ADGRF5 cDNA ORF clone, Gallus gallus(chicken)

The following ADGRF5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADGRF5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa149080 XM_025149284.1
Latest version!
Gallus gallus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa34448 XM_015285168.1
Latest version!
Gallus gallus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa07171 XM_015285166.2
Latest version!
Gallus gallus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OGa07171 XM_015285167.2
Latest version!
Gallus gallus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OGa07171 XM_004935933.3
Latest version!
Gallus gallus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OGa07171 XM_025149283.1
Latest version!
Gallus gallus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OGa07171 XM_004935931.3
Latest version!
Gallus gallus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OGa07171 XM_004935931.2 Gallus gallus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OGa07171 XM_004935933.2 Gallus gallus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OGa07171 XM_015285166.1 Gallus gallus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OGa07171 XM_015285167.1 Gallus gallus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa149080
    Clone ID Related Accession (Same CDS sequence) XM_025149284.1
    Accession Version XM_025149284.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3726bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product adhesion G protein-coupled receptor F5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACAGTTT CAAGTATCAA CATCACAACA GTCTGTGTGC CCAGTGAGAA CAAGAGCTGC 
    TGTTCTTGTG AAAGCGGATA TGCCTGGCCA AGCACGGGGT GCAGTGACCT GATCAGCTGC
    CCTGCTCTCA GCCGAGCTCC CAACCAGCCA TGCGGCTACA CCAGGGAAAG GCCTTTCAAT
    GGGCCCTACT GTGAACCTCA GAATGGAGAC TCATGTGGTA TGGGAGAGCC CACTGTAATG
    AATATGTCAG TAAGACTTGA CACAGCCTTC CACGATGATC TCAGGAATCA TTCTTCCCAA
    CTATACAGAA AATACAAAGC TGACCTCGAT AAAGCGTTTA AAGCTAGCTA CGGGTGCTTA
    CCGGGCTTTG TGTCAGCAAC AACAACTGGT TTCAGGCCTG GGAGTGTTGA GGTGAACTAC
    AGAGTGGAGG CAGGATCAGC GAGCTTCCAA CAGCTGGAGC ATTCCAGCAG ATTTGTTACA
    CAGTACCTGG ATGAACCATA CAGCATAGTG CCTACCTCCT TTACAGCAGA AATAACGAAT
    CGCACAAACT TCACAGTGAG CCCTGCCCAC ATTTTTGAAG GGGATACCAT CCAGATGGTG
    TGTGAGATAA ACACCACGTC TGAGAACATG ACCTGGTATC GTGACAGTCA GATCATCCTC
    AACAGCTCAC GGCACTCCGT GAGGACAGAC AACAACACTG CGATGTCACA GGCATTTCTT
    ACAATTACCA ACGTTACGCA GGAGGATTCT GGTACCTACA AATGCACTTT TGCAAACAGA
    TACCGGTCTT TAAATCTGAT ATACGAAGGC ACCAAAGAAA TACAAGTCTC TCCACTACGT
    ATCATCTCAT ATTCAAACGA CTATAAAGTT CACTGCAACA GCCCTGAGAT ACAGGCAAAC
    AGCCCTGTGC TGTTCTGTTG CATTGATGGA CAATTACCAC TGCTTACTGG TGACTGGAAG
    GTGAATGGAG CGATCAGTAT TACAGGAGTC CCCAGTTTCA GCAGCAACTG CACTGAATAC
    AAGCTCAACA TGACGGACTC GCTGTGCTTG GCCGAGCATT CAGGTACGGT GACGACATAC
    ACGTGTGAGC TGCAGACAGG GCACGGCGCC AGGAGCAGCC GGGACATCCG AGTGATGTAC
    TTCCGAGCAG CCCAGGTAAG AATATCTTCC AGCGTAAACA CAACCGTTTC CGTGGGATAT
    GGCTTCAGTC TAAAGTGTGA AAGCGATACG AGCAATTACG ACAGCATCAG CTGGGAAATC
    CATTCTGGAG ATAGAATACA AGCAGTAGAA TGCGCTGCAT GCATTAGAAC CAACAAATCC
    ATGGCCACAT CCATGCTCGT GGTGAACACT GCCACTCAAG ACTGGAATGG CACCTACATC
    TGCACATTGT CCCAGGACTC CCTGAACAGT TCTGCCAGCG TGACAATCAA TGTCATTCCG
    TTGCCCCTGA AGCAGCACAT CCTGATAGAC CCCGCTGGAG CGCCTATCAT GTGCGGAACG
    CCCCAAGCCC TCACCTGCTG CATCAGTGCT AACAGCATGG GAGATTATGA TGTTAAATTT
    GTGGTACAAG AAAAAGAATT TCAAGCTGAG AAAGAAGAGC AAGGAAATTT CTTTTGCTAC
    TCGTACAATT ACACAGAAAC AGAATGCAGG GAAAAGAAGC TGGAGGCGTA TTGCAAGTTT
    GTGAACAGCA ATGAGCAGGA GGTCAGCAGC AACAGTATAA ATCTGAGCCT GATACCTGAT
    AAGGACCTCA TTTCCTGCTC AGAAAACAAT ACCTTTGGAA AAGAAGGAGA CATATTGAAA
    AAGTCGTGCT CTGTTTTAAA TACTACTTTT ATCCGAGGCA GCAACATTTA CAAGTGCTTC
    AACAGCTCAT GGAAGATGGA AAAGAATGAC TGCGTATCCG AACAGATAAA CAACCTGCTG
    CTCATGGCAG AGTCCTTGGT CAACAGTCCC AGTGCAAAAG TTGGTCTACC TAACTTCCTT
    CAGAACCTGC GGAATCAGAC AGAACTGCTG CAAAATGACA ATTCTTCTGC AAATCTTGCT
    GCAATTGTTA CCATCCTCTA CAACATCTCT TCTATTCCAG TAGAAGCAAA GCAGTCCACT
    ATCTCTGATT ACTTCACTAC AGTGGACATT ATCGTTACTG ATTTCAAAAA GGAACTTTGG
    GCTGACCTGA ATGAAGGGCA GGTACCCATA AGTTCTTTGT TACTGAATGC GGTGGAAAGA
    TTTTCTGAGA ACCTCCAACC AGTTAATAAT ACCATTCCAC ATCTGTCCAC TGAGACCCTT
    GAGCTGCAAG GCATGGTTGT CACAGAACAT AGCCACGCAG ACTATAATAA GAACTTCAGC
    CGTCTAGGAA ACCTCACAGC TAATGTGCTG ATTGATAACA TTGAGACTCT GCCTCCAAAC
    TCAACCATTG TCAGTGTGGC CTACTCAGCA CTTGGTCATA TTTTGCCTGT GAAGGAGAAC
    ATGTCTGTGA ACAGTTTAGT GATATCAACA GCTCTGAGCT GCGAGAGAAA CCAGAACTTC
    CATATTAATA TGACCTTTCA GAAAGAAAAC ATATCTCTGA AGATGCCCCT GTGTGTCTTT
    TGGGACTTCA AACTCAACGA AAGTAGGGGA GGCTGGAATG ATCGTGGTTG CACGCAAACA
    GAGGAAGGCA GCAATGTCAT TTGTTCCTGC AATCACTTGA CACCATTCTC CATTCTGATG
    TCACCGGATG AACCTGCTCA CAATTCTGTT GAAGATTACA TCACCCACAT TGGCCTGGCC
    ATTTCAATCT TGAGTTTAGT GGTCTGCATT GTAATTGAAT CCTTAGTTTG GAAAACTGTG
    ACAAACAACA CAACCTCCTA CATGCGTCAC GTCTGTATTC TCAACACAGC GACATCTCTC
    CTAATTGCTG ACATTTGGTT CATTGTTACT GCCTCCATCA ATGTGCAGAA AAGAAATATC
    TGTATTGCAG CCACATTCTT CATCCATTTA TTCTACCTAT GTGGCTTTTT CTGGATGCTC
    AGCCTCGGCC TCATCCTTTT TTACAGACTA GTCTTCATCT TACATAACAC AAGCAAGACA
    TCTCAGAAAG CAGTGGTATT CTGCCTGGGT TATGGGTGCC CCTTTGTGTT TGCAGTCACC
    ACCATTGCTG TCACATTGCC GTGGAACACT TACACCAGAA AGGATGCCTG CTGGCTCAAC
    TGGAAGGACA GCAAAGCTGT CTTGGCCTTT GTCATACCTG CCCTGGCCAT CGTGGCCATG
    AATTTGTTCA TCACTGCAGT TGTGATAATA AAAATACTAA GGCCGAACAT TGGGGACAGG
    ACAAACAAAC AAGAGAGGAA GACCTTCTTT CAAATTTGCA AAAGTCTTGT CATCTTGACA
    CCACTGTTAG GCCTCACGTG GGGATTTGGT CTTGCCACCA TCATCAGAAA TAGCCACCAG
    ACTTTCCACA TCCTCTTTGC TCTGCTCAAT GCCTTGCAGG GACTGTTCAT TTTGGTATTT
    GGGACTCTAT GGGACAACAA GATAAAAGAA GCCCTGCTGA AGAGGAATTC ACTCTCCAGA
    TGGAGTTCGC AACAAACAAA GTCAACATCC CTGATCTTGG TGACACCAAT GTTTTCCATG
    AGCTATCCAT TTTCAAGAAC ATTCAACAAC TTATGTGGCA AAACAGGAAA ATACACAGTA
    TCTTCTTCAG AGCCATCCAG TGCTTCCACA GAGAACATCT CCAAGTCCGA TTCTTTGCTC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005052.1
    CDS301..4026
    Translation

    Target ORF information:

    RefSeq Version XM_025149284.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149284.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACAGTTT CAAGTATCAA CATCACAACA GTCTGTGTGC CCAGTGAGAA CAAGAGCTGC 
    TGTTCTTGTG AAAGCGGATA TGCCTGGCCA AGCACGGGGT GCAGTGACCT GATCAGCTGC
    CCTGCTCTCA GCCGAGCTCC CAACCAGCCA TGCGGCTACA CCAGGGAAAG GCCTTTCAAT
    GGGCCCTACT GTGAACCTCA GAATGGAGAC TCATGTGGTA TGGGAGAGCC CACTGTAATG
    AATATGTCAG TAAGACTTGA CACAGCCTTC CACGATGATC TCAGGAATCA TTCTTCCCAA
    CTATACAGAA AATACAAAGC TGACCTCGAT AAAGCGTTTA AAGCTAGCTA CGGGTGCTTA
    CCGGGCTTTG TGTCAGCAAC AACAACTGGT TTCAGGCCTG GGAGTGTTGA GGTGAACTAC
    AGAGTGGAGG CAGGATCAGC GAGCTTCCAA CAGCTGGAGC ATTCCAGCAG ATTTGTTACA
    CAGTACCTGG ATGAACCATA CAGCATAGTG CCTACCTCCT TTACAGCAGA AATAACGAAT
    CGCACAAACT TCACAGTGAG CCCTGCCCAC ATTTTTGAAG GGGATACCAT CCAGATGGTG
    TGTGAGATAA ACACCACGTC TGAGAACATG ACCTGGTATC GTGACAGTCA GATCATCCTC
    AACAGCTCAC GGCACTCCGT GAGGACAGAC AACAACACTG CGATGTCACA GGCATTTCTT
    ACAATTACCA ACGTTACGCA GGAGGATTCT GGTACCTACA AATGCACTTT TGCAAACAGA
    TACCGGTCTT TAAATCTGAT ATACGAAGGC ACCAAAGAAA TACAAGTCTC TCCACTACGT
    ATCATCTCAT ATTCAAACGA CTATAAAGTT CACTGCAACA GCCCTGAGAT ACAGGCAAAC
    AGCCCTGTGC TGTTCTGTTG CATTGATGGA CAATTACCAC TGCTTACTGG TGACTGGAAG
    GTGAATGGAG CGATCAGTAT TACAGGAGTC CCCAGTTTCA GCAGCAACTG CACTGAATAC
    AAGCTCAACA TGACGGACTC GCTGTGCTTG GCCGAGCATT CAGGTACGGT GACGACATAC
    ACGTGTGAGC TGCAGACAGG GCACGGCGCC AGGAGCAGCC GGGACATCCG AGTGATGTAC
    TTCCGAGCAG CCCAGGTAAG AATATCTTCC AGCGTAAACA CAACCGTTTC CGTGGGATAT
    GGCTTCAGTC TAAAGTGTGA AAGCGATACG AGCAATTACG ACAGCATCAG CTGGGAAATC
    CATTCTGGAG ATAGAATACA AGCAGTAGAA TGCGCTGCAT GCATTAGAAC CAACAAATCC
    ATGGCCACAT CCATGCTCGT GGTGAACACT GCCACTCAAG ACTGGAATGG CACCTACATC
    TGCACATTGT CCCAGGACTC CCTGAACAGT TCTGCCAGCG TGACAATCAA TGTCATTCCG
    TTGCCCCTGA AGCAGCACAT CCTGATAGAC CCCGCTGGAG CGCCTATCAT GTGCGGAACG
    CCCCAAGCCC TCACCTGCTG CATCAGTGCT AACAGCATGG GAGATTATGA TGTTAAATTT
    GTGGTACAAG AAAAAGAATT TCAAGCTGAG AAAGAAGAGC AAGGAAATTT CTTTTGCTAC
    TCGTACAATT ACACAGAAAC AGAATGCAGG GAAAAGAAGC TGGAGGCGTA TTGCAAGTTT
    GTGAACAGCA ATGAGCAGGA GGTCAGCAGC AACAGTATAA ATCTGAGCCT GATACCTGAT
    AAGGACCTCA TTTCCTGCTC AGAAAACAAT ACCTTTGGAA AAGAAGGAGA CATATTGAAA
    AAGTCGTGCT CTGTTTTAAA TACTACTTTT ATCCGAGGCA GCAACATTTA CAAGTGCTTC
    AACAGCTCAT GGAAGATGGA AAAGAATGAC TGCGTATCCG AACAGATAAA CAACCTGCTG
    CTCATGGCAG AGTCCTTGGT CAACAGTCCC AGTGCAAAAG TTGGTCTACC TAACTTCCTT
    CAGAACCTGC GGAATCAGAC AGAACTGCTG CAAAATGACA ATTCTTCTGC AAATCTTGCT
    GCAATTGTTA CCATCCTCTA CAACATCTCT TCTATTCCAG TAGAAGCAAA GCAGTCCACT
    ATCTCTGATT ACTTCACTAC AGTGGACATT ATCGTTACTG ATTTCAAAAA GGAACTTTGG
    GCTGACCTGA ATGAAGGGCA GGTACCCATA AGTTCTTTGT TACTGAATGC GGTGGAAAGA
    TTTTCTGAGA ACCTCCAACC AGTTAATAAT ACCATTCCAC ATCTGTCCAC TGAGACCCTT
    GAGCTGCAAG GCATGGTTGT CACAGAACAT AGCCACGCAG ACTATAATAA GAACTTCAGC
    CGTCTAGGAA ACCTCACAGC TAATGTGCTG ATTGATAACA TTGAGACTCT GCCTCCAAAC
    TCAACCATTG TCAGTGTGGC CTACTCAGCA CTTGGTCATA TTTTGCCTGT GAAGGAGAAC
    ATGTCTGTGA ACAGTTTAGT GATATCAACA GCTCTGAGCT GCGAGAGAAA CCAGAACTTC
    CATATTAATA TGACCTTTCA GAAAGAAAAC ATATCTCTGA AGATGCCCCT GTGTGTCTTT
    TGGGACTTCA AACTCAACGA AAGTAGGGGA GGCTGGAATG ATCGTGGTTG CACGCAAACA
    GAGGAAGGCA GCAATGTCAT TTGTTCCTGC AATCACTTGA CACCATTCTC CATTCTGATG
    TCACCGGATG AACCTGCTCA CAATTCTGTT GAAGATTACA TCACCCACAT TGGCCTGGCC
    ATTTCAATCT TGAGTTTAGT GGTCTGCATT GTAATTGAAT CCTTAGTTTG GAAAACTGTG
    ACAAACAACA CAACCTCCTA CATGCGTCAC GTCTGTATTC TCAACACAGC GACATCTCTC
    CTAATTGCTG ACATTTGGTT CATTGTTACT GCCTCCATCA ATGTGCAGAA AAGAAATATC
    TGTATTGCAG CCACATTCTT CATCCATTTA TTCTACCTAT GTGGCTTTTT CTGGATGCTC
    AGCCTCGGCC TCATCCTTTT TTACAGACTA GTCTTCATCT TACATAACAC AAGCAAGACA
    TCTCAGAAAG CAGTGGTATT CTGCCTGGGT TATGGGTGCC CCTTTGTGTT TGCAGTCACC
    ACCATTGCTG TCACATTGCC GTGGAACACT TACACCAGAA AGGATGCCTG CTGGCTCAAC
    TGGAAGGACA GCAAAGCTGT CTTGGCCTTT GTCATACCTG CCCTGGCCAT CGTGGCCATG
    AATTTGTTCA TCACTGCAGT TGTGATAATA AAAATACTAA GGCCGAACAT TGGGGACAGG
    ACAAACAAAC AAGAGAGGAA GACCTTCTTT CAAATTTGCA AAAGTCTTGT CATCTTGACA
    CCACTGTTAG GCCTCACGTG GGGATTTGGT CTTGCCACCA TCATCAGAAA TAGCCACCAG
    ACTTTCCACA TCCTCTTTGC TCTGCTCAAT GCCTTGCAGG GACTGTTCAT TTTGGTATTT
    GGGACTCTAT GGGACAACAA GATAAAAGAA GCCCTGCTGA AGAGGAATTC ACTCTCCAGA
    TGGAGTTCGC AACAAACAAA GTCAACATCC CTGATCTTGG TGACACCAAT GTTTTCCATG
    AGCTATCCAT TTTCAAGAAC ATTCAACAAC TTATGTGGCA AAACAGGAAA ATACACAGTA
    TCTTCTTCAG AGCCATCCAG TGCTTCCACA GAGAACATCT CCAAGTCCGA TTCTTTGCTC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34448
    Clone ID Related Accession (Same CDS sequence) XM_015285168.1
    Accession Version XM_015285168.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product adhesion G protein-coupled receptor F5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCCATCCC CAATTACTGC AGCTCTCCAC CTCCTGTCTC TACTGGCCGT ATGCTTCCAG 
    ACGTCTCCAG CCTCGGGCTT TGACTCATTC ACCCAATATG CCGTTGGCAG GGAGGTAGGA
    GAGGAGAGCT TCCACCCAGA GCTGCAGAGG CAGAAGCGAC ATGTGATCCT TTTTGACCTT
    CCACCCCTGG AGTATGCCAT TGACATTGAA GTAAGCCTCA CGAATTCATC CTTCCTGGCA
    CCAATCAAGG AGTACTTCAG AAACCTCCAT CTTCCAGTTT CAATCAATAT TTCAGATGTA
    GAAATGACAG TTTCAAGTAT CAACATCACA ACAGTCTGTG TGCCCAGTGA GAACAAGAGC
    TGCTGTTCTT GTGAAAGCGG ATATGCCTGG CCAAGCACGG GGTGCAGTGA CCTGATCAGC
    TGCCCTGCTC TCAGCCGAGC TCCCAACCAG CCATGCGGCT ACACCAGGGA AAGGCCTTTC
    AATGGGCCCT ACTGTGAACC TCAGAATGGA GACTCATGTG GTATGGGAGA GCCCACTGTA
    ATGAATATGT CAGTAAGACT TGACACAGCC TTCCACGATG ATCTCAGGAA TCATTCTTCC
    CAACTATACA GAAAATACAA AGCTGACCTC GATAAAGCGT TTAAAGCTAG CTACGGGTGC
    TTACCGGGCT TTGTGTCAGC AACAACAACT GGTTTCAGGC CTGGGAGTGT TGAGGTGAAC
    TACAGAGTGG AGGCAGGATC AGCGAGCTTC CAACAGCTGG AGCATTCCAG CAGATTTGTT
    ACACAGTACC TGGATGAACC ATACAGCATA GTGCCTACCT CCTTTACAGC AGAAATAACG
    AATCGCACAA ACTTCACAGT GAGCCCTGCC CACATTTTTG AAGGGGATAC CATCCAGATG
    GTGTGTGAGA TAAACACCAC GTCTGAGAAC ATGACCTGGT ATCGTGACAG TCAGATCATC
    CTCAACAGCT CACGGCACTC CGTGAGGACA GACAACAACA CTGCGATGTC ACAGGCATTT
    CTTACAATTA CCAACGTTAC GCAGGAGGAT TCTGGTACCT ACAAATGCAC TTTTGCAAAC
    AGATACCGGT CTTTAAATCT GATATACGAA GGCACCAAAG AAATACAAGT CTCTCCACTA
    CGTATCATCT CATATTCAAA CGACTATAAA GTTCACTGCA ACAGCCCTGA GATACAGGCA
    AACAGCCCTG TGCTGTTCTG TTGCATTGAT GGACAATTAC CACTGCTTAC TGGTGACTGG
    AAGGTGAATG GAGCGATCAG TATTACAGGA GTCCCCAGTT TCAGCAGCAA CTGCACTGAA
    TACAAGCTCA ACATGACGGA CTCGCTGTGC TTGGCCGAGC ATTCAGGTAC GGTGACGACA
    TACACGTGTG AGCTGCAGAC AGGGCACGGC GCCAGGAGCA GCCGGGACAT CCGAGTGATG
    TACTTCCGAG CAGCCCAGGT AAGAATATCT TCCAGCGTAA ACACAACCGT TTCCGTGGGA
    TATGGCTTCA GTCTAAAGTG TGAAAGCGAT ACGAGCAATT ACGACAGCAT CAGCTGGGAA
    ATCCATTCTG GAGATAGAAT ACAAGCAGTA GAATGCGCTG CATGCATTAG AACCAACAAA
    TCCATGGCCA CATCCATGCT CGTGGTGAAC ACTGCCACTC AAGACTGGAA TGGCACCTAC
    ATCTGCACAT TGTCCCAGGA CTCCCTGAAC AGTTCTGCCA GCGTGACAAT CAATGTCATT
    CCGTTGCCCC TGAAGCAGCA CATCCTGATA GACCCCGCTG GAGCGCCTAT CATGTGCGGA
    ACGCCCCAAG CCCTCACCTG CTGCATCAGT GCTAACAGCA TGGGAGATTA TGATGTTAAA
    TTTGTGGTAC AAGAAAAAGA ATTTCAAGCT GAGAAAGAAG AGCAAGGAAA TTTCTTTTGC
    TACTCGTACA ATTACACAGA AACAGAATGC AGGGAAAAGA AGCTGGAGGC GTATTGCAAG
    TTTGTGAACA GCAATGAGCA GGAGGTCAGC AGCAACAGTA TAAATCTGAG CCTGATACCT
    GATAAGGACC TCATTTCCTG CTCAGAAAAC AATACCTTTG GAAAAGAAGG AGACATATTG
    AAAAAGTCGT GCTCTGTTTT AAATACTACT TTTATCCGAG GCAGCAACAT TTACAAGTGC
    TTCAACAGCT CATGGAAGAT GGAAAAGAAT GACTGCGTAT CCGAACAGAT AAACAACCTG
    CTGCTCATGG CAGAGTCCTT GGTCAACAGT CCCAGTGCAA AAGTTGGTCT ACCTAACTTC
    CTTCAGAACC TGCGGAATCA GACAGAACTG CTGCAAAATG ACAATTCTTC TGCAAATCTT
    GCTGCAATTG TTACCATCCT CTACAACATC TCTTCTATTC CAGTAGAAGC AAAGCAGTCC
    ACTATCTCTG ATTACTTCAC TACAGTGGAC ATTATCGTTA CTGATTTCAA AAAGGAACTT
    TGGGCTGACC TGAATGAAGG GCAGGTACCC ATAAGTTCTT TGTTACTGAA TGCGGTGGAA
    AGATTTTCTG AGAACCTCCA ACCAGTTAAT AATACCATTC CACATCTGTC CACTGAGACC
    CTTGAGCTGC AAGGCATGGT TGTCACAGAA CATAGCCACG CAGACTATAA TAAGAACTTC
    AGCCGTCTAG GAAACCTCAC AGCTAATGTG CTGATTGATA ACATTGAGAC TCTGCCTCCA
    AACTCAACCA TTGTCAGTGT GGCCTACTCA GCACTTGGTC ATATTTTGCC TGTGAAGGAG
    AACATGTCTG TGAACAGTTT AGTGATATCA ACAGCTCTGA GCTGCGAGAG AAACCAGAAC
    TTCCATATTA ATATGACCTT TCAGAAAGAA AACATATCTC TGAAGATGCC CCTGTGTGTC
    TTTTGGGACT TCAAACTCAA CGAAAGTAGG GGAGGCTGGA ATGATCGTGG TTGCACGCAA
    ACAGAGGAAG GCAGCAATGT CATTTGTTCC TGCAATCACT TGACACCATT CTCCATTCTG
    ATGTCACCGG ATGAACCTGC TCACAATTCT GTTGAAGATT ACATCACCCA CATTGGCCTG
    GCCATTTCAA TCTTGAGTTT AGTGGTCTGC ATTGTAATTG AATCCTTAGT TTGGAAAACT
    GTGACAAACA ACACAACCTC CTACATGCGT CACGTCTGTA TTCTCAACAC AGCGACATCT
    CTCCTAATTG CTGACATTTG GTTCATTGTT ACTGCCTCCA TCAATGTGCA GAAAAGAAAT
    ATCTGTATTG CAGCCACATT CTTCATCCAT TTATTCTACC TATGTGGCTT TTTCTGGATG
    CTCAGCCTCG GCCTCATCCT TTTTTACAGA CTAGTCTTCA TCTTACATAA CACAAGCAAG
    ACATCTCAGA AAGCAGTGGT ATTCTGCCTG GGTTATGGGT GCCCCTTTGT GTTTGCAGTC
    ACCACCATTG CTGTCACATT GCCGTGGAAC ACTTACACCA GAAAGGATGC CTGCTGGCTC
    AACTGGAAGG ACAGCAAAGC TGTCTTGGCC TTTGTCATAC CTGCCCTGGC CATCGTGGCC
    ATGAATTTGT TCATCACTGC AGTTGTGATA ATAAAAATAC TAAGGCCGAA CATTGGGGAC
    AGGACAAACA AACAAGAGAG GAAGACCTTC TTTCAAATTT GCAAAAGTCT TGTCATCTTG
    ACACCACTGT TAGGCCTCAC GTGGGGATTT GGTCTTGCCA CCATCATCAG AAATAGCCAC
    CAGACTTTCC ACATCCTCTT TGCTCTGCTC AATGCCTTGC AGGTATTCAG TCTGTCCTGG
    AGGACTCCAA CCACATCAAC AGTCCATCAT TATGTGAAAT GCACTCTGAA AACGCCTTCT
    ATCCAAGAGT TTGGAGTTTT AATGGTCAGC ATGGACAATG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140654.1
    CDS446..4330
    Translation

    Target ORF information:

    RefSeq Version XM_015285168.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285168.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCCATCCC CAATTACTGC AGCTCTCCAC CTCCTGTCTC TACTGGCCGT ATGCTTCCAG 
    ACGTCTCCAG CCTCGGGCTT TGACTCATTC ACCCAATATG CCGTTGGCAG GGAGGTAGGA
    GAGGAGAGCT TCCACCCAGA GCTGCAGAGG CAGAAGCGAC ATGTGATCCT TTTTGACCTT
    CCACCCCTGG AGTATGCCAT TGACATTGAA GTAAGCCTCA CGAATTCATC CTTCCTGGCA
    CCAATCAAGG AGTACTTCAG AAACCTCCAT CTTCCAGTTT CAATCAATAT TTCAGATGTA
    GAAATGACAG TTTCAAGTAT CAACATCACA ACAGTCTGTG TGCCCAGTGA GAACAAGAGC
    TGCTGTTCTT GTGAAAGCGG ATATGCCTGG CCAAGCACGG GGTGCAGTGA CCTGATCAGC
    TGCCCTGCTC TCAGCCGAGC TCCCAACCAG CCATGCGGCT ACACCAGGGA AAGGCCTTTC
    AATGGGCCCT ACTGTGAACC TCAGAATGGA GACTCATGTG GTATGGGAGA GCCCACTGTA
    ATGAATATGT CAGTAAGACT TGACACAGCC TTCCACGATG ATCTCAGGAA TCATTCTTCC
    CAACTATACA GAAAATACAA AGCTGACCTC GATAAAGCGT TTAAAGCTAG CTACGGGTGC
    TTACCGGGCT TTGTGTCAGC AACAACAACT GGTTTCAGGC CTGGGAGTGT TGAGGTGAAC
    TACAGAGTGG AGGCAGGATC AGCGAGCTTC CAACAGCTGG AGCATTCCAG CAGATTTGTT
    ACACAGTACC TGGATGAACC ATACAGCATA GTGCCTACCT CCTTTACAGC AGAAATAACG
    AATCGCACAA ACTTCACAGT GAGCCCTGCC CACATTTTTG AAGGGGATAC CATCCAGATG
    GTGTGTGAGA TAAACACCAC GTCTGAGAAC ATGACCTGGT ATCGTGACAG TCAGATCATC
    CTCAACAGCT CACGGCACTC CGTGAGGACA GACAACAACA CTGCGATGTC ACAGGCATTT
    CTTACAATTA CCAACGTTAC GCAGGAGGAT TCTGGTACCT ACAAATGCAC TTTTGCAAAC
    AGATACCGGT CTTTAAATCT GATATACGAA GGCACCAAAG AAATACAAGT CTCTCCACTA
    CGTATCATCT CATATTCAAA CGACTATAAA GTTCACTGCA ACAGCCCTGA GATACAGGCA
    AACAGCCCTG TGCTGTTCTG TTGCATTGAT GGACAATTAC CACTGCTTAC TGGTGACTGG
    AAGGTGAATG GAGCGATCAG TATTACAGGA GTCCCCAGTT TCAGCAGCAA CTGCACTGAA
    TACAAGCTCA ACATGACGGA CTCGCTGTGC TTGGCCGAGC ATTCAGGTAC GGTGACGACA
    TACACGTGTG AGCTGCAGAC AGGGCACGGC GCCAGGAGCA GCCGGGACAT CCGAGTGATG
    TACTTCCGAG CAGCCCAGGT AAGAATATCT TCCAGCGTAA ACACAACCGT TTCCGTGGGA
    TATGGCTTCA GTCTAAAGTG TGAAAGCGAT ACGAGCAATT ACGACAGCAT CAGCTGGGAA
    ATCCATTCTG GAGATAGAAT ACAAGCAGTA GAATGCGCTG CATGCATTAG AACCAACAAA
    TCCATGGCCA CATCCATGCT CGTGGTGAAC ACTGCCACTC AAGACTGGAA TGGCACCTAC
    ATCTGCACAT TGTCCCAGGA CTCCCTGAAC AGTTCTGCCA GCGTGACAAT CAATGTCATT
    CCGTTGCCCC TGAAGCAGCA CATCCTGATA GACCCCGCTG GAGCGCCTAT CATGTGCGGA
    ACGCCCCAAG CCCTCACCTG CTGCATCAGT GCTAACAGCA TGGGAGATTA TGATGTTAAA
    TTTGTGGTAC AAGAAAAAGA ATTTCAAGCT GAGAAAGAAG AGCAAGGAAA TTTCTTTTGC
    TACTCGTACA ATTACACAGA AACAGAATGC AGGGAAAAGA AGCTGGAGGC GTATTGCAAG
    TTTGTGAACA GCAATGAGCA GGAGGTCAGC AGCAACAGTA TAAATCTGAG CCTGATACCT
    GATAAGGACC TCATTTCCTG CTCAGAAAAC AATACCTTTG GAAAAGAAGG AGACATATTG
    AAAAAGTCGT GCTCTGTTTT AAATACTACT TTTATCCGAG GCAGCAACAT TTACAAGTGC
    TTCAACAGCT CATGGAAGAT GGAAAAGAAT GACTGCGTAT CCGAACAGAT AAACAACCTG
    CTGCTCATGG CAGAGTCCTT GGTCAACAGT CCCAGTGCAA AAGTTGGTCT ACCTAACTTC
    CTTCAGAACC TGCGGAATCA GACAGAACTG CTGCAAAATG ACAATTCTTC TGCAAATCTT
    GCTGCAATTG TTACCATCCT CTACAACATC TCTTCTATTC CAGTAGAAGC AAAGCAGTCC
    ACTATCTCTG ATTACTTCAC TACAGTGGAC ATTATCGTTA CTGATTTCAA AAAGGAACTT
    TGGGCTGACC TGAATGAAGG GCAGGTACCC ATAAGTTCTT TGTTACTGAA TGCGGTGGAA
    AGATTTTCTG AGAACCTCCA ACCAGTTAAT AATACCATTC CACATCTGTC CACTGAGACC
    CTTGAGCTGC AAGGCATGGT TGTCACAGAA CATAGCCACG CAGACTATAA TAAGAACTTC
    AGCCGTCTAG GAAACCTCAC AGCTAATGTG CTGATTGATA ACATTGAGAC TCTGCCTCCA
    AACTCAACCA TTGTCAGTGT GGCCTACTCA GCACTTGGTC ATATTTTGCC TGTGAAGGAG
    AACATGTCTG TGAACAGTTT AGTGATATCA ACAGCTCTGA GCTGCGAGAG AAACCAGAAC
    TTCCATATTA ATATGACCTT TCAGAAAGAA AACATATCTC TGAAGATGCC CCTGTGTGTC
    TTTTGGGACT TCAAACTCAA CGAAAGTAGG GGAGGCTGGA ATGATCGTGG TTGCACGCAA
    ACAGAGGAAG GCAGCAATGT CATTTGTTCC TGCAATCACT TGACACCATT CTCCATTCTG
    ATGTCACCGG ATGAACCTGC TCACAATTCT GTTGAAGATT ACATCACCCA CATTGGCCTG
    GCCATTTCAA TCTTGAGTTT AGTGGTCTGC ATTGTAATTG AATCCTTAGT TTGGAAAACT
    GTGACAAACA ACACAACCTC CTACATGCGT CACGTCTGTA TTCTCAACAC AGCGACATCT
    CTCCTAATTG CTGACATTTG GTTCATTGTT ACTGCCTCCA TCAATGTGCA GAAAAGAAAT
    ATCTGTATTG CAGCCACATT CTTCATCCAT TTATTCTACC TATGTGGCTT TTTCTGGATG
    CTCAGCCTCG GCCTCATCCT TTTTTACAGA CTAGTCTTCA TCTTACATAA CACAAGCAAG
    ACATCTCAGA AAGCAGTGGT ATTCTGCCTG GGTTATGGGT GCCCCTTTGT GTTTGCAGTC
    ACCACCATTG CTGTCACATT GCCGTGGAAC ACTTACACCA GAAAGGATGC CTGCTGGCTC
    AACTGGAAGG ACAGCAAAGC TGTCTTGGCC TTTGTCATAC CTGCCCTGGC CATCGTGGCC
    ATGAATTTGT TCATCACTGC AGTTGTGATA ATAAAAATAC TAAGGCCGAA CATTGGGGAC
    AGGACAAACA AACAAGAGAG GAAGACCTTC TTTCAAATTT GCAAAAGTCT TGTCATCTTG
    ACACCACTGT TAGGCCTCAC GTGGGGATTT GGTCTTGCCA CCATCATCAG AAATAGCCAC
    CAGACTTTCC ACATCCTCTT TGCTCTGCTC AATGCCTTGC AGGTATTCAG TCTGTCCTGG
    AGGACTCCAA CCACATCAAC AGTCCATCAT TATGTGAAAT GCACTCTGAA AACGCCTTCT
    ATCCAAGAGT TTGGAGTTTT AATGGTCAGC ATGGACAATG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07171
    Clone ID Related Accession (Same CDS sequence) XM_004935931.2 , XM_015285166.1 , XM_004935931.3 , XM_015285167.1 , XM_004935933.2 , XM_004935933.3 , XM_015285166.2 , XM_025149283.1 , XM_015285167.2
    Accession Version XM_004935931.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product adhesion G protein-coupled receptor F5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004935931.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCCATCCC CAATTACTGC AGCTCTCCAC CTCCTGTCTC TACTGGCCGT ATGCTTCCAG 
    ACGTCTCCAG CCTCGGGCTT TGACTCATTC ACCCAATATG CCGTTGGCAG GGAGGTAGGA
    GAGGAGAGCT TCCACCCAGA GCTGCAGAGG CAGAAGCGAC ATGTGATCCT TTTTGACCTT
    CCACCCCTGG AGTATGCCAT TGACATTGAA GTAAGCCTCA CGAATTCATC CTTCCTGGCA
    CCAATCAAGG AGTACTTCAG AAACCTCCAT CTTCCAGTTT CAATCAATAT TTCAGATGTA
    GAAATGACAG TTTCAAGTAT CAACATCACA ACAGTCTGTG TGCCCAGTGA GAACAAGAGC
    TGCTGTTCTT GTGAAAGCGG ATATGCCTGG CCAAGCACGG GGTGCAGTGA CCTGATCAGC
    TGCCCTGCTC TCAGCCGAGC TCCCAACCAG CCATGCGGCT ACACCAGGGA AAGGCCTTTC
    AATGGGCCCT ACTGTGAACC TCAGAATGGA GACTCATGTG GTATGGGAGA GCCCACTGTA
    ATGAATATGT CAGTAAGACT TGACACAGCC TTCCACGATG ATCTCAGGAA TCATTCTTCC
    CAACTATACA GAAAATACAA AGCTGACCTC GATAAAGCGT TTAAAGCTAG CTACGGGTGC
    TTACCGGGCT TTGTGTCAGC AACAACAACT GGTTTCAGGC CTGGGAGTGT TGAGGTGAAC
    TACAGAGTGG AGGCAGGATC AGCGAGCTTC CAACAGCTGG AGCATTCCAG CAGATTTGTT
    ACACAGTACC TGGATGAACC ATACAGCATA GTGCCTACCT CCTTTACAGC AGAAATAACG
    AATCGCACAA ACTTCACAGT GAGCCCTGCC CACATTTTTG AAGGGGATAC CATCCAGATG
    GTGTGTGAGA TAAACACCAC GTCTGAGAAC ATGACCTGGT ATCGTGACAG TCAGATCATC
    CTCAACAGCT CACGGCACTC CGTGAGGACA GACAACAACA CTGCGATGTC ACAGGCATTT
    CTTACAATTA CCAACGTTAC GCAGGAGGAT TCTGGTACCT ACAAATGCAC TTTTGCAAAC
    AGATACCGGT CTTTAAATCT GATATACGAA GGCACCAAAG AAATACAAGT CTCTCCACTA
    CGTATCATCT CATATTCAAA CGACTATAAA GTTCACTGCA ACAGCCCTGA GATACAGGCA
    AACAGCCCTG TGCTGTTCTG TTGCATTGAT GGACAATTAC CACTGCTTAC TGGTGACTGG
    AAGGTGAATG GAGCGATCAG TATTACAGGA GTCCCCAGTT TCAGCAGCAA CTGCACTGAA
    TACAAGCTCA ACATGACGGA CTCGCTGTGC TTGGCCGAGC ATTCAGGTAC GGTGACGACA
    TACACGTGTG AGCTGCAGAC AGGGCACGGC GCCAGGAGCA GCCGGGACAT CCGAGTGATG
    TACTTCCGAG CAGCCCAGGT AAGAATATCT TCCAGCGTAA ACACAACCGT TTCCGTGGGA
    TATGGCTTCA GTCTAAAGTG TGAAAGCGAT ACGAGCAATT ACGACAGCAT CAGCTGGGAA
    ATCCATTCTG GAGATAGAAT ACAAGCAGTA GAATGCGCTG CATGCATTAG AACCAACAAA
    TCCATGGCCA CATCCATGCT CGTGGTGAAC ACTGCCACTC AAGACTGGAA TGGCACCTAC
    ATCTGCACAT TGTCCCAGGA CTCCCTGAAC AGTTCTGCCA GCGTGACAAT CAATGTCATT
    CCGTTGCCCC TGAAGCAGCA CATCCTGATA GACCCCGCTG GAGCGCCTAT CATGTGCGGA
    ACGCCCCAAG CCCTCACCTG CTGCATCAGT GCTAACAGCA TGGGAGATTA TGATGTTAAA
    TTTGTGGTAC AAGAAAAAGA ATTTCAAGCT GAGAAAGAAG AGCAAGGAAA TTTCTTTTGC
    TACTCGTACA ATTACACAGA AACAGAATGC AGGGAAAAGA AGCTGGAGGC GTATTGCAAG
    TTTGTGAACA GCAATGAGCA GGAGGTCAGC AGCAACAGTA TAAATCTGAG CCTGATACCT
    GATAAGGACC TCATTTCCTG CTCAGAAAAC AATACCTTTG GAAAAGAAGG AGACATATTG
    AAAAAGTCGT GCTCTGTTTT AAATACTACT TTTATCCGAG GCAGCAACAT TTACAAGTGC
    TTCAACAGCT CATGGAAGAT GGAAAAGAAT GACTGCGTAT CCGAACAGAT AAACAACCTG
    CTGCTCATGG CAGAGTCCTT GGTCAACAGT CCCAGTGCAA AAGTTGGTCT ACCTAACTTC
    CTTCAGAACC TGCGGAATCA GACAGAACTG CTGCAAAATG ACAATTCTTC TGCAAATCTT
    GCTGCAATTG TTACCATCCT CTACAACATC TCTTCTATTC CAGTAGAAGC AAAGCAGTCC
    ACTATCTCTG ATTACTTCAC TACAGTGGAC ATTATCGTTA CTGATTTCAA AAAGGAACTT
    TGGGCTGACC TGAATGAAGG GCAGGTACCC ATAAGTTCTT TGTTACTGAA TGCGGTGGAA
    AGATTTTCTG AGAACCTCCA ACCAGTTAAT AATACCATTC CACATCTGTC CACTGAGACC
    CTTGAGCTGC AAGGCATGGT TGTCACAGAA CATAGCCACG CAGACTATAA TAAGAACTTC
    AGCCGTCTAG GAAACCTCAC AGCTAATGTG CTGATTGATA ACATTGAGAC TCTGCCTCCA
    AACTCAACCA TTGTCAGTGT GGCCTACTCA GCACTTGGTC ATATTTTGCC TGTGAAGGAG
    AACATGTCTG TGAACAGTTT AGTGATATCA ACAGCTCTGA GCTGCGAGAG AAACCAGAAC
    TTCCATATTA ATATGACCTT TCAGAAAGAA AACATATCTC TGAAGATGCC CCTGTGTGTC
    TTTTGGGACT TCAAACTCAA CGAAAGTAGG GGAGGCTGGA ATGATCGTGG TTGCACGCAA
    ACAGAGGAAG GCAGCAATGT CATTTGTTCC TGCAATCACT TGACACCATT CTCCATTCTG
    ATGTCACCGG ATGAACCTGC TCACAATTCT GTTGAAGATT ACATCACCCA CATTGGCCTG
    GCCATTTCAA TCTTGAGTTT AGTGGTCTGC ATTGTAATTG AATCCTTAGT TTGGAAAACT
    GTGACAAACA ACACAACCTC CTACATGCGT CACGTCTGTA TTCTCAACAC AGCGACATCT
    CTCCTAATTG CTGACATTTG GTTCATTGTT ACTGCCTCCA TCAATGTGCA GAAAAGAAAT
    ATCTGTATTG CAGCCACATT CTTCATCCAT TTATTCTACC TATGTGGCTT TTTCTGGATG
    CTCAGCCTCG GCCTCATCCT TTTTTACAGA CTAGTCTTCA TCTTACATAA CACAAGCAAG
    ACATCTCAGA AAGCAGTGGT ATTCTGCCTG GGTTATGGGT GCCCCTTTGT GTTTGCAGTC
    ACCACCATTG CTGTCACATT GCCGTGGAAC ACTTACACCA GAAAGGATGC CTGCTGGCTC
    AACTGGAAGG ACAGCAAAGC TGTCTTGGCC TTTGTCATAC CTGCCCTGGC CATCGTGGCC
    ATGAATTTGT TCATCACTGC AGTTGTGATA ATAAAAATAC TAAGGCCGAA CATTGGGGAC
    AGGACAAACA AACAAGAGAG GAAGACCTTC TTTCAAATTT GCAAAAGTCT TGTCATCTTG
    ACACCACTGT TAGGCCTCAC GTGGGGATTT GGTCTTGCCA CCATCATCAG AAATAGCCAC
    CAGACTTTCC ACATCCTCTT TGCTCTGCTC AATGCCTTGC AGGGACTGTT CATTTTGGTA
    TTTGGGACTC TATGGGACAA CAAGATAAAA GAAGCCCTGC TGAAGAGGAA TTCACTCTCC
    AGATGGAGTT CGCAACAAAC AAAGTCAACA TCCCTGATCT TGGTGACACC AATGTTTTCC
    ATGAGCTATC CATTTTCAAG AACATTCAAC AACTTATGTG GCAAAACAGG AAAATACACA
    GTATCTTCTT CAGAGCCATC CAGTGCTTCC ACAGAGAACA TCTCCAAGTC CGATTCTTTG
    CTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004935988.2
    CDS446..4474
    Translation

    Target ORF information:

    RefSeq Version XM_004935931.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004935931.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCCATCCC CAATTACTGC AGCTCTCCAC CTCCTGTCTC TACTGGCCGT ATGCTTCCAG 
    ACGTCTCCAG CCTCGGGCTT TGACTCATTC ACCCAATATG CCGTTGGCAG GGAGGTAGGA
    GAGGAGAGCT TCCACCCAGA GCTGCAGAGG CAGAAGCGAC ATGTGATCCT TTTTGACCTT
    CCACCCCTGG AGTATGCCAT TGACATTGAA GTAAGCCTCA CGAATTCATC CTTCCTGGCA
    CCAATCAAGG AGTACTTCAG AAACCTCCAT CTTCCAGTTT CAATCAATAT TTCAGATGTA
    GAAATGACAG TTTCAAGTAT CAACATCACA ACAGTCTGTG TGCCCAGTGA GAACAAGAGC
    TGCTGTTCTT GTGAAAGCGG ATATGCCTGG CCAAGCACGG GGTGCAGTGA CCTGATCAGC
    TGCCCTGCTC TCAGCCGAGC TCCCAACCAG CCATGCGGCT ACACCAGGGA AAGGCCTTTC
    AATGGGCCCT ACTGTGAACC TCAGAATGGA GACTCATGTG GTATGGGAGA GCCCACTGTA
    ATGAATATGT CAGTAAGACT TGACACAGCC TTCCACGATG ATCTCAGGAA TCATTCTTCC
    CAACTATACA GAAAATACAA AGCTGACCTC GATAAAGCGT TTAAAGCTAG CTACGGGTGC
    TTACCGGGCT TTGTGTCAGC AACAACAACT GGTTTCAGGC CTGGGAGTGT TGAGGTGAAC
    TACAGAGTGG AGGCAGGATC AGCGAGCTTC CAACAGCTGG AGCATTCCAG CAGATTTGTT
    ACACAGTACC TGGATGAACC ATACAGCATA GTGCCTACCT CCTTTACAGC AGAAATAACG
    AATCGCACAA ACTTCACAGT GAGCCCTGCC CACATTTTTG AAGGGGATAC CATCCAGATG
    GTGTGTGAGA TAAACACCAC GTCTGAGAAC ATGACCTGGT ATCGTGACAG TCAGATCATC
    CTCAACAGCT CACGGCACTC CGTGAGGACA GACAACAACA CTGCGATGTC ACAGGCATTT
    CTTACAATTA CCAACGTTAC GCAGGAGGAT TCTGGTACCT ACAAATGCAC TTTTGCAAAC
    AGATACCGGT CTTTAAATCT GATATACGAA GGCACCAAAG AAATACAAGT CTCTCCACTA
    CGTATCATCT CATATTCAAA CGACTATAAA GTTCACTGCA ACAGCCCTGA GATACAGGCA
    AACAGCCCTG TGCTGTTCTG TTGCATTGAT GGACAATTAC CACTGCTTAC TGGTGACTGG
    AAGGTGAATG GAGCGATCAG TATTACAGGA GTCCCCAGTT TCAGCAGCAA CTGCACTGAA
    TACAAGCTCA ACATGACGGA CTCGCTGTGC TTGGCCGAGC ATTCAGGTAC GGTGACGACA
    TACACGTGTG AGCTGCAGAC AGGGCACGGC GCCAGGAGCA GCCGGGACAT CCGAGTGATG
    TACTTCCGAG CAGCCCAGGT AAGAATATCT TCCAGCGTAA ACACAACCGT TTCCGTGGGA
    TATGGCTTCA GTCTAAAGTG TGAAAGCGAT ACGAGCAATT ACGACAGCAT CAGCTGGGAA
    ATCCATTCTG GAGATAGAAT ACAAGCAGTA GAATGCGCTG CATGCATTAG AACCAACAAA
    TCCATGGCCA CATCCATGCT CGTGGTGAAC ACTGCCACTC AAGACTGGAA TGGCACCTAC
    ATCTGCACAT TGTCCCAGGA CTCCCTGAAC AGTTCTGCCA GCGTGACAAT CAATGTCATT
    CCGTTGCCCC TGAAGCAGCA CATCCTGATA GACCCCGCTG GAGCGCCTAT CATGTGCGGA
    ACGCCCCAAG CCCTCACCTG CTGCATCAGT GCTAACAGCA TGGGAGATTA TGATGTTAAA
    TTTGTGGTAC AAGAAAAAGA ATTTCAAGCT GAGAAAGAAG AGCAAGGAAA TTTCTTTTGC
    TACTCGTACA ATTACACAGA AACAGAATGC AGGGAAAAGA AGCTGGAGGC GTATTGCAAG
    TTTGTGAACA GCAATGAGCA GGAGGTCAGC AGCAACAGTA TAAATCTGAG CCTGATACCT
    GATAAGGACC TCATTTCCTG CTCAGAAAAC AATACCTTTG GAAAAGAAGG AGACATATTG
    AAAAAGTCGT GCTCTGTTTT AAATACTACT TTTATCCGAG GCAGCAACAT TTACAAGTGC
    TTCAACAGCT CATGGAAGAT GGAAAAGAAT GACTGCGTAT CCGAACAGAT AAACAACCTG
    CTGCTCATGG CAGAGTCCTT GGTCAACAGT CCCAGTGCAA AAGTTGGTCT ACCTAACTTC
    CTTCAGAACC TGCGGAATCA GACAGAACTG CTGCAAAATG ACAATTCTTC TGCAAATCTT
    GCTGCAATTG TTACCATCCT CTACAACATC TCTTCTATTC CAGTAGAAGC AAAGCAGTCC
    ACTATCTCTG ATTACTTCAC TACAGTGGAC ATTATCGTTA CTGATTTCAA AAAGGAACTT
    TGGGCTGACC TGAATGAAGG GCAGGTACCC ATAAGTTCTT TGTTACTGAA TGCGGTGGAA
    AGATTTTCTG AGAACCTCCA ACCAGTTAAT AATACCATTC CACATCTGTC CACTGAGACC
    CTTGAGCTGC AAGGCATGGT TGTCACAGAA CATAGCCACG CAGACTATAA TAAGAACTTC
    AGCCGTCTAG GAAACCTCAC AGCTAATGTG CTGATTGATA ACATTGAGAC TCTGCCTCCA
    AACTCAACCA TTGTCAGTGT GGCCTACTCA GCACTTGGTC ATATTTTGCC TGTGAAGGAG
    AACATGTCTG TGAACAGTTT AGTGATATCA ACAGCTCTGA GCTGCGAGAG AAACCAGAAC
    TTCCATATTA ATATGACCTT TCAGAAAGAA AACATATCTC TGAAGATGCC CCTGTGTGTC
    TTTTGGGACT TCAAACTCAA CGAAAGTAGG GGAGGCTGGA ATGATCGTGG TTGCACGCAA
    ACAGAGGAAG GCAGCAATGT CATTTGTTCC TGCAATCACT TGACACCATT CTCCATTCTG
    ATGTCACCGG ATGAACCTGC TCACAATTCT GTTGAAGATT ACATCACCCA CATTGGCCTG
    GCCATTTCAA TCTTGAGTTT AGTGGTCTGC ATTGTAATTG AATCCTTAGT TTGGAAAACT
    GTGACAAACA ACACAACCTC CTACATGCGT CACGTCTGTA TTCTCAACAC AGCGACATCT
    CTCCTAATTG CTGACATTTG GTTCATTGTT ACTGCCTCCA TCAATGTGCA GAAAAGAAAT
    ATCTGTATTG CAGCCACATT CTTCATCCAT TTATTCTACC TATGTGGCTT TTTCTGGATG
    CTCAGCCTCG GCCTCATCCT TTTTTACAGA CTAGTCTTCA TCTTACATAA CACAAGCAAG
    ACATCTCAGA AAGCAGTGGT ATTCTGCCTG GGTTATGGGT GCCCCTTTGT GTTTGCAGTC
    ACCACCATTG CTGTCACATT GCCGTGGAAC ACTTACACCA GAAAGGATGC CTGCTGGCTC
    AACTGGAAGG ACAGCAAAGC TGTCTTGGCC TTTGTCATAC CTGCCCTGGC CATCGTGGCC
    ATGAATTTGT TCATCACTGC AGTTGTGATA ATAAAAATAC TAAGGCCGAA CATTGGGGAC
    AGGACAAACA AACAAGAGAG GAAGACCTTC TTTCAAATTT GCAAAAGTCT TGTCATCTTG
    ACACCACTGT TAGGCCTCAC GTGGGGATTT GGTCTTGCCA CCATCATCAG AAATAGCCAC
    CAGACTTTCC ACATCCTCTT TGCTCTGCTC AATGCCTTGC AGGGACTGTT CATTTTGGTA
    TTTGGGACTC TATGGGACAA CAAGATAAAA GAAGCCCTGC TGAAGAGGAA TTCACTCTCC
    AGATGGAGTT CGCAACAAAC AAAGTCAACA TCCCTGATCT TGGTGACACC AATGTTTTCC
    ATGAGCTATC CATTTTCAAG AACATTCAAC AACTTATGTG GCAAAACAGG AAAATACACA
    GTATCTTCTT CAGAGCCATC CAGTGCTTCC ACAGAGAACA TCTCCAAGTC CGATTCTTTG
    CTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07171
    Clone ID Related Accession (Same CDS sequence) XM_004935931.2 , XM_015285166.1 , XM_004935931.3 , XM_015285167.1 , XM_004935933.2 , XM_004935933.3 , XM_015285166.2 , XM_025149283.1 , XM_015285167.2
    Accession Version XM_015285166.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product adhesion G protein-coupled receptor F5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCCATCCC CAATTACTGC AGCTCTCCAC CTCCTGTCTC TACTGGCCGT ATGCTTCCAG 
    ACGTCTCCAG CCTCGGGCTT TGACTCATTC ACCCAATATG CCGTTGGCAG GGAGGTAGGA
    GAGGAGAGCT TCCACCCAGA GCTGCAGAGG CAGAAGCGAC ATGTGATCCT TTTTGACCTT
    CCACCCCTGG AGTATGCCAT TGACATTGAA GTAAGCCTCA CGAATTCATC CTTCCTGGCA
    CCAATCAAGG AGTACTTCAG AAACCTCCAT CTTCCAGTTT CAATCAATAT TTCAGATGTA
    GAAATGACAG TTTCAAGTAT CAACATCACA ACAGTCTGTG TGCCCAGTGA GAACAAGAGC
    TGCTGTTCTT GTGAAAGCGG ATATGCCTGG CCAAGCACGG GGTGCAGTGA CCTGATCAGC
    TGCCCTGCTC TCAGCCGAGC TCCCAACCAG CCATGCGGCT ACACCAGGGA AAGGCCTTTC
    AATGGGCCCT ACTGTGAACC TCAGAATGGA GACTCATGTG GTATGGGAGA GCCCACTGTA
    ATGAATATGT CAGTAAGACT TGACACAGCC TTCCACGATG ATCTCAGGAA TCATTCTTCC
    CAACTATACA GAAAATACAA AGCTGACCTC GATAAAGCGT TTAAAGCTAG CTACGGGTGC
    TTACCGGGCT TTGTGTCAGC AACAACAACT GGTTTCAGGC CTGGGAGTGT TGAGGTGAAC
    TACAGAGTGG AGGCAGGATC AGCGAGCTTC CAACAGCTGG AGCATTCCAG CAGATTTGTT
    ACACAGTACC TGGATGAACC ATACAGCATA GTGCCTACCT CCTTTACAGC AGAAATAACG
    AATCGCACAA ACTTCACAGT GAGCCCTGCC CACATTTTTG AAGGGGATAC CATCCAGATG
    GTGTGTGAGA TAAACACCAC GTCTGAGAAC ATGACCTGGT ATCGTGACAG TCAGATCATC
    CTCAACAGCT CACGGCACTC CGTGAGGACA GACAACAACA CTGCGATGTC ACAGGCATTT
    CTTACAATTA CCAACGTTAC GCAGGAGGAT TCTGGTACCT ACAAATGCAC TTTTGCAAAC
    AGATACCGGT CTTTAAATCT GATATACGAA GGCACCAAAG AAATACAAGT CTCTCCACTA
    CGTATCATCT CATATTCAAA CGACTATAAA GTTCACTGCA ACAGCCCTGA GATACAGGCA
    AACAGCCCTG TGCTGTTCTG TTGCATTGAT GGACAATTAC CACTGCTTAC TGGTGACTGG
    AAGGTGAATG GAGCGATCAG TATTACAGGA GTCCCCAGTT TCAGCAGCAA CTGCACTGAA
    TACAAGCTCA ACATGACGGA CTCGCTGTGC TTGGCCGAGC ATTCAGGTAC GGTGACGACA
    TACACGTGTG AGCTGCAGAC AGGGCACGGC GCCAGGAGCA GCCGGGACAT CCGAGTGATG
    TACTTCCGAG CAGCCCAGGT AAGAATATCT TCCAGCGTAA ACACAACCGT TTCCGTGGGA
    TATGGCTTCA GTCTAAAGTG TGAAAGCGAT ACGAGCAATT ACGACAGCAT CAGCTGGGAA
    ATCCATTCTG GAGATAGAAT ACAAGCAGTA GAATGCGCTG CATGCATTAG AACCAACAAA
    TCCATGGCCA CATCCATGCT CGTGGTGAAC ACTGCCACTC AAGACTGGAA TGGCACCTAC
    ATCTGCACAT TGTCCCAGGA CTCCCTGAAC AGTTCTGCCA GCGTGACAAT CAATGTCATT
    CCGTTGCCCC TGAAGCAGCA CATCCTGATA GACCCCGCTG GAGCGCCTAT CATGTGCGGA
    ACGCCCCAAG CCCTCACCTG CTGCATCAGT GCTAACAGCA TGGGAGATTA TGATGTTAAA
    TTTGTGGTAC AAGAAAAAGA ATTTCAAGCT GAGAAAGAAG AGCAAGGAAA TTTCTTTTGC
    TACTCGTACA ATTACACAGA AACAGAATGC AGGGAAAAGA AGCTGGAGGC GTATTGCAAG
    TTTGTGAACA GCAATGAGCA GGAGGTCAGC AGCAACAGTA TAAATCTGAG CCTGATACCT
    GATAAGGACC TCATTTCCTG CTCAGAAAAC AATACCTTTG GAAAAGAAGG AGACATATTG
    AAAAAGTCGT GCTCTGTTTT AAATACTACT TTTATCCGAG GCAGCAACAT TTACAAGTGC
    TTCAACAGCT CATGGAAGAT GGAAAAGAAT GACTGCGTAT CCGAACAGAT AAACAACCTG
    CTGCTCATGG CAGAGTCCTT GGTCAACAGT CCCAGTGCAA AAGTTGGTCT ACCTAACTTC
    CTTCAGAACC TGCGGAATCA GACAGAACTG CTGCAAAATG ACAATTCTTC TGCAAATCTT
    GCTGCAATTG TTACCATCCT CTACAACATC TCTTCTATTC CAGTAGAAGC AAAGCAGTCC
    ACTATCTCTG ATTACTTCAC TACAGTGGAC ATTATCGTTA CTGATTTCAA AAAGGAACTT
    TGGGCTGACC TGAATGAAGG GCAGGTACCC ATAAGTTCTT TGTTACTGAA TGCGGTGGAA
    AGATTTTCTG AGAACCTCCA ACCAGTTAAT AATACCATTC CACATCTGTC CACTGAGACC
    CTTGAGCTGC AAGGCATGGT TGTCACAGAA CATAGCCACG CAGACTATAA TAAGAACTTC
    AGCCGTCTAG GAAACCTCAC AGCTAATGTG CTGATTGATA ACATTGAGAC TCTGCCTCCA
    AACTCAACCA TTGTCAGTGT GGCCTACTCA GCACTTGGTC ATATTTTGCC TGTGAAGGAG
    AACATGTCTG TGAACAGTTT AGTGATATCA ACAGCTCTGA GCTGCGAGAG AAACCAGAAC
    TTCCATATTA ATATGACCTT TCAGAAAGAA AACATATCTC TGAAGATGCC CCTGTGTGTC
    TTTTGGGACT TCAAACTCAA CGAAAGTAGG GGAGGCTGGA ATGATCGTGG TTGCACGCAA
    ACAGAGGAAG GCAGCAATGT CATTTGTTCC TGCAATCACT TGACACCATT CTCCATTCTG
    ATGTCACCGG ATGAACCTGC TCACAATTCT GTTGAAGATT ACATCACCCA CATTGGCCTG
    GCCATTTCAA TCTTGAGTTT AGTGGTCTGC ATTGTAATTG AATCCTTAGT TTGGAAAACT
    GTGACAAACA ACACAACCTC CTACATGCGT CACGTCTGTA TTCTCAACAC AGCGACATCT
    CTCCTAATTG CTGACATTTG GTTCATTGTT ACTGCCTCCA TCAATGTGCA GAAAAGAAAT
    ATCTGTATTG CAGCCACATT CTTCATCCAT TTATTCTACC TATGTGGCTT TTTCTGGATG
    CTCAGCCTCG GCCTCATCCT TTTTTACAGA CTAGTCTTCA TCTTACATAA CACAAGCAAG
    ACATCTCAGA AAGCAGTGGT ATTCTGCCTG GGTTATGGGT GCCCCTTTGT GTTTGCAGTC
    ACCACCATTG CTGTCACATT GCCGTGGAAC ACTTACACCA GAAAGGATGC CTGCTGGCTC
    AACTGGAAGG ACAGCAAAGC TGTCTTGGCC TTTGTCATAC CTGCCCTGGC CATCGTGGCC
    ATGAATTTGT TCATCACTGC AGTTGTGATA ATAAAAATAC TAAGGCCGAA CATTGGGGAC
    AGGACAAACA AACAAGAGAG GAAGACCTTC TTTCAAATTT GCAAAAGTCT TGTCATCTTG
    ACACCACTGT TAGGCCTCAC GTGGGGATTT GGTCTTGCCA CCATCATCAG AAATAGCCAC
    CAGACTTTCC ACATCCTCTT TGCTCTGCTC AATGCCTTGC AGGGACTGTT CATTTTGGTA
    TTTGGGACTC TATGGGACAA CAAGATAAAA GAAGCCCTGC TGAAGAGGAA TTCACTCTCC
    AGATGGAGTT CGCAACAAAC AAAGTCAACA TCCCTGATCT TGGTGACACC AATGTTTTCC
    ATGAGCTATC CATTTTCAAG AACATTCAAC AACTTATGTG GCAAAACAGG AAAATACACA
    GTATCTTCTT CAGAGCCATC CAGTGCTTCC ACAGAGAACA TCTCCAAGTC CGATTCTTTG
    CTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140652.1
    CDS448..4476
    Translation

    Target ORF information:

    RefSeq Version XM_015285166.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285166.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCCATCCC CAATTACTGC AGCTCTCCAC CTCCTGTCTC TACTGGCCGT ATGCTTCCAG 
    ACGTCTCCAG CCTCGGGCTT TGACTCATTC ACCCAATATG CCGTTGGCAG GGAGGTAGGA
    GAGGAGAGCT TCCACCCAGA GCTGCAGAGG CAGAAGCGAC ATGTGATCCT TTTTGACCTT
    CCACCCCTGG AGTATGCCAT TGACATTGAA GTAAGCCTCA CGAATTCATC CTTCCTGGCA
    CCAATCAAGG AGTACTTCAG AAACCTCCAT CTTCCAGTTT CAATCAATAT TTCAGATGTA
    GAAATGACAG TTTCAAGTAT CAACATCACA ACAGTCTGTG TGCCCAGTGA GAACAAGAGC
    TGCTGTTCTT GTGAAAGCGG ATATGCCTGG CCAAGCACGG GGTGCAGTGA CCTGATCAGC
    TGCCCTGCTC TCAGCCGAGC TCCCAACCAG CCATGCGGCT ACACCAGGGA AAGGCCTTTC
    AATGGGCCCT ACTGTGAACC TCAGAATGGA GACTCATGTG GTATGGGAGA GCCCACTGTA
    ATGAATATGT CAGTAAGACT TGACACAGCC TTCCACGATG ATCTCAGGAA TCATTCTTCC
    CAACTATACA GAAAATACAA AGCTGACCTC GATAAAGCGT TTAAAGCTAG CTACGGGTGC
    TTACCGGGCT TTGTGTCAGC AACAACAACT GGTTTCAGGC CTGGGAGTGT TGAGGTGAAC
    TACAGAGTGG AGGCAGGATC AGCGAGCTTC CAACAGCTGG AGCATTCCAG CAGATTTGTT
    ACACAGTACC TGGATGAACC ATACAGCATA GTGCCTACCT CCTTTACAGC AGAAATAACG
    AATCGCACAA ACTTCACAGT GAGCCCTGCC CACATTTTTG AAGGGGATAC CATCCAGATG
    GTGTGTGAGA TAAACACCAC GTCTGAGAAC ATGACCTGGT ATCGTGACAG TCAGATCATC
    CTCAACAGCT CACGGCACTC CGTGAGGACA GACAACAACA CTGCGATGTC ACAGGCATTT
    CTTACAATTA CCAACGTTAC GCAGGAGGAT TCTGGTACCT ACAAATGCAC TTTTGCAAAC
    AGATACCGGT CTTTAAATCT GATATACGAA GGCACCAAAG AAATACAAGT CTCTCCACTA
    CGTATCATCT CATATTCAAA CGACTATAAA GTTCACTGCA ACAGCCCTGA GATACAGGCA
    AACAGCCCTG TGCTGTTCTG TTGCATTGAT GGACAATTAC CACTGCTTAC TGGTGACTGG
    AAGGTGAATG GAGCGATCAG TATTACAGGA GTCCCCAGTT TCAGCAGCAA CTGCACTGAA
    TACAAGCTCA ACATGACGGA CTCGCTGTGC TTGGCCGAGC ATTCAGGTAC GGTGACGACA
    TACACGTGTG AGCTGCAGAC AGGGCACGGC GCCAGGAGCA GCCGGGACAT CCGAGTGATG
    TACTTCCGAG CAGCCCAGGT AAGAATATCT TCCAGCGTAA ACACAACCGT TTCCGTGGGA
    TATGGCTTCA GTCTAAAGTG TGAAAGCGAT ACGAGCAATT ACGACAGCAT CAGCTGGGAA
    ATCCATTCTG GAGATAGAAT ACAAGCAGTA GAATGCGCTG CATGCATTAG AACCAACAAA
    TCCATGGCCA CATCCATGCT CGTGGTGAAC ACTGCCACTC AAGACTGGAA TGGCACCTAC
    ATCTGCACAT TGTCCCAGGA CTCCCTGAAC AGTTCTGCCA GCGTGACAAT CAATGTCATT
    CCGTTGCCCC TGAAGCAGCA CATCCTGATA GACCCCGCTG GAGCGCCTAT CATGTGCGGA
    ACGCCCCAAG CCCTCACCTG CTGCATCAGT GCTAACAGCA TGGGAGATTA TGATGTTAAA
    TTTGTGGTAC AAGAAAAAGA ATTTCAAGCT GAGAAAGAAG AGCAAGGAAA TTTCTTTTGC
    TACTCGTACA ATTACACAGA AACAGAATGC AGGGAAAAGA AGCTGGAGGC GTATTGCAAG
    TTTGTGAACA GCAATGAGCA GGAGGTCAGC AGCAACAGTA TAAATCTGAG CCTGATACCT
    GATAAGGACC TCATTTCCTG CTCAGAAAAC AATACCTTTG GAAAAGAAGG AGACATATTG
    AAAAAGTCGT GCTCTGTTTT AAATACTACT TTTATCCGAG GCAGCAACAT TTACAAGTGC
    TTCAACAGCT CATGGAAGAT GGAAAAGAAT GACTGCGTAT CCGAACAGAT AAACAACCTG
    CTGCTCATGG CAGAGTCCTT GGTCAACAGT CCCAGTGCAA AAGTTGGTCT ACCTAACTTC
    CTTCAGAACC TGCGGAATCA GACAGAACTG CTGCAAAATG ACAATTCTTC TGCAAATCTT
    GCTGCAATTG TTACCATCCT CTACAACATC TCTTCTATTC CAGTAGAAGC AAAGCAGTCC
    ACTATCTCTG ATTACTTCAC TACAGTGGAC ATTATCGTTA CTGATTTCAA AAAGGAACTT
    TGGGCTGACC TGAATGAAGG GCAGGTACCC ATAAGTTCTT TGTTACTGAA TGCGGTGGAA
    AGATTTTCTG AGAACCTCCA ACCAGTTAAT AATACCATTC CACATCTGTC CACTGAGACC
    CTTGAGCTGC AAGGCATGGT TGTCACAGAA CATAGCCACG CAGACTATAA TAAGAACTTC
    AGCCGTCTAG GAAACCTCAC AGCTAATGTG CTGATTGATA ACATTGAGAC TCTGCCTCCA
    AACTCAACCA TTGTCAGTGT GGCCTACTCA GCACTTGGTC ATATTTTGCC TGTGAAGGAG
    AACATGTCTG TGAACAGTTT AGTGATATCA ACAGCTCTGA GCTGCGAGAG AAACCAGAAC
    TTCCATATTA ATATGACCTT TCAGAAAGAA AACATATCTC TGAAGATGCC CCTGTGTGTC
    TTTTGGGACT TCAAACTCAA CGAAAGTAGG GGAGGCTGGA ATGATCGTGG TTGCACGCAA
    ACAGAGGAAG GCAGCAATGT CATTTGTTCC TGCAATCACT TGACACCATT CTCCATTCTG
    ATGTCACCGG ATGAACCTGC TCACAATTCT GTTGAAGATT ACATCACCCA CATTGGCCTG
    GCCATTTCAA TCTTGAGTTT AGTGGTCTGC ATTGTAATTG AATCCTTAGT TTGGAAAACT
    GTGACAAACA ACACAACCTC CTACATGCGT CACGTCTGTA TTCTCAACAC AGCGACATCT
    CTCCTAATTG CTGACATTTG GTTCATTGTT ACTGCCTCCA TCAATGTGCA GAAAAGAAAT
    ATCTGTATTG CAGCCACATT CTTCATCCAT TTATTCTACC TATGTGGCTT TTTCTGGATG
    CTCAGCCTCG GCCTCATCCT TTTTTACAGA CTAGTCTTCA TCTTACATAA CACAAGCAAG
    ACATCTCAGA AAGCAGTGGT ATTCTGCCTG GGTTATGGGT GCCCCTTTGT GTTTGCAGTC
    ACCACCATTG CTGTCACATT GCCGTGGAAC ACTTACACCA GAAAGGATGC CTGCTGGCTC
    AACTGGAAGG ACAGCAAAGC TGTCTTGGCC TTTGTCATAC CTGCCCTGGC CATCGTGGCC
    ATGAATTTGT TCATCACTGC AGTTGTGATA ATAAAAATAC TAAGGCCGAA CATTGGGGAC
    AGGACAAACA AACAAGAGAG GAAGACCTTC TTTCAAATTT GCAAAAGTCT TGTCATCTTG
    ACACCACTGT TAGGCCTCAC GTGGGGATTT GGTCTTGCCA CCATCATCAG AAATAGCCAC
    CAGACTTTCC ACATCCTCTT TGCTCTGCTC AATGCCTTGC AGGGACTGTT CATTTTGGTA
    TTTGGGACTC TATGGGACAA CAAGATAAAA GAAGCCCTGC TGAAGAGGAA TTCACTCTCC
    AGATGGAGTT CGCAACAAAC AAAGTCAACA TCCCTGATCT TGGTGACACC AATGTTTTCC
    ATGAGCTATC CATTTTCAAG AACATTCAAC AACTTATGTG GCAAAACAGG AAAATACACA
    GTATCTTCTT CAGAGCCATC CAGTGCTTCC ACAGAGAACA TCTCCAAGTC CGATTCTTTG
    CTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07171
    Clone ID Related Accession (Same CDS sequence) XM_004935931.2 , XM_015285166.1 , XM_004935931.3 , XM_015285167.1 , XM_004935933.2 , XM_004935933.3 , XM_015285166.2 , XM_025149283.1 , XM_015285167.2
    Accession Version XM_004935931.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product adhesion G protein-coupled receptor F5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004935931.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCCATCCC CAATTACTGC AGCTCTCCAC CTCCTGTCTC TACTGGCCGT ATGCTTCCAG 
    ACGTCTCCAG CCTCGGGCTT TGACTCATTC ACCCAATATG CCGTTGGCAG GGAGGTAGGA
    GAGGAGAGCT TCCACCCAGA GCTGCAGAGG CAGAAGCGAC ATGTGATCCT TTTTGACCTT
    CCACCCCTGG AGTATGCCAT TGACATTGAA GTAAGCCTCA CGAATTCATC CTTCCTGGCA
    CCAATCAAGG AGTACTTCAG AAACCTCCAT CTTCCAGTTT CAATCAATAT TTCAGATGTA
    GAAATGACAG TTTCAAGTAT CAACATCACA ACAGTCTGTG TGCCCAGTGA GAACAAGAGC
    TGCTGTTCTT GTGAAAGCGG ATATGCCTGG CCAAGCACGG GGTGCAGTGA CCTGATCAGC
    TGCCCTGCTC TCAGCCGAGC TCCCAACCAG CCATGCGGCT ACACCAGGGA AAGGCCTTTC
    AATGGGCCCT ACTGTGAACC TCAGAATGGA GACTCATGTG GTATGGGAGA GCCCACTGTA
    ATGAATATGT CAGTAAGACT TGACACAGCC TTCCACGATG ATCTCAGGAA TCATTCTTCC
    CAACTATACA GAAAATACAA AGCTGACCTC GATAAAGCGT TTAAAGCTAG CTACGGGTGC
    TTACCGGGCT TTGTGTCAGC AACAACAACT GGTTTCAGGC CTGGGAGTGT TGAGGTGAAC
    TACAGAGTGG AGGCAGGATC AGCGAGCTTC CAACAGCTGG AGCATTCCAG CAGATTTGTT
    ACACAGTACC TGGATGAACC ATACAGCATA GTGCCTACCT CCTTTACAGC AGAAATAACG
    AATCGCACAA ACTTCACAGT GAGCCCTGCC CACATTTTTG AAGGGGATAC CATCCAGATG
    GTGTGTGAGA TAAACACCAC GTCTGAGAAC ATGACCTGGT ATCGTGACAG TCAGATCATC
    CTCAACAGCT CACGGCACTC CGTGAGGACA GACAACAACA CTGCGATGTC ACAGGCATTT
    CTTACAATTA CCAACGTTAC GCAGGAGGAT TCTGGTACCT ACAAATGCAC TTTTGCAAAC
    AGATACCGGT CTTTAAATCT GATATACGAA GGCACCAAAG AAATACAAGT CTCTCCACTA
    CGTATCATCT CATATTCAAA CGACTATAAA GTTCACTGCA ACAGCCCTGA GATACAGGCA
    AACAGCCCTG TGCTGTTCTG TTGCATTGAT GGACAATTAC CACTGCTTAC TGGTGACTGG
    AAGGTGAATG GAGCGATCAG TATTACAGGA GTCCCCAGTT TCAGCAGCAA CTGCACTGAA
    TACAAGCTCA ACATGACGGA CTCGCTGTGC TTGGCCGAGC ATTCAGGTAC GGTGACGACA
    TACACGTGTG AGCTGCAGAC AGGGCACGGC GCCAGGAGCA GCCGGGACAT CCGAGTGATG
    TACTTCCGAG CAGCCCAGGT AAGAATATCT TCCAGCGTAA ACACAACCGT TTCCGTGGGA
    TATGGCTTCA GTCTAAAGTG TGAAAGCGAT ACGAGCAATT ACGACAGCAT CAGCTGGGAA
    ATCCATTCTG GAGATAGAAT ACAAGCAGTA GAATGCGCTG CATGCATTAG AACCAACAAA
    TCCATGGCCA CATCCATGCT CGTGGTGAAC ACTGCCACTC AAGACTGGAA TGGCACCTAC
    ATCTGCACAT TGTCCCAGGA CTCCCTGAAC AGTTCTGCCA GCGTGACAAT CAATGTCATT
    CCGTTGCCCC TGAAGCAGCA CATCCTGATA GACCCCGCTG GAGCGCCTAT CATGTGCGGA
    ACGCCCCAAG CCCTCACCTG CTGCATCAGT GCTAACAGCA TGGGAGATTA TGATGTTAAA
    TTTGTGGTAC AAGAAAAAGA ATTTCAAGCT GAGAAAGAAG AGCAAGGAAA TTTCTTTTGC
    TACTCGTACA ATTACACAGA AACAGAATGC AGGGAAAAGA AGCTGGAGGC GTATTGCAAG
    TTTGTGAACA GCAATGAGCA GGAGGTCAGC AGCAACAGTA TAAATCTGAG CCTGATACCT
    GATAAGGACC TCATTTCCTG CTCAGAAAAC AATACCTTTG GAAAAGAAGG AGACATATTG
    AAAAAGTCGT GCTCTGTTTT AAATACTACT TTTATCCGAG GCAGCAACAT TTACAAGTGC
    TTCAACAGCT CATGGAAGAT GGAAAAGAAT GACTGCGTAT CCGAACAGAT AAACAACCTG
    CTGCTCATGG CAGAGTCCTT GGTCAACAGT CCCAGTGCAA AAGTTGGTCT ACCTAACTTC
    CTTCAGAACC TGCGGAATCA GACAGAACTG CTGCAAAATG ACAATTCTTC TGCAAATCTT
    GCTGCAATTG TTACCATCCT CTACAACATC TCTTCTATTC CAGTAGAAGC AAAGCAGTCC
    ACTATCTCTG ATTACTTCAC TACAGTGGAC ATTATCGTTA CTGATTTCAA AAAGGAACTT
    TGGGCTGACC TGAATGAAGG GCAGGTACCC ATAAGTTCTT TGTTACTGAA TGCGGTGGAA
    AGATTTTCTG AGAACCTCCA ACCAGTTAAT AATACCATTC CACATCTGTC CACTGAGACC
    CTTGAGCTGC AAGGCATGGT TGTCACAGAA CATAGCCACG CAGACTATAA TAAGAACTTC
    AGCCGTCTAG GAAACCTCAC AGCTAATGTG CTGATTGATA ACATTGAGAC TCTGCCTCCA
    AACTCAACCA TTGTCAGTGT GGCCTACTCA GCACTTGGTC ATATTTTGCC TGTGAAGGAG
    AACATGTCTG TGAACAGTTT AGTGATATCA ACAGCTCTGA GCTGCGAGAG AAACCAGAAC
    TTCCATATTA ATATGACCTT TCAGAAAGAA AACATATCTC TGAAGATGCC CCTGTGTGTC
    TTTTGGGACT TCAAACTCAA CGAAAGTAGG GGAGGCTGGA ATGATCGTGG TTGCACGCAA
    ACAGAGGAAG GCAGCAATGT CATTTGTTCC TGCAATCACT TGACACCATT CTCCATTCTG
    ATGTCACCGG ATGAACCTGC TCACAATTCT GTTGAAGATT ACATCACCCA CATTGGCCTG
    GCCATTTCAA TCTTGAGTTT AGTGGTCTGC ATTGTAATTG AATCCTTAGT TTGGAAAACT
    GTGACAAACA ACACAACCTC CTACATGCGT CACGTCTGTA TTCTCAACAC AGCGACATCT
    CTCCTAATTG CTGACATTTG GTTCATTGTT ACTGCCTCCA TCAATGTGCA GAAAAGAAAT
    ATCTGTATTG CAGCCACATT CTTCATCCAT TTATTCTACC TATGTGGCTT TTTCTGGATG
    CTCAGCCTCG GCCTCATCCT TTTTTACAGA CTAGTCTTCA TCTTACATAA CACAAGCAAG
    ACATCTCAGA AAGCAGTGGT ATTCTGCCTG GGTTATGGGT GCCCCTTTGT GTTTGCAGTC
    ACCACCATTG CTGTCACATT GCCGTGGAAC ACTTACACCA GAAAGGATGC CTGCTGGCTC
    AACTGGAAGG ACAGCAAAGC TGTCTTGGCC TTTGTCATAC CTGCCCTGGC CATCGTGGCC
    ATGAATTTGT TCATCACTGC AGTTGTGATA ATAAAAATAC TAAGGCCGAA CATTGGGGAC
    AGGACAAACA AACAAGAGAG GAAGACCTTC TTTCAAATTT GCAAAAGTCT TGTCATCTTG
    ACACCACTGT TAGGCCTCAC GTGGGGATTT GGTCTTGCCA CCATCATCAG AAATAGCCAC
    CAGACTTTCC ACATCCTCTT TGCTCTGCTC AATGCCTTGC AGGGACTGTT CATTTTGGTA
    TTTGGGACTC TATGGGACAA CAAGATAAAA GAAGCCCTGC TGAAGAGGAA TTCACTCTCC
    AGATGGAGTT CGCAACAAAC AAAGTCAACA TCCCTGATCT TGGTGACACC AATGTTTTCC
    ATGAGCTATC CATTTTCAAG AACATTCAAC AACTTATGTG GCAAAACAGG AAAATACACA
    GTATCTTCTT CAGAGCCATC CAGTGCTTCC ACAGAGAACA TCTCCAAGTC CGATTCTTTG
    CTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004935988.2
    CDS437..4465
    Translation

    Target ORF information:

    RefSeq Version XM_004935931.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004935931.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCCATCCC CAATTACTGC AGCTCTCCAC CTCCTGTCTC TACTGGCCGT ATGCTTCCAG 
    ACGTCTCCAG CCTCGGGCTT TGACTCATTC ACCCAATATG CCGTTGGCAG GGAGGTAGGA
    GAGGAGAGCT TCCACCCAGA GCTGCAGAGG CAGAAGCGAC ATGTGATCCT TTTTGACCTT
    CCACCCCTGG AGTATGCCAT TGACATTGAA GTAAGCCTCA CGAATTCATC CTTCCTGGCA
    CCAATCAAGG AGTACTTCAG AAACCTCCAT CTTCCAGTTT CAATCAATAT TTCAGATGTA
    GAAATGACAG TTTCAAGTAT CAACATCACA ACAGTCTGTG TGCCCAGTGA GAACAAGAGC
    TGCTGTTCTT GTGAAAGCGG ATATGCCTGG CCAAGCACGG GGTGCAGTGA CCTGATCAGC
    TGCCCTGCTC TCAGCCGAGC TCCCAACCAG CCATGCGGCT ACACCAGGGA AAGGCCTTTC
    AATGGGCCCT ACTGTGAACC TCAGAATGGA GACTCATGTG GTATGGGAGA GCCCACTGTA
    ATGAATATGT CAGTAAGACT TGACACAGCC TTCCACGATG ATCTCAGGAA TCATTCTTCC
    CAACTATACA GAAAATACAA AGCTGACCTC GATAAAGCGT TTAAAGCTAG CTACGGGTGC
    TTACCGGGCT TTGTGTCAGC AACAACAACT GGTTTCAGGC CTGGGAGTGT TGAGGTGAAC
    TACAGAGTGG AGGCAGGATC AGCGAGCTTC CAACAGCTGG AGCATTCCAG CAGATTTGTT
    ACACAGTACC TGGATGAACC ATACAGCATA GTGCCTACCT CCTTTACAGC AGAAATAACG
    AATCGCACAA ACTTCACAGT GAGCCCTGCC CACATTTTTG AAGGGGATAC CATCCAGATG
    GTGTGTGAGA TAAACACCAC GTCTGAGAAC ATGACCTGGT ATCGTGACAG TCAGATCATC
    CTCAACAGCT CACGGCACTC CGTGAGGACA GACAACAACA CTGCGATGTC ACAGGCATTT
    CTTACAATTA CCAACGTTAC GCAGGAGGAT TCTGGTACCT ACAAATGCAC TTTTGCAAAC
    AGATACCGGT CTTTAAATCT GATATACGAA GGCACCAAAG AAATACAAGT CTCTCCACTA
    CGTATCATCT CATATTCAAA CGACTATAAA GTTCACTGCA ACAGCCCTGA GATACAGGCA
    AACAGCCCTG TGCTGTTCTG TTGCATTGAT GGACAATTAC CACTGCTTAC TGGTGACTGG
    AAGGTGAATG GAGCGATCAG TATTACAGGA GTCCCCAGTT TCAGCAGCAA CTGCACTGAA
    TACAAGCTCA ACATGACGGA CTCGCTGTGC TTGGCCGAGC ATTCAGGTAC GGTGACGACA
    TACACGTGTG AGCTGCAGAC AGGGCACGGC GCCAGGAGCA GCCGGGACAT CCGAGTGATG
    TACTTCCGAG CAGCCCAGGT AAGAATATCT TCCAGCGTAA ACACAACCGT TTCCGTGGGA
    TATGGCTTCA GTCTAAAGTG TGAAAGCGAT ACGAGCAATT ACGACAGCAT CAGCTGGGAA
    ATCCATTCTG GAGATAGAAT ACAAGCAGTA GAATGCGCTG CATGCATTAG AACCAACAAA
    TCCATGGCCA CATCCATGCT CGTGGTGAAC ACTGCCACTC AAGACTGGAA TGGCACCTAC
    ATCTGCACAT TGTCCCAGGA CTCCCTGAAC AGTTCTGCCA GCGTGACAAT CAATGTCATT
    CCGTTGCCCC TGAAGCAGCA CATCCTGATA GACCCCGCTG GAGCGCCTAT CATGTGCGGA
    ACGCCCCAAG CCCTCACCTG CTGCATCAGT GCTAACAGCA TGGGAGATTA TGATGTTAAA
    TTTGTGGTAC AAGAAAAAGA ATTTCAAGCT GAGAAAGAAG AGCAAGGAAA TTTCTTTTGC
    TACTCGTACA ATTACACAGA AACAGAATGC AGGGAAAAGA AGCTGGAGGC GTATTGCAAG
    TTTGTGAACA GCAATGAGCA GGAGGTCAGC AGCAACAGTA TAAATCTGAG CCTGATACCT
    GATAAGGACC TCATTTCCTG CTCAGAAAAC AATACCTTTG GAAAAGAAGG AGACATATTG
    AAAAAGTCGT GCTCTGTTTT AAATACTACT TTTATCCGAG GCAGCAACAT TTACAAGTGC
    TTCAACAGCT CATGGAAGAT GGAAAAGAAT GACTGCGTAT CCGAACAGAT AAACAACCTG
    CTGCTCATGG CAGAGTCCTT GGTCAACAGT CCCAGTGCAA AAGTTGGTCT ACCTAACTTC
    CTTCAGAACC TGCGGAATCA GACAGAACTG CTGCAAAATG ACAATTCTTC TGCAAATCTT
    GCTGCAATTG TTACCATCCT CTACAACATC TCTTCTATTC CAGTAGAAGC AAAGCAGTCC
    ACTATCTCTG ATTACTTCAC TACAGTGGAC ATTATCGTTA CTGATTTCAA AAAGGAACTT
    TGGGCTGACC TGAATGAAGG GCAGGTACCC ATAAGTTCTT TGTTACTGAA TGCGGTGGAA
    AGATTTTCTG AGAACCTCCA ACCAGTTAAT AATACCATTC CACATCTGTC CACTGAGACC
    CTTGAGCTGC AAGGCATGGT TGTCACAGAA CATAGCCACG CAGACTATAA TAAGAACTTC
    AGCCGTCTAG GAAACCTCAC AGCTAATGTG CTGATTGATA ACATTGAGAC TCTGCCTCCA
    AACTCAACCA TTGTCAGTGT GGCCTACTCA GCACTTGGTC ATATTTTGCC TGTGAAGGAG
    AACATGTCTG TGAACAGTTT AGTGATATCA ACAGCTCTGA GCTGCGAGAG AAACCAGAAC
    TTCCATATTA ATATGACCTT TCAGAAAGAA AACATATCTC TGAAGATGCC CCTGTGTGTC
    TTTTGGGACT TCAAACTCAA CGAAAGTAGG GGAGGCTGGA ATGATCGTGG TTGCACGCAA
    ACAGAGGAAG GCAGCAATGT CATTTGTTCC TGCAATCACT TGACACCATT CTCCATTCTG
    ATGTCACCGG ATGAACCTGC TCACAATTCT GTTGAAGATT ACATCACCCA CATTGGCCTG
    GCCATTTCAA TCTTGAGTTT AGTGGTCTGC ATTGTAATTG AATCCTTAGT TTGGAAAACT
    GTGACAAACA ACACAACCTC CTACATGCGT CACGTCTGTA TTCTCAACAC AGCGACATCT
    CTCCTAATTG CTGACATTTG GTTCATTGTT ACTGCCTCCA TCAATGTGCA GAAAAGAAAT
    ATCTGTATTG CAGCCACATT CTTCATCCAT TTATTCTACC TATGTGGCTT TTTCTGGATG
    CTCAGCCTCG GCCTCATCCT TTTTTACAGA CTAGTCTTCA TCTTACATAA CACAAGCAAG
    ACATCTCAGA AAGCAGTGGT ATTCTGCCTG GGTTATGGGT GCCCCTTTGT GTTTGCAGTC
    ACCACCATTG CTGTCACATT GCCGTGGAAC ACTTACACCA GAAAGGATGC CTGCTGGCTC
    AACTGGAAGG ACAGCAAAGC TGTCTTGGCC TTTGTCATAC CTGCCCTGGC CATCGTGGCC
    ATGAATTTGT TCATCACTGC AGTTGTGATA ATAAAAATAC TAAGGCCGAA CATTGGGGAC
    AGGACAAACA AACAAGAGAG GAAGACCTTC TTTCAAATTT GCAAAAGTCT TGTCATCTTG
    ACACCACTGT TAGGCCTCAC GTGGGGATTT GGTCTTGCCA CCATCATCAG AAATAGCCAC
    CAGACTTTCC ACATCCTCTT TGCTCTGCTC AATGCCTTGC AGGGACTGTT CATTTTGGTA
    TTTGGGACTC TATGGGACAA CAAGATAAAA GAAGCCCTGC TGAAGAGGAA TTCACTCTCC
    AGATGGAGTT CGCAACAAAC AAAGTCAACA TCCCTGATCT TGGTGACACC AATGTTTTCC
    ATGAGCTATC CATTTTCAAG AACATTCAAC AACTTATGTG GCAAAACAGG AAAATACACA
    GTATCTTCTT CAGAGCCATC CAGTGCTTCC ACAGAGAACA TCTCCAAGTC CGATTCTTTG
    CTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07171
    Clone ID Related Accession (Same CDS sequence) XM_004935931.2 , XM_015285166.1 , XM_004935931.3 , XM_015285167.1 , XM_004935933.2 , XM_004935933.3 , XM_015285166.2 , XM_025149283.1 , XM_015285167.2
    Accession Version XM_015285167.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product adhesion G protein-coupled receptor F5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCCATCCC CAATTACTGC AGCTCTCCAC CTCCTGTCTC TACTGGCCGT ATGCTTCCAG 
    ACGTCTCCAG CCTCGGGCTT TGACTCATTC ACCCAATATG CCGTTGGCAG GGAGGTAGGA
    GAGGAGAGCT TCCACCCAGA GCTGCAGAGG CAGAAGCGAC ATGTGATCCT TTTTGACCTT
    CCACCCCTGG AGTATGCCAT TGACATTGAA GTAAGCCTCA CGAATTCATC CTTCCTGGCA
    CCAATCAAGG AGTACTTCAG AAACCTCCAT CTTCCAGTTT CAATCAATAT TTCAGATGTA
    GAAATGACAG TTTCAAGTAT CAACATCACA ACAGTCTGTG TGCCCAGTGA GAACAAGAGC
    TGCTGTTCTT GTGAAAGCGG ATATGCCTGG CCAAGCACGG GGTGCAGTGA CCTGATCAGC
    TGCCCTGCTC TCAGCCGAGC TCCCAACCAG CCATGCGGCT ACACCAGGGA AAGGCCTTTC
    AATGGGCCCT ACTGTGAACC TCAGAATGGA GACTCATGTG GTATGGGAGA GCCCACTGTA
    ATGAATATGT CAGTAAGACT TGACACAGCC TTCCACGATG ATCTCAGGAA TCATTCTTCC
    CAACTATACA GAAAATACAA AGCTGACCTC GATAAAGCGT TTAAAGCTAG CTACGGGTGC
    TTACCGGGCT TTGTGTCAGC AACAACAACT GGTTTCAGGC CTGGGAGTGT TGAGGTGAAC
    TACAGAGTGG AGGCAGGATC AGCGAGCTTC CAACAGCTGG AGCATTCCAG CAGATTTGTT
    ACACAGTACC TGGATGAACC ATACAGCATA GTGCCTACCT CCTTTACAGC AGAAATAACG
    AATCGCACAA ACTTCACAGT GAGCCCTGCC CACATTTTTG AAGGGGATAC CATCCAGATG
    GTGTGTGAGA TAAACACCAC GTCTGAGAAC ATGACCTGGT ATCGTGACAG TCAGATCATC
    CTCAACAGCT CACGGCACTC CGTGAGGACA GACAACAACA CTGCGATGTC ACAGGCATTT
    CTTACAATTA CCAACGTTAC GCAGGAGGAT TCTGGTACCT ACAAATGCAC TTTTGCAAAC
    AGATACCGGT CTTTAAATCT GATATACGAA GGCACCAAAG AAATACAAGT CTCTCCACTA
    CGTATCATCT CATATTCAAA CGACTATAAA GTTCACTGCA ACAGCCCTGA GATACAGGCA
    AACAGCCCTG TGCTGTTCTG TTGCATTGAT GGACAATTAC CACTGCTTAC TGGTGACTGG
    AAGGTGAATG GAGCGATCAG TATTACAGGA GTCCCCAGTT TCAGCAGCAA CTGCACTGAA
    TACAAGCTCA ACATGACGGA CTCGCTGTGC TTGGCCGAGC ATTCAGGTAC GGTGACGACA
    TACACGTGTG AGCTGCAGAC AGGGCACGGC GCCAGGAGCA GCCGGGACAT CCGAGTGATG
    TACTTCCGAG CAGCCCAGGT AAGAATATCT TCCAGCGTAA ACACAACCGT TTCCGTGGGA
    TATGGCTTCA GTCTAAAGTG TGAAAGCGAT ACGAGCAATT ACGACAGCAT CAGCTGGGAA
    ATCCATTCTG GAGATAGAAT ACAAGCAGTA GAATGCGCTG CATGCATTAG AACCAACAAA
    TCCATGGCCA CATCCATGCT CGTGGTGAAC ACTGCCACTC AAGACTGGAA TGGCACCTAC
    ATCTGCACAT TGTCCCAGGA CTCCCTGAAC AGTTCTGCCA GCGTGACAAT CAATGTCATT
    CCGTTGCCCC TGAAGCAGCA CATCCTGATA GACCCCGCTG GAGCGCCTAT CATGTGCGGA
    ACGCCCCAAG CCCTCACCTG CTGCATCAGT GCTAACAGCA TGGGAGATTA TGATGTTAAA
    TTTGTGGTAC AAGAAAAAGA ATTTCAAGCT GAGAAAGAAG AGCAAGGAAA TTTCTTTTGC
    TACTCGTACA ATTACACAGA AACAGAATGC AGGGAAAAGA AGCTGGAGGC GTATTGCAAG
    TTTGTGAACA GCAATGAGCA GGAGGTCAGC AGCAACAGTA TAAATCTGAG CCTGATACCT
    GATAAGGACC TCATTTCCTG CTCAGAAAAC AATACCTTTG GAAAAGAAGG AGACATATTG
    AAAAAGTCGT GCTCTGTTTT AAATACTACT TTTATCCGAG GCAGCAACAT TTACAAGTGC
    TTCAACAGCT CATGGAAGAT GGAAAAGAAT GACTGCGTAT CCGAACAGAT AAACAACCTG
    CTGCTCATGG CAGAGTCCTT GGTCAACAGT CCCAGTGCAA AAGTTGGTCT ACCTAACTTC
    CTTCAGAACC TGCGGAATCA GACAGAACTG CTGCAAAATG ACAATTCTTC TGCAAATCTT
    GCTGCAATTG TTACCATCCT CTACAACATC TCTTCTATTC CAGTAGAAGC AAAGCAGTCC
    ACTATCTCTG ATTACTTCAC TACAGTGGAC ATTATCGTTA CTGATTTCAA AAAGGAACTT
    TGGGCTGACC TGAATGAAGG GCAGGTACCC ATAAGTTCTT TGTTACTGAA TGCGGTGGAA
    AGATTTTCTG AGAACCTCCA ACCAGTTAAT AATACCATTC CACATCTGTC CACTGAGACC
    CTTGAGCTGC AAGGCATGGT TGTCACAGAA CATAGCCACG CAGACTATAA TAAGAACTTC
    AGCCGTCTAG GAAACCTCAC AGCTAATGTG CTGATTGATA ACATTGAGAC TCTGCCTCCA
    AACTCAACCA TTGTCAGTGT GGCCTACTCA GCACTTGGTC ATATTTTGCC TGTGAAGGAG
    AACATGTCTG TGAACAGTTT AGTGATATCA ACAGCTCTGA GCTGCGAGAG AAACCAGAAC
    TTCCATATTA ATATGACCTT TCAGAAAGAA AACATATCTC TGAAGATGCC CCTGTGTGTC
    TTTTGGGACT TCAAACTCAA CGAAAGTAGG GGAGGCTGGA ATGATCGTGG TTGCACGCAA
    ACAGAGGAAG GCAGCAATGT CATTTGTTCC TGCAATCACT TGACACCATT CTCCATTCTG
    ATGTCACCGG ATGAACCTGC TCACAATTCT GTTGAAGATT ACATCACCCA CATTGGCCTG
    GCCATTTCAA TCTTGAGTTT AGTGGTCTGC ATTGTAATTG AATCCTTAGT TTGGAAAACT
    GTGACAAACA ACACAACCTC CTACATGCGT CACGTCTGTA TTCTCAACAC AGCGACATCT
    CTCCTAATTG CTGACATTTG GTTCATTGTT ACTGCCTCCA TCAATGTGCA GAAAAGAAAT
    ATCTGTATTG CAGCCACATT CTTCATCCAT TTATTCTACC TATGTGGCTT TTTCTGGATG
    CTCAGCCTCG GCCTCATCCT TTTTTACAGA CTAGTCTTCA TCTTACATAA CACAAGCAAG
    ACATCTCAGA AAGCAGTGGT ATTCTGCCTG GGTTATGGGT GCCCCTTTGT GTTTGCAGTC
    ACCACCATTG CTGTCACATT GCCGTGGAAC ACTTACACCA GAAAGGATGC CTGCTGGCTC
    AACTGGAAGG ACAGCAAAGC TGTCTTGGCC TTTGTCATAC CTGCCCTGGC CATCGTGGCC
    ATGAATTTGT TCATCACTGC AGTTGTGATA ATAAAAATAC TAAGGCCGAA CATTGGGGAC
    AGGACAAACA AACAAGAGAG GAAGACCTTC TTTCAAATTT GCAAAAGTCT TGTCATCTTG
    ACACCACTGT TAGGCCTCAC GTGGGGATTT GGTCTTGCCA CCATCATCAG AAATAGCCAC
    CAGACTTTCC ACATCCTCTT TGCTCTGCTC AATGCCTTGC AGGGACTGTT CATTTTGGTA
    TTTGGGACTC TATGGGACAA CAAGATAAAA GAAGCCCTGC TGAAGAGGAA TTCACTCTCC
    AGATGGAGTT CGCAACAAAC AAAGTCAACA TCCCTGATCT TGGTGACACC AATGTTTTCC
    ATGAGCTATC CATTTTCAAG AACATTCAAC AACTTATGTG GCAAAACAGG AAAATACACA
    GTATCTTCTT CAGAGCCATC CAGTGCTTCC ACAGAGAACA TCTCCAAGTC CGATTCTTTG
    CTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140653.1
    CDS753..4781
    Translation

    Target ORF information:

    RefSeq Version XM_015285167.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285167.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCCATCCC CAATTACTGC AGCTCTCCAC CTCCTGTCTC TACTGGCCGT ATGCTTCCAG 
    ACGTCTCCAG CCTCGGGCTT TGACTCATTC ACCCAATATG CCGTTGGCAG GGAGGTAGGA
    GAGGAGAGCT TCCACCCAGA GCTGCAGAGG CAGAAGCGAC ATGTGATCCT TTTTGACCTT
    CCACCCCTGG AGTATGCCAT TGACATTGAA GTAAGCCTCA CGAATTCATC CTTCCTGGCA
    CCAATCAAGG AGTACTTCAG AAACCTCCAT CTTCCAGTTT CAATCAATAT TTCAGATGTA
    GAAATGACAG TTTCAAGTAT CAACATCACA ACAGTCTGTG TGCCCAGTGA GAACAAGAGC
    TGCTGTTCTT GTGAAAGCGG ATATGCCTGG CCAAGCACGG GGTGCAGTGA CCTGATCAGC
    TGCCCTGCTC TCAGCCGAGC TCCCAACCAG CCATGCGGCT ACACCAGGGA AAGGCCTTTC
    AATGGGCCCT ACTGTGAACC TCAGAATGGA GACTCATGTG GTATGGGAGA GCCCACTGTA
    ATGAATATGT CAGTAAGACT TGACACAGCC TTCCACGATG ATCTCAGGAA TCATTCTTCC
    CAACTATACA GAAAATACAA AGCTGACCTC GATAAAGCGT TTAAAGCTAG CTACGGGTGC
    TTACCGGGCT TTGTGTCAGC AACAACAACT GGTTTCAGGC CTGGGAGTGT TGAGGTGAAC
    TACAGAGTGG AGGCAGGATC AGCGAGCTTC CAACAGCTGG AGCATTCCAG CAGATTTGTT
    ACACAGTACC TGGATGAACC ATACAGCATA GTGCCTACCT CCTTTACAGC AGAAATAACG
    AATCGCACAA ACTTCACAGT GAGCCCTGCC CACATTTTTG AAGGGGATAC CATCCAGATG
    GTGTGTGAGA TAAACACCAC GTCTGAGAAC ATGACCTGGT ATCGTGACAG TCAGATCATC
    CTCAACAGCT CACGGCACTC CGTGAGGACA GACAACAACA CTGCGATGTC ACAGGCATTT
    CTTACAATTA CCAACGTTAC GCAGGAGGAT TCTGGTACCT ACAAATGCAC TTTTGCAAAC
    AGATACCGGT CTTTAAATCT GATATACGAA GGCACCAAAG AAATACAAGT CTCTCCACTA
    CGTATCATCT CATATTCAAA CGACTATAAA GTTCACTGCA ACAGCCCTGA GATACAGGCA
    AACAGCCCTG TGCTGTTCTG TTGCATTGAT GGACAATTAC CACTGCTTAC TGGTGACTGG
    AAGGTGAATG GAGCGATCAG TATTACAGGA GTCCCCAGTT TCAGCAGCAA CTGCACTGAA
    TACAAGCTCA ACATGACGGA CTCGCTGTGC TTGGCCGAGC ATTCAGGTAC GGTGACGACA
    TACACGTGTG AGCTGCAGAC AGGGCACGGC GCCAGGAGCA GCCGGGACAT CCGAGTGATG
    TACTTCCGAG CAGCCCAGGT AAGAATATCT TCCAGCGTAA ACACAACCGT TTCCGTGGGA
    TATGGCTTCA GTCTAAAGTG TGAAAGCGAT ACGAGCAATT ACGACAGCAT CAGCTGGGAA
    ATCCATTCTG GAGATAGAAT ACAAGCAGTA GAATGCGCTG CATGCATTAG AACCAACAAA
    TCCATGGCCA CATCCATGCT CGTGGTGAAC ACTGCCACTC AAGACTGGAA TGGCACCTAC
    ATCTGCACAT TGTCCCAGGA CTCCCTGAAC AGTTCTGCCA GCGTGACAAT CAATGTCATT
    CCGTTGCCCC TGAAGCAGCA CATCCTGATA GACCCCGCTG GAGCGCCTAT CATGTGCGGA
    ACGCCCCAAG CCCTCACCTG CTGCATCAGT GCTAACAGCA TGGGAGATTA TGATGTTAAA
    TTTGTGGTAC AAGAAAAAGA ATTTCAAGCT GAGAAAGAAG AGCAAGGAAA TTTCTTTTGC
    TACTCGTACA ATTACACAGA AACAGAATGC AGGGAAAAGA AGCTGGAGGC GTATTGCAAG
    TTTGTGAACA GCAATGAGCA GGAGGTCAGC AGCAACAGTA TAAATCTGAG CCTGATACCT
    GATAAGGACC TCATTTCCTG CTCAGAAAAC AATACCTTTG GAAAAGAAGG AGACATATTG
    AAAAAGTCGT GCTCTGTTTT AAATACTACT TTTATCCGAG GCAGCAACAT TTACAAGTGC
    TTCAACAGCT CATGGAAGAT GGAAAAGAAT GACTGCGTAT CCGAACAGAT AAACAACCTG
    CTGCTCATGG CAGAGTCCTT GGTCAACAGT CCCAGTGCAA AAGTTGGTCT ACCTAACTTC
    CTTCAGAACC TGCGGAATCA GACAGAACTG CTGCAAAATG ACAATTCTTC TGCAAATCTT
    GCTGCAATTG TTACCATCCT CTACAACATC TCTTCTATTC CAGTAGAAGC AAAGCAGTCC
    ACTATCTCTG ATTACTTCAC TACAGTGGAC ATTATCGTTA CTGATTTCAA AAAGGAACTT
    TGGGCTGACC TGAATGAAGG GCAGGTACCC ATAAGTTCTT TGTTACTGAA TGCGGTGGAA
    AGATTTTCTG AGAACCTCCA ACCAGTTAAT AATACCATTC CACATCTGTC CACTGAGACC
    CTTGAGCTGC AAGGCATGGT TGTCACAGAA CATAGCCACG CAGACTATAA TAAGAACTTC
    AGCCGTCTAG GAAACCTCAC AGCTAATGTG CTGATTGATA ACATTGAGAC TCTGCCTCCA
    AACTCAACCA TTGTCAGTGT GGCCTACTCA GCACTTGGTC ATATTTTGCC TGTGAAGGAG
    AACATGTCTG TGAACAGTTT AGTGATATCA ACAGCTCTGA GCTGCGAGAG AAACCAGAAC
    TTCCATATTA ATATGACCTT TCAGAAAGAA AACATATCTC TGAAGATGCC CCTGTGTGTC
    TTTTGGGACT TCAAACTCAA CGAAAGTAGG GGAGGCTGGA ATGATCGTGG TTGCACGCAA
    ACAGAGGAAG GCAGCAATGT CATTTGTTCC TGCAATCACT TGACACCATT CTCCATTCTG
    ATGTCACCGG ATGAACCTGC TCACAATTCT GTTGAAGATT ACATCACCCA CATTGGCCTG
    GCCATTTCAA TCTTGAGTTT AGTGGTCTGC ATTGTAATTG AATCCTTAGT TTGGAAAACT
    GTGACAAACA ACACAACCTC CTACATGCGT CACGTCTGTA TTCTCAACAC AGCGACATCT
    CTCCTAATTG CTGACATTTG GTTCATTGTT ACTGCCTCCA TCAATGTGCA GAAAAGAAAT
    ATCTGTATTG CAGCCACATT CTTCATCCAT TTATTCTACC TATGTGGCTT TTTCTGGATG
    CTCAGCCTCG GCCTCATCCT TTTTTACAGA CTAGTCTTCA TCTTACATAA CACAAGCAAG
    ACATCTCAGA AAGCAGTGGT ATTCTGCCTG GGTTATGGGT GCCCCTTTGT GTTTGCAGTC
    ACCACCATTG CTGTCACATT GCCGTGGAAC ACTTACACCA GAAAGGATGC CTGCTGGCTC
    AACTGGAAGG ACAGCAAAGC TGTCTTGGCC TTTGTCATAC CTGCCCTGGC CATCGTGGCC
    ATGAATTTGT TCATCACTGC AGTTGTGATA ATAAAAATAC TAAGGCCGAA CATTGGGGAC
    AGGACAAACA AACAAGAGAG GAAGACCTTC TTTCAAATTT GCAAAAGTCT TGTCATCTTG
    ACACCACTGT TAGGCCTCAC GTGGGGATTT GGTCTTGCCA CCATCATCAG AAATAGCCAC
    CAGACTTTCC ACATCCTCTT TGCTCTGCTC AATGCCTTGC AGGGACTGTT CATTTTGGTA
    TTTGGGACTC TATGGGACAA CAAGATAAAA GAAGCCCTGC TGAAGAGGAA TTCACTCTCC
    AGATGGAGTT CGCAACAAAC AAAGTCAACA TCCCTGATCT TGGTGACACC AATGTTTTCC
    ATGAGCTATC CATTTTCAAG AACATTCAAC AACTTATGTG GCAAAACAGG AAAATACACA
    GTATCTTCTT CAGAGCCATC CAGTGCTTCC ACAGAGAACA TCTCCAAGTC CGATTCTTTG
    CTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07171
    Clone ID Related Accession (Same CDS sequence) XM_004935931.2 , XM_015285166.1 , XM_004935931.3 , XM_015285167.1 , XM_004935933.2 , XM_004935933.3 , XM_015285166.2 , XM_025149283.1 , XM_015285167.2
    Accession Version XM_004935933.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product adhesion G protein-coupled receptor F5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004935933.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCCATCCC CAATTACTGC AGCTCTCCAC CTCCTGTCTC TACTGGCCGT ATGCTTCCAG 
    ACGTCTCCAG CCTCGGGCTT TGACTCATTC ACCCAATATG CCGTTGGCAG GGAGGTAGGA
    GAGGAGAGCT TCCACCCAGA GCTGCAGAGG CAGAAGCGAC ATGTGATCCT TTTTGACCTT
    CCACCCCTGG AGTATGCCAT TGACATTGAA GTAAGCCTCA CGAATTCATC CTTCCTGGCA
    CCAATCAAGG AGTACTTCAG AAACCTCCAT CTTCCAGTTT CAATCAATAT TTCAGATGTA
    GAAATGACAG TTTCAAGTAT CAACATCACA ACAGTCTGTG TGCCCAGTGA GAACAAGAGC
    TGCTGTTCTT GTGAAAGCGG ATATGCCTGG CCAAGCACGG GGTGCAGTGA CCTGATCAGC
    TGCCCTGCTC TCAGCCGAGC TCCCAACCAG CCATGCGGCT ACACCAGGGA AAGGCCTTTC
    AATGGGCCCT ACTGTGAACC TCAGAATGGA GACTCATGTG GTATGGGAGA GCCCACTGTA
    ATGAATATGT CAGTAAGACT TGACACAGCC TTCCACGATG ATCTCAGGAA TCATTCTTCC
    CAACTATACA GAAAATACAA AGCTGACCTC GATAAAGCGT TTAAAGCTAG CTACGGGTGC
    TTACCGGGCT TTGTGTCAGC AACAACAACT GGTTTCAGGC CTGGGAGTGT TGAGGTGAAC
    TACAGAGTGG AGGCAGGATC AGCGAGCTTC CAACAGCTGG AGCATTCCAG CAGATTTGTT
    ACACAGTACC TGGATGAACC ATACAGCATA GTGCCTACCT CCTTTACAGC AGAAATAACG
    AATCGCACAA ACTTCACAGT GAGCCCTGCC CACATTTTTG AAGGGGATAC CATCCAGATG
    GTGTGTGAGA TAAACACCAC GTCTGAGAAC ATGACCTGGT ATCGTGACAG TCAGATCATC
    CTCAACAGCT CACGGCACTC CGTGAGGACA GACAACAACA CTGCGATGTC ACAGGCATTT
    CTTACAATTA CCAACGTTAC GCAGGAGGAT TCTGGTACCT ACAAATGCAC TTTTGCAAAC
    AGATACCGGT CTTTAAATCT GATATACGAA GGCACCAAAG AAATACAAGT CTCTCCACTA
    CGTATCATCT CATATTCAAA CGACTATAAA GTTCACTGCA ACAGCCCTGA GATACAGGCA
    AACAGCCCTG TGCTGTTCTG TTGCATTGAT GGACAATTAC CACTGCTTAC TGGTGACTGG
    AAGGTGAATG GAGCGATCAG TATTACAGGA GTCCCCAGTT TCAGCAGCAA CTGCACTGAA
    TACAAGCTCA ACATGACGGA CTCGCTGTGC TTGGCCGAGC ATTCAGGTAC GGTGACGACA
    TACACGTGTG AGCTGCAGAC AGGGCACGGC GCCAGGAGCA GCCGGGACAT CCGAGTGATG
    TACTTCCGAG CAGCCCAGGT AAGAATATCT TCCAGCGTAA ACACAACCGT TTCCGTGGGA
    TATGGCTTCA GTCTAAAGTG TGAAAGCGAT ACGAGCAATT ACGACAGCAT CAGCTGGGAA
    ATCCATTCTG GAGATAGAAT ACAAGCAGTA GAATGCGCTG CATGCATTAG AACCAACAAA
    TCCATGGCCA CATCCATGCT CGTGGTGAAC ACTGCCACTC AAGACTGGAA TGGCACCTAC
    ATCTGCACAT TGTCCCAGGA CTCCCTGAAC AGTTCTGCCA GCGTGACAAT CAATGTCATT
    CCGTTGCCCC TGAAGCAGCA CATCCTGATA GACCCCGCTG GAGCGCCTAT CATGTGCGGA
    ACGCCCCAAG CCCTCACCTG CTGCATCAGT GCTAACAGCA TGGGAGATTA TGATGTTAAA
    TTTGTGGTAC AAGAAAAAGA ATTTCAAGCT GAGAAAGAAG AGCAAGGAAA TTTCTTTTGC
    TACTCGTACA ATTACACAGA AACAGAATGC AGGGAAAAGA AGCTGGAGGC GTATTGCAAG
    TTTGTGAACA GCAATGAGCA GGAGGTCAGC AGCAACAGTA TAAATCTGAG CCTGATACCT
    GATAAGGACC TCATTTCCTG CTCAGAAAAC AATACCTTTG GAAAAGAAGG AGACATATTG
    AAAAAGTCGT GCTCTGTTTT AAATACTACT TTTATCCGAG GCAGCAACAT TTACAAGTGC
    TTCAACAGCT CATGGAAGAT GGAAAAGAAT GACTGCGTAT CCGAACAGAT AAACAACCTG
    CTGCTCATGG CAGAGTCCTT GGTCAACAGT CCCAGTGCAA AAGTTGGTCT ACCTAACTTC
    CTTCAGAACC TGCGGAATCA GACAGAACTG CTGCAAAATG ACAATTCTTC TGCAAATCTT
    GCTGCAATTG TTACCATCCT CTACAACATC TCTTCTATTC CAGTAGAAGC AAAGCAGTCC
    ACTATCTCTG ATTACTTCAC TACAGTGGAC ATTATCGTTA CTGATTTCAA AAAGGAACTT
    TGGGCTGACC TGAATGAAGG GCAGGTACCC ATAAGTTCTT TGTTACTGAA TGCGGTGGAA
    AGATTTTCTG AGAACCTCCA ACCAGTTAAT AATACCATTC CACATCTGTC CACTGAGACC
    CTTGAGCTGC AAGGCATGGT TGTCACAGAA CATAGCCACG CAGACTATAA TAAGAACTTC
    AGCCGTCTAG GAAACCTCAC AGCTAATGTG CTGATTGATA ACATTGAGAC TCTGCCTCCA
    AACTCAACCA TTGTCAGTGT GGCCTACTCA GCACTTGGTC ATATTTTGCC TGTGAAGGAG
    AACATGTCTG TGAACAGTTT AGTGATATCA ACAGCTCTGA GCTGCGAGAG AAACCAGAAC
    TTCCATATTA ATATGACCTT TCAGAAAGAA AACATATCTC TGAAGATGCC CCTGTGTGTC
    TTTTGGGACT TCAAACTCAA CGAAAGTAGG GGAGGCTGGA ATGATCGTGG TTGCACGCAA
    ACAGAGGAAG GCAGCAATGT CATTTGTTCC TGCAATCACT TGACACCATT CTCCATTCTG
    ATGTCACCGG ATGAACCTGC TCACAATTCT GTTGAAGATT ACATCACCCA CATTGGCCTG
    GCCATTTCAA TCTTGAGTTT AGTGGTCTGC ATTGTAATTG AATCCTTAGT TTGGAAAACT
    GTGACAAACA ACACAACCTC CTACATGCGT CACGTCTGTA TTCTCAACAC AGCGACATCT
    CTCCTAATTG CTGACATTTG GTTCATTGTT ACTGCCTCCA TCAATGTGCA GAAAAGAAAT
    ATCTGTATTG CAGCCACATT CTTCATCCAT TTATTCTACC TATGTGGCTT TTTCTGGATG
    CTCAGCCTCG GCCTCATCCT TTTTTACAGA CTAGTCTTCA TCTTACATAA CACAAGCAAG
    ACATCTCAGA AAGCAGTGGT ATTCTGCCTG GGTTATGGGT GCCCCTTTGT GTTTGCAGTC
    ACCACCATTG CTGTCACATT GCCGTGGAAC ACTTACACCA GAAAGGATGC CTGCTGGCTC
    AACTGGAAGG ACAGCAAAGC TGTCTTGGCC TTTGTCATAC CTGCCCTGGC CATCGTGGCC
    ATGAATTTGT TCATCACTGC AGTTGTGATA ATAAAAATAC TAAGGCCGAA CATTGGGGAC
    AGGACAAACA AACAAGAGAG GAAGACCTTC TTTCAAATTT GCAAAAGTCT TGTCATCTTG
    ACACCACTGT TAGGCCTCAC GTGGGGATTT GGTCTTGCCA CCATCATCAG AAATAGCCAC
    CAGACTTTCC ACATCCTCTT TGCTCTGCTC AATGCCTTGC AGGGACTGTT CATTTTGGTA
    TTTGGGACTC TATGGGACAA CAAGATAAAA GAAGCCCTGC TGAAGAGGAA TTCACTCTCC
    AGATGGAGTT CGCAACAAAC AAAGTCAACA TCCCTGATCT TGGTGACACC AATGTTTTCC
    ATGAGCTATC CATTTTCAAG AACATTCAAC AACTTATGTG GCAAAACAGG AAAATACACA
    GTATCTTCTT CAGAGCCATC CAGTGCTTCC ACAGAGAACA TCTCCAAGTC CGATTCTTTG
    CTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004935990.1
    CDS538..4566
    Translation

    Target ORF information:

    RefSeq Version XM_004935933.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004935933.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCCATCCC CAATTACTGC AGCTCTCCAC CTCCTGTCTC TACTGGCCGT ATGCTTCCAG 
    ACGTCTCCAG CCTCGGGCTT TGACTCATTC ACCCAATATG CCGTTGGCAG GGAGGTAGGA
    GAGGAGAGCT TCCACCCAGA GCTGCAGAGG CAGAAGCGAC ATGTGATCCT TTTTGACCTT
    CCACCCCTGG AGTATGCCAT TGACATTGAA GTAAGCCTCA CGAATTCATC CTTCCTGGCA
    CCAATCAAGG AGTACTTCAG AAACCTCCAT CTTCCAGTTT CAATCAATAT TTCAGATGTA
    GAAATGACAG TTTCAAGTAT CAACATCACA ACAGTCTGTG TGCCCAGTGA GAACAAGAGC
    TGCTGTTCTT GTGAAAGCGG ATATGCCTGG CCAAGCACGG GGTGCAGTGA CCTGATCAGC
    TGCCCTGCTC TCAGCCGAGC TCCCAACCAG CCATGCGGCT ACACCAGGGA AAGGCCTTTC
    AATGGGCCCT ACTGTGAACC TCAGAATGGA GACTCATGTG GTATGGGAGA GCCCACTGTA
    ATGAATATGT CAGTAAGACT TGACACAGCC TTCCACGATG ATCTCAGGAA TCATTCTTCC
    CAACTATACA GAAAATACAA AGCTGACCTC GATAAAGCGT TTAAAGCTAG CTACGGGTGC
    TTACCGGGCT TTGTGTCAGC AACAACAACT GGTTTCAGGC CTGGGAGTGT TGAGGTGAAC
    TACAGAGTGG AGGCAGGATC AGCGAGCTTC CAACAGCTGG AGCATTCCAG CAGATTTGTT
    ACACAGTACC TGGATGAACC ATACAGCATA GTGCCTACCT CCTTTACAGC AGAAATAACG
    AATCGCACAA ACTTCACAGT GAGCCCTGCC CACATTTTTG AAGGGGATAC CATCCAGATG
    GTGTGTGAGA TAAACACCAC GTCTGAGAAC ATGACCTGGT ATCGTGACAG TCAGATCATC
    CTCAACAGCT CACGGCACTC CGTGAGGACA GACAACAACA CTGCGATGTC ACAGGCATTT
    CTTACAATTA CCAACGTTAC GCAGGAGGAT TCTGGTACCT ACAAATGCAC TTTTGCAAAC
    AGATACCGGT CTTTAAATCT GATATACGAA GGCACCAAAG AAATACAAGT CTCTCCACTA
    CGTATCATCT CATATTCAAA CGACTATAAA GTTCACTGCA ACAGCCCTGA GATACAGGCA
    AACAGCCCTG TGCTGTTCTG TTGCATTGAT GGACAATTAC CACTGCTTAC TGGTGACTGG
    AAGGTGAATG GAGCGATCAG TATTACAGGA GTCCCCAGTT TCAGCAGCAA CTGCACTGAA
    TACAAGCTCA ACATGACGGA CTCGCTGTGC TTGGCCGAGC ATTCAGGTAC GGTGACGACA
    TACACGTGTG AGCTGCAGAC AGGGCACGGC GCCAGGAGCA GCCGGGACAT CCGAGTGATG
    TACTTCCGAG CAGCCCAGGT AAGAATATCT TCCAGCGTAA ACACAACCGT TTCCGTGGGA
    TATGGCTTCA GTCTAAAGTG TGAAAGCGAT ACGAGCAATT ACGACAGCAT CAGCTGGGAA
    ATCCATTCTG GAGATAGAAT ACAAGCAGTA GAATGCGCTG CATGCATTAG AACCAACAAA
    TCCATGGCCA CATCCATGCT CGTGGTGAAC ACTGCCACTC AAGACTGGAA TGGCACCTAC
    ATCTGCACAT TGTCCCAGGA CTCCCTGAAC AGTTCTGCCA GCGTGACAAT CAATGTCATT
    CCGTTGCCCC TGAAGCAGCA CATCCTGATA GACCCCGCTG GAGCGCCTAT CATGTGCGGA
    ACGCCCCAAG CCCTCACCTG CTGCATCAGT GCTAACAGCA TGGGAGATTA TGATGTTAAA
    TTTGTGGTAC AAGAAAAAGA ATTTCAAGCT GAGAAAGAAG AGCAAGGAAA TTTCTTTTGC
    TACTCGTACA ATTACACAGA AACAGAATGC AGGGAAAAGA AGCTGGAGGC GTATTGCAAG
    TTTGTGAACA GCAATGAGCA GGAGGTCAGC AGCAACAGTA TAAATCTGAG CCTGATACCT
    GATAAGGACC TCATTTCCTG CTCAGAAAAC AATACCTTTG GAAAAGAAGG AGACATATTG
    AAAAAGTCGT GCTCTGTTTT AAATACTACT TTTATCCGAG GCAGCAACAT TTACAAGTGC
    TTCAACAGCT CATGGAAGAT GGAAAAGAAT GACTGCGTAT CCGAACAGAT AAACAACCTG
    CTGCTCATGG CAGAGTCCTT GGTCAACAGT CCCAGTGCAA AAGTTGGTCT ACCTAACTTC
    CTTCAGAACC TGCGGAATCA GACAGAACTG CTGCAAAATG ACAATTCTTC TGCAAATCTT
    GCTGCAATTG TTACCATCCT CTACAACATC TCTTCTATTC CAGTAGAAGC AAAGCAGTCC
    ACTATCTCTG ATTACTTCAC TACAGTGGAC ATTATCGTTA CTGATTTCAA AAAGGAACTT
    TGGGCTGACC TGAATGAAGG GCAGGTACCC ATAAGTTCTT TGTTACTGAA TGCGGTGGAA
    AGATTTTCTG AGAACCTCCA ACCAGTTAAT AATACCATTC CACATCTGTC CACTGAGACC
    CTTGAGCTGC AAGGCATGGT TGTCACAGAA CATAGCCACG CAGACTATAA TAAGAACTTC
    AGCCGTCTAG GAAACCTCAC AGCTAATGTG CTGATTGATA ACATTGAGAC TCTGCCTCCA
    AACTCAACCA TTGTCAGTGT GGCCTACTCA GCACTTGGTC ATATTTTGCC TGTGAAGGAG
    AACATGTCTG TGAACAGTTT AGTGATATCA ACAGCTCTGA GCTGCGAGAG AAACCAGAAC
    TTCCATATTA ATATGACCTT TCAGAAAGAA AACATATCTC TGAAGATGCC CCTGTGTGTC
    TTTTGGGACT TCAAACTCAA CGAAAGTAGG GGAGGCTGGA ATGATCGTGG TTGCACGCAA
    ACAGAGGAAG GCAGCAATGT CATTTGTTCC TGCAATCACT TGACACCATT CTCCATTCTG
    ATGTCACCGG ATGAACCTGC TCACAATTCT GTTGAAGATT ACATCACCCA CATTGGCCTG
    GCCATTTCAA TCTTGAGTTT AGTGGTCTGC ATTGTAATTG AATCCTTAGT TTGGAAAACT
    GTGACAAACA ACACAACCTC CTACATGCGT CACGTCTGTA TTCTCAACAC AGCGACATCT
    CTCCTAATTG CTGACATTTG GTTCATTGTT ACTGCCTCCA TCAATGTGCA GAAAAGAAAT
    ATCTGTATTG CAGCCACATT CTTCATCCAT TTATTCTACC TATGTGGCTT TTTCTGGATG
    CTCAGCCTCG GCCTCATCCT TTTTTACAGA CTAGTCTTCA TCTTACATAA CACAAGCAAG
    ACATCTCAGA AAGCAGTGGT ATTCTGCCTG GGTTATGGGT GCCCCTTTGT GTTTGCAGTC
    ACCACCATTG CTGTCACATT GCCGTGGAAC ACTTACACCA GAAAGGATGC CTGCTGGCTC
    AACTGGAAGG ACAGCAAAGC TGTCTTGGCC TTTGTCATAC CTGCCCTGGC CATCGTGGCC
    ATGAATTTGT TCATCACTGC AGTTGTGATA ATAAAAATAC TAAGGCCGAA CATTGGGGAC
    AGGACAAACA AACAAGAGAG GAAGACCTTC TTTCAAATTT GCAAAAGTCT TGTCATCTTG
    ACACCACTGT TAGGCCTCAC GTGGGGATTT GGTCTTGCCA CCATCATCAG AAATAGCCAC
    CAGACTTTCC ACATCCTCTT TGCTCTGCTC AATGCCTTGC AGGGACTGTT CATTTTGGTA
    TTTGGGACTC TATGGGACAA CAAGATAAAA GAAGCCCTGC TGAAGAGGAA TTCACTCTCC
    AGATGGAGTT CGCAACAAAC AAAGTCAACA TCCCTGATCT TGGTGACACC AATGTTTTCC
    ATGAGCTATC CATTTTCAAG AACATTCAAC AACTTATGTG GCAAAACAGG AAAATACACA
    GTATCTTCTT CAGAGCCATC CAGTGCTTCC ACAGAGAACA TCTCCAAGTC CGATTCTTTG
    CTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07171
    Clone ID Related Accession (Same CDS sequence) XM_004935931.2 , XM_015285166.1 , XM_004935931.3 , XM_015285167.1 , XM_004935933.2 , XM_004935933.3 , XM_015285166.2 , XM_025149283.1 , XM_015285167.2
    Accession Version XM_004935933.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product adhesion G protein-coupled receptor F5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004935933.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCCATCCC CAATTACTGC AGCTCTCCAC CTCCTGTCTC TACTGGCCGT ATGCTTCCAG 
    ACGTCTCCAG CCTCGGGCTT TGACTCATTC ACCCAATATG CCGTTGGCAG GGAGGTAGGA
    GAGGAGAGCT TCCACCCAGA GCTGCAGAGG CAGAAGCGAC ATGTGATCCT TTTTGACCTT
    CCACCCCTGG AGTATGCCAT TGACATTGAA GTAAGCCTCA CGAATTCATC CTTCCTGGCA
    CCAATCAAGG AGTACTTCAG AAACCTCCAT CTTCCAGTTT CAATCAATAT TTCAGATGTA
    GAAATGACAG TTTCAAGTAT CAACATCACA ACAGTCTGTG TGCCCAGTGA GAACAAGAGC
    TGCTGTTCTT GTGAAAGCGG ATATGCCTGG CCAAGCACGG GGTGCAGTGA CCTGATCAGC
    TGCCCTGCTC TCAGCCGAGC TCCCAACCAG CCATGCGGCT ACACCAGGGA AAGGCCTTTC
    AATGGGCCCT ACTGTGAACC TCAGAATGGA GACTCATGTG GTATGGGAGA GCCCACTGTA
    ATGAATATGT CAGTAAGACT TGACACAGCC TTCCACGATG ATCTCAGGAA TCATTCTTCC
    CAACTATACA GAAAATACAA AGCTGACCTC GATAAAGCGT TTAAAGCTAG CTACGGGTGC
    TTACCGGGCT TTGTGTCAGC AACAACAACT GGTTTCAGGC CTGGGAGTGT TGAGGTGAAC
    TACAGAGTGG AGGCAGGATC AGCGAGCTTC CAACAGCTGG AGCATTCCAG CAGATTTGTT
    ACACAGTACC TGGATGAACC ATACAGCATA GTGCCTACCT CCTTTACAGC AGAAATAACG
    AATCGCACAA ACTTCACAGT GAGCCCTGCC CACATTTTTG AAGGGGATAC CATCCAGATG
    GTGTGTGAGA TAAACACCAC GTCTGAGAAC ATGACCTGGT ATCGTGACAG TCAGATCATC
    CTCAACAGCT CACGGCACTC CGTGAGGACA GACAACAACA CTGCGATGTC ACAGGCATTT
    CTTACAATTA CCAACGTTAC GCAGGAGGAT TCTGGTACCT ACAAATGCAC TTTTGCAAAC
    AGATACCGGT CTTTAAATCT GATATACGAA GGCACCAAAG AAATACAAGT CTCTCCACTA
    CGTATCATCT CATATTCAAA CGACTATAAA GTTCACTGCA ACAGCCCTGA GATACAGGCA
    AACAGCCCTG TGCTGTTCTG TTGCATTGAT GGACAATTAC CACTGCTTAC TGGTGACTGG
    AAGGTGAATG GAGCGATCAG TATTACAGGA GTCCCCAGTT TCAGCAGCAA CTGCACTGAA
    TACAAGCTCA ACATGACGGA CTCGCTGTGC TTGGCCGAGC ATTCAGGTAC GGTGACGACA
    TACACGTGTG AGCTGCAGAC AGGGCACGGC GCCAGGAGCA GCCGGGACAT CCGAGTGATG
    TACTTCCGAG CAGCCCAGGT AAGAATATCT TCCAGCGTAA ACACAACCGT TTCCGTGGGA
    TATGGCTTCA GTCTAAAGTG TGAAAGCGAT ACGAGCAATT ACGACAGCAT CAGCTGGGAA
    ATCCATTCTG GAGATAGAAT ACAAGCAGTA GAATGCGCTG CATGCATTAG AACCAACAAA
    TCCATGGCCA CATCCATGCT CGTGGTGAAC ACTGCCACTC AAGACTGGAA TGGCACCTAC
    ATCTGCACAT TGTCCCAGGA CTCCCTGAAC AGTTCTGCCA GCGTGACAAT CAATGTCATT
    CCGTTGCCCC TGAAGCAGCA CATCCTGATA GACCCCGCTG GAGCGCCTAT CATGTGCGGA
    ACGCCCCAAG CCCTCACCTG CTGCATCAGT GCTAACAGCA TGGGAGATTA TGATGTTAAA
    TTTGTGGTAC AAGAAAAAGA ATTTCAAGCT GAGAAAGAAG AGCAAGGAAA TTTCTTTTGC
    TACTCGTACA ATTACACAGA AACAGAATGC AGGGAAAAGA AGCTGGAGGC GTATTGCAAG
    TTTGTGAACA GCAATGAGCA GGAGGTCAGC AGCAACAGTA TAAATCTGAG CCTGATACCT
    GATAAGGACC TCATTTCCTG CTCAGAAAAC AATACCTTTG GAAAAGAAGG AGACATATTG
    AAAAAGTCGT GCTCTGTTTT AAATACTACT TTTATCCGAG GCAGCAACAT TTACAAGTGC
    TTCAACAGCT CATGGAAGAT GGAAAAGAAT GACTGCGTAT CCGAACAGAT AAACAACCTG
    CTGCTCATGG CAGAGTCCTT GGTCAACAGT CCCAGTGCAA AAGTTGGTCT ACCTAACTTC
    CTTCAGAACC TGCGGAATCA GACAGAACTG CTGCAAAATG ACAATTCTTC TGCAAATCTT
    GCTGCAATTG TTACCATCCT CTACAACATC TCTTCTATTC CAGTAGAAGC AAAGCAGTCC
    ACTATCTCTG ATTACTTCAC TACAGTGGAC ATTATCGTTA CTGATTTCAA AAAGGAACTT
    TGGGCTGACC TGAATGAAGG GCAGGTACCC ATAAGTTCTT TGTTACTGAA TGCGGTGGAA
    AGATTTTCTG AGAACCTCCA ACCAGTTAAT AATACCATTC CACATCTGTC CACTGAGACC
    CTTGAGCTGC AAGGCATGGT TGTCACAGAA CATAGCCACG CAGACTATAA TAAGAACTTC
    AGCCGTCTAG GAAACCTCAC AGCTAATGTG CTGATTGATA ACATTGAGAC TCTGCCTCCA
    AACTCAACCA TTGTCAGTGT GGCCTACTCA GCACTTGGTC ATATTTTGCC TGTGAAGGAG
    AACATGTCTG TGAACAGTTT AGTGATATCA ACAGCTCTGA GCTGCGAGAG AAACCAGAAC
    TTCCATATTA ATATGACCTT TCAGAAAGAA AACATATCTC TGAAGATGCC CCTGTGTGTC
    TTTTGGGACT TCAAACTCAA CGAAAGTAGG GGAGGCTGGA ATGATCGTGG TTGCACGCAA
    ACAGAGGAAG GCAGCAATGT CATTTGTTCC TGCAATCACT TGACACCATT CTCCATTCTG
    ATGTCACCGG ATGAACCTGC TCACAATTCT GTTGAAGATT ACATCACCCA CATTGGCCTG
    GCCATTTCAA TCTTGAGTTT AGTGGTCTGC ATTGTAATTG AATCCTTAGT TTGGAAAACT
    GTGACAAACA ACACAACCTC CTACATGCGT CACGTCTGTA TTCTCAACAC AGCGACATCT
    CTCCTAATTG CTGACATTTG GTTCATTGTT ACTGCCTCCA TCAATGTGCA GAAAAGAAAT
    ATCTGTATTG CAGCCACATT CTTCATCCAT TTATTCTACC TATGTGGCTT TTTCTGGATG
    CTCAGCCTCG GCCTCATCCT TTTTTACAGA CTAGTCTTCA TCTTACATAA CACAAGCAAG
    ACATCTCAGA AAGCAGTGGT ATTCTGCCTG GGTTATGGGT GCCCCTTTGT GTTTGCAGTC
    ACCACCATTG CTGTCACATT GCCGTGGAAC ACTTACACCA GAAAGGATGC CTGCTGGCTC
    AACTGGAAGG ACAGCAAAGC TGTCTTGGCC TTTGTCATAC CTGCCCTGGC CATCGTGGCC
    ATGAATTTGT TCATCACTGC AGTTGTGATA ATAAAAATAC TAAGGCCGAA CATTGGGGAC
    AGGACAAACA AACAAGAGAG GAAGACCTTC TTTCAAATTT GCAAAAGTCT TGTCATCTTG
    ACACCACTGT TAGGCCTCAC GTGGGGATTT GGTCTTGCCA CCATCATCAG AAATAGCCAC
    CAGACTTTCC ACATCCTCTT TGCTCTGCTC AATGCCTTGC AGGGACTGTT CATTTTGGTA
    TTTGGGACTC TATGGGACAA CAAGATAAAA GAAGCCCTGC TGAAGAGGAA TTCACTCTCC
    AGATGGAGTT CGCAACAAAC AAAGTCAACA TCCCTGATCT TGGTGACACC AATGTTTTCC
    ATGAGCTATC CATTTTCAAG AACATTCAAC AACTTATGTG GCAAAACAGG AAAATACACA
    GTATCTTCTT CAGAGCCATC CAGTGCTTCC ACAGAGAACA TCTCCAAGTC CGATTCTTTG
    CTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004935990.1
    CDS529..4557
    Translation

    Target ORF information:

    RefSeq Version XM_004935933.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004935933.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCCATCCC CAATTACTGC AGCTCTCCAC CTCCTGTCTC TACTGGCCGT ATGCTTCCAG 
    ACGTCTCCAG CCTCGGGCTT TGACTCATTC ACCCAATATG CCGTTGGCAG GGAGGTAGGA
    GAGGAGAGCT TCCACCCAGA GCTGCAGAGG CAGAAGCGAC ATGTGATCCT TTTTGACCTT
    CCACCCCTGG AGTATGCCAT TGACATTGAA GTAAGCCTCA CGAATTCATC CTTCCTGGCA
    CCAATCAAGG AGTACTTCAG AAACCTCCAT CTTCCAGTTT CAATCAATAT TTCAGATGTA
    GAAATGACAG TTTCAAGTAT CAACATCACA ACAGTCTGTG TGCCCAGTGA GAACAAGAGC
    TGCTGTTCTT GTGAAAGCGG ATATGCCTGG CCAAGCACGG GGTGCAGTGA CCTGATCAGC
    TGCCCTGCTC TCAGCCGAGC TCCCAACCAG CCATGCGGCT ACACCAGGGA AAGGCCTTTC
    AATGGGCCCT ACTGTGAACC TCAGAATGGA GACTCATGTG GTATGGGAGA GCCCACTGTA
    ATGAATATGT CAGTAAGACT TGACACAGCC TTCCACGATG ATCTCAGGAA TCATTCTTCC
    CAACTATACA GAAAATACAA AGCTGACCTC GATAAAGCGT TTAAAGCTAG CTACGGGTGC
    TTACCGGGCT TTGTGTCAGC AACAACAACT GGTTTCAGGC CTGGGAGTGT TGAGGTGAAC
    TACAGAGTGG AGGCAGGATC AGCGAGCTTC CAACAGCTGG AGCATTCCAG CAGATTTGTT
    ACACAGTACC TGGATGAACC ATACAGCATA GTGCCTACCT CCTTTACAGC AGAAATAACG
    AATCGCACAA ACTTCACAGT GAGCCCTGCC CACATTTTTG AAGGGGATAC CATCCAGATG
    GTGTGTGAGA TAAACACCAC GTCTGAGAAC ATGACCTGGT ATCGTGACAG TCAGATCATC
    CTCAACAGCT CACGGCACTC CGTGAGGACA GACAACAACA CTGCGATGTC ACAGGCATTT
    CTTACAATTA CCAACGTTAC GCAGGAGGAT TCTGGTACCT ACAAATGCAC TTTTGCAAAC
    AGATACCGGT CTTTAAATCT GATATACGAA GGCACCAAAG AAATACAAGT CTCTCCACTA
    CGTATCATCT CATATTCAAA CGACTATAAA GTTCACTGCA ACAGCCCTGA GATACAGGCA
    AACAGCCCTG TGCTGTTCTG TTGCATTGAT GGACAATTAC CACTGCTTAC TGGTGACTGG
    AAGGTGAATG GAGCGATCAG TATTACAGGA GTCCCCAGTT TCAGCAGCAA CTGCACTGAA
    TACAAGCTCA ACATGACGGA CTCGCTGTGC TTGGCCGAGC ATTCAGGTAC GGTGACGACA
    TACACGTGTG AGCTGCAGAC AGGGCACGGC GCCAGGAGCA GCCGGGACAT CCGAGTGATG
    TACTTCCGAG CAGCCCAGGT AAGAATATCT TCCAGCGTAA ACACAACCGT TTCCGTGGGA
    TATGGCTTCA GTCTAAAGTG TGAAAGCGAT ACGAGCAATT ACGACAGCAT CAGCTGGGAA
    ATCCATTCTG GAGATAGAAT ACAAGCAGTA GAATGCGCTG CATGCATTAG AACCAACAAA
    TCCATGGCCA CATCCATGCT CGTGGTGAAC ACTGCCACTC AAGACTGGAA TGGCACCTAC
    ATCTGCACAT TGTCCCAGGA CTCCCTGAAC AGTTCTGCCA GCGTGACAAT CAATGTCATT
    CCGTTGCCCC TGAAGCAGCA CATCCTGATA GACCCCGCTG GAGCGCCTAT CATGTGCGGA
    ACGCCCCAAG CCCTCACCTG CTGCATCAGT GCTAACAGCA TGGGAGATTA TGATGTTAAA
    TTTGTGGTAC AAGAAAAAGA ATTTCAAGCT GAGAAAGAAG AGCAAGGAAA TTTCTTTTGC
    TACTCGTACA ATTACACAGA AACAGAATGC AGGGAAAAGA AGCTGGAGGC GTATTGCAAG
    TTTGTGAACA GCAATGAGCA GGAGGTCAGC AGCAACAGTA TAAATCTGAG CCTGATACCT
    GATAAGGACC TCATTTCCTG CTCAGAAAAC AATACCTTTG GAAAAGAAGG AGACATATTG
    AAAAAGTCGT GCTCTGTTTT AAATACTACT TTTATCCGAG GCAGCAACAT TTACAAGTGC
    TTCAACAGCT CATGGAAGAT GGAAAAGAAT GACTGCGTAT CCGAACAGAT AAACAACCTG
    CTGCTCATGG CAGAGTCCTT GGTCAACAGT CCCAGTGCAA AAGTTGGTCT ACCTAACTTC
    CTTCAGAACC TGCGGAATCA GACAGAACTG CTGCAAAATG ACAATTCTTC TGCAAATCTT
    GCTGCAATTG TTACCATCCT CTACAACATC TCTTCTATTC CAGTAGAAGC AAAGCAGTCC
    ACTATCTCTG ATTACTTCAC TACAGTGGAC ATTATCGTTA CTGATTTCAA AAAGGAACTT
    TGGGCTGACC TGAATGAAGG GCAGGTACCC ATAAGTTCTT TGTTACTGAA TGCGGTGGAA
    AGATTTTCTG AGAACCTCCA ACCAGTTAAT AATACCATTC CACATCTGTC CACTGAGACC
    CTTGAGCTGC AAGGCATGGT TGTCACAGAA CATAGCCACG CAGACTATAA TAAGAACTTC
    AGCCGTCTAG GAAACCTCAC AGCTAATGTG CTGATTGATA ACATTGAGAC TCTGCCTCCA
    AACTCAACCA TTGTCAGTGT GGCCTACTCA GCACTTGGTC ATATTTTGCC TGTGAAGGAG
    AACATGTCTG TGAACAGTTT AGTGATATCA ACAGCTCTGA GCTGCGAGAG AAACCAGAAC
    TTCCATATTA ATATGACCTT TCAGAAAGAA AACATATCTC TGAAGATGCC CCTGTGTGTC
    TTTTGGGACT TCAAACTCAA CGAAAGTAGG GGAGGCTGGA ATGATCGTGG TTGCACGCAA
    ACAGAGGAAG GCAGCAATGT CATTTGTTCC TGCAATCACT TGACACCATT CTCCATTCTG
    ATGTCACCGG ATGAACCTGC TCACAATTCT GTTGAAGATT ACATCACCCA CATTGGCCTG
    GCCATTTCAA TCTTGAGTTT AGTGGTCTGC ATTGTAATTG AATCCTTAGT TTGGAAAACT
    GTGACAAACA ACACAACCTC CTACATGCGT CACGTCTGTA TTCTCAACAC AGCGACATCT
    CTCCTAATTG CTGACATTTG GTTCATTGTT ACTGCCTCCA TCAATGTGCA GAAAAGAAAT
    ATCTGTATTG CAGCCACATT CTTCATCCAT TTATTCTACC TATGTGGCTT TTTCTGGATG
    CTCAGCCTCG GCCTCATCCT TTTTTACAGA CTAGTCTTCA TCTTACATAA CACAAGCAAG
    ACATCTCAGA AAGCAGTGGT ATTCTGCCTG GGTTATGGGT GCCCCTTTGT GTTTGCAGTC
    ACCACCATTG CTGTCACATT GCCGTGGAAC ACTTACACCA GAAAGGATGC CTGCTGGCTC
    AACTGGAAGG ACAGCAAAGC TGTCTTGGCC TTTGTCATAC CTGCCCTGGC CATCGTGGCC
    ATGAATTTGT TCATCACTGC AGTTGTGATA ATAAAAATAC TAAGGCCGAA CATTGGGGAC
    AGGACAAACA AACAAGAGAG GAAGACCTTC TTTCAAATTT GCAAAAGTCT TGTCATCTTG
    ACACCACTGT TAGGCCTCAC GTGGGGATTT GGTCTTGCCA CCATCATCAG AAATAGCCAC
    CAGACTTTCC ACATCCTCTT TGCTCTGCTC AATGCCTTGC AGGGACTGTT CATTTTGGTA
    TTTGGGACTC TATGGGACAA CAAGATAAAA GAAGCCCTGC TGAAGAGGAA TTCACTCTCC
    AGATGGAGTT CGCAACAAAC AAAGTCAACA TCCCTGATCT TGGTGACACC AATGTTTTCC
    ATGAGCTATC CATTTTCAAG AACATTCAAC AACTTATGTG GCAAAACAGG AAAATACACA
    GTATCTTCTT CAGAGCCATC CAGTGCTTCC ACAGAGAACA TCTCCAAGTC CGATTCTTTG
    CTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07171
    Clone ID Related Accession (Same CDS sequence) XM_004935931.2 , XM_015285166.1 , XM_004935931.3 , XM_015285167.1 , XM_004935933.2 , XM_004935933.3 , XM_015285166.2 , XM_025149283.1 , XM_015285167.2
    Accession Version XM_015285166.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product adhesion G protein-coupled receptor F5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285166.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCCATCCC CAATTACTGC AGCTCTCCAC CTCCTGTCTC TACTGGCCGT ATGCTTCCAG 
    ACGTCTCCAG CCTCGGGCTT TGACTCATTC ACCCAATATG CCGTTGGCAG GGAGGTAGGA
    GAGGAGAGCT TCCACCCAGA GCTGCAGAGG CAGAAGCGAC ATGTGATCCT TTTTGACCTT
    CCACCCCTGG AGTATGCCAT TGACATTGAA GTAAGCCTCA CGAATTCATC CTTCCTGGCA
    CCAATCAAGG AGTACTTCAG AAACCTCCAT CTTCCAGTTT CAATCAATAT TTCAGATGTA
    GAAATGACAG TTTCAAGTAT CAACATCACA ACAGTCTGTG TGCCCAGTGA GAACAAGAGC
    TGCTGTTCTT GTGAAAGCGG ATATGCCTGG CCAAGCACGG GGTGCAGTGA CCTGATCAGC
    TGCCCTGCTC TCAGCCGAGC TCCCAACCAG CCATGCGGCT ACACCAGGGA AAGGCCTTTC
    AATGGGCCCT ACTGTGAACC TCAGAATGGA GACTCATGTG GTATGGGAGA GCCCACTGTA
    ATGAATATGT CAGTAAGACT TGACACAGCC TTCCACGATG ATCTCAGGAA TCATTCTTCC
    CAACTATACA GAAAATACAA AGCTGACCTC GATAAAGCGT TTAAAGCTAG CTACGGGTGC
    TTACCGGGCT TTGTGTCAGC AACAACAACT GGTTTCAGGC CTGGGAGTGT TGAGGTGAAC
    TACAGAGTGG AGGCAGGATC AGCGAGCTTC CAACAGCTGG AGCATTCCAG CAGATTTGTT
    ACACAGTACC TGGATGAACC ATACAGCATA GTGCCTACCT CCTTTACAGC AGAAATAACG
    AATCGCACAA ACTTCACAGT GAGCCCTGCC CACATTTTTG AAGGGGATAC CATCCAGATG
    GTGTGTGAGA TAAACACCAC GTCTGAGAAC ATGACCTGGT ATCGTGACAG TCAGATCATC
    CTCAACAGCT CACGGCACTC CGTGAGGACA GACAACAACA CTGCGATGTC ACAGGCATTT
    CTTACAATTA CCAACGTTAC GCAGGAGGAT TCTGGTACCT ACAAATGCAC TTTTGCAAAC
    AGATACCGGT CTTTAAATCT GATATACGAA GGCACCAAAG AAATACAAGT CTCTCCACTA
    CGTATCATCT CATATTCAAA CGACTATAAA GTTCACTGCA ACAGCCCTGA GATACAGGCA
    AACAGCCCTG TGCTGTTCTG TTGCATTGAT GGACAATTAC CACTGCTTAC TGGTGACTGG
    AAGGTGAATG GAGCGATCAG TATTACAGGA GTCCCCAGTT TCAGCAGCAA CTGCACTGAA
    TACAAGCTCA ACATGACGGA CTCGCTGTGC TTGGCCGAGC ATTCAGGTAC GGTGACGACA
    TACACGTGTG AGCTGCAGAC AGGGCACGGC GCCAGGAGCA GCCGGGACAT CCGAGTGATG
    TACTTCCGAG CAGCCCAGGT AAGAATATCT TCCAGCGTAA ACACAACCGT TTCCGTGGGA
    TATGGCTTCA GTCTAAAGTG TGAAAGCGAT ACGAGCAATT ACGACAGCAT CAGCTGGGAA
    ATCCATTCTG GAGATAGAAT ACAAGCAGTA GAATGCGCTG CATGCATTAG AACCAACAAA
    TCCATGGCCA CATCCATGCT CGTGGTGAAC ACTGCCACTC AAGACTGGAA TGGCACCTAC
    ATCTGCACAT TGTCCCAGGA CTCCCTGAAC AGTTCTGCCA GCGTGACAAT CAATGTCATT
    CCGTTGCCCC TGAAGCAGCA CATCCTGATA GACCCCGCTG GAGCGCCTAT CATGTGCGGA
    ACGCCCCAAG CCCTCACCTG CTGCATCAGT GCTAACAGCA TGGGAGATTA TGATGTTAAA
    TTTGTGGTAC AAGAAAAAGA ATTTCAAGCT GAGAAAGAAG AGCAAGGAAA TTTCTTTTGC
    TACTCGTACA ATTACACAGA AACAGAATGC AGGGAAAAGA AGCTGGAGGC GTATTGCAAG
    TTTGTGAACA GCAATGAGCA GGAGGTCAGC AGCAACAGTA TAAATCTGAG CCTGATACCT
    GATAAGGACC TCATTTCCTG CTCAGAAAAC AATACCTTTG GAAAAGAAGG AGACATATTG
    AAAAAGTCGT GCTCTGTTTT AAATACTACT TTTATCCGAG GCAGCAACAT TTACAAGTGC
    TTCAACAGCT CATGGAAGAT GGAAAAGAAT GACTGCGTAT CCGAACAGAT AAACAACCTG
    CTGCTCATGG CAGAGTCCTT GGTCAACAGT CCCAGTGCAA AAGTTGGTCT ACCTAACTTC
    CTTCAGAACC TGCGGAATCA GACAGAACTG CTGCAAAATG ACAATTCTTC TGCAAATCTT
    GCTGCAATTG TTACCATCCT CTACAACATC TCTTCTATTC CAGTAGAAGC AAAGCAGTCC
    ACTATCTCTG ATTACTTCAC TACAGTGGAC ATTATCGTTA CTGATTTCAA AAAGGAACTT
    TGGGCTGACC TGAATGAAGG GCAGGTACCC ATAAGTTCTT TGTTACTGAA TGCGGTGGAA
    AGATTTTCTG AGAACCTCCA ACCAGTTAAT AATACCATTC CACATCTGTC CACTGAGACC
    CTTGAGCTGC AAGGCATGGT TGTCACAGAA CATAGCCACG CAGACTATAA TAAGAACTTC
    AGCCGTCTAG GAAACCTCAC AGCTAATGTG CTGATTGATA ACATTGAGAC TCTGCCTCCA
    AACTCAACCA TTGTCAGTGT GGCCTACTCA GCACTTGGTC ATATTTTGCC TGTGAAGGAG
    AACATGTCTG TGAACAGTTT AGTGATATCA ACAGCTCTGA GCTGCGAGAG AAACCAGAAC
    TTCCATATTA ATATGACCTT TCAGAAAGAA AACATATCTC TGAAGATGCC CCTGTGTGTC
    TTTTGGGACT TCAAACTCAA CGAAAGTAGG GGAGGCTGGA ATGATCGTGG TTGCACGCAA
    ACAGAGGAAG GCAGCAATGT CATTTGTTCC TGCAATCACT TGACACCATT CTCCATTCTG
    ATGTCACCGG ATGAACCTGC TCACAATTCT GTTGAAGATT ACATCACCCA CATTGGCCTG
    GCCATTTCAA TCTTGAGTTT AGTGGTCTGC ATTGTAATTG AATCCTTAGT TTGGAAAACT
    GTGACAAACA ACACAACCTC CTACATGCGT CACGTCTGTA TTCTCAACAC AGCGACATCT
    CTCCTAATTG CTGACATTTG GTTCATTGTT ACTGCCTCCA TCAATGTGCA GAAAAGAAAT
    ATCTGTATTG CAGCCACATT CTTCATCCAT TTATTCTACC TATGTGGCTT TTTCTGGATG
    CTCAGCCTCG GCCTCATCCT TTTTTACAGA CTAGTCTTCA TCTTACATAA CACAAGCAAG
    ACATCTCAGA AAGCAGTGGT ATTCTGCCTG GGTTATGGGT GCCCCTTTGT GTTTGCAGTC
    ACCACCATTG CTGTCACATT GCCGTGGAAC ACTTACACCA GAAAGGATGC CTGCTGGCTC
    AACTGGAAGG ACAGCAAAGC TGTCTTGGCC TTTGTCATAC CTGCCCTGGC CATCGTGGCC
    ATGAATTTGT TCATCACTGC AGTTGTGATA ATAAAAATAC TAAGGCCGAA CATTGGGGAC
    AGGACAAACA AACAAGAGAG GAAGACCTTC TTTCAAATTT GCAAAAGTCT TGTCATCTTG
    ACACCACTGT TAGGCCTCAC GTGGGGATTT GGTCTTGCCA CCATCATCAG AAATAGCCAC
    CAGACTTTCC ACATCCTCTT TGCTCTGCTC AATGCCTTGC AGGGACTGTT CATTTTGGTA
    TTTGGGACTC TATGGGACAA CAAGATAAAA GAAGCCCTGC TGAAGAGGAA TTCACTCTCC
    AGATGGAGTT CGCAACAAAC AAAGTCAACA TCCCTGATCT TGGTGACACC AATGTTTTCC
    ATGAGCTATC CATTTTCAAG AACATTCAAC AACTTATGTG GCAAAACAGG AAAATACACA
    GTATCTTCTT CAGAGCCATC CAGTGCTTCC ACAGAGAACA TCTCCAAGTC CGATTCTTTG
    CTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140652.1
    CDS416..4444
    Translation

    Target ORF information:

    RefSeq Version XM_015285166.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285166.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCCATCCC CAATTACTGC AGCTCTCCAC CTCCTGTCTC TACTGGCCGT ATGCTTCCAG 
    ACGTCTCCAG CCTCGGGCTT TGACTCATTC ACCCAATATG CCGTTGGCAG GGAGGTAGGA
    GAGGAGAGCT TCCACCCAGA GCTGCAGAGG CAGAAGCGAC ATGTGATCCT TTTTGACCTT
    CCACCCCTGG AGTATGCCAT TGACATTGAA GTAAGCCTCA CGAATTCATC CTTCCTGGCA
    CCAATCAAGG AGTACTTCAG AAACCTCCAT CTTCCAGTTT CAATCAATAT TTCAGATGTA
    GAAATGACAG TTTCAAGTAT CAACATCACA ACAGTCTGTG TGCCCAGTGA GAACAAGAGC
    TGCTGTTCTT GTGAAAGCGG ATATGCCTGG CCAAGCACGG GGTGCAGTGA CCTGATCAGC
    TGCCCTGCTC TCAGCCGAGC TCCCAACCAG CCATGCGGCT ACACCAGGGA AAGGCCTTTC
    AATGGGCCCT ACTGTGAACC TCAGAATGGA GACTCATGTG GTATGGGAGA GCCCACTGTA
    ATGAATATGT CAGTAAGACT TGACACAGCC TTCCACGATG ATCTCAGGAA TCATTCTTCC
    CAACTATACA GAAAATACAA AGCTGACCTC GATAAAGCGT TTAAAGCTAG CTACGGGTGC
    TTACCGGGCT TTGTGTCAGC AACAACAACT GGTTTCAGGC CTGGGAGTGT TGAGGTGAAC
    TACAGAGTGG AGGCAGGATC AGCGAGCTTC CAACAGCTGG AGCATTCCAG CAGATTTGTT
    ACACAGTACC TGGATGAACC ATACAGCATA GTGCCTACCT CCTTTACAGC AGAAATAACG
    AATCGCACAA ACTTCACAGT GAGCCCTGCC CACATTTTTG AAGGGGATAC CATCCAGATG
    GTGTGTGAGA TAAACACCAC GTCTGAGAAC ATGACCTGGT ATCGTGACAG TCAGATCATC
    CTCAACAGCT CACGGCACTC CGTGAGGACA GACAACAACA CTGCGATGTC ACAGGCATTT
    CTTACAATTA CCAACGTTAC GCAGGAGGAT TCTGGTACCT ACAAATGCAC TTTTGCAAAC
    AGATACCGGT CTTTAAATCT GATATACGAA GGCACCAAAG AAATACAAGT CTCTCCACTA
    CGTATCATCT CATATTCAAA CGACTATAAA GTTCACTGCA ACAGCCCTGA GATACAGGCA
    AACAGCCCTG TGCTGTTCTG TTGCATTGAT GGACAATTAC CACTGCTTAC TGGTGACTGG
    AAGGTGAATG GAGCGATCAG TATTACAGGA GTCCCCAGTT TCAGCAGCAA CTGCACTGAA
    TACAAGCTCA ACATGACGGA CTCGCTGTGC TTGGCCGAGC ATTCAGGTAC GGTGACGACA
    TACACGTGTG AGCTGCAGAC AGGGCACGGC GCCAGGAGCA GCCGGGACAT CCGAGTGATG
    TACTTCCGAG CAGCCCAGGT AAGAATATCT TCCAGCGTAA ACACAACCGT TTCCGTGGGA
    TATGGCTTCA GTCTAAAGTG TGAAAGCGAT ACGAGCAATT ACGACAGCAT CAGCTGGGAA
    ATCCATTCTG GAGATAGAAT ACAAGCAGTA GAATGCGCTG CATGCATTAG AACCAACAAA
    TCCATGGCCA CATCCATGCT CGTGGTGAAC ACTGCCACTC AAGACTGGAA TGGCACCTAC
    ATCTGCACAT TGTCCCAGGA CTCCCTGAAC AGTTCTGCCA GCGTGACAAT CAATGTCATT
    CCGTTGCCCC TGAAGCAGCA CATCCTGATA GACCCCGCTG GAGCGCCTAT CATGTGCGGA
    ACGCCCCAAG CCCTCACCTG CTGCATCAGT GCTAACAGCA TGGGAGATTA TGATGTTAAA
    TTTGTGGTAC AAGAAAAAGA ATTTCAAGCT GAGAAAGAAG AGCAAGGAAA TTTCTTTTGC
    TACTCGTACA ATTACACAGA AACAGAATGC AGGGAAAAGA AGCTGGAGGC GTATTGCAAG
    TTTGTGAACA GCAATGAGCA GGAGGTCAGC AGCAACAGTA TAAATCTGAG CCTGATACCT
    GATAAGGACC TCATTTCCTG CTCAGAAAAC AATACCTTTG GAAAAGAAGG AGACATATTG
    AAAAAGTCGT GCTCTGTTTT AAATACTACT TTTATCCGAG GCAGCAACAT TTACAAGTGC
    TTCAACAGCT CATGGAAGAT GGAAAAGAAT GACTGCGTAT CCGAACAGAT AAACAACCTG
    CTGCTCATGG CAGAGTCCTT GGTCAACAGT CCCAGTGCAA AAGTTGGTCT ACCTAACTTC
    CTTCAGAACC TGCGGAATCA GACAGAACTG CTGCAAAATG ACAATTCTTC TGCAAATCTT
    GCTGCAATTG TTACCATCCT CTACAACATC TCTTCTATTC CAGTAGAAGC AAAGCAGTCC
    ACTATCTCTG ATTACTTCAC TACAGTGGAC ATTATCGTTA CTGATTTCAA AAAGGAACTT
    TGGGCTGACC TGAATGAAGG GCAGGTACCC ATAAGTTCTT TGTTACTGAA TGCGGTGGAA
    AGATTTTCTG AGAACCTCCA ACCAGTTAAT AATACCATTC CACATCTGTC CACTGAGACC
    CTTGAGCTGC AAGGCATGGT TGTCACAGAA CATAGCCACG CAGACTATAA TAAGAACTTC
    AGCCGTCTAG GAAACCTCAC AGCTAATGTG CTGATTGATA ACATTGAGAC TCTGCCTCCA
    AACTCAACCA TTGTCAGTGT GGCCTACTCA GCACTTGGTC ATATTTTGCC TGTGAAGGAG
    AACATGTCTG TGAACAGTTT AGTGATATCA ACAGCTCTGA GCTGCGAGAG AAACCAGAAC
    TTCCATATTA ATATGACCTT TCAGAAAGAA AACATATCTC TGAAGATGCC CCTGTGTGTC
    TTTTGGGACT TCAAACTCAA CGAAAGTAGG GGAGGCTGGA ATGATCGTGG TTGCACGCAA
    ACAGAGGAAG GCAGCAATGT CATTTGTTCC TGCAATCACT TGACACCATT CTCCATTCTG
    ATGTCACCGG ATGAACCTGC TCACAATTCT GTTGAAGATT ACATCACCCA CATTGGCCTG
    GCCATTTCAA TCTTGAGTTT AGTGGTCTGC ATTGTAATTG AATCCTTAGT TTGGAAAACT
    GTGACAAACA ACACAACCTC CTACATGCGT CACGTCTGTA TTCTCAACAC AGCGACATCT
    CTCCTAATTG CTGACATTTG GTTCATTGTT ACTGCCTCCA TCAATGTGCA GAAAAGAAAT
    ATCTGTATTG CAGCCACATT CTTCATCCAT TTATTCTACC TATGTGGCTT TTTCTGGATG
    CTCAGCCTCG GCCTCATCCT TTTTTACAGA CTAGTCTTCA TCTTACATAA CACAAGCAAG
    ACATCTCAGA AAGCAGTGGT ATTCTGCCTG GGTTATGGGT GCCCCTTTGT GTTTGCAGTC
    ACCACCATTG CTGTCACATT GCCGTGGAAC ACTTACACCA GAAAGGATGC CTGCTGGCTC
    AACTGGAAGG ACAGCAAAGC TGTCTTGGCC TTTGTCATAC CTGCCCTGGC CATCGTGGCC
    ATGAATTTGT TCATCACTGC AGTTGTGATA ATAAAAATAC TAAGGCCGAA CATTGGGGAC
    AGGACAAACA AACAAGAGAG GAAGACCTTC TTTCAAATTT GCAAAAGTCT TGTCATCTTG
    ACACCACTGT TAGGCCTCAC GTGGGGATTT GGTCTTGCCA CCATCATCAG AAATAGCCAC
    CAGACTTTCC ACATCCTCTT TGCTCTGCTC AATGCCTTGC AGGGACTGTT CATTTTGGTA
    TTTGGGACTC TATGGGACAA CAAGATAAAA GAAGCCCTGC TGAAGAGGAA TTCACTCTCC
    AGATGGAGTT CGCAACAAAC AAAGTCAACA TCCCTGATCT TGGTGACACC AATGTTTTCC
    ATGAGCTATC CATTTTCAAG AACATTCAAC AACTTATGTG GCAAAACAGG AAAATACACA
    GTATCTTCTT CAGAGCCATC CAGTGCTTCC ACAGAGAACA TCTCCAAGTC CGATTCTTTG
    CTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07171
    Clone ID Related Accession (Same CDS sequence) XM_004935931.2 , XM_015285166.1 , XM_004935931.3 , XM_015285167.1 , XM_004935933.2 , XM_004935933.3 , XM_015285166.2 , XM_025149283.1 , XM_015285167.2
    Accession Version XM_025149283.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product adhesion G protein-coupled receptor F5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCCATCCC CAATTACTGC AGCTCTCCAC CTCCTGTCTC TACTGGCCGT ATGCTTCCAG 
    ACGTCTCCAG CCTCGGGCTT TGACTCATTC ACCCAATATG CCGTTGGCAG GGAGGTAGGA
    GAGGAGAGCT TCCACCCAGA GCTGCAGAGG CAGAAGCGAC ATGTGATCCT TTTTGACCTT
    CCACCCCTGG AGTATGCCAT TGACATTGAA GTAAGCCTCA CGAATTCATC CTTCCTGGCA
    CCAATCAAGG AGTACTTCAG AAACCTCCAT CTTCCAGTTT CAATCAATAT TTCAGATGTA
    GAAATGACAG TTTCAAGTAT CAACATCACA ACAGTCTGTG TGCCCAGTGA GAACAAGAGC
    TGCTGTTCTT GTGAAAGCGG ATATGCCTGG CCAAGCACGG GGTGCAGTGA CCTGATCAGC
    TGCCCTGCTC TCAGCCGAGC TCCCAACCAG CCATGCGGCT ACACCAGGGA AAGGCCTTTC
    AATGGGCCCT ACTGTGAACC TCAGAATGGA GACTCATGTG GTATGGGAGA GCCCACTGTA
    ATGAATATGT CAGTAAGACT TGACACAGCC TTCCACGATG ATCTCAGGAA TCATTCTTCC
    CAACTATACA GAAAATACAA AGCTGACCTC GATAAAGCGT TTAAAGCTAG CTACGGGTGC
    TTACCGGGCT TTGTGTCAGC AACAACAACT GGTTTCAGGC CTGGGAGTGT TGAGGTGAAC
    TACAGAGTGG AGGCAGGATC AGCGAGCTTC CAACAGCTGG AGCATTCCAG CAGATTTGTT
    ACACAGTACC TGGATGAACC ATACAGCATA GTGCCTACCT CCTTTACAGC AGAAATAACG
    AATCGCACAA ACTTCACAGT GAGCCCTGCC CACATTTTTG AAGGGGATAC CATCCAGATG
    GTGTGTGAGA TAAACACCAC GTCTGAGAAC ATGACCTGGT ATCGTGACAG TCAGATCATC
    CTCAACAGCT CACGGCACTC CGTGAGGACA GACAACAACA CTGCGATGTC ACAGGCATTT
    CTTACAATTA CCAACGTTAC GCAGGAGGAT TCTGGTACCT ACAAATGCAC TTTTGCAAAC
    AGATACCGGT CTTTAAATCT GATATACGAA GGCACCAAAG AAATACAAGT CTCTCCACTA
    CGTATCATCT CATATTCAAA CGACTATAAA GTTCACTGCA ACAGCCCTGA GATACAGGCA
    AACAGCCCTG TGCTGTTCTG TTGCATTGAT GGACAATTAC CACTGCTTAC TGGTGACTGG
    AAGGTGAATG GAGCGATCAG TATTACAGGA GTCCCCAGTT TCAGCAGCAA CTGCACTGAA
    TACAAGCTCA ACATGACGGA CTCGCTGTGC TTGGCCGAGC ATTCAGGTAC GGTGACGACA
    TACACGTGTG AGCTGCAGAC AGGGCACGGC GCCAGGAGCA GCCGGGACAT CCGAGTGATG
    TACTTCCGAG CAGCCCAGGT AAGAATATCT TCCAGCGTAA ACACAACCGT TTCCGTGGGA
    TATGGCTTCA GTCTAAAGTG TGAAAGCGAT ACGAGCAATT ACGACAGCAT CAGCTGGGAA
    ATCCATTCTG GAGATAGAAT ACAAGCAGTA GAATGCGCTG CATGCATTAG AACCAACAAA
    TCCATGGCCA CATCCATGCT CGTGGTGAAC ACTGCCACTC AAGACTGGAA TGGCACCTAC
    ATCTGCACAT TGTCCCAGGA CTCCCTGAAC AGTTCTGCCA GCGTGACAAT CAATGTCATT
    CCGTTGCCCC TGAAGCAGCA CATCCTGATA GACCCCGCTG GAGCGCCTAT CATGTGCGGA
    ACGCCCCAAG CCCTCACCTG CTGCATCAGT GCTAACAGCA TGGGAGATTA TGATGTTAAA
    TTTGTGGTAC AAGAAAAAGA ATTTCAAGCT GAGAAAGAAG AGCAAGGAAA TTTCTTTTGC
    TACTCGTACA ATTACACAGA AACAGAATGC AGGGAAAAGA AGCTGGAGGC GTATTGCAAG
    TTTGTGAACA GCAATGAGCA GGAGGTCAGC AGCAACAGTA TAAATCTGAG CCTGATACCT
    GATAAGGACC TCATTTCCTG CTCAGAAAAC AATACCTTTG GAAAAGAAGG AGACATATTG
    AAAAAGTCGT GCTCTGTTTT AAATACTACT TTTATCCGAG GCAGCAACAT TTACAAGTGC
    TTCAACAGCT CATGGAAGAT GGAAAAGAAT GACTGCGTAT CCGAACAGAT AAACAACCTG
    CTGCTCATGG CAGAGTCCTT GGTCAACAGT CCCAGTGCAA AAGTTGGTCT ACCTAACTTC
    CTTCAGAACC TGCGGAATCA GACAGAACTG CTGCAAAATG ACAATTCTTC TGCAAATCTT
    GCTGCAATTG TTACCATCCT CTACAACATC TCTTCTATTC CAGTAGAAGC AAAGCAGTCC
    ACTATCTCTG ATTACTTCAC TACAGTGGAC ATTATCGTTA CTGATTTCAA AAAGGAACTT
    TGGGCTGACC TGAATGAAGG GCAGGTACCC ATAAGTTCTT TGTTACTGAA TGCGGTGGAA
    AGATTTTCTG AGAACCTCCA ACCAGTTAAT AATACCATTC CACATCTGTC CACTGAGACC
    CTTGAGCTGC AAGGCATGGT TGTCACAGAA CATAGCCACG CAGACTATAA TAAGAACTTC
    AGCCGTCTAG GAAACCTCAC AGCTAATGTG CTGATTGATA ACATTGAGAC TCTGCCTCCA
    AACTCAACCA TTGTCAGTGT GGCCTACTCA GCACTTGGTC ATATTTTGCC TGTGAAGGAG
    AACATGTCTG TGAACAGTTT AGTGATATCA ACAGCTCTGA GCTGCGAGAG AAACCAGAAC
    TTCCATATTA ATATGACCTT TCAGAAAGAA AACATATCTC TGAAGATGCC CCTGTGTGTC
    TTTTGGGACT TCAAACTCAA CGAAAGTAGG GGAGGCTGGA ATGATCGTGG TTGCACGCAA
    ACAGAGGAAG GCAGCAATGT CATTTGTTCC TGCAATCACT TGACACCATT CTCCATTCTG
    ATGTCACCGG ATGAACCTGC TCACAATTCT GTTGAAGATT ACATCACCCA CATTGGCCTG
    GCCATTTCAA TCTTGAGTTT AGTGGTCTGC ATTGTAATTG AATCCTTAGT TTGGAAAACT
    GTGACAAACA ACACAACCTC CTACATGCGT CACGTCTGTA TTCTCAACAC AGCGACATCT
    CTCCTAATTG CTGACATTTG GTTCATTGTT ACTGCCTCCA TCAATGTGCA GAAAAGAAAT
    ATCTGTATTG CAGCCACATT CTTCATCCAT TTATTCTACC TATGTGGCTT TTTCTGGATG
    CTCAGCCTCG GCCTCATCCT TTTTTACAGA CTAGTCTTCA TCTTACATAA CACAAGCAAG
    ACATCTCAGA AAGCAGTGGT ATTCTGCCTG GGTTATGGGT GCCCCTTTGT GTTTGCAGTC
    ACCACCATTG CTGTCACATT GCCGTGGAAC ACTTACACCA GAAAGGATGC CTGCTGGCTC
    AACTGGAAGG ACAGCAAAGC TGTCTTGGCC TTTGTCATAC CTGCCCTGGC CATCGTGGCC
    ATGAATTTGT TCATCACTGC AGTTGTGATA ATAAAAATAC TAAGGCCGAA CATTGGGGAC
    AGGACAAACA AACAAGAGAG GAAGACCTTC TTTCAAATTT GCAAAAGTCT TGTCATCTTG
    ACACCACTGT TAGGCCTCAC GTGGGGATTT GGTCTTGCCA CCATCATCAG AAATAGCCAC
    CAGACTTTCC ACATCCTCTT TGCTCTGCTC AATGCCTTGC AGGGACTGTT CATTTTGGTA
    TTTGGGACTC TATGGGACAA CAAGATAAAA GAAGCCCTGC TGAAGAGGAA TTCACTCTCC
    AGATGGAGTT CGCAACAAAC AAAGTCAACA TCCCTGATCT TGGTGACACC AATGTTTTCC
    ATGAGCTATC CATTTTCAAG AACATTCAAC AACTTATGTG GCAAAACAGG AAAATACACA
    GTATCTTCTT CAGAGCCATC CAGTGCTTCC ACAGAGAACA TCTCCAAGTC CGATTCTTTG
    CTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005051.1
    CDS267..4295
    Translation

    Target ORF information:

    RefSeq Version XM_025149283.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149283.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCCATCCC CAATTACTGC AGCTCTCCAC CTCCTGTCTC TACTGGCCGT ATGCTTCCAG 
    ACGTCTCCAG CCTCGGGCTT TGACTCATTC ACCCAATATG CCGTTGGCAG GGAGGTAGGA
    GAGGAGAGCT TCCACCCAGA GCTGCAGAGG CAGAAGCGAC ATGTGATCCT TTTTGACCTT
    CCACCCCTGG AGTATGCCAT TGACATTGAA GTAAGCCTCA CGAATTCATC CTTCCTGGCA
    CCAATCAAGG AGTACTTCAG AAACCTCCAT CTTCCAGTTT CAATCAATAT TTCAGATGTA
    GAAATGACAG TTTCAAGTAT CAACATCACA ACAGTCTGTG TGCCCAGTGA GAACAAGAGC
    TGCTGTTCTT GTGAAAGCGG ATATGCCTGG CCAAGCACGG GGTGCAGTGA CCTGATCAGC
    TGCCCTGCTC TCAGCCGAGC TCCCAACCAG CCATGCGGCT ACACCAGGGA AAGGCCTTTC
    AATGGGCCCT ACTGTGAACC TCAGAATGGA GACTCATGTG GTATGGGAGA GCCCACTGTA
    ATGAATATGT CAGTAAGACT TGACACAGCC TTCCACGATG ATCTCAGGAA TCATTCTTCC
    CAACTATACA GAAAATACAA AGCTGACCTC GATAAAGCGT TTAAAGCTAG CTACGGGTGC
    TTACCGGGCT TTGTGTCAGC AACAACAACT GGTTTCAGGC CTGGGAGTGT TGAGGTGAAC
    TACAGAGTGG AGGCAGGATC AGCGAGCTTC CAACAGCTGG AGCATTCCAG CAGATTTGTT
    ACACAGTACC TGGATGAACC ATACAGCATA GTGCCTACCT CCTTTACAGC AGAAATAACG
    AATCGCACAA ACTTCACAGT GAGCCCTGCC CACATTTTTG AAGGGGATAC CATCCAGATG
    GTGTGTGAGA TAAACACCAC GTCTGAGAAC ATGACCTGGT ATCGTGACAG TCAGATCATC
    CTCAACAGCT CACGGCACTC CGTGAGGACA GACAACAACA CTGCGATGTC ACAGGCATTT
    CTTACAATTA CCAACGTTAC GCAGGAGGAT TCTGGTACCT ACAAATGCAC TTTTGCAAAC
    AGATACCGGT CTTTAAATCT GATATACGAA GGCACCAAAG AAATACAAGT CTCTCCACTA
    CGTATCATCT CATATTCAAA CGACTATAAA GTTCACTGCA ACAGCCCTGA GATACAGGCA
    AACAGCCCTG TGCTGTTCTG TTGCATTGAT GGACAATTAC CACTGCTTAC TGGTGACTGG
    AAGGTGAATG GAGCGATCAG TATTACAGGA GTCCCCAGTT TCAGCAGCAA CTGCACTGAA
    TACAAGCTCA ACATGACGGA CTCGCTGTGC TTGGCCGAGC ATTCAGGTAC GGTGACGACA
    TACACGTGTG AGCTGCAGAC AGGGCACGGC GCCAGGAGCA GCCGGGACAT CCGAGTGATG
    TACTTCCGAG CAGCCCAGGT AAGAATATCT TCCAGCGTAA ACACAACCGT TTCCGTGGGA
    TATGGCTTCA GTCTAAAGTG TGAAAGCGAT ACGAGCAATT ACGACAGCAT CAGCTGGGAA
    ATCCATTCTG GAGATAGAAT ACAAGCAGTA GAATGCGCTG CATGCATTAG AACCAACAAA
    TCCATGGCCA CATCCATGCT CGTGGTGAAC ACTGCCACTC AAGACTGGAA TGGCACCTAC
    ATCTGCACAT TGTCCCAGGA CTCCCTGAAC AGTTCTGCCA GCGTGACAAT CAATGTCATT
    CCGTTGCCCC TGAAGCAGCA CATCCTGATA GACCCCGCTG GAGCGCCTAT CATGTGCGGA
    ACGCCCCAAG CCCTCACCTG CTGCATCAGT GCTAACAGCA TGGGAGATTA TGATGTTAAA
    TTTGTGGTAC AAGAAAAAGA ATTTCAAGCT GAGAAAGAAG AGCAAGGAAA TTTCTTTTGC
    TACTCGTACA ATTACACAGA AACAGAATGC AGGGAAAAGA AGCTGGAGGC GTATTGCAAG
    TTTGTGAACA GCAATGAGCA GGAGGTCAGC AGCAACAGTA TAAATCTGAG CCTGATACCT
    GATAAGGACC TCATTTCCTG CTCAGAAAAC AATACCTTTG GAAAAGAAGG AGACATATTG
    AAAAAGTCGT GCTCTGTTTT AAATACTACT TTTATCCGAG GCAGCAACAT TTACAAGTGC
    TTCAACAGCT CATGGAAGAT GGAAAAGAAT GACTGCGTAT CCGAACAGAT AAACAACCTG
    CTGCTCATGG CAGAGTCCTT GGTCAACAGT CCCAGTGCAA AAGTTGGTCT ACCTAACTTC
    CTTCAGAACC TGCGGAATCA GACAGAACTG CTGCAAAATG ACAATTCTTC TGCAAATCTT
    GCTGCAATTG TTACCATCCT CTACAACATC TCTTCTATTC CAGTAGAAGC AAAGCAGTCC
    ACTATCTCTG ATTACTTCAC TACAGTGGAC ATTATCGTTA CTGATTTCAA AAAGGAACTT
    TGGGCTGACC TGAATGAAGG GCAGGTACCC ATAAGTTCTT TGTTACTGAA TGCGGTGGAA
    AGATTTTCTG AGAACCTCCA ACCAGTTAAT AATACCATTC CACATCTGTC CACTGAGACC
    CTTGAGCTGC AAGGCATGGT TGTCACAGAA CATAGCCACG CAGACTATAA TAAGAACTTC
    AGCCGTCTAG GAAACCTCAC AGCTAATGTG CTGATTGATA ACATTGAGAC TCTGCCTCCA
    AACTCAACCA TTGTCAGTGT GGCCTACTCA GCACTTGGTC ATATTTTGCC TGTGAAGGAG
    AACATGTCTG TGAACAGTTT AGTGATATCA ACAGCTCTGA GCTGCGAGAG AAACCAGAAC
    TTCCATATTA ATATGACCTT TCAGAAAGAA AACATATCTC TGAAGATGCC CCTGTGTGTC
    TTTTGGGACT TCAAACTCAA CGAAAGTAGG GGAGGCTGGA ATGATCGTGG TTGCACGCAA
    ACAGAGGAAG GCAGCAATGT CATTTGTTCC TGCAATCACT TGACACCATT CTCCATTCTG
    ATGTCACCGG ATGAACCTGC TCACAATTCT GTTGAAGATT ACATCACCCA CATTGGCCTG
    GCCATTTCAA TCTTGAGTTT AGTGGTCTGC ATTGTAATTG AATCCTTAGT TTGGAAAACT
    GTGACAAACA ACACAACCTC CTACATGCGT CACGTCTGTA TTCTCAACAC AGCGACATCT
    CTCCTAATTG CTGACATTTG GTTCATTGTT ACTGCCTCCA TCAATGTGCA GAAAAGAAAT
    ATCTGTATTG CAGCCACATT CTTCATCCAT TTATTCTACC TATGTGGCTT TTTCTGGATG
    CTCAGCCTCG GCCTCATCCT TTTTTACAGA CTAGTCTTCA TCTTACATAA CACAAGCAAG
    ACATCTCAGA AAGCAGTGGT ATTCTGCCTG GGTTATGGGT GCCCCTTTGT GTTTGCAGTC
    ACCACCATTG CTGTCACATT GCCGTGGAAC ACTTACACCA GAAAGGATGC CTGCTGGCTC
    AACTGGAAGG ACAGCAAAGC TGTCTTGGCC TTTGTCATAC CTGCCCTGGC CATCGTGGCC
    ATGAATTTGT TCATCACTGC AGTTGTGATA ATAAAAATAC TAAGGCCGAA CATTGGGGAC
    AGGACAAACA AACAAGAGAG GAAGACCTTC TTTCAAATTT GCAAAAGTCT TGTCATCTTG
    ACACCACTGT TAGGCCTCAC GTGGGGATTT GGTCTTGCCA CCATCATCAG AAATAGCCAC
    CAGACTTTCC ACATCCTCTT TGCTCTGCTC AATGCCTTGC AGGGACTGTT CATTTTGGTA
    TTTGGGACTC TATGGGACAA CAAGATAAAA GAAGCCCTGC TGAAGAGGAA TTCACTCTCC
    AGATGGAGTT CGCAACAAAC AAAGTCAACA TCCCTGATCT TGGTGACACC AATGTTTTCC
    ATGAGCTATC CATTTTCAAG AACATTCAAC AACTTATGTG GCAAAACAGG AAAATACACA
    GTATCTTCTT CAGAGCCATC CAGTGCTTCC ACAGAGAACA TCTCCAAGTC CGATTCTTTG
    CTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07171
    Clone ID Related Accession (Same CDS sequence) XM_004935931.2 , XM_015285166.1 , XM_004935931.3 , XM_015285167.1 , XM_004935933.2 , XM_004935933.3 , XM_015285166.2 , XM_025149283.1 , XM_015285167.2
    Accession Version XM_015285167.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product adhesion G protein-coupled receptor F5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285167.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCCATCCC CAATTACTGC AGCTCTCCAC CTCCTGTCTC TACTGGCCGT ATGCTTCCAG 
    ACGTCTCCAG CCTCGGGCTT TGACTCATTC ACCCAATATG CCGTTGGCAG GGAGGTAGGA
    GAGGAGAGCT TCCACCCAGA GCTGCAGAGG CAGAAGCGAC ATGTGATCCT TTTTGACCTT
    CCACCCCTGG AGTATGCCAT TGACATTGAA GTAAGCCTCA CGAATTCATC CTTCCTGGCA
    CCAATCAAGG AGTACTTCAG AAACCTCCAT CTTCCAGTTT CAATCAATAT TTCAGATGTA
    GAAATGACAG TTTCAAGTAT CAACATCACA ACAGTCTGTG TGCCCAGTGA GAACAAGAGC
    TGCTGTTCTT GTGAAAGCGG ATATGCCTGG CCAAGCACGG GGTGCAGTGA CCTGATCAGC
    TGCCCTGCTC TCAGCCGAGC TCCCAACCAG CCATGCGGCT ACACCAGGGA AAGGCCTTTC
    AATGGGCCCT ACTGTGAACC TCAGAATGGA GACTCATGTG GTATGGGAGA GCCCACTGTA
    ATGAATATGT CAGTAAGACT TGACACAGCC TTCCACGATG ATCTCAGGAA TCATTCTTCC
    CAACTATACA GAAAATACAA AGCTGACCTC GATAAAGCGT TTAAAGCTAG CTACGGGTGC
    TTACCGGGCT TTGTGTCAGC AACAACAACT GGTTTCAGGC CTGGGAGTGT TGAGGTGAAC
    TACAGAGTGG AGGCAGGATC AGCGAGCTTC CAACAGCTGG AGCATTCCAG CAGATTTGTT
    ACACAGTACC TGGATGAACC ATACAGCATA GTGCCTACCT CCTTTACAGC AGAAATAACG
    AATCGCACAA ACTTCACAGT GAGCCCTGCC CACATTTTTG AAGGGGATAC CATCCAGATG
    GTGTGTGAGA TAAACACCAC GTCTGAGAAC ATGACCTGGT ATCGTGACAG TCAGATCATC
    CTCAACAGCT CACGGCACTC CGTGAGGACA GACAACAACA CTGCGATGTC ACAGGCATTT
    CTTACAATTA CCAACGTTAC GCAGGAGGAT TCTGGTACCT ACAAATGCAC TTTTGCAAAC
    AGATACCGGT CTTTAAATCT GATATACGAA GGCACCAAAG AAATACAAGT CTCTCCACTA
    CGTATCATCT CATATTCAAA CGACTATAAA GTTCACTGCA ACAGCCCTGA GATACAGGCA
    AACAGCCCTG TGCTGTTCTG TTGCATTGAT GGACAATTAC CACTGCTTAC TGGTGACTGG
    AAGGTGAATG GAGCGATCAG TATTACAGGA GTCCCCAGTT TCAGCAGCAA CTGCACTGAA
    TACAAGCTCA ACATGACGGA CTCGCTGTGC TTGGCCGAGC ATTCAGGTAC GGTGACGACA
    TACACGTGTG AGCTGCAGAC AGGGCACGGC GCCAGGAGCA GCCGGGACAT CCGAGTGATG
    TACTTCCGAG CAGCCCAGGT AAGAATATCT TCCAGCGTAA ACACAACCGT TTCCGTGGGA
    TATGGCTTCA GTCTAAAGTG TGAAAGCGAT ACGAGCAATT ACGACAGCAT CAGCTGGGAA
    ATCCATTCTG GAGATAGAAT ACAAGCAGTA GAATGCGCTG CATGCATTAG AACCAACAAA
    TCCATGGCCA CATCCATGCT CGTGGTGAAC ACTGCCACTC AAGACTGGAA TGGCACCTAC
    ATCTGCACAT TGTCCCAGGA CTCCCTGAAC AGTTCTGCCA GCGTGACAAT CAATGTCATT
    CCGTTGCCCC TGAAGCAGCA CATCCTGATA GACCCCGCTG GAGCGCCTAT CATGTGCGGA
    ACGCCCCAAG CCCTCACCTG CTGCATCAGT GCTAACAGCA TGGGAGATTA TGATGTTAAA
    TTTGTGGTAC AAGAAAAAGA ATTTCAAGCT GAGAAAGAAG AGCAAGGAAA TTTCTTTTGC
    TACTCGTACA ATTACACAGA AACAGAATGC AGGGAAAAGA AGCTGGAGGC GTATTGCAAG
    TTTGTGAACA GCAATGAGCA GGAGGTCAGC AGCAACAGTA TAAATCTGAG CCTGATACCT
    GATAAGGACC TCATTTCCTG CTCAGAAAAC AATACCTTTG GAAAAGAAGG AGACATATTG
    AAAAAGTCGT GCTCTGTTTT AAATACTACT TTTATCCGAG GCAGCAACAT TTACAAGTGC
    TTCAACAGCT CATGGAAGAT GGAAAAGAAT GACTGCGTAT CCGAACAGAT AAACAACCTG
    CTGCTCATGG CAGAGTCCTT GGTCAACAGT CCCAGTGCAA AAGTTGGTCT ACCTAACTTC
    CTTCAGAACC TGCGGAATCA GACAGAACTG CTGCAAAATG ACAATTCTTC TGCAAATCTT
    GCTGCAATTG TTACCATCCT CTACAACATC TCTTCTATTC CAGTAGAAGC AAAGCAGTCC
    ACTATCTCTG ATTACTTCAC TACAGTGGAC ATTATCGTTA CTGATTTCAA AAAGGAACTT
    TGGGCTGACC TGAATGAAGG GCAGGTACCC ATAAGTTCTT TGTTACTGAA TGCGGTGGAA
    AGATTTTCTG AGAACCTCCA ACCAGTTAAT AATACCATTC CACATCTGTC CACTGAGACC
    CTTGAGCTGC AAGGCATGGT TGTCACAGAA CATAGCCACG CAGACTATAA TAAGAACTTC
    AGCCGTCTAG GAAACCTCAC AGCTAATGTG CTGATTGATA ACATTGAGAC TCTGCCTCCA
    AACTCAACCA TTGTCAGTGT GGCCTACTCA GCACTTGGTC ATATTTTGCC TGTGAAGGAG
    AACATGTCTG TGAACAGTTT AGTGATATCA ACAGCTCTGA GCTGCGAGAG AAACCAGAAC
    TTCCATATTA ATATGACCTT TCAGAAAGAA AACATATCTC TGAAGATGCC CCTGTGTGTC
    TTTTGGGACT TCAAACTCAA CGAAAGTAGG GGAGGCTGGA ATGATCGTGG TTGCACGCAA
    ACAGAGGAAG GCAGCAATGT CATTTGTTCC TGCAATCACT TGACACCATT CTCCATTCTG
    ATGTCACCGG ATGAACCTGC TCACAATTCT GTTGAAGATT ACATCACCCA CATTGGCCTG
    GCCATTTCAA TCTTGAGTTT AGTGGTCTGC ATTGTAATTG AATCCTTAGT TTGGAAAACT
    GTGACAAACA ACACAACCTC CTACATGCGT CACGTCTGTA TTCTCAACAC AGCGACATCT
    CTCCTAATTG CTGACATTTG GTTCATTGTT ACTGCCTCCA TCAATGTGCA GAAAAGAAAT
    ATCTGTATTG CAGCCACATT CTTCATCCAT TTATTCTACC TATGTGGCTT TTTCTGGATG
    CTCAGCCTCG GCCTCATCCT TTTTTACAGA CTAGTCTTCA TCTTACATAA CACAAGCAAG
    ACATCTCAGA AAGCAGTGGT ATTCTGCCTG GGTTATGGGT GCCCCTTTGT GTTTGCAGTC
    ACCACCATTG CTGTCACATT GCCGTGGAAC ACTTACACCA GAAAGGATGC CTGCTGGCTC
    AACTGGAAGG ACAGCAAAGC TGTCTTGGCC TTTGTCATAC CTGCCCTGGC CATCGTGGCC
    ATGAATTTGT TCATCACTGC AGTTGTGATA ATAAAAATAC TAAGGCCGAA CATTGGGGAC
    AGGACAAACA AACAAGAGAG GAAGACCTTC TTTCAAATTT GCAAAAGTCT TGTCATCTTG
    ACACCACTGT TAGGCCTCAC GTGGGGATTT GGTCTTGCCA CCATCATCAG AAATAGCCAC
    CAGACTTTCC ACATCCTCTT TGCTCTGCTC AATGCCTTGC AGGGACTGTT CATTTTGGTA
    TTTGGGACTC TATGGGACAA CAAGATAAAA GAAGCCCTGC TGAAGAGGAA TTCACTCTCC
    AGATGGAGTT CGCAACAAAC AAAGTCAACA TCCCTGATCT TGGTGACACC AATGTTTTCC
    ATGAGCTATC CATTTTCAAG AACATTCAAC AACTTATGTG GCAAAACAGG AAAATACACA
    GTATCTTCTT CAGAGCCATC CAGTGCTTCC ACAGAGAACA TCTCCAAGTC CGATTCTTTG
    CTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140653.1
    CDS248..4276
    Translation

    Target ORF information:

    RefSeq Version XM_015285167.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285167.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCCATCCC CAATTACTGC AGCTCTCCAC CTCCTGTCTC TACTGGCCGT ATGCTTCCAG 
    ACGTCTCCAG CCTCGGGCTT TGACTCATTC ACCCAATATG CCGTTGGCAG GGAGGTAGGA
    GAGGAGAGCT TCCACCCAGA GCTGCAGAGG CAGAAGCGAC ATGTGATCCT TTTTGACCTT
    CCACCCCTGG AGTATGCCAT TGACATTGAA GTAAGCCTCA CGAATTCATC CTTCCTGGCA
    CCAATCAAGG AGTACTTCAG AAACCTCCAT CTTCCAGTTT CAATCAATAT TTCAGATGTA
    GAAATGACAG TTTCAAGTAT CAACATCACA ACAGTCTGTG TGCCCAGTGA GAACAAGAGC
    TGCTGTTCTT GTGAAAGCGG ATATGCCTGG CCAAGCACGG GGTGCAGTGA CCTGATCAGC
    TGCCCTGCTC TCAGCCGAGC TCCCAACCAG CCATGCGGCT ACACCAGGGA AAGGCCTTTC
    AATGGGCCCT ACTGTGAACC TCAGAATGGA GACTCATGTG GTATGGGAGA GCCCACTGTA
    ATGAATATGT CAGTAAGACT TGACACAGCC TTCCACGATG ATCTCAGGAA TCATTCTTCC
    CAACTATACA GAAAATACAA AGCTGACCTC GATAAAGCGT TTAAAGCTAG CTACGGGTGC
    TTACCGGGCT TTGTGTCAGC AACAACAACT GGTTTCAGGC CTGGGAGTGT TGAGGTGAAC
    TACAGAGTGG AGGCAGGATC AGCGAGCTTC CAACAGCTGG AGCATTCCAG CAGATTTGTT
    ACACAGTACC TGGATGAACC ATACAGCATA GTGCCTACCT CCTTTACAGC AGAAATAACG
    AATCGCACAA ACTTCACAGT GAGCCCTGCC CACATTTTTG AAGGGGATAC CATCCAGATG
    GTGTGTGAGA TAAACACCAC GTCTGAGAAC ATGACCTGGT ATCGTGACAG TCAGATCATC
    CTCAACAGCT CACGGCACTC CGTGAGGACA GACAACAACA CTGCGATGTC ACAGGCATTT
    CTTACAATTA CCAACGTTAC GCAGGAGGAT TCTGGTACCT ACAAATGCAC TTTTGCAAAC
    AGATACCGGT CTTTAAATCT GATATACGAA GGCACCAAAG AAATACAAGT CTCTCCACTA
    CGTATCATCT CATATTCAAA CGACTATAAA GTTCACTGCA ACAGCCCTGA GATACAGGCA
    AACAGCCCTG TGCTGTTCTG TTGCATTGAT GGACAATTAC CACTGCTTAC TGGTGACTGG
    AAGGTGAATG GAGCGATCAG TATTACAGGA GTCCCCAGTT TCAGCAGCAA CTGCACTGAA
    TACAAGCTCA ACATGACGGA CTCGCTGTGC TTGGCCGAGC ATTCAGGTAC GGTGACGACA
    TACACGTGTG AGCTGCAGAC AGGGCACGGC GCCAGGAGCA GCCGGGACAT CCGAGTGATG
    TACTTCCGAG CAGCCCAGGT AAGAATATCT TCCAGCGTAA ACACAACCGT TTCCGTGGGA
    TATGGCTTCA GTCTAAAGTG TGAAAGCGAT ACGAGCAATT ACGACAGCAT CAGCTGGGAA
    ATCCATTCTG GAGATAGAAT ACAAGCAGTA GAATGCGCTG CATGCATTAG AACCAACAAA
    TCCATGGCCA CATCCATGCT CGTGGTGAAC ACTGCCACTC AAGACTGGAA TGGCACCTAC
    ATCTGCACAT TGTCCCAGGA CTCCCTGAAC AGTTCTGCCA GCGTGACAAT CAATGTCATT
    CCGTTGCCCC TGAAGCAGCA CATCCTGATA GACCCCGCTG GAGCGCCTAT CATGTGCGGA
    ACGCCCCAAG CCCTCACCTG CTGCATCAGT GCTAACAGCA TGGGAGATTA TGATGTTAAA
    TTTGTGGTAC AAGAAAAAGA ATTTCAAGCT GAGAAAGAAG AGCAAGGAAA TTTCTTTTGC
    TACTCGTACA ATTACACAGA AACAGAATGC AGGGAAAAGA AGCTGGAGGC GTATTGCAAG
    TTTGTGAACA GCAATGAGCA GGAGGTCAGC AGCAACAGTA TAAATCTGAG CCTGATACCT
    GATAAGGACC TCATTTCCTG CTCAGAAAAC AATACCTTTG GAAAAGAAGG AGACATATTG
    AAAAAGTCGT GCTCTGTTTT AAATACTACT TTTATCCGAG GCAGCAACAT TTACAAGTGC
    TTCAACAGCT CATGGAAGAT GGAAAAGAAT GACTGCGTAT CCGAACAGAT AAACAACCTG
    CTGCTCATGG CAGAGTCCTT GGTCAACAGT CCCAGTGCAA AAGTTGGTCT ACCTAACTTC
    CTTCAGAACC TGCGGAATCA GACAGAACTG CTGCAAAATG ACAATTCTTC TGCAAATCTT
    GCTGCAATTG TTACCATCCT CTACAACATC TCTTCTATTC CAGTAGAAGC AAAGCAGTCC
    ACTATCTCTG ATTACTTCAC TACAGTGGAC ATTATCGTTA CTGATTTCAA AAAGGAACTT
    TGGGCTGACC TGAATGAAGG GCAGGTACCC ATAAGTTCTT TGTTACTGAA TGCGGTGGAA
    AGATTTTCTG AGAACCTCCA ACCAGTTAAT AATACCATTC CACATCTGTC CACTGAGACC
    CTTGAGCTGC AAGGCATGGT TGTCACAGAA CATAGCCACG CAGACTATAA TAAGAACTTC
    AGCCGTCTAG GAAACCTCAC AGCTAATGTG CTGATTGATA ACATTGAGAC TCTGCCTCCA
    AACTCAACCA TTGTCAGTGT GGCCTACTCA GCACTTGGTC ATATTTTGCC TGTGAAGGAG
    AACATGTCTG TGAACAGTTT AGTGATATCA ACAGCTCTGA GCTGCGAGAG AAACCAGAAC
    TTCCATATTA ATATGACCTT TCAGAAAGAA AACATATCTC TGAAGATGCC CCTGTGTGTC
    TTTTGGGACT TCAAACTCAA CGAAAGTAGG GGAGGCTGGA ATGATCGTGG TTGCACGCAA
    ACAGAGGAAG GCAGCAATGT CATTTGTTCC TGCAATCACT TGACACCATT CTCCATTCTG
    ATGTCACCGG ATGAACCTGC TCACAATTCT GTTGAAGATT ACATCACCCA CATTGGCCTG
    GCCATTTCAA TCTTGAGTTT AGTGGTCTGC ATTGTAATTG AATCCTTAGT TTGGAAAACT
    GTGACAAACA ACACAACCTC CTACATGCGT CACGTCTGTA TTCTCAACAC AGCGACATCT
    CTCCTAATTG CTGACATTTG GTTCATTGTT ACTGCCTCCA TCAATGTGCA GAAAAGAAAT
    ATCTGTATTG CAGCCACATT CTTCATCCAT TTATTCTACC TATGTGGCTT TTTCTGGATG
    CTCAGCCTCG GCCTCATCCT TTTTTACAGA CTAGTCTTCA TCTTACATAA CACAAGCAAG
    ACATCTCAGA AAGCAGTGGT ATTCTGCCTG GGTTATGGGT GCCCCTTTGT GTTTGCAGTC
    ACCACCATTG CTGTCACATT GCCGTGGAAC ACTTACACCA GAAAGGATGC CTGCTGGCTC
    AACTGGAAGG ACAGCAAAGC TGTCTTGGCC TTTGTCATAC CTGCCCTGGC CATCGTGGCC
    ATGAATTTGT TCATCACTGC AGTTGTGATA ATAAAAATAC TAAGGCCGAA CATTGGGGAC
    AGGACAAACA AACAAGAGAG GAAGACCTTC TTTCAAATTT GCAAAAGTCT TGTCATCTTG
    ACACCACTGT TAGGCCTCAC GTGGGGATTT GGTCTTGCCA CCATCATCAG AAATAGCCAC
    CAGACTTTCC ACATCCTCTT TGCTCTGCTC AATGCCTTGC AGGGACTGTT CATTTTGGTA
    TTTGGGACTC TATGGGACAA CAAGATAAAA GAAGCCCTGC TGAAGAGGAA TTCACTCTCC
    AGATGGAGTT CGCAACAAAC AAAGTCAACA TCCCTGATCT TGGTGACACC AATGTTTTCC
    ATGAGCTATC CATTTTCAAG AACATTCAAC AACTTATGTG GCAAAACAGG AAAATACACA
    GTATCTTCTT CAGAGCCATC CAGTGCTTCC ACAGAGAACA TCTCCAAGTC CGATTCTTTG
    CTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.