PRKDC cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRKDC
Entrez Gene ID	395376
Full Name	protein kinase, DNA-activated, catalytic polypeptide
Synonyms	DNA-PKCS,dnapkcs
General protein information	Preferred Names protein kinase, DNA-activated, catalytic polypeptide Names DNA-dependent protein kinase catalytic subunit DNA-PK catalytic subunit
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_204658.2	NP_989989.2	DNA-dependent protein kinase catalytic subunit

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	prkdc	XP_002934154.2
Gallus gallus (chicken)	PRKDC	NP_989989.1
Danio rerio (zebrafish)	wu:fa96e12	XP_001919588.3
Homo sapiens (human)	PRKDC	NP_008835.5
Canis lupus familiaris (dog)	PRKDC	NP_001006652.1
Pan troglodytes (chimpanzee)	PRKDC	XP_001147162.1
	PRKDC	XP_001100610.2
Bos taurus (cattle)	PRKDC	NP_001243488.1
Mus musculus (house mouse)	Prkdc	NP_035289.2
Rattus norvegicus (Norway rat)	Prkdc	XP_003751148.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003967	XP_318419.5

Gene Ontology
Bibliography

Related articles in PubMed

KU70/80, DNA-PKcs, and Artemis are essential for the rapid induction of apoptosis after massive DSB formation.
Abe T, Ishiai M, Hosono Y, Yoshimura A, Tada S, Adachi N, Koyama H, Takata M, Takeda S, Enomoto T, Seki M
Cellular signalling20(11)1978-85(2008 Nov)

The promoter regions of the Myb-regulated Adora2B and Mcm4 genes co-localize with origins of DNA replication.
Gundelach H, Braas D, Klempnauer KH
BMC molecular biology875(2007 Sep)

Sequence analysis of 193.4 and 83.9 kbp of mouse and chicken genomic DNAs containing the entire Prkdc (DNA-PKcs) gene.
Fujimori A, Hashimoto H, Araki R, Saito T, Sato S, Kasama Y, Tsutsumi Y, Mori M, Fukumura R, Ohhata T, Tatsumi K, Abe M
Radiation research157(3)298-305(2002 Mar)

Identification of four highly conserved regions in DNA-PKcs.
Fujimori A, Araki R, Fukumura R, Ohhata T, Takahashi H, Kawahara A, Tatsumi K, Abe M
Immunogenetics51(11)965-73(2000 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

KU70 and DNA-PKcs unexpectedly function together during the induction of apoptosis.
Title: KU70/80, DNA-PKcs, and Artemis are essential for the rapid induction of apoptosis after massive DSB formation.

The following PRKDC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKDC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa28348	NM_204658.2 Latest version!	Gallus gallus protein kinase, DNA-activated, catalytic polypeptide (PRKDC), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRKDC ( NM_204658.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_989989.2 Gallus gallus protein kinase, DNA-activated, catalytic polypeptide (PRKDC), mRNA.

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CloneID	OGa28348
Clone ID Related Accession (Same CDS sequence)	NM_204658.2
Accession Version	NM_204658.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Gallus gallus(chicken)
Product	DNA-dependent protein kinase catalytic subunit
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB016240.1 and AADN05000031.1. On Jan 4, 2015 this sequence version replaced NM_204658.1. ##Evidence-Data-START## Transcript exon combination :: AB016240.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGGCG GCGTTCAGGA GTGCCTCCAG GAGCTGCACG GCTGCCTGCA GCCCGGCGAT 
GCCATGCGCG GCTACGGGCT GCTGCGCGGC CTGGGCGAGG CCTGCCTCAC CTGCTTGGCC 
GGCGGCGCGC TGGCGCTGCA CGTCTCCCTG GTGTTCGCCC CGGAGCGCGG CCTGCTGGCT 
TTCGTCTGCC GCTCCCTCGG CGTGGAGGAG TTTCGAGAAT GCAGGGAAGA AGCGCTGAAG 
TTTCTGTGCG TCTTTTTGGA AAGGATTGGA GAGAGAGCTC ATCCTTACGC CTGCAGCCTT 
AAGCAAACTT GCATTAGTGT TTATACAAAA GAGAGGGCAG CAAAATGTAA AATTCCAGCT 
CTGGAACTTC TGATTAAGTT GCTCCAAAGT TTACGACGCT CCTGTCTGAT GGAGGAGATG 
AAAGTTGGGG AAATCTTTAA TAAGTTCTAT GGGGAGCTTG CTGTAAGAAG TAAAATTTCT 
GATACAGTAC TGGAGAAGAT CTATGAGCTT TTGGGAGTTT TGGGAGAGGT CCATCCTGCT 
GACATGATTA ACAACTCTGA AAAACTATTC CGAGCGTATC TGGGAGAGCT TAAAACACAG 
ATGACATCAG CAACGAGGGT CCCCAAGCTG CCTATTGTAG CAGGATGTCT GAGGGGACTG 
ACTGCACTCA TGTACAACTT TACCAAGTCT GTGGATGAAG ATGCTCAAAC TTCAAAGGAG 
ATTTTTGATT TTGCAGTGAA GGCTATCAGG CCACAGGTTG ATCAGAAGCG ATATGCAGTA 
CATCTAGCTG GTTTACAGTT GTTCAGCTGG CATGCTGCTC AGTTTGGTAC CCTACTCTTG 
GACAGCTATG TCATGCTCTT TGAAACGATG TGTAGATGGT GTGGCCACAC AAATCAGGAG 
CTGAAGAAAG CCGGTCATAA TGCACTGGAT TCATTCCTCA AGCAGATGTC TTTAATGGTG 
GCAAAGGATG CAGAGCTACA CAAGAGCAAA TTGAAGTTCT TCATGGAGCA GTTCTATGGA 
ATCATCAGAA GAATGGATTC AAGCAACAAG GAGCTGTCCA TTGCTATTCG TGGATATGGG 
CTTTTTGCAG CACCCTGCAA AGCTATGCAT CCTGCAGATG TTGACGCCAT GTACATTGAG 
CTCCTTCAGC GCTGTAAACA AATGTACCTC ACAGAGGCAG AGACAATAGA TGACCATCTG 
TATCAGCTTC CAAGTTTCCT CCAGTCCATC GCAAGTGTGA TATTCCATTT AGACACAATT 
CCAGAAGTAT ACACTCCAGT TCTTGAACAT CTAGTTGTGC TACAAATCAA TAGCTTTCCA 
CGATACAGTG AAAAAATGCA GTTAGTCTGT TGCAGATCTA TAATAAAGGT GTTTCTAGCC 
CTCTCAATAA AAGGTCCTGT TCTCTGGAAT TTTATTAGCA CTGTTGTGCA TCAGGGATTG 
ATCAGAGTAT TCTCCAAGCC AATGAAGTTT TCAAAGGATG TTTTTGGAAA AGAAACTTCT 
GGATCAGAAG AATTCCCAGA ATCTGGAGAG GTTGATACAG CAAGATGGAA AGTACCAACC 
TATAAGGACT ATTTGTATCT CTTCAGAAGT TTACTGAGTT GTGACACGAT GAAGGAATCC 
ATTTTTGAAG AAGAAAACTT TCTTACTGGA AATTCACCAC TGCAGTCTCT GAATCGGTTG 
CTGTATGATG AACTCATAAA ATCTATTTTG AAGATTATTG AGAAGTTGGA CCTGACTGTT 
CAGAAACTGA ATGTCCACGA GCAGGATGAA AATGAAACTG ACAGTGCTTT TATTGGGCCT 
ACTTCTGACC CTGCTTCCAA CCTTCAGCCA AAGAAACCTA CGGACTTTAT TGCATTTATA 
AACCTGGTAG AATTCTGCAG AGACATACTT CCAGATAAAC ATGTTGAATA CTTTCAGCCT 
TGGGTGTATT CCTTTGGTTA TGAGCTTATT ATACACTCAA CGAGACTACC TCTTATCAGT 
GGATTCTACA AGCTGCTTTC TGTTACAATG AAGATTGCCA AGAAAATTAA ATACTTTGAG 
GGTGTCGGTC CAAAGAGTCT CAGAAAAGCT ACTGAAGACC CTGAAAAGAG TTCTTGCTTT 
GCCCTGTTTG CAAAGTTTGG AAAAGAGGTT ACAGCAAAAA TGAAACAGTA TAAAGATGAG 
TTGTTGGCAT CCTGTCTAAA TTTTTTACTC TCTCTACCAC ATGACATCGT CATGCTTGAT 
ATCAAGGCAT ATATTCCTGC ATTGCAGAAT GCGTTTAAGC TGGGCCTTAG CTGTACGCCA 
ATGGCTGATC TGGGACTGGA TGCTTTGGAA GACTGGTCTG CTCACATCCC CAGGCATATA 
ATGCAACCTT ATTACAAGGA TGTGCTACCC CTTCTTGATG GTTATTTGAA AAACTCTGCA 
ACTACAGTTG AACCCCAGAA TAACTGGGAA GTAAGGAAGC TTTCCCGAGC AGCCCAGAAA 
GGATTTAACA AAATCGTGAT ACAGCGTTTA AGAAAAGCAA AAACTTCTTC ATTGGATGAC 
AATCCCTCCT TGGAAGCAGT AAGGACTAGA GTGGCTCGCC TTCTTGGCTC TTTAGGAGGG 
CAGATCAACC ACAACTTAAT TACGGCCACC TCTGCAGAAG AGATGATGAA GAAGTGTGTG 
TCCTGGGACA CCAAGAACCA CCTGAGCTTT GCTGTGCCAT TTGCAGACAT GAAGCCTGTC 
ATCTACTTGG ATGTGTTTCT GCCTCGTGTG ACTGACCTGG CTCTTTCTGC AAGTGACAGA 
CAAACAAAGA TCGCAGCATG TGAACTGCTA CATAGCATTG TTGCATATAT GCTGGGGAAA 
GCATCTCAAA TGCCAGAAAG ACAACAGGGT CCCCCACCCA TGCATCAGTT ATACAAACGA 
ATATTTCCAG TACTGCTTCG TCTTGCCTGT GATGTAGACC AGGTAACGAG ACAATTGTAT 
GAGCCCTTAG TCATGCAGCT CATTCACTGG TTTACCAACA ACAAGAAATT TGAGAGTCAA 
GATACAGTGA CGTTTCTGGA AGCTATCTTG AGTGGCATAG TGGATCCGGT TGACAGCACT 
CTGAGAGATT TCTGCGGTCA GTGTGTACGA GAATTTTTGA AGTGGTCTAT TAAGCAGACA 
ACACCGAAAC AGCAGGAGAA AAGCCCAGCA AACACCAAGT CTCTTTTTAA AAGATTATAC 
AGTCTTGCTC TTCATCCCAG TGCTTTCAAG AGACTAGGTG CAGCACTTGC TTTTAACAGC 
ATCTACAGAG AATTCAGGGA AGAGAATTCT CTGGTGGAGC AGTTTGTGTT TGAAGCATTG 
GTTGTGTTTC TGGAAAGTCT GGCTTTAACC CATACAGACG AGAAATCATT AGGTACCACT 
CAACAATGTT GCGATGCTAT TAATCACCTG AAGCGCATAA TCAAGCATAA AGCACCCGCT 
CTGAACAAGG AAGGAAAACG GCGGGTACCA CGAGGATTTC CAGCTACTAA GTCAGTGTGC 
CTTCAGGATG TTGTCATGTG GCTCTTAGTG CAGTGTGGGC GACCTCAGAC GGAGTGCCGA 
CATAAAGCCA TGGAGCTCTT TTATGAATTT GTTCCTTTGT TACCAGGAAA CAATTCTCCA 
TCTTCTTGGC TGGCTGATGT TTTAAAAAAG AGAGACGTTT CCTTCCTTAT TAACAAATTT 
GAAGGAGGTG GCAGTGATGC CAAAAGCCCG TCTGGGATTC TTTCTCAGCC AACTCTCCGT 
GATATGCAGG AGCCCTTTAG TTTACTGACT GTCATGCGGT GGATGGATAT GTTCTTAGCG 
GCGCTGGACT GCTACAATAC TTTCTTTGAG CTGCGAATGA TCAAGCCTCA TGAAATACTG 
GGTGTAAATG AAAGGTCATC GTTCTTGGAA GCAGTGGATT TCTTCCTCGA AACTATCGCC 
TTGCATGATA TCCATGCAGC AGAGCAGTGC TTTGATTGCA GATCAAGAGG CAACATATTC 
AGTCCACAAG AGAGAGAAGT GTATAATTAC AGTAAGTGTA CAATTATCGT GCGAATCATG 
GAGTTTGTCA CCATGATCCT GGAAATATGC CAGCAGGATT TCTGGAAGCT ACTTGAGAAG 
GAATTGCTTA ATGCAAACTT TATAGAGCTT CTGGTGATGA CAGTGTGTGA TCCGTCACAT 
ATAGGATTTA ATACAGCTGA TGTACAAGTC ATGAAAAATC TCCCAGATAT CTCAGTGAGG 
CTCTTGAAAG CTTTGATGAA ATCTCCCTAT AAAGAGTATT TGCAACTATG TTTAAAAAAA 
AGAATCACCC CACAGAGCTT TGAGGACCTC TGTTCTGTTG ATCTGTTTAA TTCAGATGCA 
CGTTTTGATC AAGTTAGGTT TAGTGCTGTT CTTTCTGCCT GCAAACAACT CCAGAAATCT 
GGTCTGCTTC ATTCTGTTCT TCACAGTCAG GATGAAAGGC CTCATCCCTC AATTGGCTCC 
AAGCTTCTTT CAGTTGTTTA TAAAAGCATT GCACCTGGGA GTGAAAGGAG CTCTCTTCCA 
GCAGTAGACA TCAGCAGTAA GCGATTAGCT GATAGACTCC TCCAGCTTGC GTTTGCCATT 
GATGACCAGT GTGAGGAGCT AGTAAGTCTG CTGTTGAACA CAGTGGTGTT ATCTGTGCCA 
CTATCCAAAG CCTCAGAGAG GAACTTTGTC GATTTTTCTC ATGGACAGTA TTTCTACAGC 
CTGTTCTCAG ACACCATTAA TCAGCAGCTG CTAAAAAATC TGGATGTGAT TGTAATCCAT 
CTTATGGAAT CATCTGTCAG CAATCCCCAA ATGGTTGGAA GCATTTTGAA TGGCATGTTA 
GATCAGAGTT TTAGGGAGCG TACCATCCGC AAGCAGCAAG GAGTGAAACT GGTCACAGCA 
GTACTGAGAA ACTGGAAGAG GCTTGACAGC TGGTGGGCCA AAGATTCATC TCCTGAGAGC 
AAAATGGCAG TCTTGACTCT GCTGGCAAAA GTTCTCCAGA TAGACTCTTC AGTTTCTTTC 
AATACCAGTC ATGAAGCATT TACAGCTGTT TTTGATACAT ATACCAGTCT ACTTACTGAT 
CAGAATTTAG GCTTAAATCT GAAGGGCCAT GCCGTGATCA TTCTTCCATT CTTCACTAAT 
TTGAGTGGAG AAAAACTTCA TGACCTTAAG AATGCTCTTG ATCAACTTGT AGCTTTCAAT 
TTTCCTATGA GTTCAGATGA ATTTCCCAAA GGAACCTTGA AGCACAATAA CTATGTGGAC 
TGCACTAAGA AGTTTTTAGA TGCTTTGGAG TTGTCTCAGA GTCCAATGTT GTTGCAGTTG 
ATGACAGAAG TCCTTTGTAG GGACCACAGA CATTCTATGG AGGACTTATT TCAAGCCAGC 
TTCAAAAGAA TTTCCAGAAG GAGCAGCACT GACAAACAAG TGGTACTGTT GGACACTGTT 
CACAAGATGT TCCAGAATGA AGACCTTCTT TCAAATATTG TCCGTCAAGC TTTTGTGGAT 
CGCTCCCTCC TCACTCTCAT GTCTCACTGC AGCTTGGATG CATTGAGAGA ATTCTTTTGC 
AAGATTATAG TGCAGGCTAT GGATACACTT AATTCCAGGT TTACAAAGTC CAATGAATGT 
GTTTTTGATA CCCAAATCAC CAAGAAAATG GGTTATTACA AGATGCTGGA AGTGATGTAT 
ATACGTCTTT CAAAAGATGA TGTTCACTCC AAGGATTCTA AAATTAACCA AGCTTACCGT 
GGCTCTGTAA GTGTAGAAGG AAATGAACTT ACCAAGGCAC TAATAAAGTT GTGCTATGAT 
GCATTTACAG AAAACATGGC AGGGGAGAAT CAGCTGCTGG AGAAGAGGAG ACAGTATCAC 
TGTGCAGCAT ATAATTGTGC TATTGCTGTT ATTAGTTGTG TCTTCACTGA AAGTAAATTT 
TATCAGGGGT TTCTCTTTAC AGAAAAATCA GAAAAGAACT TGCTGATTTT TGAAAACTTG 
ATAGACTTGA AGCGTCAGTA TGTGTTTCCT GTAGAAATTG AGGTTCCCCT GGAAAGGAAG 
AAGAGATACA TTGCTATTAG AAAAGAAGCC AGAGAGGCTT GGAATGACGG TCAGGATGAA 
CCCAAGTATT TAGCTTCTGC ATCATACATG ATGGATAGCA GTTTGAGTGA GGAAATGAGT 
CAGTTTGATT TTTCTACTGG AGTCCAAGGT TTTTCTTATA GCTCCCAGGA TGTTACAGCA 
AGCAGTGCTC ACTTCAGGAG AAAGGAGACA TCAGAATACA TGGTTTTAAA TGATGAGATG 
GAGCTGGAGA TGGATGAACT TAACCAACAT GAGTGTATGG CACCGTTGAC CACTCTGATT 
AAGCATATGC AGAGGAACCA GATCACACCC AAAGTGGAAG AGGGCGTGAT TCCTGTAGAC 
CTTCCTCTTT GGATGAAATT TCTGCATAGT AAGTTAGGAA ATCCGTCAGT GCCGTTAAAT 
ATTCGCCTCT TCATAGCAAA ACTTATTGTT AATACTGAGG ATGTTTTCCG ACCTTATGCA 
AAGCAATGGC TTGGTCCGAT GCTGCAGCTC GTTGTTTCTG GCGATAATGG AGGTGAAGGG 
ATTCATTATA TGGTGGTGGA AATAGCAGTT ACTGTTCTTT CCTGGACGAG TGTAACTACT 
CCCAAAGGAA ATATTAAGGA TGAGATTTTG GCTAACAGAT TGCTTGAATT CTTGATGAAG 
AATGCCTTTC ATCAGAAACG AGCTGTGTTC AGACACAATC TGGAAATCAT AAAGACAGTT 
ATAGAGTGTT GGAAGAACTG CCTCTCCATA CCTTACAGTT TAATATTTGA AAAGTTTTCT 
AGTGGAGATC CTGATACAAA GGACAACTCA GTGGGAATTC AGCTGCTGGG AATTGTTCTA 
GCTAACAATT TACCTCCCTT TGACCTGAAG TGTGAAATAG ATCGTGTCAG GTATTTTCAG 
GCTCTGGTCA GTAACATGGG CTTATTAAGG TATAAAGAAG TTTATGCAGC TGCAGCAGAA 
GTGTTGGGAC TTGCTCTTCA ATATATAGCT GAGAGACAAA ATATACTTGA AGATCCAGTT 
TATGATTGTG TCATAAAACA ATTAAAACGT CATCAGAACA CCCAACAGGA TAAGTTTATA 
ATATGCTTGA ATAAAGTGGT TAAGAACTTT CCTCCTCTCG CTGACAGGTT TATGAATGCA 
GTATTCTTTT TGATACCAAA ACTTCACGGT GTGATGAAAA ACTACTGTCT GGAAGTAATC 
ATGTGCCGAG CAGAGGAAAT ACCTGATCTC TATTTGCAGC TAAAGAGTAA AGACTTCATC 
CAGATCATGA AACACAGGGA TGATGAAAGG CAAAGGGTTT GTTTGGATAT AATATACAAG 
ATGCTGTCTA CACTGAAACC ACCAGAACTT AAAGAACTTC TTCCTGGAGT GACAGGGTTC 
ATTTCTCACC CTTCTGTAAT ATGTCGACAG CGCATGTATG ATATTCTCAT GTGGATTTAT 
GATAATTACA GTGATCCAGA AAGCCAAGCA GATGGTGATT CTCAGGAAGT GCTCAGTCTG 
GCAAAAGAAA TCCTGCTTCA GGGACTGATT GATGAGAATG CTGAACTGCA ATTAATTGTT 
AGGAATTTTT GGAGTGACGA GACAAGATTA CCTGCAAATA TTCTTGATCG TATGCTGATG 
TTGTTAAATT CTTTATATTC TGCCAAGATT GAAACACAGT ACTTGAGTCT AATCACAAAT 
TTTCTGCTTG AAATGACCAG CAAAAGCCCA GATTATTCCA GAAAAATATT TGAGCACCCA 
CTATCTGAAT GCAAATTTCA TGACTTTGTA ATTGATTCCA GTTGGCGTTA TCGAAGTACC 
ATGCTCACAC CTATGTTTGT GGAGACTCAG GCTTCGCAGA GTACTAACAG GAATTCATCC 
CAAGAAAGAT CCCTTTCCAT TTCTGGGTCA GTAGGTGGTC GAGTGAGAGC TACCCAAAGG 
CAATATGAAT TTACACCTAC ACAGAATGTC AGTGGTAGAA GTTCTTTTAA TTGGCTAACT 
GGAAACAGCA TTGACACTTT GGCAGAGTAT ACAGTTCCTT CTTCCTCCGA GTCTTTGTCT 
TCATCCATGC TGTTAGTTAA CAAAAGGAGT GAAAAATTTA AACAGGCAGC CTTTAAACCT 
GTGGGGCCAG ATTTTGGGAA AAAAAGGCTG AGTTTACCTG GAGATAAGGT GGACAGCAAA 
ACTAAAGGTA TAGATGAACG AGCAGAGATT TTGAGACTGC GGAGGCGTTT CTTAAAGGAT 
CAAGAGAAAG TCAGTCTGAT TTATGCTAGA AAAGGTGTGG CTGAACAGAA AAGAGAAAAG 
GAGATGAAAT CAGAGCTGAA GATGAAGTTT GATGGCCAGG TTACTTTGTA CAGAAGTTAC 
CGTGTAGGAG ACCTTCCTGA CATCCAGATT GAACACTGCA GCCTCATTGC CCCTCTACAG 
GGCCTCGCCC AGAAAGATCC AACATTTGCC AAGCAACTTT TCAGCAGTTT GTTTGGTGGA 
ATTTTTTGTG AGGTGAAAAA ATCTAAAATT CCCTCTGAGA AGAAAGCCAT CATCCAGAAG 
CTGCTGAAGG ACTTCAATCA TTTCCTTAGT ACAAGCGTTT CTTACTTCCC ACCATTCATT 
GCGTGCATCC AGGAGATAAG TTACAAGCAC AGAGAGCTGC TGGAGCTGGA TTCAGCTAAC 
GTGAGCACGA GCTGCCTTGC CAGCCTACAG CAGCCTGTAG GCATCCTTCT TCTGGAGCAT 
GCCCTGATGG CACTGTCCCC TGCAGAGGAG CCCCCATCAA AGCGGATGCG TGGCAGGACT 
GAGCTCCCCC CAGACGTCGT CAAATGGCTC GAGCTTGCTA AGCTTTACCG GTCTCTGGGA 
GATTATGATG TCCTTCGTGG TATTTTCAGT GGTAAGATTG GGACTAAGGA CATTACACAG 
CAAGCGTTGC TGGCAGAAGC AAGGAGTGAT TATGCTGAAG CAGCTAAATG TTATGATGAG 
GCACTCTCCA AAGAAGATTG GGAAGACGGG GAGCCAACTG AAGCAGAGAA AGACTTCTGG 
GAACTTGCAT CTCTGGAATG CTATGACCAC CTCACAGAAT GGAAGTCTCT GGAGTATTGT 
GCTACTGTTA ATATTGACAG TGGTAAACCT CCAGACCTGA ACAAAACGTG GAGTGATCCA 
TTTTATCAGG AGACGTATCT GCCCTATATA ATACGCAGTA AGTTGAAGCT ACTGCTCAAT 
GGGGAAAATG ACCAGACTTT GCTGACTTTC ATTGATGAGG CAATGAAGAC AGAACAGAAG 
AAAGCTCTGA TAGAGATGCA TTACAGCCAA GAGCTTAGTC TCCTTTACAT CCTGCAAGAT 
GATTTTGACA GAGCTAAATA CTACATTGGC AACGGCATAC AGATCTTCAT GCAGAGCTAT 
TCCAGTATAG ATACTTTGTT ATACCAAAGT CGAATGAGCA AACTGCAGTC TGTACAGGCT 
CTGACAGAAA TACAGGATTT CATCAATTTT ATGACCAAAA CAAGTAACAT AGCTTCACAG 
GCTAAATCCC TGAAGAGACT TCTCAGAACT TGGACGAGCA GATATCCAGA TGCTAAAATG 
GATCCCATGA ACATCTGGGA TGATATCATT ACAAATCGGT GCTTCTTTCT TGACAAGCTG 
CAAGAGAAAT TTCTCTGTGA TAAGGCCAAT GATAGTATGG AGGTGGATGA AGAAAGTAGT 
GTTGGAGACC AAATGGAAGT AGATCAACAG GATGAAGACA TTCACTCAAT GATTAGAAGC 
TGCAAATTTA ACATGAAACT GAAGATGATA GAAAGTGCCA GGAAACAGAA CAGCTTCTCC 
ATTGCCAAGA AGCTTCTGAA GGGCCTGCGC AGAGAGGCCA AGACGAGGGA GGACTGGCTG 
GTGAGGTGGA ACTATGCCTA CTGCCGCTTC GTGCACAGCT GCAGCCAGAG CCAGAGTTGT 
CCTGAACGAG TGCTCTCTGT GTTAAAAACC ATCTCCCTGC TTGAAGATAC CAAGTCAGAC 
TACCTTAACA AGAACATAAT GGCCTTCCGA AACCAGAATC TTCTGCTGGG TACTACGTAC 
CACATCATGG CCAATGCTCT TAGTCAGGAT CCAAAATGCT GTGAGCAAAT AGAGGAAGAA 
AAAACTGGAA AAATATTGGA ACTGTCAGGA GAAAGTTCTG AGAGTCCTGA AAAGATATTA 
GCAGGTCTGA ACAAGAGAGC TTTTCAGTGT TTCAGCAGCG CTGCGAGGAA GTCTGAAGAG 
GAAGTGCAGT CCCTCTCCAT GGAACATGTT GATGTGAAGG GAGTTATCGA TGCGTATATG 
ACTTTGGTTG GTTTCTGTGA CCAACACCTG CGAAAGGAAG AAGAAGGCTC ACTTGAAATT 
AATGCTGCAG ATCTGCAGCT ATTCCCAGCT ATTGTGGTGG AGAAAATGAT AAAAGCTTTA 
AAACTGAACT CCAGAGAAGC CAGGCTGAGG TTTCCTCGAC TGCTACAAAT AATTGAAAGA 
TATCCAGCGG AGACGCTGGG TCTGGTGACA CGAGAGCTTC CTTCAGTTCC CTGCTGGCAG 
TTCATCGGTT GGATTAGCCA GATGATGGCA CTCCTTGACA AGGATGAGGC AGTGGCTGTG 
CAGCACACTG TGGAAGAGAT CGTGGATACC TACCCCCAGG CAATCATCTA CCCGTTCACG 
ATCAGCAGTG AGAGTTACTG CTTCAAAGAT ACCGCAGCAG GCTGTAGGAA CAAGGAGTTT 
GTAGCAAGCA TCAAGAACAA GTTGGACAGA GGAGGAGTGG TTCAAGATTT TATTCACGCC 
CTGGAACAGC TCTCTAATCC CGTAATGCTT TTTAAGGACT GGGTTGCGGA TGTCAGAAAT 
GAACTGGTAA AAACTCAGAG AAACAAAATT ATACTTAAGG AAATGTATGA AGGAATGTAT 
AAGAATTTGG GAGATGTGAA GGCTCCCGGC CTTGGGTGGC TGAGGAAACG GTTTGTTCAG 
GCTTTTGGGA AGGATTTTGA CAGTCATTTT GGAAAAGGCG GTTCAAAGCT GTTGGATATG 
AAGATCAGTG ATTTCAATGA GATCACAACA GCTCTTCTCA CCAAAATGAA CAAAACCCAT 
AAAGAACCTG GAAACCTGAA AGAGTGTTCG CCATGGATGA GTGAATTCAG AGCTGAGTTC 
TTGAGGAATG AGCTGGAGGT CCCTGGTCAG TACGATGGGA AGGGGAAACC TCTACCAGAG 
TACCATGTGA AGATCTCTGG ATTTGATGAA CGGATAATGG TATTGGAATC CTTGAGGAAA 
CCCAAGCGCA TAACAATCCG AGGCAGCGAT GAGCAGGAGC ATCCTTTTCT TGTCAAAGGC 
GGAGAAGACC TGCGACAAGA CCAACGCATT GAGCAGCTGT TTGATGTAAT GAACATCGTC 
CTTTCCCGAG ATGCTGCCTG CAGCCAAAGG AATATGCAGT TGAAAACATA CCAGGTCATC 
CCCATGACCA CAAGGCTGGG ACTCATCAAA TGGCTGGAAA ATACCTGTAC CTTGAAAGAA 
TTCCTGAGGA ACAGCATGAC TGAAGAGGAA GATGCCAACT ATAACAGCCT AAAAGGACCT 
CGTGCTGCCT ATTCTGACTG GCTGTCCAAG ATGGGTGGGA AGGCACAAGG CTTATCTCGA 
TACAACGCTA TGTACAAAAA TGCAAGCCGC ACAGAAACGG TTATTGCCTT CAAGAGCAGG 
GAGAGCAGTG TTCCAGAAGA TCTGCTGCGG AGGGCCTTTG TGAAGATGAG CACATCTCCT 
GAAGCTTTCC TGGCTCTGCG GTCGCACTTT GTCAGCTCTC ATGCACTGAT GTGCGTCAGT 
CACTGGATCC TCGGAATTGG AGACAGACAC CTGTCCAACT TCATGATTAA CAAGGAGACG 
GGTGGCATGG TGGGAATAGA CTTTGGACAC GCGTTTGGCT CAGCCACACA GTTTCTGCCC 
GTGCCAGAGC TGATGCCTTT CCGCCTGACG CGCCAGTTTG TCAATCTGAT GATGCCAGTG 
AAGGAGTGGG GCCTCATTTA CAGCGTGATG GTGCATGCCC TGAGAGCGTA CCGTGCGGAT 
CCCGATCTCC TCATCAGCAC AATGGATGTG TTTGTAAAAG AGCCTTCTCT GGACTGGAAG 
AACTTTGAAC AAAGGCAGCT GAAAAAAGGA GGCACGTGGA TTAAGGAAAT AAACACCGCT 
GAAGTAAACT GGTACCCCTT GCAAAAAGTC AGCTATGTCA AAAGGAAGCT TACGGGTGCC 
AATCCTGCAC GAATCACTTG TGATGAGCTG CGCCTGGGCT ATGAGAAGTT GCCTTTTTAC 
AATGACTTTG CAGCTGTTGC TCGTGGCAGC GCTGACCATA ATATCAGAGC CAAAGAGCCA 
GAGGACAGAC TGTCTGAGGA GACCCAGGTG CGGTGCCTGA TAGACCAAGC GACGGACCCC 
AACCTCCTCG GCAGGGTGTG GGAAGGATGG GAACCTTGGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989989.2
CDS	1..12405
Translation	MAGGVQECLQELHGCLQPGDAMRGYGLLRGLGEACLTCLAGGALALHVSLVFAPERGLLAFVCRSLGVEEFRECREEALKFLCVFLERIGERAHPYACSLKQTCISVYTKERAAKCKIPALELLIKLLQSLRRSCLMEEMKVGEIFNKFYGELAVRSKISDTVLEKIYELLGVLGEVHPADMINNSEKLFRAYLGELKTQMTSATRVPKLPIVAGCLRGLTALMYNFTKSVDEDAQTSKEIFDFAVKAIRPQVDQKRYAVHLAGLQLFSWHAAQFGTLLLDSYVMLFETMCRWCGHTNQELKKAGHNALDSFLKQMSLMVAKDAELHKSKLKFFMEQFYGIIRRMDSSNKELSIAIRGYGLFAAPCKAMHPADVDAMYIELLQRCKQMYLTEAETIDDHLYQLPSFLQSIASVIFHLDTIPEVYTPVLEHLVVLQINSFPRYSEKMQLVCCRSIIKVFLALSIKGPVLWNFISTVVHQGLIRVFSKPMKFSKDVFGKETSGSEEFPESGEVDTARWKVPTYKDYLYLFRSLLSCDTMKESIFEEENFLTGNSPLQSLNRLLYDELIKSILKIIEKLDLTVQKLNVHEQDENETDSAFIGPTSDPASNLQPKKPTDFIAFINLVEFCRDILPDKHVEYFQPWVYSFGYELIIHSTRLPLISGFYKLLSVTMKIAKKIKYFEGVGPKSLRKATEDPEKSSCFALFAKFGKEVTAKMKQYKDELLASCLNFLLSLPHDIVMLDIKAYIPALQNAFKLGLSCTPMADLGLDALEDWSAHIPRHIMQPYYKDVLPLLDGYLKNSATTVEPQNNWEVRKLSRAAQKGFNKIVIQRLRKAKTSSLDDNPSLEAVRTRVARLLGSLGGQINHNLITATSAEEMMKKCVSWDTKNHLSFAVPFADMKPVIYLDVFLPRVTDLALSASDRQTKIAACELLHSIVAYMLGKASQMPERQQGPPPMHQLYKRIFPVLLRLACDVDQVTRQLYEPLVMQLIHWFTNNKKFESQDTVTFLEAILSGIVDPVDSTLRDFCGQCVREFLKWSIKQTTPKQQEKSPANTKSLFKRLYSLALHPSAFKRLGAALAFNSIYREFREENSLVEQFVFEALVVFLESLALTHTDEKSLGTTQQCCDAINHLKRIIKHKAPALNKEGKRRVPRGFPATKSVCLQDVVMWLLVQCGRPQTECRHKAMELFYEFVPLLPGNNSPSSWLADVLKKRDVSFLINKFEGGGSDAKSPSGILSQPTLRDMQEPFSLLTVMRWMDMFLAALDCYNTFFELRMIKPHEILGVNERSSFLEAVDFFLETIALHDIHAAEQCFDCRSRGNIFSPQEREVYNYSKCTIIVRIMEFVTMILEICQQDFWKLLEKELLNANFIELLVMTVCDPSHIGFNTADVQVMKNLPDISVRLLKALMKSPYKEYLQLCLKKRITPQSFEDLCSVDLFNSDARFDQVRFSAVLSACKQLQKSGLLHSVLHSQDERPHPSIGSKLLSVVYKSIAPGSERSSLPAVDISSKRLADRLLQLAFAIDDQCEELVSLLLNTVVLSVPLSKASERNFVDFSHGQYFYSLFSDTINQQLLKNLDVIVIHLMESSVSNPQMVGSILNGMLDQSFRERTIRKQQGVKLVTAVLRNWKRLDSWWAKDSSPESKMAVLTLLAKVLQIDSSVSFNTSHEAFTAVFDTYTSLLTDQNLGLNLKGHAVIILPFFTNLSGEKLHDLKNALDQLVAFNFPMSSDEFPKGTLKHNNYVDCTKKFLDALELSQSPMLLQLMTEVLCRDHRHSMEDLFQASFKRISRRSSTDKQVVLLDTVHKMFQNEDLLSNIVRQAFVDRSLLTLMSHCSLDALREFFCKIIVQAMDTLNSRFTKSNECVFDTQITKKMGYYKMLEVMYIRLSKDDVHSKDSKINQAYRGSVSVEGNELTKALIKLCYDAFTENMAGENQLLEKRRQYHCAAYNCAIAVISCVFTESKFYQGFLFTEKSEKNLLIFENLIDLKRQYVFPVEIEVPLERKKRYIAIRKEAREAWNDGQDEPKYLASASYMMDSSLSEEMSQFDFSTGVQGFSYSSQDVTASSAHFRRKETSEYMVLNDEMELEMDELNQHECMAPLTTLIKHMQRNQITPKVEEGVIPVDLPLWMKFLHSKLGNPSVPLNIRLFIAKLIVNTEDVFRPYAKQWLGPMLQLVVSGDNGGEGIHYMVVEIAVTVLSWTSVTTPKGNIKDEILANRLLEFLMKNAFHQKRAVFRHNLEIIKTVIECWKNCLSIPYSLIFEKFSSGDPDTKDNSVGIQLLGIVLANNLPPFDLKCEIDRVRYFQALVSNMGLLRYKEVYAAAAEVLGLALQYIAERQNILEDPVYDCVIKQLKRHQNTQQDKFIICLNKVVKNFPPLADRFMNAVFFLIPKLHGVMKNYCLEVIMCRAEEIPDLYLQLKSKDFIQIMKHRDDERQRVCLDIIYKMLSTLKPPELKELLPGVTGFISHPSVICRQRMYDILMWIYDNYSDPESQADGDSQEVLSLAKEILLQGLIDENAELQLIVRNFWSDETRLPANILDRMLMLLNSLYSAKIETQYLSLITNFLLEMTSKSPDYSRKIFEHPLSECKFHDFVIDSSWRYRSTMLTPMFVETQASQSTNRNSSQERSLSISGSVGGRVRATQRQYEFTPTQNVSGRSSFNWLTGNSIDTLAEYTVPSSSESLSSSMLLVNKRSEKFKQAAFKPVGPDFGKKRLSLPGDKVDSKTKGIDERAEILRLRRRFLKDQEKVSLIYARKGVAEQKREKEMKSELKMKFDGQVTLYRSYRVGDLPDIQIEHCSLIAPLQGLAQKDPTFAKQLFSSLFGGIFCEVKKSKIPSEKKAIIQKLLKDFNHFLSTSVSYFPPFIACIQEISYKHRELLELDSANVSTSCLASLQQPVGILLLEHALMALSPAEEPPSKRMRGRTELPPDVVKWLELAKLYRSLGDYDVLRGIFSGKIGTKDITQQALLAEARSDYAEAAKCYDEALSKEDWEDGEPTEAEKDFWELASLECYDHLTEWKSLEYCATVNIDSGKPPDLNKTWSDPFYQETYLPYIIRSKLKLLLNGENDQTLLTFIDEAMKTEQKKALIEMHYSQELSLLYILQDDFDRAKYYIGNGIQIFMQSYSSIDTLLYQSRMSKLQSVQALTEIQDFINFMTKTSNIASQAKSLKRLLRTWTSRYPDAKMDPMNIWDDIITNRCFFLDKLQEKFLCDKANDSMEVDEESSVGDQMEVDQQDEDIHSMIRSCKFNMKLKMIESARKQNSFSIAKKLLKGLRREAKTREDWLVRWNYAYCRFVHSCSQSQSCPERVLSVLKTISLLEDTKSDYLNKNIMAFRNQNLLLGTTYHIMANALSQDPKCCEQIEEEKTGKILELSGESSESPEKILAGLNKRAFQCFSSAARKSEEEVQSLSMEHVDVKGVIDAYMTLVGFCDQHLRKEEEGSLEINAADLQLFPAIVVEKMIKALKLNSREARLRFPRLLQIIERYPAETLGLVTRELPSVPCWQFIGWISQMMALLDKDEAVAVQHTVEEIVDTYPQAIIYPFTISSESYCFKDTAAGCRNKEFVASIKNKLDRGGVVQDFIHALEQLSNPVMLFKDWVADVRNELVKTQRNKIILKEMYEGMYKNLGDVKAPGLGWLRKRFVQAFGKDFDSHFGKGGSKLLDMKISDFNEITTALLTKMNKTHKEPGNLKECSPWMSEFRAEFLRNELEVPGQYDGKGKPLPEYHVKISGFDERIMVLESLRKPKRITIRGSDEQEHPFLVKGGEDLRQDQRIEQLFDVMNIVLSRDAACSQRNMQLKTYQVIPMTTRLGLIKWLENTCTLKEFLRNSMTEEEDANYNSLKGPRAAYSDWLSKMGGKAQGLSRYNAMYKNASRTETVIAFKSRESSVPEDLLRRAFVKMSTSPEAFLALRSHFVSSHALMCVSHWILGIGDRHLSNFMINKETGGMVGIDFGHAFGSATQFLPVPELMPFRLTRQFVNLMMPVKEWGLIYSVMVHALRAYRADPDLLISTMDVFVKEPSLDWKNFEQRQLKKGGTWIKEINTAEVNWYPLQKVSYVKRKLTGANPARITCDELRLGYEKLPFYNDFAAVARGSADHNIRAKEPEDRLSEETQVRCLIDQATDPNLLGRVWEGWEPWM

Target ORF information:

RefSeq Version	NM_204658.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus protein kinase, DNA-activated, catalytic polypeptide (PRKDC), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204658.2

ORF Insert Sequence:

ATGGCGGGCG GCGTTCAGGA GTGCCTCCAG GAGCTGCACG GCTGCCTGCA GCCCGGCGAT 
GCCATGCGCG GCTACGGGCT GCTGCGCGGC CTGGGCGAGG CCTGCCTCAC CTGCTTGGCC 
GGCGGCGCGC TGGCGCTGCA CGTCTCCCTG GTGTTCGCCC CGGAGCGCGG CCTGCTGGCT 
TTCGTCTGCC GCTCCCTCGG CGTGGAGGAG TTTCGAGAAT GCAGGGAAGA AGCGCTGAAG 
TTTCTGTGCG TCTTTTTGGA AAGGATTGGA GAGAGAGCTC ATCCTTACGC CTGCAGCCTT 
AAGCAAACTT GCATTAGTGT TTATACAAAA GAGAGGGCAG CAAAATGTAA AATTCCAGCT 
CTGGAACTTC TGATTAAGTT GCTCCAAAGT TTACGACGCT CCTGTCTGAT GGAGGAGATG 
AAAGTTGGGG AAATCTTTAA TAAGTTCTAT GGGGAGCTTG CTGTAAGAAG TAAAATTTCT 
GATACAGTAC TGGAGAAGAT CTATGAGCTT TTGGGAGTTT TGGGAGAGGT CCATCCTGCT 
GACATGATTA ACAACTCTGA AAAACTATTC CGAGCGTATC TGGGAGAGCT TAAAACACAG 
ATGACATCAG CAACGAGGGT CCCCAAGCTG CCTATTGTAG CAGGATGTCT GAGGGGACTG 
ACTGCACTCA TGTACAACTT TACCAAGTCT GTGGATGAAG ATGCTCAAAC TTCAAAGGAG 
ATTTTTGATT TTGCAGTGAA GGCTATCAGG CCACAGGTTG ATCAGAAGCG ATATGCAGTA 
CATCTAGCTG GTTTACAGTT GTTCAGCTGG CATGCTGCTC AGTTTGGTAC CCTACTCTTG 
GACAGCTATG TCATGCTCTT TGAAACGATG TGTAGATGGT GTGGCCACAC AAATCAGGAG 
CTGAAGAAAG CCGGTCATAA TGCACTGGAT TCATTCCTCA AGCAGATGTC TTTAATGGTG 
GCAAAGGATG CAGAGCTACA CAAGAGCAAA TTGAAGTTCT TCATGGAGCA GTTCTATGGA 
ATCATCAGAA GAATGGATTC AAGCAACAAG GAGCTGTCCA TTGCTATTCG TGGATATGGG 
CTTTTTGCAG CACCCTGCAA AGCTATGCAT CCTGCAGATG TTGACGCCAT GTACATTGAG 
CTCCTTCAGC GCTGTAAACA AATGTACCTC ACAGAGGCAG AGACAATAGA TGACCATCTG 
TATCAGCTTC CAAGTTTCCT CCAGTCCATC GCAAGTGTGA TATTCCATTT AGACACAATT 
CCAGAAGTAT ACACTCCAGT TCTTGAACAT CTAGTTGTGC TACAAATCAA TAGCTTTCCA 
CGATACAGTG AAAAAATGCA GTTAGTCTGT TGCAGATCTA TAATAAAGGT GTTTCTAGCC 
CTCTCAATAA AAGGTCCTGT TCTCTGGAAT TTTATTAGCA CTGTTGTGCA TCAGGGATTG 
ATCAGAGTAT TCTCCAAGCC AATGAAGTTT TCAAAGGATG TTTTTGGAAA AGAAACTTCT 
GGATCAGAAG AATTCCCAGA ATCTGGAGAG GTTGATACAG CAAGATGGAA AGTACCAACC 
TATAAGGACT ATTTGTATCT CTTCAGAAGT TTACTGAGTT GTGACACGAT GAAGGAATCC 
ATTTTTGAAG AAGAAAACTT TCTTACTGGA AATTCACCAC TGCAGTCTCT GAATCGGTTG 
CTGTATGATG AACTCATAAA ATCTATTTTG AAGATTATTG AGAAGTTGGA CCTGACTGTT 
CAGAAACTGA ATGTCCACGA GCAGGATGAA AATGAAACTG ACAGTGCTTT TATTGGGCCT 
ACTTCTGACC CTGCTTCCAA CCTTCAGCCA AAGAAACCTA CGGACTTTAT TGCATTTATA 
AACCTGGTAG AATTCTGCAG AGACATACTT CCAGATAAAC ATGTTGAATA CTTTCAGCCT 
TGGGTGTATT CCTTTGGTTA TGAGCTTATT ATACACTCAA CGAGACTACC TCTTATCAGT 
GGATTCTACA AGCTGCTTTC TGTTACAATG AAGATTGCCA AGAAAATTAA ATACTTTGAG 
GGTGTCGGTC CAAAGAGTCT CAGAAAAGCT ACTGAAGACC CTGAAAAGAG TTCTTGCTTT 
GCCCTGTTTG CAAAGTTTGG AAAAGAGGTT ACAGCAAAAA TGAAACAGTA TAAAGATGAG 
TTGTTGGCAT CCTGTCTAAA TTTTTTACTC TCTCTACCAC ATGACATCGT CATGCTTGAT 
ATCAAGGCAT ATATTCCTGC ATTGCAGAAT GCGTTTAAGC TGGGCCTTAG CTGTACGCCA 
ATGGCTGATC TGGGACTGGA TGCTTTGGAA GACTGGTCTG CTCACATCCC CAGGCATATA 
ATGCAACCTT ATTACAAGGA TGTGCTACCC CTTCTTGATG GTTATTTGAA AAACTCTGCA 
ACTACAGTTG AACCCCAGAA TAACTGGGAA GTAAGGAAGC TTTCCCGAGC AGCCCAGAAA 
GGATTTAACA AAATCGTGAT ACAGCGTTTA AGAAAAGCAA AAACTTCTTC ATTGGATGAC 
AATCCCTCCT TGGAAGCAGT AAGGACTAGA GTGGCTCGCC TTCTTGGCTC TTTAGGAGGG 
CAGATCAACC ACAACTTAAT TACGGCCACC TCTGCAGAAG AGATGATGAA GAAGTGTGTG 
TCCTGGGACA CCAAGAACCA CCTGAGCTTT GCTGTGCCAT TTGCAGACAT GAAGCCTGTC 
ATCTACTTGG ATGTGTTTCT GCCTCGTGTG ACTGACCTGG CTCTTTCTGC AAGTGACAGA 
CAAACAAAGA TCGCAGCATG TGAACTGCTA CATAGCATTG TTGCATATAT GCTGGGGAAA 
GCATCTCAAA TGCCAGAAAG ACAACAGGGT CCCCCACCCA TGCATCAGTT ATACAAACGA 
ATATTTCCAG TACTGCTTCG TCTTGCCTGT GATGTAGACC AGGTAACGAG ACAATTGTAT 
GAGCCCTTAG TCATGCAGCT CATTCACTGG TTTACCAACA ACAAGAAATT TGAGAGTCAA 
GATACAGTGA CGTTTCTGGA AGCTATCTTG AGTGGCATAG TGGATCCGGT TGACAGCACT 
CTGAGAGATT TCTGCGGTCA GTGTGTACGA GAATTTTTGA AGTGGTCTAT TAAGCAGACA 
ACACCGAAAC AGCAGGAGAA AAGCCCAGCA AACACCAAGT CTCTTTTTAA AAGATTATAC 
AGTCTTGCTC TTCATCCCAG TGCTTTCAAG AGACTAGGTG CAGCACTTGC TTTTAACAGC 
ATCTACAGAG AATTCAGGGA AGAGAATTCT CTGGTGGAGC AGTTTGTGTT TGAAGCATTG 
GTTGTGTTTC TGGAAAGTCT GGCTTTAACC CATACAGACG AGAAATCATT AGGTACCACT 
CAACAATGTT GCGATGCTAT TAATCACCTG AAGCGCATAA TCAAGCATAA AGCACCCGCT 
CTGAACAAGG AAGGAAAACG GCGGGTACCA CGAGGATTTC CAGCTACTAA GTCAGTGTGC 
CTTCAGGATG TTGTCATGTG GCTCTTAGTG CAGTGTGGGC GACCTCAGAC GGAGTGCCGA 
CATAAAGCCA TGGAGCTCTT TTATGAATTT GTTCCTTTGT TACCAGGAAA CAATTCTCCA 
TCTTCTTGGC TGGCTGATGT TTTAAAAAAG AGAGACGTTT CCTTCCTTAT TAACAAATTT 
GAAGGAGGTG GCAGTGATGC CAAAAGCCCG TCTGGGATTC TTTCTCAGCC AACTCTCCGT 
GATATGCAGG AGCCCTTTAG TTTACTGACT GTCATGCGGT GGATGGATAT GTTCTTAGCG 
GCGCTGGACT GCTACAATAC TTTCTTTGAG CTGCGAATGA TCAAGCCTCA TGAAATACTG 
GGTGTAAATG AAAGGTCATC GTTCTTGGAA GCAGTGGATT TCTTCCTCGA AACTATCGCC 
TTGCATGATA TCCATGCAGC AGAGCAGTGC TTTGATTGCA GATCAAGAGG CAACATATTC 
AGTCCACAAG AGAGAGAAGT GTATAATTAC AGTAAGTGTA CAATTATCGT GCGAATCATG 
GAGTTTGTCA CCATGATCCT GGAAATATGC CAGCAGGATT TCTGGAAGCT ACTTGAGAAG 
GAATTGCTTA ATGCAAACTT TATAGAGCTT CTGGTGATGA CAGTGTGTGA TCCGTCACAT 
ATAGGATTTA ATACAGCTGA TGTACAAGTC ATGAAAAATC TCCCAGATAT CTCAGTGAGG 
CTCTTGAAAG CTTTGATGAA ATCTCCCTAT AAAGAGTATT TGCAACTATG TTTAAAAAAA 
AGAATCACCC CACAGAGCTT TGAGGACCTC TGTTCTGTTG ATCTGTTTAA TTCAGATGCA 
CGTTTTGATC AAGTTAGGTT TAGTGCTGTT CTTTCTGCCT GCAAACAACT CCAGAAATCT 
GGTCTGCTTC ATTCTGTTCT TCACAGTCAG GATGAAAGGC CTCATCCCTC AATTGGCTCC 
AAGCTTCTTT CAGTTGTTTA TAAAAGCATT GCACCTGGGA GTGAAAGGAG CTCTCTTCCA 
GCAGTAGACA TCAGCAGTAA GCGATTAGCT GATAGACTCC TCCAGCTTGC GTTTGCCATT 
GATGACCAGT GTGAGGAGCT AGTAAGTCTG CTGTTGAACA CAGTGGTGTT ATCTGTGCCA 
CTATCCAAAG CCTCAGAGAG GAACTTTGTC GATTTTTCTC ATGGACAGTA TTTCTACAGC 
CTGTTCTCAG ACACCATTAA TCAGCAGCTG CTAAAAAATC TGGATGTGAT TGTAATCCAT 
CTTATGGAAT CATCTGTCAG CAATCCCCAA ATGGTTGGAA GCATTTTGAA TGGCATGTTA 
GATCAGAGTT TTAGGGAGCG TACCATCCGC AAGCAGCAAG GAGTGAAACT GGTCACAGCA 
GTACTGAGAA ACTGGAAGAG GCTTGACAGC TGGTGGGCCA AAGATTCATC TCCTGAGAGC 
AAAATGGCAG TCTTGACTCT GCTGGCAAAA GTTCTCCAGA TAGACTCTTC AGTTTCTTTC 
AATACCAGTC ATGAAGCATT TACAGCTGTT TTTGATACAT ATACCAGTCT ACTTACTGAT 
CAGAATTTAG GCTTAAATCT GAAGGGCCAT GCCGTGATCA TTCTTCCATT CTTCACTAAT 
TTGAGTGGAG AAAAACTTCA TGACCTTAAG AATGCTCTTG ATCAACTTGT AGCTTTCAAT 
TTTCCTATGA GTTCAGATGA ATTTCCCAAA GGAACCTTGA AGCACAATAA CTATGTGGAC 
TGCACTAAGA AGTTTTTAGA TGCTTTGGAG TTGTCTCAGA GTCCAATGTT GTTGCAGTTG 
ATGACAGAAG TCCTTTGTAG GGACCACAGA CATTCTATGG AGGACTTATT TCAAGCCAGC 
TTCAAAAGAA TTTCCAGAAG GAGCAGCACT GACAAACAAG TGGTACTGTT GGACACTGTT 
CACAAGATGT TCCAGAATGA AGACCTTCTT TCAAATATTG TCCGTCAAGC TTTTGTGGAT 
CGCTCCCTCC TCACTCTCAT GTCTCACTGC AGCTTGGATG CATTGAGAGA ATTCTTTTGC 
AAGATTATAG TGCAGGCTAT GGATACACTT AATTCCAGGT TTACAAAGTC CAATGAATGT 
GTTTTTGATA CCCAAATCAC CAAGAAAATG GGTTATTACA AGATGCTGGA AGTGATGTAT 
ATACGTCTTT CAAAAGATGA TGTTCACTCC AAGGATTCTA AAATTAACCA AGCTTACCGT 
GGCTCTGTAA GTGTAGAAGG AAATGAACTT ACCAAGGCAC TAATAAAGTT GTGCTATGAT 
GCATTTACAG AAAACATGGC AGGGGAGAAT CAGCTGCTGG AGAAGAGGAG ACAGTATCAC 
TGTGCAGCAT ATAATTGTGC TATTGCTGTT ATTAGTTGTG TCTTCACTGA AAGTAAATTT 
TATCAGGGGT TTCTCTTTAC AGAAAAATCA GAAAAGAACT TGCTGATTTT TGAAAACTTG 
ATAGACTTGA AGCGTCAGTA TGTGTTTCCT GTAGAAATTG AGGTTCCCCT GGAAAGGAAG 
AAGAGATACA TTGCTATTAG AAAAGAAGCC AGAGAGGCTT GGAATGACGG TCAGGATGAA 
CCCAAGTATT TAGCTTCTGC ATCATACATG ATGGATAGCA GTTTGAGTGA GGAAATGAGT 
CAGTTTGATT TTTCTACTGG AGTCCAAGGT TTTTCTTATA GCTCCCAGGA TGTTACAGCA 
AGCAGTGCTC ACTTCAGGAG AAAGGAGACA TCAGAATACA TGGTTTTAAA TGATGAGATG 
GAGCTGGAGA TGGATGAACT TAACCAACAT GAGTGTATGG CACCGTTGAC CACTCTGATT 
AAGCATATGC AGAGGAACCA GATCACACCC AAAGTGGAAG AGGGCGTGAT TCCTGTAGAC 
CTTCCTCTTT GGATGAAATT TCTGCATAGT AAGTTAGGAA ATCCGTCAGT GCCGTTAAAT 
ATTCGCCTCT TCATAGCAAA ACTTATTGTT AATACTGAGG ATGTTTTCCG ACCTTATGCA 
AAGCAATGGC TTGGTCCGAT GCTGCAGCTC GTTGTTTCTG GCGATAATGG AGGTGAAGGG 
ATTCATTATA TGGTGGTGGA AATAGCAGTT ACTGTTCTTT CCTGGACGAG TGTAACTACT 
CCCAAAGGAA ATATTAAGGA TGAGATTTTG GCTAACAGAT TGCTTGAATT CTTGATGAAG 
AATGCCTTTC ATCAGAAACG AGCTGTGTTC AGACACAATC TGGAAATCAT AAAGACAGTT 
ATAGAGTGTT GGAAGAACTG CCTCTCCATA CCTTACAGTT TAATATTTGA AAAGTTTTCT 
AGTGGAGATC CTGATACAAA GGACAACTCA GTGGGAATTC AGCTGCTGGG AATTGTTCTA 
GCTAACAATT TACCTCCCTT TGACCTGAAG TGTGAAATAG ATCGTGTCAG GTATTTTCAG 
GCTCTGGTCA GTAACATGGG CTTATTAAGG TATAAAGAAG TTTATGCAGC TGCAGCAGAA 
GTGTTGGGAC TTGCTCTTCA ATATATAGCT GAGAGACAAA ATATACTTGA AGATCCAGTT 
TATGATTGTG TCATAAAACA ATTAAAACGT CATCAGAACA CCCAACAGGA TAAGTTTATA 
ATATGCTTGA ATAAAGTGGT TAAGAACTTT CCTCCTCTCG CTGACAGGTT TATGAATGCA 
GTATTCTTTT TGATACCAAA ACTTCACGGT GTGATGAAAA ACTACTGTCT GGAAGTAATC 
ATGTGCCGAG CAGAGGAAAT ACCTGATCTC TATTTGCAGC TAAAGAGTAA AGACTTCATC 
CAGATCATGA AACACAGGGA TGATGAAAGG CAAAGGGTTT GTTTGGATAT AATATACAAG 
ATGCTGTCTA CACTGAAACC ACCAGAACTT AAAGAACTTC TTCCTGGAGT GACAGGGTTC 
ATTTCTCACC CTTCTGTAAT ATGTCGACAG CGCATGTATG ATATTCTCAT GTGGATTTAT 
GATAATTACA GTGATCCAGA AAGCCAAGCA GATGGTGATT CTCAGGAAGT GCTCAGTCTG 
GCAAAAGAAA TCCTGCTTCA GGGACTGATT GATGAGAATG CTGAACTGCA ATTAATTGTT 
AGGAATTTTT GGAGTGACGA GACAAGATTA CCTGCAAATA TTCTTGATCG TATGCTGATG 
TTGTTAAATT CTTTATATTC TGCCAAGATT GAAACACAGT ACTTGAGTCT AATCACAAAT 
TTTCTGCTTG AAATGACCAG CAAAAGCCCA GATTATTCCA GAAAAATATT TGAGCACCCA 
CTATCTGAAT GCAAATTTCA TGACTTTGTA ATTGATTCCA GTTGGCGTTA TCGAAGTACC 
ATGCTCACAC CTATGTTTGT GGAGACTCAG GCTTCGCAGA GTACTAACAG GAATTCATCC 
CAAGAAAGAT CCCTTTCCAT TTCTGGGTCA GTAGGTGGTC GAGTGAGAGC TACCCAAAGG 
CAATATGAAT TTACACCTAC ACAGAATGTC AGTGGTAGAA GTTCTTTTAA TTGGCTAACT 
GGAAACAGCA TTGACACTTT GGCAGAGTAT ACAGTTCCTT CTTCCTCCGA GTCTTTGTCT 
TCATCCATGC TGTTAGTTAA CAAAAGGAGT GAAAAATTTA AACAGGCAGC CTTTAAACCT 
GTGGGGCCAG ATTTTGGGAA AAAAAGGCTG AGTTTACCTG GAGATAAGGT GGACAGCAAA 
ACTAAAGGTA TAGATGAACG AGCAGAGATT TTGAGACTGC GGAGGCGTTT CTTAAAGGAT 
CAAGAGAAAG TCAGTCTGAT TTATGCTAGA AAAGGTGTGG CTGAACAGAA AAGAGAAAAG 
GAGATGAAAT CAGAGCTGAA GATGAAGTTT GATGGCCAGG TTACTTTGTA CAGAAGTTAC 
CGTGTAGGAG ACCTTCCTGA CATCCAGATT GAACACTGCA GCCTCATTGC CCCTCTACAG 
GGCCTCGCCC AGAAAGATCC AACATTTGCC AAGCAACTTT TCAGCAGTTT GTTTGGTGGA 
ATTTTTTGTG AGGTGAAAAA ATCTAAAATT CCCTCTGAGA AGAAAGCCAT CATCCAGAAG 
CTGCTGAAGG ACTTCAATCA TTTCCTTAGT ACAAGCGTTT CTTACTTCCC ACCATTCATT 
GCGTGCATCC AGGAGATAAG TTACAAGCAC AGAGAGCTGC TGGAGCTGGA TTCAGCTAAC 
GTGAGCACGA GCTGCCTTGC CAGCCTACAG CAGCCTGTAG GCATCCTTCT TCTGGAGCAT 
GCCCTGATGG CACTGTCCCC TGCAGAGGAG CCCCCATCAA AGCGGATGCG TGGCAGGACT 
GAGCTCCCCC CAGACGTCGT CAAATGGCTC GAGCTTGCTA AGCTTTACCG GTCTCTGGGA 
GATTATGATG TCCTTCGTGG TATTTTCAGT GGTAAGATTG GGACTAAGGA CATTACACAG 
CAAGCGTTGC TGGCAGAAGC AAGGAGTGAT TATGCTGAAG CAGCTAAATG TTATGATGAG 
GCACTCTCCA AAGAAGATTG GGAAGACGGG GAGCCAACTG AAGCAGAGAA AGACTTCTGG 
GAACTTGCAT CTCTGGAATG CTATGACCAC CTCACAGAAT GGAAGTCTCT GGAGTATTGT 
GCTACTGTTA ATATTGACAG TGGTAAACCT CCAGACCTGA ACAAAACGTG GAGTGATCCA 
TTTTATCAGG AGACGTATCT GCCCTATATA ATACGCAGTA AGTTGAAGCT ACTGCTCAAT 
GGGGAAAATG ACCAGACTTT GCTGACTTTC ATTGATGAGG CAATGAAGAC AGAACAGAAG 
AAAGCTCTGA TAGAGATGCA TTACAGCCAA GAGCTTAGTC TCCTTTACAT CCTGCAAGAT 
GATTTTGACA GAGCTAAATA CTACATTGGC AACGGCATAC AGATCTTCAT GCAGAGCTAT 
TCCAGTATAG ATACTTTGTT ATACCAAAGT CGAATGAGCA AACTGCAGTC TGTACAGGCT 
CTGACAGAAA TACAGGATTT CATCAATTTT ATGACCAAAA CAAGTAACAT AGCTTCACAG 
GCTAAATCCC TGAAGAGACT TCTCAGAACT TGGACGAGCA GATATCCAGA TGCTAAAATG 
GATCCCATGA ACATCTGGGA TGATATCATT ACAAATCGGT GCTTCTTTCT TGACAAGCTG 
CAAGAGAAAT TTCTCTGTGA TAAGGCCAAT GATAGTATGG AGGTGGATGA AGAAAGTAGT 
GTTGGAGACC AAATGGAAGT AGATCAACAG GATGAAGACA TTCACTCAAT GATTAGAAGC 
TGCAAATTTA ACATGAAACT GAAGATGATA GAAAGTGCCA GGAAACAGAA CAGCTTCTCC 
ATTGCCAAGA AGCTTCTGAA GGGCCTGCGC AGAGAGGCCA AGACGAGGGA GGACTGGCTG 
GTGAGGTGGA ACTATGCCTA CTGCCGCTTC GTGCACAGCT GCAGCCAGAG CCAGAGTTGT 
CCTGAACGAG TGCTCTCTGT GTTAAAAACC ATCTCCCTGC TTGAAGATAC CAAGTCAGAC 
TACCTTAACA AGAACATAAT GGCCTTCCGA AACCAGAATC TTCTGCTGGG TACTACGTAC 
CACATCATGG CCAATGCTCT TAGTCAGGAT CCAAAATGCT GTGAGCAAAT AGAGGAAGAA 
AAAACTGGAA AAATATTGGA ACTGTCAGGA GAAAGTTCTG AGAGTCCTGA AAAGATATTA 
GCAGGTCTGA ACAAGAGAGC TTTTCAGTGT TTCAGCAGCG CTGCGAGGAA GTCTGAAGAG 
GAAGTGCAGT CCCTCTCCAT GGAACATGTT GATGTGAAGG GAGTTATCGA TGCGTATATG 
ACTTTGGTTG GTTTCTGTGA CCAACACCTG CGAAAGGAAG AAGAAGGCTC ACTTGAAATT 
AATGCTGCAG ATCTGCAGCT ATTCCCAGCT ATTGTGGTGG AGAAAATGAT AAAAGCTTTA 
AAACTGAACT CCAGAGAAGC CAGGCTGAGG TTTCCTCGAC TGCTACAAAT AATTGAAAGA 
TATCCAGCGG AGACGCTGGG TCTGGTGACA CGAGAGCTTC CTTCAGTTCC CTGCTGGCAG 
TTCATCGGTT GGATTAGCCA GATGATGGCA CTCCTTGACA AGGATGAGGC AGTGGCTGTG 
CAGCACACTG TGGAAGAGAT CGTGGATACC TACCCCCAGG CAATCATCTA CCCGTTCACG 
ATCAGCAGTG AGAGTTACTG CTTCAAAGAT ACCGCAGCAG GCTGTAGGAA CAAGGAGTTT 
GTAGCAAGCA TCAAGAACAA GTTGGACAGA GGAGGAGTGG TTCAAGATTT TATTCACGCC 
CTGGAACAGC TCTCTAATCC CGTAATGCTT TTTAAGGACT GGGTTGCGGA TGTCAGAAAT 
GAACTGGTAA AAACTCAGAG AAACAAAATT ATACTTAAGG AAATGTATGA AGGAATGTAT 
AAGAATTTGG GAGATGTGAA GGCTCCCGGC CTTGGGTGGC TGAGGAAACG GTTTGTTCAG 
GCTTTTGGGA AGGATTTTGA CAGTCATTTT GGAAAAGGCG GTTCAAAGCT GTTGGATATG 
AAGATCAGTG ATTTCAATGA GATCACAACA GCTCTTCTCA CCAAAATGAA CAAAACCCAT 
AAAGAACCTG GAAACCTGAA AGAGTGTTCG CCATGGATGA GTGAATTCAG AGCTGAGTTC 
TTGAGGAATG AGCTGGAGGT CCCTGGTCAG TACGATGGGA AGGGGAAACC TCTACCAGAG 
TACCATGTGA AGATCTCTGG ATTTGATGAA CGGATAATGG TATTGGAATC CTTGAGGAAA 
CCCAAGCGCA TAACAATCCG AGGCAGCGAT GAGCAGGAGC ATCCTTTTCT TGTCAAAGGC 
GGAGAAGACC TGCGACAAGA CCAACGCATT GAGCAGCTGT TTGATGTAAT GAACATCGTC 
CTTTCCCGAG ATGCTGCCTG CAGCCAAAGG AATATGCAGT TGAAAACATA CCAGGTCATC 
CCCATGACCA CAAGGCTGGG ACTCATCAAA TGGCTGGAAA ATACCTGTAC CTTGAAAGAA 
TTCCTGAGGA ACAGCATGAC TGAAGAGGAA GATGCCAACT ATAACAGCCT AAAAGGACCT 
CGTGCTGCCT ATTCTGACTG GCTGTCCAAG ATGGGTGGGA AGGCACAAGG CTTATCTCGA 
TACAACGCTA TGTACAAAAA TGCAAGCCGC ACAGAAACGG TTATTGCCTT CAAGAGCAGG 
GAGAGCAGTG TTCCAGAAGA TCTGCTGCGG AGGGCCTTTG TGAAGATGAG CACATCTCCT 
GAAGCTTTCC TGGCTCTGCG GTCGCACTTT GTCAGCTCTC ATGCACTGAT GTGCGTCAGT 
CACTGGATCC TCGGAATTGG AGACAGACAC CTGTCCAACT TCATGATTAA CAAGGAGACG 
GGTGGCATGG TGGGAATAGA CTTTGGACAC GCGTTTGGCT CAGCCACACA GTTTCTGCCC 
GTGCCAGAGC TGATGCCTTT CCGCCTGACG CGCCAGTTTG TCAATCTGAT GATGCCAGTG 
AAGGAGTGGG GCCTCATTTA CAGCGTGATG GTGCATGCCC TGAGAGCGTA CCGTGCGGAT 
CCCGATCTCC TCATCAGCAC AATGGATGTG TTTGTAAAAG AGCCTTCTCT GGACTGGAAG 
AACTTTGAAC AAAGGCAGCT GAAAAAAGGA GGCACGTGGA TTAAGGAAAT AAACACCGCT 
GAAGTAAACT GGTACCCCTT GCAAAAAGTC AGCTATGTCA AAAGGAAGCT TACGGGTGCC 
AATCCTGCAC GAATCACTTG TGATGAGCTG CGCCTGGGCT ATGAGAAGTT GCCTTTTTAC 
AATGACTTTG CAGCTGTTGC TCGTGGCAGC GCTGACCATA ATATCAGAGC CAAAGAGCCA 
GAGGACAGAC TGTCTGAGGA GACCCAGGTG CGGTGCCTGA TAGACCAAGC GACGGACCCC 
AACCTCCTCG GCAGGGTGTG GGAAGGATGG GAACCTTGGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

KU70/80, DNA-PKcs, and Artemis are essential for the rapid induction of apoptosis after massive DSB formation.
Cellular signalling20(11)1978-85(2008 Nov)
Abe T,Ishiai M,Hosono Y,Yoshimura A,Tada S,Adachi N,Koyama H,Takata M,Takeda S,Enomoto T,Seki M

The promoter regions of the Myb-regulated Adora2B and Mcm4 genes co-localize with origins of DNA replication.
BMC molecular biology875(2007 Sep)
Gundelach H,Braas D,Klempnauer KH

Sequence analysis of 193.4 and 83.9 kbp of mouse and chicken genomic DNAs containing the entire Prkdc (DNA-PKcs) gene.
Radiation research157(3)298-305(2002 Mar)
Fujimori A,Hashimoto H,Araki R,Saito T,Sato S,Kasama Y,Tsutsumi Y,Mori M,Fukumura R,Ohhata T,Tatsumi K,Abe M

Identification of four highly conserved regions in DNA-PKcs.
Immunogenetics51(11)965-73(2000 Sep)
Fujimori A,Araki R,Fukumura R,Ohhata T,Takahashi H,Kawahara A,Tatsumi K,Abe M

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PRKDC cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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