GREM1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	GREM1
Entrez Gene ID	395826
Full Name	gremlin 1, DAN family BMP antagonist
Synonyms	Drm
General protein information	Preferred Names gremlin 1, DAN family BMP antagonist Names gremlin-1 Down-regulated by v-mos gremlin 1 homolog, cysteine knot superfamily gremlin 1, cysteine knot superfamily, homolog
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025150591.1	XP_025006359.1	gremlin-1 isoform X1
NM_204978.2	NP_990309.1	gremlin-1 precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	GREM1	NP_990309.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007994	XP_555475.3
Pan troglodytes (chimpanzee)	GREM1	XP_523036.2
	GREM1	NP_001028020.1
Homo sapiens (human)	GREM1	NP_037504.1
Danio rerio (zebrafish)	LOC100005728	XP_005173629.1
Xenopus tropicalis (tropical clawed frog)	grem1	NP_001093701.1
Canis lupus familiaris (dog)	GREM1	XP_005638258.1
Bos taurus (cattle)	GREM1	NP_001075919.1
Mus musculus (house mouse)	Grem1	NP_035954.1
Rattus norvegicus (Norway rat)	Grem1	NP_062155.1

Gene Ontology
Bibliography

Related articles in PubMed

Autopodial development is selectively impaired by misexpression of chordin-like 1 in the chick limb.
Allen JM, McGlinn E, Hill A, Warman ML
Developmental biology381(1)159-69(2013 Sep)

Twist2 contributes to termination of limb bud outgrowth and patterning through direct regulation of Grem1.
Wade C, Brinas I, Welfare M, Wicking C, Farlie PG
Developmental biology370(1)145-53(2012 Oct)

Tgfbeta2 and 3 are coexpressed with their extracellular regulator Ltbp1 in the early limb bud and modulate mesodermal outgrowth and BMP signaling in chicken embryos.
Lorda-Diez CI, Montero JA, Garcia-Porrero JA, Hurle JM
BMC developmental biology1069(2010 Jun)

Regulation of Gremlin expression in the posterior limb bud.
Nissim S, Hasso SM, Fallon JF, Tabin CJ
Developmental biology299(1)12-21(2006 Nov)

Genome duplication in the teleost fish Tetraodon nigroviridis reveals the early vertebrate proto-karyotype.
Jaillon O, Aury JM, Brunet F, Petit JL, Stange-Thomann N, Mauceli E, Bouneau L, Fischer C, Ozouf-Costaz C, Bernot A, Nicaud S, Jaffe D, Fisher S, Lutfalla G, Dossat C, Segurens B, Dasilva C, Salanoubat M, Levy M, Boudet N, Castellano S, Anthouard V, Jubin C, Castelli V, Katinka M, Vacherie B, Bi?mont C, Skalli Z, Cattolico L, Poulain J, De Berardinis V, Cruaud C, Duprat S, Brottier P, Coutanceau JP, Gouzy J, Parra G, Lardier G, Chapple C, McKernan KJ, McEwan P, Bosak S, Kellis M, Volff JN, Guig? R, Zody MC, Mesirov J, Lindblad-Toh K, Birren B, Nusbaum C, Kahn D, Robinson-Rechavi M, Laudet V, Schachter V, Qu?tier F, Saurin W, Scarpelli C, Wincker P, Lander ES, Weissenbach J, Roest Crollius H
Nature431(7011)946-57(2004 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

early stages of the GREM1-SHH-FGF signaling network are resistant to Chrdl1-overexpression, with normal formation of proximal limb structures, but later Bmp expression, impaired by ectopic CHRDL1, is essential for the correct complement of digits
Title: Autopodial development is selectively impaired by misexpression of chordin-like 1 in the chick limb.

Twist2 contributes to termination of limb bud outgrowth and patterning through direct regulation of Grem1.
Title: Twist2 contributes to termination of limb bud outgrowth and patterning through direct regulation of Grem1.

Tgfbeta signaling negatively regulates the expression of the BMP-antagonist Gremlin in the early limb bud.
Title: Tgfbeta2 and 3 are coexpressed with their extracellular regulator Ltbp1 in the early limb bud and modulate mesodermal outgrowth and BMP signaling in chicken embryos.

Bone morphogenetic protein activity is necessary and sufficient for induction of Gremlin.
Title: Regulation of Gremlin expression in the posterior limb bud.

Shh-expressing cells and their descendants cannot express Gremlin; proliferation of these descendants forms a barrier separating the Shh signal from Gremlin-expressing cells which breaks down the Shh-Fgf4 loop and affects limb size
Title: The limb bud Shh-Fgf feedback loop is terminated by expansion of former ZPA cells.

The following GREM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GREM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa28274	NM_204978.2 Latest version!	Gallus gallus gremlin 1, DAN family BMP antagonist (GREM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OGa150774	XM_025150591.1 Latest version!	Gallus gallus gremlin 1, DAN family BMP antagonist (GREM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GREM1 ( NM_204978.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990309.1 Gallus gallus gremlin 1, DAN family BMP antagonist (GREM1), mRNA.

Price & Availability↑

CloneID	OGa28274
Clone ID Related Accession (Same CDS sequence)	NM_204978.2
Accession Version	NM_204978.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Gallus gallus(chicken)
Product	gremlin-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF045799.1. On Aug 30, 2012 this sequence version replaced NM_204978.1. ##Evidence-Data-START## Transcript exon combination :: AF045799.1, CR733243.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTCCGCA CACTGTATGC CATCGGCGCT GTGTTTCTTC TGACGGGATT TCTGCTACCG 
ACAGCAGAAG GGAGAAAGAG GAATCGTGGA TCTCAAGGCG CTATCCCTCC TCCTGACAAG 
GATCAGCCCA ATGATTCAGA GCAGATGCAG ACACAGCAGC AGTCAGGCTC CAGGCATCGA 
GAAAGAGGAA AAGGCACCTC AATGCCTGCT GAGGAGGTGC TGGAGTCCAG CCAGGAGGCA 
CTGCACATCA CTGAGCGCAA ATACCTAAAA CGGGATTGGT GCAAAACTCA GCCCCTCAAA 
CAAACTATCC ATGAAGAAGG CTGCAACAGT CGCACCATTA TCAACAGGTT CTGCTATGGC 
CAATGCAATT CTTTCTACAT CCCCAGGCAT GTCCGCAAAG AGGAAGGCTC TTTCCAGTCT 
TGTTCCTTCT GCAAGCCCAA GAAATTTACC ACCATGACTG TTACCCTCAA CTGCCCTGAG 
CTTCAGCCCC CTAGGAAGAA GAAAAGAATC ACCCGCGTGA AGGAGTGTCG GTGTATATCT 
ATCGACTTGG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990309.1
CDS	80..634
Translation	MVRTLYAIGAVFLLTGFLLPTAEGRKRNRGSQGAIPPPDKDQPNDSEQMQTQQQSGSRHRERGKGTSMPAEEVLESSQEALHITERKYLKRDWCKTQPLKQTIHEEGCNSRTIINRFCYGQCNSFYIPRHVRKEEGSFQSCSFCKPKKFTTMTVTLNCPELQPPRKKKRITRVKECRCISIDLD

Target ORF information:

RefSeq Version	NM_204978.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus gremlin 1, DAN family BMP antagonist (GREM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204978.2

ORF Insert Sequence:

ATGGTCCGCA CACTGTATGC CATCGGCGCT GTGTTTCTTC TGACGGGATT TCTGCTACCG 
ACAGCAGAAG GGAGAAAGAG GAATCGTGGA TCTCAAGGCG CTATCCCTCC TCCTGACAAG 
GATCAGCCCA ATGATTCAGA GCAGATGCAG ACACAGCAGC AGTCAGGCTC CAGGCATCGA 
GAAAGAGGAA AAGGCACCTC AATGCCTGCT GAGGAGGTGC TGGAGTCCAG CCAGGAGGCA 
CTGCACATCA CTGAGCGCAA ATACCTAAAA CGGGATTGGT GCAAAACTCA GCCCCTCAAA 
CAAACTATCC ATGAAGAAGG CTGCAACAGT CGCACCATTA TCAACAGGTT CTGCTATGGC 
CAATGCAATT CTTTCTACAT CCCCAGGCAT GTCCGCAAAG AGGAAGGCTC TTTCCAGTCT 
TGTTCCTTCT GCAAGCCCAA GAAATTTACC ACCATGACTG TTACCCTCAA CTGCCCTGAG 
CTTCAGCCCC CTAGGAAGAA GAAAAGAATC ACCCGCGTGA AGGAGTGTCG GTGTATATCT 
ATCGACTTGG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREM1 ( XM_025150591.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025006359.1 Gallus gallus gremlin 1, DAN family BMP antagonist (GREM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa150774
Clone ID Related Accession (Same CDS sequence)	XM_025150591.1
Accession Version	XM_025150591.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	gremlin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCA CACTGTATGC CATCGGCGCT GTGTTTCTTC TGACGGGATT TCTGCTACCG 
ACAGCAGAAG GGAGAAAGAG GAATCGTGGA TCTCAAGGCG CTATCCCTCC TCCTGACAAG 
GATCAGCCCA ATGATTCAGA GCAGATGCAG ACACAGCAGC AGTCAGGCTC CAGGCATCGA 
GAAAGAGGAA AAGGCACCTC AATGCCTGCT GAGGAGGTGC TGGAGTCCAG CCAGGAGGCA 
CTGCACATCA CTGAGCGCAA ATACCTAAAA CGGGATTGGT GCAAAACTCA GCCCCTCAAA 
CAAACTATCC ATGAAGAAGG CTGCAACAGT CGCACCATTA TCAACAGGTT CTGCTACGGC 
CAATGCAATT CTTTCTACAT CCCCAGGCAT GTCCGCAAAG AGGAAGGCTC TTTCCAGTCT 
TGTTCCTTCT GCAAGCCCAA GAAATTTACC ACCATGACTG TTACCCTCAA CTGCCCTGAG 
CTTCAGCCCC CTAGGAAGAA GAAAAGAATC ACCCGCGTGA AGGAGTGTCG GTGTATATCT 
ATCGACTTGG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025006359.1
CDS	596..1150
Translation	MVRTLYAIGAVFLLTGFLLPTAEGRKRNRGSQGAIPPPDKDQPNDSEQMQTQQQSGSRHRERGKGTSMPAEEVLESSQEALHITERKYLKRDWCKTQPLKQTIHEEGCNSRTIINRFCYGQCNSFYIPRHVRKEEGSFQSCSFCKPKKFTTMTVTLNCPELQPPRKKKRITRVKECRCISIDLD

Target ORF information:

RefSeq Version	XM_025150591.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus gremlin 1, DAN family BMP antagonist (GREM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025150591.1

ORF Insert Sequence:

ATGGTCCGCA CACTGTATGC CATCGGCGCT GTGTTTCTTC TGACGGGATT TCTGCTACCG 
ACAGCAGAAG GGAGAAAGAG GAATCGTGGA TCTCAAGGCG CTATCCCTCC TCCTGACAAG 
GATCAGCCCA ATGATTCAGA GCAGATGCAG ACACAGCAGC AGTCAGGCTC CAGGCATCGA 
GAAAGAGGAA AAGGCACCTC AATGCCTGCT GAGGAGGTGC TGGAGTCCAG CCAGGAGGCA 
CTGCACATCA CTGAGCGCAA ATACCTAAAA CGGGATTGGT GCAAAACTCA GCCCCTCAAA 
CAAACTATCC ATGAAGAAGG CTGCAACAGT CGCACCATTA TCAACAGGTT CTGCTACGGC 
CAATGCAATT CTTTCTACAT CCCCAGGCAT GTCCGCAAAG AGGAAGGCTC TTTCCAGTCT 
TGTTCCTTCT GCAAGCCCAA GAAATTTACC ACCATGACTG TTACCCTCAA CTGCCCTGAG 
CTTCAGCCCC CTAGGAAGAA GAAAAGAATC ACCCGCGTGA AGGAGTGTCG GTGTATATCT 
ATCGACTTGG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Autopodial development is selectively impaired by misexpression of chordin-like 1 in the chick limb.
Developmental biology381(1)159-69(2013 Sep)
Allen JM,McGlinn E,Hill A,Warman ML

Twist2 contributes to termination of limb bud outgrowth and patterning through direct regulation of Grem1.
Developmental biology370(1)145-53(2012 Oct)
Wade C,Brinas I,Welfare M,Wicking C,Farlie PG

Tgfbeta2 and 3 are coexpressed with their extracellular regulator Ltbp1 in the early limb bud and modulate mesodermal outgrowth and BMP signaling in chicken embryos.
BMC developmental biology1069(2010 Jun)
Lorda-Diez CI,Montero JA,Garcia-Porrero JA,Hurle JM

Regulation of Gremlin expression in the posterior limb bud.
Developmental biology299(1)12-21(2006 Nov)
Nissim S,Hasso SM,Fallon JF,Tabin CJ

Genome duplication in the teleost fish Tetraodon nigroviridis reveals the early vertebrate proto-karyotype.
Nature431(7011)946-57(2004 Oct)
Jaillon O,Aury JM,Brunet F,Petit JL,Stange-Thomann N,Mauceli E,Bouneau L,Fischer C,Ozouf-Costaz C,Bernot A,Nicaud S,Jaffe D,Fisher S,Lutfalla G,Dossat C,Segurens B,Dasilva C,Salanoubat M,Levy M,Boudet N,Castellano S,Anthouard V,Jubin C,Castelli V,Katinka M,Vacherie B,Bi?mont C,Skalli Z,Cattolico L,Poulain J,De Berardinis V,Cruaud C,Duprat S,Brottier P,Coutanceau JP,Gouzy J,Parra G,Lardier G,Chapple C,McKernan KJ,McEwan P,Bosak S,Kellis M,Volff JN,Guig? R,Zody MC,Mesirov J,Lindblad-Toh K,Birren B,Nusbaum C,Kahn D,Robinson-Rechavi M,Laudet V,Schachter V,Qu?tier F,Saurin W,Scarpelli C,Wincker P,Lander ES,Weissenbach J,Roest Crollius H

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GREM1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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