TCOF1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	TCOF1
Entrez Gene ID	416276
Full Name	treacle ribosome biogenesis factor 1
General protein information	Preferred Names treacle ribosome biogenesis factor 1 Names treacle protein Treacher Collins-Franceschetti syndrome 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015293789.1	XP_015149275.1	treacle protein isoform X2
XM_015293786.1	XP_015149272.1	treacle protein isoform X1
XM_015293790.1	XP_015149276.1	treacle protein isoform X3
XM_015293789.2	XP_015149275.1	treacle protein isoform X2
NM_001012785.2	NP_001012803.2	Treacher Collins-Franceschetti syndrome 1
XM_015293787.1	XP_015149273.1	treacle protein isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Caldwell RB, Kierzek AM, Arakawa H, Bezzubov Y, Zaim J, Fiedler P, Kutter S, Blagodatski A, Kostovska D, Koter M, Plachy J, Carninci P, Hayashizaki Y, Buerstedde JM
Genome biology6(1)R6(2005)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TCOF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TCOF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa00906	NM_001012785.2 Latest version!	Gallus gallus Treacher Collins-Franceschetti syndrome 1 (TCOF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OGa41927	XM_015293786.1 Latest version!	Gallus gallus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OGa41927	XM_015293787.1 Latest version!	Gallus gallus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OGa41928	XM_015293789.2 Latest version!	Gallus gallus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OGa41929	XM_015293790.1 Latest version!	Gallus gallus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
View Old Accession Versions >>
OGa41928	XM_015293789.1	Gallus gallus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TCOF1 ( NM_001012785.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001012803.2 Gallus gallus Treacher Collins-Franceschetti syndrome 1 (TCOF1), mRNA.

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CloneID	OGa00906
Clone ID Related Accession (Same CDS sequence)	NM_001012785.2
Accession Version	NM_001012785.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2012-05-04
Organism	Gallus gallus(chicken)
Product	Treacher Collins-Franceschetti syndrome 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN02028629.1. On Oct 17, 2007 this sequence version replaced NM_001012785.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGGCGGCGG AGGGCCGCGG GCGACGGGAG CTGCTGGCCC TCATCCACCA GCACCTCCTG 
CGCGCCGGCT ACGCGCGAGC CGCCCGCGAG CTACAGGCGC AGCTCGGACA GAAGCTGCTC 
CCGTCCCTGG CGACGTCGCT GGAGGAGATC TTCACGCACT GGGAGAAAAC TCCTTCGAAA 
GCCAGGAGAA GAAAGCTGAG TGATGAGGAG GCAGCCATCC CAGAAAAGAT CAGAGTTCCT 
GATCCTGTGA GCAGCTCTGA AAGCTCAGAG AAAGAAGAGG ATGAGAAAGA GAAGACGAAA 
ACTGGAAATG CAACCAGCAT TACCCCGGTC ACGAACTCAG CAGTGAATGT AGAAAGCAGT 
GAAGATGATG ACTCCTCATC AGAAGAAGAA ATGCCAAGTG GAAAAGGTGC AATTACGGTG 
ACACCAGCTG TAGCGAGTGG CAAAGCTGCC AACTCCTTGC ATTCTCCTGT TAAACCCACT 
GCTTCGGGAA GTAAAGCAGC GGTTCCCTGT GCTGCAAACA GAACTGTGCT CTCAAATAAG 
CAGCAGCCCA GTGCATCAGT TGTGACCCCA GCCAAAGCTG GGCAGAAACT GCCTAGTCCT 
GGGAAACCTG GACGAGATGC AGCAGCCGTG AGCCAAGCAG GACAAAGCAA AGCTCCAGTA 
ATGACCAAAG CACCGGAAAG CTCCAGCAGC AGCAGCTCGT CAGAGAGTGA GGAGGAAAAG 
GAGACACCGA CTGTGAAGGC CCCAGCCCCC AAAGTGGAGC TGAAACAGGC GGCTGGGGAA 
TCTGAGAGCA GCAGTGAGGA ATCAAGTGAT GAGACATCGT CTGAGGAGGA GCTTGCGACC 
GCAGCAGTCC AGGCAAAACC AGCTGTTAAA GCAGTGCAGG TTAATTCAAC ACCTGTGAAA 
AGTGCACCTA CTACTTCAGT GTCCACCAAG AAGAGCACAG TGAAAAAAAG AGCAGTGACA 
CTGAACCAGA CCAAGCATGG CTCAGTAACG GTTCCAGTGG CTGCAAAGCC TGATGACACC 
TCAGAGAGCA GCGACTCATC AGACAGTGAA AATGAGGAGC CTCCATCAGT AACCCAGTCA 
AAGCCATCTT TAAAAACCTC CCAGGCCATC CTATCCCCAG TGAAAACCAC ACCAGCAGCC 
TCAATCTCCA GCAAAGCAGC TCCAGCAAAA ACACTTCCAC TGTCCCAAGG GAAGCCAACG 
CCGAAACCGG CGGTGCTAAA GCAGGCTAAA GCCACACCAG GAAGGACTGC TGCTCCTGCA 
AAGCCAGCTG AAAGTGCAAA CCCAGCGGAG AGCACTGACT CTTCAGGCAG TGAAGAGGAT 
CTGCCTGTGC CTGTGAGCCA GAAGAGGCTA CCAGAACAGC CTGGGCCCGT TCCCAGCCTG 
AACCAGGCTG CTAAAGCCAT GACTCCTGAA AAGGCAGTGG GAAGCACCTT GAAGAATGAG 
ACCTCCGAAA GTGGAAGCAG TGACTCGTCT GACAGTGAGG AGGAGACTCC AGTGTCACAG 
ACACAGCCTG CACTGCCTGC TGCGAAAAGC AATGCTGTGC CCCAGCCAAC AAGCGTGAAG 
AAAGTAGCGG CTCCCACACC AGCCCCAGCC CCTCCTCTGC ATATGGACAG CAGTGATGAC 
TCCAGTGAGG AGTCGGATTT TGATGAGGAA ATAGTCCCCC CCACACAGGC TGTTCCTCCT 
CCTTGCCCAA AGCAGACAGC TCCCATGGGG AAGGCTCCAG CCAGCCCTGC TGCCCCGCAT 
GCTGCTTCCA TCCTGAGAAG CCCCACTGCC AAGTCGAAGA AGCCAGGCCA GCAGCCAGCA 
CAGGATGCCA GGCTGAGCCA GACTGCACCG CTCACCCAAG CAGAGGAGAG CTCGGACAGC 
AGCAGTTCCT CTGACAGCGA CGAGGAGACA TCCAAGCAGC CTCCCAAACC TGGGATGCTG 
CAGAAACCAG GAGGGACTGA GGCAAATGAC AGCTCCTCAT CGGATTCCAG TGAGGACGAA 
GAGGCAGTAT CACAGTCCCT CCTCACAGGT TATATGAGCC TCTCCAAGAG CCCAGCAGCA 
CCTCAGACAC CAAAGACTGT TCCCCCCCAG CCTGTAGGCA ATGCAGGGCA AGGGAAAGCA 
GCTGTGAATG CCACTAGCTC CCTCGCCAAA GCCTCCGTGA AAGCAGCCCC AGCTGACAGC 
TCCAGCAGTG ACAGCAGTGA CTCTGACACA GACGTCGAGC AGGTGTCTGC AAACCACAAA 
GCAGAAAGCA AACCCCCTCC GGACGAGGAC GCCACAGGAG CATCCAAAAA AGAGAAGGGG 
GCAGGAAAAA TTGATGTAAG TCATGCCAAC GCCAAGCCTT CGCCAGTCAA GAAAGCCCTG 
GCTGCAAAAG AGAATGATGT TGAGGCAAAA GAGCTGCAAG CGAACCCATT GCCATCCGCA 
CTGCTTTCAA CCCCACAGCC AGAGGAGTCA AGTCTGGGGA GCAAAAGTGA GGTCACCACT 
GCTGCTGGAG TTCCCGCAGT GCAAACACCA GAAGGGTTGG AAGAGAAGAA GAAGAAAAAA 
AAAGAGAAAA AAGAAAAGAA GGAAAAGAAG AAACATTCCT CCACAGCAGA CAAGGCTGTG 
AAAACATCTA AGGGCAAAGA CAAAGAGAAC AAAAAACAGA AAGCATCTCA GAAACGGAAA 
CGGCCAGGAG AGGATGGAGC TGTTGGGCAG CCCAAGGAAA AGAAGCGGAA AGGGCAAACT 
AGTGAAGAAG TGCCCAAGAA GAAGAAAAAG AAAGCAGCTG ATGACCCGGA GAAGCTGCCA 
GGCAGCAAGG AAAAGAAAAA ATCAAGTAAA AAGAAAAAGG CAGGAAAAGA AAAGAAGAAC 
AAAAAGATGC CCACAGAAGG GGAGTCGACA GCAGATGGCT CTGCTGAAGT CCACAAGAAG 
AAGAAGAAGA AGAAGAAAAC AGCTGAGCCC GAAGGATTGT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012803.2
CDS	76..3000
Translation	MAAEGRGRRELLALIHQHLLRAGYARAARELQAQLGQKLLPSLATSLEEIFTHWEKTPSKARRRKLSDEEAAIPEKIRVPDPVSSSESSEKEEDEKEKTKTGNATSITPVTNSAVNVESSEDDDSSSEEEMPSGKGAITVTPAVASGKAANSLHSPVKPTASGSKAAVPCAANRTVLSNKQQPSASVVTPAKAGQKLPSPGKPGRDAAAVSQAGQSKAPVMTKAPESSSSSSSSESEEEKETPTVKAPAPKVELKQAAGESESSSEESSDETSSEEELATAAVQAKPAVKAVQVNSTPVKSAPTTSVSTKKSTVKKRAVTLNQTKHGSVTVPVAAKPDDTSESSDSSDSENEEPPSVTQSKPSLKTSQAILSPVKTTPAASISSKAAPAKTLPLSQGKPTPKPAVLKQAKATPGRTAAPAKPAESANPAESTDSSGSEEDLPVPVSQKRLPEQPGPVPSLNQAAKAMTPEKAVGSTLKNETSESGSSDSSDSEEETPVSQTQPALPAAKSNAVPQPTSVKKVAAPTPAPAPPLHMDSSDDSSEESDFDEEIVPPTQAVPPPCPKQTAPMGKAPASPAAPHAASILRSPTAKSKKPGQQPAQDARLSQTAPLTQAEESSDSSSSSDSDEETSKQPPKPGMLQKPGGTEANDSSSSDSSEDEEAVSQSLLTGYMSLSKSPAAPQTPKTVPPQPVGNAGQGKAAVNATSSLAKASVKAAPADSSSSDSSDSDTDVEQVSANHKAESKPPPDEDATGASKKEKGAGKIDVSHANAKPSPVKKALAAKENDVEAKELQANPLPSALLSTPQPEESSLGSKSEVTTAAGVPAVQTPEGLEEKKKKKKEKKEKKEKKKHSSTADKAVKTSKGKDKENKKQKASQKRKRPGEDGAVGQPKEKKRKGQTSEEVPKKKKKKAADDPEKLPGSKEKKKSSKKKKAGKEKKNKKMPTEGESTADGSAEVHKKKKKKKKTAEPEGLL

Target ORF information:

RefSeq Version	NM_001012785.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Treacher Collins-Franceschetti syndrome 1 (TCOF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012785.2

ORF Insert Sequence:

ATGGCGGCGG AGGGCCGCGG GCGACGGGAG CTGCTGGCCC TCATCCACCA GCACCTCCTG 
CGCGCCGGCT ACGCGCGAGC CGCCCGCGAG CTACAGGCGC AGCTCGGACA GAAGCTGCTC 
CCGTCCCTGG CGACGTCGCT GGAGGAGATC TTCACGCACT GGGAGAAAAC TCCTTCGAAA 
GCCAGGAGAA GAAAGCTGAG TGATGAGGAG GCAGCCATCC CAGAAAAGAT CAGAGTTCCT 
GATCCTGTGA GCAGCTCTGA AAGCTCAGAG AAAGAAGAGG ATGAGAAAGA GAAGACGAAA 
ACTGGAAATG CAACCAGCAT TACCCCGGTC ACGAACTCAG CAGTGAATGT AGAAAGCAGT 
GAAGATGATG ACTCCTCATC AGAAGAAGAA ATGCCAAGTG GAAAAGGTGC AATTACGGTG 
ACACCAGCTG TAGCGAGTGG CAAAGCTGCC AACTCCTTGC ATTCTCCTGT TAAACCCACT 
GCTTCGGGAA GTAAAGCAGC GGTTCCCTGT GCTGCAAACA GAACTGTGCT CTCAAATAAG 
CAGCAGCCCA GTGCATCAGT TGTGACCCCA GCCAAAGCTG GGCAGAAACT GCCTAGTCCT 
GGGAAACCTG GACGAGATGC AGCAGCCGTG AGCCAAGCAG GACAAAGCAA AGCTCCAGTA 
ATGACCAAAG CACCGGAAAG CTCCAGCAGC AGCAGCTCGT CAGAGAGTGA GGAGGAAAAG 
GAGACACCGA CTGTGAAGGC CCCAGCCCCC AAAGTGGAGC TGAAACAGGC GGCTGGGGAA 
TCTGAGAGCA GCAGTGAGGA ATCAAGTGAT GAGACATCGT CTGAGGAGGA GCTTGCGACC 
GCAGCAGTCC AGGCAAAACC AGCTGTTAAA GCAGTGCAGG TTAATTCAAC ACCTGTGAAA 
AGTGCACCTA CTACTTCAGT GTCCACCAAG AAGAGCACAG TGAAAAAAAG AGCAGTGACA 
CTGAACCAGA CCAAGCATGG CTCAGTAACG GTTCCAGTGG CTGCAAAGCC TGATGACACC 
TCAGAGAGCA GCGACTCATC AGACAGTGAA AATGAGGAGC CTCCATCAGT AACCCAGTCA 
AAGCCATCTT TAAAAACCTC CCAGGCCATC CTATCCCCAG TGAAAACCAC ACCAGCAGCC 
TCAATCTCCA GCAAAGCAGC TCCAGCAAAA ACACTTCCAC TGTCCCAAGG GAAGCCAACG 
CCGAAACCGG CGGTGCTAAA GCAGGCTAAA GCCACACCAG GAAGGACTGC TGCTCCTGCA 
AAGCCAGCTG AAAGTGCAAA CCCAGCGGAG AGCACTGACT CTTCAGGCAG TGAAGAGGAT 
CTGCCTGTGC CTGTGAGCCA GAAGAGGCTA CCAGAACAGC CTGGGCCCGT TCCCAGCCTG 
AACCAGGCTG CTAAAGCCAT GACTCCTGAA AAGGCAGTGG GAAGCACCTT GAAGAATGAG 
ACCTCCGAAA GTGGAAGCAG TGACTCGTCT GACAGTGAGG AGGAGACTCC AGTGTCACAG 
ACACAGCCTG CACTGCCTGC TGCGAAAAGC AATGCTGTGC CCCAGCCAAC AAGCGTGAAG 
AAAGTAGCGG CTCCCACACC AGCCCCAGCC CCTCCTCTGC ATATGGACAG CAGTGATGAC 
TCCAGTGAGG AGTCGGATTT TGATGAGGAA ATAGTCCCCC CCACACAGGC TGTTCCTCCT 
CCTTGCCCAA AGCAGACAGC TCCCATGGGG AAGGCTCCAG CCAGCCCTGC TGCCCCGCAT 
GCTGCTTCCA TCCTGAGAAG CCCCACTGCC AAGTCGAAGA AGCCAGGCCA GCAGCCAGCA 
CAGGATGCCA GGCTGAGCCA GACTGCACCG CTCACCCAAG CAGAGGAGAG CTCGGACAGC 
AGCAGTTCCT CTGACAGCGA CGAGGAGACA TCCAAGCAGC CTCCCAAACC TGGGATGCTG 
CAGAAACCAG GAGGGACTGA GGCAAATGAC AGCTCCTCAT CGGATTCCAG TGAGGACGAA 
GAGGCAGTAT CACAGTCCCT CCTCACAGGT TATATGAGCC TCTCCAAGAG CCCAGCAGCA 
CCTCAGACAC CAAAGACTGT TCCCCCCCAG CCTGTAGGCA ATGCAGGGCA AGGGAAAGCA 
GCTGTGAATG CCACTAGCTC CCTCGCCAAA GCCTCCGTGA AAGCAGCCCC AGCTGACAGC 
TCCAGCAGTG ACAGCAGTGA CTCTGACACA GACGTCGAGC AGGTGTCTGC AAACCACAAA 
GCAGAAAGCA AACCCCCTCC GGACGAGGAC GCCACAGGAG CATCCAAAAA AGAGAAGGGG 
GCAGGAAAAA TTGATGTAAG TCATGCCAAC GCCAAGCCTT CGCCAGTCAA GAAAGCCCTG 
GCTGCAAAAG AGAATGATGT TGAGGCAAAA GAGCTGCAAG CGAACCCATT GCCATCCGCA 
CTGCTTTCAA CCCCACAGCC AGAGGAGTCA AGTCTGGGGA GCAAAAGTGA GGTCACCACT 
GCTGCTGGAG TTCCCGCAGT GCAAACACCA GAAGGGTTGG AAGAGAAGAA GAAGAAAAAA 
AAAGAGAAAA AAGAAAAGAA GGAAAAGAAG AAACATTCCT CCACAGCAGA CAAGGCTGTG 
AAAACATCTA AGGGCAAAGA CAAAGAGAAC AAAAAACAGA AAGCATCTCA GAAACGGAAA 
CGGCCAGGAG AGGATGGAGC TGTTGGGCAG CCCAAGGAAA AGAAGCGGAA AGGGCAAACT 
AGTGAAGAAG TGCCCAAGAA GAAGAAAAAG AAAGCAGCTG ATGACCCGGA GAAGCTGCCA 
GGCAGCAAGG AAAAGAAAAA ATCAAGTAAA AAGAAAAAGG CAGGAAAAGA AAAGAAGAAC 
AAAAAGATGC CCACAGAAGG GGAGTCGACA GCAGATGGCT CTGCTGAAGT CCACAAGAAG 
AAGAAGAAGA AGAAGAAAAC AGCTGAGCCC GAAGGATTGT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_015293786.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015149272.1 Gallus gallus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa41927
Clone ID Related Accession (Same CDS sequence)	XM_015293786.1 , XM_015293787.1
Accession Version	XM_015293786.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	treacle protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006100.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGGGCCGCGG GCGACGGGAG CTGCTGGCCC TCATCCACCA GCACCTCCTG 
CGCGCCGGCT ACGCGCGAGC CGCCCGCGAG CTACAGGCGC AGCTCGGACA GAAGCTGCTC 
CCGTCCCTGG CGACGTCGCT GGAGGAGATC TTCACGCACT GGGAGAAAAC TCCTTCGAAA 
GCCAGGAGAA GAAAGCTGAG TGATGAGGAG GCAGCCATCC CAGAAAAGAT CAGAGTTCCT 
GATCCTGTGA GCAGCTCTGA AAGCTCAGAG AAAGAAGAGG ATGAGAAAGA GAAGACGAAA 
ACTGGAAATG CAACCAGCAT TACCCCGGTC ACGAACTCAG CAGTGAATGT AGAAAGCAGT 
GAAGATGATG ACTCCTCATC AGAAGAAGAA ATGCCAAGTG GAAAAGGTGC AATTACGGTG 
ACACCAGCTG TAGCGAGTGG CAAAGCTGCC AACTCCTTGC ATTCTCCTGT TAAACCCACT 
GCTTCGGGAA GTAAAGCAGC GGTTCCCTGT GCTGCAAACA GAACTGTGCT CTCAAATAAG 
CAGCAGCCCA GTGCATCAGT TGTGACCCCA GCCAAAGCTG GGCAGAAACT GCCTAGTCCT 
GGGAAACCTG GACGAGATGC AGCAGCCGTG AGCCAAGCAG GACAAAGCAA AGCTCCAGTA 
ATGACCAAAG CACCGGAAAG CTCCAGCAGC AGCAGCTCGT CAGAGAGTGA GGAGGAAAAG 
GAGACACCGA CTGTGAAGGC CCCAGCCCCC AAAGTGGAGC TGAAACAGGC GGCTGGGGAA 
TCTGAGAGCA GCAGTGAGGA ATCAAGTGAT GAGACATCGT CTGAGGAGGA GCTTGCGACC 
GCAGCAGTCC AGGCAAAACC AGCTGTTAAA GCAGTGCAGG TTAATTCAAC ACCTGTGAAA 
AGTGCACCTA CTACTTCAGT GTCCACCAAG AAGAGCACAG TGAAAAAAAG AGCAGTGACA 
CTGAACCAGA CCAAGCATGG CTCAGTAACG GTTCCAGTGG CTGCAAAGCC TGATGACACC 
TCAGAGAGCA GCGACTCATC AGACAGTGAA AATGAGGAGC CTCCATCAGT AACCCAGTCA 
AAGCCATCTT TAAAAACCTC CCAGGCCATC CTATCCCCAG TGAAAACCAC ACCAGCAGCC 
TCAATCTCCA GCAAAGCAGC TCCAGCAAAA ACACTTCCAC TGTCCCAAGG GAAGCCAACG 
CCGAAACCGG CGGTGCTAAA GCAGGCTAAA GCCACACCAG GAAGGACTGC TGCTCCTGCA 
AAGCCAGCTG AAAGTGCAAA CCCAGCGGAG AGCACTGACT CTTCAGGCAG TGAAGAGGAT 
CTGCCTGTGC CTGTGAGCCA GAAGAGGCTA CCAGAACAGC CTGGGCCCGT TCCCAGCCTG 
AACCAGGCTG CTAAAGCCAT GACTCCTGAA AAGGCAGTGG GAAGCACCTT GAAGAATGAG 
ACCTCCGAAA GTGGAAGCAG TGACTCGTCT GACAGTGAGG AGGAGACTCC AGTGTCACAG 
ACACAGCCTG CACTGCCTGC TGCGAAAAGC AATGCTGTGC CCCAGCCAAC AAGCGTGAAG 
AAAGTAGCGG CTCCCACACC AGCCCCAGCC CCTCCTCTGC ATATGGACAG CAGTGATGAC 
TCCAGTGAGG AGTCGGATTC TGATGAGGAA ATAGTCCCCC CCACACAGAC TCTATCCCAG 
CAGAACATCA AACCAACCAA GGTTTCAAGC ACAGGGGCCG CCAAGGCTAA TGCCACACCA 
CCTTCAGGGA AGGGAATAAA AGCACCTCCT ATCCCTCCGA TTTCCAGAGT GTCTGAGAGC 
TCAAACAGTG ATTCCTCAGA GAGCAGTACA GTGGTTGGAG AGGCTGTTCC TCCTCCTTGC 
CCAAAGCAGA CAGCTCCCAT GGGGAAGGCT CCAGCCAGCC CTGCTGCCCC GCATGCTGCT 
TCCATCCTGA GAAGCCCCAC TGCCAAGTCG AAGAAGCCAG GCCAGCAGCC AGCACAGGAT 
GCCAGGCTGA GCCAGACTGC ACCGCTCACC CAAGCAGAGG AGAGCTCGGA CAGCAGCAGT 
TCCTCTGACA GCGACGAGGA GACATCCAAG CAGCCTCCCA AACCTGGGAT GCTGCAGAAA 
CCAGGAGGGA CTGAGGCAAA TGACAGCTCC TCATCGGATT CCAGTGAGGA CGAAGAGGCA 
GTATCACAGT CCCTCCTCAC AGGTTATATG AGCCTCTCCA AGAGCCCAGC AGCACCTCAG 
ACACCAAAGA CTGTTCCCCC CCAGCCTGTA GGCAATGCAG GGCAAGGGAA AGCAGCTGTG 
AATGCCACTA GCTCCCTCGC CAAAGCCTCC GTGAAAGCAG CCCCAGCTGA CAGCTCCAGC 
AGTGACAGCA GTGACTCTGA CACAGACGTC GAGCAGGTGT CTGCAAACCA CAAAGCAGAA 
AGCAAACCCC CTCCGGACGA GGACGCCACA GGAGCATCCA AAAAAGAGAA GGGGGCAGGA 
AAAATTGATG TAAGTCATGC CAACGCCAAG CCTTCGCCAG TCAAGAAAGC CCTGGCTGCA 
AAAGAGAATG ATGTTGAGGC AAAAGAGCTG CAAGCGAACC CATTGCCATC CGCACTGCTT 
TCAACCCCAC AGCCAGAGGA GTCAAGTCTG GGGAGCAAAA GTGAGGTCAC CACTGCTGCT 
GGAGTTCCCG CAGTGCAAAC ACCAGAAGGG TTGGAAGAGA AGAAGAAGAA AAAAAAAGAG 
AAAAAAGAAA AGAAGGAAAA GAAGAAACAT TCCTCCACAG CAGACAAGGC TGTGAAAACA 
TCTAAGGGCA AAGACAAAGA GAACAAAAAA CAGAAAGCAT CTCAGAAACG GAAACGGCCA 
GGAGAGGATG GAGCTGTTGG GCAGCCCAAG GAAAAGAAGC GGAAAGGGCA AACTAGTGAA 
GAAGTGCCCA AGAAGAAGAA AAAGAAAGCA GCTGATGACC CGGAGAAGCT GCCAGGCAGC 
AAGGAAAAGA AAAAATCAAG TAAAAAGAAA AAGGCAGGAA AAGAAAAGAA GAACAAAAAG 
ATGCCCACAG AAGGGGAGTC GACAGCAGAT GGCTCTGCTG AAGTCCACAA GAAGAAGAAG 
AAGAAGAAGA AAACAGCTGA GCCCGAAGGA TTGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015149272.1
CDS	71..3169
Translation	MAAEGRGRRELLALIHQHLLRAGYARAARELQAQLGQKLLPSLATSLEEIFTHWEKTPSKARRRKLSDEEAAIPEKIRVPDPVSSSESSEKEEDEKEKTKTGNATSITPVTNSAVNVESSEDDDSSSEEEMPSGKGAITVTPAVASGKAANSLHSPVKPTASGSKAAVPCAANRTVLSNKQQPSASVVTPAKAGQKLPSPGKPGRDAAAVSQAGQSKAPVMTKAPESSSSSSSSESEEEKETPTVKAPAPKVELKQAAGESESSSEESSDETSSEEELATAAVQAKPAVKAVQVNSTPVKSAPTTSVSTKKSTVKKRAVTLNQTKHGSVTVPVAAKPDDTSESSDSSDSENEEPPSVTQSKPSLKTSQAILSPVKTTPAASISSKAAPAKTLPLSQGKPTPKPAVLKQAKATPGRTAAPAKPAESANPAESTDSSGSEEDLPVPVSQKRLPEQPGPVPSLNQAAKAMTPEKAVGSTLKNETSESGSSDSSDSEEETPVSQTQPALPAAKSNAVPQPTSVKKVAAPTPAPAPPLHMDSSDDSSEESDSDEEIVPPTQTLSQQNIKPTKVSSTGAAKANATPPSGKGIKAPPIPPISRVSESSNSDSSESSTVVGEAVPPPCPKQTAPMGKAPASPAAPHAASILRSPTAKSKKPGQQPAQDARLSQTAPLTQAEESSDSSSSSDSDEETSKQPPKPGMLQKPGGTEANDSSSSDSSEDEEAVSQSLLTGYMSLSKSPAAPQTPKTVPPQPVGNAGQGKAAVNATSSLAKASVKAAPADSSSSDSSDSDTDVEQVSANHKAESKPPPDEDATGASKKEKGAGKIDVSHANAKPSPVKKALAAKENDVEAKELQANPLPSALLSTPQPEESSLGSKSEVTTAAGVPAVQTPEGLEEKKKKKKEKKEKKEKKKHSSTADKAVKTSKGKDKENKKQKASQKRKRPGEDGAVGQPKEKKRKGQTSEEVPKKKKKKAADDPEKLPGSKEKKKSSKKKKAGKEKKNKKMPTEGESTADGSAEVHKKKKKKKKTAEPEGLL

Target ORF information:

RefSeq Version	XM_015293786.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293786.1

ORF Insert Sequence:

ATGGCGGCGG AGGGCCGCGG GCGACGGGAG CTGCTGGCCC TCATCCACCA GCACCTCCTG 
CGCGCCGGCT ACGCGCGAGC CGCCCGCGAG CTACAGGCGC AGCTCGGACA GAAGCTGCTC 
CCGTCCCTGG CGACGTCGCT GGAGGAGATC TTCACGCACT GGGAGAAAAC TCCTTCGAAA 
GCCAGGAGAA GAAAGCTGAG TGATGAGGAG GCAGCCATCC CAGAAAAGAT CAGAGTTCCT 
GATCCTGTGA GCAGCTCTGA AAGCTCAGAG AAAGAAGAGG ATGAGAAAGA GAAGACGAAA 
ACTGGAAATG CAACCAGCAT TACCCCGGTC ACGAACTCAG CAGTGAATGT AGAAAGCAGT 
GAAGATGATG ACTCCTCATC AGAAGAAGAA ATGCCAAGTG GAAAAGGTGC AATTACGGTG 
ACACCAGCTG TAGCGAGTGG CAAAGCTGCC AACTCCTTGC ATTCTCCTGT TAAACCCACT 
GCTTCGGGAA GTAAAGCAGC GGTTCCCTGT GCTGCAAACA GAACTGTGCT CTCAAATAAG 
CAGCAGCCCA GTGCATCAGT TGTGACCCCA GCCAAAGCTG GGCAGAAACT GCCTAGTCCT 
GGGAAACCTG GACGAGATGC AGCAGCCGTG AGCCAAGCAG GACAAAGCAA AGCTCCAGTA 
ATGACCAAAG CACCGGAAAG CTCCAGCAGC AGCAGCTCGT CAGAGAGTGA GGAGGAAAAG 
GAGACACCGA CTGTGAAGGC CCCAGCCCCC AAAGTGGAGC TGAAACAGGC GGCTGGGGAA 
TCTGAGAGCA GCAGTGAGGA ATCAAGTGAT GAGACATCGT CTGAGGAGGA GCTTGCGACC 
GCAGCAGTCC AGGCAAAACC AGCTGTTAAA GCAGTGCAGG TTAATTCAAC ACCTGTGAAA 
AGTGCACCTA CTACTTCAGT GTCCACCAAG AAGAGCACAG TGAAAAAAAG AGCAGTGACA 
CTGAACCAGA CCAAGCATGG CTCAGTAACG GTTCCAGTGG CTGCAAAGCC TGATGACACC 
TCAGAGAGCA GCGACTCATC AGACAGTGAA AATGAGGAGC CTCCATCAGT AACCCAGTCA 
AAGCCATCTT TAAAAACCTC CCAGGCCATC CTATCCCCAG TGAAAACCAC ACCAGCAGCC 
TCAATCTCCA GCAAAGCAGC TCCAGCAAAA ACACTTCCAC TGTCCCAAGG GAAGCCAACG 
CCGAAACCGG CGGTGCTAAA GCAGGCTAAA GCCACACCAG GAAGGACTGC TGCTCCTGCA 
AAGCCAGCTG AAAGTGCAAA CCCAGCGGAG AGCACTGACT CTTCAGGCAG TGAAGAGGAT 
CTGCCTGTGC CTGTGAGCCA GAAGAGGCTA CCAGAACAGC CTGGGCCCGT TCCCAGCCTG 
AACCAGGCTG CTAAAGCCAT GACTCCTGAA AAGGCAGTGG GAAGCACCTT GAAGAATGAG 
ACCTCCGAAA GTGGAAGCAG TGACTCGTCT GACAGTGAGG AGGAGACTCC AGTGTCACAG 
ACACAGCCTG CACTGCCTGC TGCGAAAAGC AATGCTGTGC CCCAGCCAAC AAGCGTGAAG 
AAAGTAGCGG CTCCCACACC AGCCCCAGCC CCTCCTCTGC ATATGGACAG CAGTGATGAC 
TCCAGTGAGG AGTCGGATTC TGATGAGGAA ATAGTCCCCC CCACACAGAC TCTATCCCAG 
CAGAACATCA AACCAACCAA GGTTTCAAGC ACAGGGGCCG CCAAGGCTAA TGCCACACCA 
CCTTCAGGGA AGGGAATAAA AGCACCTCCT ATCCCTCCGA TTTCCAGAGT GTCTGAGAGC 
TCAAACAGTG ATTCCTCAGA GAGCAGTACA GTGGTTGGAG AGGCTGTTCC TCCTCCTTGC 
CCAAAGCAGA CAGCTCCCAT GGGGAAGGCT CCAGCCAGCC CTGCTGCCCC GCATGCTGCT 
TCCATCCTGA GAAGCCCCAC TGCCAAGTCG AAGAAGCCAG GCCAGCAGCC AGCACAGGAT 
GCCAGGCTGA GCCAGACTGC ACCGCTCACC CAAGCAGAGG AGAGCTCGGA CAGCAGCAGT 
TCCTCTGACA GCGACGAGGA GACATCCAAG CAGCCTCCCA AACCTGGGAT GCTGCAGAAA 
CCAGGAGGGA CTGAGGCAAA TGACAGCTCC TCATCGGATT CCAGTGAGGA CGAAGAGGCA 
GTATCACAGT CCCTCCTCAC AGGTTATATG AGCCTCTCCA AGAGCCCAGC AGCACCTCAG 
ACACCAAAGA CTGTTCCCCC CCAGCCTGTA GGCAATGCAG GGCAAGGGAA AGCAGCTGTG 
AATGCCACTA GCTCCCTCGC CAAAGCCTCC GTGAAAGCAG CCCCAGCTGA CAGCTCCAGC 
AGTGACAGCA GTGACTCTGA CACAGACGTC GAGCAGGTGT CTGCAAACCA CAAAGCAGAA 
AGCAAACCCC CTCCGGACGA GGACGCCACA GGAGCATCCA AAAAAGAGAA GGGGGCAGGA 
AAAATTGATG TAAGTCATGC CAACGCCAAG CCTTCGCCAG TCAAGAAAGC CCTGGCTGCA 
AAAGAGAATG ATGTTGAGGC AAAAGAGCTG CAAGCGAACC CATTGCCATC CGCACTGCTT 
TCAACCCCAC AGCCAGAGGA GTCAAGTCTG GGGAGCAAAA GTGAGGTCAC CACTGCTGCT 
GGAGTTCCCG CAGTGCAAAC ACCAGAAGGG TTGGAAGAGA AGAAGAAGAA AAAAAAAGAG 
AAAAAAGAAA AGAAGGAAAA GAAGAAACAT TCCTCCACAG CAGACAAGGC TGTGAAAACA 
TCTAAGGGCA AAGACAAAGA GAACAAAAAA CAGAAAGCAT CTCAGAAACG GAAACGGCCA 
GGAGAGGATG GAGCTGTTGG GCAGCCCAAG GAAAAGAAGC GGAAAGGGCA AACTAGTGAA 
GAAGTGCCCA AGAAGAAGAA AAAGAAAGCA GCTGATGACC CGGAGAAGCT GCCAGGCAGC 
AAGGAAAAGA AAAAATCAAG TAAAAAGAAA AAGGCAGGAA AAGAAAAGAA GAACAAAAAG 
ATGCCCACAG AAGGGGAGTC GACAGCAGAT GGCTCTGCTG AAGTCCACAA GAAGAAGAAG 
AAGAAGAAGA AAACAGCTGA GCCCGAAGGA TTGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_015293787.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015149273.1 Gallus gallus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa41927
Clone ID Related Accession (Same CDS sequence)	XM_015293786.1 , XM_015293787.1
Accession Version	XM_015293787.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	treacle protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006100.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGGGCCGCGG GCGACGGGAG CTGCTGGCCC TCATCCACCA GCACCTCCTG 
CGCGCCGGCT ACGCGCGAGC CGCCCGCGAG CTACAGGCGC AGCTCGGACA GAAGCTGCTC 
CCGTCCCTGG CGACGTCGCT GGAGGAGATC TTCACGCACT GGGAGAAAAC TCCTTCGAAA 
GCCAGGAGAA GAAAGCTGAG TGATGAGGAG GCAGCCATCC CAGAAAAGAT CAGAGTTCCT 
GATCCTGTGA GCAGCTCTGA AAGCTCAGAG AAAGAAGAGG ATGAGAAAGA GAAGACGAAA 
ACTGGAAATG CAACCAGCAT TACCCCGGTC ACGAACTCAG CAGTGAATGT AGAAAGCAGT 
GAAGATGATG ACTCCTCATC AGAAGAAGAA ATGCCAAGTG GAAAAGGTGC AATTACGGTG 
ACACCAGCTG TAGCGAGTGG CAAAGCTGCC AACTCCTTGC ATTCTCCTGT TAAACCCACT 
GCTTCGGGAA GTAAAGCAGC GGTTCCCTGT GCTGCAAACA GAACTGTGCT CTCAAATAAG 
CAGCAGCCCA GTGCATCAGT TGTGACCCCA GCCAAAGCTG GGCAGAAACT GCCTAGTCCT 
GGGAAACCTG GACGAGATGC AGCAGCCGTG AGCCAAGCAG GACAAAGCAA AGCTCCAGTA 
ATGACCAAAG CACCGGAAAG CTCCAGCAGC AGCAGCTCGT CAGAGAGTGA GGAGGAAAAG 
GAGACACCGA CTGTGAAGGC CCCAGCCCCC AAAGTGGAGC TGAAACAGGC GGCTGGGGAA 
TCTGAGAGCA GCAGTGAGGA ATCAAGTGAT GAGACATCGT CTGAGGAGGA GCTTGCGACC 
GCAGCAGTCC AGGCAAAACC AGCTGTTAAA GCAGTGCAGG TTAATTCAAC ACCTGTGAAA 
AGTGCACCTA CTACTTCAGT GTCCACCAAG AAGAGCACAG TGAAAAAAAG AGCAGTGACA 
CTGAACCAGA CCAAGCATGG CTCAGTAACG GTTCCAGTGG CTGCAAAGCC TGATGACACC 
TCAGAGAGCA GCGACTCATC AGACAGTGAA AATGAGGAGC CTCCATCAGT AACCCAGTCA 
AAGCCATCTT TAAAAACCTC CCAGGCCATC CTATCCCCAG TGAAAACCAC ACCAGCAGCC 
TCAATCTCCA GCAAAGCAGC TCCAGCAAAA ACACTTCCAC TGTCCCAAGG GAAGCCAACG 
CCGAAACCGG CGGTGCTAAA GCAGGCTAAA GCCACACCAG GAAGGACTGC TGCTCCTGCA 
AAGCCAGCTG AAAGTGCAAA CCCAGCGGAG AGCACTGACT CTTCAGGCAG TGAAGAGGAT 
CTGCCTGTGC CTGTGAGCCA GAAGAGGCTA CCAGAACAGC CTGGGCCCGT TCCCAGCCTG 
AACCAGGCTG CTAAAGCCAT GACTCCTGAA AAGGCAGTGG GAAGCACCTT GAAGAATGAG 
ACCTCCGAAA GTGGAAGCAG TGACTCGTCT GACAGTGAGG AGGAGACTCC AGTGTCACAG 
ACACAGCCTG CACTGCCTGC TGCGAAAAGC AATGCTGTGC CCCAGCCAAC AAGCGTGAAG 
AAAGTAGCGG CTCCCACACC AGCCCCAGCC CCTCCTCTGC ATATGGACAG CAGTGATGAC 
TCCAGTGAGG AGTCGGATTC TGATGAGGAA ATAGTCCCCC CCACACAGAC TCTATCCCAG 
CAGAACATCA AACCAACCAA GGTTTCAAGC ACAGGGGCCG CCAAGGCTAA TGCCACACCA 
CCTTCAGGGA AGGGAATAAA AGCACCTCCT ATCCCTCCGA TTTCCAGAGT GTCTGAGAGC 
TCAAACAGTG ATTCCTCAGA GAGCAGTACA GTGGTTGGAG AGGCTGTTCC TCCTCCTTGC 
CCAAAGCAGA CAGCTCCCAT GGGGAAGGCT CCAGCCAGCC CTGCTGCCCC GCATGCTGCT 
TCCATCCTGA GAAGCCCCAC TGCCAAGTCG AAGAAGCCAG GCCAGCAGCC AGCACAGGAT 
GCCAGGCTGA GCCAGACTGC ACCGCTCACC CAAGCAGAGG AGAGCTCGGA CAGCAGCAGT 
TCCTCTGACA GCGACGAGGA GACATCCAAG CAGCCTCCCA AACCTGGGAT GCTGCAGAAA 
CCAGGAGGGA CTGAGGCAAA TGACAGCTCC TCATCGGATT CCAGTGAGGA CGAAGAGGCA 
GTATCACAGT CCCTCCTCAC AGGTTATATG AGCCTCTCCA AGAGCCCAGC AGCACCTCAG 
ACACCAAAGA CTGTTCCCCC CCAGCCTGTA GGCAATGCAG GGCAAGGGAA AGCAGCTGTG 
AATGCCACTA GCTCCCTCGC CAAAGCCTCC GTGAAAGCAG CCCCAGCTGA CAGCTCCAGC 
AGTGACAGCA GTGACTCTGA CACAGACGTC GAGCAGGTGT CTGCAAACCA CAAAGCAGAA 
AGCAAACCCC CTCCGGACGA GGACGCCACA GGAGCATCCA AAAAAGAGAA GGGGGCAGGA 
AAAATTGATG TAAGTCATGC CAACGCCAAG CCTTCGCCAG TCAAGAAAGC CCTGGCTGCA 
AAAGAGAATG ATGTTGAGGC AAAAGAGCTG CAAGCGAACC CATTGCCATC CGCACTGCTT 
TCAACCCCAC AGCCAGAGGA GTCAAGTCTG GGGAGCAAAA GTGAGGTCAC CACTGCTGCT 
GGAGTTCCCG CAGTGCAAAC ACCAGAAGGG TTGGAAGAGA AGAAGAAGAA AAAAAAAGAG 
AAAAAAGAAA AGAAGGAAAA GAAGAAACAT TCCTCCACAG CAGACAAGGC TGTGAAAACA 
TCTAAGGGCA AAGACAAAGA GAACAAAAAA CAGAAAGCAT CTCAGAAACG GAAACGGCCA 
GGAGAGGATG GAGCTGTTGG GCAGCCCAAG GAAAAGAAGC GGAAAGGGCA AACTAGTGAA 
GAAGTGCCCA AGAAGAAGAA AAAGAAAGCA GCTGATGACC CGGAGAAGCT GCCAGGCAGC 
AAGGAAAAGA AAAAATCAAG TAAAAAGAAA AAGGCAGGAA AAGAAAAGAA GAACAAAAAG 
ATGCCCACAG AAGGGGAGTC GACAGCAGAT GGCTCTGCTG AAGTCCACAA GAAGAAGAAG 
AAGAAGAAGA AAACAGCTGA GCCCGAAGGA TTGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015149273.1
CDS	71..3169
Translation	MAAEGRGRRELLALIHQHLLRAGYARAARELQAQLGQKLLPSLATSLEEIFTHWEKTPSKARRRKLSDEEAAIPEKIRVPDPVSSSESSEKEEDEKEKTKTGNATSITPVTNSAVNVESSEDDDSSSEEEMPSGKGAITVTPAVASGKAANSLHSPVKPTASGSKAAVPCAANRTVLSNKQQPSASVVTPAKAGQKLPSPGKPGRDAAAVSQAGQSKAPVMTKAPESSSSSSSSESEEEKETPTVKAPAPKVELKQAAGESESSSEESSDETSSEEELATAAVQAKPAVKAVQVNSTPVKSAPTTSVSTKKSTVKKRAVTLNQTKHGSVTVPVAAKPDDTSESSDSSDSENEEPPSVTQSKPSLKTSQAILSPVKTTPAASISSKAAPAKTLPLSQGKPTPKPAVLKQAKATPGRTAAPAKPAESANPAESTDSSGSEEDLPVPVSQKRLPEQPGPVPSLNQAAKAMTPEKAVGSTLKNETSESGSSDSSDSEEETPVSQTQPALPAAKSNAVPQPTSVKKVAAPTPAPAPPLHMDSSDDSSEESDSDEEIVPPTQTLSQQNIKPTKVSSTGAAKANATPPSGKGIKAPPIPPISRVSESSNSDSSESSTVVGEAVPPPCPKQTAPMGKAPASPAAPHAASILRSPTAKSKKPGQQPAQDARLSQTAPLTQAEESSDSSSSSDSDEETSKQPPKPGMLQKPGGTEANDSSSSDSSEDEEAVSQSLLTGYMSLSKSPAAPQTPKTVPPQPVGNAGQGKAAVNATSSLAKASVKAAPADSSSSDSSDSDTDVEQVSANHKAESKPPPDEDATGASKKEKGAGKIDVSHANAKPSPVKKALAAKENDVEAKELQANPLPSALLSTPQPEESSLGSKSEVTTAAGVPAVQTPEGLEEKKKKKKEKKEKKEKKKHSSTADKAVKTSKGKDKENKKQKASQKRKRPGEDGAVGQPKEKKRKGQTSEEVPKKKKKKAADDPEKLPGSKEKKKSSKKKKAGKEKKNKKMPTEGESTADGSAEVHKKKKKKKKTAEPEGLL

Target ORF information:

RefSeq Version	XM_015293787.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293787.1

ORF Insert Sequence:

ATGGCGGCGG AGGGCCGCGG GCGACGGGAG CTGCTGGCCC TCATCCACCA GCACCTCCTG 
CGCGCCGGCT ACGCGCGAGC CGCCCGCGAG CTACAGGCGC AGCTCGGACA GAAGCTGCTC 
CCGTCCCTGG CGACGTCGCT GGAGGAGATC TTCACGCACT GGGAGAAAAC TCCTTCGAAA 
GCCAGGAGAA GAAAGCTGAG TGATGAGGAG GCAGCCATCC CAGAAAAGAT CAGAGTTCCT 
GATCCTGTGA GCAGCTCTGA AAGCTCAGAG AAAGAAGAGG ATGAGAAAGA GAAGACGAAA 
ACTGGAAATG CAACCAGCAT TACCCCGGTC ACGAACTCAG CAGTGAATGT AGAAAGCAGT 
GAAGATGATG ACTCCTCATC AGAAGAAGAA ATGCCAAGTG GAAAAGGTGC AATTACGGTG 
ACACCAGCTG TAGCGAGTGG CAAAGCTGCC AACTCCTTGC ATTCTCCTGT TAAACCCACT 
GCTTCGGGAA GTAAAGCAGC GGTTCCCTGT GCTGCAAACA GAACTGTGCT CTCAAATAAG 
CAGCAGCCCA GTGCATCAGT TGTGACCCCA GCCAAAGCTG GGCAGAAACT GCCTAGTCCT 
GGGAAACCTG GACGAGATGC AGCAGCCGTG AGCCAAGCAG GACAAAGCAA AGCTCCAGTA 
ATGACCAAAG CACCGGAAAG CTCCAGCAGC AGCAGCTCGT CAGAGAGTGA GGAGGAAAAG 
GAGACACCGA CTGTGAAGGC CCCAGCCCCC AAAGTGGAGC TGAAACAGGC GGCTGGGGAA 
TCTGAGAGCA GCAGTGAGGA ATCAAGTGAT GAGACATCGT CTGAGGAGGA GCTTGCGACC 
GCAGCAGTCC AGGCAAAACC AGCTGTTAAA GCAGTGCAGG TTAATTCAAC ACCTGTGAAA 
AGTGCACCTA CTACTTCAGT GTCCACCAAG AAGAGCACAG TGAAAAAAAG AGCAGTGACA 
CTGAACCAGA CCAAGCATGG CTCAGTAACG GTTCCAGTGG CTGCAAAGCC TGATGACACC 
TCAGAGAGCA GCGACTCATC AGACAGTGAA AATGAGGAGC CTCCATCAGT AACCCAGTCA 
AAGCCATCTT TAAAAACCTC CCAGGCCATC CTATCCCCAG TGAAAACCAC ACCAGCAGCC 
TCAATCTCCA GCAAAGCAGC TCCAGCAAAA ACACTTCCAC TGTCCCAAGG GAAGCCAACG 
CCGAAACCGG CGGTGCTAAA GCAGGCTAAA GCCACACCAG GAAGGACTGC TGCTCCTGCA 
AAGCCAGCTG AAAGTGCAAA CCCAGCGGAG AGCACTGACT CTTCAGGCAG TGAAGAGGAT 
CTGCCTGTGC CTGTGAGCCA GAAGAGGCTA CCAGAACAGC CTGGGCCCGT TCCCAGCCTG 
AACCAGGCTG CTAAAGCCAT GACTCCTGAA AAGGCAGTGG GAAGCACCTT GAAGAATGAG 
ACCTCCGAAA GTGGAAGCAG TGACTCGTCT GACAGTGAGG AGGAGACTCC AGTGTCACAG 
ACACAGCCTG CACTGCCTGC TGCGAAAAGC AATGCTGTGC CCCAGCCAAC AAGCGTGAAG 
AAAGTAGCGG CTCCCACACC AGCCCCAGCC CCTCCTCTGC ATATGGACAG CAGTGATGAC 
TCCAGTGAGG AGTCGGATTC TGATGAGGAA ATAGTCCCCC CCACACAGAC TCTATCCCAG 
CAGAACATCA AACCAACCAA GGTTTCAAGC ACAGGGGCCG CCAAGGCTAA TGCCACACCA 
CCTTCAGGGA AGGGAATAAA AGCACCTCCT ATCCCTCCGA TTTCCAGAGT GTCTGAGAGC 
TCAAACAGTG ATTCCTCAGA GAGCAGTACA GTGGTTGGAG AGGCTGTTCC TCCTCCTTGC 
CCAAAGCAGA CAGCTCCCAT GGGGAAGGCT CCAGCCAGCC CTGCTGCCCC GCATGCTGCT 
TCCATCCTGA GAAGCCCCAC TGCCAAGTCG AAGAAGCCAG GCCAGCAGCC AGCACAGGAT 
GCCAGGCTGA GCCAGACTGC ACCGCTCACC CAAGCAGAGG AGAGCTCGGA CAGCAGCAGT 
TCCTCTGACA GCGACGAGGA GACATCCAAG CAGCCTCCCA AACCTGGGAT GCTGCAGAAA 
CCAGGAGGGA CTGAGGCAAA TGACAGCTCC TCATCGGATT CCAGTGAGGA CGAAGAGGCA 
GTATCACAGT CCCTCCTCAC AGGTTATATG AGCCTCTCCA AGAGCCCAGC AGCACCTCAG 
ACACCAAAGA CTGTTCCCCC CCAGCCTGTA GGCAATGCAG GGCAAGGGAA AGCAGCTGTG 
AATGCCACTA GCTCCCTCGC CAAAGCCTCC GTGAAAGCAG CCCCAGCTGA CAGCTCCAGC 
AGTGACAGCA GTGACTCTGA CACAGACGTC GAGCAGGTGT CTGCAAACCA CAAAGCAGAA 
AGCAAACCCC CTCCGGACGA GGACGCCACA GGAGCATCCA AAAAAGAGAA GGGGGCAGGA 
AAAATTGATG TAAGTCATGC CAACGCCAAG CCTTCGCCAG TCAAGAAAGC CCTGGCTGCA 
AAAGAGAATG ATGTTGAGGC AAAAGAGCTG CAAGCGAACC CATTGCCATC CGCACTGCTT 
TCAACCCCAC AGCCAGAGGA GTCAAGTCTG GGGAGCAAAA GTGAGGTCAC CACTGCTGCT 
GGAGTTCCCG CAGTGCAAAC ACCAGAAGGG TTGGAAGAGA AGAAGAAGAA AAAAAAAGAG 
AAAAAAGAAA AGAAGGAAAA GAAGAAACAT TCCTCCACAG CAGACAAGGC TGTGAAAACA 
TCTAAGGGCA AAGACAAAGA GAACAAAAAA CAGAAAGCAT CTCAGAAACG GAAACGGCCA 
GGAGAGGATG GAGCTGTTGG GCAGCCCAAG GAAAAGAAGC GGAAAGGGCA AACTAGTGAA 
GAAGTGCCCA AGAAGAAGAA AAAGAAAGCA GCTGATGACC CGGAGAAGCT GCCAGGCAGC 
AAGGAAAAGA AAAAATCAAG TAAAAAGAAA AAGGCAGGAA AAGAAAAGAA GAACAAAAAG 
ATGCCCACAG AAGGGGAGTC GACAGCAGAT GGCTCTGCTG AAGTCCACAA GAAGAAGAAG 
AAGAAGAAGA AAACAGCTGA GCCCGAAGGA TTGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_015293789.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015149275.1 Gallus gallus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa41928
Clone ID Related Accession (Same CDS sequence)	XM_015293789.1 , XM_015293789.2
Accession Version	XM_015293789.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	treacle protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455956.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCTG CAGAGGTGTC TCCTGGTAGG ATGCCTCATG CCTTGGTGCC TTGGTCTTGT 
TCCTGCAAGA GCAGGAGAAA GCCTGGATCT AGAACTCCTT CGAAAGCCAG GAGAAGAAAG 
CTGAGTGATG AGGAGGCAGC CATCCCAGAA AAGATCAGAG TTCCTGATCC TGTGAGCAGC 
TCTGAAAGCT CAGAGAAAGA AGAGGATGAG AAAGAGAAGA CGAAAACTGG AAATGCAACC 
AGCATTACCC CGGTCACGAA CTCAGCAGTG AATGTAGAAA GCAGTGAAGA TGATGACTCC 
TCATCAGAAG AAGAAATGCC AAGTGGAAAA GGTGCAATTA CGGTGACACC AGCTGTAGCG 
AGTGGCAAAG CTGCCAACTC CTTGCATTCT CCTGTTAAAC CCACTGCTTC GGGAAGTAAA 
GCAGCGGTTC CCTGTGCTGC AAACAGAACT GTGCTCTCAA ATAAGCAGCA GCCCAGTGCA 
TCAGTTGTGA CCCCAGCCAA AGCTGGGCAG AAACTGCCTA GTCCTGGGAA ACCTGGACGA 
GATGCAGCAG CCGTGAGCCA AGCAGGACAA AGCAAAGCTC CAGTAATGAC CAAAGCACCG 
GAAAGCTCCA GCAGCAGCAG CTCGTCAGAG AGTGAGGAGG AAAAGGAGAC ACCGACTGTG 
AAGGCCCCAG CCCCCAAAGT GGAGCTGAAA CAGGCGGCTG GGGAATCTGA GAGCAGCAGT 
GAGGAATCAA GTGATGAGAC ATCGTCTGAG GAGGAGCTTG CGACCGCAGC AGTCCAGGCA 
AAACCAGCTG TTAAAGCAGT GCAGGTTAAT TCAACACCTG TGAAAAGTGC ACCTACTACT 
TCAGTGTCCA CCAAGAAGAG CACAGTGAAA AAAAGAGCAG TGACACTGAA CCAGACCAAG 
CATGGCTCAG TAACGGTTCC AGTGGCTGCA AAGCCTGATG ACACCTCAGA GAGCAGCGAC 
TCATCAGACA GTGAAAATGA GGAGCCTCCA TCAGTAACCC AGTCAAAGCC ATCTTTAAAA 
ACCTCCCAGG CCATCCTATC CCCAGTGAAA ACCACACCAG CAGCCTCAAT CTCCAGCAAA 
GCAGCTCCAG CAAAAACACT TCCACTGTCC CAAGGGAAGC CAACGCCGAA ACCGGCGGTG 
CTAAAGCAGG CTAAAGCCAC ACCAGGAAGG ACTGCTGCTC CTGCAAAGCC AGCTGAAAGT 
GCAAACCCAG CGGAGAGCAC TGACTCTTCA GGCAGTGAAG AGGATCTGCC TGTGCCTGTG 
AGCCAGAAGA GGCTACCAGA ACAGCCTGGG CCCGTTCCCA GCCTGAACCA GGCTGCTAAA 
GCCATGACTC CTGAAAAGGC AGTGGGAAGC ACCTTGAAGA ATGAGACCTC CGAAAGTGGA 
AGCAGTGACT CGTCTGACAG TGAGGAGGAG ACTCCAGTGT CACAGACACA GCCTGCACTG 
CCTGCTGCGA AAAGCAATGC TGTGCCCCAG CCAACAAGCG TGAAGAAAGT AGCGGCTCCC 
ACACCAGCCC CAGCCCCTCC TCTGCATATG GACAGCAGTG ATGACTCCAG TGAGGAGTCG 
GATTCTGATG AGGAAATAGT CCCCCCCACA CAGACTCTAT CCCAGCAGAA CATCAAACCA 
ACCAAGGTTT CAAGCACAGG GGCCGCCAAG GCTAATGCCA CACCACCTTC AGGGAAGGGA 
ATAAAAGCAC CTCCTATCCC TCCGATTTCC AGAGTGTCTG AGAGCTCAAA CAGTGATTCC 
TCAGAGAGCA GTACAGTGGT TGGAGAGGCT GTTCCTCCTC CTTGCCCAAA GCAGACAGCT 
CCCATGGGGA AGGCTCCAGC CAGCCCTGCT GCCCCGCATG CTGCTTCCAT CCTGAGAAGC 
CCCACTGCCA AGTCGAAGAA GCCAGGCCAG CAGCCAGCAC AGGATGCCAG GCTGAGCCAG 
ACTGCACCGC TCACCCAAGC AGAGGAGAGC TCGGACAGCA GCAGTTCCTC TGACAGCGAC 
GAGGAGACAT CCAAGCAGCC TCCCAAACCT GGGATGCTGC AGAAACCAGG AGGGACTGAG 
GCAAATGACA GCTCCTCATC GGATTCCAGT GAGGACGAAG AGGCAGTATC ACAGTCCCTC 
CTCACAGGTT ATATGAGCCT CTCCAAGAGC CCAGCAGCAC CTCAGACACC AAAGACTGTT 
CCCCCCCAGC CTGTAGGCAA TGCAGGGCAA GGGAAAGCAG CTGTGAATGC CACTAGCTCC 
CTCGCCAAAG CCTCCGTGAA AGCAGCCCCA GCTGACAGCT CCAGCAGTGA CAGCAGTGAC 
TCTGACACAG ACGTCGAGCA GGTGTCTGCA AACCACAAAG CAGAAAGCAA ACCCCCTCCG 
GACGAGGACG CCACAGGAGC ATCCAAAAAA GAGAAGGGGG CAGGAAAAAT TGATGTAAGT 
CATGCCAACG CCAAGCCTTC GCCAGTCAAG AAAGCCCTGG CTGCAAAAGA GAATGATGTT 
GAGGCAAAAG AGCTGCAAGC GAACCCATTG CCATCCGCAC TGCTTTCAAC CCCACAGCCA 
GAGGAGTCAA GTCTGGGGAG CAAAAGTGAG GTCACCACTG CTGCTGGAGT TCCCGCAGTG 
CAAACACCAG AAGGGTTGGA AGAGAAGAAG AAGAAAAAAA AAGAGAAAAA AGAAAAGAAG 
GAAAAGAAGA AACATTCCTC CACAGCAGAC AAGGCTGTGA AAACATCTAA GGGCAAAGAC 
AAAGAGAACA AAAAACAGAA AGCATCTCAG AAACGGAAAC GGCCAGGAGA GGATGGAGCT 
GTTGGGCAGC CCAAGGAAAA GAAGCGGAAA GGGCAAACTA GTGAAGAAGT GCCCAAGAAG 
AAGAAAAAGA AAGCAGCTGA TGACCCGGAG AAGCTGCCAG GCAGCAAGGA AAAGAAAAAA 
TCAAGTAAAA AGAAAAAGGC AGGAAAAGAA AAGAAGAACA AAAAGATGCC CACAGAAGGG 
GAGTCGACAG CAGATGGCTC TGCTGAAGTC CACAAGAAGA AGAAGAAGAA GAAGAAAACA 
GCTGAGCCCG AAGGATTGTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015149275.1
CDS	61..3084
Translation	MFAAEVSPGRMPHALVPWSCSCKSRRKPGSRTPSKARRRKLSDEEAAIPEKIRVPDPVSSSESSEKEEDEKEKTKTGNATSITPVTNSAVNVESSEDDDSSSEEEMPSGKGAITVTPAVASGKAANSLHSPVKPTASGSKAAVPCAANRTVLSNKQQPSASVVTPAKAGQKLPSPGKPGRDAAAVSQAGQSKAPVMTKAPESSSSSSSSESEEEKETPTVKAPAPKVELKQAAGESESSSEESSDETSSEEELATAAVQAKPAVKAVQVNSTPVKSAPTTSVSTKKSTVKKRAVTLNQTKHGSVTVPVAAKPDDTSESSDSSDSENEEPPSVTQSKPSLKTSQAILSPVKTTPAASISSKAAPAKTLPLSQGKPTPKPAVLKQAKATPGRTAAPAKPAESANPAESTDSSGSEEDLPVPVSQKRLPEQPGPVPSLNQAAKAMTPEKAVGSTLKNETSESGSSDSSDSEEETPVSQTQPALPAAKSNAVPQPTSVKKVAAPTPAPAPPLHMDSSDDSSEESDSDEEIVPPTQTLSQQNIKPTKVSSTGAAKANATPPSGKGIKAPPIPPISRVSESSNSDSSESSTVVGEAVPPPCPKQTAPMGKAPASPAAPHAASILRSPTAKSKKPGQQPAQDARLSQTAPLTQAEESSDSSSSSDSDEETSKQPPKPGMLQKPGGTEANDSSSSDSSEDEEAVSQSLLTGYMSLSKSPAAPQTPKTVPPQPVGNAGQGKAAVNATSSLAKASVKAAPADSSSSDSSDSDTDVEQVSANHKAESKPPPDEDATGASKKEKGAGKIDVSHANAKPSPVKKALAAKENDVEAKELQANPLPSALLSTPQPEESSLGSKSEVTTAAGVPAVQTPEGLEEKKKKKKEKKEKKEKKKHSSTADKAVKTSKGKDKENKKQKASQKRKRPGEDGAVGQPKEKKRKGQTSEEVPKKKKKKAADDPEKLPGSKEKKKSSKKKKAGKEKKNKKMPTEGESTADGSAEVHKKKKKKKKTAEPEGLL

Target ORF information:

RefSeq Version	XM_015293789.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293789.1

ORF Insert Sequence:

ATGTTTGCTG CAGAGGTGTC TCCTGGTAGG ATGCCTCATG CCTTGGTGCC TTGGTCTTGT 
TCCTGCAAGA GCAGGAGAAA GCCTGGATCT AGAACTCCTT CGAAAGCCAG GAGAAGAAAG 
CTGAGTGATG AGGAGGCAGC CATCCCAGAA AAGATCAGAG TTCCTGATCC TGTGAGCAGC 
TCTGAAAGCT CAGAGAAAGA AGAGGATGAG AAAGAGAAGA CGAAAACTGG AAATGCAACC 
AGCATTACCC CGGTCACGAA CTCAGCAGTG AATGTAGAAA GCAGTGAAGA TGATGACTCC 
TCATCAGAAG AAGAAATGCC AAGTGGAAAA GGTGCAATTA CGGTGACACC AGCTGTAGCG 
AGTGGCAAAG CTGCCAACTC CTTGCATTCT CCTGTTAAAC CCACTGCTTC GGGAAGTAAA 
GCAGCGGTTC CCTGTGCTGC AAACAGAACT GTGCTCTCAA ATAAGCAGCA GCCCAGTGCA 
TCAGTTGTGA CCCCAGCCAA AGCTGGGCAG AAACTGCCTA GTCCTGGGAA ACCTGGACGA 
GATGCAGCAG CCGTGAGCCA AGCAGGACAA AGCAAAGCTC CAGTAATGAC CAAAGCACCG 
GAAAGCTCCA GCAGCAGCAG CTCGTCAGAG AGTGAGGAGG AAAAGGAGAC ACCGACTGTG 
AAGGCCCCAG CCCCCAAAGT GGAGCTGAAA CAGGCGGCTG GGGAATCTGA GAGCAGCAGT 
GAGGAATCAA GTGATGAGAC ATCGTCTGAG GAGGAGCTTG CGACCGCAGC AGTCCAGGCA 
AAACCAGCTG TTAAAGCAGT GCAGGTTAAT TCAACACCTG TGAAAAGTGC ACCTACTACT 
TCAGTGTCCA CCAAGAAGAG CACAGTGAAA AAAAGAGCAG TGACACTGAA CCAGACCAAG 
CATGGCTCAG TAACGGTTCC AGTGGCTGCA AAGCCTGATG ACACCTCAGA GAGCAGCGAC 
TCATCAGACA GTGAAAATGA GGAGCCTCCA TCAGTAACCC AGTCAAAGCC ATCTTTAAAA 
ACCTCCCAGG CCATCCTATC CCCAGTGAAA ACCACACCAG CAGCCTCAAT CTCCAGCAAA 
GCAGCTCCAG CAAAAACACT TCCACTGTCC CAAGGGAAGC CAACGCCGAA ACCGGCGGTG 
CTAAAGCAGG CTAAAGCCAC ACCAGGAAGG ACTGCTGCTC CTGCAAAGCC AGCTGAAAGT 
GCAAACCCAG CGGAGAGCAC TGACTCTTCA GGCAGTGAAG AGGATCTGCC TGTGCCTGTG 
AGCCAGAAGA GGCTACCAGA ACAGCCTGGG CCCGTTCCCA GCCTGAACCA GGCTGCTAAA 
GCCATGACTC CTGAAAAGGC AGTGGGAAGC ACCTTGAAGA ATGAGACCTC CGAAAGTGGA 
AGCAGTGACT CGTCTGACAG TGAGGAGGAG ACTCCAGTGT CACAGACACA GCCTGCACTG 
CCTGCTGCGA AAAGCAATGC TGTGCCCCAG CCAACAAGCG TGAAGAAAGT AGCGGCTCCC 
ACACCAGCCC CAGCCCCTCC TCTGCATATG GACAGCAGTG ATGACTCCAG TGAGGAGTCG 
GATTCTGATG AGGAAATAGT CCCCCCCACA CAGACTCTAT CCCAGCAGAA CATCAAACCA 
ACCAAGGTTT CAAGCACAGG GGCCGCCAAG GCTAATGCCA CACCACCTTC AGGGAAGGGA 
ATAAAAGCAC CTCCTATCCC TCCGATTTCC AGAGTGTCTG AGAGCTCAAA CAGTGATTCC 
TCAGAGAGCA GTACAGTGGT TGGAGAGGCT GTTCCTCCTC CTTGCCCAAA GCAGACAGCT 
CCCATGGGGA AGGCTCCAGC CAGCCCTGCT GCCCCGCATG CTGCTTCCAT CCTGAGAAGC 
CCCACTGCCA AGTCGAAGAA GCCAGGCCAG CAGCCAGCAC AGGATGCCAG GCTGAGCCAG 
ACTGCACCGC TCACCCAAGC AGAGGAGAGC TCGGACAGCA GCAGTTCCTC TGACAGCGAC 
GAGGAGACAT CCAAGCAGCC TCCCAAACCT GGGATGCTGC AGAAACCAGG AGGGACTGAG 
GCAAATGACA GCTCCTCATC GGATTCCAGT GAGGACGAAG AGGCAGTATC ACAGTCCCTC 
CTCACAGGTT ATATGAGCCT CTCCAAGAGC CCAGCAGCAC CTCAGACACC AAAGACTGTT 
CCCCCCCAGC CTGTAGGCAA TGCAGGGCAA GGGAAAGCAG CTGTGAATGC CACTAGCTCC 
CTCGCCAAAG CCTCCGTGAA AGCAGCCCCA GCTGACAGCT CCAGCAGTGA CAGCAGTGAC 
TCTGACACAG ACGTCGAGCA GGTGTCTGCA AACCACAAAG CAGAAAGCAA ACCCCCTCCG 
GACGAGGACG CCACAGGAGC ATCCAAAAAA GAGAAGGGGG CAGGAAAAAT TGATGTAAGT 
CATGCCAACG CCAAGCCTTC GCCAGTCAAG AAAGCCCTGG CTGCAAAAGA GAATGATGTT 
GAGGCAAAAG AGCTGCAAGC GAACCCATTG CCATCCGCAC TGCTTTCAAC CCCACAGCCA 
GAGGAGTCAA GTCTGGGGAG CAAAAGTGAG GTCACCACTG CTGCTGGAGT TCCCGCAGTG 
CAAACACCAG AAGGGTTGGA AGAGAAGAAG AAGAAAAAAA AAGAGAAAAA AGAAAAGAAG 
GAAAAGAAGA AACATTCCTC CACAGCAGAC AAGGCTGTGA AAACATCTAA GGGCAAAGAC 
AAAGAGAACA AAAAACAGAA AGCATCTCAG AAACGGAAAC GGCCAGGAGA GGATGGAGCT 
GTTGGGCAGC CCAAGGAAAA GAAGCGGAAA GGGCAAACTA GTGAAGAAGT GCCCAAGAAG 
AAGAAAAAGA AAGCAGCTGA TGACCCGGAG AAGCTGCCAG GCAGCAAGGA AAAGAAAAAA 
TCAAGTAAAA AGAAAAAGGC AGGAAAAGAA AAGAAGAACA AAAAGATGCC CACAGAAGGG 
GAGTCGACAG CAGATGGCTC TGCTGAAGTC CACAAGAAGA AGAAGAAGAA GAAGAAAACA 
GCTGAGCCCG AAGGATTGTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_015293789.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015149275.1 Gallus gallus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa41928
Clone ID Related Accession (Same CDS sequence)	XM_015293789.1 , XM_015293789.2
Accession Version	XM_015293789.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	treacle protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006100.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015293789.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCTG CAGAGGTGTC TCCTGGTAGG ATGCCTCATG CCTTGGTGCC TTGGTCTTGT 
TCCTGCAAGA GCAGGAGAAA GCCTGGATCT AGAACTCCTT CGAAAGCCAG GAGAAGAAAG 
CTGAGTGATG AGGAGGCAGC CATCCCAGAA AAGATCAGAG TTCCTGATCC TGTGAGCAGC 
TCTGAAAGCT CAGAGAAAGA AGAGGATGAG AAAGAGAAGA CGAAAACTGG AAATGCAACC 
AGCATTACCC CGGTCACGAA CTCAGCAGTG AATGTAGAAA GCAGTGAAGA TGATGACTCC 
TCATCAGAAG AAGAAATGCC AAGTGGAAAA GGTGCAATTA CGGTGACACC AGCTGTAGCG 
AGTGGCAAAG CTGCCAACTC CTTGCATTCT CCTGTTAAAC CCACTGCTTC GGGAAGTAAA 
GCAGCGGTTC CCTGTGCTGC AAACAGAACT GTGCTCTCAA ATAAGCAGCA GCCCAGTGCA 
TCAGTTGTGA CCCCAGCCAA AGCTGGGCAG AAACTGCCTA GTCCTGGGAA ACCTGGACGA 
GATGCAGCAG CCGTGAGCCA AGCAGGACAA AGCAAAGCTC CAGTAATGAC CAAAGCACCG 
GAAAGCTCCA GCAGCAGCAG CTCGTCAGAG AGTGAGGAGG AAAAGGAGAC ACCGACTGTG 
AAGGCCCCAG CCCCCAAAGT GGAGCTGAAA CAGGCGGCTG GGGAATCTGA GAGCAGCAGT 
GAGGAATCAA GTGATGAGAC ATCGTCTGAG GAGGAGCTTG CGACCGCAGC AGTCCAGGCA 
AAACCAGCTG TTAAAGCAGT GCAGGTTAAT TCAACACCTG TGAAAAGTGC ACCTACTACT 
TCAGTGTCCA CCAAGAAGAG CACAGTGAAA AAAAGAGCAG TGACACTGAA CCAGACCAAG 
CATGGCTCAG TAACGGTTCC AGTGGCTGCA AAGCCTGATG ACACCTCAGA GAGCAGCGAC 
TCATCAGACA GTGAAAATGA GGAGCCTCCA TCAGTAACCC AGTCAAAGCC ATCTTTAAAA 
ACCTCCCAGG CCATCCTATC CCCAGTGAAA ACCACACCAG CAGCCTCAAT CTCCAGCAAA 
GCAGCTCCAG CAAAAACACT TCCACTGTCC CAAGGGAAGC CAACGCCGAA ACCGGCGGTG 
CTAAAGCAGG CTAAAGCCAC ACCAGGAAGG ACTGCTGCTC CTGCAAAGCC AGCTGAAAGT 
GCAAACCCAG CGGAGAGCAC TGACTCTTCA GGCAGTGAAG AGGATCTGCC TGTGCCTGTG 
AGCCAGAAGA GGCTACCAGA ACAGCCTGGG CCCGTTCCCA GCCTGAACCA GGCTGCTAAA 
GCCATGACTC CTGAAAAGGC AGTGGGAAGC ACCTTGAAGA ATGAGACCTC CGAAAGTGGA 
AGCAGTGACT CGTCTGACAG TGAGGAGGAG ACTCCAGTGT CACAGACACA GCCTGCACTG 
CCTGCTGCGA AAAGCAATGC TGTGCCCCAG CCAACAAGCG TGAAGAAAGT AGCGGCTCCC 
ACACCAGCCC CAGCCCCTCC TCTGCATATG GACAGCAGTG ATGACTCCAG TGAGGAGTCG 
GATTCTGATG AGGAAATAGT CCCCCCCACA CAGACTCTAT CCCAGCAGAA CATCAAACCA 
ACCAAGGTTT CAAGCACAGG GGCCGCCAAG GCTAATGCCA CACCACCTTC AGGGAAGGGA 
ATAAAAGCAC CTCCTATCCC TCCGATTTCC AGAGTGTCTG AGAGCTCAAA CAGTGATTCC 
TCAGAGAGCA GTACAGTGGT TGGAGAGGCT GTTCCTCCTC CTTGCCCAAA GCAGACAGCT 
CCCATGGGGA AGGCTCCAGC CAGCCCTGCT GCCCCGCATG CTGCTTCCAT CCTGAGAAGC 
CCCACTGCCA AGTCGAAGAA GCCAGGCCAG CAGCCAGCAC AGGATGCCAG GCTGAGCCAG 
ACTGCACCGC TCACCCAAGC AGAGGAGAGC TCGGACAGCA GCAGTTCCTC TGACAGCGAC 
GAGGAGACAT CCAAGCAGCC TCCCAAACCT GGGATGCTGC AGAAACCAGG AGGGACTGAG 
GCAAATGACA GCTCCTCATC GGATTCCAGT GAGGACGAAG AGGCAGTATC ACAGTCCCTC 
CTCACAGGTT ATATGAGCCT CTCCAAGAGC CCAGCAGCAC CTCAGACACC AAAGACTGTT 
CCCCCCCAGC CTGTAGGCAA TGCAGGGCAA GGGAAAGCAG CTGTGAATGC CACTAGCTCC 
CTCGCCAAAG CCTCCGTGAA AGCAGCCCCA GCTGACAGCT CCAGCAGTGA CAGCAGTGAC 
TCTGACACAG ACGTCGAGCA GGTGTCTGCA AACCACAAAG CAGAAAGCAA ACCCCCTCCG 
GACGAGGACG CCACAGGAGC ATCCAAAAAA GAGAAGGGGG CAGGAAAAAT TGATGTAAGT 
CATGCCAACG CCAAGCCTTC GCCAGTCAAG AAAGCCCTGG CTGCAAAAGA GAATGATGTT 
GAGGCAAAAG AGCTGCAAGC GAACCCATTG CCATCCGCAC TGCTTTCAAC CCCACAGCCA 
GAGGAGTCAA GTCTGGGGAG CAAAAGTGAG GTCACCACTG CTGCTGGAGT TCCCGCAGTG 
CAAACACCAG AAGGGTTGGA AGAGAAGAAG AAGAAAAAAA AAGAGAAAAA AGAAAAGAAG 
GAAAAGAAGA AACATTCCTC CACAGCAGAC AAGGCTGTGA AAACATCTAA GGGCAAAGAC 
AAAGAGAACA AAAAACAGAA AGCATCTCAG AAACGGAAAC GGCCAGGAGA GGATGGAGCT 
GTTGGGCAGC CCAAGGAAAA GAAGCGGAAA GGGCAAACTA GTGAAGAAGT GCCCAAGAAG 
AAGAAAAAGA AAGCAGCTGA TGACCCGGAG AAGCTGCCAG GCAGCAAGGA AAAGAAAAAA 
TCAAGTAAAA AGAAAAAGGC AGGAAAAGAA AAGAAGAACA AAAAGATGCC CACAGAAGGG 
GAGTCGACAG CAGATGGCTC TGCTGAAGTC CACAAGAAGA AGAAGAAGAA GAAGAAAACA 
GCTGAGCCCG AAGGATTGTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015149275.1
CDS	57..3080
Translation	MFAAEVSPGRMPHALVPWSCSCKSRRKPGSRTPSKARRRKLSDEEAAIPEKIRVPDPVSSSESSEKEEDEKEKTKTGNATSITPVTNSAVNVESSEDDDSSSEEEMPSGKGAITVTPAVASGKAANSLHSPVKPTASGSKAAVPCAANRTVLSNKQQPSASVVTPAKAGQKLPSPGKPGRDAAAVSQAGQSKAPVMTKAPESSSSSSSSESEEEKETPTVKAPAPKVELKQAAGESESSSEESSDETSSEEELATAAVQAKPAVKAVQVNSTPVKSAPTTSVSTKKSTVKKRAVTLNQTKHGSVTVPVAAKPDDTSESSDSSDSENEEPPSVTQSKPSLKTSQAILSPVKTTPAASISSKAAPAKTLPLSQGKPTPKPAVLKQAKATPGRTAAPAKPAESANPAESTDSSGSEEDLPVPVSQKRLPEQPGPVPSLNQAAKAMTPEKAVGSTLKNETSESGSSDSSDSEEETPVSQTQPALPAAKSNAVPQPTSVKKVAAPTPAPAPPLHMDSSDDSSEESDSDEEIVPPTQTLSQQNIKPTKVSSTGAAKANATPPSGKGIKAPPIPPISRVSESSNSDSSESSTVVGEAVPPPCPKQTAPMGKAPASPAAPHAASILRSPTAKSKKPGQQPAQDARLSQTAPLTQAEESSDSSSSSDSDEETSKQPPKPGMLQKPGGTEANDSSSSDSSEDEEAVSQSLLTGYMSLSKSPAAPQTPKTVPPQPVGNAGQGKAAVNATSSLAKASVKAAPADSSSSDSSDSDTDVEQVSANHKAESKPPPDEDATGASKKEKGAGKIDVSHANAKPSPVKKALAAKENDVEAKELQANPLPSALLSTPQPEESSLGSKSEVTTAAGVPAVQTPEGLEEKKKKKKEKKEKKEKKKHSSTADKAVKTSKGKDKENKKQKASQKRKRPGEDGAVGQPKEKKRKGQTSEEVPKKKKKKAADDPEKLPGSKEKKKSSKKKKAGKEKKNKKMPTEGESTADGSAEVHKKKKKKKKTAEPEGLL

Target ORF information:

RefSeq Version	XM_015293789.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293789.2

ORF Insert Sequence:

ATGTTTGCTG CAGAGGTGTC TCCTGGTAGG ATGCCTCATG CCTTGGTGCC TTGGTCTTGT 
TCCTGCAAGA GCAGGAGAAA GCCTGGATCT AGAACTCCTT CGAAAGCCAG GAGAAGAAAG 
CTGAGTGATG AGGAGGCAGC CATCCCAGAA AAGATCAGAG TTCCTGATCC TGTGAGCAGC 
TCTGAAAGCT CAGAGAAAGA AGAGGATGAG AAAGAGAAGA CGAAAACTGG AAATGCAACC 
AGCATTACCC CGGTCACGAA CTCAGCAGTG AATGTAGAAA GCAGTGAAGA TGATGACTCC 
TCATCAGAAG AAGAAATGCC AAGTGGAAAA GGTGCAATTA CGGTGACACC AGCTGTAGCG 
AGTGGCAAAG CTGCCAACTC CTTGCATTCT CCTGTTAAAC CCACTGCTTC GGGAAGTAAA 
GCAGCGGTTC CCTGTGCTGC AAACAGAACT GTGCTCTCAA ATAAGCAGCA GCCCAGTGCA 
TCAGTTGTGA CCCCAGCCAA AGCTGGGCAG AAACTGCCTA GTCCTGGGAA ACCTGGACGA 
GATGCAGCAG CCGTGAGCCA AGCAGGACAA AGCAAAGCTC CAGTAATGAC CAAAGCACCG 
GAAAGCTCCA GCAGCAGCAG CTCGTCAGAG AGTGAGGAGG AAAAGGAGAC ACCGACTGTG 
AAGGCCCCAG CCCCCAAAGT GGAGCTGAAA CAGGCGGCTG GGGAATCTGA GAGCAGCAGT 
GAGGAATCAA GTGATGAGAC ATCGTCTGAG GAGGAGCTTG CGACCGCAGC AGTCCAGGCA 
AAACCAGCTG TTAAAGCAGT GCAGGTTAAT TCAACACCTG TGAAAAGTGC ACCTACTACT 
TCAGTGTCCA CCAAGAAGAG CACAGTGAAA AAAAGAGCAG TGACACTGAA CCAGACCAAG 
CATGGCTCAG TAACGGTTCC AGTGGCTGCA AAGCCTGATG ACACCTCAGA GAGCAGCGAC 
TCATCAGACA GTGAAAATGA GGAGCCTCCA TCAGTAACCC AGTCAAAGCC ATCTTTAAAA 
ACCTCCCAGG CCATCCTATC CCCAGTGAAA ACCACACCAG CAGCCTCAAT CTCCAGCAAA 
GCAGCTCCAG CAAAAACACT TCCACTGTCC CAAGGGAAGC CAACGCCGAA ACCGGCGGTG 
CTAAAGCAGG CTAAAGCCAC ACCAGGAAGG ACTGCTGCTC CTGCAAAGCC AGCTGAAAGT 
GCAAACCCAG CGGAGAGCAC TGACTCTTCA GGCAGTGAAG AGGATCTGCC TGTGCCTGTG 
AGCCAGAAGA GGCTACCAGA ACAGCCTGGG CCCGTTCCCA GCCTGAACCA GGCTGCTAAA 
GCCATGACTC CTGAAAAGGC AGTGGGAAGC ACCTTGAAGA ATGAGACCTC CGAAAGTGGA 
AGCAGTGACT CGTCTGACAG TGAGGAGGAG ACTCCAGTGT CACAGACACA GCCTGCACTG 
CCTGCTGCGA AAAGCAATGC TGTGCCCCAG CCAACAAGCG TGAAGAAAGT AGCGGCTCCC 
ACACCAGCCC CAGCCCCTCC TCTGCATATG GACAGCAGTG ATGACTCCAG TGAGGAGTCG 
GATTCTGATG AGGAAATAGT CCCCCCCACA CAGACTCTAT CCCAGCAGAA CATCAAACCA 
ACCAAGGTTT CAAGCACAGG GGCCGCCAAG GCTAATGCCA CACCACCTTC AGGGAAGGGA 
ATAAAAGCAC CTCCTATCCC TCCGATTTCC AGAGTGTCTG AGAGCTCAAA CAGTGATTCC 
TCAGAGAGCA GTACAGTGGT TGGAGAGGCT GTTCCTCCTC CTTGCCCAAA GCAGACAGCT 
CCCATGGGGA AGGCTCCAGC CAGCCCTGCT GCCCCGCATG CTGCTTCCAT CCTGAGAAGC 
CCCACTGCCA AGTCGAAGAA GCCAGGCCAG CAGCCAGCAC AGGATGCCAG GCTGAGCCAG 
ACTGCACCGC TCACCCAAGC AGAGGAGAGC TCGGACAGCA GCAGTTCCTC TGACAGCGAC 
GAGGAGACAT CCAAGCAGCC TCCCAAACCT GGGATGCTGC AGAAACCAGG AGGGACTGAG 
GCAAATGACA GCTCCTCATC GGATTCCAGT GAGGACGAAG AGGCAGTATC ACAGTCCCTC 
CTCACAGGTT ATATGAGCCT CTCCAAGAGC CCAGCAGCAC CTCAGACACC AAAGACTGTT 
CCCCCCCAGC CTGTAGGCAA TGCAGGGCAA GGGAAAGCAG CTGTGAATGC CACTAGCTCC 
CTCGCCAAAG CCTCCGTGAA AGCAGCCCCA GCTGACAGCT CCAGCAGTGA CAGCAGTGAC 
TCTGACACAG ACGTCGAGCA GGTGTCTGCA AACCACAAAG CAGAAAGCAA ACCCCCTCCG 
GACGAGGACG CCACAGGAGC ATCCAAAAAA GAGAAGGGGG CAGGAAAAAT TGATGTAAGT 
CATGCCAACG CCAAGCCTTC GCCAGTCAAG AAAGCCCTGG CTGCAAAAGA GAATGATGTT 
GAGGCAAAAG AGCTGCAAGC GAACCCATTG CCATCCGCAC TGCTTTCAAC CCCACAGCCA 
GAGGAGTCAA GTCTGGGGAG CAAAAGTGAG GTCACCACTG CTGCTGGAGT TCCCGCAGTG 
CAAACACCAG AAGGGTTGGA AGAGAAGAAG AAGAAAAAAA AAGAGAAAAA AGAAAAGAAG 
GAAAAGAAGA AACATTCCTC CACAGCAGAC AAGGCTGTGA AAACATCTAA GGGCAAAGAC 
AAAGAGAACA AAAAACAGAA AGCATCTCAG AAACGGAAAC GGCCAGGAGA GGATGGAGCT 
GTTGGGCAGC CCAAGGAAAA GAAGCGGAAA GGGCAAACTA GTGAAGAAGT GCCCAAGAAG 
AAGAAAAAGA AAGCAGCTGA TGACCCGGAG AAGCTGCCAG GCAGCAAGGA AAAGAAAAAA 
TCAAGTAAAA AGAAAAAGGC AGGAAAAGAA AAGAAGAACA AAAAGATGCC CACAGAAGGG 
GAGTCGACAG CAGATGGCTC TGCTGAAGTC CACAAGAAGA AGAAGAAGAA GAAGAAAACA 
GCTGAGCCCG AAGGATTGTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_015293790.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015149276.1 Gallus gallus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OGa41929
Clone ID Related Accession (Same CDS sequence)	XM_015293790.1
Accession Version	XM_015293790.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	treacle protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006100.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGGGCCGCGG GCGACGGGAG CTGCTGGCCC TCATCCACCA GCACCTCCTG 
CGCGCCGGCT ACGCGCGAGC CGCCCGCGAG CTACAGGCGC AGCTCGGACA GAAGCTGCTC 
CCGTCCCTGG CGACGTCGCT GGAGGAGATC TTCACGCACT GGGAGAAAAC TCCTTCGAAA 
GCCAGGAGAA GAAAGCTGAG TGATGAGGAG GCAGCCATCC CAGAAAAGAT CAGAGTTCCT 
GATCCTGTGA GCAGCTCTGA AAGCTCAGAG AAAGAAGAGG ATGAGAAAGA GAAGACGAAA 
ACTGGAAATG CAACCAGCAT TACCCCGGTC ACGAACTCAG CAGTGAATGT AGAAAGCAGT 
GAAGATGATG ACTCCTCATC AGAAGAAGAA ATGCCAAGTG GAAAAGGTGC AATTACGGTG 
ACACCAGCTG TAGCGAGTGG CAAAGCTGCC AACTCCTTGC ATTCTCCTGT TAAACCCACT 
GCTTCGGGAA GTAAAGCAGC GGTTCCCTGT GCTGCAAACA GAACTGTGCT CTCAAATAAG 
CAGCAGCCCA GTGCATCAGT TGTGACCCCA GCCAAAGCTG GGCAGAAACT GCCTAGTCCT 
GGGAAACCTG GACGAGATGC AGCAGCCGTG AGCCAAGCAG GACAAAGCAA AGCTCCAGTA 
ATGACCAAAG CACCGGAAAG CTCCAGCAGC AGCAGCTCGT CAGAGAGTGA GGAGGAAAAG 
GAGACACCGA CTGTGAAGGC CCCAGCCCCC AAAGTGGAGC TGAAACAGGC GGCTGGGGAA 
TCTGAGAGCA GCAGTGAGGA ATCAAGTGAT GAGACATCGT CTGAGGAGGA GCTTGCGACC 
GCAGCAGTCC AGGCAAAACC AGCTGTTAAA GCAGTGCAGG TTAATTCAAC ACCTGTGAAA 
AGTGCACCTA CTACTTCAGT GTCCACCAAG AAGAGCACAG TGAAAAAAAG AGCAGTGACA 
CTGAACCAGA CCAAGCATGG CTCAGTAACG GTTCCAGTGG CTGCAAAGCC TGATGACACC 
TCAGAGAGCA GCGACTCATC AGACAGTGAA AATGAGGAGC CTCCATCAGT AACCCAGTCA 
AAGCCATCTT TAAAAACCTC CCAGGCCATC CTATCCCCAG TGAAAACCAC ACCAGCAGCC 
TCAATCTCCA GCAAAGCAGC TCCAGCAAAA ACACTTCCAC TGTCCCAAGG GAAGCCAACG 
CCGAAACCGG CGGTGCTAAA GCAGGCTAAA GCCACACCAG GAAGGACTGC TGCTCCTGCA 
AAGCCAGCTG AAAGTGCAAA CCCAGCGGAG AGCACTGACT CTTCAGGCAG TGAAGAGGAT 
CTGCCTGTGC CTGTGAGCCA GAAGAGGCTA CCAGAACAGC CTGGGCCCGT TCCCAGCCTG 
AACCAGGCTG CTAAAGCCAT GACTCCTGAA AAGGCAGTGG GAAGCACCTT GAAGAATGAG 
ACCTCCGAAA GTGGAAGCAG TGACTCGTCT GACAGTGAGG AGGAGACTCC AGTGTCACAG 
ACACAGCCTG CACTGCCTGC TGCGAAAAGC AATGCTGTGC CCCAGCCAAC AAGCGTGAAG 
AAAGTAGCGG CTCCCACACC AGCCCCAGCC CCTCCTCTGC ATATGGACAG CAGTGATGAC 
TCCAGTGAGG AGTCGGATTC TGATGAGGAA ATAGTCCCCC CCACACAGGC TGTTCCTCCT 
CCTTGCCCAA AGCAGACAGC TCCCATGGGG AAGGCTCCAG CCAGCCCTGC TGCCCCGCAT 
GCTGCTTCCA TCCTGAGAAG CCCCACTGCC AAGTCGAAGA AGCCAGGCCA GCAGCCAGCA 
CAGGATGCCA GGCTGAGCCA GACTGCACCG CTCACCCAAG CAGAGGAGAG CTCGGACAGC 
AGCAGTTCCT CTGACAGCGA CGAGGAGACA TCCAAGCAGC CTCCCAAACC TGGGATGCTG 
CAGAAACCAG GAGGGACTGA GGCAAATGAC AGCTCCTCAT CGGATTCCAG TGAGGACGAA 
GAGGCAGTAT CACAGTCCCT CCTCACAGGT TATATGAGCC TCTCCAAGAG CCCAGCAGCA 
CCTCAGACAC CAAAGACTGT TCCCCCCCAG CCTGTAGGCA ATGCAGGGCA AGGGAAAGCA 
GCTGTGAATG CCACTAGCTC CCTCGCCAAA GCCTCCGTGA AAGCAGCCCC AGCTGACAGC 
TCCAGCAGTG ACAGCAGTGA CTCTGACACA GACGTCGAGC AGGTGTCTGC AAACCACAAA 
GCAGAAAGCA AACCCCCTCC GGACGAGGAC GCCACAGGAG CATCCAAAAA AGAGAAGGGG 
GCAGGAAAAA TTGATGTAAG TCATGCCAAC GCCAAGCCTT CGCCAGTCAA GAAAGCCCTG 
GCTGCAAAAG AGAATGATGT TGAGGCAAAA GAGCTGCAAG CGAACCCATT GCCATCCGCA 
CTGCTTTCAA CCCCACAGCC AGAGGAGTCA AGTCTGGGGA GCAAAAGTGA GGTCACCACT 
GCTGCTGGAG TTCCCGCAGT GCAAACACCA GAAGGGTTGG AAGAGAAGAA GAAGAAAAAA 
AAAGAGAAAA AAGAAAAGAA GGAAAAGAAG AAACATTCCT CCACAGCAGA CAAGGCTGTG 
AAAACATCTA AGGGCAAAGA CAAAGAGAAC AAAAAACAGA AAGCATCTCA GAAACGGAAA 
CGGCCAGGAG AGGATGGAGC TGTTGGGCAG CCCAAGGAAA AGAAGCGGAA AGGGCAAACT 
AGTGAAGAAG TGCCCAAGAA GAAGAAAAAG AAAGCAGCTG ATGACCCGGA GAAGCTGCCA 
GGCAGCAAGG AAAAGAAAAA ATCAAGTAAA AAGAAAAAGG CAGGAAAAGA AAAGAAGAAC 
AAAAAGATGC CCACAGAAGG GGAGTCGACA GCAGATGGCT CTGCTGAAGT CCACAAGAAG 
AAGAAGAAGA AGAAGAAAAC AGCTGAGCCC GAAGGATTGT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015149276.1
CDS	71..2995
Translation	MAAEGRGRRELLALIHQHLLRAGYARAARELQAQLGQKLLPSLATSLEEIFTHWEKTPSKARRRKLSDEEAAIPEKIRVPDPVSSSESSEKEEDEKEKTKTGNATSITPVTNSAVNVESSEDDDSSSEEEMPSGKGAITVTPAVASGKAANSLHSPVKPTASGSKAAVPCAANRTVLSNKQQPSASVVTPAKAGQKLPSPGKPGRDAAAVSQAGQSKAPVMTKAPESSSSSSSSESEEEKETPTVKAPAPKVELKQAAGESESSSEESSDETSSEEELATAAVQAKPAVKAVQVNSTPVKSAPTTSVSTKKSTVKKRAVTLNQTKHGSVTVPVAAKPDDTSESSDSSDSENEEPPSVTQSKPSLKTSQAILSPVKTTPAASISSKAAPAKTLPLSQGKPTPKPAVLKQAKATPGRTAAPAKPAESANPAESTDSSGSEEDLPVPVSQKRLPEQPGPVPSLNQAAKAMTPEKAVGSTLKNETSESGSSDSSDSEEETPVSQTQPALPAAKSNAVPQPTSVKKVAAPTPAPAPPLHMDSSDDSSEESDSDEEIVPPTQAVPPPCPKQTAPMGKAPASPAAPHAASILRSPTAKSKKPGQQPAQDARLSQTAPLTQAEESSDSSSSSDSDEETSKQPPKPGMLQKPGGTEANDSSSSDSSEDEEAVSQSLLTGYMSLSKSPAAPQTPKTVPPQPVGNAGQGKAAVNATSSLAKASVKAAPADSSSSDSSDSDTDVEQVSANHKAESKPPPDEDATGASKKEKGAGKIDVSHANAKPSPVKKALAAKENDVEAKELQANPLPSALLSTPQPEESSLGSKSEVTTAAGVPAVQTPEGLEEKKKKKKEKKEKKEKKKHSSTADKAVKTSKGKDKENKKQKASQKRKRPGEDGAVGQPKEKKRKGQTSEEVPKKKKKKAADDPEKLPGSKEKKKSSKKKKAGKEKKNKKMPTEGESTADGSAEVHKKKKKKKKTAEPEGLL

Target ORF information:

RefSeq Version	XM_015293790.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293790.1

ORF Insert Sequence:

ATGGCGGCGG AGGGCCGCGG GCGACGGGAG CTGCTGGCCC TCATCCACCA GCACCTCCTG 
CGCGCCGGCT ACGCGCGAGC CGCCCGCGAG CTACAGGCGC AGCTCGGACA GAAGCTGCTC 
CCGTCCCTGG CGACGTCGCT GGAGGAGATC TTCACGCACT GGGAGAAAAC TCCTTCGAAA 
GCCAGGAGAA GAAAGCTGAG TGATGAGGAG GCAGCCATCC CAGAAAAGAT CAGAGTTCCT 
GATCCTGTGA GCAGCTCTGA AAGCTCAGAG AAAGAAGAGG ATGAGAAAGA GAAGACGAAA 
ACTGGAAATG CAACCAGCAT TACCCCGGTC ACGAACTCAG CAGTGAATGT AGAAAGCAGT 
GAAGATGATG ACTCCTCATC AGAAGAAGAA ATGCCAAGTG GAAAAGGTGC AATTACGGTG 
ACACCAGCTG TAGCGAGTGG CAAAGCTGCC AACTCCTTGC ATTCTCCTGT TAAACCCACT 
GCTTCGGGAA GTAAAGCAGC GGTTCCCTGT GCTGCAAACA GAACTGTGCT CTCAAATAAG 
CAGCAGCCCA GTGCATCAGT TGTGACCCCA GCCAAAGCTG GGCAGAAACT GCCTAGTCCT 
GGGAAACCTG GACGAGATGC AGCAGCCGTG AGCCAAGCAG GACAAAGCAA AGCTCCAGTA 
ATGACCAAAG CACCGGAAAG CTCCAGCAGC AGCAGCTCGT CAGAGAGTGA GGAGGAAAAG 
GAGACACCGA CTGTGAAGGC CCCAGCCCCC AAAGTGGAGC TGAAACAGGC GGCTGGGGAA 
TCTGAGAGCA GCAGTGAGGA ATCAAGTGAT GAGACATCGT CTGAGGAGGA GCTTGCGACC 
GCAGCAGTCC AGGCAAAACC AGCTGTTAAA GCAGTGCAGG TTAATTCAAC ACCTGTGAAA 
AGTGCACCTA CTACTTCAGT GTCCACCAAG AAGAGCACAG TGAAAAAAAG AGCAGTGACA 
CTGAACCAGA CCAAGCATGG CTCAGTAACG GTTCCAGTGG CTGCAAAGCC TGATGACACC 
TCAGAGAGCA GCGACTCATC AGACAGTGAA AATGAGGAGC CTCCATCAGT AACCCAGTCA 
AAGCCATCTT TAAAAACCTC CCAGGCCATC CTATCCCCAG TGAAAACCAC ACCAGCAGCC 
TCAATCTCCA GCAAAGCAGC TCCAGCAAAA ACACTTCCAC TGTCCCAAGG GAAGCCAACG 
CCGAAACCGG CGGTGCTAAA GCAGGCTAAA GCCACACCAG GAAGGACTGC TGCTCCTGCA 
AAGCCAGCTG AAAGTGCAAA CCCAGCGGAG AGCACTGACT CTTCAGGCAG TGAAGAGGAT 
CTGCCTGTGC CTGTGAGCCA GAAGAGGCTA CCAGAACAGC CTGGGCCCGT TCCCAGCCTG 
AACCAGGCTG CTAAAGCCAT GACTCCTGAA AAGGCAGTGG GAAGCACCTT GAAGAATGAG 
ACCTCCGAAA GTGGAAGCAG TGACTCGTCT GACAGTGAGG AGGAGACTCC AGTGTCACAG 
ACACAGCCTG CACTGCCTGC TGCGAAAAGC AATGCTGTGC CCCAGCCAAC AAGCGTGAAG 
AAAGTAGCGG CTCCCACACC AGCCCCAGCC CCTCCTCTGC ATATGGACAG CAGTGATGAC 
TCCAGTGAGG AGTCGGATTC TGATGAGGAA ATAGTCCCCC CCACACAGGC TGTTCCTCCT 
CCTTGCCCAA AGCAGACAGC TCCCATGGGG AAGGCTCCAG CCAGCCCTGC TGCCCCGCAT 
GCTGCTTCCA TCCTGAGAAG CCCCACTGCC AAGTCGAAGA AGCCAGGCCA GCAGCCAGCA 
CAGGATGCCA GGCTGAGCCA GACTGCACCG CTCACCCAAG CAGAGGAGAG CTCGGACAGC 
AGCAGTTCCT CTGACAGCGA CGAGGAGACA TCCAAGCAGC CTCCCAAACC TGGGATGCTG 
CAGAAACCAG GAGGGACTGA GGCAAATGAC AGCTCCTCAT CGGATTCCAG TGAGGACGAA 
GAGGCAGTAT CACAGTCCCT CCTCACAGGT TATATGAGCC TCTCCAAGAG CCCAGCAGCA 
CCTCAGACAC CAAAGACTGT TCCCCCCCAG CCTGTAGGCA ATGCAGGGCA AGGGAAAGCA 
GCTGTGAATG CCACTAGCTC CCTCGCCAAA GCCTCCGTGA AAGCAGCCCC AGCTGACAGC 
TCCAGCAGTG ACAGCAGTGA CTCTGACACA GACGTCGAGC AGGTGTCTGC AAACCACAAA 
GCAGAAAGCA AACCCCCTCC GGACGAGGAC GCCACAGGAG CATCCAAAAA AGAGAAGGGG 
GCAGGAAAAA TTGATGTAAG TCATGCCAAC GCCAAGCCTT CGCCAGTCAA GAAAGCCCTG 
GCTGCAAAAG AGAATGATGT TGAGGCAAAA GAGCTGCAAG CGAACCCATT GCCATCCGCA 
CTGCTTTCAA CCCCACAGCC AGAGGAGTCA AGTCTGGGGA GCAAAAGTGA GGTCACCACT 
GCTGCTGGAG TTCCCGCAGT GCAAACACCA GAAGGGTTGG AAGAGAAGAA GAAGAAAAAA 
AAAGAGAAAA AAGAAAAGAA GGAAAAGAAG AAACATTCCT CCACAGCAGA CAAGGCTGTG 
AAAACATCTA AGGGCAAAGA CAAAGAGAAC AAAAAACAGA AAGCATCTCA GAAACGGAAA 
CGGCCAGGAG AGGATGGAGC TGTTGGGCAG CCCAAGGAAA AGAAGCGGAA AGGGCAAACT 
AGTGAAGAAG TGCCCAAGAA GAAGAAAAAG AAAGCAGCTG ATGACCCGGA GAAGCTGCCA 
GGCAGCAAGG AAAAGAAAAA ATCAAGTAAA AAGAAAAAGG CAGGAAAAGA AAAGAAGAAC 
AAAAAGATGC CCACAGAAGG GGAGTCGACA GCAGATGGCT CTGCTGAAGT CCACAAGAAG 
AAGAAGAAGA AGAAGAAAAC AGCTGAGCCC GAAGGATTGT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Genome biology6(1)R6(2005)
Caldwell RB,Kierzek AM,Arakawa H,Bezzubov Y,Zaim J,Fiedler P,Kutter S,Blagodatski A,Kostovska D,Koter M,Plachy J,Carninci P,Hayashizaki Y,Buerstedde JM

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TCOF1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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