GTGACGGGCA AGTGCCAGTG TTCCAAGGGC
    GGAACAGGGA CATCCTGTGA GCTTGCATGT CCCCCAGGAT TTTTTGGAGA GAGATGTGAA
    GAGCAATGTG ACTGCATTCA CAGCGTGTCC TGCCACCCCC AAACGGGGGC TTGTCGCTGT
    GCCAAGGGGT GGCGAGGCAG GCACTGTGAC AAACCCTGCT TGCCTGGCCA CTACGGCATG
    GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA GGCACCCCCT GCCACCACCT GACGGGTGAG
    TGCAGCTGTC CTCCAGGATT CACTGGCCAT GGCTGCGAGA AAACTTGTCC ACCAAACACG
    TATGGCCAGA GTTGCAGCCA AGCCTGTCGC TGCCGTGCTG AGGAGGACTG TCACCCCATC
    ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC CACGGGGCCC AGTGTGACCG CCCAGGGTGT
    CCAGAAGGTA CTTACGGTTC AAACTGCCAA AAATCCTGTA AATGCATGAA TGGAGGCCAT
    TGCGACCCAG TGTCGGGAAC TTGTGATTGC GCGCCTGGTT TCATTGGAGC CGACTGTAGT
    AAAACTTGCC CAGAAAATCG ATACGGGAAG GACTGCGCCC TGTTCTGTGC ATGCGGTAAA
    GGTCATTGTG ACCCACGGAC TGGCAGCTGT ACCTGTCCTC CTGGCCAAAT GGGACCTGGC
    TGTCAGCAAG TGTGTCCCCA GGGACGGTTC GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT
    CAGAACGGTG CTATCTGTGA CCACGTGGAT GGCAGCTGCA CTTGTGGACT TGGCTGGACA
    GGAAAATCGT GTGAGAAAGC AGAATGTCTC CCTGGCAAGT ACGGCCCCAA CTGCAGCTTG
    GACTGCTCAT GCCAGCACAA CGGCACCTGC GACAGGTTCA CGGGCTGCTG CCTCTGCCCC
    GCGGGGTTCT ACGGGCGCTC CTGTGAGCAC GGGTGCCCCC CCGGGTTTTA TGGGCTGAGC
    TGCCTTCATG CATGTGCCTG TAAAAACGGA GCGAGCTGCG ATGCCGTGAT GGGACAGTGC
    ATTTGTCCCT CGGGGTACCA TGGTATCCAC TGTGAGAAAG GCTGTGCCAG TGGGCGCTTC
    GGTGAGCGGT GCCAGCGCCA GTGCGACTGT GGAGGAGCTC CCTGCGACCC GGCCACTGGG
    AAATGCCTCT GCCCACCTGG AAAGACTGGG GACAAGTGTG ACATTGGCTG CCGGTCTGAC
    CAGTACGGCC CCGACTGCTC TCTGCGATGC CAGTGTGCCA GCAAGTCCCA GTGCAACCCA
    TACAATGGGA AATGTGTCTG TCCAGCCACG TGGATGGGGC CAACCTGCAA GGAAGGTGGC
    CCTCCAAAGC TTCCTTTTTA TGAAGAACAA ACTCAAGAAA GAGGGAATAT TTTTCCGAGA
    GCTCTACAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152780
    Clone ID Related Accession (Same CDS sequence) XM_025153581.1
    Accession Version XM_025153581.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4383bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCTGAGC CTGCGGTGCG ATGTGTTCCC GGGCAGTATC AGGCCAATAC ACACAAACGG 
    CCTTCAGCAG ACTTCTGGCC AAATGTCTGC CCTGTGTTGG AGCTGGTGCT GGTGGGACAC
    CAACAACCAT GTGTTCAGGC CTTCAGCCGC ATGGTGAAGA TGTGGAAACA AGGCTGCACA
    GGGAGGAGAT GGTGCATGGG ATATGAGAGG AGGTCTGGTT ACTACACTGT GTATAAGCAG
    GTGTACAGAA TGGAGCACCA GACTGTCTAT AAGTGCTGCC CTGGGTGGGC TCAACAGAAC
    AGCGAGCTTG GCTGCTTGCA CATGCTCTGT GCTGCTGACA CTTGCTTCAA TGGAGGGAAG
    TGTTCTGAAG AAGGACCTCA CATGTGCCAG TGTCCTGCGG GATTTCAGGG TCCTCGCTGC
    CAGTATGATG TAAATGAGTG CAGCACTGAG AACGGAGGCT GTCAGGACCA GTGCTGTAAC
    ACCATCGGCA GCTACCACTG CAAATGTCCA GCTGGCCAGA AACTGGGGGA AGATGGAAAA
    TCGTGCGGAG ATATAGATGA GTGTGCAGAG GTGAATGGAG GCTGCCAGCA GGGCTGCATC
    AACACCCACA GCTCTTTCCA CTGCCACTGT TGGGCAGGAT ACAGGCTGCA CTCTGATGGG
    CGCACGTGCT TGATGATAGA CCCCTGCACA AGTGGGAATG GAGGATGCTC ACAAATCTGT
    CAAAATGAGA GAGGAACTGC CAAATGTGAC TGTCACCCAG GGCATTATCT TTCTACAGAT
    GAGAAGTCCT GCTTAGACAT TGATGAGTGT GCAGAAGGGA AAGCCAGGTG TGCTCATCAC
    TGTGTGAACA TGCTGGGATC CTTCACCTGT GCCTGTAATC CCGGCTTCGA GCTGGGGGCT
    GATGGGAAGC AGTGCTACCG CATTGAAATG GAGATAGTGG ACAGCTGCCA GCAGGACAAC
    GGTGGGTGCT CCCACCGCTG CGAGCACACA GCAGCAGGAC CACGCTGCTC CTGCCACGAT
    GGCTACGTGC TCAGCTCAGA TGGCAAGGCA TGCACAGACC TGGATGAGTG CGAGACCGGC
    GAGTCGTGCT GCTCCCAGTT CTGCATCAAC TACGTGGGCA GCTACGAGTG CACCTGTAGA
    GCAGGCTTCC AGCCCAGTGC CGATGGCTGC AGCTGCGACG ATGTGGATGA ATGTCGTCTT
    GAGAATGGCA ACTGTGACCA TATCTGCATT AATTCCCTGG GGTCATACGA ATGCGCCTGC
    AAGGAGAGGT ACCGGCTGGG TGCTGACCGG CGCTCCTGCA TTGTGTTAGG AGGGGACTCA
    GCAGTGTCGG GCTTCGCAGG GGCTCCGGGG CTGCAGTTCA GAGGTGCCCC CCAGCTCCTG
    CACTATGTGC TTGGGGCCCC ACGTGAGGAT GAGGAGCAGC GAGGAGAGCT GACACTGATA
    CACAGAGCCG TTTGCCTTGA TAACACTTTT GGCAATGACT GCAGCTTGAG CTGTGAGGAT
    TGTATGAATG GAGGCAGATG TAACAGTGAG AACAGTGGCT GTGTTTGCTC ACCTGGGTGG
    ACCGGCATCA TCTGCAACCA AACCTGCGCC CAGGGTACAT ACGGTGATGG TTGTGCCAGT
    ATCTGCATGT GCCAGAATGG AGGCTCCTGT GACCCCATCA CAGGGCTTTG TCACTGCCCA
    CCAGGAGTCC AGGGGCCATT TTGTGAAGAC GGTTGTCCCA GGGGATTTTT TGGGAAGAAC
    TGCAACAAAC CATGCAACTG TGCCAACAGC GGCCACTGCC ACCGGCTATA TGGAGCCTGC
    CTGTGTGACC CTGGGCTGTA TGGACGCTTC TGCCACCTCA CCTGTCCCAG GTGGGCTTTT
    GGTGTGGGCT GCTCACAGGA GTGTCAGTGT GTGAAGGAGC ACACACTGGG ATGCAGCGCT
    CGGAACGGGA CCTGCAGCTG CAAGCCTGGA TATCACGGCA AGAAGTGCCA GAAAGCATGC
    ACACCTGGCT TTTATGGGGC TGGTTGCAAA CTGAGGTGCA ACTGTTCCAC CGACGTTTTG
    TGTGACCACG AGACGGGAGC CTGCAAGCGT CCGTGCCCGC CTGGTTTCCA TGGAGAAAAG
    TGCCATTCCT CTTGCCAGCC CGGGAGCTTT GGGGTGGACT GCAGGCAGAG CTGCAGCTGC
    GGGGGAGCAC CGTGTGACCC AGAGACTGGG CGTTGTTTCT GCCCAGCTGG GAAAACTGGT
    GCCATGTGTG AGAGAGCCTG CCCTGTTGGA TACTACGGAC AAAACTGCCA GCTGCCATGC
    AGCTGCAGCA GTGATGCTCT GTGTCACCAC ATCACTGGGG AGTGCACGTG TCCCCCCGGC
    TGGACTGGCC ACGACTGCAA GCACCCCTGC AATAGCGGCC GTTGGGGACA GCACTGTGAA
    AACTCCTGTG CCTGCAATAA CAGCGATGGG AGCTGCGATC CTGTCACTGG GTCCTGCTTC
    TGTGAGCCAG GATTCACTGG GAAACACTGC GAGCAGAGAT GTCCTGAGGG AAGCTTTGGT
    CCCAGCTGCA TGCACAGCTG CCAGTGCCAG AACGGGGCTG CATGTGACCA TGTGAGCGGA
    GCCTGCACGT GCAGTGCTGG ATGGACCGGG ACCTTCTGCA GGAAAGCTTG TCCAGAAGGA
    TTTTTTGGGC TTGACTGCCA CCAGGTGTGC AACTGCAAGA ACGCTGAGGG CTGTGACCAT
    GTCCTGGGGA CGTGCCGCTG CCTCCCAGGC TGGAGGGGAG AGACCTGCGA GCAGCCGTGT
    CAGGGGGACA GGTTTGGGCA GGGCTGTGCT CACACCTGCA ACTGTCACAA CGGAGCACTC
    TGCGACCACA TAACGGGGAC ATGTGCCTGC ACCCCAGGCT GGGGAGGAGC CACCTGCCAA
    GAAGCCTGTC CCCCAGGCTG GCATGGTGCT GGCTGCCTTC AGCGCTGCAT TTGCCCTGGC
    CAGACTGCAT GTGACCCCGT GACGGGCAAG TGCCAGTGTT CCAAGGGCGG AACAGGGACA
    TCCTGTGAGC TTGCATGTCC CCCAGGATTT TTTGGAGAGA GATGTGAAGA GCAATGTGAC
    TGCATTCACA GCGTGTCCTG CCACCCCCAA ACGGGGGCTT GTCGCTGTGC CAAGGGGTGG
    CGAGGCAGGC ACTGTGACAA ACCCTGCTTG CCTGGCCACT ACGGCATGGG TTGTGCCCGG
    CGGTGCCACT GCCCTGCAGG CACCCCCTGC CACCACCTGA CGGGTGAGTG CAGCTGTCCT
    CCAGGATTCA CTGGCCATGG CTGCGAGAAA ACTTGTCCAC CAAACACGTA TGGCCAGAGT
    TGCAGCCAAG CCTGTCGCTG CCGTGCTGAG GAGGACTGTC ACCCCATCAC TGGGCGGTGC
    GTGTGCCGCC TGGGGTACCA CGGGGCCCAG TGTGACCGCC CAGGGTGTCC AGAAGGTACT
    TACGGTTCAA ACTGCCAAAA ATCCTGTAAA TGCATGAATG GAGGCCATTG CGACCCAGTG
    TCGGGAACTT GTGATTGCGC GCCTGGTTTC ATTGGAGCCG ACTGTAGTAA AACTTGCCCA
    GAAAATCGAT ACGGGAAGGA CTGCGCCCTG TTCTGTGCAT GCGGTAAAGG TCATTGTGAC
    CCACGGACTG GCAGCTGTAC CTGTCCTCCT GGCCAAATGG GACCTGGCTG TCAGCAAGTG
    TGTCCCCAGG GACGGTTCGG CCCCAGCTGC CGCCTGACAT GCAGCTGTCA GAACGGTGCT
    ATCTGTGACC ACGTGGATGG CAGCTGCACT TGTGGACTTG GCTGGACAGG AAAATCGTGT
    GAGAAAGCAG AATGTCTCCC TGGCAAGTAC GGCCCCAACT GCAGCTTGGA CTGCTCATGC
    CAGCACAACG GCACCTGCGA CAGGTTCACG GGCTGCTGCC TCTGCCCCGC GGGGTTCTAC
    GGGCGCTCCT GTGAGCACGG GTGCCCCCCC GGGTTTTATG GGCTGAGCTG CCTTCATGCA
    TGTGCCTGTA AAAACGGAGC GAGCTGCGAT GCCGTGATGG GACAGTGCAT TTGTCCCTCG
    GGGTACCATG GTATCCACTG TGAGAAAGGC TGTGCCAGTG GGCGCTTCGG TGAGCGGTGC
    CAGCGCCAGT GCGACTGTGG AGGAGCTCCC TGCGACCCGG CCACTGGGAA ATGCCTCTGC
    CCACCTGGAA AGACTGGGGA CAAGTGTGAC ATTGGCTGCC GGTCTGACCA GTACGGCCCC
    GACTGCTCTC TGCGATGCCA GTGTGCCAGC AAGTCCCAGT GCAACCCATA CAATGGGAAA
    TGTGTCTGTC CAGCCACGTG GATGGGGCCA ACCTGCAAGG AAGGTGGCCC TCCAAAGCTT
    CCTTTTTATG AAGAACAAAC TCAAGAAAGA GGGAATATTT TTCCGAGAGC TCTACAACAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009349.1
    CDS265..4647
    Translation

    Target ORF information:

    RefSeq Version XM_025153581.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153581.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCTGAGC CTGCGGTGCG ATGTGTTCCC GGGCAGTATC AGGCCAATAC ACACAAACGG 
    CCTTCAGCAG ACTTCTGGCC AAATGTCTGC CCTGTGTTGG AGCTGGTGCT GGTGGGACAC
    CAACAACCAT GTGTTCAGGC CTTCAGCCGC ATGGTGAAGA TGTGGAAACA AGGCTGCACA
    GGGAGGAGAT GGTGCATGGG ATATGAGAGG AGGTCTGGTT ACTACACTGT GTATAAGCAG
    GTGTACAGAA TGGAGCACCA GACTGTCTAT AAGTGCTGCC CTGGGTGGGC TCAACAGAAC
    AGCGAGCTTG GCTGCTTGCA CATGCTCTGT GCTGCTGACA CTTGCTTCAA TGGAGGGAAG
    TGTTCTGAAG AAGGACCTCA CATGTGCCAG TGTCCTGCGG GATTTCAGGG TCCTCGCTGC
    CAGTATGATG TAAATGAGTG CAGCACTGAG AACGGAGGCT GTCAGGACCA GTGCTGTAAC
    ACCATCGGCA GCTACCACTG CAAATGTCCA GCTGGCCAGA AACTGGGGGA AGATGGAAAA
    TCGTGCGGAG ATATAGATGA GTGTGCAGAG GTGAATGGAG GCTGCCAGCA GGGCTGCATC
    AACACCCACA GCTCTTTCCA CTGCCACTGT TGGGCAGGAT ACAGGCTGCA CTCTGATGGG
    CGCACGTGCT TGATGATAGA CCCCTGCACA AGTGGGAATG GAGGATGCTC ACAAATCTGT
    CAAAATGAGA GAGGAACTGC CAAATGTGAC TGTCACCCAG GGCATTATCT TTCTACAGAT
    GAGAAGTCCT GCTTAGACAT TGATGAGTGT GCAGAAGGGA AAGCCAGGTG TGCTCATCAC
    TGTGTGAACA TGCTGGGATC CTTCACCTGT GCCTGTAATC CCGGCTTCGA GCTGGGGGCT
    GATGGGAAGC AGTGCTACCG CATTGAAATG GAGATAGTGG ACAGCTGCCA GCAGGACAAC
    GGTGGGTGCT CCCACCGCTG CGAGCACACA GCAGCAGGAC CACGCTGCTC CTGCCACGAT
    GGCTACGTGC TCAGCTCAGA TGGCAAGGCA TGCACAGACC TGGATGAGTG CGAGACCGGC
    GAGTCGTGCT GCTCCCAGTT CTGCATCAAC TACGTGGGCA GCTACGAGTG CACCTGTAGA
    GCAGGCTTCC AGCCCAGTGC CGATGGCTGC AGCTGCGACG ATGTGGATGA ATGTCGTCTT
    GAGAATGGCA ACTGTGACCA TATCTGCATT AATTCCCTGG GGTCATACGA ATGCGCCTGC
    AAGGAGAGGT ACCGGCTGGG TGCTGACCGG CGCTCCTGCA TTGTGTTAGG AGGGGACTCA
    GCAGTGTCGG GCTTCGCAGG GGCTCCGGGG CTGCAGTTCA GAGGTGCCCC CCAGCTCCTG
    CACTATGTGC TTGGGGCCCC ACGTGAGGAT GAGGAGCAGC GAGGAGAGCT GACACTGATA
    CACAGAGCCG TTTGCCTTGA TAACACTTTT GGCAATGACT GCAGCTTGAG CTGTGAGGAT
    TGTATGAATG GAGGCAGATG TAACAGTGAG AACAGTGGCT GTGTTTGCTC ACCTGGGTGG
    ACCGGCATCA TCTGCAACCA AACCTGCGCC CAGGGTACAT ACGGTGATGG TTGTGCCAGT
    ATCTGCATGT GCCAGAATGG AGGCTCCTGT GACCCCATCA CAGGGCTTTG TCACTGCCCA
    CCAGGAGTCC AGGGGCCATT TTGTGAAGAC GGTTGTCCCA GGGGATTTTT TGGGAAGAAC
    TGCAACAAAC CATGCAACTG TGCCAACAGC GGCCACTGCC ACCGGCTATA TGGAGCCTGC
    CTGTGTGACC CTGGGCTGTA TGGACGCTTC TGCCACCTCA CCTGTCCCAG GTGGGCTTTT
    GGTGTGGGCT GCTCACAGGA GTGTCAGTGT GTGAAGGAGC ACACACTGGG ATGCAGCGCT
    CGGAACGGGA CCTGCAGCTG CAAGCCTGGA TATCACGGCA AGAAGTGCCA GAAAGCATGC
    ACACCTGGCT TTTATGGGGC TGGTTGCAAA CTGAGGTGCA ACTGTTCCAC CGACGTTTTG
    TGTGACCACG AGACGGGAGC CTGCAAGCGT CCGTGCCCGC CTGGTTTCCA TGGAGAAAAG
    TGCCATTCCT CTTGCCAGCC CGGGAGCTTT GGGGTGGACT GCAGGCAGAG CTGCAGCTGC
    GGGGGAGCAC CGTGTGACCC AGAGACTGGG CGTTGTTTCT GCCCAGCTGG GAAAACTGGT
    GCCATGTGTG AGAGAGCCTG CCCTGTTGGA TACTACGGAC AAAACTGCCA GCTGCCATGC
    AGCTGCAGCA GTGATGCTCT GTGTCACCAC ATCACTGGGG AGTGCACGTG TCCCCCCGGC
    TGGACTGGCC ACGACTGCAA GCACCCCTGC AATAGCGGCC GTTGGGGACA GCACTGTGAA
    AACTCCTGTG CCTGCAATAA CAGCGATGGG AGCTGCGATC CTGTCACTGG GTCCTGCTTC
    TGTGAGCCAG GATTCACTGG GAAACACTGC GAGCAGAGAT GTCCTGAGGG AAGCTTTGGT
    CCCAGCTGCA TGCACAGCTG CCAGTGCCAG AACGGGGCTG CATGTGACCA TGTGAGCGGA
    GCCTGCACGT GCAGTGCTGG ATGGACCGGG ACCTTCTGCA GGAAAGCTTG TCCAGAAGGA
    TTTTTTGGGC TTGACTGCCA CCAGGTGTGC AACTGCAAGA ACGCTGAGGG CTGTGACCAT
    GTCCTGGGGA CGTGCCGCTG CCTCCCAGGC TGGAGGGGAG AGACCTGCGA GCAGCCGTGT
    CAGGGGGACA GGTTTGGGCA GGGCTGTGCT CACACCTGCA ACTGTCACAA CGGAGCACTC
    TGCGACCACA TAACGGGGAC ATGTGCCTGC ACCCCAGGCT GGGGAGGAGC CACCTGCCAA
    GAAGCCTGTC CCCCAGGCTG GCATGGTGCT GGCTGCCTTC AGCGCTGCAT TTGCCCTGGC
    CAGACTGCAT GTGACCCCGT GACGGGCAAG TGCCAGTGTT CCAAGGGCGG AACAGGGACA
    TCCTGTGAGC TTGCATGTCC CCCAGGATTT TTTGGAGAGA GATGTGAAGA GCAATGTGAC
    TGCATTCACA GCGTGTCCTG CCACCCCCAA ACGGGGGCTT GTCGCTGTGC CAAGGGGTGG
    CGAGGCAGGC ACTGTGACAA ACCCTGCTTG CCTGGCCACT ACGGCATGGG TTGTGCCCGG
    CGGTGCCACT GCCCTGCAGG CACCCCCTGC CACCACCTGA CGGGTGAGTG CAGCTGTCCT
    CCAGGATTCA CTGGCCATGG CTGCGAGAAA ACTTGTCCAC CAAACACGTA TGGCCAGAGT
    TGCAGCCAAG CCTGTCGCTG CCGTGCTGAG GAGGACTGTC ACCCCATCAC TGGGCGGTGC
    GTGTGCCGCC TGGGGTACCA CGGGGCCCAG TGTGACCGCC CAGGGTGTCC AGAAGGTACT
    TACGGTTCAA ACTGCCAAAA ATCCTGTAAA TGCATGAATG GAGGCCATTG CGACCCAGTG
    TCGGGAACTT GTGATTGCGC GCCTGGTTTC ATTGGAGCCG ACTGTAGTAA AACTTGCCCA
    GAAAATCGAT ACGGGAAGGA CTGCGCCCTG TTCTGTGCAT GCGGTAAAGG TCATTGTGAC
    CCACGGACTG GCAGCTGTAC CTGTCCTCCT GGCCAAATGG GACCTGGCTG TCAGCAAGTG
    TGTCCCCAGG GACGGTTCGG CCCCAGCTGC CGCCTGACAT GCAGCTGTCA GAACGGTGCT
    ATCTGTGACC ACGTGGATGG CAGCTGCACT TGTGGACTTG GCTGGACAGG AAAATCGTGT
    GAGAAAGCAG AATGTCTCCC TGGCAAGTAC GGCCCCAACT GCAGCTTGGA CTGCTCATGC
    CAGCACAACG GCACCTGCGA CAGGTTCACG GGCTGCTGCC TCTGCCCCGC GGGGTTCTAC
    GGGCGCTCCT GTGAGCACGG GTGCCCCCCC GGGTTTTATG GGCTGAGCTG CCTTCATGCA
    TGTGCCTGTA AAAACGGAGC GAGCTGCGAT GCCGTGATGG GACAGTGCAT TTGTCCCTCG
    GGGTACCATG GTATCCACTG TGAGAAAGGC TGTGCCAGTG GGCGCTTCGG TGAGCGGTGC
    CAGCGCCAGT GCGACTGTGG AGGAGCTCCC TGCGACCCGG CCACTGGGAA ATGCCTCTGC
    CCACCTGGAA AGACTGGGGA CAAGTGTGAC ATTGGCTGCC GGTCTGACCA GTACGGCCCC
    GACTGCTCTC TGCGATGCCA GTGTGCCAGC AAGTCCCAGT GCAACCCATA CAATGGGAAA
    TGTGTCTGTC CAGCCACGTG GATGGGGCCA ACCTGCAAGG AAGGTGGCCC TCCAAAGCTT
    CCTTTTTATG AAGAACAAAC TCAAGAAAGA GGGAATATTT TTCCGAGAGC TCTACAACAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152781
    Clone ID Related Accession (Same CDS sequence) XM_025153582.1
    Accession Version XM_025153582.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4233bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGTGAAGA TGTGGAAACA AGGCTGCACA GGGAGGAGAT GGTGCATGGG ATATGAGAGG 
    AGGTCTGGTT ACTACACTGT GTATAAGCAG GTGTACAGAA TGGAGCACCA GACTGTCTAT
    AAGTGCTGCC CTGGGTGGGC TCAACAGAAC AGCGAGCTTG GCTGCTTGCA CATGCTCTGT
    GCTGCTGACA CTTGCTTCAA TGGAGGGAAG TGTTCTGAAG AAGGACCTCA CATGTGCCAG
    TGTCCTGCGG GATTTCAGGG TCCTCGCTGC CAGTATGATG TAAATGAGTG CAGCACTGAG
    AACGGAGGCT GTCAGGACCA GTGCTGTAAC ACCATCGGCA GCTACCACTG CAAATGTCCA
    GCTGGCCAGA AACTGGGGGA AGATGGAAAA TCGTGCGGAG ATATAGATGA GTGTGCAGAG
    GTGAATGGAG GCTGCCAGCA GGGCTGCATC AACACCCACA GCTCTTTCCA CTGCCACTGT
    TGGGCAGGAT ACAGGCTGCA CTCTGATGGG CGCACGTGCT TGATGATAGA CCCCTGCACA
    AGTGGGAATG GAGGATGCTC ACAAATCTGT CAAAATGAGA GAGGAACTGC CAAATGTGAC
    TGTCACCCAG GGCATTATCT TTCTACAGAT GAGAAGTCCT GCTTAGACAT TGATGAGTGT
    GCAGAAGGGA AAGCCAGGTG TGCTCATCAC TGTGTGAACA TGCTGGGATC CTTCACCTGT
    GCCTGTAATC CCGGCTTCGA GCTGGGGGCT GATGGGAAGC AGTGCTACCG CATTGAAATG
    GAGATAGTGG ACAGCTGCCA GCAGGACAAC GGTGGGTGCT CCCACCGCTG CGAGCACACA
    GCAGCAGGAC CACGCTGCTC CTGCCACGAT GGCTACGTGC TCAGCTCAGA TGGCAAGGCA
    TGCACAGACC TGGATGAGTG CGAGACCGGC GAGTCGTGCT GCTCCCAGTT CTGCATCAAC
    TACGTGGGCA GCTACGAGTG CACCTGTAGA GCAGGCTTCC AGCCCAGTGC CGATGGCTGC
    AGCTGCGACG ATGTGGATGA ATGTCGTCTT GAGAATGGCA ACTGTGACCA TATCTGCATT
    AATTCCCTGG GGTCATACGA ATGCGCCTGC AAGGAGAGGT ACCGGCTGGG TGCTGACCGG
    CGCTCCTGCA TTGTGTTAGG AGGGGACTCA GCAGTGTCGG GCTTCGCAGG GGCTCCGGGG
    CTGCAGTTCA GAGGTGCCCC CCAGCTCCTG CACTATGTGC TTGGGGCCCC ACGTGAGGAT
    GAGGAGCAGC GAGGAGAGCT GACACTGATA CACAGAGCCG TTTGCCTTGA TAACACTTTT
    GGCAATGACT GCAGCTTGAG CTGTGAGGAT TGTATGAATG GAGGCAGATG TAACAGTGAG
    AACAGTGGCT GTGTTTGCTC ACCTGGGTGG ACCGGCATCA TCTGCAACCA AACCTGCGCC
    CAGGGTACAT ACGGTGATGG TTGTGCCAGT ATCTGCATGT GCCAGAATGG AGGCTCCTGT
    GACCCCATCA CAGGGCTTTG TCACTGCCCA CCAGGAGTCC AGGGGCCATT TTGTGAAGAC
    GGTTGTCCCA GGGGATTTTT TGGGAAGAAC TGCAACAAAC CATGCAACTG TGCCAACAGC
    GGCCACTGCC ACCGGCTATA TGGAGCCTGC CTGTGTGACC CTGGGCTGTA TGGACGCTTC
    TGCCACCTCA CCTGTCCCAG GTGGGCTTTT GGTGTGGGCT GCTCACAGGA GTGTCAGTGT
    GTGAAGGAGC ACACACTGGG ATGCAGCGCT CGGAACGGGA CCTGCAGCTG CAAGCCTGGA
    TATCACGGCA AGAAGTGCCA GAAAGCATGC ACACCTGGCT TTTATGGGGC TGGTTGCAAA
    CTGAGGTGCA ACTGTTCCAC CGACGTTTTG TGTGACCACG AGACGGGAGC CTGCAAGCGT
    CCGTGCCCGC CTGGTTTCCA TGGAGAAAAG TGCCATTCCT CTTGCCAGCC CGGGAGCTTT
    GGGGTGGACT GCAGGCAGAG CTGCAGCTGC GGGGGAGCAC CGTGTGACCC AGAGACTGGG
    CGTTGTTTCT GCCCAGCTGG GAAAACTGGT GCCATGTGTG AGAGAGCCTG CCCTGTTGGA
    TACTACGGAC AAAACTGCCA GCTGCCATGC AGCTGCAGCA GTGATGCTCT GTGTCACCAC
    ATCACTGGGG AGTGCACGTG TCCCCCCGGC TGGACTGGCC ACGACTGCAA GCACCCCTGC
    AATAGCGGCC GTTGGGGACA GCACTGTGAA AACTCCTGTG CCTGCAATAA CAGCGATGGG
    AGCTGCGATC CTGTCACTGG GTCCTGCTTC TGTGAGCCAG GATTCACTGG GAAACACTGC
    GAGCAGAGAT GTCCTGAGGG AAGCTTTGGT CCCAGCTGCA TGCACAGCTG CCAGTGCCAG
    AACGGGGCTG CATGTGACCA TGTGAGCGGA GCCTGCACGT GCAGTGCTGG ATGGACCGGG
    ACCTTCTGCA GGAAAGCTTG TCCAGAAGGA TTTTTTGGGC TTGACTGCCA CCAGGTGTGC
    AACTGCAAGA ACGCTGAGGG CTGTGACCAT GTCCTGGGGA CGTGCCGCTG CCTCCCAGGC
    TGGAGGGGAG AGACCTGCGA GCAGCCGTGT CAGGGGGACA GGTTTGGGCA GGGCTGTGCT
    CACACCTGCA ACTGTCACAA CGGAGCACTC TGCGACCACA TAACGGGGAC ATGTGCCTGC
    ACCCCAGGCT GGGGAGGAGC CACCTGCCAA GAAGCCTGTC CCCCAGGCTG GCATGGTGCT
    GGCTGCCTTC AGCGCTGCAT TTGCCCTGGC CAGACTGCAT GTGACCCCGT GACGGGCAAG
    TGCCAGTGTT CCAAGGGCGG AACAGGGACA TCCTGTGAGC TTGCATGTCC CCCAGGATTT
    TTTGGAGAGA GATGTGAAGA GCAATGTGAC TGCATTCACA GCGTGTCCTG CCACCCCCAA
    ACGGGGGCTT GTCGCTGTGC CAAGGGGTGG CGAGGCAGGC ACTGTGACAA ACCCTGCTTG
    CCTGGCCACT ACGGCATGGG TTGTGCCCGG CGGTGCCACT GCCCTGCAGG CACCCCCTGC
    CACCACCTGA CGGGTGAGTG CAGCTGTCCT CCAGGATTCA CTGGCCATGG CTGCGAGAAA
    ACTTGTCCAC CAAACACGTA TGGCCAGAGT TGCAGCCAAG CCTGTCGCTG CCGTGCTGAG
    GAGGACTGTC ACCCCATCAC TGGGCGGTGC GTGTGCCGCC TGGGGTACCA CGGGGCCCAG
    TGTGACCGCC CAGGGTGTCC AGAAGGTACT TACGGTTCAA ACTGCCAAAA ATCCTGTAAA
    TGCATGAATG GAGGCCATTG CGACCCAGTG TCGGGAACTT GTGATTGCGC GCCTGGTTTC
    ATTGGAGCCG ACTGTAGTAA AACTTGCCCA GAAAATCGAT ACGGGAAGGA CTGCGCCCTG
    TTCTGTGCAT GCGGTAAAGG TCATTGTGAC CCACGGACTG GCAGCTGTAC CTGTCCTCCT
    GGCCAAATGG GACCTGGCTG TCAGCAAGTG TGTCCCCAGG GACGGTTCGG CCCCAGCTGC
    CGCCTGACAT GCAGCTGTCA GAACGGTGCT ATCTGTGACC ACGTGGATGG CAGCTGCACT
    TGTGGACTTG GCTGGACAGG AAAATCGTGT GAGAAAGCAG AATGTCTCCC TGGCAAGTAC
    GGCCCCAACT GCAGCTTGGA CTGCTCATGC CAGCACAACG GCACCTGCGA CAGGTTCACG
    GGCTGCTGCC TCTGCCCCGC GGGGTTCTAC GGGCGCTCCT GTGAGCACGG GTGCCCCCCC
    GGGTTTTATG GGCTGAGCTG CCTTCATGCA TGTGCCTGTA AAAACGGAGC GAGCTGCGAT
    GCCGTGATGG GACAGTGCAT TTGTCCCTCG GGGTACCATG GTATCCACTG TGAGAAAGGC
    TGTGCCAGTG GGCGCTTCGG TGAGCGGTGC CAGCGCCAGT GCGACTGTGG AGGAGCTCCC
    TGCGACCCGG CCACTGGGAA ATGCCTCTGC CCACCTGGAA AGACTGGGGA CAAGTGTGAC
    ATTGGCTGCC GGTCTGACCA GTACGGCCCC GACTGCTCTC TGCGATGCCA GTGTGCCAGC
    AAGTCCCAGT GCAACCCATA CAATGGGAAA TGTGTCTGTC CAGCCACGTG GATGGGGCCA
    ACCTGCAAGG AAGGTGGCCC TCCAAAGCTT CCTTTTTATG AAGAACAAAC TCAAGAAAGA
    GGGAATATTT TTCCGAGAGC TCTACAACAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009350.1
    CDS556..4788
    Translation

    Target ORF information:

    RefSeq Version XM_025153582.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153582.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGTGAAGA TGTGGAAACA AGGCTGCACA GGGAGGAGAT GGTGCATGGG ATATGAGAGG 
    AGGTCTGGTT ACTACACTGT GTATAAGCAG GTGTACAGAA TGGAGCACCA GACTGTCTAT
    AAGTGCTGCC CTGGGTGGGC TCAACAGAAC AGCGAGCTTG GCTGCTTGCA CATGCTCTGT
    GCTGCTGACA CTTGCTTCAA TGGAGGGAAG TGTTCTGAAG AAGGACCTCA CATGTGCCAG
    TGTCCTGCGG GATTTCAGGG TCCTCGCTGC CAGTATGATG TAAATGAGTG CAGCACTGAG
    AACGGAGGCT GTCAGGACCA GTGCTGTAAC ACCATCGGCA GCTACCACTG CAAATGTCCA
    GCTGGCCAGA AACTGGGGGA AGATGGAAAA TCGTGCGGAG ATATAGATGA GTGTGCAGAG
    GTGAATGGAG GCTGCCAGCA GGGCTGCATC AACACCCACA GCTCTTTCCA CTGCCACTGT
    TGGGCAGGAT ACAGGCTGCA CTCTGATGGG CGCACGTGCT TGATGATAGA CCCCTGCACA
    AGTGGGAATG GAGGATGCTC ACAAATCTGT CAAAATGAGA GAGGAACTGC CAAATGTGAC
    TGTCACCCAG GGCATTATCT TTCTACAGAT GAGAAGTCCT GCTTAGACAT TGATGAGTGT
    GCAGAAGGGA AAGCCAGGTG TGCTCATCAC TGTGTGAACA TGCTGGGATC CTTCACCTGT
    GCCTGTAATC CCGGCTTCGA GCTGGGGGCT GATGGGAAGC AGTGCTACCG CATTGAAATG
    GAGATAGTGG ACAGCTGCCA GCAGGACAAC GGTGGGTGCT CCCACCGCTG CGAGCACACA
    GCAGCAGGAC CACGCTGCTC CTGCCACGAT GGCTACGTGC TCAGCTCAGA TGGCAAGGCA
    TGCACAGACC TGGATGAGTG CGAGACCGGC GAGTCGTGCT GCTCCCAGTT CTGCATCAAC
    TACGTGGGCA GCTACGAGTG CACCTGTAGA GCAGGCTTCC AGCCCAGTGC CGATGGCTGC
    AGCTGCGACG ATGTGGATGA ATGTCGTCTT GAGAATGGCA ACTGTGACCA TATCTGCATT
    AATTCCCTGG GGTCATACGA ATGCGCCTGC AAGGAGAGGT ACCGGCTGGG TGCTGACCGG
    CGCTCCTGCA TTGTGTTAGG AGGGGACTCA GCAGTGTCGG GCTTCGCAGG GGCTCCGGGG
    CTGCAGTTCA GAGGTGCCCC CCAGCTCCTG CACTATGTGC TTGGGGCCCC ACGTGAGGAT
    GAGGAGCAGC GAGGAGAGCT GACACTGATA CACAGAGCCG TTTGCCTTGA TAACACTTTT
    GGCAATGACT GCAGCTTGAG CTGTGAGGAT TGTATGAATG GAGGCAGATG TAACAGTGAG
    AACAGTGGCT GTGTTTGCTC ACCTGGGTGG ACCGGCATCA TCTGCAACCA AACCTGCGCC
    CAGGGTACAT ACGGTGATGG TTGTGCCAGT ATCTGCATGT GCCAGAATGG AGGCTCCTGT
    GACCCCATCA CAGGGCTTTG TCACTGCCCA CCAGGAGTCC AGGGGCCATT TTGTGAAGAC
    GGTTGTCCCA GGGGATTTTT TGGGAAGAAC TGCAACAAAC CATGCAACTG TGCCAACAGC
    GGCCACTGCC ACCGGCTATA TGGAGCCTGC CTGTGTGACC CTGGGCTGTA TGGACGCTTC
    TGCCACCTCA CCTGTCCCAG GTGGGCTTTT GGTGTGGGCT GCTCACAGGA GTGTCAGTGT
    GTGAAGGAGC ACACACTGGG ATGCAGCGCT CGGAACGGGA CCTGCAGCTG CAAGCCTGGA
    TATCACGGCA AGAAGTGCCA GAAAGCATGC ACACCTGGCT TTTATGGGGC TGGTTGCAAA
    CTGAGGTGCA ACTGTTCCAC CGACGTTTTG TGTGACCACG AGACGGGAGC CTGCAAGCGT
    CCGTGCCCGC CTGGTTTCCA TGGAGAAAAG TGCCATTCCT CTTGCCAGCC CGGGAGCTTT
    GGGGTGGACT GCAGGCAGAG CTGCAGCTGC GGGGGAGCAC CGTGTGACCC AGAGACTGGG
    CGTTGTTTCT GCCCAGCTGG GAAAACTGGT GCCATGTGTG AGAGAGCCTG CCCTGTTGGA
    TACTACGGAC AAAACTGCCA GCTGCCATGC AGCTGCAGCA GTGATGCTCT GTGTCACCAC
    ATCACTGGGG AGTGCACGTG TCCCCCCGGC TGGACTGGCC ACGACTGCAA GCACCCCTGC
    AATAGCGGCC GTTGGGGACA GCACTGTGAA AACTCCTGTG CCTGCAATAA CAGCGATGGG
    AGCTGCGATC CTGTCACTGG GTCCTGCTTC TGTGAGCCAG GATTCACTGG GAAACACTGC
    GAGCAGAGAT GTCCTGAGGG AAGCTTTGGT CCCAGCTGCA TGCACAGCTG CCAGTGCCAG
    AACGGGGCTG CATGTGACCA TGTGAGCGGA GCCTGCACGT GCAGTGCTGG ATGGACCGGG
    ACCTTCTGCA GGAAAGCTTG TCCAGAAGGA TTTTTTGGGC TTGACTGCCA CCAGGTGTGC
    AACTGCAAGA ACGCTGAGGG CTGTGACCAT GTCCTGGGGA CGTGCCGCTG CCTCCCAGGC
    TGGAGGGGAG AGACCTGCGA GCAGCCGTGT CAGGGGGACA GGTTTGGGCA GGGCTGTGCT
    CACACCTGCA ACTGTCACAA CGGAGCACTC TGCGACCACA TAACGGGGAC ATGTGCCTGC
    ACCCCAGGCT GGGGAGGAGC CACCTGCCAA GAAGCCTGTC CCCCAGGCTG GCATGGTGCT
    GGCTGCCTTC AGCGCTGCAT TTGCCCTGGC CAGACTGCAT GTGACCCCGT GACGGGCAAG
    TGCCAGTGTT CCAAGGGCGG AACAGGGACA TCCTGTGAGC TTGCATGTCC CCCAGGATTT
    TTTGGAGAGA GATGTGAAGA GCAATGTGAC TGCATTCACA GCGTGTCCTG CCACCCCCAA
    ACGGGGGCTT GTCGCTGTGC CAAGGGGTGG CGAGGCAGGC ACTGTGACAA ACCCTGCTTG
    CCTGGCCACT ACGGCATGGG TTGTGCCCGG CGGTGCCACT GCCCTGCAGG CACCCCCTGC
    CACCACCTGA CGGGTGAGTG CAGCTGTCCT CCAGGATTCA CTGGCCATGG CTGCGAGAAA
    ACTTGTCCAC CAAACACGTA TGGCCAGAGT TGCAGCCAAG CCTGTCGCTG CCGTGCTGAG
    GAGGACTGTC ACCCCATCAC TGGGCGGTGC GTGTGCCGCC TGGGGTACCA CGGGGCCCAG
    TGTGACCGCC CAGGGTGTCC AGAAGGTACT TACGGTTCAA ACTGCCAAAA ATCCTGTAAA
    TGCATGAATG GAGGCCATTG CGACCCAGTG TCGGGAACTT GTGATTGCGC GCCTGGTTTC
    ATTGGAGCCG ACTGTAGTAA AACTTGCCCA GAAAATCGAT ACGGGAAGGA CTGCGCCCTG
    TTCTGTGCAT GCGGTAAAGG TCATTGTGAC CCACGGACTG GCAGCTGTAC CTGTCCTCCT
    GGCCAAATGG GACCTGGCTG TCAGCAAGTG TGTCCCCAGG GACGGTTCGG CCCCAGCTGC
    CGCCTGACAT GCAGCTGTCA GAACGGTGCT ATCTGTGACC ACGTGGATGG CAGCTGCACT
    TGTGGACTTG GCTGGACAGG AAAATCGTGT GAGAAAGCAG AATGTCTCCC TGGCAAGTAC
    GGCCCCAACT GCAGCTTGGA CTGCTCATGC CAGCACAACG GCACCTGCGA CAGGTTCACG
    GGCTGCTGCC TCTGCCCCGC GGGGTTCTAC GGGCGCTCCT GTGAGCACGG GTGCCCCCCC
    GGGTTTTATG GGCTGAGCTG CCTTCATGCA TGTGCCTGTA AAAACGGAGC GAGCTGCGAT
    GCCGTGATGG GACAGTGCAT TTGTCCCTCG GGGTACCATG GTATCCACTG TGAGAAAGGC
    TGTGCCAGTG GGCGCTTCGG TGAGCGGTGC CAGCGCCAGT GCGACTGTGG AGGAGCTCCC
    TGCGACCCGG CCACTGGGAA ATGCCTCTGC CCACCTGGAA AGACTGGGGA CAAGTGTGAC
    ATTGGCTGCC GGTCTGACCA GTACGGCCCC GACTGCTCTC TGCGATGCCA GTGTGCCAGC
    AAGTCCCAGT GCAACCCATA CAATGGGAAA TGTGTCTGTC CAGCCACGTG GATGGGGCCA
    ACCTGCAAGG AAGGTGGCCC TCCAAAGCTT CCTTTTTATG AAGAACAAAC TCAAGAAAGA
    GGGAATATTT TTCCGAGAGC TCTACAACAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152782
    Clone ID Related Accession (Same CDS sequence) XM_025153584.1
    Accession Version XM_025153584.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3876bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCGGTGTCCA GAAGGTACTT ACGGTTCAAA CTGCCAAAAA
    TCCTGTAAAT GCATGAATGG AGGCCATTGC GACCCAGTGT CGGGAACTTG TGATTGCGCG
    CCTGGTTTCA TTGGAGCCGA CTGTAGTAAA ACTTGCCCAG AAAATCGATA CGGGAAGGAC
    TGCGCCCTGT TCTGTGCATG CGGTAAAGGT CATTGTGACC CACGGACTGG CAGCTGTACC
    TGTCCTCCTG GCCAAATGGG ACCTGGCTGT CAGCAAGGGA CGGTTCGGCC CCAGCTGCCG
    CCTGACATGC AGCTGTCAGA ACGGTGCTAT CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009352.1
    CDS181..4056
    Translation

    Target ORF information:

    RefSeq Version XM_025153584.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153584.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCGGTGTCCA GAAGGTACTT ACGGTTCAAA CTGCCAAAAA
    TCCTGTAAAT GCATGAATGG AGGCCATTGC GACCCAGTGT CGGGAACTTG TGATTGCGCG
    CCTGGTTTCA TTGGAGCCGA CTGTAGTAAA ACTTGCCCAG AAAATCGATA CGGGAAGGAC
    TGCGCCCTGT TCTGTGCATG CGGTAAAGGT CATTGTGACC CACGGACTGG CAGCTGTACC
    TGTCCTCCTG GCCAAATGGG ACCTGGCTGT CAGCAAGGGA CGGTTCGGCC CCAGCTGCCG
    CCTGACATGC AGCTGTCAGA ACGGTGCTAT CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40123
    Clone ID Related Accession (Same CDS sequence) XM_015291745.1
    Accession Version XM_015291745.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4413bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCATG TCCCCCAGGA
    TTTTTTGGAG AGAGATGTGA AGAGCAATGT GACTGCATTC ACAGCGTGTC CTGCCACCCC
    CAAACGGGGG CTTGTCGCTG TGCCAAGGGG TGGCGAGGCA GGCACTGTGA CAAACCCTGC
    TTGCCTGGCC ACTACGGCAT GGGTTGTGCC CGGCGGTGCC ACTGCCCTGC AGGCACCCCC
    TGCCACCACC TGACGGGTGA GTGCAGCTGT CCTCCAGGAT TCACTGGCCA TGGCTGCGAG
    AAAACTTGTC CACCAAACAC GTATGGCCAG AGTTGCAGCC AAGCCTGTCG CTGCCGTGCT
    GAGGAGGACT GTCACCCCAT CACTGGGCGG TGCGTGTGCC GCCTGGGGTA CCACGGGGCC
    CAGTGTGACC GCCCAGGGTG TCCAGAAGGT ACTTACGGTT CAAACTGCCA AAAATCCTGT
    AAATGCATGA ATGGAGGCCA TTGCGACCCA GTGTCGGGAA CTTGTGATTG CGCGCCTGGT
    TTCATTGGAG CCGACTGTAG TAAAACTTGC CCAGAAAATC GATACGGGAA GGACTGCGCC
    CTGTTCTGTG CATGCGGTAA AGGTCATTGT GACCCACGGA CTGGCAGCTG TACCTGTCCT
    CCTGGCCAAA TGGGACCTGG CTGTCAGCAA GTGTGTCCCC AGGGACGGTT CGGCCCCAGC
    TGCCGCCTGA CATGCAGCTG TCAGAACGGT GCTATCTGTG ACCACGTGGA TGGCAGCTGC
    ACTTGTGGAC TTGGCTGGAC AGGAAAATCG TGTGAGAAAG AATGTCTCCC TGGCAAGTAC
    GGCCCCAACT GCAGCTTGGA CTGCTCATGC CAGCACAACG GCACCTGCGA CAGGTTCACG
    GGCTGCTGCC TCTGCCCCGC GGGGTTCTAC GGGCGCTCCT GTGAGCACGG GTGCCCCCCC
    GGGTTTTATG GGCTGAGCTG CCTTCATGCA TGTGCCTGTA AAAACGGAGC GAGCTGCGAT
    GCCGTGATGG GACAGTGCAT TTGTCCCTCG GGGTACCATG GTATCCACTG TGAGAAAGGC
    TGTGCCAGTG GGCGCTTCGG TGAGCGGTGC CAGCGCCAGT GCGACTGTGG AGGAGCTCCC
    TGCGACCCGG CCACTGGGAA ATGCCTCTGC CCACCTGGAA AGACTGGGGA CAAGTGTGAC
    ATTGGCTGCC GGTCTGACCA GTACGGCCCC GACTGCTCTC TGCGATGCCA GTGTGCCAGC
    AAGTCCCAGT GCAACCCATA CAATGGGAAA TGTGTCTGTC CAGCCACGTG GATGGGGCCA
    ACCTGCAAGG AAGGTGGCCC TCCAAAGCTT CCTTTTTATG AAGAACAAAC TCAAGAAAGA
    GGGAATATTT TTCCGAGAGC TCTACAACAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147231.1
    CDS176..4588
    Translation

    Target ORF information:

    RefSeq Version XM_015291745.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291745.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCATG TCCCCCAGGA
    TTTTTTGGAG AGAGATGTGA AGAGCAATGT GACTGCATTC ACAGCGTGTC CTGCCACCCC
    CAAACGGGGG CTTGTCGCTG TGCCAAGGGG TGGCGAGGCA GGCACTGTGA CAAACCCTGC
    TTGCCTGGCC ACTACGGCAT GGGTTGTGCC CGGCGGTGCC ACTGCCCTGC AGGCACCCCC
    TGCCACCACC TGACGGGTGA GTGCAGCTGT CCTCCAGGAT TCACTGGCCA TGGCTGCGAG
    AAAACTTGTC CACCAAACAC GTATGGCCAG AGTTGCAGCC AAGCCTGTCG CTGCCGTGCT
    GAGGAGGACT GTCACCCCAT CACTGGGCGG TGCGTGTGCC GCCTGGGGTA CCACGGGGCC
    CAGTGTGACC GCCCAGGGTG TCCAGAAGGT ACTTACGGTT CAAACTGCCA AAAATCCTGT
    AAATGCATGA ATGGAGGCCA TTGCGACCCA GTGTCGGGAA CTTGTGATTG CGCGCCTGGT
    TTCATTGGAG CCGACTGTAG TAAAACTTGC CCAGAAAATC GATACGGGAA GGACTGCGCC
    CTGTTCTGTG CATGCGGTAA AGGTCATTGT GACCCACGGA CTGGCAGCTG TACCTGTCCT
    CCTGGCCAAA TGGGACCTGG CTGTCAGCAA GTGTGTCCCC AGGGACGGTT CGGCCCCAGC
    TGCCGCCTGA CATGCAGCTG TCAGAACGGT GCTATCTGTG ACCACGTGGA TGGCAGCTGC
    ACTTGTGGAC TTGGCTGGAC AGGAAAATCG TGTGAGAAAG AATGTCTCCC TGGCAAGTAC
    GGCCCCAACT GCAGCTTGGA CTGCTCATGC CAGCACAACG GCACCTGCGA CAGGTTCACG
    GGCTGCTGCC TCTGCCCCGC GGGGTTCTAC GGGCGCTCCT GTGAGCACGG GTGCCCCCCC
    GGGTTTTATG GGCTGAGCTG CCTTCATGCA TGTGCCTGTA AAAACGGAGC GAGCTGCGAT
    GCCGTGATGG GACAGTGCAT TTGTCCCTCG GGGTACCATG GTATCCACTG TGAGAAAGGC
    TGTGCCAGTG GGCGCTTCGG TGAGCGGTGC CAGCGCCAGT GCGACTGTGG AGGAGCTCCC
    TGCGACCCGG CCACTGGGAA ATGCCTCTGC CCACCTGGAA AGACTGGGGA CAAGTGTGAC
    ATTGGCTGCC GGTCTGACCA GTACGGCCCC GACTGCTCTC TGCGATGCCA GTGTGCCAGC
    AAGTCCCAGT GCAACCCATA CAATGGGAAA TGTGTCTGTC CAGCCACGTG GATGGGGCCA
    ACCTGCAAGG AAGGTGGCCC TCCAAAGCTT CCTTTTTATG AAGAACAAAC TCAAGAAAGA
    GGGAATATTT TTCCGAGAGC TCTACAACAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40124
    Clone ID Related Accession (Same CDS sequence) XM_015291746.1
    Accession Version XM_015291746.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGGAGCGG TGTGGCTGTG CCTCGGGCTG GGCTGGGCGC TGGCGGCCCG CGTGAGCAGA 
    GCGGAGGAGC CGAGACTGCA GAGGGGCATG CCAAATGTCT GCCCTGTGTT GGAGCTGGTG
    CTGGTGGGAC ACCAACAACC ATGTGTTCAG GCCTTCAGCC GCATGGTGAA GATGTGGAAA
    CAAGGCTGCA CAGGGAGGAG ATGGTGCATG GGATATGAGA GGAGGTCTGG TTACTACACT
    GTGTATAAGC AGGTGTACAG AATGGAGCAC CAGACTGTCT ATAAGTGCTG CCCTGGGTGG
    GCTCAACAGA ACAGCGAGCT TGGCTGCTTG CACATGCTCT GTGCTGCTGA CACTTGCTTC
    AATGGAGGGA AGTGTTCTGA AGAAGGACCT CACATGTGCC AGTGTCCTGC GGGATTTCAG
    GGTCCTCGCT GCCAGTATGA TGTAAATGAG TGCAGCACTG AGAACGGAGG CTGTCAGGAC
    CAGTGCTGTA ACACCATCGG CAGCTACCAC TGCAAATGTC CAGCTGGCCA GAAACTGGGG
    GAAGATGGAA AATCGTGCGG AGATATAGAT GAGTGTGCAG AGGTGAATGG AGGCTGCCAG
    CAGGGCTGCA TCAACACCCA CAGCTCTTTC CACTGCCACT GTTGGGCAGG ATACAGGCTG
    CACTCTGATG GGCGCACGTG CTTGATGATA GACCCCTGCA CAAGTGGGAA TGGAGGATGC
    TCACAAATCT GTCAAAATGA GAGAGGAACT GCCAAATGTG ACTGTCACCC AGGGCATTAT
    CTTTCTACAG ATGAGAAGTC CTGCTTAGAC ATTGATGAGT GTGCAGAAGG GAAAGCCAGG
    TGTGCTCATC ACTGTGTGAA CATGCTGGGA TCCTTCACCT GTGCCTGTAA TCCCGGCTTC
    GAGCTGGGGG CTGATGGGAA GCAGTGCTAC CGCATTGAAA TGGAGATAGT GGACAGCTGC
    CAGCAGGACA ACGGTGGGTG CTCCCACCGC TGCGAGCACA CAGCAGCAGG ACCACGCTGC
    TCCTGCCACG ATGGCTACGT GCTCAGCTCA GATGGCAAGG CATGCACAGA CCTGGATGAG
    TGCGAGACCG GCGAGTCGTG CTGCTCCCAG TTCTGCATCA ACTACGTGGG CAGCTACGAG
    TGCACCTGTA GAGCAGGCTT CCAGCCCAGT GCCGATGGCT GCAGCTGCGA CGATGTGGAT
    GAATGTCGTC TTGAGAATGG CAACTGTGAC CATATCTGCA TTAATTCCCT GGGGTCATAC
    GAATGCGCCT GCAAGGAGAG GTACCGGCTG GGTGCTGACC GGCGCTCCTG CATTGTGTTA
    GGAGGGGACT CAGCAGTGTC GGGCTTCGCA GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC
    CCCCAGCTCC TGCACTATGT GCTTGGGGCC CCACGTGAGG ATGAGGAGCA GCGAGGAGAG
    CTGACACTGA TACACAGAGC CGTTTGCCTT GATAACACTT TTGGCAATGA CTGCAGCTTG
    AGCTGTGAGG ATTGTATGAA TGGAGGCAGA TGTAACAGTG AGAACAGTGG CTGTGTTTGC
    TCACCTGGGT GGACCGGCAT CATCTGCAAC CAAACCTGCG CCCAGGGTAC ATACGGTGAT
    GGTTGTGCCA GTATCTGCAT GTGCCAGAAT GGAGGCTCCT GTGACCCCAT CACAGGGCTT
    TGTCACTGCC CACCAGGAGT CCAGGGGCCA TTTTGTGAAG ACGGTTGTCC CAGGGGATTT
    TTTGGGAAGA ACTGCAACAA ACCATGCAAC TGTGCCAACA GCGGCCACTG CCACCGGCTA
    TATGGAGCCT GCCTGTGTGA CCCTGGGCTG TATGGACGCT TCTGCCACCT CACCTGTCCC
    AGGTGGGCTT TTGGTGTGGG CTGCTCACAG GAGTGTCAGT GTGTGAAGGA GCACACACTG
    GGATGCAGCG CTCGGAACGG GACCTGCAGC TGCAAGCCTG GATATCACGG CAAGAAGTGC
    CAGAAAGCAT GCACACCTGG CTTTTATGGG GCTGGTTGCA AACTGAGGTG CAACTGTTCC
    ACCGACGTTT TGTGTGACCA CGAGACGGGA GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC
    CATGGAGAAA AGTGCCATTC CTCTTGCCAG CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG
    AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC CCAGAGACTG GGCGTTGTTT CTGCCCAGCT
    GGGAAAACTG GTGCCATGTG TGAGAGAGCC TGCCCTGTTG GATACTACGG ACAAAACTGC
    CAGCTGCCAT GCAGCTGCAG CAGTGATGCT CTGTGTCACC ACATCACTGG GGAGTGCACG
    TGTCCCCCCG GCTGGACTGG CCACGACTGC AAGCACCCCT GCAATAGCGG CCGTTGGGGA
    CAGCACTGTG AAAACTCCTG TGCCTGCAAT AACAGCGATG GGAGCTGCGA TCCTGTCACT
    GGGTCCTGCT TCTGTGAGCC AGGATTCACT GGGAAACACT GCGAGCAGAG ATGTCCTGAG
    GGAAGCTTTG GTCCCAGCTG CATGCACAGC TGCCAGTGCC AGAACGGGGC TGCATGTGAC
    CATGTGAGCG GAGCCTGCAC GTGCAGTGCT GGATGGACCG GGACCTTCTG CAGGAAAGCT
    TGTCCAGAAG GATTTTTTGG GCTTGACTGC CACCAGGTGT GCAACTGCAA GAACGCTGAG
    GGCTGTGACC ATGTCCTGGG GACGTGCCGC TGCCTCCCAG GCTGGAGGGG AGAGACCTGC
    GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG CAGGGCTGTG CTCACACCTG CAACTGTCAC
    AACGGAGCAC TCTGCGACCA CATAACGGGG ACATGTGCCT GCACCCCAGG CTGGGGAGGA
    GCCACCTGCC AAGAAGCCTG TCCCCCAGGC TGGCATGGTG CTGGCTGCCT TCAGCGCTGC
    ATTTGCCCTG GCCAGACTGC ATGTGACCCC GTGACGGGCA AGTGCCAGTG TTCCAAGGGC
    GGAACAGGGA CATCCTGTGA GCTTGCATGT CCCCCAGGAT TTTTTGGAGA GAGATGTGAA
    GAGCAATGTG ACTGCATTCA CAGCGTGTCC TGCCACCCCC AAACGGGGGC TTGTCGCTGT
    GCCAAGGGGT GGCGAGGCAG GCACTGTGAC AAACCCTGCT TGCCTGGCCA CTACGGCATG
    GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA GGCACCCCCT GCCACCACCT GACGGGTGAG
    TGCAGCTGTC CTCCAGGATT CACTGGCCAT GGCTGCGAGA AAACTTGTCC ACCAAACACG
    TATGGCCAGA GTTGCAGCCA AGCCTGTCGC TGCCGTGCTG AGGAGGACTG TCACCCCATC
    ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC CACGGGGCCC AGTGTGACCG CCCAGGGTGT
    CCAGAAGGTA CTTACGGTTC AAACTGCCAA AAATCCTGTA AATGCATGAA TGGAGGCCAT
    TGCGACCCAG TGTCGGGAAC TTGTGATTGC GCGCCTGGTT TCATTGGAGC CGACTGTAGT
    AAAACTTGCC CAGAAAATCG ATACGGGAAG GACTGCGCCC TGTTCTGTGC ATGCGGTAAA
    GGTCATTGTG ACCCACGGAC TGGCAGCTGT ACCTGTCCTC CTGGCCAAAT GGGACCTGGC
    TGTCAGCAAG TGTGTCCCCA GGGACGGTTC GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT
    CAGAACGGTG CTATCTGTGA CCACGTGGAT GGCAGCTGCA CTTGTGGACT TGGCTGGACA
    GGAAAATCGT GTGAGAAAGA ATGTCTCCCT GGCAAGTACG GCCCCAACTG CAGCTTGGAC
    TGCTCATGCC AGCACAACGG CACCTGCGAC AGGTTCACGG GCTGCTGCCT CTGCCCCGCG
    GGGTTCTACG GGCGCTCCTG TGAGCACGGG TGCCCCCCCG GGTTTTATGG GCTGAGCTGC
    CTTCATGCAT GTGCCTGTAA AAACGGAGCG AGCTGCGATG CCGTGATGGG ACAGTGCATT
    TGTCCCTCGG GGTACCATGG TATCCACTGT GAGAAAGGCT GTGCCAGTGG GCGCTTCGGT
    GAGCGGTGCC AGCGCCAGTG CGACTGTGGA GGAGCTCCCT GCGACCCGGC CACTGGGAAA
    TGCCTCTGCC CACCTGGAAA GACTGGGGAC AAGTGTGACA TTGGCTGCCG GTCTGACCAG
    TACGGCCCCG ACTGCTCTCT GCGATGCCAG TGTGCCAGCA AGTCCCAGTG CAACCCATAC
    AATGGGAAAT GTGTCTGTCC AGCCACGTGG ATGGGGCCAA CCTGCAAGGA AGGTGGCCCT
    CCAAAGCTTC CTTTTTATGA AGAACAAACT CAAGAAAGAG GGAATATTTT TCCGAGAGCT
    CTACAACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147232.1
    CDS176..4567
    Translation

    Target ORF information:

    RefSeq Version XM_015291746.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291746.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGGAGCGG TGTGGCTGTG CCTCGGGCTG GGCTGGGCGC TGGCGGCCCG CGTGAGCAGA 
    GCGGAGGAGC CGAGACTGCA GAGGGGCATG CCAAATGTCT GCCCTGTGTT GGAGCTGGTG
    CTGGTGGGAC ACCAACAACC ATGTGTTCAG GCCTTCAGCC GCATGGTGAA GATGTGGAAA
    CAAGGCTGCA CAGGGAGGAG ATGGTGCATG GGATATGAGA GGAGGTCTGG TTACTACACT
    GTGTATAAGC AGGTGTACAG AATGGAGCAC CAGACTGTCT ATAAGTGCTG CCCTGGGTGG
    GCTCAACAGA ACAGCGAGCT TGGCTGCTTG CACATGCTCT GTGCTGCTGA CACTTGCTTC
    AATGGAGGGA AGTGTTCTGA AGAAGGACCT CACATGTGCC AGTGTCCTGC GGGATTTCAG
    GGTCCTCGCT GCCAGTATGA TGTAAATGAG TGCAGCACTG AGAACGGAGG CTGTCAGGAC
    CAGTGCTGTA ACACCATCGG CAGCTACCAC TGCAAATGTC CAGCTGGCCA GAAACTGGGG
    GAAGATGGAA AATCGTGCGG AGATATAGAT GAGTGTGCAG AGGTGAATGG AGGCTGCCAG
    CAGGGCTGCA TCAACACCCA CAGCTCTTTC CACTGCCACT GTTGGGCAGG ATACAGGCTG
    CACTCTGATG GGCGCACGTG CTTGATGATA GACCCCTGCA CAAGTGGGAA TGGAGGATGC
    TCACAAATCT GTCAAAATGA GAGAGGAACT GCCAAATGTG ACTGTCACCC AGGGCATTAT
    CTTTCTACAG ATGAGAAGTC CTGCTTAGAC ATTGATGAGT GTGCAGAAGG GAAAGCCAGG
    TGTGCTCATC ACTGTGTGAA CATGCTGGGA TCCTTCACCT GTGCCTGTAA TCCCGGCTTC
    GAGCTGGGGG CTGATGGGAA GCAGTGCTAC CGCATTGAAA TGGAGATAGT GGACAGCTGC
    CAGCAGGACA ACGGTGGGTG CTCCCACCGC TGCGAGCACA CAGCAGCAGG ACCACGCTGC
    TCCTGCCACG ATGGCTACGT GCTCAGCTCA GATGGCAAGG CATGCACAGA CCTGGATGAG
    TGCGAGACCG GCGAGTCGTG CTGCTCCCAG TTCTGCATCA ACTACGTGGG CAGCTACGAG
    TGCACCTGTA GAGCAGGCTT CCAGCCCAGT GCCGATGGCT GCAGCTGCGA CGATGTGGAT
    GAATGTCGTC TTGAGAATGG CAACTGTGAC CATATCTGCA TTAATTCCCT GGGGTCATAC
    GAATGCGCCT GCAAGGAGAG GTACCGGCTG GGTGCTGACC GGCGCTCCTG CATTGTGTTA
    GGAGGGGACT CAGCAGTGTC GGGCTTCGCA GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC
    CCCCAGCTCC TGCACTATGT GCTTGGGGCC CCACGTGAGG ATGAGGAGCA GCGAGGAGAG
    CTGACACTGA TACACAGAGC CGTTTGCCTT GATAACACTT TTGGCAATGA CTGCAGCTTG
    AGCTGTGAGG ATTGTATGAA TGGAGGCAGA TGTAACAGTG AGAACAGTGG CTGTGTTTGC
    TCACCTGGGT GGACCGGCAT CATCTGCAAC CAAACCTGCG CCCAGGGTAC ATACGGTGAT
    GGTTGTGCCA GTATCTGCAT GTGCCAGAAT GGAGGCTCCT GTGACCCCAT CACAGGGCTT
    TGTCACTGCC CACCAGGAGT CCAGGGGCCA TTTTGTGAAG ACGGTTGTCC CAGGGGATTT
    TTTGGGAAGA ACTGCAACAA ACCATGCAAC TGTGCCAACA GCGGCCACTG CCACCGGCTA
    TATGGAGCCT GCCTGTGTGA CCCTGGGCTG TATGGACGCT TCTGCCACCT CACCTGTCCC
    AGGTGGGCTT TTGGTGTGGG CTGCTCACAG GAGTGTCAGT GTGTGAAGGA GCACACACTG
    GGATGCAGCG CTCGGAACGG GACCTGCAGC TGCAAGCCTG GATATCACGG CAAGAAGTGC
    CAGAAAGCAT GCACACCTGG CTTTTATGGG GCTGGTTGCA AACTGAGGTG CAACTGTTCC
    ACCGACGTTT TGTGTGACCA CGAGACGGGA GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC
    CATGGAGAAA AGTGCCATTC CTCTTGCCAG CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG
    AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC CCAGAGACTG GGCGTTGTTT CTGCCCAGCT
    GGGAAAACTG GTGCCATGTG TGAGAGAGCC TGCCCTGTTG GATACTACGG ACAAAACTGC
    CAGCTGCCAT GCAGCTGCAG CAGTGATGCT CTGTGTCACC ACATCACTGG GGAGTGCACG
    TGTCCCCCCG GCTGGACTGG CCACGACTGC AAGCACCCCT GCAATAGCGG CCGTTGGGGA
    CAGCACTGTG AAAACTCCTG TGCCTGCAAT AACAGCGATG GGAGCTGCGA TCCTGTCACT
    GGGTCCTGCT TCTGTGAGCC AGGATTCACT GGGAAACACT GCGAGCAGAG ATGTCCTGAG
    GGAAGCTTTG GTCCCAGCTG CATGCACAGC TGCCAGTGCC AGAACGGGGC TGCATGTGAC
    CATGTGAGCG GAGCCTGCAC GTGCAGTGCT GGATGGACCG GGACCTTCTG CAGGAAAGCT
    TGTCCAGAAG GATTTTTTGG GCTTGACTGC CACCAGGTGT GCAACTGCAA GAACGCTGAG
    GGCTGTGACC ATGTCCTGGG GACGTGCCGC TGCCTCCCAG GCTGGAGGGG AGAGACCTGC
    GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG CAGGGCTGTG CTCACACCTG CAACTGTCAC
    AACGGAGCAC TCTGCGACCA CATAACGGGG ACATGTGCCT GCACCCCAGG CTGGGGAGGA
    GCCACCTGCC AAGAAGCCTG TCCCCCAGGC TGGCATGGTG CTGGCTGCCT TCAGCGCTGC
    ATTTGCCCTG GCCAGACTGC ATGTGACCCC GTGACGGGCA AGTGCCAGTG TTCCAAGGGC
    GGAACAGGGA CATCCTGTGA GCTTGCATGT CCCCCAGGAT TTTTTGGAGA GAGATGTGAA
    GAGCAATGTG ACTGCATTCA CAGCGTGTCC TGCCACCCCC AAACGGGGGC TTGTCGCTGT
    GCCAAGGGGT GGCGAGGCAG GCACTGTGAC AAACCCTGCT TGCCTGGCCA CTACGGCATG
    GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA GGCACCCCCT GCCACCACCT GACGGGTGAG
    TGCAGCTGTC CTCCAGGATT CACTGGCCAT GGCTGCGAGA AAACTTGTCC ACCAAACACG
    TATGGCCAGA GTTGCAGCCA AGCCTGTCGC TGCCGTGCTG AGGAGGACTG TCACCCCATC
    ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC CACGGGGCCC AGTGTGACCG CCCAGGGTGT
    CCAGAAGGTA CTTACGGTTC AAACTGCCAA AAATCCTGTA AATGCATGAA TGGAGGCCAT
    TGCGACCCAG TGTCGGGAAC TTGTGATTGC GCGCCTGGTT TCATTGGAGC CGACTGTAGT
    AAAACTTGCC CAGAAAATCG ATACGGGAAG GACTGCGCCC TGTTCTGTGC ATGCGGTAAA
    GGTCATTGTG ACCCACGGAC TGGCAGCTGT ACCTGTCCTC CTGGCCAAAT GGGACCTGGC
    TGTCAGCAAG TGTGTCCCCA GGGACGGTTC GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT
    CAGAACGGTG CTATCTGTGA CCACGTGGAT GGCAGCTGCA CTTGTGGACT TGGCTGGACA
    GGAAAATCGT GTGAGAAAGA ATGTCTCCCT GGCAAGTACG GCCCCAACTG CAGCTTGGAC
    TGCTCATGCC AGCACAACGG CACCTGCGAC AGGTTCACGG GCTGCTGCCT CTGCCCCGCG
    GGGTTCTACG GGCGCTCCTG TGAGCACGGG TGCCCCCCCG GGTTTTATGG GCTGAGCTGC
    CTTCATGCAT GTGCCTGTAA AAACGGAGCG AGCTGCGATG CCGTGATGGG ACAGTGCATT
    TGTCCCTCGG GGTACCATGG TATCCACTGT GAGAAAGGCT GTGCCAGTGG GCGCTTCGGT
    GAGCGGTGCC AGCGCCAGTG CGACTGTGGA GGAGCTCCCT GCGACCCGGC CACTGGGAAA
    TGCCTCTGCC CACCTGGAAA GACTGGGGAC AAGTGTGACA TTGGCTGCCG GTCTGACCAG
    TACGGCCCCG ACTGCTCTCT GCGATGCCAG TGTGCCAGCA AGTCCCAGTG CAACCCATAC
    AATGGGAAAT GTGTCTGTCC AGCCACGTGG ATGGGGCCAA CCTGCAAGGA AGGTGGCCCT
    CCAAAGCTTC CTTTTTATGA AGAACAAACT CAAGAAAGAG GGAATATTTT TCCGAGAGCT
    CTACAACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40125
    Clone ID Related Accession (Same CDS sequence) XM_015291747.1
    Accession Version XM_015291747.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4230bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGTGAAGA TGTGGAAACA AGGCTGCACA GGGAGGAGAT GGTGCATGGG ATATGAGAGG 
    AGGTCTGGTT ACTACACTGT GTATAAGCAG GTGTACAGAA TGGAGCACCA GACTGTCTAT
    AAGTGCTGCC CTGGGTGGGC TCAACAGAAC AGCGAGCTTG GCTGCTTGCA CATGCTCTGT
    GCTGCTGACA CTTGCTTCAA TGGAGGGAAG TGTTCTGAAG AAGGACCTCA CATGTGCCAG
    TGTCCTGCGG GATTTCAGGG TCCTCGCTGC CAGTATGATG TAAATGAGTG CAGCACTGAG
    AACGGAGGCT GTCAGGACCA GTGCTGTAAC ACCATCGGCA GCTACCACTG CAAATGTCCA
    GCTGGCCAGA AACTGGGGGA AGATGGAAAA TCGTGCGGAG ATATAGATGA GTGTGCAGAG
    GTGAATGGAG GCTGCCAGCA GGGCTGCATC AACACCCACA GCTCTTTCCA CTGCCACTGT
    TGGGCAGGAT ACAGGCTGCA CTCTGATGGG CGCACGTGCT TGATGATAGA CCCCTGCACA
    AGTGGGAATG GAGGATGCTC ACAAATCTGT CAAAATGAGA GAGGAACTGC CAAATGTGAC
    TGTCACCCAG GGCATTATCT TTCTACAGAT GAGAAGTCCT GCTTAGACAT TGATGAGTGT
    GCAGAAGGGA AAGCCAGGTG TGCTCATCAC TGTGTGAACA TGCTGGGATC CTTCACCTGT
    GCCTGTAATC CCGGCTTCGA GCTGGGGGCT GATGGGAAGC AGTGCTACCG CATTGAAATG
    GAGATAGTGG ACAGCTGCCA GCAGGACAAC GGTGGGTGCT CCCACCGCTG CGAGCACACA
    GCAGCAGGAC CACGCTGCTC CTGCCACGAT GGCTACGTGC TCAGCTCAGA TGGCAAGGCA
    TGCACAGACC TGGATGAGTG CGAGACCGGC GAGTCGTGCT GCTCCCAGTT CTGCATCAAC
    TACGTGGGCA GCTACGAGTG CACCTGTAGA GCAGGCTTCC AGCCCAGTGC CGATGGCTGC
    AGCTGCGACG ATGTGGATGA ATGTCGTCTT GAGAATGGCA ACTGTGACCA TATCTGCATT
    AATTCCCTGG GGTCATACGA ATGCGCCTGC AAGGAGAGGT ACCGGCTGGG TGCTGACCGG
    CGCTCCTGCA TTGTGTTAGG AGGGGACTCA GCAGTGTCGG GCTTCGCAGG GGCTCCGGGG
    CTGCAGTTCA GAGGTGCCCC CCAGCTCCTG CACTATGTGC TTGGGGCCCC ACGTGAGGAT
    GAGGAGCAGC GAGGAGAGCT GACACTGATA CACAGAGCCG TTTGCCTTGA TAACACTTTT
    GGCAATGACT GCAGCTTGAG CTGTGAGGAT TGTATGAATG GAGGCAGATG TAACAGTGAG
    AACAGTGGCT GTGTTTGCTC ACCTGGGTGG ACCGGCATCA TCTGCAACCA AACCTGCGCC
    CAGGGTACAT ACGGTGATGG TTGTGCCAGT ATCTGCATGT GCCAGAATGG AGGCTCCTGT
    GACCCCATCA CAGGGCTTTG TCACTGCCCA CCAGGAGTCC AGGGGCCATT TTGTGAAGAC
    GGTTGTCCCA GGGGATTTTT TGGGAAGAAC TGCAACAAAC CATGCAACTG TGCCAACAGC
    GGCCACTGCC ACCGGCTATA TGGAGCCTGC CTGTGTGACC CTGGGCTGTA TGGACGCTTC
    TGCCACCTCA CCTGTCCCAG GTGGGCTTTT GGTGTGGGCT GCTCACAGGA GTGTCAGTGT
    GTGAAGGAGC ACACACTGGG ATGCAGCGCT CGGAACGGGA CCTGCAGCTG CAAGCCTGGA
    TATCACGGCA AGAAGTGCCA GAAAGCATGC ACACCTGGCT TTTATGGGGC TGGTTGCAAA
    CTGAGGTGCA ACTGTTCCAC CGACGTTTTG TGTGACCACG AGACGGGAGC CTGCAAGCGT
    CCGTGCCCGC CTGGTTTCCA TGGAGAAAAG TGCCATTCCT CTTGCCAGCC CGGGAGCTTT
    GGGGTGGACT GCAGGCAGAG CTGCAGCTGC GGGGGAGCAC CGTGTGACCC AGAGACTGGG
    CGTTGTTTCT GCCCAGCTGG GAAAACTGGT GCCATGTGTG AGAGAGCCTG CCCTGTTGGA
    TACTACGGAC AAAACTGCCA GCTGCCATGC AGCTGCAGCA GTGATGCTCT GTGTCACCAC
    ATCACTGGGG AGTGCACGTG TCCCCCCGGC TGGACTGGCC ACGACTGCAA GCACCCCTGC
    AATAGCGGCC GTTGGGGACA GCACTGTGAA AACTCCTGTG CCTGCAATAA CAGCGATGGG
    AGCTGCGATC CTGTCACTGG GTCCTGCTTC TGTGAGCCAG GATTCACTGG GAAACACTGC
    GAGCAGAGAT GTCCTGAGGG AAGCTTTGGT CCCAGCTGCA TGCACAGCTG CCAGTGCCAG
    AACGGGGCTG CATGTGACCA TGTGAGCGGA GCCTGCACGT GCAGTGCTGG ATGGACCGGG
    ACCTTCTGCA GGAAAGCTTG TCCAGAAGGA TTTTTTGGGC TTGACTGCCA CCAGGTGTGC
    AACTGCAAGA ACGCTGAGGG CTGTGACCAT GTCCTGGGGA CGTGCCGCTG CCTCCCAGGC
    TGGAGGGGAG AGACCTGCGA GCAGCCGTGT CAGGGGGACA GGTTTGGGCA GGGCTGTGCT
    CACACCTGCA ACTGTCACAA CGGAGCACTC TGCGACCACA TAACGGGGAC ATGTGCCTGC
    ACCCCAGGCT GGGGAGGAGC CACCTGCCAA GAAGCCTGTC CCCCAGGCTG GCATGGTGCT
    GGCTGCCTTC AGCGCTGCAT TTGCCCTGGC CAGACTGCAT GTGACCCCGT GACGGGCAAG
    TGCCAGTGTT CCAAGGGCGG AACAGGGACA TCCTGTGAGC TTGCATGTCC CCCAGGATTT
    TTTGGAGAGA GATGTGAAGA GCAATGTGAC TGCATTCACA GCGTGTCCTG CCACCCCCAA
    ACGGGGGCTT GTCGCTGTGC CAAGGGGTGG CGAGGCAGGC ACTGTGACAA ACCCTGCTTG
    CCTGGCCACT ACGGCATGGG TTGTGCCCGG CGGTGCCACT GCCCTGCAGG CACCCCCTGC
    CACCACCTGA CGGGTGAGTG CAGCTGTCCT CCAGGATTCA CTGGCCATGG CTGCGAGAAA
    ACTTGTCCAC CAAACACGTA TGGCCAGAGT TGCAGCCAAG CCTGTCGCTG CCGTGCTGAG
    GAGGACTGTC ACCCCATCAC TGGGCGGTGC GTGTGCCGCC TGGGGTACCA CGGGGCCCAG
    TGTGACCGCC CAGGGTGTCC AGAAGGTACT TACGGTTCAA ACTGCCAAAA ATCCTGTAAA
    TGCATGAATG GAGGCCATTG CGACCCAGTG TCGGGAACTT GTGATTGCGC GCCTGGTTTC
    ATTGGAGCCG ACTGTAGTAA AACTTGCCCA GAAAATCGAT ACGGGAAGGA CTGCGCCCTG
    TTCTGTGCAT GCGGTAAAGG TCATTGTGAC CCACGGACTG GCAGCTGTAC CTGTCCTCCT
    GGCCAAATGG GACCTGGCTG TCAGCAAGTG TGTCCCCAGG GACGGTTCGG CCCCAGCTGC
    CGCCTGACAT GCAGCTGTCA GAACGGTGCT ATCTGTGACC ACGTGGATGG CAGCTGCACT
    TGTGGACTTG GCTGGACAGG AAAATCGTGT GAGAAAGAAT GTCTCCCTGG CAAGTACGGC
    CCCAACTGCA GCTTGGACTG CTCATGCCAG CACAACGGCA CCTGCGACAG GTTCACGGGC
    TGCTGCCTCT GCCCCGCGGG GTTCTACGGG CGCTCCTGTG AGCACGGGTG CCCCCCCGGG
    TTTTATGGGC TGAGCTGCCT TCATGCATGT GCCTGTAAAA ACGGAGCGAG CTGCGATGCC
    GTGATGGGAC AGTGCATTTG TCCCTCGGGG TACCATGGTA TCCACTGTGA GAAAGGCTGT
    GCCAGTGGGC GCTTCGGTGA GCGGTGCCAG CGCCAGTGCG ACTGTGGAGG AGCTCCCTGC
    GACCCGGCCA CTGGGAAATG CCTCTGCCCA CCTGGAAAGA CTGGGGACAA GTGTGACATT
    GGCTGCCGGT CTGACCAGTA CGGCCCCGAC TGCTCTCTGC GATGCCAGTG TGCCAGCAAG
    TCCCAGTGCA ACCCATACAA TGGGAAATGT GTCTGTCCAG CCACGTGGAT GGGGCCAACC
    TGCAAGGAAG GTGGCCCTCC AAAGCTTCCT TTTTATGAAG AACAAACTCA AGAAAGAGGG
    AATATTTTTC CGAGAGCTCT ACAACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147233.1
    CDS552..4781
    Translation

    Target ORF information:

    RefSeq Version XM_015291747.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291747.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGTGAAGA TGTGGAAACA AGGCTGCACA GGGAGGAGAT GGTGCATGGG ATATGAGAGG 
    AGGTCTGGTT ACTACACTGT GTATAAGCAG GTGTACAGAA TGGAGCACCA GACTGTCTAT
    AAGTGCTGCC CTGGGTGGGC TCAACAGAAC AGCGAGCTTG GCTGCTTGCA CATGCTCTGT
    GCTGCTGACA CTTGCTTCAA TGGAGGGAAG TGTTCTGAAG AAGGACCTCA CATGTGCCAG
    TGTCCTGCGG GATTTCAGGG TCCTCGCTGC CAGTATGATG TAAATGAGTG CAGCACTGAG
    AACGGAGGCT GTCAGGACCA GTGCTGTAAC ACCATCGGCA GCTACCACTG CAAATGTCCA
    GCTGGCCAGA AACTGGGGGA AGATGGAAAA TCGTGCGGAG ATATAGATGA GTGTGCAGAG
    GTGAATGGAG GCTGCCAGCA GGGCTGCATC AACACCCACA GCTCTTTCCA CTGCCACTGT
    TGGGCAGGAT ACAGGCTGCA CTCTGATGGG CGCACGTGCT TGATGATAGA CCCCTGCACA
    AGTGGGAATG GAGGATGCTC ACAAATCTGT CAAAATGAGA GAGGAACTGC CAAATGTGAC
    TGTCACCCAG GGCATTATCT TTCTACAGAT GAGAAGTCCT GCTTAGACAT TGATGAGTGT
    GCAGAAGGGA AAGCCAGGTG TGCTCATCAC TGTGTGAACA TGCTGGGATC CTTCACCTGT
    GCCTGTAATC CCGGCTTCGA GCTGGGGGCT GATGGGAAGC AGTGCTACCG CATTGAAATG
    GAGATAGTGG ACAGCTGCCA GCAGGACAAC GGTGGGTGCT CCCACCGCTG CGAGCACACA
    GCAGCAGGAC CACGCTGCTC CTGCCACGAT GGCTACGTGC TCAGCTCAGA TGGCAAGGCA
    TGCACAGACC TGGATGAGTG CGAGACCGGC GAGTCGTGCT GCTCCCAGTT CTGCATCAAC
    TACGTGGGCA GCTACGAGTG CACCTGTAGA GCAGGCTTCC AGCCCAGTGC CGATGGCTGC
    AGCTGCGACG ATGTGGATGA ATGTCGTCTT GAGAATGGCA ACTGTGACCA TATCTGCATT
    AATTCCCTGG GGTCATACGA ATGCGCCTGC AAGGAGAGGT ACCGGCTGGG TGCTGACCGG
    CGCTCCTGCA TTGTGTTAGG AGGGGACTCA GCAGTGTCGG GCTTCGCAGG GGCTCCGGGG
    CTGCAGTTCA GAGGTGCCCC CCAGCTCCTG CACTATGTGC TTGGGGCCCC ACGTGAGGAT
    GAGGAGCAGC GAGGAGAGCT GACACTGATA CACAGAGCCG TTTGCCTTGA TAACACTTTT
    GGCAATGACT GCAGCTTGAG CTGTGAGGAT TGTATGAATG GAGGCAGATG TAACAGTGAG
    AACAGTGGCT GTGTTTGCTC ACCTGGGTGG ACCGGCATCA TCTGCAACCA AACCTGCGCC
    CAGGGTACAT ACGGTGATGG TTGTGCCAGT ATCTGCATGT GCCAGAATGG AGGCTCCTGT
    GACCCCATCA CAGGGCTTTG TCACTGCCCA CCAGGAGTCC AGGGGCCATT TTGTGAAGAC
    GGTTGTCCCA GGGGATTTTT TGGGAAGAAC TGCAACAAAC CATGCAACTG TGCCAACAGC
    GGCCACTGCC ACCGGCTATA TGGAGCCTGC CTGTGTGACC CTGGGCTGTA TGGACGCTTC
    TGCCACCTCA CCTGTCCCAG GTGGGCTTTT GGTGTGGGCT GCTCACAGGA GTGTCAGTGT
    GTGAAGGAGC ACACACTGGG ATGCAGCGCT CGGAACGGGA CCTGCAGCTG CAAGCCTGGA
    TATCACGGCA AGAAGTGCCA GAAAGCATGC ACACCTGGCT TTTATGGGGC TGGTTGCAAA
    CTGAGGTGCA ACTGTTCCAC CGACGTTTTG TGTGACCACG AGACGGGAGC CTGCAAGCGT
    CCGTGCCCGC CTGGTTTCCA TGGAGAAAAG TGCCATTCCT CTTGCCAGCC CGGGAGCTTT
    GGGGTGGACT GCAGGCAGAG CTGCAGCTGC GGGGGAGCAC CGTGTGACCC AGAGACTGGG
    CGTTGTTTCT GCCCAGCTGG GAAAACTGGT GCCATGTGTG AGAGAGCCTG CCCTGTTGGA
    TACTACGGAC AAAACTGCCA GCTGCCATGC AGCTGCAGCA GTGATGCTCT GTGTCACCAC
    ATCACTGGGG AGTGCACGTG TCCCCCCGGC TGGACTGGCC ACGACTGCAA GCACCCCTGC
    AATAGCGGCC GTTGGGGACA GCACTGTGAA AACTCCTGTG CCTGCAATAA CAGCGATGGG
    AGCTGCGATC CTGTCACTGG GTCCTGCTTC TGTGAGCCAG GATTCACTGG GAAACACTGC
    GAGCAGAGAT GTCCTGAGGG AAGCTTTGGT CCCAGCTGCA TGCACAGCTG CCAGTGCCAG
    AACGGGGCTG CATGTGACCA TGTGAGCGGA GCCTGCACGT GCAGTGCTGG ATGGACCGGG
    ACCTTCTGCA GGAAAGCTTG TCCAGAAGGA TTTTTTGGGC TTGACTGCCA CCAGGTGTGC
    AACTGCAAGA ACGCTGAGGG CTGTGACCAT GTCCTGGGGA CGTGCCGCTG CCTCCCAGGC
    TGGAGGGGAG AGACCTGCGA GCAGCCGTGT CAGGGGGACA GGTTTGGGCA GGGCTGTGCT
    CACACCTGCA ACTGTCACAA CGGAGCACTC TGCGACCACA TAACGGGGAC ATGTGCCTGC
    ACCCCAGGCT GGGGAGGAGC CACCTGCCAA GAAGCCTGTC CCCCAGGCTG GCATGGTGCT
    GGCTGCCTTC AGCGCTGCAT TTGCCCTGGC CAGACTGCAT GTGACCCCGT GACGGGCAAG
    TGCCAGTGTT CCAAGGGCGG AACAGGGACA TCCTGTGAGC TTGCATGTCC CCCAGGATTT
    TTTGGAGAGA GATGTGAAGA GCAATGTGAC TGCATTCACA GCGTGTCCTG CCACCCCCAA
    ACGGGGGCTT GTCGCTGTGC CAAGGGGTGG CGAGGCAGGC ACTGTGACAA ACCCTGCTTG
    CCTGGCCACT ACGGCATGGG TTGTGCCCGG CGGTGCCACT GCCCTGCAGG CACCCCCTGC
    CACCACCTGA CGGGTGAGTG CAGCTGTCCT CCAGGATTCA CTGGCCATGG CTGCGAGAAA
    ACTTGTCCAC CAAACACGTA TGGCCAGAGT TGCAGCCAAG CCTGTCGCTG CCGTGCTGAG
    GAGGACTGTC ACCCCATCAC TGGGCGGTGC GTGTGCCGCC TGGGGTACCA CGGGGCCCAG
    TGTGACCGCC CAGGGTGTCC AGAAGGTACT TACGGTTCAA ACTGCCAAAA ATCCTGTAAA
    TGCATGAATG GAGGCCATTG CGACCCAGTG TCGGGAACTT GTGATTGCGC GCCTGGTTTC
    ATTGGAGCCG ACTGTAGTAA AACTTGCCCA GAAAATCGAT ACGGGAAGGA CTGCGCCCTG
    TTCTGTGCAT GCGGTAAAGG TCATTGTGAC CCACGGACTG GCAGCTGTAC CTGTCCTCCT
    GGCCAAATGG GACCTGGCTG TCAGCAAGTG TGTCCCCAGG GACGGTTCGG CCCCAGCTGC
    CGCCTGACAT GCAGCTGTCA GAACGGTGCT ATCTGTGACC ACGTGGATGG CAGCTGCACT
    TGTGGACTTG GCTGGACAGG AAAATCGTGT GAGAAAGAAT GTCTCCCTGG CAAGTACGGC
    CCCAACTGCA GCTTGGACTG CTCATGCCAG CACAACGGCA CCTGCGACAG GTTCACGGGC
    TGCTGCCTCT GCCCCGCGGG GTTCTACGGG CGCTCCTGTG AGCACGGGTG CCCCCCCGGG
    TTTTATGGGC TGAGCTGCCT TCATGCATGT GCCTGTAAAA ACGGAGCGAG CTGCGATGCC
    GTGATGGGAC AGTGCATTTG TCCCTCGGGG TACCATGGTA TCCACTGTGA GAAAGGCTGT
    GCCAGTGGGC GCTTCGGTGA GCGGTGCCAG CGCCAGTGCG ACTGTGGAGG AGCTCCCTGC
    GACCCGGCCA CTGGGAAATG CCTCTGCCCA CCTGGAAAGA CTGGGGACAA GTGTGACATT
    GGCTGCCGGT CTGACCAGTA CGGCCCCGAC TGCTCTCTGC GATGCCAGTG TGCCAGCAAG
    TCCCAGTGCA ACCCATACAA TGGGAAATGT GTCTGTCCAG CCACGTGGAT GGGGCCAACC
    TGCAAGGAAG GTGGCCCTCC AAAGCTTCCT TTTTATGAAG AACAAACTCA AGAAAGAGGG
    AATATTTTTC CGAGAGCTCT ACAACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40126
    Clone ID Related Accession (Same CDS sequence) XM_015291748.1 , XM_015291748.2 , XM_025153583.1
    Accession Version XM_015291748.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3879bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG GGACGGTTCG GCCCCAGCTG
    CCGCCTGACA TGCAGCTGTC AGAACGGTGC TATCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147234.1
    CDS179..4057
    Translation

    Target ORF information:

    RefSeq Version XM_015291748.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291748.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG GGACGGTTCG GCCCCAGCTG
    CCGCCTGACA TGCAGCTGTC AGAACGGTGC TATCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40126
    Clone ID Related Accession (Same CDS sequence) XM_015291748.1 , XM_015291748.2 , XM_025153583.1
    Accession Version XM_015291748.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3879bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291748.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG GGACGGTTCG GCCCCAGCTG
    CCGCCTGACA TGCAGCTGTC AGAACGGTGC TATCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147234.1
    CDS183..4061
    Translation

    Target ORF information:

    RefSeq Version XM_015291748.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291748.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG GGACGGTTCG GCCCCAGCTG
    CCGCCTGACA TGCAGCTGTC AGAACGGTGC TATCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40126
    Clone ID Related Accession (Same CDS sequence) XM_015291748.1 , XM_015291748.2 , XM_025153583.1
    Accession Version XM_025153583.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3879bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG GGACGGTTCG GCCCCAGCTG
    CCGCCTGACA TGCAGCTGTC AGAACGGTGC TATCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009351.1
    CDS184..4062
    Translation

    Target ORF information:

    RefSeq Version XM_025153583.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153583.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG GGACGGTTCG GCCCCAGCTG
    CCGCCTGACA TGCAGCTGTC AGAACGGTGC TATCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152774
    Clone ID Related Accession (Same CDS sequence) XM_025153565.1 , XM_025153571.1 , XM_025153564.1 , XM_025153568.1 , XM_025153569.1 , XM_025153574.1 , XM_025153567.1 , XM_025153573.1 , XM_025153570.1 , XM_025153572.1
    Accession Version XM_025153565.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGC AGAATGTCTC
    CCTGGCAAGT ACGGCCCCAA CTGCAGCTTG GACTGCTCAT GCCAGCACAA CGGCACCTGC
    GACAGGTTCA CGGGCTGCTG CCTCTGCCCC GCGGGGTTCT ACGGGCGCTC CTGTGAGCAC
    GGGTGCCCCC CCGGGTTTTA TGGGCTGAGC TGCCTTCATG CATGTGCCTG TAAAAACGGA
    GCGAGCTGCG ATGCCGTGAT GGGACAGTGC ATTTGTCCCT CGGGGTACCA TGGTATCCAC
    TGTGAGAAAG GCTGTGCCAG TGGGCGCTTC GGTGAGCGGT GCCAGCGCCA GTGCGACTGT
    GGAGGAGCTC CCTGCGACCC GGCCACTGGG AAATGCCTCT GCCCACCTGG AAAGACTGGG
    GACAAGTGTG ACATTGGCTG CCGGTCTGAC CAGTACGGCC CCGACTGCTC TCTGCGATGC
    CAGTGTGCCA GCAAGTCCCA GTGCAACCCA TACAATGGGA AATGTGTCTG TCCAGCCACG
    TGGATGGGGC CAACCTGCAA GGAAGGTGGC CCTCCAAAGC TTCCTTTTTA TGAAGAACAA
    ACTCAAGAAA GAGGGAATAT TTTTCCGAGA GCTCTACAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009333.1
    CDS179..4723
    Translation

    Target ORF information:

    RefSeq Version XM_025153565.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153565.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGC AGAATGTCTC
    CCTGGCAAGT ACGGCCCCAA CTGCAGCTTG GACTGCTCAT GCCAGCACAA CGGCACCTGC
    GACAGGTTCA CGGGCTGCTG CCTCTGCCCC GCGGGGTTCT ACGGGCGCTC CTGTGAGCAC
    GGGTGCCCCC CCGGGTTTTA TGGGCTGAGC TGCCTTCATG CATGTGCCTG TAAAAACGGA
    GCGAGCTGCG ATGCCGTGAT GGGACAGTGC ATTTGTCCCT CGGGGTACCA TGGTATCCAC
    TGTGAGAAAG GCTGTGCCAG TGGGCGCTTC GGTGAGCGGT GCCAGCGCCA GTGCGACTGT
    GGAGGAGCTC CCTGCGACCC GGCCACTGGG AAATGCCTCT GCCCACCTGG AAAGACTGGG
    GACAAGTGTG ACATTGGCTG CCGGTCTGAC CAGTACGGCC CCGACTGCTC TCTGCGATGC
    CAGTGTGCCA GCAAGTCCCA GTGCAACCCA TACAATGGGA AATGTGTCTG TCCAGCCACG
    TGGATGGGGC CAACCTGCAA GGAAGGTGGC CCTCCAAAGC TTCCTTTTTA TGAAGAACAA
    ACTCAAGAAA GAGGGAATAT TTTTCCGAGA GCTCTACAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152774
    Clone ID Related Accession (Same CDS sequence) XM_025153565.1 , XM_025153571.1 , XM_025153564.1 , XM_025153568.1 , XM_025153569.1 , XM_025153574.1 , XM_025153567.1 , XM_025153573.1 , XM_025153570.1 , XM_025153572.1
    Accession Version XM_025153571.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGC AGAATGTCTC
    CCTGGCAAGT ACGGCCCCAA CTGCAGCTTG GACTGCTCAT GCCAGCACAA CGGCACCTGC
    GACAGGTTCA CGGGCTGCTG CCTCTGCCCC GCGGGGTTCT ACGGGCGCTC CTGTGAGCAC
    GGGTGCCCCC CCGGGTTTTA TGGGCTGAGC TGCCTTCATG CATGTGCCTG TAAAAACGGA
    GCGAGCTGCG ATGCCGTGAT GGGACAGTGC ATTTGTCCCT CGGGGTACCA TGGTATCCAC
    TGTGAGAAAG GCTGTGCCAG TGGGCGCTTC GGTGAGCGGT GCCAGCGCCA GTGCGACTGT
    GGAGGAGCTC CCTGCGACCC GGCCACTGGG AAATGCCTCT GCCCACCTGG AAAGACTGGG
    GACAAGTGTG ACATTGGCTG CCGGTCTGAC CAGTACGGCC CCGACTGCTC TCTGCGATGC
    CAGTGTGCCA GCAAGTCCCA GTGCAACCCA TACAATGGGA AATGTGTCTG TCCAGCCACG
    TGGATGGGGC CAACCTGCAA GGAAGGTGGC CCTCCAAAGC TTCCTTTTTA TGAAGAACAA
    ACTCAAGAAA GAGGGAATAT TTTTCCGAGA GCTCTACAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009339.1
    CDS179..4723
    Translation

    Target ORF information:

    RefSeq Version XM_025153571.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153571.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGC AGAATGTCTC
    CCTGGCAAGT ACGGCCCCAA CTGCAGCTTG GACTGCTCAT GCCAGCACAA CGGCACCTGC
    GACAGGTTCA CGGGCTGCTG CCTCTGCCCC GCGGGGTTCT ACGGGCGCTC CTGTGAGCAC
    GGGTGCCCCC CCGGGTTTTA TGGGCTGAGC TGCCTTCATG CATGTGCCTG TAAAAACGGA
    GCGAGCTGCG ATGCCGTGAT GGGACAGTGC ATTTGTCCCT CGGGGTACCA TGGTATCCAC
    TGTGAGAAAG GCTGTGCCAG TGGGCGCTTC GGTGAGCGGT GCCAGCGCCA GTGCGACTGT
    GGAGGAGCTC CCTGCGACCC GGCCACTGGG AAATGCCTCT GCCCACCTGG AAAGACTGGG
    GACAAGTGTG ACATTGGCTG CCGGTCTGAC CAGTACGGCC CCGACTGCTC TCTGCGATGC
    CAGTGTGCCA GCAAGTCCCA GTGCAACCCA TACAATGGGA AATGTGTCTG TCCAGCCACG
    TGGATGGGGC CAACCTGCAA GGAAGGTGGC CCTCCAAAGC TTCCTTTTTA TGAAGAACAA
    ACTCAAGAAA GAGGGAATAT TTTTCCGAGA GCTCTACAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152774
    Clone ID Related Accession (Same CDS sequence) XM_025153565.1 , XM_025153571.1 , XM_025153564.1 , XM_025153568.1 , XM_025153569.1 , XM_025153574.1 , XM_025153567.1 , XM_025153573.1 , XM_025153570.1 , XM_025153572.1
    Accession Version XM_025153564.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGC AGAATGTCTC
    CCTGGCAAGT ACGGCCCCAA CTGCAGCTTG GACTGCTCAT GCCAGCACAA CGGCACCTGC
    GACAGGTTCA CGGGCTGCTG CCTCTGCCCC GCGGGGTTCT ACGGGCGCTC CTGTGAGCAC
    GGGTGCCCCC CCGGGTTTTA TGGGCTGAGC TGCCTTCATG CATGTGCCTG TAAAAACGGA
    GCGAGCTGCG ATGCCGTGAT GGGACAGTGC ATTTGTCCCT CGGGGTACCA TGGTATCCAC
    TGTGAGAAAG GCTGTGCCAG TGGGCGCTTC GGTGAGCGGT GCCAGCGCCA GTGCGACTGT
    GGAGGAGCTC CCTGCGACCC GGCCACTGGG AAATGCCTCT GCCCACCTGG AAAGACTGGG
    GACAAGTGTG ACATTGGCTG CCGGTCTGAC CAGTACGGCC CCGACTGCTC TCTGCGATGC
    CAGTGTGCCA GCAAGTCCCA GTGCAACCCA TACAATGGGA AATGTGTCTG TCCAGCCACG
    TGGATGGGGC CAACCTGCAA GGAAGGTGGC CCTCCAAAGC TTCCTTTTTA TGAAGAACAA
    ACTCAAGAAA GAGGGAATAT TTTTCCGAGA GCTCTACAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009332.1
    CDS179..4723
    Translation

    Target ORF information:

    RefSeq Version XM_025153564.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153564.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGC AGAATGTCTC
    CCTGGCAAGT ACGGCCCCAA CTGCAGCTTG GACTGCTCAT GCCAGCACAA CGGCACCTGC
    GACAGGTTCA CGGGCTGCTG CCTCTGCCCC GCGGGGTTCT ACGGGCGCTC CTGTGAGCAC
    GGGTGCCCCC CCGGGTTTTA TGGGCTGAGC TGCCTTCATG CATGTGCCTG TAAAAACGGA
    GCGAGCTGCG ATGCCGTGAT GGGACAGTGC ATTTGTCCCT CGGGGTACCA TGGTATCCAC
    TGTGAGAAAG GCTGTGCCAG TGGGCGCTTC GGTGAGCGGT GCCAGCGCCA GTGCGACTGT
    GGAGGAGCTC CCTGCGACCC GGCCACTGGG AAATGCCTCT GCCCACCTGG AAAGACTGGG
    GACAAGTGTG ACATTGGCTG CCGGTCTGAC CAGTACGGCC CCGACTGCTC TCTGCGATGC
    CAGTGTGCCA GCAAGTCCCA GTGCAACCCA TACAATGGGA AATGTGTCTG TCCAGCCACG
    TGGATGGGGC CAACCTGCAA GGAAGGTGGC CCTCCAAAGC TTCCTTTTTA TGAAGAACAA
    ACTCAAGAAA GAGGGAATAT TTTTCCGAGA GCTCTACAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152774
    Clone ID Related Accession (Same CDS sequence) XM_025153565.1 , XM_025153571.1 , XM_025153564.1 , XM_025153568.1 , XM_025153569.1 , XM_025153574.1 , XM_025153567.1 , XM_025153573.1 , XM_025153570.1 , XM_025153572.1
    Accession Version XM_025153568.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGC AGAATGTCTC
    CCTGGCAAGT ACGGCCCCAA CTGCAGCTTG GACTGCTCAT GCCAGCACAA CGGCACCTGC
    GACAGGTTCA CGGGCTGCTG CCTCTGCCCC GCGGGGTTCT ACGGGCGCTC CTGTGAGCAC
    GGGTGCCCCC CCGGGTTTTA TGGGCTGAGC TGCCTTCATG CATGTGCCTG TAAAAACGGA
    GCGAGCTGCG ATGCCGTGAT GGGACAGTGC ATTTGTCCCT CGGGGTACCA TGGTATCCAC
    TGTGAGAAAG GCTGTGCCAG TGGGCGCTTC GGTGAGCGGT GCCAGCGCCA GTGCGACTGT
    GGAGGAGCTC CCTGCGACCC GGCCACTGGG AAATGCCTCT GCCCACCTGG AAAGACTGGG
    GACAAGTGTG ACATTGGCTG CCGGTCTGAC CAGTACGGCC CCGACTGCTC TCTGCGATGC
    CAGTGTGCCA GCAAGTCCCA GTGCAACCCA TACAATGGGA AATGTGTCTG TCCAGCCACG
    TGGATGGGGC CAACCTGCAA GGAAGGTGGC CCTCCAAAGC TTCCTTTTTA TGAAGAACAA
    ACTCAAGAAA GAGGGAATAT TTTTCCGAGA GCTCTACAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009336.1
    CDS179..4723
    Translation

    Target ORF information:

    RefSeq Version XM_025153568.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153568.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGC AGAATGTCTC
    CCTGGCAAGT ACGGCCCCAA CTGCAGCTTG GACTGCTCAT GCCAGCACAA CGGCACCTGC
    GACAGGTTCA CGGGCTGCTG CCTCTGCCCC GCGGGGTTCT ACGGGCGCTC CTGTGAGCAC
    GGGTGCCCCC CCGGGTTTTA TGGGCTGAGC TGCCTTCATG CATGTGCCTG TAAAAACGGA
    GCGAGCTGCG ATGCCGTGAT GGGACAGTGC ATTTGTCCCT CGGGGTACCA TGGTATCCAC
    TGTGAGAAAG GCTGTGCCAG TGGGCGCTTC GGTGAGCGGT GCCAGCGCCA GTGCGACTGT
    GGAGGAGCTC CCTGCGACCC GGCCACTGGG AAATGCCTCT GCCCACCTGG AAAGACTGGG
    GACAAGTGTG ACATTGGCTG CCGGTCTGAC CAGTACGGCC CCGACTGCTC TCTGCGATGC
    CAGTGTGCCA GCAAGTCCCA GTGCAACCCA TACAATGGGA AATGTGTCTG TCCAGCCACG
    TGGATGGGGC CAACCTGCAA GGAAGGTGGC CCTCCAAAGC TTCCTTTTTA TGAAGAACAA
    ACTCAAGAAA GAGGGAATAT TTTTCCGAGA GCTCTACAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152774
    Clone ID Related Accession (Same CDS sequence) XM_025153565.1 , XM_025153571.1 , XM_025153564.1 , XM_025153568.1 , XM_025153569.1 , XM_025153574.1 , XM_025153567.1 , XM_025153573.1 , XM_025153570.1 , XM_025153572.1
    Accession Version XM_025153569.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGC AGAATGTCTC
    CCTGGCAAGT ACGGCCCCAA CTGCAGCTTG GACTGCTCAT GCCAGCACAA CGGCACCTGC
    GACAGGTTCA CGGGCTGCTG CCTCTGCCCC GCGGGGTTCT ACGGGCGCTC CTGTGAGCAC
    GGGTGCCCCC CCGGGTTTTA TGGGCTGAGC TGCCTTCATG CATGTGCCTG TAAAAACGGA
    GCGAGCTGCG ATGCCGTGAT GGGACAGTGC ATTTGTCCCT CGGGGTACCA TGGTATCCAC
    TGTGAGAAAG GCTGTGCCAG TGGGCGCTTC GGTGAGCGGT GCCAGCGCCA GTGCGACTGT
    GGAGGAGCTC CCTGCGACCC GGCCACTGGG AAATGCCTCT GCCCACCTGG AAAGACTGGG
    GACAAGTGTG ACATTGGCTG CCGGTCTGAC CAGTACGGCC CCGACTGCTC TCTGCGATGC
    CAGTGTGCCA GCAAGTCCCA GTGCAACCCA TACAATGGGA AATGTGTCTG TCCAGCCACG
    TGGATGGGGC CAACCTGCAA GGAAGGTGGC CCTCCAAAGC TTCCTTTTTA TGAAGAACAA
    ACTCAAGAAA GAGGGAATAT TTTTCCGAGA GCTCTACAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009337.1
    CDS179..4723
    Translation

    Target ORF information:

    RefSeq Version XM_025153569.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153569.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGC AGAATGTCTC
    CCTGGCAAGT ACGGCCCCAA CTGCAGCTTG GACTGCTCAT GCCAGCACAA CGGCACCTGC
    GACAGGTTCA CGGGCTGCTG CCTCTGCCCC GCGGGGTTCT ACGGGCGCTC CTGTGAGCAC
    GGGTGCCCCC CCGGGTTTTA TGGGCTGAGC TGCCTTCATG CATGTGCCTG TAAAAACGGA
    GCGAGCTGCG ATGCCGTGAT GGGACAGTGC ATTTGTCCCT CGGGGTACCA TGGTATCCAC
    TGTGAGAAAG GCTGTGCCAG TGGGCGCTTC GGTGAGCGGT GCCAGCGCCA GTGCGACTGT
    GGAGGAGCTC CCTGCGACCC GGCCACTGGG AAATGCCTCT GCCCACCTGG AAAGACTGGG
    GACAAGTGTG ACATTGGCTG CCGGTCTGAC CAGTACGGCC CCGACTGCTC TCTGCGATGC
    CAGTGTGCCA GCAAGTCCCA GTGCAACCCA TACAATGGGA AATGTGTCTG TCCAGCCACG
    TGGATGGGGC CAACCTGCAA GGAAGGTGGC CCTCCAAAGC TTCCTTTTTA TGAAGAACAA
    ACTCAAGAAA GAGGGAATAT TTTTCCGAGA GCTCTACAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152774
    Clone ID Related Accession (Same CDS sequence) XM_025153565.1 , XM_025153571.1 , XM_025153564.1 , XM_025153568.1 , XM_025153569.1 , XM_025153574.1 , XM_025153567.1 , XM_025153573.1 , XM_025153570.1 , XM_025153572.1
    Accession Version XM_025153574.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGC AGAATGTCTC
    CCTGGCAAGT ACGGCCCCAA CTGCAGCTTG GACTGCTCAT GCCAGCACAA CGGCACCTGC
    GACAGGTTCA CGGGCTGCTG CCTCTGCCCC GCGGGGTTCT ACGGGCGCTC CTGTGAGCAC
    GGGTGCCCCC CCGGGTTTTA TGGGCTGAGC TGCCTTCATG CATGTGCCTG TAAAAACGGA
    GCGAGCTGCG ATGCCGTGAT GGGACAGTGC ATTTGTCCCT CGGGGTACCA TGGTATCCAC
    TGTGAGAAAG GCTGTGCCAG TGGGCGCTTC GGTGAGCGGT GCCAGCGCCA GTGCGACTGT
    GGAGGAGCTC CCTGCGACCC GGCCACTGGG AAATGCCTCT GCCCACCTGG AAAGACTGGG
    GACAAGTGTG ACATTGGCTG CCGGTCTGAC CAGTACGGCC CCGACTGCTC TCTGCGATGC
    CAGTGTGCCA GCAAGTCCCA GTGCAACCCA TACAATGGGA AATGTGTCTG TCCAGCCACG
    TGGATGGGGC CAACCTGCAA GGAAGGTGGC CCTCCAAAGC TTCCTTTTTA TGAAGAACAA
    ACTCAAGAAA GAGGGAATAT TTTTCCGAGA GCTCTACAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009342.1
    CDS179..4723
    Translation

    Target ORF information:

    RefSeq Version XM_025153574.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153574.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGC AGAATGTCTC
    CCTGGCAAGT ACGGCCCCAA CTGCAGCTTG GACTGCTCAT GCCAGCACAA CGGCACCTGC
    GACAGGTTCA CGGGCTGCTG CCTCTGCCCC GCGGGGTTCT ACGGGCGCTC CTGTGAGCAC
    GGGTGCCCCC CCGGGTTTTA TGGGCTGAGC TGCCTTCATG CATGTGCCTG TAAAAACGGA
    GCGAGCTGCG ATGCCGTGAT GGGACAGTGC ATTTGTCCCT CGGGGTACCA TGGTATCCAC
    TGTGAGAAAG GCTGTGCCAG TGGGCGCTTC GGTGAGCGGT GCCAGCGCCA GTGCGACTGT
    GGAGGAGCTC CCTGCGACCC GGCCACTGGG AAATGCCTCT GCCCACCTGG AAAGACTGGG
    GACAAGTGTG ACATTGGCTG CCGGTCTGAC CAGTACGGCC CCGACTGCTC TCTGCGATGC
    CAGTGTGCCA GCAAGTCCCA GTGCAACCCA TACAATGGGA AATGTGTCTG TCCAGCCACG
    TGGATGGGGC CAACCTGCAA GGAAGGTGGC CCTCCAAAGC TTCCTTTTTA TGAAGAACAA
    ACTCAAGAAA GAGGGAATAT TTTTCCGAGA GCTCTACAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152774
    Clone ID Related Accession (Same CDS sequence) XM_025153565.1 , XM_025153571.1 , XM_025153564.1 , XM_025153568.1 , XM_025153569.1 , XM_025153574.1 , XM_025153567.1 , XM_025153573.1 , XM_025153570.1 , XM_025153572.1
    Accession Version XM_025153567.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGC AGAATGTCTC
    CCTGGCAAGT ACGGCCCCAA CTGCAGCTTG GACTGCTCAT GCCAGCACAA CGGCACCTGC
    GACAGGTTCA CGGGCTGCTG CCTCTGCCCC GCGGGGTTCT ACGGGCGCTC CTGTGAGCAC
    GGGTGCCCCC CCGGGTTTTA TGGGCTGAGC TGCCTTCATG CATGTGCCTG TAAAAACGGA
    GCGAGCTGCG ATGCCGTGAT GGGACAGTGC ATTTGTCCCT CGGGGTACCA TGGTATCCAC
    TGTGAGAAAG GCTGTGCCAG TGGGCGCTTC GGTGAGCGGT GCCAGCGCCA GTGCGACTGT
    GGAGGAGCTC CCTGCGACCC GGCCACTGGG AAATGCCTCT GCCCACCTGG AAAGACTGGG
    GACAAGTGTG ACATTGGCTG CCGGTCTGAC CAGTACGGCC CCGACTGCTC TCTGCGATGC
    CAGTGTGCCA GCAAGTCCCA GTGCAACCCA TACAATGGGA AATGTGTCTG TCCAGCCACG
    TGGATGGGGC CAACCTGCAA GGAAGGTGGC CCTCCAAAGC TTCCTTTTTA TGAAGAACAA
    ACTCAAGAAA GAGGGAATAT TTTTCCGAGA GCTCTACAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009335.1
    CDS179..4723
    Translation

    Target ORF information:

    RefSeq Version XM_025153567.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153567.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGC AGAATGTCTC
    CCTGGCAAGT ACGGCCCCAA CTGCAGCTTG GACTGCTCAT GCCAGCACAA CGGCACCTGC
    GACAGGTTCA CGGGCTGCTG CCTCTGCCCC GCGGGGTTCT ACGGGCGCTC CTGTGAGCAC
    GGGTGCCCCC CCGGGTTTTA TGGGCTGAGC TGCCTTCATG CATGTGCCTG TAAAAACGGA
    GCGAGCTGCG ATGCCGTGAT GGGACAGTGC ATTTGTCCCT CGGGGTACCA TGGTATCCAC
    TGTGAGAAAG GCTGTGCCAG TGGGCGCTTC GGTGAGCGGT GCCAGCGCCA GTGCGACTGT
    GGAGGAGCTC CCTGCGACCC GGCCACTGGG AAATGCCTCT GCCCACCTGG AAAGACTGGG
    GACAAGTGTG ACATTGGCTG CCGGTCTGAC CAGTACGGCC CCGACTGCTC TCTGCGATGC
    CAGTGTGCCA GCAAGTCCCA GTGCAACCCA TACAATGGGA AATGTGTCTG TCCAGCCACG
    TGGATGGGGC CAACCTGCAA GGAAGGTGGC CCTCCAAAGC TTCCTTTTTA TGAAGAACAA
    ACTCAAGAAA GAGGGAATAT TTTTCCGAGA GCTCTACAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152774
    Clone ID Related Accession (Same CDS sequence) XM_025153565.1 , XM_025153571.1 , XM_025153564.1 , XM_025153568.1 , XM_025153569.1 , XM_025153574.1 , XM_025153567.1 , XM_025153573.1 , XM_025153570.1 , XM_025153572.1
    Accession Version XM_025153573.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGC AGAATGTCTC
    CCTGGCAAGT ACGGCCCCAA CTGCAGCTTG GACTGCTCAT GCCAGCACAA CGGCACCTGC
    GACAGGTTCA CGGGCTGCTG CCTCTGCCCC GCGGGGTTCT ACGGGCGCTC CTGTGAGCAC
    GGGTGCCCCC CCGGGTTTTA TGGGCTGAGC TGCCTTCATG CATGTGCCTG TAAAAACGGA
    GCGAGCTGCG ATGCCGTGAT GGGACAGTGC ATTTGTCCCT CGGGGTACCA TGGTATCCAC
    TGTGAGAAAG GCTGTGCCAG TGGGCGCTTC GGTGAGCGGT GCCAGCGCCA GTGCGACTGT
    GGAGGAGCTC CCTGCGACCC GGCCACTGGG AAATGCCTCT GCCCACCTGG AAAGACTGGG
    GACAAGTGTG ACATTGGCTG CCGGTCTGAC CAGTACGGCC CCGACTGCTC TCTGCGATGC
    CAGTGTGCCA GCAAGTCCCA GTGCAACCCA TACAATGGGA AATGTGTCTG TCCAGCCACG
    TGGATGGGGC CAACCTGCAA GGAAGGTGGC CCTCCAAAGC TTCCTTTTTA TGAAGAACAA
    ACTCAAGAAA GAGGGAATAT TTTTCCGAGA GCTCTACAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009341.1
    CDS179..4723
    Translation

    Target ORF information:

    RefSeq Version XM_025153573.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153573.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGC AGAATGTCTC
    CCTGGCAAGT ACGGCCCCAA CTGCAGCTTG GACTGCTCAT GCCAGCACAA CGGCACCTGC
    GACAGGTTCA CGGGCTGCTG CCTCTGCCCC GCGGGGTTCT ACGGGCGCTC CTGTGAGCAC
    GGGTGCCCCC CCGGGTTTTA TGGGCTGAGC TGCCTTCATG CATGTGCCTG TAAAAACGGA
    GCGAGCTGCG ATGCCGTGAT GGGACAGTGC ATTTGTCCCT CGGGGTACCA TGGTATCCAC
    TGTGAGAAAG GCTGTGCCAG TGGGCGCTTC GGTGAGCGGT GCCAGCGCCA GTGCGACTGT
    GGAGGAGCTC CCTGCGACCC GGCCACTGGG AAATGCCTCT GCCCACCTGG AAAGACTGGG
    GACAAGTGTG ACATTGGCTG CCGGTCTGAC CAGTACGGCC CCGACTGCTC TCTGCGATGC
    CAGTGTGCCA GCAAGTCCCA GTGCAACCCA TACAATGGGA AATGTGTCTG TCCAGCCACG
    TGGATGGGGC CAACCTGCAA GGAAGGTGGC CCTCCAAAGC TTCCTTTTTA TGAAGAACAA
    ACTCAAGAAA GAGGGAATAT TTTTCCGAGA GCTCTACAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152774
    Clone ID Related Accession (Same CDS sequence) XM_025153565.1 , XM_025153571.1 , XM_025153564.1 , XM_025153568.1 , XM_025153569.1 , XM_025153574.1 , XM_025153567.1 , XM_025153573.1 , XM_025153570.1 , XM_025153572.1
    Accession Version XM_025153570.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGC AGAATGTCTC
    CCTGGCAAGT ACGGCCCCAA CTGCAGCTTG GACTGCTCAT GCCAGCACAA CGGCACCTGC
    GACAGGTTCA CGGGCTGCTG CCTCTGCCCC GCGGGGTTCT ACGGGCGCTC CTGTGAGCAC
    GGGTGCCCCC CCGGGTTTTA TGGGCTGAGC TGCCTTCATG CATGTGCCTG TAAAAACGGA
    GCGAGCTGCG ATGCCGTGAT GGGACAGTGC ATTTGTCCCT CGGGGTACCA TGGTATCCAC
    TGTGAGAAAG GCTGTGCCAG TGGGCGCTTC GGTGAGCGGT GCCAGCGCCA GTGCGACTGT
    GGAGGAGCTC CCTGCGACCC GGCCACTGGG AAATGCCTCT GCCCACCTGG AAAGACTGGG
    GACAAGTGTG ACATTGGCTG CCGGTCTGAC CAGTACGGCC CCGACTGCTC TCTGCGATGC
    CAGTGTGCCA GCAAGTCCCA GTGCAACCCA TACAATGGGA AATGTGTCTG TCCAGCCACG
    TGGATGGGGC CAACCTGCAA GGAAGGTGGC CCTCCAAAGC TTCCTTTTTA TGAAGAACAA
    ACTCAAGAAA GAGGGAATAT TTTTCCGAGA GCTCTACAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009338.1
    CDS2474..7018
    Translation

    Target ORF information:

    RefSeq Version XM_025153570.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153570.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGC AGAATGTCTC
    CCTGGCAAGT ACGGCCCCAA CTGCAGCTTG GACTGCTCAT GCCAGCACAA CGGCACCTGC
    GACAGGTTCA CGGGCTGCTG CCTCTGCCCC GCGGGGTTCT ACGGGCGCTC CTGTGAGCAC
    GGGTGCCCCC CCGGGTTTTA TGGGCTGAGC TGCCTTCATG CATGTGCCTG TAAAAACGGA
    GCGAGCTGCG ATGCCGTGAT GGGACAGTGC ATTTGTCCCT CGGGGTACCA TGGTATCCAC
    TGTGAGAAAG GCTGTGCCAG TGGGCGCTTC GGTGAGCGGT GCCAGCGCCA GTGCGACTGT
    GGAGGAGCTC CCTGCGACCC GGCCACTGGG AAATGCCTCT GCCCACCTGG AAAGACTGGG
    GACAAGTGTG ACATTGGCTG CCGGTCTGAC CAGTACGGCC CCGACTGCTC TCTGCGATGC
    CAGTGTGCCA GCAAGTCCCA GTGCAACCCA TACAATGGGA AATGTGTCTG TCCAGCCACG
    TGGATGGGGC CAACCTGCAA GGAAGGTGGC CCTCCAAAGC TTCCTTTTTA TGAAGAACAA
    ACTCAAGAAA GAGGGAATAT TTTTCCGAGA GCTCTACAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152774
    Clone ID Related Accession (Same CDS sequence) XM_025153565.1 , XM_025153571.1 , XM_025153564.1 , XM_025153568.1 , XM_025153569.1 , XM_025153574.1 , XM_025153567.1 , XM_025153573.1 , XM_025153570.1 , XM_025153572.1
    Accession Version XM_025153572.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGC AGAATGTCTC
    CCTGGCAAGT ACGGCCCCAA CTGCAGCTTG GACTGCTCAT GCCAGCACAA CGGCACCTGC
    GACAGGTTCA CGGGCTGCTG CCTCTGCCCC GCGGGGTTCT ACGGGCGCTC CTGTGAGCAC
    GGGTGCCCCC CCGGGTTTTA TGGGCTGAGC TGCCTTCATG CATGTGCCTG TAAAAACGGA
    GCGAGCTGCG ATGCCGTGAT GGGACAGTGC ATTTGTCCCT CGGGGTACCA TGGTATCCAC
    TGTGAGAAAG GCTGTGCCAG TGGGCGCTTC GGTGAGCGGT GCCAGCGCCA GTGCGACTGT
    GGAGGAGCTC CCTGCGACCC GGCCACTGGG AAATGCCTCT GCCCACCTGG AAAGACTGGG
    GACAAGTGTG ACATTGGCTG CCGGTCTGAC CAGTACGGCC CCGACTGCTC TCTGCGATGC
    CAGTGTGCCA GCAAGTCCCA GTGCAACCCA TACAATGGGA AATGTGTCTG TCCAGCCACG
    TGGATGGGGC CAACCTGCAA GGAAGGTGGC CCTCCAAAGC TTCCTTTTTA TGAAGAACAA
    ACTCAAGAAA GAGGGAATAT TTTTCCGAGA GCTCTACAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009340.1
    CDS179..4723
    Translation

    Target ORF information:

    RefSeq Version XM_025153572.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153572.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGC AGAATGTCTC
    CCTGGCAAGT ACGGCCCCAA CTGCAGCTTG GACTGCTCAT GCCAGCACAA CGGCACCTGC
    GACAGGTTCA CGGGCTGCTG CCTCTGCCCC GCGGGGTTCT ACGGGCGCTC CTGTGAGCAC
    GGGTGCCCCC CCGGGTTTTA TGGGCTGAGC TGCCTTCATG CATGTGCCTG TAAAAACGGA
    GCGAGCTGCG ATGCCGTGAT GGGACAGTGC ATTTGTCCCT CGGGGTACCA TGGTATCCAC
    TGTGAGAAAG GCTGTGCCAG TGGGCGCTTC GGTGAGCGGT GCCAGCGCCA GTGCGACTGT
    GGAGGAGCTC CCTGCGACCC GGCCACTGGG AAATGCCTCT GCCCACCTGG AAAGACTGGG
    GACAAGTGTG ACATTGGCTG CCGGTCTGAC CAGTACGGCC CCGACTGCTC TCTGCGATGC
    CAGTGTGCCA GCAAGTCCCA GTGCAACCCA TACAATGGGA AATGTGTCTG TCCAGCCACG
    TGGATGGGGC CAACCTGCAA GGAAGGTGGC CCTCCAAAGC TTCCTTTTTA TGAAGAACAA
    ACTCAAGAAA GAGGGAATAT TTTTCCGAGA GCTCTACAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152775
    Clone ID Related Accession (Same CDS sequence) XM_025153575.1
    Accession Version XM_025153575.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCGGTGTCCA GAAGGTACTT ACGGTTCAAA CTGCCAAAAA
    TCCTGTAAAT GCATGAATGG AGGCCATTGC GACCCAGTGT CGGGAACTTG TGATTGCGCG
    CCTGGTTTCA TTGGAGCCGA CTGTAGTAAA ACTTGCCCAG AAAATCGATA CGGGAAGGAC
    TGCGCCCTGT TCTGTGCATG CGGTAAAGGT CATTGTGACC CACGGACTGG CAGCTGTACC
    TGTCCTCCTG GCCAAATGGG ACCTGGCTGT CAGCAAGTGT GTCCCCAGGG ACGGTTCGGC
    CCCAGCTGCC GCCTGACATG CAGCTGTCAG AACGGTGCTA TCTGTGACCA CGTGGATGGC
    AGCTGCACTT GTGGACTTGG CTGGACAGGA AAATCGTGTG AGAAAGCAGA ATGTCTCCCT
    GGCAAGTACG GCCCCAACTG CAGCTTGGAC TGCTCATGCC AGCACAACGG CACCTGCGAC
    AGGTTCACGG GCTGCTGCCT CTGCCCCGCG GGGTTCTACG GGCGCTCCTG TGAGCACGGG
    TGCCCCCCCG GGTTTTATGG GCTGAGCTGC CTTCATGCAT GTGCCTGTAA AAACGGAGCG
    AGCTGCGATG CCGTGATGGG ACAGTGCATT TGTCCCTCGG GGTACCATGG TATCCACTGT
    GAGAAAGGCT GTGCCAGTGG GCGCTTCGGT GAGCGGTGCC AGCGCCAGTG CGACTGTGGA
    GGAGCTCCCT GCGACCCGGC CACTGGGAAA TGCCTCTGCC CACCTGGAAA GACTGGGGAC
    AAGTGTGACA TTGGCTGCCG GTCTGACCAG TACGGCCCCG ACTGCTCTCT GCGATGCCAG
    TGTGCCAGCA AGTCCCAGTG CAACCCATAC AATGGGAAAT GTGTCTGTCC AGCCACGTGG
    ATGGGGCCAA CCTGCAAGGA AGGTGGCCCT CCAAAGCTTC CTTTTTATGA AGAACAAACT
    CAAGAAAGAG GGAATATTTT TCCGAGAGCT CTACAACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009343.1
    CDS178..4719
    Translation

    Target ORF information:

    RefSeq Version XM_025153575.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153575.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCGGTGTCCA GAAGGTACTT ACGGTTCAAA CTGCCAAAAA
    TCCTGTAAAT GCATGAATGG AGGCCATTGC GACCCAGTGT CGGGAACTTG TGATTGCGCG
    CCTGGTTTCA TTGGAGCCGA CTGTAGTAAA ACTTGCCCAG AAAATCGATA CGGGAAGGAC
    TGCGCCCTGT TCTGTGCATG CGGTAAAGGT CATTGTGACC CACGGACTGG CAGCTGTACC
    TGTCCTCCTG GCCAAATGGG ACCTGGCTGT CAGCAAGTGT GTCCCCAGGG ACGGTTCGGC
    CCCAGCTGCC GCCTGACATG CAGCTGTCAG AACGGTGCTA TCTGTGACCA CGTGGATGGC
    AGCTGCACTT GTGGACTTGG CTGGACAGGA AAATCGTGTG AGAAAGCAGA ATGTCTCCCT
    GGCAAGTACG GCCCCAACTG CAGCTTGGAC TGCTCATGCC AGCACAACGG CACCTGCGAC
    AGGTTCACGG GCTGCTGCCT CTGCCCCGCG GGGTTCTACG GGCGCTCCTG TGAGCACGGG
    TGCCCCCCCG GGTTTTATGG GCTGAGCTGC CTTCATGCAT GTGCCTGTAA AAACGGAGCG
    AGCTGCGATG CCGTGATGGG ACAGTGCATT TGTCCCTCGG GGTACCATGG TATCCACTGT
    GAGAAAGGCT GTGCCAGTGG GCGCTTCGGT GAGCGGTGCC AGCGCCAGTG CGACTGTGGA
    GGAGCTCCCT GCGACCCGGC CACTGGGAAA TGCCTCTGCC CACCTGGAAA GACTGGGGAC
    AAGTGTGACA TTGGCTGCCG GTCTGACCAG TACGGCCCCG ACTGCTCTCT GCGATGCCAG
    TGTGCCAGCA AGTCCCAGTG CAACCCATAC AATGGGAAAT GTGTCTGTCC AGCCACGTGG
    ATGGGGCCAA CCTGCAAGGA AGGTGGCCCT CCAAAGCTTC CTTTTTATGA AGAACAAACT
    CAAGAAAGAG GGAATATTTT TCCGAGAGCT CTACAACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152776
    Clone ID Related Accession (Same CDS sequence) XM_025153577.1
    Accession Version XM_025153577.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4509bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGGGGCT CCGGGGCTGC AGTTCAGAGG TGCCCCCCAG
    CTCCTGCACT ATGTGCTTGG GGCCCCACGT GAGGATGAGG AGCAGCGAGG AGAGCTGACA
    CTGATACACA GAGCCGTTTG CCTTGATAAC ACTTTTGGCA ATGACTGCAG CTTGAGCTGT
    GAGGATTGTA TGAATGGAGG CAGATGTAAC AGTGAGAACA GTGGCTGTGT TTGCTCACCT
    GGGTGGACCG GCATCATCTG CAACCAAACC TGCGCCCAGG GTACATACGG TGATGGTTGT
    GCCAGTATCT GCATGTGCCA GAATGGAGGC TCCTGTGACC CCATCACAGG GCTTTGTCAC
    TGCCCACCAG GAGTCCAGGG GCCATTTTGT GAAGACGGTT GTCCCAGGGG ATTTTTTGGG
    AAGAACTGCA ACAAACCATG CAACTGTGCC AACAGCGGCC ACTGCCACCG GCTATATGGA
    GCCTGCCTGT GTGACCCTGG GCTGTATGGA CGCTTCTGCC ACCTCACCTG TCCCAGGTGG
    GCTTTTGGTG TGGGCTGCTC ACAGGAGTGT CAGTGTGTGA AGGAGCACAC ACTGGGATGC
    AGCGCTCGGA ACGGGACCTG CAGCTGCAAG CCTGGATATC ACGGCAAGAA GTGCCAGAAA
    GCATGCACAC CTGGCTTTTA TGGGGCTGGT TGCAAACTGA GGTGCAACTG TTCCACCGAC
    GTTTTGTGTG ACCACGAGAC GGGAGCCTGC AAGCGTCCGT GCCCGCCTGG TTTCCATGGA
    GAAAAGTGCC ATTCCTCTTG CCAGCCCGGG AGCTTTGGGG TGGACTGCAG GCAGAGCTGC
    AGCTGCGGGG GAGCACCGTG TGACCCAGAG ACTGGGCGTT GTTTCTGCCC AGCTGGGAAA
    ACTGGTGCCA TGTGTGAGAG AGCCTGCCCT GTTGGATACT ACGGACAAAA CTGCCAGCTG
    CCATGCAGCT GCAGCAGTGA TGCTCTGTGT CACCACATCA CTGGGGAGTG CACGTGTCCC
    CCCGGCTGGA CTGGCCACGA CTGCAAGCAC CCCTGCAATA GCGGCCGTTG GGGACAGCAC
    TGTGAAAACT CCTGTGCCTG CAATAACAGC GATGGGAGCT GCGATCCTGT CACTGGGTCC
    TGCTTCTGTG AGCCAGGATT CACTGGGAAA CACTGCGAGC AGAGATGTCC TGAGGGAAGC
    TTTGGTCCCA GCTGCATGCA CAGCTGCCAG TGCCAGAACG GGGCTGCATG TGACCATGTG
    AGCGGAGCCT GCACGTGCAG TGCTGGATGG ACCGGGACCT TCTGCAGGAA AGCTTGTCCA
    GAAGGATTTT TTGGGCTTGA CTGCCACCAG GTGTGCAACT GCAAGAACGC TGAGGGCTGT
    GACCATGTCC TGGGGACGTG CCGCTGCCTC CCAGGCTGGA GGGGAGAGAC CTGCGAGCAG
    CCGTGTCAGG GGGACAGGTT TGGGCAGGGC TGTGCTCACA CCTGCAACTG TCACAACGGA
    GCACTCTGCG ACCACATAAC GGGGACATGT GCCTGCACCC CAGGCTGGGG AGGAGCCACC
    TGCCAAGAAG CCTGTCCCCC AGGCTGGCAT GGTGCTGGCT GCCTTCAGCG CTGCATTTGC
    CCTGGCCAGA CTGCATGTGA CCCCGTGACG GGCAAGTGCC AGTGTTCCAA GGGCGGAACA
    GGGACATCCT GTGAGCTTGC ATGTCCCCCA GGATTTTTTG GAGAGAGATG TGAAGAGCAA
    TGTGACTGCA TTCACAGCGT GTCCTGCCAC CCCCAAACGG GGGCTTGTCG CTGTGCCAAG
    GGGTGGCGAG GCAGGCACTG TGACAAACCC TGCTTGCCTG GCCACTACGG CATGGGTTGT
    GCCCGGCGGT GCCACTGCCC TGCAGGCACC CCCTGCCACC ACCTGACGGG TGAGTGCAGC
    TGTCCTCCAG GATTCACTGG CCATGGCTGC GAGAAAACTT GTCCACCAAA CACGTATGGC
    CAGAGTTGCA GCCAAGCCTG TCGCTGCCGT GCTGAGGAGG ACTGTCACCC CATCACTGGG
    CGGTGCGTGT GCCGCCTGGG GTACCACGGG GCCCAGTGTG ACCGCCCAGG GTGTCCAGAA
    GGTACTTACG GTTCAAACTG CCAAAAATCC TGTAAATGCA TGAATGGAGG CCATTGCGAC
    CCAGTGTCGG GAACTTGTGA TTGCGCGCCT GGTTTCATTG GAGCCGACTG TAGTAAAACT
    TGCCCAGAAA ATCGATACGG GAAGGACTGC GCCCTGTTCT GTGCATGCGG TAAAGGTCAT
    TGTGACCCAC GGACTGGCAG CTGTACCTGT CCTCCTGGCC AAATGGGACC TGGCTGTCAG
    CAAGTGTGTC CCCAGGGACG GTTCGGCCCC AGCTGCCGCC TGACATGCAG CTGTCAGAAC
    GGTGCTATCT GTGACCACGT GGATGGCAGC TGCACTTGTG GACTTGGCTG GACAGGAAAA
    TCGTGTGAGA AAGCAGAATG TCTCCCTGGC AAGTACGGCC CCAACTGCAG CTTGGACTGC
    TCATGCCAGC ACAACGGCAC CTGCGACAGG TTCACGGGCT GCTGCCTCTG CCCCGCGGGG
    TTCTACGGGC GCTCCTGTGA GCACGGGTGC CCCCCCGGGT TTTATGGGCT GAGCTGCCTT
    CATGCATGTG CCTGTAAAAA CGGAGCGAGC TGCGATGCCG TGATGGGACA GTGCATTTGT
    CCCTCGGGGT ACCATGGTAT CCACTGTGAG AAAGGCTGTG CCAGTGGGCG CTTCGGTGAG
    CGGTGCCAGC GCCAGTGCGA CTGTGGAGGA GCTCCCTGCG ACCCGGCCAC TGGGAAATGC
    CTCTGCCCAC CTGGAAAGAC TGGGGACAAG TGTGACATTG GCTGCCGGTC TGACCAGTAC
    GGCCCCGACT GCTCTCTGCG ATGCCAGTGT GCCAGCAAGT CCCAGTGCAA CCCATACAAT
    GGGAAATGTG TCTGTCCAGC CACGTGGATG GGGCCAACCT GCAAGGAAGG TGGCCCTCCA
    AAGCTTCCTT TTTATGAAGA ACAAACTCAA GAAAGAGGGA ATATTTTTCC GAGAGCTCTA
    CAACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009345.1
    CDS179..4687
    Translation

    Target ORF information:

    RefSeq Version XM_025153577.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153577.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGGGGCT CCGGGGCTGC AGTTCAGAGG TGCCCCCCAG
    CTCCTGCACT ATGTGCTTGG GGCCCCACGT GAGGATGAGG AGCAGCGAGG AGAGCTGACA
    CTGATACACA GAGCCGTTTG CCTTGATAAC ACTTTTGGCA ATGACTGCAG CTTGAGCTGT
    GAGGATTGTA TGAATGGAGG CAGATGTAAC AGTGAGAACA GTGGCTGTGT TTGCTCACCT
    GGGTGGACCG GCATCATCTG CAACCAAACC TGCGCCCAGG GTACATACGG TGATGGTTGT
    GCCAGTATCT GCATGTGCCA GAATGGAGGC TCCTGTGACC CCATCACAGG GCTTTGTCAC
    TGCCCACCAG GAGTCCAGGG GCCATTTTGT GAAGACGGTT GTCCCAGGGG ATTTTTTGGG
    AAGAACTGCA ACAAACCATG CAACTGTGCC AACAGCGGCC ACTGCCACCG GCTATATGGA
    GCCTGCCTGT GTGACCCTGG GCTGTATGGA CGCTTCTGCC ACCTCACCTG TCCCAGGTGG
    GCTTTTGGTG TGGGCTGCTC ACAGGAGTGT CAGTGTGTGA AGGAGCACAC ACTGGGATGC
    AGCGCTCGGA ACGGGACCTG CAGCTGCAAG CCTGGATATC ACGGCAAGAA GTGCCAGAAA
    GCATGCACAC CTGGCTTTTA TGGGGCTGGT TGCAAACTGA GGTGCAACTG TTCCACCGAC
    GTTTTGTGTG ACCACGAGAC GGGAGCCTGC AAGCGTCCGT GCCCGCCTGG TTTCCATGGA
    GAAAAGTGCC ATTCCTCTTG CCAGCCCGGG AGCTTTGGGG TGGACTGCAG GCAGAGCTGC
    AGCTGCGGGG GAGCACCGTG TGACCCAGAG ACTGGGCGTT GTTTCTGCCC AGCTGGGAAA
    ACTGGTGCCA TGTGTGAGAG AGCCTGCCCT GTTGGATACT ACGGACAAAA CTGCCAGCTG
    CCATGCAGCT GCAGCAGTGA TGCTCTGTGT CACCACATCA CTGGGGAGTG CACGTGTCCC
    CCCGGCTGGA CTGGCCACGA CTGCAAGCAC CCCTGCAATA GCGGCCGTTG GGGACAGCAC
    TGTGAAAACT CCTGTGCCTG CAATAACAGC GATGGGAGCT GCGATCCTGT CACTGGGTCC
    TGCTTCTGTG AGCCAGGATT CACTGGGAAA CACTGCGAGC AGAGATGTCC TGAGGGAAGC
    TTTGGTCCCA GCTGCATGCA CAGCTGCCAG TGCCAGAACG GGGCTGCATG TGACCATGTG
    AGCGGAGCCT GCACGTGCAG TGCTGGATGG ACCGGGACCT TCTGCAGGAA AGCTTGTCCA
    GAAGGATTTT TTGGGCTTGA CTGCCACCAG GTGTGCAACT GCAAGAACGC TGAGGGCTGT
    GACCATGTCC TGGGGACGTG CCGCTGCCTC CCAGGCTGGA GGGGAGAGAC CTGCGAGCAG
    CCGTGTCAGG GGGACAGGTT TGGGCAGGGC TGTGCTCACA CCTGCAACTG TCACAACGGA
    GCACTCTGCG ACCACATAAC GGGGACATGT GCCTGCACCC CAGGCTGGGG AGGAGCCACC
    TGCCAAGAAG CCTGTCCCCC AGGCTGGCAT GGTGCTGGCT GCCTTCAGCG CTGCATTTGC
    CCTGGCCAGA CTGCATGTGA CCCCGTGACG GGCAAGTGCC AGTGTTCCAA GGGCGGAACA
    GGGACATCCT GTGAGCTTGC ATGTCCCCCA GGATTTTTTG GAGAGAGATG TGAAGAGCAA
    TGTGACTGCA TTCACAGCGT GTCCTGCCAC CCCCAAACGG GGGCTTGTCG CTGTGCCAAG
    GGGTGGCGAG GCAGGCACTG TGACAAACCC TGCTTGCCTG GCCACTACGG CATGGGTTGT
    GCCCGGCGGT GCCACTGCCC TGCAGGCACC CCCTGCCACC ACCTGACGGG TGAGTGCAGC
    TGTCCTCCAG GATTCACTGG CCATGGCTGC GAGAAAACTT GTCCACCAAA CACGTATGGC
    CAGAGTTGCA GCCAAGCCTG TCGCTGCCGT GCTGAGGAGG ACTGTCACCC CATCACTGGG
    CGGTGCGTGT GCCGCCTGGG GTACCACGGG GCCCAGTGTG ACCGCCCAGG GTGTCCAGAA
    GGTACTTACG GTTCAAACTG CCAAAAATCC TGTAAATGCA TGAATGGAGG CCATTGCGAC
    CCAGTGTCGG GAACTTGTGA TTGCGCGCCT GGTTTCATTG GAGCCGACTG TAGTAAAACT
    TGCCCAGAAA ATCGATACGG GAAGGACTGC GCCCTGTTCT GTGCATGCGG TAAAGGTCAT
    TGTGACCCAC GGACTGGCAG CTGTACCTGT CCTCCTGGCC AAATGGGACC TGGCTGTCAG
    CAAGTGTGTC CCCAGGGACG GTTCGGCCCC AGCTGCCGCC TGACATGCAG CTGTCAGAAC
    GGTGCTATCT GTGACCACGT GGATGGCAGC TGCACTTGTG GACTTGGCTG GACAGGAAAA
    TCGTGTGAGA AAGCAGAATG TCTCCCTGGC AAGTACGGCC CCAACTGCAG CTTGGACTGC
    TCATGCCAGC ACAACGGCAC CTGCGACAGG TTCACGGGCT GCTGCCTCTG CCCCGCGGGG
    TTCTACGGGC GCTCCTGTGA GCACGGGTGC CCCCCCGGGT TTTATGGGCT GAGCTGCCTT
    CATGCATGTG CCTGTAAAAA CGGAGCGAGC TGCGATGCCG TGATGGGACA GTGCATTTGT
    CCCTCGGGGT ACCATGGTAT CCACTGTGAG AAAGGCTGTG CCAGTGGGCG CTTCGGTGAG
    CGGTGCCAGC GCCAGTGCGA CTGTGGAGGA GCTCCCTGCG ACCCGGCCAC TGGGAAATGC
    CTCTGCCCAC CTGGAAAGAC TGGGGACAAG TGTGACATTG GCTGCCGGTC TGACCAGTAC
    GGCCCCGACT GCTCTCTGCG ATGCCAGTGT GCCAGCAAGT CCCAGTGCAA CCCATACAAT
    GGGAAATGTG TCTGTCCAGC CACGTGGATG GGGCCAACCT GCAAGGAAGG TGGCCCTCCA
    AAGCTTCCTT TTTATGAAGA ACAAACTCAA GAAAGAGGGA ATATTTTTCC GAGAGCTCTA
    CAACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40120
    Clone ID Related Accession (Same CDS sequence) XM_015291738.1 , XM_015291739.1 , XM_015291740.1 , XM_015291734.1 , XM_015291735.1 , XM_015291741.1 , XM_015291742.1 , XM_015291737.2 , XM_015291733.1 , XM_015291737.1
    Accession Version XM_015291738.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGA ATGTCTCCCT
    GGCAAGTACG GCCCCAACTG CAGCTTGGAC TGCTCATGCC AGCACAACGG CACCTGCGAC
    AGGTTCACGG GCTGCTGCCT CTGCCCCGCG GGGTTCTACG GGCGCTCCTG TGAGCACGGG
    TGCCCCCCCG GGTTTTATGG GCTGAGCTGC CTTCATGCAT GTGCCTGTAA AAACGGAGCG
    AGCTGCGATG CCGTGATGGG ACAGTGCATT TGTCCCTCGG GGTACCATGG TATCCACTGT
    GAGAAAGGCT GTGCCAGTGG GCGCTTCGGT GAGCGGTGCC AGCGCCAGTG CGACTGTGGA
    GGAGCTCCCT GCGACCCGGC CACTGGGAAA TGCCTCTGCC CACCTGGAAA GACTGGGGAC
    AAGTGTGACA TTGGCTGCCG GTCTGACCAG TACGGCCCCG ACTGCTCTCT GCGATGCCAG
    TGTGCCAGCA AGTCCCAGTG CAACCCATAC AATGGGAAAT GTGTCTGTCC AGCCACGTGG
    ATGGGGCCAA CCTGCAAGGA AGGTGGCCCT CCAAAGCTTC CTTTTTATGA AGAACAAACT
    CAAGAAAGAG GGAATATTTT TCCGAGAGCT CTACAACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147224.1
    CDS176..4717
    Translation

    Target ORF information:

    RefSeq Version XM_015291738.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291738.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGA ATGTCTCCCT
    GGCAAGTACG GCCCCAACTG CAGCTTGGAC TGCTCATGCC AGCACAACGG CACCTGCGAC
    AGGTTCACGG GCTGCTGCCT CTGCCCCGCG GGGTTCTACG GGCGCTCCTG TGAGCACGGG
    TGCCCCCCCG GGTTTTATGG GCTGAGCTGC CTTCATGCAT GTGCCTGTAA AAACGGAGCG
    AGCTGCGATG CCGTGATGGG ACAGTGCATT TGTCCCTCGG GGTACCATGG TATCCACTGT
    GAGAAAGGCT GTGCCAGTGG GCGCTTCGGT GAGCGGTGCC AGCGCCAGTG CGACTGTGGA
    GGAGCTCCCT GCGACCCGGC CACTGGGAAA TGCCTCTGCC CACCTGGAAA GACTGGGGAC
    AAGTGTGACA TTGGCTGCCG GTCTGACCAG TACGGCCCCG ACTGCTCTCT GCGATGCCAG
    TGTGCCAGCA AGTCCCAGTG CAACCCATAC AATGGGAAAT GTGTCTGTCC AGCCACGTGG
    ATGGGGCCAA CCTGCAAGGA AGGTGGCCCT CCAAAGCTTC CTTTTTATGA AGAACAAACT
    CAAGAAAGAG GGAATATTTT TCCGAGAGCT CTACAACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40120
    Clone ID Related Accession (Same CDS sequence) XM_015291738.1 , XM_015291739.1 , XM_015291740.1 , XM_015291734.1 , XM_015291735.1 , XM_015291741.1 , XM_015291742.1 , XM_015291737.2 , XM_015291733.1 , XM_015291737.1
    Accession Version XM_015291739.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGA ATGTCTCCCT
    GGCAAGTACG GCCCCAACTG CAGCTTGGAC TGCTCATGCC AGCACAACGG CACCTGCGAC
    AGGTTCACGG GCTGCTGCCT CTGCCCCGCG GGGTTCTACG GGCGCTCCTG TGAGCACGGG
    TGCCCCCCCG GGTTTTATGG GCTGAGCTGC CTTCATGCAT GTGCCTGTAA AAACGGAGCG
    AGCTGCGATG CCGTGATGGG ACAGTGCATT TGTCCCTCGG GGTACCATGG TATCCACTGT
    GAGAAAGGCT GTGCCAGTGG GCGCTTCGGT GAGCGGTGCC AGCGCCAGTG CGACTGTGGA
    GGAGCTCCCT GCGACCCGGC CACTGGGAAA TGCCTCTGCC CACCTGGAAA GACTGGGGAC
    AAGTGTGACA TTGGCTGCCG GTCTGACCAG TACGGCCCCG ACTGCTCTCT GCGATGCCAG
    TGTGCCAGCA AGTCCCAGTG CAACCCATAC AATGGGAAAT GTGTCTGTCC AGCCACGTGG
    ATGGGGCCAA CCTGCAAGGA AGGTGGCCCT CCAAAGCTTC CTTTTTATGA AGAACAAACT
    CAAGAAAGAG GGAATATTTT TCCGAGAGCT CTACAACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147225.1
    CDS176..4717
    Translation

    Target ORF information:

    RefSeq Version XM_015291739.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291739.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGA ATGTCTCCCT
    GGCAAGTACG GCCCCAACTG CAGCTTGGAC TGCTCATGCC AGCACAACGG CACCTGCGAC
    AGGTTCACGG GCTGCTGCCT CTGCCCCGCG GGGTTCTACG GGCGCTCCTG TGAGCACGGG
    TGCCCCCCCG GGTTTTATGG GCTGAGCTGC CTTCATGCAT GTGCCTGTAA AAACGGAGCG
    AGCTGCGATG CCGTGATGGG ACAGTGCATT TGTCCCTCGG GGTACCATGG TATCCACTGT
    GAGAAAGGCT GTGCCAGTGG GCGCTTCGGT GAGCGGTGCC AGCGCCAGTG CGACTGTGGA
    GGAGCTCCCT GCGACCCGGC CACTGGGAAA TGCCTCTGCC CACCTGGAAA GACTGGGGAC
    AAGTGTGACA TTGGCTGCCG GTCTGACCAG TACGGCCCCG ACTGCTCTCT GCGATGCCAG
    TGTGCCAGCA AGTCCCAGTG CAACCCATAC AATGGGAAAT GTGTCTGTCC AGCCACGTGG
    ATGGGGCCAA CCTGCAAGGA AGGTGGCCCT CCAAAGCTTC CTTTTTATGA AGAACAAACT
    CAAGAAAGAG GGAATATTTT TCCGAGAGCT CTACAACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40120
    Clone ID Related Accession (Same CDS sequence) XM_015291738.1 , XM_015291739.1 , XM_015291740.1 , XM_015291734.1 , XM_015291735.1 , XM_015291741.1 , XM_015291742.1 , XM_015291737.2 , XM_015291733.1 , XM_015291737.1
    Accession Version XM_015291740.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGA ATGTCTCCCT
    GGCAAGTACG GCCCCAACTG CAGCTTGGAC TGCTCATGCC AGCACAACGG CACCTGCGAC
    AGGTTCACGG GCTGCTGCCT CTGCCCCGCG GGGTTCTACG GGCGCTCCTG TGAGCACGGG
    TGCCCCCCCG GGTTTTATGG GCTGAGCTGC CTTCATGCAT GTGCCTGTAA AAACGGAGCG
    AGCTGCGATG CCGTGATGGG ACAGTGCATT TGTCCCTCGG GGTACCATGG TATCCACTGT
    GAGAAAGGCT GTGCCAGTGG GCGCTTCGGT GAGCGGTGCC AGCGCCAGTG CGACTGTGGA
    GGAGCTCCCT GCGACCCGGC CACTGGGAAA TGCCTCTGCC CACCTGGAAA GACTGGGGAC
    AAGTGTGACA TTGGCTGCCG GTCTGACCAG TACGGCCCCG ACTGCTCTCT GCGATGCCAG
    TGTGCCAGCA AGTCCCAGTG CAACCCATAC AATGGGAAAT GTGTCTGTCC AGCCACGTGG
    ATGGGGCCAA CCTGCAAGGA AGGTGGCCCT CCAAAGCTTC CTTTTTATGA AGAACAAACT
    CAAGAAAGAG GGAATATTTT TCCGAGAGCT CTACAACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147226.1
    CDS176..4717
    Translation

    Target ORF information:

    RefSeq Version XM_015291740.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291740.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGA ATGTCTCCCT
    GGCAAGTACG GCCCCAACTG CAGCTTGGAC TGCTCATGCC AGCACAACGG CACCTGCGAC
    AGGTTCACGG GCTGCTGCCT CTGCCCCGCG GGGTTCTACG GGCGCTCCTG TGAGCACGGG
    TGCCCCCCCG GGTTTTATGG GCTGAGCTGC CTTCATGCAT GTGCCTGTAA AAACGGAGCG
    AGCTGCGATG CCGTGATGGG ACAGTGCATT TGTCCCTCGG GGTACCATGG TATCCACTGT
    GAGAAAGGCT GTGCCAGTGG GCGCTTCGGT GAGCGGTGCC AGCGCCAGTG CGACTGTGGA
    GGAGCTCCCT GCGACCCGGC CACTGGGAAA TGCCTCTGCC CACCTGGAAA GACTGGGGAC
    AAGTGTGACA TTGGCTGCCG GTCTGACCAG TACGGCCCCG ACTGCTCTCT GCGATGCCAG
    TGTGCCAGCA AGTCCCAGTG CAACCCATAC AATGGGAAAT GTGTCTGTCC AGCCACGTGG
    ATGGGGCCAA CCTGCAAGGA AGGTGGCCCT CCAAAGCTTC CTTTTTATGA AGAACAAACT
    CAAGAAAGAG GGAATATTTT TCCGAGAGCT CTACAACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40120
    Clone ID Related Accession (Same CDS sequence) XM_015291738.1 , XM_015291739.1 , XM_015291740.1 , XM_015291734.1 , XM_015291735.1 , XM_015291741.1 , XM_015291742.1 , XM_015291737.2 , XM_015291733.1 , XM_015291737.1
    Accession Version XM_015291734.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGA ATGTCTCCCT
    GGCAAGTACG GCCCCAACTG CAGCTTGGAC TGCTCATGCC AGCACAACGG CACCTGCGAC
    AGGTTCACGG GCTGCTGCCT CTGCCCCGCG GGGTTCTACG GGCGCTCCTG TGAGCACGGG
    TGCCCCCCCG GGTTTTATGG GCTGAGCTGC CTTCATGCAT GTGCCTGTAA AAACGGAGCG
    AGCTGCGATG CCGTGATGGG ACAGTGCATT TGTCCCTCGG GGTACCATGG TATCCACTGT
    GAGAAAGGCT GTGCCAGTGG GCGCTTCGGT GAGCGGTGCC AGCGCCAGTG CGACTGTGGA
    GGAGCTCCCT GCGACCCGGC CACTGGGAAA TGCCTCTGCC CACCTGGAAA GACTGGGGAC
    AAGTGTGACA TTGGCTGCCG GTCTGACCAG TACGGCCCCG ACTGCTCTCT GCGATGCCAG
    TGTGCCAGCA AGTCCCAGTG CAACCCATAC AATGGGAAAT GTGTCTGTCC AGCCACGTGG
    ATGGGGCCAA CCTGCAAGGA AGGTGGCCCT CCAAAGCTTC CTTTTTATGA AGAACAAACT
    CAAGAAAGAG GGAATATTTT TCCGAGAGCT CTACAACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147220.1
    CDS176..4717
    Translation

    Target ORF information:

    RefSeq Version XM_015291734.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291734.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGA ATGTCTCCCT
    GGCAAGTACG GCCCCAACTG CAGCTTGGAC TGCTCATGCC AGCACAACGG CACCTGCGAC
    AGGTTCACGG GCTGCTGCCT CTGCCCCGCG GGGTTCTACG GGCGCTCCTG TGAGCACGGG
    TGCCCCCCCG GGTTTTATGG GCTGAGCTGC CTTCATGCAT GTGCCTGTAA AAACGGAGCG
    AGCTGCGATG CCGTGATGGG ACAGTGCATT TGTCCCTCGG GGTACCATGG TATCCACTGT
    GAGAAAGGCT GTGCCAGTGG GCGCTTCGGT GAGCGGTGCC AGCGCCAGTG CGACTGTGGA
    GGAGCTCCCT GCGACCCGGC CACTGGGAAA TGCCTCTGCC CACCTGGAAA GACTGGGGAC
    AAGTGTGACA TTGGCTGCCG GTCTGACCAG TACGGCCCCG ACTGCTCTCT GCGATGCCAG
    TGTGCCAGCA AGTCCCAGTG CAACCCATAC AATGGGAAAT GTGTCTGTCC AGCCACGTGG
    ATGGGGCCAA CCTGCAAGGA AGGTGGCCCT CCAAAGCTTC CTTTTTATGA AGAACAAACT
    CAAGAAAGAG GGAATATTTT TCCGAGAGCT CTACAACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40120
    Clone ID Related Accession (Same CDS sequence) XM_015291738.1 , XM_015291739.1 , XM_015291740.1 , XM_015291734.1 , XM_015291735.1 , XM_015291741.1 , XM_015291742.1 , XM_015291737.2 , XM_015291733.1 , XM_015291737.1
    Accession Version XM_015291735.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGA ATGTCTCCCT
    GGCAAGTACG GCCCCAACTG CAGCTTGGAC TGCTCATGCC AGCACAACGG CACCTGCGAC
    AGGTTCACGG GCTGCTGCCT CTGCCCCGCG GGGTTCTACG GGCGCTCCTG TGAGCACGGG
    TGCCCCCCCG GGTTTTATGG GCTGAGCTGC CTTCATGCAT GTGCCTGTAA AAACGGAGCG
    AGCTGCGATG CCGTGATGGG ACAGTGCATT TGTCCCTCGG GGTACCATGG TATCCACTGT
    GAGAAAGGCT GTGCCAGTGG GCGCTTCGGT GAGCGGTGCC AGCGCCAGTG CGACTGTGGA
    GGAGCTCCCT GCGACCCGGC CACTGGGAAA TGCCTCTGCC CACCTGGAAA GACTGGGGAC
    AAGTGTGACA TTGGCTGCCG GTCTGACCAG TACGGCCCCG ACTGCTCTCT GCGATGCCAG
    TGTGCCAGCA AGTCCCAGTG CAACCCATAC AATGGGAAAT GTGTCTGTCC AGCCACGTGG
    ATGGGGCCAA CCTGCAAGGA AGGTGGCCCT CCAAAGCTTC CTTTTTATGA AGAACAAACT
    CAAGAAAGAG GGAATATTTT TCCGAGAGCT CTACAACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147221.1
    CDS176..4717
    Translation

    Target ORF information:

    RefSeq Version XM_015291735.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291735.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGA ATGTCTCCCT
    GGCAAGTACG GCCCCAACTG CAGCTTGGAC TGCTCATGCC AGCACAACGG CACCTGCGAC
    AGGTTCACGG GCTGCTGCCT CTGCCCCGCG GGGTTCTACG GGCGCTCCTG TGAGCACGGG
    TGCCCCCCCG GGTTTTATGG GCTGAGCTGC CTTCATGCAT GTGCCTGTAA AAACGGAGCG
    AGCTGCGATG CCGTGATGGG ACAGTGCATT TGTCCCTCGG GGTACCATGG TATCCACTGT
    GAGAAAGGCT GTGCCAGTGG GCGCTTCGGT GAGCGGTGCC AGCGCCAGTG CGACTGTGGA
    GGAGCTCCCT GCGACCCGGC CACTGGGAAA TGCCTCTGCC CACCTGGAAA GACTGGGGAC
    AAGTGTGACA TTGGCTGCCG GTCTGACCAG TACGGCCCCG ACTGCTCTCT GCGATGCCAG
    TGTGCCAGCA AGTCCCAGTG CAACCCATAC AATGGGAAAT GTGTCTGTCC AGCCACGTGG
    ATGGGGCCAA CCTGCAAGGA AGGTGGCCCT CCAAAGCTTC CTTTTTATGA AGAACAAACT
    CAAGAAAGAG GGAATATTTT TCCGAGAGCT CTACAACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40120
    Clone ID Related Accession (Same CDS sequence) XM_015291738.1 , XM_015291739.1 , XM_015291740.1 , XM_015291734.1 , XM_015291735.1 , XM_015291741.1 , XM_015291742.1 , XM_015291737.2 , XM_015291733.1 , XM_015291737.1
    Accession Version XM_015291741.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGA ATGTCTCCCT
    GGCAAGTACG GCCCCAACTG CAGCTTGGAC TGCTCATGCC AGCACAACGG CACCTGCGAC
    AGGTTCACGG GCTGCTGCCT CTGCCCCGCG GGGTTCTACG GGCGCTCCTG TGAGCACGGG
    TGCCCCCCCG GGTTTTATGG GCTGAGCTGC CTTCATGCAT GTGCCTGTAA AAACGGAGCG
    AGCTGCGATG CCGTGATGGG ACAGTGCATT TGTCCCTCGG GGTACCATGG TATCCACTGT
    GAGAAAGGCT GTGCCAGTGG GCGCTTCGGT GAGCGGTGCC AGCGCCAGTG CGACTGTGGA
    GGAGCTCCCT GCGACCCGGC CACTGGGAAA TGCCTCTGCC CACCTGGAAA GACTGGGGAC
    AAGTGTGACA TTGGCTGCCG GTCTGACCAG TACGGCCCCG ACTGCTCTCT GCGATGCCAG
    TGTGCCAGCA AGTCCCAGTG CAACCCATAC AATGGGAAAT GTGTCTGTCC AGCCACGTGG
    ATGGGGCCAA CCTGCAAGGA AGGTGGCCCT CCAAAGCTTC CTTTTTATGA AGAACAAACT
    CAAGAAAGAG GGAATATTTT TCCGAGAGCT CTACAACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147227.1
    CDS176..4717
    Translation

    Target ORF information:

    RefSeq Version XM_015291741.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291741.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGA ATGTCTCCCT
    GGCAAGTACG GCCCCAACTG CAGCTTGGAC TGCTCATGCC AGCACAACGG CACCTGCGAC
    AGGTTCACGG GCTGCTGCCT CTGCCCCGCG GGGTTCTACG GGCGCTCCTG TGAGCACGGG
    TGCCCCCCCG GGTTTTATGG GCTGAGCTGC CTTCATGCAT GTGCCTGTAA AAACGGAGCG
    AGCTGCGATG CCGTGATGGG ACAGTGCATT TGTCCCTCGG GGTACCATGG TATCCACTGT
    GAGAAAGGCT GTGCCAGTGG GCGCTTCGGT GAGCGGTGCC AGCGCCAGTG CGACTGTGGA
    GGAGCTCCCT GCGACCCGGC CACTGGGAAA TGCCTCTGCC CACCTGGAAA GACTGGGGAC
    AAGTGTGACA TTGGCTGCCG GTCTGACCAG TACGGCCCCG ACTGCTCTCT GCGATGCCAG
    TGTGCCAGCA AGTCCCAGTG CAACCCATAC AATGGGAAAT GTGTCTGTCC AGCCACGTGG
    ATGGGGCCAA CCTGCAAGGA AGGTGGCCCT CCAAAGCTTC CTTTTTATGA AGAACAAACT
    CAAGAAAGAG GGAATATTTT TCCGAGAGCT CTACAACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40120
    Clone ID Related Accession (Same CDS sequence) XM_015291738.1 , XM_015291739.1 , XM_015291740.1 , XM_015291734.1 , XM_015291735.1 , XM_015291741.1 , XM_015291742.1 , XM_015291737.2 , XM_015291733.1 , XM_015291737.1
    Accession Version XM_015291742.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGA ATGTCTCCCT
    GGCAAGTACG GCCCCAACTG CAGCTTGGAC TGCTCATGCC AGCACAACGG CACCTGCGAC
    AGGTTCACGG GCTGCTGCCT CTGCCCCGCG GGGTTCTACG GGCGCTCCTG TGAGCACGGG
    TGCCCCCCCG GGTTTTATGG GCTGAGCTGC CTTCATGCAT GTGCCTGTAA AAACGGAGCG
    AGCTGCGATG CCGTGATGGG ACAGTGCATT TGTCCCTCGG GGTACCATGG TATCCACTGT
    GAGAAAGGCT GTGCCAGTGG GCGCTTCGGT GAGCGGTGCC AGCGCCAGTG CGACTGTGGA
    GGAGCTCCCT GCGACCCGGC CACTGGGAAA TGCCTCTGCC CACCTGGAAA GACTGGGGAC
    AAGTGTGACA TTGGCTGCCG GTCTGACCAG TACGGCCCCG ACTGCTCTCT GCGATGCCAG
    TGTGCCAGCA AGTCCCAGTG CAACCCATAC AATGGGAAAT GTGTCTGTCC AGCCACGTGG
    ATGGGGCCAA CCTGCAAGGA AGGTGGCCCT CCAAAGCTTC CTTTTTATGA AGAACAAACT
    CAAGAAAGAG GGAATATTTT TCCGAGAGCT CTACAACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147228.1
    CDS176..4717
    Translation

    Target ORF information:

    RefSeq Version XM_015291742.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291742.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGA ATGTCTCCCT
    GGCAAGTACG GCCCCAACTG CAGCTTGGAC TGCTCATGCC AGCACAACGG CACCTGCGAC
    AGGTTCACGG GCTGCTGCCT CTGCCCCGCG GGGTTCTACG GGCGCTCCTG TGAGCACGGG
    TGCCCCCCCG GGTTTTATGG GCTGAGCTGC CTTCATGCAT GTGCCTGTAA AAACGGAGCG
    AGCTGCGATG CCGTGATGGG ACAGTGCATT TGTCCCTCGG GGTACCATGG TATCCACTGT
    GAGAAAGGCT GTGCCAGTGG GCGCTTCGGT GAGCGGTGCC AGCGCCAGTG CGACTGTGGA
    GGAGCTCCCT GCGACCCGGC CACTGGGAAA TGCCTCTGCC CACCTGGAAA GACTGGGGAC
    AAGTGTGACA TTGGCTGCCG GTCTGACCAG TACGGCCCCG ACTGCTCTCT GCGATGCCAG
    TGTGCCAGCA AGTCCCAGTG CAACCCATAC AATGGGAAAT GTGTCTGTCC AGCCACGTGG
    ATGGGGCCAA CCTGCAAGGA AGGTGGCCCT CCAAAGCTTC CTTTTTATGA AGAACAAACT
    CAAGAAAGAG GGAATATTTT TCCGAGAGCT CTACAACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40120
    Clone ID Related Accession (Same CDS sequence) XM_015291738.1 , XM_015291739.1 , XM_015291740.1 , XM_015291734.1 , XM_015291735.1 , XM_015291741.1 , XM_015291742.1 , XM_015291737.2 , XM_015291733.1 , XM_015291737.1
    Accession Version XM_015291737.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291737.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGA ATGTCTCCCT
    GGCAAGTACG GCCCCAACTG CAGCTTGGAC TGCTCATGCC AGCACAACGG CACCTGCGAC
    AGGTTCACGG GCTGCTGCCT CTGCCCCGCG GGGTTCTACG GGCGCTCCTG TGAGCACGGG
    TGCCCCCCCG GGTTTTATGG GCTGAGCTGC CTTCATGCAT GTGCCTGTAA AAACGGAGCG
    AGCTGCGATG CCGTGATGGG ACAGTGCATT TGTCCCTCGG GGTACCATGG TATCCACTGT
    GAGAAAGGCT GTGCCAGTGG GCGCTTCGGT GAGCGGTGCC AGCGCCAGTG CGACTGTGGA
    GGAGCTCCCT GCGACCCGGC CACTGGGAAA TGCCTCTGCC CACCTGGAAA GACTGGGGAC
    AAGTGTGACA TTGGCTGCCG GTCTGACCAG TACGGCCCCG ACTGCTCTCT GCGATGCCAG
    TGTGCCAGCA AGTCCCAGTG CAACCCATAC AATGGGAAAT GTGTCTGTCC AGCCACGTGG
    ATGGGGCCAA CCTGCAAGGA AGGTGGCCCT CCAAAGCTTC CTTTTTATGA AGAACAAACT
    CAAGAAAGAG GGAATATTTT TCCGAGAGCT CTACAACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147223.1
    CDS177..4718
    Translation

    Target ORF information:

    RefSeq Version XM_015291737.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (MEGF6L), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291737.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGA ATGTCTCCCT
    GGCAAGTACG GCCCCAACTG CAGCTTGGAC TGCTCATGCC AGCACAACGG CACCTGCGAC
    AGGTTCACGG GCTGCTGCCT CTGCCCCGCG GGGTTCTACG GGCGCTCCTG TGAGCACGGG
    TGCCCCCCCG GGTTTTATGG GCTGAGCTGC CTTCATGCAT GTGCCTGTAA AAACGGAGCG
    AGCTGCGATG CCGTGATGGG ACAGTGCATT TGTCCCTCGG GGTACCATGG TATCCACTGT
    GAGAAAGGCT GTGCCAGTGG GCGCTTCGGT GAGCGGTGCC AGCGCCAGTG CGACTGTGGA
    GGAGCTCCCT GCGACCCGGC CACTGGGAAA TGCCTCTGCC CACCTGGAAA GACTGGGGAC
    AAGTGTGACA TTGGCTGCCG GTCTGACCAG TACGGCCCCG ACTGCTCTCT GCGATGCCAG
    TGTGCCAGCA AGTCCCAGTG CAACCCATAC AATGGGAAAT GTGTCTGTCC AGCCACGTGG
    ATGGGGCCAA CCTGCAAGGA AGGTGGCCCT CCAAAGCTTC CTTTTTATGA AGAACAAACT
    CAAGAAAGAG GGAATATTTT TCCGAGAGCT CTACAACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40120
    Clone ID Related Accession (Same CDS sequence) XM_015291738.1 , XM_015291739.1 , XM_015291740.1 , XM_015291734.1 , XM_015291735.1 , XM_015291741.1 , XM_015291742.1 , XM_015291737.2 , XM_015291733.1 , XM_015291737.1
    Accession Version XM_015291733.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGA ATGTCTCCCT
    GGCAAGTACG GCCCCAACTG CAGCTTGGAC TGCTCATGCC AGCACAACGG CACCTGCGAC
    AGGTTCACGG GCTGCTGCCT CTGCCCCGCG GGGTTCTACG GGCGCTCCTG TGAGCACGGG
    TGCCCCCCCG GGTTTTATGG GCTGAGCTGC CTTCATGCAT GTGCCTGTAA AAACGGAGCG
    AGCTGCGATG CCGTGATGGG ACAGTGCATT TGTCCCTCGG GGTACCATGG TATCCACTGT
    GAGAAAGGCT GTGCCAGTGG GCGCTTCGGT GAGCGGTGCC AGCGCCAGTG CGACTGTGGA
    GGAGCTCCCT GCGACCCGGC CACTGGGAAA TGCCTCTGCC CACCTGGAAA GACTGGGGAC
    AAGTGTGACA TTGGCTGCCG GTCTGACCAG TACGGCCCCG ACTGCTCTCT GCGATGCCAG
    TGTGCCAGCA AGTCCCAGTG CAACCCATAC AATGGGAAAT GTGTCTGTCC AGCCACGTGG
    ATGGGGCCAA CCTGCAAGGA AGGTGGCCCT CCAAAGCTTC CTTTTTATGA AGAACAAACT
    CAAGAAAGAG GGAATATTTT TCCGAGAGCT CTACAACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147219.1
    CDS176..4717
    Translation

    Target ORF information:

    RefSeq Version XM_015291733.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291733.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGA ATGTCTCCCT
    GGCAAGTACG GCCCCAACTG CAGCTTGGAC TGCTCATGCC AGCACAACGG CACCTGCGAC
    AGGTTCACGG GCTGCTGCCT CTGCCCCGCG GGGTTCTACG GGCGCTCCTG TGAGCACGGG
    TGCCCCCCCG GGTTTTATGG GCTGAGCTGC CTTCATGCAT GTGCCTGTAA AAACGGAGCG
    AGCTGCGATG CCGTGATGGG ACAGTGCATT TGTCCCTCGG GGTACCATGG TATCCACTGT
    GAGAAAGGCT GTGCCAGTGG GCGCTTCGGT GAGCGGTGCC AGCGCCAGTG CGACTGTGGA
    GGAGCTCCCT GCGACCCGGC CACTGGGAAA TGCCTCTGCC CACCTGGAAA GACTGGGGAC
    AAGTGTGACA TTGGCTGCCG GTCTGACCAG TACGGCCCCG ACTGCTCTCT GCGATGCCAG
    TGTGCCAGCA AGTCCCAGTG CAACCCATAC AATGGGAAAT GTGTCTGTCC AGCCACGTGG
    ATGGGGCCAA CCTGCAAGGA AGGTGGCCCT CCAAAGCTTC CTTTTTATGA AGAACAAACT
    CAAGAAAGAG GGAATATTTT TCCGAGAGCT CTACAACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40120
    Clone ID Related Accession (Same CDS sequence) XM_015291738.1 , XM_015291739.1 , XM_015291740.1 , XM_015291734.1 , XM_015291735.1 , XM_015291741.1 , XM_015291742.1 , XM_015291737.2 , XM_015291733.1 , XM_015291737.1
    Accession Version XM_015291737.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGA ATGTCTCCCT
    GGCAAGTACG GCCCCAACTG CAGCTTGGAC TGCTCATGCC AGCACAACGG CACCTGCGAC
    AGGTTCACGG GCTGCTGCCT CTGCCCCGCG GGGTTCTACG GGCGCTCCTG TGAGCACGGG
    TGCCCCCCCG GGTTTTATGG GCTGAGCTGC CTTCATGCAT GTGCCTGTAA AAACGGAGCG
    AGCTGCGATG CCGTGATGGG ACAGTGCATT TGTCCCTCGG GGTACCATGG TATCCACTGT
    GAGAAAGGCT GTGCCAGTGG GCGCTTCGGT GAGCGGTGCC AGCGCCAGTG CGACTGTGGA
    GGAGCTCCCT GCGACCCGGC CACTGGGAAA TGCCTCTGCC CACCTGGAAA GACTGGGGAC
    AAGTGTGACA TTGGCTGCCG GTCTGACCAG TACGGCCCCG ACTGCTCTCT GCGATGCCAG
    TGTGCCAGCA AGTCCCAGTG CAACCCATAC AATGGGAAAT GTGTCTGTCC AGCCACGTGG
    ATGGGGCCAA CCTGCAAGGA AGGTGGCCCT CCAAAGCTTC CTTTTTATGA AGAACAAACT
    CAAGAAAGAG GGAATATTTT TCCGAGAGCT CTACAACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147223.1
    CDS176..4717
    Translation

    Target ORF information:

    RefSeq Version XM_015291737.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291737.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCCAGGGTGT CCAGAAGGTA CTTACGGTTC AAACTGCCAA
    AAATCCTGTA AATGCATGAA TGGAGGCCAT TGCGACCCAG TGTCGGGAAC TTGTGATTGC
    GCGCCTGGTT TCATTGGAGC CGACTGTAGT AAAACTTGCC CAGAAAATCG ATACGGGAAG
    GACTGCGCCC TGTTCTGTGC ATGCGGTAAA GGTCATTGTG ACCCACGGAC TGGCAGCTGT
    ACCTGTCCTC CTGGCCAAAT GGGACCTGGC TGTCAGCAAG TGTGTCCCCA GGGACGGTTC
    GGCCCCAGCT GCCGCCTGAC ATGCAGCTGT CAGAACGGTG CTATCTGTGA CCACGTGGAT
    GGCAGCTGCA CTTGTGGACT TGGCTGGACA GGAAAATCGT GTGAGAAAGA ATGTCTCCCT
    GGCAAGTACG GCCCCAACTG CAGCTTGGAC TGCTCATGCC AGCACAACGG CACCTGCGAC
    AGGTTCACGG GCTGCTGCCT CTGCCCCGCG GGGTTCTACG GGCGCTCCTG TGAGCACGGG
    TGCCCCCCCG GGTTTTATGG GCTGAGCTGC CTTCATGCAT GTGCCTGTAA AAACGGAGCG
    AGCTGCGATG CCGTGATGGG ACAGTGCATT TGTCCCTCGG GGTACCATGG TATCCACTGT
    GAGAAAGGCT GTGCCAGTGG GCGCTTCGGT GAGCGGTGCC AGCGCCAGTG CGACTGTGGA
    GGAGCTCCCT GCGACCCGGC CACTGGGAAA TGCCTCTGCC CACCTGGAAA GACTGGGGAC
    AAGTGTGACA TTGGCTGCCG GTCTGACCAG TACGGCCCCG ACTGCTCTCT GCGATGCCAG
    TGTGCCAGCA AGTCCCAGTG CAACCCATAC AATGGGAAAT GTGTCTGTCC AGCCACGTGG
    ATGGGGCCAA CCTGCAAGGA AGGTGGCCCT CCAAAGCTTC CTTTTTATGA AGAACAAACT
    CAAGAAAGAG GGAATATTTT TCCGAGAGCT CTACAACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40121
    Clone ID Related Accession (Same CDS sequence) XM_015291743.1
    Accession Version XM_015291743.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4539bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCGGTGTCCA GAAGGTACTT ACGGTTCAAA CTGCCAAAAA
    TCCTGTAAAT GCATGAATGG AGGCCATTGC GACCCAGTGT CGGGAACTTG TGATTGCGCG
    CCTGGTTTCA TTGGAGCCGA CTGTAGTAAA ACTTGCCCAG AAAATCGATA CGGGAAGGAC
    TGCGCCCTGT TCTGTGCATG CGGTAAAGGT CATTGTGACC CACGGACTGG CAGCTGTACC
    TGTCCTCCTG GCCAAATGGG ACCTGGCTGT CAGCAAGTGT GTCCCCAGGG ACGGTTCGGC
    CCCAGCTGCC GCCTGACATG CAGCTGTCAG AACGGTGCTA TCTGTGACCA CGTGGATGGC
    AGCTGCACTT GTGGACTTGG CTGGACAGGA AAATCGTGTG AGAAAGAATG TCTCCCTGGC
    AAGTACGGCC CCAACTGCAG CTTGGACTGC TCATGCCAGC ACAACGGCAC CTGCGACAGG
    TTCACGGGCT GCTGCCTCTG CCCCGCGGGG TTCTACGGGC GCTCCTGTGA GCACGGGTGC
    CCCCCCGGGT TTTATGGGCT GAGCTGCCTT CATGCATGTG CCTGTAAAAA CGGAGCGAGC
    TGCGATGCCG TGATGGGACA GTGCATTTGT CCCTCGGGGT ACCATGGTAT CCACTGTGAG
    AAAGGCTGTG CCAGTGGGCG CTTCGGTGAG CGGTGCCAGC GCCAGTGCGA CTGTGGAGGA
    GCTCCCTGCG ACCCGGCCAC TGGGAAATGC CTCTGCCCAC CTGGAAAGAC TGGGGACAAG
    TGTGACATTG GCTGCCGGTC TGACCAGTAC GGCCCCGACT GCTCTCTGCG ATGCCAGTGT
    GCCAGCAAGT CCCAGTGCAA CCCATACAAT GGGAAATGTG TCTGTCCAGC CACGTGGATG
    GGGCCAACCT GCAAGGAAGG TGGCCCTCCA AAGCTTCCTT TTTATGAAGA ACAAACTCAA
    GAAAGAGGGA ATATTTTTCC GAGAGCTCTA CAACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147229.1
    CDS175..4713
    Translation

    Target ORF information:

    RefSeq Version XM_015291743.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291743.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGTGTTA GGAGGGGACT CAGCAGTGTC GGGCTTCGCA
    GGGGCTCCGG GGCTGCAGTT CAGAGGTGCC CCCCAGCTCC TGCACTATGT GCTTGGGGCC
    CCACGTGAGG ATGAGGAGCA GCGAGGAGAG CTGACACTGA TACACAGAGC CGTTTGCCTT
    GATAACACTT TTGGCAATGA CTGCAGCTTG AGCTGTGAGG ATTGTATGAA TGGAGGCAGA
    TGTAACAGTG AGAACAGTGG CTGTGTTTGC TCACCTGGGT GGACCGGCAT CATCTGCAAC
    CAAACCTGCG CCCAGGGTAC ATACGGTGAT GGTTGTGCCA GTATCTGCAT GTGCCAGAAT
    GGAGGCTCCT GTGACCCCAT CACAGGGCTT TGTCACTGCC CACCAGGAGT CCAGGGGCCA
    TTTTGTGAAG ACGGTTGTCC CAGGGGATTT TTTGGGAAGA ACTGCAACAA ACCATGCAAC
    TGTGCCAACA GCGGCCACTG CCACCGGCTA TATGGAGCCT GCCTGTGTGA CCCTGGGCTG
    TATGGACGCT TCTGCCACCT CACCTGTCCC AGGTGGGCTT TTGGTGTGGG CTGCTCACAG
    GAGTGTCAGT GTGTGAAGGA GCACACACTG GGATGCAGCG CTCGGAACGG GACCTGCAGC
    TGCAAGCCTG GATATCACGG CAAGAAGTGC CAGAAAGCAT GCACACCTGG CTTTTATGGG
    GCTGGTTGCA AACTGAGGTG CAACTGTTCC ACCGACGTTT TGTGTGACCA CGAGACGGGA
    GCCTGCAAGC GTCCGTGCCC GCCTGGTTTC CATGGAGAAA AGTGCCATTC CTCTTGCCAG
    CCCGGGAGCT TTGGGGTGGA CTGCAGGCAG AGCTGCAGCT GCGGGGGAGC ACCGTGTGAC
    CCAGAGACTG GGCGTTGTTT CTGCCCAGCT GGGAAAACTG GTGCCATGTG TGAGAGAGCC
    TGCCCTGTTG GATACTACGG ACAAAACTGC CAGCTGCCAT GCAGCTGCAG CAGTGATGCT
    CTGTGTCACC ACATCACTGG GGAGTGCACG TGTCCCCCCG GCTGGACTGG CCACGACTGC
    AAGCACCCCT GCAATAGCGG CCGTTGGGGA CAGCACTGTG AAAACTCCTG TGCCTGCAAT
    AACAGCGATG GGAGCTGCGA TCCTGTCACT GGGTCCTGCT TCTGTGAGCC AGGATTCACT
    GGGAAACACT GCGAGCAGAG ATGTCCTGAG GGAAGCTTTG GTCCCAGCTG CATGCACAGC
    TGCCAGTGCC AGAACGGGGC TGCATGTGAC CATGTGAGCG GAGCCTGCAC GTGCAGTGCT
    GGATGGACCG GGACCTTCTG CAGGAAAGCT TGTCCAGAAG GATTTTTTGG GCTTGACTGC
    CACCAGGTGT GCAACTGCAA GAACGCTGAG GGCTGTGACC ATGTCCTGGG GACGTGCCGC
    TGCCTCCCAG GCTGGAGGGG AGAGACCTGC GAGCAGCCGT GTCAGGGGGA CAGGTTTGGG
    CAGGGCTGTG CTCACACCTG CAACTGTCAC AACGGAGCAC TCTGCGACCA CATAACGGGG
    ACATGTGCCT GCACCCCAGG CTGGGGAGGA GCCACCTGCC AAGAAGCCTG TCCCCCAGGC
    TGGCATGGTG CTGGCTGCCT TCAGCGCTGC ATTTGCCCTG GCCAGACTGC ATGTGACCCC
    GTGACGGGCA AGTGCCAGTG TTCCAAGGGC GGAACAGGGA CATCCTGTGA GCTTGCATGT
    CCCCCAGGAT TTTTTGGAGA GAGATGTGAA GAGCAATGTG ACTGCATTCA CAGCGTGTCC
    TGCCACCCCC AAACGGGGGC TTGTCGCTGT GCCAAGGGGT GGCGAGGCAG GCACTGTGAC
    AAACCCTGCT TGCCTGGCCA CTACGGCATG GGTTGTGCCC GGCGGTGCCA CTGCCCTGCA
    GGCACCCCCT GCCACCACCT GACGGGTGAG TGCAGCTGTC CTCCAGGATT CACTGGCCAT
    GGCTGCGAGA AAACTTGTCC ACCAAACACG TATGGCCAGA GTTGCAGCCA AGCCTGTCGC
    TGCCGTGCTG AGGAGGACTG TCACCCCATC ACTGGGCGGT GCGTGTGCCG CCTGGGGTAC
    CACGGGGCCC AGTGTGACCG CCGGTGTCCA GAAGGTACTT ACGGTTCAAA CTGCCAAAAA
    TCCTGTAAAT GCATGAATGG AGGCCATTGC GACCCAGTGT CGGGAACTTG TGATTGCGCG
    CCTGGTTTCA TTGGAGCCGA CTGTAGTAAA ACTTGCCCAG AAAATCGATA CGGGAAGGAC
    TGCGCCCTGT TCTGTGCATG CGGTAAAGGT CATTGTGACC CACGGACTGG CAGCTGTACC
    TGTCCTCCTG GCCAAATGGG ACCTGGCTGT CAGCAAGTGT GTCCCCAGGG ACGGTTCGGC
    CCCAGCTGCC GCCTGACATG CAGCTGTCAG AACGGTGCTA TCTGTGACCA CGTGGATGGC
    AGCTGCACTT GTGGACTTGG CTGGACAGGA AAATCGTGTG AGAAAGAATG TCTCCCTGGC
    AAGTACGGCC CCAACTGCAG CTTGGACTGC TCATGCCAGC ACAACGGCAC CTGCGACAGG
    TTCACGGGCT GCTGCCTCTG CCCCGCGGGG TTCTACGGGC GCTCCTGTGA GCACGGGTGC
    CCCCCCGGGT TTTATGGGCT GAGCTGCCTT CATGCATGTG CCTGTAAAAA CGGAGCGAGC
    TGCGATGCCG TGATGGGACA GTGCATTTGT CCCTCGGGGT ACCATGGTAT CCACTGTGAG
    AAAGGCTGTG CCAGTGGGCG CTTCGGTGAG CGGTGCCAGC GCCAGTGCGA CTGTGGAGGA
    GCTCCCTGCG ACCCGGCCAC TGGGAAATGC CTCTGCCCAC CTGGAAAGAC TGGGGACAAG
    TGTGACATTG GCTGCCGGTC TGACCAGTAC GGCCCCGACT GCTCTCTGCG ATGCCAGTGT
    GCCAGCAAGT CCCAGTGCAA CCCATACAAT GGGAAATGTG TCTGTCCAGC CACGTGGATG
    GGGCCAACCT GCAAGGAAGG TGGCCCTCCA AAGCTTCCTT TTTATGAAGA ACAAACTCAA
    GAAAGAGGGA ATATTTTTCC GAGAGCTCTA CAACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40122
    Clone ID Related Accession (Same CDS sequence) XM_015291744.1
    Accession Version XM_015291744.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4506bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product multiple epidermal growth factor-like domains protein 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455917.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGGGGCT CCGGGGCTGC AGTTCAGAGG TGCCCCCCAG
    CTCCTGCACT ATGTGCTTGG GGCCCCACGT GAGGATGAGG AGCAGCGAGG AGAGCTGACA
    CTGATACACA GAGCCGTTTG CCTTGATAAC ACTTTTGGCA ATGACTGCAG CTTGAGCTGT
    GAGGATTGTA TGAATGGAGG CAGATGTAAC AGTGAGAACA GTGGCTGTGT TTGCTCACCT
    GGGTGGACCG GCATCATCTG CAACCAAACC TGCGCCCAGG GTACATACGG TGATGGTTGT
    GCCAGTATCT GCATGTGCCA GAATGGAGGC TCCTGTGACC CCATCACAGG GCTTTGTCAC
    TGCCCACCAG GAGTCCAGGG GCCATTTTGT GAAGACGGTT GTCCCAGGGG ATTTTTTGGG
    AAGAACTGCA ACAAACCATG CAACTGTGCC AACAGCGGCC ACTGCCACCG GCTATATGGA
    GCCTGCCTGT GTGACCCTGG GCTGTATGGA CGCTTCTGCC ACCTCACCTG TCCCAGGTGG
    GCTTTTGGTG TGGGCTGCTC ACAGGAGTGT CAGTGTGTGA AGGAGCACAC ACTGGGATGC
    AGCGCTCGGA ACGGGACCTG CAGCTGCAAG CCTGGATATC ACGGCAAGAA GTGCCAGAAA
    GCATGCACAC CTGGCTTTTA TGGGGCTGGT TGCAAACTGA GGTGCAACTG TTCCACCGAC
    GTTTTGTGTG ACCACGAGAC GGGAGCCTGC AAGCGTCCGT GCCCGCCTGG TTTCCATGGA
    GAAAAGTGCC ATTCCTCTTG CCAGCCCGGG AGCTTTGGGG TGGACTGCAG GCAGAGCTGC
    AGCTGCGGGG GAGCACCGTG TGACCCAGAG ACTGGGCGTT GTTTCTGCCC AGCTGGGAAA
    ACTGGTGCCA TGTGTGAGAG AGCCTGCCCT GTTGGATACT ACGGACAAAA CTGCCAGCTG
    CCATGCAGCT GCAGCAGTGA TGCTCTGTGT CACCACATCA CTGGGGAGTG CACGTGTCCC
    CCCGGCTGGA CTGGCCACGA CTGCAAGCAC CCCTGCAATA GCGGCCGTTG GGGACAGCAC
    TGTGAAAACT CCTGTGCCTG CAATAACAGC GATGGGAGCT GCGATCCTGT CACTGGGTCC
    TGCTTCTGTG AGCCAGGATT CACTGGGAAA CACTGCGAGC AGAGATGTCC TGAGGGAAGC
    TTTGGTCCCA GCTGCATGCA CAGCTGCCAG TGCCAGAACG GGGCTGCATG TGACCATGTG
    AGCGGAGCCT GCACGTGCAG TGCTGGATGG ACCGGGACCT TCTGCAGGAA AGCTTGTCCA
    GAAGGATTTT TTGGGCTTGA CTGCCACCAG GTGTGCAACT GCAAGAACGC TGAGGGCTGT
    GACCATGTCC TGGGGACGTG CCGCTGCCTC CCAGGCTGGA GGGGAGAGAC CTGCGAGCAG
    CCGTGTCAGG GGGACAGGTT TGGGCAGGGC TGTGCTCACA CCTGCAACTG TCACAACGGA
    GCACTCTGCG ACCACATAAC GGGGACATGT GCCTGCACCC CAGGCTGGGG AGGAGCCACC
    TGCCAAGAAG CCTGTCCCCC AGGCTGGCAT GGTGCTGGCT GCCTTCAGCG CTGCATTTGC
    CCTGGCCAGA CTGCATGTGA CCCCGTGACG GGCAAGTGCC AGTGTTCCAA GGGCGGAACA
    GGGACATCCT GTGAGCTTGC ATGTCCCCCA GGATTTTTTG GAGAGAGATG TGAAGAGCAA
    TGTGACTGCA TTCACAGCGT GTCCTGCCAC CCCCAAACGG GGGCTTGTCG CTGTGCCAAG
    GGGTGGCGAG GCAGGCACTG TGACAAACCC TGCTTGCCTG GCCACTACGG CATGGGTTGT
    GCCCGGCGGT GCCACTGCCC TGCAGGCACC CCCTGCCACC ACCTGACGGG TGAGTGCAGC
    TGTCCTCCAG GATTCACTGG CCATGGCTGC GAGAAAACTT GTCCACCAAA CACGTATGGC
    CAGAGTTGCA GCCAAGCCTG TCGCTGCCGT GCTGAGGAGG ACTGTCACCC CATCACTGGG
    CGGTGCGTGT GCCGCCTGGG GTACCACGGG GCCCAGTGTG ACCGCCCAGG GTGTCCAGAA
    GGTACTTACG GTTCAAACTG CCAAAAATCC TGTAAATGCA TGAATGGAGG CCATTGCGAC
    CCAGTGTCGG GAACTTGTGA TTGCGCGCCT GGTTTCATTG GAGCCGACTG TAGTAAAACT
    TGCCCAGAAA ATCGATACGG GAAGGACTGC GCCCTGTTCT GTGCATGCGG TAAAGGTCAT
    TGTGACCCAC GGACTGGCAG CTGTACCTGT CCTCCTGGCC AAATGGGACC TGGCTGTCAG
    CAAGTGTGTC CCCAGGGACG GTTCGGCCCC AGCTGCCGCC TGACATGCAG CTGTCAGAAC
    GGTGCTATCT GTGACCACGT GGATGGCAGC TGCACTTGTG GACTTGGCTG GACAGGAAAA
    TCGTGTGAGA AAGAATGTCT CCCTGGCAAG TACGGCCCCA ACTGCAGCTT GGACTGCTCA
    TGCCAGCACA ACGGCACCTG CGACAGGTTC ACGGGCTGCT GCCTCTGCCC CGCGGGGTTC
    TACGGGCGCT CCTGTGAGCA CGGGTGCCCC CCCGGGTTTT ATGGGCTGAG CTGCCTTCAT
    GCATGTGCCT GTAAAAACGG AGCGAGCTGC GATGCCGTGA TGGGACAGTG CATTTGTCCC
    TCGGGGTACC ATGGTATCCA CTGTGAGAAA GGCTGTGCCA GTGGGCGCTT CGGTGAGCGG
    TGCCAGCGCC AGTGCGACTG TGGAGGAGCT CCCTGCGACC CGGCCACTGG GAAATGCCTC
    TGCCCACCTG GAAAGACTGG GGACAAGTGT GACATTGGCT GCCGGTCTGA CCAGTACGGC
    CCCGACTGCT CTCTGCGATG CCAGTGTGCC AGCAAGTCCC AGTGCAACCC ATACAATGGG
    AAATGTGTCT GTCCAGCCAC GTGGATGGGG CCAACCTGCA AGGAAGGTGG CCCTCCAAAG
    CTTCCTTTTT ATGAAGAACA AACTCAAGAA AGAGGGAATA TTTTTCCGAG AGCTCTACAA
    CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147230.1
    CDS176..4681
    Translation

    Target ORF information:

    RefSeq Version XM_015291744.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus multiple EGF-like-domains 6-like (LOC424998), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291744.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATTGGAA AACGATGTGC TCTGCGGGAG GTAATGAGAT TAGCCCGACC CAGAGTCCTG 
    CGGAGCTCCA ACTGCTCCAG ATGCATGGAA AGGAGGGGAG GGGAGGGCTC AGCCGTGCGG
    TGCGGATCTC AGGGTACAAT TCTAAGGTCA GGGAAGGGCT CTATGTTTCC TGGCCGTGGA
    GAAGAAATCC CCCCGCTGCT CTCAGCCGCA GAAGTTAATT GGGGGCAACT TAAAGCGAGG
    CCAAATGTCT GCCCTGTGTT GGAGCTGGTG CTGGTGGGAC ACCAACAACC ATGTGTTCAG
    GCCTTCAGCC GCATGGTGAA GATGTGGAAA CAAGGCTGCA CAGGGAGGAG ATGGTGCATG
    GGATATGAGA GGAGGTCTGG TTACTACACT GTGTATAAGC AGGTGTACAG AATGGAGCAC
    CAGACTGTCT ATAAGTGCTG CCCTGGGTGG GCTCAACAGA ACAGCGAGCT TGGCTGCTTG
    CACATGCTCT GTGCTGCTGA CACTTGCTTC AATGGAGGGA AGTGTTCTGA AGAAGGACCT
    CACATGTGCC AGTGTCCTGC GGGATTTCAG GGTCCTCGCT GCCAGTATGA TGTAAATGAG
    TGCAGCACTG AGAACGGAGG CTGTCAGGAC CAGTGCTGTA ACACCATCGG CAGCTACCAC
    TGCAAATGTC CAGCTGGCCA GAAACTGGGG GAAGATGGAA AATCGTGCGG AGATATAGAT
    GAGTGTGCAG AGGTGAATGG AGGCTGCCAG CAGGGCTGCA TCAACACCCA CAGCTCTTTC
    CACTGCCACT GTTGGGCAGG ATACAGGCTG CACTCTGATG GGCGCACGTG CTTGATGATA
    GACCCCTGCA CAAGTGGGAA TGGAGGATGC TCACAAATCT GTCAAAATGA GAGAGGAACT
    GCCAAATGTG ACTGTCACCC AGGGCATTAT CTTTCTACAG ATGAGAAGTC CTGCTTAGAC
    ATTGATGAGT GTGCAGAAGG GAAAGCCAGG TGTGCTCATC ACTGTGTGAA CATGCTGGGA
    TCCTTCACCT GTGCCTGTAA TCCCGGCTTC GAGCTGGGGG CTGATGGGAA GCAGTGCTAC
    CGCATTGAAA TGGAGATAGT GGACAGCTGC CAGCAGGACA ACGGTGGGTG CTCCCACCGC
    TGCGAGCACA CAGCAGCAGG ACCACGCTGC TCCTGCCACG ATGGCTACGT GCTCAGCTCA
    GATGGCAAGG CATGCACAGA CCTGGATGAG TGCGAGACCG GCGAGTCGTG CTGCTCCCAG
    TTCTGCATCA ACTACGTGGG CAGCTACGAG TGCACCTGTA GAGCAGGCTT CCAGCCCAGT
    GCCGATGGCT GCAGCTGCGA CGATGTGGAT GAATGTCGTC TTGAGAATGG CAACTGTGAC
    CATATCTGCA TTAATTCCCT GGGGTCATAC GAATGCGCCT GCAAGGAGAG GTACCGGCTG
    GGTGCTGACC GGCGCTCCTG CATTGGGGCT CCGGGGCTGC AGTTCAGAGG TGCCCCCCAG
    CTCCTGCACT ATGTGCTTGG GGCCCCACGT GAGGATGAGG AGCAGCGAGG AGAGCTGACA
    CTGATACACA GAGCCGTTTG CCTTGATAAC ACTTTTGGCA ATGACTGCAG CTTGAGCTGT
    GAGGATTGTA TGAATGGAGG CAGATGTAAC AGTGAGAACA GTGGCTGTGT TTGCTCACCT
    GGGTGGACCG GCATCATCTG CAACCAAACC TGCGCCCAGG GTACATACGG TGATGGTTGT
    GCCAGTATCT GCATGTGCCA GAATGGAGGC TCCTGTGACC CCATCACAGG GCTTTGTCAC
    TGCCCACCAG GAGTCCAGGG GCCATTTTGT GAAGACGGTT GTCCCAGGGG ATTTTTTGGG
    AAGAACTGCA ACAAACCATG CAACTGTGCC AACAGCGGCC ACTGCCACCG GCTATATGGA
    GCCTGCCTGT GTGACCCTGG GCTGTATGGA CGCTTCTGCC ACCTCACCTG TCCCAGGTGG
    GCTTTTGGTG TGGGCTGCTC ACAGGAGTGT CAGTGTGTGA AGGAGCACAC ACTGGGATGC
    AGCGCTCGGA ACGGGACCTG CAGCTGCAAG CCTGGATATC ACGGCAAGAA GTGCCAGAAA
    GCATGCACAC CTGGCTTTTA TGGGGCTGGT TGCAAACTGA GGTGCAACTG TTCCACCGAC
    GTTTTGTGTG ACCACGAGAC GGGAGCCTGC AAGCGTCCGT GCCCGCCTGG TTTCCATGGA
    GAAAAGTGCC ATTCCTCTTG CCAGCCCGGG AGCTTTGGGG TGGACTGCAG GCAGAGCTGC
    AGCTGCGGGG GAGCACCGTG TGACCCAGAG ACTGGGCGTT GTTTCTGCCC AGCTGGGAAA
    ACTGGTGCCA TGTGTGAGAG AGCCTGCCCT GTTGGATACT ACGGACAAAA CTGCCAGCTG
    CCATGCAGCT GCAGCAGTGA TGCTCTGTGT CACCACATCA CTGGGGAGTG CACGTGTCCC
    CCCGGCTGGA CTGGCCACGA CTGCAAGCAC CCCTGCAATA GCGGCCGTTG GGGACAGCAC
    TGTGAAAACT CCTGTGCCTG CAATAACAGC GATGGGAGCT GCGATCCTGT CACTGGGTCC
    TGCTTCTGTG AGCCAGGATT CACTGGGAAA CACTGCGAGC AGAGATGTCC TGAGGGAAGC
    TTTGGTCCCA GCTGCATGCA CAGCTGCCAG TGCCAGAACG GGGCTGCATG TGACCATGTG
    AGCGGAGCCT GCACGTGCAG TGCTGGATGG ACCGGGACCT TCTGCAGGAA AGCTTGTCCA
    GAAGGATTTT TTGGGCTTGA CTGCCACCAG GTGTGCAACT GCAAGAACGC TGAGGGCTGT
    GACCATGTCC TGGGGACGTG CCGCTGCCTC CCAGGCTGGA GGGGAGAGAC CTGCGAGCAG
    CCGTGTCAGG GGGACAGGTT TGGGCAGGGC TGTGCTCACA CCTGCAACTG TCACAACGGA
    GCACTCTGCG ACCACATAAC GGGGACATGT GCCTGCACCC CAGGCTGGGG AGGAGCCACC
    TGCCAAGAAG CCTGTCCCCC AGGCTGGCAT GGTGCTGGCT GCCTTCAGCG CTGCATTTGC
    CCTGGCCAGA CTGCATGTGA CCCCGTGACG GGCAAGTGCC AGTGTTCCAA GGGCGGAACA
    GGGACATCCT GTGAGCTTGC ATGTCCCCCA GGATTTTTTG GAGAGAGATG TGAAGAGCAA
    TGTGACTGCA TTCACAGCGT GTCCTGCCAC CCCCAAACGG GGGCTTGTCG CTGTGCCAAG
    GGGTGGCGAG GCAGGCACTG TGACAAACCC TGCTTGCCTG GCCACTACGG CATGGGTTGT
    GCCCGGCGGT GCCACTGCCC TGCAGGCACC CCCTGCCACC ACCTGACGGG TGAGTGCAGC
    TGTCCTCCAG GATTCACTGG CCATGGCTGC GAGAAAACTT GTCCACCAAA CACGTATGGC
    CAGAGTTGCA GCCAAGCCTG TCGCTGCCGT GCTGAGGAGG ACTGTCACCC CATCACTGGG
    CGGTGCGTGT GCCGCCTGGG GTACCACGGG GCCCAGTGTG ACCGCCCAGG GTGTCCAGAA
    GGTACTTACG GTTCAAACTG CCAAAAATCC TGTAAATGCA TGAATGGAGG CCATTGCGAC
    CCAGTGTCGG GAACTTGTGA TTGCGCGCCT GGTTTCATTG GAGCCGACTG TAGTAAAACT
    TGCCCAGAAA ATCGATACGG GAAGGACTGC GCCCTGTTCT GTGCATGCGG TAAAGGTCAT
    TGTGACCCAC GGACTGGCAG CTGTACCTGT CCTCCTGGCC AAATGGGACC TGGCTGTCAG
    CAAGTGTGTC CCCAGGGACG GTTCGGCCCC AGCTGCCGCC TGACATGCAG CTGTCAGAAC
    GGTGCTATCT GTGACCACGT GGATGGCAGC TGCACTTGTG GACTTGGCTG GACAGGAAAA
    TCGTGTGAGA AAGAATGTCT CCCTGGCAAG TACGGCCCCA ACTGCAGCTT GGACTGCTCA
    TGCCAGCACA ACGGCACCTG CGACAGGTTC ACGGGCTGCT GCCTCTGCCC CGCGGGGTTC
    TACGGGCGCT CCTGTGAGCA CGGGTGCCCC CCCGGGTTTT ATGGGCTGAG CTGCCTTCAT
    GCATGTGCCT GTAAAAACGG AGCGAGCTGC GATGCCGTGA TGGGACAGTG CATTTGTCCC
    TCGGGGTACC ATGGTATCCA CTGTGAGAAA GGCTGTGCCA GTGGGCGCTT CGGTGAGCGG
    TGCCAGCGCC AGTGCGACTG TGGAGGAGCT CCCTGCGACC CGGCCACTGG GAAATGCCTC
    TGCCCACCTG GAAAGACTGG GGACAAGTGT GACATTGGCT GCCGGTCTGA CCAGTACGGC
    CCCGACTGCT CTCTGCGATG CCAGTGTGCC AGCAAGTCCC AGTGCAACCC ATACAATGGG
    AAATGTGTCT GTCCAGCCAC GTGGATGGGG CCAACCTGCA AGGAAGGTGG CCCTCCAAAG
    CTTCCTTTTT ATGAAGAACA AACTCAAGAA AGAGGGAATA TTTTTCCGAG AGCTCTACAA
    CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.