LOC424588 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC424588
Entrez Gene ID	424588
Full Name	E3 ubiquitin-protein ligase HECTD3-like
General protein information	Preferred Names E3 ubiquitin-protein ligase HECTD3-like Names E3 ubiquitin-protein ligase HECTD3-like
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_422428.5	XP_422428.3	E3 ubiquitin-protein ligase HECTD3-like isoform X2
XM_025153082.1	XP_025008850.1	E3 ubiquitin-protein ligase HECTD3 isoform X3
XM_015291285.2	XP_015146771.1	E3 ubiquitin-protein ligase HECTD3 isoform X4
XM_015291283.1	XP_015146769.1	E3 ubiquitin-protein ligase HECTD3-like isoform X1
XM_015291283.2	XP_015146769.1	E3 ubiquitin-protein ligase HECTD3 isoform X1
XM_422428.6	XP_422428.3	E3 ubiquitin-protein ligase HECTD3 isoform X2
XM_015291285.1	XP_015146771.1	E3 ubiquitin-protein ligase HECTD3-like isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC424588 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC424588 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa152481	XM_025153082.1 Latest version!	Gallus gallus E3 ubiquitin-protein ligase HECTD3-like (HECTD3L), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OGa39557	XM_015291283.2 Latest version!	Gallus gallus E3 ubiquitin-protein ligase HECTD3-like (HECTD3L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OGa39558	XM_422428.6 Latest version!	Gallus gallus E3 ubiquitin-protein ligase HECTD3-like (HECTD3L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OGa39559	XM_015291285.2 Latest version!	Gallus gallus E3 ubiquitin-protein ligase HECTD3-like (HECTD3L), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
View Old Accession Versions >>
OGa39557	XM_015291283.1	Gallus gallus E3 ubiquitin-protein ligase HECTD3-like (LOC424588), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OGa39558	XM_422428.5	Gallus gallus E3 ubiquitin-protein ligase HECTD3-like (LOC424588), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OGa39559	XM_015291285.1	Gallus gallus E3 ubiquitin-protein ligase HECTD3-like (LOC424588), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC424588 ( XM_025153082.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025008850.1 Gallus gallus E3 ubiquitin-protein ligase HECTD3-like (HECTD3L), transcript variant X3, mRNA.

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CloneID	OGa152481
Clone ID Related Accession (Same CDS sequence)	XM_025153082.1
Accession Version	XM_025153082.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase HECTD3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTTT TTCCGGCGCA CGGCCTGCTC GAGCACCTGG AGGAGCACGG GGTCTGCCGT 
GAGCTGGAGG AGGGCTGGGT CCTGGCGCAG CGGTTCGGAG ATGGGGGAGA TAAGTTGGTG 
CCGGTGGAGA GGATCCAGCA GCAACAGCAG ACCTTTGGAA CGGATTACAA ACCTGCGGCC 
AGCTGGGAAC AAGTGGTGGA CCTGATGTAC TCCATGCAGC TGGGAGAGAA GCCCGTGCCA 
GTCGAGCCGG ATGAGGCTGT GGTGCAGAAG CTTCGGTCGA TGCCCCCCAA TTGGAGCTAC 
GAGCAGGACA TGGAGCTGGG GCGCTTCCTG TACGACGTCA CTGAGAGGGA TGAACACTGC 
AAGGACTGCG TTAAGGACCA CCTCAATGGC ATAGAGGTCT CCTCACAACT GGATGACTTC 
AAAGTATCCC ACCTGACAGA CAACCAGGGA GACACCTACT GGGAGAGCAG CGGGTCCCCT 
GGGCAGCATT GGGTGCGGCT CCACATGAAG AAAGGCAGCG TCATCAAGAA GCTGTGGCTG 
ATGCTGAACG CAAACATCAA CTCCTACGTC CCCAGGAGGG TGGCTGTGTA CGGAGGGCCA 
GCCAACAGGC TGCAGCACCT GAGAACCGTC CTAATCAACG ACCGCTCCTC TCTGCTCCAT 
CCCCTCCTGC TGCAGCCCTG GAACAGCTAT CAAGATGTGT GCATCCTACG TGACATGAAG 
ACCCACGTGC CGGTCCTGGA GATCCGCATC TTGGAGTGCC GAGAACAAGG GTACAACGTC 
CGGCTGCGAG GGATTAAGTT CAAGTCCTTC TGGGAGAGGG ACCTGAACCT CAACGCAGAC 
ATGTTCCAGA TGGCCCAGCT GGTGCGCTAC CCACTGCTGG AAGGGGTGGA CACAGATGTG 
CTGTACCGCA GGGCTGTAGT CATCCAGAGG TTCACCCAGC TCCTGGACAG TGTCCTGGGG 
TACCTGATCC CCATCTCTGA TGAAAGTGAC AGCACCTTCA GTGTGCTCAG GGGCATGAAG 
CCATTCCTGC AGCTGTGCAA GCTGAGCAAA ACCCTCGTCA CCCATTGCCT GCAGAGCTCA 
GAGAGCAGCC CACCTTGCAT GCTGCCAAAG CTGCTCATCA ACCGGCAGCT GGCCCGTGAG 
CACCGGGCTC ACCCTGAGCT GGACCCTAGT GGCCGGAACA CAGTCTTCAC CCAGGTGTAT 
GAAAACCTGA AGCCTTCCAA AACCAACAAC CATCTCCTAA ACTACAGGTG GCCTCAGACC 
CACAGCCAGT GGTGGGAGTG TGTCTTCACC ACGGAGGGCA TCATCGATAA TGGCGGTGGT 
TTTCGGGACT GCTTGACAGA CATCTCAGAA GAGCTGTGTC CCAGCTCGGG CGATGTCCCC 
GTGCCTCTGC CCTTCTTTGT GCGCACATCC AACCAGGGTA ACAGCAGCAG CGACACCAGG 
GACATGTACG TCCCCAACCC CTCCTGCAAG GACTTCCCTA AGTATGAGTG GATCGGACAG 
CTCATGGGAG CAGCCCTGAG GAGCAAGGAG ATTCTGGTCC TGGCTCTGCC CAGTCTGGTG 
TGGAAGCAGC TGTCAGGGGA GGAGGTCATC TGGAGCAAGG ACTTTGCTGC TGTGGATGTG 
GAGCTGGTGA AGCTGCTGCA GATGCTGGAG GAAGTGGACA GGGAGGCCTT CAACTTCATG 
TTTGGCAAGG AGCTGACCTA CACAACGGTA CGGAGCGACC AGCGCGTGGT GGAGCTGATC 
CCAAAGGGCA GCAGCACTGT GGTGCGCTTT GAGGACCGCA AGGAGTTCAT CCGTCTGGTG 
CAGAAGGCAC GGCTGGAGGA GAGCAAGGAG CAGGTGGAGG CCATTCGTGC CGGGCTGCTC 
CGTGTGGTGC CTCAGGCTGT GCTGGACCTG CTCACCTGGC AGCAGCTGGA GAAGAAGGTC 
TGTGGGAACC CCGAGGTGAC GGTGGACGAG CTGAAGAAGT TTGTCACCTT TGAGGACTTT 
GACAGCTCCC ACGTCCAGCA ATTCTGGGAT GCCCTCAACA ACTTCACCAG CGAGGACCTC 
AGCCGCTTCC TCAAGTTCGT CACCGGCCGC AGCCGCCTCC CTGTTCAGCT CACCATCTAC 
CCAGACCGGT CCATCCCTGA GCGGTTGGAC ATGATGCCTG AGGCCTCCAC GTGCTCCTGC 
ACCTTGTTCT TGCCCAAATA CTCCTCGGTC AAGACGTGTG AGGAGCTGCT GCGCTACGCC 
GTGTATAACT GCATGTCCAT CGACACCGAC AAGGACAACT GGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025008850.1
CDS	222..2489
Translation	MELFPAHGLLEHLEEHGVCRELEEGWVLAQRFGDGGDKLVPVERIQQQQQTFGTDYKPAASWEQVVDLMYSMQLGEKPVPVEPDEAVVQKLRSMPPNWSYEQDMELGRFLYDVTERDEHCKDCVKDHLNGIEVSSQLDDFKVSHLTDNQGDTYWESSGSPGQHWVRLHMKKGSVIKKLWLMLNANINSYVPRRVAVYGGPANRLQHLRTVLINDRSSLLHPLLLQPWNSYQDVCILRDMKTHVPVLEIRILECREQGYNVRLRGIKFKSFWERDLNLNADMFQMAQLVRYPLLEGVDTDVLYRRAVVIQRFTQLLDSVLGYLIPISDESDSTFSVLRGMKPFLQLCKLSKTLVTHCLQSSESSPPCMLPKLLINRQLAREHRAHPELDPSGRNTVFTQVYENLKPSKTNNHLLNYRWPQTHSQWWECVFTTEGIIDNGGGFRDCLTDISEELCPSSGDVPVPLPFFVRTSNQGNSSSDTRDMYVPNPSCKDFPKYEWIGQLMGAALRSKEILVLALPSLVWKQLSGEEVIWSKDFAAVDVELVKLLQMLEEVDREAFNFMFGKELTYTTVRSDQRVVELIPKGSSTVVRFEDRKEFIRLVQKARLEESKEQVEAIRAGLLRVVPQAVLDLLTWQQLEKKVCGNPEVTVDELKKFVTFEDFDSSHVQQFWDALNNFTSEDLSRFLKFVTGRSRLPVQLTIYPDRSIPERLDMMPEASTCSCTLFLPKYSSVKTCEELLRYAVYNCMSIDTDKDNWD

Target ORF information:

RefSeq Version	XM_025153082.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus E3 ubiquitin-protein ligase HECTD3-like (HECTD3L), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025153082.1

ORF Insert Sequence:

ATGGAGCTTT TTCCGGCGCA CGGCCTGCTC GAGCACCTGG AGGAGCACGG GGTCTGCCGT 
GAGCTGGAGG AGGGCTGGGT CCTGGCGCAG CGGTTCGGAG ATGGGGGAGA TAAGTTGGTG 
CCGGTGGAGA GGATCCAGCA GCAACAGCAG ACCTTTGGAA CGGATTACAA ACCTGCGGCC 
AGCTGGGAAC AAGTGGTGGA CCTGATGTAC TCCATGCAGC TGGGAGAGAA GCCCGTGCCA 
GTCGAGCCGG ATGAGGCTGT GGTGCAGAAG CTTCGGTCGA TGCCCCCCAA TTGGAGCTAC 
GAGCAGGACA TGGAGCTGGG GCGCTTCCTG TACGACGTCA CTGAGAGGGA TGAACACTGC 
AAGGACTGCG TTAAGGACCA CCTCAATGGC ATAGAGGTCT CCTCACAACT GGATGACTTC 
AAAGTATCCC ACCTGACAGA CAACCAGGGA GACACCTACT GGGAGAGCAG CGGGTCCCCT 
GGGCAGCATT GGGTGCGGCT CCACATGAAG AAAGGCAGCG TCATCAAGAA GCTGTGGCTG 
ATGCTGAACG CAAACATCAA CTCCTACGTC CCCAGGAGGG TGGCTGTGTA CGGAGGGCCA 
GCCAACAGGC TGCAGCACCT GAGAACCGTC CTAATCAACG ACCGCTCCTC TCTGCTCCAT 
CCCCTCCTGC TGCAGCCCTG GAACAGCTAT CAAGATGTGT GCATCCTACG TGACATGAAG 
ACCCACGTGC CGGTCCTGGA GATCCGCATC TTGGAGTGCC GAGAACAAGG GTACAACGTC 
CGGCTGCGAG GGATTAAGTT CAAGTCCTTC TGGGAGAGGG ACCTGAACCT CAACGCAGAC 
ATGTTCCAGA TGGCCCAGCT GGTGCGCTAC CCACTGCTGG AAGGGGTGGA CACAGATGTG 
CTGTACCGCA GGGCTGTAGT CATCCAGAGG TTCACCCAGC TCCTGGACAG TGTCCTGGGG 
TACCTGATCC CCATCTCTGA TGAAAGTGAC AGCACCTTCA GTGTGCTCAG GGGCATGAAG 
CCATTCCTGC AGCTGTGCAA GCTGAGCAAA ACCCTCGTCA CCCATTGCCT GCAGAGCTCA 
GAGAGCAGCC CACCTTGCAT GCTGCCAAAG CTGCTCATCA ACCGGCAGCT GGCCCGTGAG 
CACCGGGCTC ACCCTGAGCT GGACCCTAGT GGCCGGAACA CAGTCTTCAC CCAGGTGTAT 
GAAAACCTGA AGCCTTCCAA AACCAACAAC CATCTCCTAA ACTACAGGTG GCCTCAGACC 
CACAGCCAGT GGTGGGAGTG TGTCTTCACC ACGGAGGGCA TCATCGATAA TGGCGGTGGT 
TTTCGGGACT GCTTGACAGA CATCTCAGAA GAGCTGTGTC CCAGCTCGGG CGATGTCCCC 
GTGCCTCTGC CCTTCTTTGT GCGCACATCC AACCAGGGTA ACAGCAGCAG CGACACCAGG 
GACATGTACG TCCCCAACCC CTCCTGCAAG GACTTCCCTA AGTATGAGTG GATCGGACAG 
CTCATGGGAG CAGCCCTGAG GAGCAAGGAG ATTCTGGTCC TGGCTCTGCC CAGTCTGGTG 
TGGAAGCAGC TGTCAGGGGA GGAGGTCATC TGGAGCAAGG ACTTTGCTGC TGTGGATGTG 
GAGCTGGTGA AGCTGCTGCA GATGCTGGAG GAAGTGGACA GGGAGGCCTT CAACTTCATG 
TTTGGCAAGG AGCTGACCTA CACAACGGTA CGGAGCGACC AGCGCGTGGT GGAGCTGATC 
CCAAAGGGCA GCAGCACTGT GGTGCGCTTT GAGGACCGCA AGGAGTTCAT CCGTCTGGTG 
CAGAAGGCAC GGCTGGAGGA GAGCAAGGAG CAGGTGGAGG CCATTCGTGC CGGGCTGCTC 
CGTGTGGTGC CTCAGGCTGT GCTGGACCTG CTCACCTGGC AGCAGCTGGA GAAGAAGGTC 
TGTGGGAACC CCGAGGTGAC GGTGGACGAG CTGAAGAAGT TTGTCACCTT TGAGGACTTT 
GACAGCTCCC ACGTCCAGCA ATTCTGGGAT GCCCTCAACA ACTTCACCAG CGAGGACCTC 
AGCCGCTTCC TCAAGTTCGT CACCGGCCGC AGCCGCCTCC CTGTTCAGCT CACCATCTAC 
CCAGACCGGT CCATCCCTGA GCGGTTGGAC ATGATGCCTG AGGCCTCCAC GTGCTCCTGC 
ACCTTGTTCT TGCCCAAATA CTCCTCGGTC AAGACGTGTG AGGAGCTGCT GCGCTACGCC 
GTGTATAACT GCATGTCCAT CGACACCGAC AAGGACAACT GGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC424588 ( XM_015291283.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015146769.1 Gallus gallus E3 ubiquitin-protein ligase HECTD3-like (LOC424588), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa39557
Clone ID Related Accession (Same CDS sequence)	XM_015291283.1 , XM_015291283.2
Accession Version	XM_015291283.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase HECTD3-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCGG GCGAGGAGGC CACGCACCAG GTGCTGGGCC GGCTGCGGTT CCTGCTGCAG 
TGCAGCGAGT GCTTCCGCCG CGCCCGGCCG CTGCCCGCCG CGCTGTGCTA CGTGCCCCGC 
GAGGTGCAGT ACAAGATCTG CAAGGACCCC TCGGCCGCCG CCTCGTCCCG CAGCCTGCTC 
AGCGTCTGGG ACGGCCCCGG GCCGGCGCGG GGCTCCAAGC GGGCCACCAT CGAGGTGCGG 
AAGGGCGGCT GTCTCCGTGC TACGGGAGAG GAGTACCGCA ACAGCGCGGG GCTGTGGGTG 
AAGCTCCGCA AGGAGCACCT GGAGGAGCAC GGGGTCTGCC GTGAGCTGGA GGAGGGCTGG 
GTCCTGGCGC AGCGGTTCGG AGATGGGGGA GATAAGTTGG TGCCGGTGGA GAGGATCCAG 
CAGCAACAGC AGACCTTTGG AACGGATTAC AAACCTGCGG CCAGCTGGGA ACAAGTGGTG 
GACCTGATGT ACTCCATGCA GCTGGGAGAG AAGCCCGTGC CAGTCGAGCC GGATGAGGCT 
GTGGTGCAGA AGCTTCGGTC GATGCCCCCC AATTGGAGCT ACGAGCAGGA CATGGAGCTG 
GGGCGCTTCC TGTACGACGT CACTGAGAGG GATGAACACT GCAAGGACTG CGTTAAGGAC 
CACCTCAATG GCATAGAGGT CTCCTCACAA CTGGATGACT TCAAAGTATC CCACCTGACA 
GACAACCAGG GAGACACCTA CTGGGAGAGC AGCGGGTCCC CTGGGCAGCA TTGGGTGCGG 
CTCCACATGA AGAAAGGCAG CGTCATCAAG AAGCTGTGGC TGATGCTGAA CGCAAACATC 
AACTCCTACG TCCCCAGGAG GGTGGCTGTG TACGGAGGGC CAGCCAACAG GCTGCAGCAC 
CTGAGAACCG TCCTAATCAA CGACCGCTCC TCTCTGCTCC ATCCCCTCCT GCTGCAGCCC 
TGGAACAGCT ATCAAGATGT GTGCATCCTA CGTGACATGA AGACCCACGT GCCGGTCCTG 
GAGATCCGCA TCTTGGAGTG CCGAGAACAA GGGTACAACG TCCGGCTGCG AGGGATTAAG 
TTCAAGTCCT TCTGGGAGAG GGACCTGAAC CTCAACGCAG ACATGTTCCA GATGGCCCAG 
CTGGTGCGCT ACCCACTGCT GGAAGGGGTG GACACAGATG TGCTGTACCG CAGGGCTGTA 
GTCATCCAGA GGTTCACCCA GCTCCTGGAC AGTGTCCTGG GGTACCTGAT CCCCATCTCT 
GATGAAAGTG ACAGCACCTT CAGTGTGCTC AGGGGCATGA AGCCATTCCT GCAGCTGTGC 
AAGCTGAGCA AAACCCTCGT CACCCATTGC CTGCAGAGCT CAGAGAGCAG CCCACCTTGC 
ATGCTGCCAA AGCTGCTCAT CAACCGGCAG CTGGCCCGTG AGCACCGGGC TCACCCTGAG 
CTGGACCCTA GTGGCCGGAA CACAGTCTTC ACCCAGGTGT ATGAAAACCT GAAGCCTTCC 
AAAACCAACA ACCATCTCCT AAACTACAGG TGGCCTCAGA CCCACAGCCA GTGGTGGGAG 
TGTGTCTTCA CCACGGAGGG CATCATCGAT AATGGCGGTG GTTTTCGGGA CTGCTTGACA 
GACATCTCAG AAGAGCTGTG TCCCAGCTCG GGCGATGTCC CCGTGCCTCT GCCCTTCTTT 
GTGCGCACAT CCAACCAGGG TAACAGCAGC AGCGACACCA GGGACATGTA CGTCCCCAAC 
CCCTCCTGCA AGGACTTCCC TAAGTATGAG TGGATCGGAC AGCTCATGGG AGCAGCCCTG 
AGGAGCAAGG AGATTCTGGT CCTGGCTCTG CCCAGTCTGG TGTGGAAGCA GCTGTCAGGG 
GAGGAGGTCA TCTGGAGCAA GGACTTTGCT GCTGTGGATG TGGAGCTGGT GAAGCTGCTG 
CAGATGCTGG AGGAAGTGGA CAGGGAGGCC TTCAACTTCA TGTTTGGCAA GGAGCTGACC 
TACACAACGG TACGGAGCGA CCAGCGCGTG GTGGAGCTGA TCCCAAAGGG CAGCAGCACT 
GTGGTGCGCT TTGAGGACCG CAAGGAGTTC ATCCGTCTGG TGCAGAAGGC ACGGCTGGAG 
GAGAGCAAGG AGCAGGTGGA GGCCATTCGT GCCGGGCTGC TCCGTGTGGT GCCTCAGGCT 
GTGCTGGACC TGCTCACCTG GCAGCAGCTG GAGAAGAAGG TCTGTGGGAA CCCCGAGGTG 
ACGGTGGACG AGCTGAAGAA GTTTGTCACC TTTGAGGACT TTGACAGCTC CCACGTCCAG 
CAATTCTGGG ATGCCCTCAA CAACTTCACC AGCGAGGACC TCAGCCGCTT CCTCAAGTTC 
GTCACCGGCC GCAGCCGCCT CCCTGTTCAG CTCACCATCT ACCCAGACCG GTCCATCCCT 
GAGCGGTTGG ACATGATGCC TGAGGCCTCC ACGTGCTCCT GCACCTTGTT CTTGCCCAAA 
TACTCCTCGG TCAAGACGTG TGAGGAGCTG CTGCGCTACG CCGTGTATAA CTGCATGTCC 
ATCGACACCG ACAAGGACAA CTGGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015146769.1
CDS	84..2633
Translation	MRAGEEATHQVLGRLRFLLQCSECFRRARPLPAALCYVPREVQYKICKDPSAAASSRSLLSVWDGPGPARGSKRATIEVRKGGCLRATGEEYRNSAGLWVKLRKEHLEEHGVCRELEEGWVLAQRFGDGGDKLVPVERIQQQQQTFGTDYKPAASWEQVVDLMYSMQLGEKPVPVEPDEAVVQKLRSMPPNWSYEQDMELGRFLYDVTERDEHCKDCVKDHLNGIEVSSQLDDFKVSHLTDNQGDTYWESSGSPGQHWVRLHMKKGSVIKKLWLMLNANINSYVPRRVAVYGGPANRLQHLRTVLINDRSSLLHPLLLQPWNSYQDVCILRDMKTHVPVLEIRILECREQGYNVRLRGIKFKSFWERDLNLNADMFQMAQLVRYPLLEGVDTDVLYRRAVVIQRFTQLLDSVLGYLIPISDESDSTFSVLRGMKPFLQLCKLSKTLVTHCLQSSESSPPCMLPKLLINRQLAREHRAHPELDPSGRNTVFTQVYENLKPSKTNNHLLNYRWPQTHSQWWECVFTTEGIIDNGGGFRDCLTDISEELCPSSGDVPVPLPFFVRTSNQGNSSSDTRDMYVPNPSCKDFPKYEWIGQLMGAALRSKEILVLALPSLVWKQLSGEEVIWSKDFAAVDVELVKLLQMLEEVDREAFNFMFGKELTYTTVRSDQRVVELIPKGSSTVVRFEDRKEFIRLVQKARLEESKEQVEAIRAGLLRVVPQAVLDLLTWQQLEKKVCGNPEVTVDELKKFVTFEDFDSSHVQQFWDALNNFTSEDLSRFLKFVTGRSRLPVQLTIYPDRSIPERLDMMPEASTCSCTLFLPKYSSVKTCEELLRYAVYNCMSIDTDKDNWD

Target ORF information:

RefSeq Version	XM_015291283.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus E3 ubiquitin-protein ligase HECTD3-like (LOC424588), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291283.1

ORF Insert Sequence:

ATGCGGGCGG GCGAGGAGGC CACGCACCAG GTGCTGGGCC GGCTGCGGTT CCTGCTGCAG 
TGCAGCGAGT GCTTCCGCCG CGCCCGGCCG CTGCCCGCCG CGCTGTGCTA CGTGCCCCGC 
GAGGTGCAGT ACAAGATCTG CAAGGACCCC TCGGCCGCCG CCTCGTCCCG CAGCCTGCTC 
AGCGTCTGGG ACGGCCCCGG GCCGGCGCGG GGCTCCAAGC GGGCCACCAT CGAGGTGCGG 
AAGGGCGGCT GTCTCCGTGC TACGGGAGAG GAGTACCGCA ACAGCGCGGG GCTGTGGGTG 
AAGCTCCGCA AGGAGCACCT GGAGGAGCAC GGGGTCTGCC GTGAGCTGGA GGAGGGCTGG 
GTCCTGGCGC AGCGGTTCGG AGATGGGGGA GATAAGTTGG TGCCGGTGGA GAGGATCCAG 
CAGCAACAGC AGACCTTTGG AACGGATTAC AAACCTGCGG CCAGCTGGGA ACAAGTGGTG 
GACCTGATGT ACTCCATGCA GCTGGGAGAG AAGCCCGTGC CAGTCGAGCC GGATGAGGCT 
GTGGTGCAGA AGCTTCGGTC GATGCCCCCC AATTGGAGCT ACGAGCAGGA CATGGAGCTG 
GGGCGCTTCC TGTACGACGT CACTGAGAGG GATGAACACT GCAAGGACTG CGTTAAGGAC 
CACCTCAATG GCATAGAGGT CTCCTCACAA CTGGATGACT TCAAAGTATC CCACCTGACA 
GACAACCAGG GAGACACCTA CTGGGAGAGC AGCGGGTCCC CTGGGCAGCA TTGGGTGCGG 
CTCCACATGA AGAAAGGCAG CGTCATCAAG AAGCTGTGGC TGATGCTGAA CGCAAACATC 
AACTCCTACG TCCCCAGGAG GGTGGCTGTG TACGGAGGGC CAGCCAACAG GCTGCAGCAC 
CTGAGAACCG TCCTAATCAA CGACCGCTCC TCTCTGCTCC ATCCCCTCCT GCTGCAGCCC 
TGGAACAGCT ATCAAGATGT GTGCATCCTA CGTGACATGA AGACCCACGT GCCGGTCCTG 
GAGATCCGCA TCTTGGAGTG CCGAGAACAA GGGTACAACG TCCGGCTGCG AGGGATTAAG 
TTCAAGTCCT TCTGGGAGAG GGACCTGAAC CTCAACGCAG ACATGTTCCA GATGGCCCAG 
CTGGTGCGCT ACCCACTGCT GGAAGGGGTG GACACAGATG TGCTGTACCG CAGGGCTGTA 
GTCATCCAGA GGTTCACCCA GCTCCTGGAC AGTGTCCTGG GGTACCTGAT CCCCATCTCT 
GATGAAAGTG ACAGCACCTT CAGTGTGCTC AGGGGCATGA AGCCATTCCT GCAGCTGTGC 
AAGCTGAGCA AAACCCTCGT CACCCATTGC CTGCAGAGCT CAGAGAGCAG CCCACCTTGC 
ATGCTGCCAA AGCTGCTCAT CAACCGGCAG CTGGCCCGTG AGCACCGGGC TCACCCTGAG 
CTGGACCCTA GTGGCCGGAA CACAGTCTTC ACCCAGGTGT ATGAAAACCT GAAGCCTTCC 
AAAACCAACA ACCATCTCCT AAACTACAGG TGGCCTCAGA CCCACAGCCA GTGGTGGGAG 
TGTGTCTTCA CCACGGAGGG CATCATCGAT AATGGCGGTG GTTTTCGGGA CTGCTTGACA 
GACATCTCAG AAGAGCTGTG TCCCAGCTCG GGCGATGTCC CCGTGCCTCT GCCCTTCTTT 
GTGCGCACAT CCAACCAGGG TAACAGCAGC AGCGACACCA GGGACATGTA CGTCCCCAAC 
CCCTCCTGCA AGGACTTCCC TAAGTATGAG TGGATCGGAC AGCTCATGGG AGCAGCCCTG 
AGGAGCAAGG AGATTCTGGT CCTGGCTCTG CCCAGTCTGG TGTGGAAGCA GCTGTCAGGG 
GAGGAGGTCA TCTGGAGCAA GGACTTTGCT GCTGTGGATG TGGAGCTGGT GAAGCTGCTG 
CAGATGCTGG AGGAAGTGGA CAGGGAGGCC TTCAACTTCA TGTTTGGCAA GGAGCTGACC 
TACACAACGG TACGGAGCGA CCAGCGCGTG GTGGAGCTGA TCCCAAAGGG CAGCAGCACT 
GTGGTGCGCT TTGAGGACCG CAAGGAGTTC ATCCGTCTGG TGCAGAAGGC ACGGCTGGAG 
GAGAGCAAGG AGCAGGTGGA GGCCATTCGT GCCGGGCTGC TCCGTGTGGT GCCTCAGGCT 
GTGCTGGACC TGCTCACCTG GCAGCAGCTG GAGAAGAAGG TCTGTGGGAA CCCCGAGGTG 
ACGGTGGACG AGCTGAAGAA GTTTGTCACC TTTGAGGACT TTGACAGCTC CCACGTCCAG 
CAATTCTGGG ATGCCCTCAA CAACTTCACC AGCGAGGACC TCAGCCGCTT CCTCAAGTTC 
GTCACCGGCC GCAGCCGCCT CCCTGTTCAG CTCACCATCT ACCCAGACCG GTCCATCCCT 
GAGCGGTTGG ACATGATGCC TGAGGCCTCC ACGTGCTCCT GCACCTTGTT CTTGCCCAAA 
TACTCCTCGG TCAAGACGTG TGAGGAGCTG CTGCGCTACG CCGTGTATAA CTGCATGTCC 
ATCGACACCG ACAAGGACAA CTGGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC424588 ( XM_015291283.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015146769.1 Gallus gallus E3 ubiquitin-protein ligase HECTD3-like (HECTD3L), transcript variant X1, mRNA.

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CloneID	OGa39557
Clone ID Related Accession (Same CDS sequence)	XM_015291283.1 , XM_015291283.2
Accession Version	XM_015291283.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase HECTD3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291283.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCGG GCGAGGAGGC CACGCACCAG GTGCTGGGCC GGCTGCGGTT CCTGCTGCAG 
TGCAGCGAGT GCTTCCGCCG CGCCCGGCCG CTGCCCGCCG CGCTGTGCTA CGTGCCCCGC 
GAGGTGCAGT ACAAGATCTG CAAGGACCCC TCGGCCGCCG CCTCGTCCCG CAGCCTGCTC 
AGCGTCTGGG ACGGCCCCGG GCCGGCGCGG GGCTCCAAGC GGGCCACCAT CGAGGTGCGG 
AAGGGCGGCT GTCTCCGTGC TACGGGAGAG GAGTACCGCA ACAGCGCGGG GCTGTGGGTG 
AAGCTCCGCA AGGAGCACCT GGAGGAGCAC GGGGTCTGCC GTGAGCTGGA GGAGGGCTGG 
GTCCTGGCGC AGCGGTTCGG AGATGGGGGA GATAAGTTGG TGCCGGTGGA GAGGATCCAG 
CAGCAACAGC AGACCTTTGG AACGGATTAC AAACCTGCGG CCAGCTGGGA ACAAGTGGTG 
GACCTGATGT ACTCCATGCA GCTGGGAGAG AAGCCCGTGC CAGTCGAGCC GGATGAGGCT 
GTGGTGCAGA AGCTTCGGTC GATGCCCCCC AATTGGAGCT ACGAGCAGGA CATGGAGCTG 
GGGCGCTTCC TGTACGACGT CACTGAGAGG GATGAACACT GCAAGGACTG CGTTAAGGAC 
CACCTCAATG GCATAGAGGT CTCCTCACAA CTGGATGACT TCAAAGTATC CCACCTGACA 
GACAACCAGG GAGACACCTA CTGGGAGAGC AGCGGGTCCC CTGGGCAGCA TTGGGTGCGG 
CTCCACATGA AGAAAGGCAG CGTCATCAAG AAGCTGTGGC TGATGCTGAA CGCAAACATC 
AACTCCTACG TCCCCAGGAG GGTGGCTGTG TACGGAGGGC CAGCCAACAG GCTGCAGCAC 
CTGAGAACCG TCCTAATCAA CGACCGCTCC TCTCTGCTCC ATCCCCTCCT GCTGCAGCCC 
TGGAACAGCT ATCAAGATGT GTGCATCCTA CGTGACATGA AGACCCACGT GCCGGTCCTG 
GAGATCCGCA TCTTGGAGTG CCGAGAACAA GGGTACAACG TCCGGCTGCG AGGGATTAAG 
TTCAAGTCCT TCTGGGAGAG GGACCTGAAC CTCAACGCAG ACATGTTCCA GATGGCCCAG 
CTGGTGCGCT ACCCACTGCT GGAAGGGGTG GACACAGATG TGCTGTACCG CAGGGCTGTA 
GTCATCCAGA GGTTCACCCA GCTCCTGGAC AGTGTCCTGG GGTACCTGAT CCCCATCTCT 
GATGAAAGTG ACAGCACCTT CAGTGTGCTC AGGGGCATGA AGCCATTCCT GCAGCTGTGC 
AAGCTGAGCA AAACCCTCGT CACCCATTGC CTGCAGAGCT CAGAGAGCAG CCCACCTTGC 
ATGCTGCCAA AGCTGCTCAT CAACCGGCAG CTGGCCCGTG AGCACCGGGC TCACCCTGAG 
CTGGACCCTA GTGGCCGGAA CACAGTCTTC ACCCAGGTGT ATGAAAACCT GAAGCCTTCC 
AAAACCAACA ACCATCTCCT AAACTACAGG TGGCCTCAGA CCCACAGCCA GTGGTGGGAG 
TGTGTCTTCA CCACGGAGGG CATCATCGAT AATGGCGGTG GTTTTCGGGA CTGCTTGACA 
GACATCTCAG AAGAGCTGTG TCCCAGCTCG GGCGATGTCC CCGTGCCTCT GCCCTTCTTT 
GTGCGCACAT CCAACCAGGG TAACAGCAGC AGCGACACCA GGGACATGTA CGTCCCCAAC 
CCCTCCTGCA AGGACTTCCC TAAGTATGAG TGGATCGGAC AGCTCATGGG AGCAGCCCTG 
AGGAGCAAGG AGATTCTGGT CCTGGCTCTG CCCAGTCTGG TGTGGAAGCA GCTGTCAGGG 
GAGGAGGTCA TCTGGAGCAA GGACTTTGCT GCTGTGGATG TGGAGCTGGT GAAGCTGCTG 
CAGATGCTGG AGGAAGTGGA CAGGGAGGCC TTCAACTTCA TGTTTGGCAA GGAGCTGACC 
TACACAACGG TACGGAGCGA CCAGCGCGTG GTGGAGCTGA TCCCAAAGGG CAGCAGCACT 
GTGGTGCGCT TTGAGGACCG CAAGGAGTTC ATCCGTCTGG TGCAGAAGGC ACGGCTGGAG 
GAGAGCAAGG AGCAGGTGGA GGCCATTCGT GCCGGGCTGC TCCGTGTGGT GCCTCAGGCT 
GTGCTGGACC TGCTCACCTG GCAGCAGCTG GAGAAGAAGG TCTGTGGGAA CCCCGAGGTG 
ACGGTGGACG AGCTGAAGAA GTTTGTCACC TTTGAGGACT TTGACAGCTC CCACGTCCAG 
CAATTCTGGG ATGCCCTCAA CAACTTCACC AGCGAGGACC TCAGCCGCTT CCTCAAGTTC 
GTCACCGGCC GCAGCCGCCT CCCTGTTCAG CTCACCATCT ACCCAGACCG GTCCATCCCT 
GAGCGGTTGG ACATGATGCC TGAGGCCTCC ACGTGCTCCT GCACCTTGTT CTTGCCCAAA 
TACTCCTCGG TCAAGACGTG TGAGGAGCTG CTGCGCTACG CCGTGTATAA CTGCATGTCC 
ATCGACACCG ACAAGGACAA CTGGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015146769.1
CDS	161..2710
Translation	MRAGEEATHQVLGRLRFLLQCSECFRRARPLPAALCYVPREVQYKICKDPSAAASSRSLLSVWDGPGPARGSKRATIEVRKGGCLRATGEEYRNSAGLWVKLRKEHLEEHGVCRELEEGWVLAQRFGDGGDKLVPVERIQQQQQTFGTDYKPAASWEQVVDLMYSMQLGEKPVPVEPDEAVVQKLRSMPPNWSYEQDMELGRFLYDVTERDEHCKDCVKDHLNGIEVSSQLDDFKVSHLTDNQGDTYWESSGSPGQHWVRLHMKKGSVIKKLWLMLNANINSYVPRRVAVYGGPANRLQHLRTVLINDRSSLLHPLLLQPWNSYQDVCILRDMKTHVPVLEIRILECREQGYNVRLRGIKFKSFWERDLNLNADMFQMAQLVRYPLLEGVDTDVLYRRAVVIQRFTQLLDSVLGYLIPISDESDSTFSVLRGMKPFLQLCKLSKTLVTHCLQSSESSPPCMLPKLLINRQLAREHRAHPELDPSGRNTVFTQVYENLKPSKTNNHLLNYRWPQTHSQWWECVFTTEGIIDNGGGFRDCLTDISEELCPSSGDVPVPLPFFVRTSNQGNSSSDTRDMYVPNPSCKDFPKYEWIGQLMGAALRSKEILVLALPSLVWKQLSGEEVIWSKDFAAVDVELVKLLQMLEEVDREAFNFMFGKELTYTTVRSDQRVVELIPKGSSTVVRFEDRKEFIRLVQKARLEESKEQVEAIRAGLLRVVPQAVLDLLTWQQLEKKVCGNPEVTVDELKKFVTFEDFDSSHVQQFWDALNNFTSEDLSRFLKFVTGRSRLPVQLTIYPDRSIPERLDMMPEASTCSCTLFLPKYSSVKTCEELLRYAVYNCMSIDTDKDNWD

Target ORF information:

RefSeq Version	XM_015291283.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus E3 ubiquitin-protein ligase HECTD3-like (HECTD3L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291283.2

ORF Insert Sequence:

ATGCGGGCGG GCGAGGAGGC CACGCACCAG GTGCTGGGCC GGCTGCGGTT CCTGCTGCAG 
TGCAGCGAGT GCTTCCGCCG CGCCCGGCCG CTGCCCGCCG CGCTGTGCTA CGTGCCCCGC 
GAGGTGCAGT ACAAGATCTG CAAGGACCCC TCGGCCGCCG CCTCGTCCCG CAGCCTGCTC 
AGCGTCTGGG ACGGCCCCGG GCCGGCGCGG GGCTCCAAGC GGGCCACCAT CGAGGTGCGG 
AAGGGCGGCT GTCTCCGTGC TACGGGAGAG GAGTACCGCA ACAGCGCGGG GCTGTGGGTG 
AAGCTCCGCA AGGAGCACCT GGAGGAGCAC GGGGTCTGCC GTGAGCTGGA GGAGGGCTGG 
GTCCTGGCGC AGCGGTTCGG AGATGGGGGA GATAAGTTGG TGCCGGTGGA GAGGATCCAG 
CAGCAACAGC AGACCTTTGG AACGGATTAC AAACCTGCGG CCAGCTGGGA ACAAGTGGTG 
GACCTGATGT ACTCCATGCA GCTGGGAGAG AAGCCCGTGC CAGTCGAGCC GGATGAGGCT 
GTGGTGCAGA AGCTTCGGTC GATGCCCCCC AATTGGAGCT ACGAGCAGGA CATGGAGCTG 
GGGCGCTTCC TGTACGACGT CACTGAGAGG GATGAACACT GCAAGGACTG CGTTAAGGAC 
CACCTCAATG GCATAGAGGT CTCCTCACAA CTGGATGACT TCAAAGTATC CCACCTGACA 
GACAACCAGG GAGACACCTA CTGGGAGAGC AGCGGGTCCC CTGGGCAGCA TTGGGTGCGG 
CTCCACATGA AGAAAGGCAG CGTCATCAAG AAGCTGTGGC TGATGCTGAA CGCAAACATC 
AACTCCTACG TCCCCAGGAG GGTGGCTGTG TACGGAGGGC CAGCCAACAG GCTGCAGCAC 
CTGAGAACCG TCCTAATCAA CGACCGCTCC TCTCTGCTCC ATCCCCTCCT GCTGCAGCCC 
TGGAACAGCT ATCAAGATGT GTGCATCCTA CGTGACATGA AGACCCACGT GCCGGTCCTG 
GAGATCCGCA TCTTGGAGTG CCGAGAACAA GGGTACAACG TCCGGCTGCG AGGGATTAAG 
TTCAAGTCCT TCTGGGAGAG GGACCTGAAC CTCAACGCAG ACATGTTCCA GATGGCCCAG 
CTGGTGCGCT ACCCACTGCT GGAAGGGGTG GACACAGATG TGCTGTACCG CAGGGCTGTA 
GTCATCCAGA GGTTCACCCA GCTCCTGGAC AGTGTCCTGG GGTACCTGAT CCCCATCTCT 
GATGAAAGTG ACAGCACCTT CAGTGTGCTC AGGGGCATGA AGCCATTCCT GCAGCTGTGC 
AAGCTGAGCA AAACCCTCGT CACCCATTGC CTGCAGAGCT CAGAGAGCAG CCCACCTTGC 
ATGCTGCCAA AGCTGCTCAT CAACCGGCAG CTGGCCCGTG AGCACCGGGC TCACCCTGAG 
CTGGACCCTA GTGGCCGGAA CACAGTCTTC ACCCAGGTGT ATGAAAACCT GAAGCCTTCC 
AAAACCAACA ACCATCTCCT AAACTACAGG TGGCCTCAGA CCCACAGCCA GTGGTGGGAG 
TGTGTCTTCA CCACGGAGGG CATCATCGAT AATGGCGGTG GTTTTCGGGA CTGCTTGACA 
GACATCTCAG AAGAGCTGTG TCCCAGCTCG GGCGATGTCC CCGTGCCTCT GCCCTTCTTT 
GTGCGCACAT CCAACCAGGG TAACAGCAGC AGCGACACCA GGGACATGTA CGTCCCCAAC 
CCCTCCTGCA AGGACTTCCC TAAGTATGAG TGGATCGGAC AGCTCATGGG AGCAGCCCTG 
AGGAGCAAGG AGATTCTGGT CCTGGCTCTG CCCAGTCTGG TGTGGAAGCA GCTGTCAGGG 
GAGGAGGTCA TCTGGAGCAA GGACTTTGCT GCTGTGGATG TGGAGCTGGT GAAGCTGCTG 
CAGATGCTGG AGGAAGTGGA CAGGGAGGCC TTCAACTTCA TGTTTGGCAA GGAGCTGACC 
TACACAACGG TACGGAGCGA CCAGCGCGTG GTGGAGCTGA TCCCAAAGGG CAGCAGCACT 
GTGGTGCGCT TTGAGGACCG CAAGGAGTTC ATCCGTCTGG TGCAGAAGGC ACGGCTGGAG 
GAGAGCAAGG AGCAGGTGGA GGCCATTCGT GCCGGGCTGC TCCGTGTGGT GCCTCAGGCT 
GTGCTGGACC TGCTCACCTG GCAGCAGCTG GAGAAGAAGG TCTGTGGGAA CCCCGAGGTG 
ACGGTGGACG AGCTGAAGAA GTTTGTCACC TTTGAGGACT TTGACAGCTC CCACGTCCAG 
CAATTCTGGG ATGCCCTCAA CAACTTCACC AGCGAGGACC TCAGCCGCTT CCTCAAGTTC 
GTCACCGGCC GCAGCCGCCT CCCTGTTCAG CTCACCATCT ACCCAGACCG GTCCATCCCT 
GAGCGGTTGG ACATGATGCC TGAGGCCTCC ACGTGCTCCT GCACCTTGTT CTTGCCCAAA 
TACTCCTCGG TCAAGACGTG TGAGGAGCTG CTGCGCTACG CCGTGTATAA CTGCATGTCC 
ATCGACACCG ACAAGGACAA CTGGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC424588 ( XM_422428.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_422428.3 Gallus gallus E3 ubiquitin-protein ligase HECTD3-like (LOC424588), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa39558
Clone ID Related Accession (Same CDS sequence)	XM_422428.5 , XM_422428.6
Accession Version	XM_422428.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase HECTD3-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_422428.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCGG GCGAGGAGGC CACGCACCAG GTGCTGGGCC GGCTGCGGTT CCTGCTGCAG 
TGCAGCGAGT GCTTCCGCCG CGCCCGGCCG CTGCCCGCCG CGCTGTGCTA CGTGCCCCGC 
GAGGTGCAGT ACAAGATCTG CAAGGACCCC TCGGCCGCCG CCTCGTCCCG CAGCCTGCTC 
AGCGTCTGGG ACGGCCCCGG GCCGGCGCGG GGCTCCAAGC GGGCCACCAT CGAGGTGCGG 
AAGGGCGGCT GTCTCCGTGC TACGGGAGAG GAGTACCGCA ACAGCGCGGG GCTGTGGGTG 
AAGCTCCGCA AGGAGCACCT GGAGGAGCAC GGGGTCTGCC GTGAGCTGGA GGAGGGCTGG 
GTCCTGGCGC AGCGGTTCGG AGATGGGGGA GATAAGTTGG TGCCGGTGGA GAGGATCCAG 
CAGCAACAGC AGACCTTTGG AACGGATTAC AAACCTGCGG CCAGCTGGGA ACAAGTGGTG 
GACCTGATGT ACTCCATGCA GCTGGGAGAG AAGCCCGTGC CAGTCGAGCC GGATGAGGCT 
GTGGTGCAGA AGCTTCGGTC GATGCCCCCC AATTGGAGCT ACGAGCAGGA CATGGAGCTG 
GGGCGCTTCC TGTACGACGT CACTGAGAGG GATGAACACT GCAAGGACTG CGTTAAGGAC 
CACCTCAATG GCATAGAGGT CTCCTCACAA CTGGATGACT TCAAAGTATC CCACCTGACA 
GACAACCAGG GAGACACCTA CTGGGAGAGC AGCGGGTCCC CTGGGCAGCA TTGGGTGCGG 
CTCCACATGA AGAAAGGCAG CGTCATCAAG AAGCTGTGGC TGATGCTGAA CGCAAACATC 
AACTCCTACG TCCCCAGGAG GGTGGCTGTG TACGGAGGGC CAGCCAACAG GCTGCAGCAC 
CTGAGAACCG TCCTAATCAA CGAGAACAGC TATCAAGATG TGTGCATCCT ACGTGACATG 
AAGACCCACG TGCCGGTCCT GGAGATCCGC ATCTTGGAGT GCCGAGAACA AGGGTACAAC 
GTCCGGCTGC GAGGGATTAA GTTCAAGTCC TTCTGGGAGA GGGACCTGAA CCTCAACGCA 
GACATGTTCC AGATGGCCCA GCTGGTGCGC TACCCACTGC TGGAAGGGGT GGACACAGAT 
GTGCTGTACC GCAGGGCTGT AGTCATCCAG AGGTTCACCC AGCTCCTGGA CAGTGTCCTG 
GGGTACCTGA TCCCCATCTC TGATGAAAGT GACAGCACCT TCAGTGTGCT CAGGGGCATG 
AAGCCATTCC TGCAGCTGTG CAAGCTGAGC AAAACCCTCG TCACCCATTG CCTGCAGAGC 
TCAGAGAGCA GCCCACCTTG CATGCTGCCA AAGCTGCTCA TCAACCGGCA GCTGGCCCGT 
GAGCACCGGG CTCACCCTGA GCTGGACCCT AGTGGCCGGA ACACAGTCTT CACCCAGGTG 
TATGAAAACC TGAAGCCTTC CAAAACCAAC AACCATCTCC TAAACTACAG GTGGCCTCAG 
ACCCACAGCC AGTGGTGGGA GTGTGTCTTC ACCACGGAGG GCATCATCGA TAATGGCGGT 
GGTTTTCGGG ACTGCTTGAC AGACATCTCA GAAGAGCTGT GTCCCAGCTC GGGCGATGTC 
CCCGTGCCTC TGCCCTTCTT TGTGCGCACA TCCAACCAGG GTAACAGCAG CAGCGACACC 
AGGGACATGT ACGTCCCCAA CCCCTCCTGC AAGGACTTCC CTAAGTATGA GTGGATCGGA 
CAGCTCATGG GAGCAGCCCT GAGGAGCAAG GAGATTCTGG TCCTGGCTCT GCCCAGTCTG 
GTGTGGAAGC AGCTGTCAGG GGAGGAGGTC ATCTGGAGCA AGGACTTTGC TGCTGTGGAT 
GTGGAGCTGG TGAAGCTGCT GCAGATGCTG GAGGAAGTGG ACAGGGAGGC CTTCAACTTC 
ATGTTTGGCA AGGAGCTGAC CTACACAACG GTACGGAGCG ACCAGCGCGT GGTGGAGCTG 
ATCCCAAAGG GCAGCAGCAC TGTGGTGCGC TTTGAGGACC GCAAGGAGTT CATCCGTCTG 
GTGCAGAAGG CACGGCTGGA GGAGAGCAAG GAGCAGGTGG AGGCCATTCG TGCCGGGCTG 
CTCCGTGTGG TGCCTCAGGC TGTGCTGGAC CTGCTCACCT GGCAGCAGCT GGAGAAGAAG 
GTCTGTGGGA ACCCCGAGGT GACGGTGGAC GAGCTGAAGA AGTTTGTCAC CTTTGAGGAC 
TTTGACAGCT CCCACGTCCA GCAATTCTGG GATGCCCTCA ACAACTTCAC CAGCGAGGAC 
CTCAGCCGCT TCCTCAAGTT CGTCACCGGC CGCAGCCGCC TCCCTGTTCA GCTCACCATC 
TACCCAGACC GGTCCATCCC TGAGCGGTTG GACATGATGC CTGAGGCCTC CACGTGCTCC 
TGCACCTTGT TCTTGCCCAA ATACTCCTCG GTCAAGACGT GTGAGGAGCT GCTGCGCTAC 
GCCGTGTATA ACTGCATGTC CATCGACACC GACAAGGACA ACTGGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_422428.3
CDS	84..2594
Translation	MRAGEEATHQVLGRLRFLLQCSECFRRARPLPAALCYVPREVQYKICKDPSAAASSRSLLSVWDGPGPARGSKRATIEVRKGGCLRATGEEYRNSAGLWVKLRKEHLEEHGVCRELEEGWVLAQRFGDGGDKLVPVERIQQQQQTFGTDYKPAASWEQVVDLMYSMQLGEKPVPVEPDEAVVQKLRSMPPNWSYEQDMELGRFLYDVTERDEHCKDCVKDHLNGIEVSSQLDDFKVSHLTDNQGDTYWESSGSPGQHWVRLHMKKGSVIKKLWLMLNANINSYVPRRVAVYGGPANRLQHLRTVLINENSYQDVCILRDMKTHVPVLEIRILECREQGYNVRLRGIKFKSFWERDLNLNADMFQMAQLVRYPLLEGVDTDVLYRRAVVIQRFTQLLDSVLGYLIPISDESDSTFSVLRGMKPFLQLCKLSKTLVTHCLQSSESSPPCMLPKLLINRQLAREHRAHPELDPSGRNTVFTQVYENLKPSKTNNHLLNYRWPQTHSQWWECVFTTEGIIDNGGGFRDCLTDISEELCPSSGDVPVPLPFFVRTSNQGNSSSDTRDMYVPNPSCKDFPKYEWIGQLMGAALRSKEILVLALPSLVWKQLSGEEVIWSKDFAAVDVELVKLLQMLEEVDREAFNFMFGKELTYTTVRSDQRVVELIPKGSSTVVRFEDRKEFIRLVQKARLEESKEQVEAIRAGLLRVVPQAVLDLLTWQQLEKKVCGNPEVTVDELKKFVTFEDFDSSHVQQFWDALNNFTSEDLSRFLKFVTGRSRLPVQLTIYPDRSIPERLDMMPEASTCSCTLFLPKYSSVKTCEELLRYAVYNCMSIDTDKDNWD

Target ORF information:

RefSeq Version	XM_422428.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus E3 ubiquitin-protein ligase HECTD3-like (LOC424588), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_422428.5

ORF Insert Sequence:

ATGCGGGCGG GCGAGGAGGC CACGCACCAG GTGCTGGGCC GGCTGCGGTT CCTGCTGCAG 
TGCAGCGAGT GCTTCCGCCG CGCCCGGCCG CTGCCCGCCG CGCTGTGCTA CGTGCCCCGC 
GAGGTGCAGT ACAAGATCTG CAAGGACCCC TCGGCCGCCG CCTCGTCCCG CAGCCTGCTC 
AGCGTCTGGG ACGGCCCCGG GCCGGCGCGG GGCTCCAAGC GGGCCACCAT CGAGGTGCGG 
AAGGGCGGCT GTCTCCGTGC TACGGGAGAG GAGTACCGCA ACAGCGCGGG GCTGTGGGTG 
AAGCTCCGCA AGGAGCACCT GGAGGAGCAC GGGGTCTGCC GTGAGCTGGA GGAGGGCTGG 
GTCCTGGCGC AGCGGTTCGG AGATGGGGGA GATAAGTTGG TGCCGGTGGA GAGGATCCAG 
CAGCAACAGC AGACCTTTGG AACGGATTAC AAACCTGCGG CCAGCTGGGA ACAAGTGGTG 
GACCTGATGT ACTCCATGCA GCTGGGAGAG AAGCCCGTGC CAGTCGAGCC GGATGAGGCT 
GTGGTGCAGA AGCTTCGGTC GATGCCCCCC AATTGGAGCT ACGAGCAGGA CATGGAGCTG 
GGGCGCTTCC TGTACGACGT CACTGAGAGG GATGAACACT GCAAGGACTG CGTTAAGGAC 
CACCTCAATG GCATAGAGGT CTCCTCACAA CTGGATGACT TCAAAGTATC CCACCTGACA 
GACAACCAGG GAGACACCTA CTGGGAGAGC AGCGGGTCCC CTGGGCAGCA TTGGGTGCGG 
CTCCACATGA AGAAAGGCAG CGTCATCAAG AAGCTGTGGC TGATGCTGAA CGCAAACATC 
AACTCCTACG TCCCCAGGAG GGTGGCTGTG TACGGAGGGC CAGCCAACAG GCTGCAGCAC 
CTGAGAACCG TCCTAATCAA CGAGAACAGC TATCAAGATG TGTGCATCCT ACGTGACATG 
AAGACCCACG TGCCGGTCCT GGAGATCCGC ATCTTGGAGT GCCGAGAACA AGGGTACAAC 
GTCCGGCTGC GAGGGATTAA GTTCAAGTCC TTCTGGGAGA GGGACCTGAA CCTCAACGCA 
GACATGTTCC AGATGGCCCA GCTGGTGCGC TACCCACTGC TGGAAGGGGT GGACACAGAT 
GTGCTGTACC GCAGGGCTGT AGTCATCCAG AGGTTCACCC AGCTCCTGGA CAGTGTCCTG 
GGGTACCTGA TCCCCATCTC TGATGAAAGT GACAGCACCT TCAGTGTGCT CAGGGGCATG 
AAGCCATTCC TGCAGCTGTG CAAGCTGAGC AAAACCCTCG TCACCCATTG CCTGCAGAGC 
TCAGAGAGCA GCCCACCTTG CATGCTGCCA AAGCTGCTCA TCAACCGGCA GCTGGCCCGT 
GAGCACCGGG CTCACCCTGA GCTGGACCCT AGTGGCCGGA ACACAGTCTT CACCCAGGTG 
TATGAAAACC TGAAGCCTTC CAAAACCAAC AACCATCTCC TAAACTACAG GTGGCCTCAG 
ACCCACAGCC AGTGGTGGGA GTGTGTCTTC ACCACGGAGG GCATCATCGA TAATGGCGGT 
GGTTTTCGGG ACTGCTTGAC AGACATCTCA GAAGAGCTGT GTCCCAGCTC GGGCGATGTC 
CCCGTGCCTC TGCCCTTCTT TGTGCGCACA TCCAACCAGG GTAACAGCAG CAGCGACACC 
AGGGACATGT ACGTCCCCAA CCCCTCCTGC AAGGACTTCC CTAAGTATGA GTGGATCGGA 
CAGCTCATGG GAGCAGCCCT GAGGAGCAAG GAGATTCTGG TCCTGGCTCT GCCCAGTCTG 
GTGTGGAAGC AGCTGTCAGG GGAGGAGGTC ATCTGGAGCA AGGACTTTGC TGCTGTGGAT 
GTGGAGCTGG TGAAGCTGCT GCAGATGCTG GAGGAAGTGG ACAGGGAGGC CTTCAACTTC 
ATGTTTGGCA AGGAGCTGAC CTACACAACG GTACGGAGCG ACCAGCGCGT GGTGGAGCTG 
ATCCCAAAGG GCAGCAGCAC TGTGGTGCGC TTTGAGGACC GCAAGGAGTT CATCCGTCTG 
GTGCAGAAGG CACGGCTGGA GGAGAGCAAG GAGCAGGTGG AGGCCATTCG TGCCGGGCTG 
CTCCGTGTGG TGCCTCAGGC TGTGCTGGAC CTGCTCACCT GGCAGCAGCT GGAGAAGAAG 
GTCTGTGGGA ACCCCGAGGT GACGGTGGAC GAGCTGAAGA AGTTTGTCAC CTTTGAGGAC 
TTTGACAGCT CCCACGTCCA GCAATTCTGG GATGCCCTCA ACAACTTCAC CAGCGAGGAC 
CTCAGCCGCT TCCTCAAGTT CGTCACCGGC CGCAGCCGCC TCCCTGTTCA GCTCACCATC 
TACCCAGACC GGTCCATCCC TGAGCGGTTG GACATGATGC CTGAGGCCTC CACGTGCTCC 
TGCACCTTGT TCTTGCCCAA ATACTCCTCG GTCAAGACGT GTGAGGAGCT GCTGCGCTAC 
GCCGTGTATA ACTGCATGTC CATCGACACC GACAAGGACA ACTGGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC424588 ( XM_422428.6 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_422428.3 Gallus gallus E3 ubiquitin-protein ligase HECTD3-like (HECTD3L), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa39558
Clone ID Related Accession (Same CDS sequence)	XM_422428.5 , XM_422428.6
Accession Version	XM_422428.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase HECTD3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_422428.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCGG GCGAGGAGGC CACGCACCAG GTGCTGGGCC GGCTGCGGTT CCTGCTGCAG 
TGCAGCGAGT GCTTCCGCCG CGCCCGGCCG CTGCCCGCCG CGCTGTGCTA CGTGCCCCGC 
GAGGTGCAGT ACAAGATCTG CAAGGACCCC TCGGCCGCCG CCTCGTCCCG CAGCCTGCTC 
AGCGTCTGGG ACGGCCCCGG GCCGGCGCGG GGCTCCAAGC GGGCCACCAT CGAGGTGCGG 
AAGGGCGGCT GTCTCCGTGC TACGGGAGAG GAGTACCGCA ACAGCGCGGG GCTGTGGGTG 
AAGCTCCGCA AGGAGCACCT GGAGGAGCAC GGGGTCTGCC GTGAGCTGGA GGAGGGCTGG 
GTCCTGGCGC AGCGGTTCGG AGATGGGGGA GATAAGTTGG TGCCGGTGGA GAGGATCCAG 
CAGCAACAGC AGACCTTTGG AACGGATTAC AAACCTGCGG CCAGCTGGGA ACAAGTGGTG 
GACCTGATGT ACTCCATGCA GCTGGGAGAG AAGCCCGTGC CAGTCGAGCC GGATGAGGCT 
GTGGTGCAGA AGCTTCGGTC GATGCCCCCC AATTGGAGCT ACGAGCAGGA CATGGAGCTG 
GGGCGCTTCC TGTACGACGT CACTGAGAGG GATGAACACT GCAAGGACTG CGTTAAGGAC 
CACCTCAATG GCATAGAGGT CTCCTCACAA CTGGATGACT TCAAAGTATC CCACCTGACA 
GACAACCAGG GAGACACCTA CTGGGAGAGC AGCGGGTCCC CTGGGCAGCA TTGGGTGCGG 
CTCCACATGA AGAAAGGCAG CGTCATCAAG AAGCTGTGGC TGATGCTGAA CGCAAACATC 
AACTCCTACG TCCCCAGGAG GGTGGCTGTG TACGGAGGGC CAGCCAACAG GCTGCAGCAC 
CTGAGAACCG TCCTAATCAA CGAGAACAGC TATCAAGATG TGTGCATCCT ACGTGACATG 
AAGACCCACG TGCCGGTCCT GGAGATCCGC ATCTTGGAGT GCCGAGAACA AGGGTACAAC 
GTCCGGCTGC GAGGGATTAA GTTCAAGTCC TTCTGGGAGA GGGACCTGAA CCTCAACGCA 
GACATGTTCC AGATGGCCCA GCTGGTGCGC TACCCACTGC TGGAAGGGGT GGACACAGAT 
GTGCTGTACC GCAGGGCTGT AGTCATCCAG AGGTTCACCC AGCTCCTGGA CAGTGTCCTG 
GGGTACCTGA TCCCCATCTC TGATGAAAGT GACAGCACCT TCAGTGTGCT CAGGGGCATG 
AAGCCATTCC TGCAGCTGTG CAAGCTGAGC AAAACCCTCG TCACCCATTG CCTGCAGAGC 
TCAGAGAGCA GCCCACCTTG CATGCTGCCA AAGCTGCTCA TCAACCGGCA GCTGGCCCGT 
GAGCACCGGG CTCACCCTGA GCTGGACCCT AGTGGCCGGA ACACAGTCTT CACCCAGGTG 
TATGAAAACC TGAAGCCTTC CAAAACCAAC AACCATCTCC TAAACTACAG GTGGCCTCAG 
ACCCACAGCC AGTGGTGGGA GTGTGTCTTC ACCACGGAGG GCATCATCGA TAATGGCGGT 
GGTTTTCGGG ACTGCTTGAC AGACATCTCA GAAGAGCTGT GTCCCAGCTC GGGCGATGTC 
CCCGTGCCTC TGCCCTTCTT TGTGCGCACA TCCAACCAGG GTAACAGCAG CAGCGACACC 
AGGGACATGT ACGTCCCCAA CCCCTCCTGC AAGGACTTCC CTAAGTATGA GTGGATCGGA 
CAGCTCATGG GAGCAGCCCT GAGGAGCAAG GAGATTCTGG TCCTGGCTCT GCCCAGTCTG 
GTGTGGAAGC AGCTGTCAGG GGAGGAGGTC ATCTGGAGCA AGGACTTTGC TGCTGTGGAT 
GTGGAGCTGG TGAAGCTGCT GCAGATGCTG GAGGAAGTGG ACAGGGAGGC CTTCAACTTC 
ATGTTTGGCA AGGAGCTGAC CTACACAACG GTACGGAGCG ACCAGCGCGT GGTGGAGCTG 
ATCCCAAAGG GCAGCAGCAC TGTGGTGCGC TTTGAGGACC GCAAGGAGTT CATCCGTCTG 
GTGCAGAAGG CACGGCTGGA GGAGAGCAAG GAGCAGGTGG AGGCCATTCG TGCCGGGCTG 
CTCCGTGTGG TGCCTCAGGC TGTGCTGGAC CTGCTCACCT GGCAGCAGCT GGAGAAGAAG 
GTCTGTGGGA ACCCCGAGGT GACGGTGGAC GAGCTGAAGA AGTTTGTCAC CTTTGAGGAC 
TTTGACAGCT CCCACGTCCA GCAATTCTGG GATGCCCTCA ACAACTTCAC CAGCGAGGAC 
CTCAGCCGCT TCCTCAAGTT CGTCACCGGC CGCAGCCGCC TCCCTGTTCA GCTCACCATC 
TACCCAGACC GGTCCATCCC TGAGCGGTTG GACATGATGC CTGAGGCCTC CACGTGCTCC 
TGCACCTTGT TCTTGCCCAA ATACTCCTCG GTCAAGACGT GTGAGGAGCT GCTGCGCTAC 
GCCGTGTATA ACTGCATGTC CATCGACACC GACAAGGACA ACTGGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_422428.3
CDS	156..2666
Translation	MRAGEEATHQVLGRLRFLLQCSECFRRARPLPAALCYVPREVQYKICKDPSAAASSRSLLSVWDGPGPARGSKRATIEVRKGGCLRATGEEYRNSAGLWVKLRKEHLEEHGVCRELEEGWVLAQRFGDGGDKLVPVERIQQQQQTFGTDYKPAASWEQVVDLMYSMQLGEKPVPVEPDEAVVQKLRSMPPNWSYEQDMELGRFLYDVTERDEHCKDCVKDHLNGIEVSSQLDDFKVSHLTDNQGDTYWESSGSPGQHWVRLHMKKGSVIKKLWLMLNANINSYVPRRVAVYGGPANRLQHLRTVLINENSYQDVCILRDMKTHVPVLEIRILECREQGYNVRLRGIKFKSFWERDLNLNADMFQMAQLVRYPLLEGVDTDVLYRRAVVIQRFTQLLDSVLGYLIPISDESDSTFSVLRGMKPFLQLCKLSKTLVTHCLQSSESSPPCMLPKLLINRQLAREHRAHPELDPSGRNTVFTQVYENLKPSKTNNHLLNYRWPQTHSQWWECVFTTEGIIDNGGGFRDCLTDISEELCPSSGDVPVPLPFFVRTSNQGNSSSDTRDMYVPNPSCKDFPKYEWIGQLMGAALRSKEILVLALPSLVWKQLSGEEVIWSKDFAAVDVELVKLLQMLEEVDREAFNFMFGKELTYTTVRSDQRVVELIPKGSSTVVRFEDRKEFIRLVQKARLEESKEQVEAIRAGLLRVVPQAVLDLLTWQQLEKKVCGNPEVTVDELKKFVTFEDFDSSHVQQFWDALNNFTSEDLSRFLKFVTGRSRLPVQLTIYPDRSIPERLDMMPEASTCSCTLFLPKYSSVKTCEELLRYAVYNCMSIDTDKDNWD

Target ORF information:

RefSeq Version	XM_422428.6
Organism	Gallus gallus(chicken)
Definition	Gallus gallus E3 ubiquitin-protein ligase HECTD3-like (HECTD3L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_422428.6

ORF Insert Sequence:

ATGCGGGCGG GCGAGGAGGC CACGCACCAG GTGCTGGGCC GGCTGCGGTT CCTGCTGCAG 
TGCAGCGAGT GCTTCCGCCG CGCCCGGCCG CTGCCCGCCG CGCTGTGCTA CGTGCCCCGC 
GAGGTGCAGT ACAAGATCTG CAAGGACCCC TCGGCCGCCG CCTCGTCCCG CAGCCTGCTC 
AGCGTCTGGG ACGGCCCCGG GCCGGCGCGG GGCTCCAAGC GGGCCACCAT CGAGGTGCGG 
AAGGGCGGCT GTCTCCGTGC TACGGGAGAG GAGTACCGCA ACAGCGCGGG GCTGTGGGTG 
AAGCTCCGCA AGGAGCACCT GGAGGAGCAC GGGGTCTGCC GTGAGCTGGA GGAGGGCTGG 
GTCCTGGCGC AGCGGTTCGG AGATGGGGGA GATAAGTTGG TGCCGGTGGA GAGGATCCAG 
CAGCAACAGC AGACCTTTGG AACGGATTAC AAACCTGCGG CCAGCTGGGA ACAAGTGGTG 
GACCTGATGT ACTCCATGCA GCTGGGAGAG AAGCCCGTGC CAGTCGAGCC GGATGAGGCT 
GTGGTGCAGA AGCTTCGGTC GATGCCCCCC AATTGGAGCT ACGAGCAGGA CATGGAGCTG 
GGGCGCTTCC TGTACGACGT CACTGAGAGG GATGAACACT GCAAGGACTG CGTTAAGGAC 
CACCTCAATG GCATAGAGGT CTCCTCACAA CTGGATGACT TCAAAGTATC CCACCTGACA 
GACAACCAGG GAGACACCTA CTGGGAGAGC AGCGGGTCCC CTGGGCAGCA TTGGGTGCGG 
CTCCACATGA AGAAAGGCAG CGTCATCAAG AAGCTGTGGC TGATGCTGAA CGCAAACATC 
AACTCCTACG TCCCCAGGAG GGTGGCTGTG TACGGAGGGC CAGCCAACAG GCTGCAGCAC 
CTGAGAACCG TCCTAATCAA CGAGAACAGC TATCAAGATG TGTGCATCCT ACGTGACATG 
AAGACCCACG TGCCGGTCCT GGAGATCCGC ATCTTGGAGT GCCGAGAACA AGGGTACAAC 
GTCCGGCTGC GAGGGATTAA GTTCAAGTCC TTCTGGGAGA GGGACCTGAA CCTCAACGCA 
GACATGTTCC AGATGGCCCA GCTGGTGCGC TACCCACTGC TGGAAGGGGT GGACACAGAT 
GTGCTGTACC GCAGGGCTGT AGTCATCCAG AGGTTCACCC AGCTCCTGGA CAGTGTCCTG 
GGGTACCTGA TCCCCATCTC TGATGAAAGT GACAGCACCT TCAGTGTGCT CAGGGGCATG 
AAGCCATTCC TGCAGCTGTG CAAGCTGAGC AAAACCCTCG TCACCCATTG CCTGCAGAGC 
TCAGAGAGCA GCCCACCTTG CATGCTGCCA AAGCTGCTCA TCAACCGGCA GCTGGCCCGT 
GAGCACCGGG CTCACCCTGA GCTGGACCCT AGTGGCCGGA ACACAGTCTT CACCCAGGTG 
TATGAAAACC TGAAGCCTTC CAAAACCAAC AACCATCTCC TAAACTACAG GTGGCCTCAG 
ACCCACAGCC AGTGGTGGGA GTGTGTCTTC ACCACGGAGG GCATCATCGA TAATGGCGGT 
GGTTTTCGGG ACTGCTTGAC AGACATCTCA GAAGAGCTGT GTCCCAGCTC GGGCGATGTC 
CCCGTGCCTC TGCCCTTCTT TGTGCGCACA TCCAACCAGG GTAACAGCAG CAGCGACACC 
AGGGACATGT ACGTCCCCAA CCCCTCCTGC AAGGACTTCC CTAAGTATGA GTGGATCGGA 
CAGCTCATGG GAGCAGCCCT GAGGAGCAAG GAGATTCTGG TCCTGGCTCT GCCCAGTCTG 
GTGTGGAAGC AGCTGTCAGG GGAGGAGGTC ATCTGGAGCA AGGACTTTGC TGCTGTGGAT 
GTGGAGCTGG TGAAGCTGCT GCAGATGCTG GAGGAAGTGG ACAGGGAGGC CTTCAACTTC 
ATGTTTGGCA AGGAGCTGAC CTACACAACG GTACGGAGCG ACCAGCGCGT GGTGGAGCTG 
ATCCCAAAGG GCAGCAGCAC TGTGGTGCGC TTTGAGGACC GCAAGGAGTT CATCCGTCTG 
GTGCAGAAGG CACGGCTGGA GGAGAGCAAG GAGCAGGTGG AGGCCATTCG TGCCGGGCTG 
CTCCGTGTGG TGCCTCAGGC TGTGCTGGAC CTGCTCACCT GGCAGCAGCT GGAGAAGAAG 
GTCTGTGGGA ACCCCGAGGT GACGGTGGAC GAGCTGAAGA AGTTTGTCAC CTTTGAGGAC 
TTTGACAGCT CCCACGTCCA GCAATTCTGG GATGCCCTCA ACAACTTCAC CAGCGAGGAC 
CTCAGCCGCT TCCTCAAGTT CGTCACCGGC CGCAGCCGCC TCCCTGTTCA GCTCACCATC 
TACCCAGACC GGTCCATCCC TGAGCGGTTG GACATGATGC CTGAGGCCTC CACGTGCTCC 
TGCACCTTGT TCTTGCCCAA ATACTCCTCG GTCAAGACGT GTGAGGAGCT GCTGCGCTAC 
GCCGTGTATA ACTGCATGTC CATCGACACC GACAAGGACA ACTGGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC424588 ( XM_015291285.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015146771.1 Gallus gallus E3 ubiquitin-protein ligase HECTD3-like (HECTD3L), transcript variant X4, mRNA.

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CloneID	OGa39559
Clone ID Related Accession (Same CDS sequence)	XM_015291285.2 , XM_015291285.1
Accession Version	XM_015291285.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase HECTD3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291285.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCGG GCGAGGAGGC CACGCACCAG GTGCTGGGCC GGCTGCGGTT CCTGCTGCAG 
TGCAGCGAGT GCTTCCGCCG CGCCCGGCCG CTGCCCGCCG CGCTGTGCTA CGTGCCCCGC 
GAGGTGCAGT ACAAGATCTG CAAGGACCCC TCGGCCGCCG CCTCGTCCCG CAGCCTGCTC 
AGCGTCTGGG ACGGCCCCGG GCCGGCGCGG GGCTCCAAGC GGGCCACCAT CGAGGTGCGG 
AAGGGCGGCT GTCTCCGTGC TACGGGAGAG GAGTACCGCA ACAGCGCGGG GCTGTGGGTG 
AAGCTCCGCA AGGAGCACCT GGAGGAGCAC GGGGTCTGCC GTGAGCTGGA GGAGGGCTGG 
GTCCTGGCGC AGCGGTTCGG AGATGGGGGA GATAAGTTGG TGCCGGTGGA GAGGATCCAG 
CAGCAACAGC AGACCTTTGG AACGGATTAC AAACCTGCGG CCAGCTGGGA ACAAGTGGTG 
GACCTGATGT ACTCCATGCA GCTGGGAGAG AAGCCCGTGC CAGTCGAGCC GGATGAGGCT 
GTGGTGCAGA AGCTTCGGTC GATGCCCCCC AATTGGAGCT ACGAGCAGGA CATGGAGCTG 
GGGCGCTTCC TGTACGACGT CACTGAGAGG GATGAACACT GCAAGGACTG CGTTAAGGAC 
CACCTCAATG GCATAGAGGT CTCCTCACAA CTGGATGACT TCAAAGTATC CCACCTGACA 
GACAACCAGG GAGACACCTA CTGGGAGAGC AGCGGGTCCC CTGGGCAGCA TTGGGTGCGG 
CTCCACATGA AGAAAGGCAG CGTCATCAAG AAGCTGTGGC TGATGCTGAA CGCAAACATC 
AACTCCTACG TCCCCAGGAG GGTGGCTGTG TACGGAGGGC CAGCCAACAG GCTGCAGCAC 
CTGAGAACCG TCCTAATCAA CGACCGCTCC TCTCTGCTCC ATCCCCTCCT GCTGCAGCCC 
TGGAACAGCT ATCAAGATGT GTGCATCCTA CGTGACATGA AGACCCACGT GCCGGTCCTG 
GAGATCCGCA TCTTGGAGTG CCGAGAACAA GGGTACAACG TCCGGCTGCG AGGGATTAAG 
TTCAAGTCCT TCTGGGAGAG GGACCTGAAC CTCAACGCAG ACATGTTCCA GATGGCCCAG 
CTGGTGCGCT ACCCACTGCT GGAAGGGGTG GACACAGATG TGCTGTACCG CAGGGCTGTA 
GTCATCCAGA GGTTCACCCA GCTCCTGGAC AGTGTCCTGG GGTACCTGAT CCCCATCTCT 
GATGAAAGTG ACAGCACCTT CAGTGTGCTC AGGGGCATGA AGCCATTCCT GCAGCTGTGC 
AAGCTGAGCA AAACCCTCGT CACCCATTGC CTGCAGAGCT CAGAGAGCAG CCCACCTTGC 
ATGCTGCCAA AGCTGCTCAT CAACCGGCAG CTGGCCCGTG AGCACCGGGC TCACCCTGAG 
CTGGACCCTA GTGGCCGGAA CACAGTCTTC ACCCAGGTGT ATGAAAACCT GAAGCCTTCC 
AAAACCAACA ACCATCTCCT AAACTACAGG TGGCCTCAGA CCCACAGCCA GTGGTGGGAG 
TGTGTCTTCA CCACGGAGGG CATCATCGAT AATGGCGGTG GTTTTCGGGA CTGCTTGACA 
GACATCTCAG AAGAGCTGTG TCCCAGCTCG GGCGATGTCC CCGTGCCTCT GCCCTTCTTT 
GTGCGCACAT CCAACCAGGG TAACAGCAGC AGCGACACCA GGGACATGTA CGTCCCCAAC 
CCCTCCTGCA AGGACTTCCC TAAGTATGAG TGGATCGGAC AGCTCATGGG AGCAGCCCTG 
AGGAGCAAGG AGATTCTGGT CCTGGCTCTG CCCAGTCTGG TGTGGAAGCA GCTGTCAGGG 
GAGGAGGTCA TCTGGAGCAA GGACTTTGCT GCTGTGGATG TGGAGCTGGT GAAGCTGCTG 
CAGATGCTGG AGGAAGTGGA CAGGGAGGCC TTCAACTTCA TGTTTGGCAA GGAGCTGACC 
TACACAACGG TACGGAGCGA CCAGCGCGTG GTGGAGCTGA TCCCAAAGGG CAGCAGCACT 
GTGGTGCGCT TTGAGGACCG CAAGGAGTTC ATCCGTCTGG TGCAGAAGGC ACGGCTGGAG 
GAGAGCAAGG AGCAGTGGGT TAGGGGCCTG CCGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015146771.1
CDS	167..2305
Translation	MRAGEEATHQVLGRLRFLLQCSECFRRARPLPAALCYVPREVQYKICKDPSAAASSRSLLSVWDGPGPARGSKRATIEVRKGGCLRATGEEYRNSAGLWVKLRKEHLEEHGVCRELEEGWVLAQRFGDGGDKLVPVERIQQQQQTFGTDYKPAASWEQVVDLMYSMQLGEKPVPVEPDEAVVQKLRSMPPNWSYEQDMELGRFLYDVTERDEHCKDCVKDHLNGIEVSSQLDDFKVSHLTDNQGDTYWESSGSPGQHWVRLHMKKGSVIKKLWLMLNANINSYVPRRVAVYGGPANRLQHLRTVLINDRSSLLHPLLLQPWNSYQDVCILRDMKTHVPVLEIRILECREQGYNVRLRGIKFKSFWERDLNLNADMFQMAQLVRYPLLEGVDTDVLYRRAVVIQRFTQLLDSVLGYLIPISDESDSTFSVLRGMKPFLQLCKLSKTLVTHCLQSSESSPPCMLPKLLINRQLAREHRAHPELDPSGRNTVFTQVYENLKPSKTNNHLLNYRWPQTHSQWWECVFTTEGIIDNGGGFRDCLTDISEELCPSSGDVPVPLPFFVRTSNQGNSSSDTRDMYVPNPSCKDFPKYEWIGQLMGAALRSKEILVLALPSLVWKQLSGEEVIWSKDFAAVDVELVKLLQMLEEVDREAFNFMFGKELTYTTVRSDQRVVELIPKGSSTVVRFEDRKEFIRLVQKARLEESKEQWVRGLPR

Target ORF information:

RefSeq Version	XM_015291285.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus E3 ubiquitin-protein ligase HECTD3-like (HECTD3L), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291285.2

ORF Insert Sequence:

ATGCGGGCGG GCGAGGAGGC CACGCACCAG GTGCTGGGCC GGCTGCGGTT CCTGCTGCAG 
TGCAGCGAGT GCTTCCGCCG CGCCCGGCCG CTGCCCGCCG CGCTGTGCTA CGTGCCCCGC 
GAGGTGCAGT ACAAGATCTG CAAGGACCCC TCGGCCGCCG CCTCGTCCCG CAGCCTGCTC 
AGCGTCTGGG ACGGCCCCGG GCCGGCGCGG GGCTCCAAGC GGGCCACCAT CGAGGTGCGG 
AAGGGCGGCT GTCTCCGTGC TACGGGAGAG GAGTACCGCA ACAGCGCGGG GCTGTGGGTG 
AAGCTCCGCA AGGAGCACCT GGAGGAGCAC GGGGTCTGCC GTGAGCTGGA GGAGGGCTGG 
GTCCTGGCGC AGCGGTTCGG AGATGGGGGA GATAAGTTGG TGCCGGTGGA GAGGATCCAG 
CAGCAACAGC AGACCTTTGG AACGGATTAC AAACCTGCGG CCAGCTGGGA ACAAGTGGTG 
GACCTGATGT ACTCCATGCA GCTGGGAGAG AAGCCCGTGC CAGTCGAGCC GGATGAGGCT 
GTGGTGCAGA AGCTTCGGTC GATGCCCCCC AATTGGAGCT ACGAGCAGGA CATGGAGCTG 
GGGCGCTTCC TGTACGACGT CACTGAGAGG GATGAACACT GCAAGGACTG CGTTAAGGAC 
CACCTCAATG GCATAGAGGT CTCCTCACAA CTGGATGACT TCAAAGTATC CCACCTGACA 
GACAACCAGG GAGACACCTA CTGGGAGAGC AGCGGGTCCC CTGGGCAGCA TTGGGTGCGG 
CTCCACATGA AGAAAGGCAG CGTCATCAAG AAGCTGTGGC TGATGCTGAA CGCAAACATC 
AACTCCTACG TCCCCAGGAG GGTGGCTGTG TACGGAGGGC CAGCCAACAG GCTGCAGCAC 
CTGAGAACCG TCCTAATCAA CGACCGCTCC TCTCTGCTCC ATCCCCTCCT GCTGCAGCCC 
TGGAACAGCT ATCAAGATGT GTGCATCCTA CGTGACATGA AGACCCACGT GCCGGTCCTG 
GAGATCCGCA TCTTGGAGTG CCGAGAACAA GGGTACAACG TCCGGCTGCG AGGGATTAAG 
TTCAAGTCCT TCTGGGAGAG GGACCTGAAC CTCAACGCAG ACATGTTCCA GATGGCCCAG 
CTGGTGCGCT ACCCACTGCT GGAAGGGGTG GACACAGATG TGCTGTACCG CAGGGCTGTA 
GTCATCCAGA GGTTCACCCA GCTCCTGGAC AGTGTCCTGG GGTACCTGAT CCCCATCTCT 
GATGAAAGTG ACAGCACCTT CAGTGTGCTC AGGGGCATGA AGCCATTCCT GCAGCTGTGC 
AAGCTGAGCA AAACCCTCGT CACCCATTGC CTGCAGAGCT CAGAGAGCAG CCCACCTTGC 
ATGCTGCCAA AGCTGCTCAT CAACCGGCAG CTGGCCCGTG AGCACCGGGC TCACCCTGAG 
CTGGACCCTA GTGGCCGGAA CACAGTCTTC ACCCAGGTGT ATGAAAACCT GAAGCCTTCC 
AAAACCAACA ACCATCTCCT AAACTACAGG TGGCCTCAGA CCCACAGCCA GTGGTGGGAG 
TGTGTCTTCA CCACGGAGGG CATCATCGAT AATGGCGGTG GTTTTCGGGA CTGCTTGACA 
GACATCTCAG AAGAGCTGTG TCCCAGCTCG GGCGATGTCC CCGTGCCTCT GCCCTTCTTT 
GTGCGCACAT CCAACCAGGG TAACAGCAGC AGCGACACCA GGGACATGTA CGTCCCCAAC 
CCCTCCTGCA AGGACTTCCC TAAGTATGAG TGGATCGGAC AGCTCATGGG AGCAGCCCTG 
AGGAGCAAGG AGATTCTGGT CCTGGCTCTG CCCAGTCTGG TGTGGAAGCA GCTGTCAGGG 
GAGGAGGTCA TCTGGAGCAA GGACTTTGCT GCTGTGGATG TGGAGCTGGT GAAGCTGCTG 
CAGATGCTGG AGGAAGTGGA CAGGGAGGCC TTCAACTTCA TGTTTGGCAA GGAGCTGACC 
TACACAACGG TACGGAGCGA CCAGCGCGTG GTGGAGCTGA TCCCAAAGGG CAGCAGCACT 
GTGGTGCGCT TTGAGGACCG CAAGGAGTTC ATCCGTCTGG TGCAGAAGGC ACGGCTGGAG 
GAGAGCAAGG AGCAGTGGGT TAGGGGCCTG CCGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC424588 ( XM_015291285.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015146771.1 Gallus gallus E3 ubiquitin-protein ligase HECTD3-like (LOC424588), transcript variant X3, mRNA.

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CloneID	OGa39559
Clone ID Related Accession (Same CDS sequence)	XM_015291285.2 , XM_015291285.1
Accession Version	XM_015291285.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase HECTD3-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCGG GCGAGGAGGC CACGCACCAG GTGCTGGGCC GGCTGCGGTT CCTGCTGCAG 
TGCAGCGAGT GCTTCCGCCG CGCCCGGCCG CTGCCCGCCG CGCTGTGCTA CGTGCCCCGC 
GAGGTGCAGT ACAAGATCTG CAAGGACCCC TCGGCCGCCG CCTCGTCCCG CAGCCTGCTC 
AGCGTCTGGG ACGGCCCCGG GCCGGCGCGG GGCTCCAAGC GGGCCACCAT CGAGGTGCGG 
AAGGGCGGCT GTCTCCGTGC TACGGGAGAG GAGTACCGCA ACAGCGCGGG GCTGTGGGTG 
AAGCTCCGCA AGGAGCACCT GGAGGAGCAC GGGGTCTGCC GTGAGCTGGA GGAGGGCTGG 
GTCCTGGCGC AGCGGTTCGG AGATGGGGGA GATAAGTTGG TGCCGGTGGA GAGGATCCAG 
CAGCAACAGC AGACCTTTGG AACGGATTAC AAACCTGCGG CCAGCTGGGA ACAAGTGGTG 
GACCTGATGT ACTCCATGCA GCTGGGAGAG AAGCCCGTGC CAGTCGAGCC GGATGAGGCT 
GTGGTGCAGA AGCTTCGGTC GATGCCCCCC AATTGGAGCT ACGAGCAGGA CATGGAGCTG 
GGGCGCTTCC TGTACGACGT CACTGAGAGG GATGAACACT GCAAGGACTG CGTTAAGGAC 
CACCTCAATG GCATAGAGGT CTCCTCACAA CTGGATGACT TCAAAGTATC CCACCTGACA 
GACAACCAGG GAGACACCTA CTGGGAGAGC AGCGGGTCCC CTGGGCAGCA TTGGGTGCGG 
CTCCACATGA AGAAAGGCAG CGTCATCAAG AAGCTGTGGC TGATGCTGAA CGCAAACATC 
AACTCCTACG TCCCCAGGAG GGTGGCTGTG TACGGAGGGC CAGCCAACAG GCTGCAGCAC 
CTGAGAACCG TCCTAATCAA CGACCGCTCC TCTCTGCTCC ATCCCCTCCT GCTGCAGCCC 
TGGAACAGCT ATCAAGATGT GTGCATCCTA CGTGACATGA AGACCCACGT GCCGGTCCTG 
GAGATCCGCA TCTTGGAGTG CCGAGAACAA GGGTACAACG TCCGGCTGCG AGGGATTAAG 
TTCAAGTCCT TCTGGGAGAG GGACCTGAAC CTCAACGCAG ACATGTTCCA GATGGCCCAG 
CTGGTGCGCT ACCCACTGCT GGAAGGGGTG GACACAGATG TGCTGTACCG CAGGGCTGTA 
GTCATCCAGA GGTTCACCCA GCTCCTGGAC AGTGTCCTGG GGTACCTGAT CCCCATCTCT 
GATGAAAGTG ACAGCACCTT CAGTGTGCTC AGGGGCATGA AGCCATTCCT GCAGCTGTGC 
AAGCTGAGCA AAACCCTCGT CACCCATTGC CTGCAGAGCT CAGAGAGCAG CCCACCTTGC 
ATGCTGCCAA AGCTGCTCAT CAACCGGCAG CTGGCCCGTG AGCACCGGGC TCACCCTGAG 
CTGGACCCTA GTGGCCGGAA CACAGTCTTC ACCCAGGTGT ATGAAAACCT GAAGCCTTCC 
AAAACCAACA ACCATCTCCT AAACTACAGG TGGCCTCAGA CCCACAGCCA GTGGTGGGAG 
TGTGTCTTCA CCACGGAGGG CATCATCGAT AATGGCGGTG GTTTTCGGGA CTGCTTGACA 
GACATCTCAG AAGAGCTGTG TCCCAGCTCG GGCGATGTCC CCGTGCCTCT GCCCTTCTTT 
GTGCGCACAT CCAACCAGGG TAACAGCAGC AGCGACACCA GGGACATGTA CGTCCCCAAC 
CCCTCCTGCA AGGACTTCCC TAAGTATGAG TGGATCGGAC AGCTCATGGG AGCAGCCCTG 
AGGAGCAAGG AGATTCTGGT CCTGGCTCTG CCCAGTCTGG TGTGGAAGCA GCTGTCAGGG 
GAGGAGGTCA TCTGGAGCAA GGACTTTGCT GCTGTGGATG TGGAGCTGGT GAAGCTGCTG 
CAGATGCTGG AGGAAGTGGA CAGGGAGGCC TTCAACTTCA TGTTTGGCAA GGAGCTGACC 
TACACAACGG TACGGAGCGA CCAGCGCGTG GTGGAGCTGA TCCCAAAGGG CAGCAGCACT 
GTGGTGCGCT TTGAGGACCG CAAGGAGTTC ATCCGTCTGG TGCAGAAGGC ACGGCTGGAG 
GAGAGCAAGG AGCAGTGGGT TAGGGGCCTG CCGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015146771.1
CDS	85..2223
Translation	MRAGEEATHQVLGRLRFLLQCSECFRRARPLPAALCYVPREVQYKICKDPSAAASSRSLLSVWDGPGPARGSKRATIEVRKGGCLRATGEEYRNSAGLWVKLRKEHLEEHGVCRELEEGWVLAQRFGDGGDKLVPVERIQQQQQTFGTDYKPAASWEQVVDLMYSMQLGEKPVPVEPDEAVVQKLRSMPPNWSYEQDMELGRFLYDVTERDEHCKDCVKDHLNGIEVSSQLDDFKVSHLTDNQGDTYWESSGSPGQHWVRLHMKKGSVIKKLWLMLNANINSYVPRRVAVYGGPANRLQHLRTVLINDRSSLLHPLLLQPWNSYQDVCILRDMKTHVPVLEIRILECREQGYNVRLRGIKFKSFWERDLNLNADMFQMAQLVRYPLLEGVDTDVLYRRAVVIQRFTQLLDSVLGYLIPISDESDSTFSVLRGMKPFLQLCKLSKTLVTHCLQSSESSPPCMLPKLLINRQLAREHRAHPELDPSGRNTVFTQVYENLKPSKTNNHLLNYRWPQTHSQWWECVFTTEGIIDNGGGFRDCLTDISEELCPSSGDVPVPLPFFVRTSNQGNSSSDTRDMYVPNPSCKDFPKYEWIGQLMGAALRSKEILVLALPSLVWKQLSGEEVIWSKDFAAVDVELVKLLQMLEEVDREAFNFMFGKELTYTTVRSDQRVVELIPKGSSTVVRFEDRKEFIRLVQKARLEESKEQWVRGLPR

Target ORF information:

RefSeq Version	XM_015291285.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus E3 ubiquitin-protein ligase HECTD3-like (LOC424588), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291285.1

ORF Insert Sequence:

ATGCGGGCGG GCGAGGAGGC CACGCACCAG GTGCTGGGCC GGCTGCGGTT CCTGCTGCAG 
TGCAGCGAGT GCTTCCGCCG CGCCCGGCCG CTGCCCGCCG CGCTGTGCTA CGTGCCCCGC 
GAGGTGCAGT ACAAGATCTG CAAGGACCCC TCGGCCGCCG CCTCGTCCCG CAGCCTGCTC 
AGCGTCTGGG ACGGCCCCGG GCCGGCGCGG GGCTCCAAGC GGGCCACCAT CGAGGTGCGG 
AAGGGCGGCT GTCTCCGTGC TACGGGAGAG GAGTACCGCA ACAGCGCGGG GCTGTGGGTG 
AAGCTCCGCA AGGAGCACCT GGAGGAGCAC GGGGTCTGCC GTGAGCTGGA GGAGGGCTGG 
GTCCTGGCGC AGCGGTTCGG AGATGGGGGA GATAAGTTGG TGCCGGTGGA GAGGATCCAG 
CAGCAACAGC AGACCTTTGG AACGGATTAC AAACCTGCGG CCAGCTGGGA ACAAGTGGTG 
GACCTGATGT ACTCCATGCA GCTGGGAGAG AAGCCCGTGC CAGTCGAGCC GGATGAGGCT 
GTGGTGCAGA AGCTTCGGTC GATGCCCCCC AATTGGAGCT ACGAGCAGGA CATGGAGCTG 
GGGCGCTTCC TGTACGACGT CACTGAGAGG GATGAACACT GCAAGGACTG CGTTAAGGAC 
CACCTCAATG GCATAGAGGT CTCCTCACAA CTGGATGACT TCAAAGTATC CCACCTGACA 
GACAACCAGG GAGACACCTA CTGGGAGAGC AGCGGGTCCC CTGGGCAGCA TTGGGTGCGG 
CTCCACATGA AGAAAGGCAG CGTCATCAAG AAGCTGTGGC TGATGCTGAA CGCAAACATC 
AACTCCTACG TCCCCAGGAG GGTGGCTGTG TACGGAGGGC CAGCCAACAG GCTGCAGCAC 
CTGAGAACCG TCCTAATCAA CGACCGCTCC TCTCTGCTCC ATCCCCTCCT GCTGCAGCCC 
TGGAACAGCT ATCAAGATGT GTGCATCCTA CGTGACATGA AGACCCACGT GCCGGTCCTG 
GAGATCCGCA TCTTGGAGTG CCGAGAACAA GGGTACAACG TCCGGCTGCG AGGGATTAAG 
TTCAAGTCCT TCTGGGAGAG GGACCTGAAC CTCAACGCAG ACATGTTCCA GATGGCCCAG 
CTGGTGCGCT ACCCACTGCT GGAAGGGGTG GACACAGATG TGCTGTACCG CAGGGCTGTA 
GTCATCCAGA GGTTCACCCA GCTCCTGGAC AGTGTCCTGG GGTACCTGAT CCCCATCTCT 
GATGAAAGTG ACAGCACCTT CAGTGTGCTC AGGGGCATGA AGCCATTCCT GCAGCTGTGC 
AAGCTGAGCA AAACCCTCGT CACCCATTGC CTGCAGAGCT CAGAGAGCAG CCCACCTTGC 
ATGCTGCCAA AGCTGCTCAT CAACCGGCAG CTGGCCCGTG AGCACCGGGC TCACCCTGAG 
CTGGACCCTA GTGGCCGGAA CACAGTCTTC ACCCAGGTGT ATGAAAACCT GAAGCCTTCC 
AAAACCAACA ACCATCTCCT AAACTACAGG TGGCCTCAGA CCCACAGCCA GTGGTGGGAG 
TGTGTCTTCA CCACGGAGGG CATCATCGAT AATGGCGGTG GTTTTCGGGA CTGCTTGACA 
GACATCTCAG AAGAGCTGTG TCCCAGCTCG GGCGATGTCC CCGTGCCTCT GCCCTTCTTT 
GTGCGCACAT CCAACCAGGG TAACAGCAGC AGCGACACCA GGGACATGTA CGTCCCCAAC 
CCCTCCTGCA AGGACTTCCC TAAGTATGAG TGGATCGGAC AGCTCATGGG AGCAGCCCTG 
AGGAGCAAGG AGATTCTGGT CCTGGCTCTG CCCAGTCTGG TGTGGAAGCA GCTGTCAGGG 
GAGGAGGTCA TCTGGAGCAA GGACTTTGCT GCTGTGGATG TGGAGCTGGT GAAGCTGCTG 
CAGATGCTGG AGGAAGTGGA CAGGGAGGCC TTCAACTTCA TGTTTGGCAA GGAGCTGACC 
TACACAACGG TACGGAGCGA CCAGCGCGTG GTGGAGCTGA TCCCAAAGGG CAGCAGCACT 
GTGGTGCGCT TTGAGGACCG CAAGGAGTTC ATCCGTCTGG TGCAGAAGGC ACGGCTGGAG 
GAGAGCAAGG AGCAGTGGGT TAGGGGCCTG CCGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC424588 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

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