ERN1 cDNA ORF clone, Galeopterus variegatus(Sunda flying lemur)

Gene
Clones
gRNAs

Summary

Gene Symbol	ERN1
Entrez Gene ID	103603263
Full Name	endoplasmic reticulum to nucleus signaling 1
Gene Type	protein-coding
Organism	Galeopterus variegatus(Sunda flying lemur)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008587754.1	XP_008585976.1	serine/threonine-protein kinase/endoribonuclease IRE1

Pathways from BioSystems
Gene Ontology

The following ERN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ERN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa113779	XM_008587754.1 Latest version!	Galeopterus variegatus endoplasmic reticulum to nucleus signaling 1 (ERN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ERN1 ( XM_008587754.1 ) cDNA ORF clone, Galeopterus variegatus(Sunda flying lemur) -> XP_008585976.1 Galeopterus variegatus endoplasmic reticulum to nucleus signaling 1 (ERN1), mRNA.

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CloneID	OGa113779
Clone ID Related Accession (Same CDS sequence)	XM_008587754.1
Accession Version	XM_008587754.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-07-21
Organism	Galeopterus variegatus(Sunda flying lemur)
Product	serine/threonine-protein kinase/endoribonuclease IRE1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007730636.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Galeopterus variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 17% of CDS bases ##RefSeq-Attributes-END##

ATGAGGCACA ACCGGGGGTG TGAACTCTGC GACTGCCCTG CTCAGGGCAG AGACTCCGTG 
TTTGCTGGAA ACCCCTGCCA GCTGCTGAAA CCATCGCGCC AAAGGGCGTC TCAGTCAACC 
TCGCCCATGA CTGCCCCACG GCGGGAAGAC GAAAAGGGGG GTTTCTGGGC ACCCGGTACA 
GCCCGTGTCC CGGGTCCCTC GCCTGGGGGG TACGGGTTGT CGGTCTTGCC CAGCCTCGGA 
CGAGCTTCTT ACCTAGACCC TTCCCCGCTC CTGGACGCCG GACGCCGAGG CCTGGTCCTG 
CTCCTCAGCC GCCGCGTCCG CAGGACCCGA GCCAGTCAGA GGCCCCGCTC AGGCGTCCGC 
TGCCCCAGTG GCCTCCCGGG CCAGCGCGGC TGTCACGGGC TCGGGCGCAA CTCTGTGGCC 
CTGGGCGGCA CCTCGGTATC TCCAGAAGGC CCGGGCCTTC GGGAGGCCGC CCACCTGCCG 
GACCCTCCCC GCAGTGAGAA CCAACAGCAA GGCGAAGGGC TGAACCTCTC CCCCTCCAGG 
CCAGGTACAC CCAAGAGATG GGATGAGCGA GGTTCTGACA GCTCCTTCGC CCTCCTCTGC 
CAGTGGATTT TTGGAAGCAC CAGCACAGTG ACGCTTCCTG AAACCTTGCT GTTTGTGTCA 
ACCCTGGATG GCAGTTTGCA TGCTGTCAGC AAGAGGACAG GCTCAATCAA ATGGACATTA 
AAAGAAGATC CAGTCCTGCA GGTCCCAACA CATGTGGAAG AGCCTGCCTT CCTTCCAGAT 
CCCAATGATG GCAGCCTGTA TACGCTTGGA GGCAAGAATA ATGAAGGCCT GACAAAACTT 
CCCTTTACTA TCCCAGAGTT GGTACAGGCA TCCCCTTGCC GAAGTTCAGA TGGAATTCTG 
TACATGGGTA AAAAGCAGGA CATTTGGTAT GTTATCGACC TCCTGACCGG GGAGAAGCAG 
CAGACTTTGT CATCAGCCTT TGCAGACAGT CTCTGCCCAT CAACCTCTCT TCTGTATCTT 
GGGCGAACAG AATACACCAT CACTATGTAT GACACCAAAA CCCGAGAGCT CCGGTGGAAC 
GCCACCTACT TTGACTACGC AGCCTCACTG CCTGAGGACG ACATGGACTA CAAGATGTCC 
CACTTTGTGT CCAATGGTGA TGGGCTGGTG GTGACTGTGG ACAGTGAATC TGGGGACGTC 
CTGTGGATCC AAAACTATGC CTCCCCTGTG GTGGCCTTTT ATGTCTGGCA GCGGGAGGGT 
CTGAGGAAGG TGATGCACAT CAACGTTGCT GTGGAGACCC TGCGCTATCT GACCTTCATG 
TCTGGGGAGG TGGGACGCAT CACCAAGTGG AAGTACCCGT TCCCCAAGGA GACGGAGGCC 
AAGAGCAAGC TGATGCCCAC CCTGTATGTT GGGAAATACT CTACCAGCCT CTACGCCTCC 
CCCTCCATGG TACATGAAGG TGTCGCTGTC GTGCCCCGCG GCAGCACGCT TCCTTTGCTG 
GAAGGCCCCC AGACTGATGG TGTCACCATT GGGGACAAAG GAGAGTGTGT GATCACCCCC 
AGCACGGACC TCAAGTTCGA CCCTGGACTC AAGGGGAAGA ACAAGCTCCA TGACTTGAGG 
AACTACTGGC TTCTGATAGG ACACCATGAA ACCCCACTGT CTGCGTCTAC CAAGATGCTG 
GAGAGATTTC CCAACAATCT GCCGAAACAT CGGGAAAATG TGATTCCTGC TGATTCGGAG 
AAGAACTTTG AGGAGGTTAT CAACCTGGTT GACGAGACTT CGGAAAACGC GCCTACCACC 
GTGTCCCAGG GGGTGGGGGA GAAGTTTGCT CATGCACCTG CCAAGCCCGA GGCCCCCATG 
GACTCCATGC TCAAGGACGT GGCCACCATC ATCCTGAGCA CCTTCCTGCT CATCGGCTGG 
GTGGCCTTCA TCATCACCTA CCCCTTGAGC ATGCATCAGC AGCAGCAGCA CCAGCAGTTC 
CAGAAGGAAC TGGAGAAAAT CCAGCTCCTG CAACAGCAGC AGCTGCCCTT CCATCCAGCT 
GGAGATGCGA CTCAGGACAC CGAGCTCTTG GACACATCTG GGCCGTACTC GGAGAGCTCG 
GGCACCAGCA GCCCCAGCAT GTCCCCCCGA GCCTCCAACC ACTCACTCCA ATCTGCCTCT 
AAGGCTGGCA CAAGCCTGTC CCTGGAGCAG GATGACGACG ATGAGGAAAC CTGCATGGTG 
ATAGTTGGGA AAATTTCATT TTGTCCCAAA GACGTCCTGG GTCACGGAGC TGAGGGCACC 
ATTGTGTACC GGGGCATGTT TGACAACCGC GATGTGGCCG TGAAGAGGAT CCTCCCTGAG 
TGTTTTAGCT TTGCAGACCG TGAGGTCCAG CTGCTGCGAG AGTCAGACGA GCACCCAAAC 
GTGATCCGTT ACTTCTGCAC GGAGAGGGAC CGGCAGTTCC AGTACATTGC CATCGAGCTG 
TGCGCAGCCA CCTTGCAAGA GTACGTGGAG CAGAAGGACT TTGCCCACCT CGGCCTGGAG 
CCCATCACCT TGCTACAGCA GACCACCTCA GGCCTGGCCC ACCTGCACTC CCTCAACATT 
GTTCACAGAG ACCTGAAGCC CCACAACATT CTCCTCTCCA TGCCCAATGC CCATGGCAGG 
ATCAAGGCCA TGATCTCTGA CTTTGGCCTC TGCAAGAAGC TGGCAGTGGG CAGGCACAGC 
TTCAGCCGCC GTTCTGGGGT GCCCGGCACA GAAGGCTGGA TTGCTCCGGA GATGCTGAGT 
GAAGACTGCA AGGAGAACCC TACCTACACG GTGGACATCT TCTCCGCGGG CTGCGTCTTT 
TACTACGTGA TCTCTGAGGG CAACCACCCT TTCGGCAAGT CCCTGCAGCG GCAGGCTAAC 
ATCCTCCTGG GTGCCTACAG CCTCGACTGC TTCCACCCAG AGAAGCACGA AGACATCATT 
GCACGTGAAT TAATAGAGAA AATGATTGCC ATGGATCCTC AGAAACGCCC CTCAGCAAAG 
CACGTGCTAA AGCACCCGTT CTTCTGGAGC CTGGAGAAGC AGCTGCAGTT CTTTCAGGAT 
GTGAGTGACC GAATAGAAAA GGAGTCCCTG GATGGCCCAA TCGTGAAGCA GTTGGAGAGA 
GGCGGGAGAG CCGTGGTGAA GATGGACTGG CGGGAGAACA TCACAGTCCC CCTCCAGACA 
GATCTGCGTA AATTCAGAAC CTATAAAGGT GGCTCAGTCA GAGATCTCCT CCGAGCCATG 
AGAAACAAGA GGCACCACTA CCGGGAACTC CCCACGGAGG TGCGGGAGAC GCTGGGCGCG 
CTGCCCGACG GCTTCGTGCG CTACTTCACC TCACGCTTCC CCCACCTGCT CTTGCACACC 
TACTGGGCCA TGGAGCTGTG CGGCCACGAG AGACTCTTCC AGCCCTACTA CTGCCACGAG 
CCCCCGGAGC CCTGGCCCAC AGTGACTCCA GATGCCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008585976.1
CDS	1..3462
Translation	MRHNRGCELCDCPAQGRDSVFAGNPCQLLKPSRQRASQSTSPMTAPRREDEKGGFWAPGTARVPGPSPGGYGLSVLPSLGRASYLDPSPLLDAGRRGLVLLLSRRVRRTRASQRPRSGVRCPSGLPGQRGCHGLGRNSVALGGTSVSPEGPGLREAAHLPDPPRSENQQQGEGLNLSPSRPGTPKRWDERGSDSSFALLCQWIFGSTSTVTLPETLLFVSTLDGSLHAVSKRTGSIKWTLKEDPVLQVPTHVEEPAFLPDPNDGSLYTLGGKNNEGLTKLPFTIPELVQASPCRSSDGILYMGKKQDIWYVIDLLTGEKQQTLSSAFADSLCPSTSLLYLGRTEYTITMYDTKTRELRWNATYFDYAASLPEDDMDYKMSHFVSNGDGLVVTVDSESGDVLWIQNYASPVVAFYVWQREGLRKVMHINVAVETLRYLTFMSGEVGRITKWKYPFPKETEAKSKLMPTLYVGKYSTSLYASPSMVHEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITPSTDLKFDPGLKGKNKLHDLRNYWLLIGHHETPLSASTKMLERFPNNLPKHRENVIPADSEKNFEEVINLVDETSENAPTTVSQGVGEKFAHAPAKPEAPMDSMLKDVATIILSTFLLIGWVAFIITYPLSMHQQQQHQQFQKELEKIQLLQQQQLPFHPAGDATQDTELLDTSGPYSESSGTSSPSMSPRASNHSLQSASKAGTSLSLEQDDDDEETCMVIVGKISFCPKDVLGHGAEGTIVYRGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFCTERDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLQQTTSGLAHLHSLNIVHRDLKPHNILLSMPNAHGRIKAMISDFGLCKKLAVGRHSFSRRSGVPGTEGWIAPEMLSEDCKENPTYTVDIFSAGCVFYYVISEGNHPFGKSLQRQANILLGAYSLDCFHPEKHEDIIARELIEKMIAMDPQKRPSAKHVLKHPFFWSLEKQLQFFQDVSDRIEKESLDGPIVKQLERGGRAVVKMDWRENITVPLQTDLRKFRTYKGGSVRDLLRAMRNKRHHYRELPTEVRETLGALPDGFVRYFTSRFPHLLLHTYWAMELCGHERLFQPYYCHEPPEPWPTVTPDAL

Target ORF information:

RefSeq Version	XM_008587754.1
Organism	Galeopterus variegatus(Sunda flying lemur)
Definition	Galeopterus variegatus endoplasmic reticulum to nucleus signaling 1 (ERN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008587754.1

ORF Insert Sequence:

ATGAGGCACA ACCGGGGGTG TGAACTCTGC GACTGCCCTG CTCAGGGCAG AGACTCCGTG 
TTTGCTGGAA ACCCCTGCCA GCTGCTGAAA CCATCGCGCC AAAGGGCGTC TCAGTCAACC 
TCGCCCATGA CTGCCCCACG GCGGGAAGAC GAAAAGGGGG GTTTCTGGGC ACCCGGTACA 
GCCCGTGTCC CGGGTCCCTC GCCTGGGGGG TACGGGTTGT CGGTCTTGCC CAGCCTCGGA 
CGAGCTTCTT ACCTAGACCC TTCCCCGCTC CTGGACGCCG GACGCCGAGG CCTGGTCCTG 
CTCCTCAGCC GCCGCGTCCG CAGGACCCGA GCCAGTCAGA GGCCCCGCTC AGGCGTCCGC 
TGCCCCAGTG GCCTCCCGGG CCAGCGCGGC TGTCACGGGC TCGGGCGCAA CTCTGTGGCC 
CTGGGCGGCA CCTCGGTATC TCCAGAAGGC CCGGGCCTTC GGGAGGCCGC CCACCTGCCG 
GACCCTCCCC GCAGTGAGAA CCAACAGCAA GGCGAAGGGC TGAACCTCTC CCCCTCCAGG 
CCAGGTACAC CCAAGAGATG GGATGAGCGA GGTTCTGACA GCTCCTTCGC CCTCCTCTGC 
CAGTGGATTT TTGGAAGCAC CAGCACAGTG ACGCTTCCTG AAACCTTGCT GTTTGTGTCA 
ACCCTGGATG GCAGTTTGCA TGCTGTCAGC AAGAGGACAG GCTCAATCAA ATGGACATTA 
AAAGAAGATC CAGTCCTGCA GGTCCCAACA CATGTGGAAG AGCCTGCCTT CCTTCCAGAT 
CCCAATGATG GCAGCCTGTA TACGCTTGGA GGCAAGAATA ATGAAGGCCT GACAAAACTT 
CCCTTTACTA TCCCAGAGTT GGTACAGGCA TCCCCTTGCC GAAGTTCAGA TGGAATTCTG 
TACATGGGTA AAAAGCAGGA CATTTGGTAT GTTATCGACC TCCTGACCGG GGAGAAGCAG 
CAGACTTTGT CATCAGCCTT TGCAGACAGT CTCTGCCCAT CAACCTCTCT TCTGTATCTT 
GGGCGAACAG AATACACCAT CACTATGTAT GACACCAAAA CCCGAGAGCT CCGGTGGAAC 
GCCACCTACT TTGACTACGC AGCCTCACTG CCTGAGGACG ACATGGACTA CAAGATGTCC 
CACTTTGTGT CCAATGGTGA TGGGCTGGTG GTGACTGTGG ACAGTGAATC TGGGGACGTC 
CTGTGGATCC AAAACTATGC CTCCCCTGTG GTGGCCTTTT ATGTCTGGCA GCGGGAGGGT 
CTGAGGAAGG TGATGCACAT CAACGTTGCT GTGGAGACCC TGCGCTATCT GACCTTCATG 
TCTGGGGAGG TGGGACGCAT CACCAAGTGG AAGTACCCGT TCCCCAAGGA GACGGAGGCC 
AAGAGCAAGC TGATGCCCAC CCTGTATGTT GGGAAATACT CTACCAGCCT CTACGCCTCC 
CCCTCCATGG TACATGAAGG TGTCGCTGTC GTGCCCCGCG GCAGCACGCT TCCTTTGCTG 
GAAGGCCCCC AGACTGATGG TGTCACCATT GGGGACAAAG GAGAGTGTGT GATCACCCCC 
AGCACGGACC TCAAGTTCGA CCCTGGACTC AAGGGGAAGA ACAAGCTCCA TGACTTGAGG 
AACTACTGGC TTCTGATAGG ACACCATGAA ACCCCACTGT CTGCGTCTAC CAAGATGCTG 
GAGAGATTTC CCAACAATCT GCCGAAACAT CGGGAAAATG TGATTCCTGC TGATTCGGAG 
AAGAACTTTG AGGAGGTTAT CAACCTGGTT GACGAGACTT CGGAAAACGC GCCTACCACC 
GTGTCCCAGG GGGTGGGGGA GAAGTTTGCT CATGCACCTG CCAAGCCCGA GGCCCCCATG 
GACTCCATGC TCAAGGACGT GGCCACCATC ATCCTGAGCA CCTTCCTGCT CATCGGCTGG 
GTGGCCTTCA TCATCACCTA CCCCTTGAGC ATGCATCAGC AGCAGCAGCA CCAGCAGTTC 
CAGAAGGAAC TGGAGAAAAT CCAGCTCCTG CAACAGCAGC AGCTGCCCTT CCATCCAGCT 
GGAGATGCGA CTCAGGACAC CGAGCTCTTG GACACATCTG GGCCGTACTC GGAGAGCTCG 
GGCACCAGCA GCCCCAGCAT GTCCCCCCGA GCCTCCAACC ACTCACTCCA ATCTGCCTCT 
AAGGCTGGCA CAAGCCTGTC CCTGGAGCAG GATGACGACG ATGAGGAAAC CTGCATGGTG 
ATAGTTGGGA AAATTTCATT TTGTCCCAAA GACGTCCTGG GTCACGGAGC TGAGGGCACC 
ATTGTGTACC GGGGCATGTT TGACAACCGC GATGTGGCCG TGAAGAGGAT CCTCCCTGAG 
TGTTTTAGCT TTGCAGACCG TGAGGTCCAG CTGCTGCGAG AGTCAGACGA GCACCCAAAC 
GTGATCCGTT ACTTCTGCAC GGAGAGGGAC CGGCAGTTCC AGTACATTGC CATCGAGCTG 
TGCGCAGCCA CCTTGCAAGA GTACGTGGAG CAGAAGGACT TTGCCCACCT CGGCCTGGAG 
CCCATCACCT TGCTACAGCA GACCACCTCA GGCCTGGCCC ACCTGCACTC CCTCAACATT 
GTTCACAGAG ACCTGAAGCC CCACAACATT CTCCTCTCCA TGCCCAATGC CCATGGCAGG 
ATCAAGGCCA TGATCTCTGA CTTTGGCCTC TGCAAGAAGC TGGCAGTGGG CAGGCACAGC 
TTCAGCCGCC GTTCTGGGGT GCCCGGCACA GAAGGCTGGA TTGCTCCGGA GATGCTGAGT 
GAAGACTGCA AGGAGAACCC TACCTACACG GTGGACATCT TCTCCGCGGG CTGCGTCTTT 
TACTACGTGA TCTCTGAGGG CAACCACCCT TTCGGCAAGT CCCTGCAGCG GCAGGCTAAC 
ATCCTCCTGG GTGCCTACAG CCTCGACTGC TTCCACCCAG AGAAGCACGA AGACATCATT 
GCACGTGAAT TAATAGAGAA AATGATTGCC ATGGATCCTC AGAAACGCCC CTCAGCAAAG 
CACGTGCTAA AGCACCCGTT CTTCTGGAGC CTGGAGAAGC AGCTGCAGTT CTTTCAGGAT 
GTGAGTGACC GAATAGAAAA GGAGTCCCTG GATGGCCCAA TCGTGAAGCA GTTGGAGAGA 
GGCGGGAGAG CCGTGGTGAA GATGGACTGG CGGGAGAACA TCACAGTCCC CCTCCAGACA 
GATCTGCGTA AATTCAGAAC CTATAAAGGT GGCTCAGTCA GAGATCTCCT CCGAGCCATG 
AGAAACAAGA GGCACCACTA CCGGGAACTC CCCACGGAGG TGCGGGAGAC GCTGGGCGCG 
CTGCCCGACG GCTTCGTGCG CTACTTCACC TCACGCTTCC CCCACCTGCT CTTGCACACC 
TACTGGGCCA TGGAGCTGTG CGGCCACGAG AGACTCTTCC AGCCCTACTA CTGCCACGAG 
CCCCCGGAGC CCTGGCCCAC AGTGACTCCA GATGCCCTCT GA