ZAP70 cDNA ORF clone, Eurypyga helias(sunbittern)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZAP70
Entrez Gene ID	104514526
Full Name	zeta-chain (TCR) associated protein kinase 70kDa
Gene Type	protein-coding
Organism	Eurypyga helias(sunbittern)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010158244.1	XP_010156546.1	tyrosine-protein kinase ZAP-70

Pathways from BioSystems
Gene Ontology

The following ZAP70 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZAP70 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEu101389	XM_010158244.1 Latest version!	Eurypyga helias zeta-chain (TCR) associated protein kinase 70kDa (ZAP70), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZAP70 ( XM_010158244.1 ) cDNA ORF clone, Eurypyga helias(sunbittern) -> XP_010156546.1 Eurypyga helias zeta-chain (TCR) associated protein kinase 70kDa (ZAP70), mRNA.

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CloneID	OEu101389
Clone ID Related Accession (Same CDS sequence)	XM_010158244.1
Accession Version	XM_010158244.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-05
Organism	Eurypyga helias(sunbittern)
Product	tyrosine-protein kinase ZAP-70
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010269946.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Eurypyga helias Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGATG CTGCTGCTCA CCTGCCCTTC TACTACGGCA GCATTGCCAG GTCGGAGGCG 
GAGGAGTACC TCAAGCTGGC GGGGATGTCG GATGGCCTGT TCCTGCTGCG GCAATGCCTG 
CGCAGTCTGG GGGGGTATGT CCTCTCCATG GTCTGCAACC TGCAGTTTTA CCATTACGCC 
ATCGAGCGCC AGATGAACGG CACCTACGCC ATTGCGGGGG GCAAAGCGCA CTGTGGGCCG 
GAGGAGCTCT GTGAGTTTTA TTCCAAGGAT GCCGACGGGC TCTGCTGCAC CCTCCGCAAG 
CCTTGCAACC GCCCCAGTGG CCTGGAGCCC CAGCCCGGTA TCTTCGACAG CATGAGGGAC 
AATATGGTGC GCGAATATGT CCGGCAGACG TGGAAACTGG AGGGTGATGC ACTGGAACAG 
GCCATCATAA GCCAGGCTCC ACAGGTGGAG AAGCTCATTG CCACCACAGC CCATGAGAGG 
ATGGCCTGGT ACCATGGCAG CATCGTCCGG GATGAAGCTG AACGGAGGCT CTACTCTGGG 
GCACAGCCTG ATGGGAAATT CCTGCTGAGA GAACGGAAGG AAAATGGTAC CTATGCCCTC 
TCCCTTGTCT ATGGCAAAAC AGTTTATCAC TATCGGATAG ACCAGGACAA GTCTGGCAAG 
TACTCCATCC CCGAGGGGAC CAAGTTCGAC ACGCTCTGGC AGTTGGTGGA GTACCTGAAA 
CTCAAACCGG ATGGGCTGAT TTTCTACCTG AGGGAAACCT GCCCCAACCC CAACATGTCA 
GCATCTGCAG CACCGGTCCC ACCAACCCAC CCCTCCGTGA GCAGACACCT TGGGCCTCTC 
AATGCAGATG GATACACACC AGATCCTGTA GGAAAGTCAC GCCTGCTACC CATGGACACC 
AGTGTCTACG AGAGCCCATA CAGTGACCCT GAAGAACTAA AGGATAAGAA GCTTTTCCTG 
AAGAGGGACC ACCTGATGAT CGATGAAGTG GAACTGGGGG CAGGAAACTT TGGCTGCGTC 
AAGAAAGGAG TCTACAAAAT GAGAAAGAAG CAGATAGATG TGGCCATTAA GGTGCTGAAG 
AGCAACAATG AGAAAACAGT GAAGGATGAA ATGATGAAGG AAGCCCAGAT CATGCATCAG 
CTGGACAACC CCTACATTGT CCGCATGATT GGGGTCTGCG AAGCAGAGTC GCTGATGCTC 
GTGATGGAGA TGGCTTCTGG AGGGCCACTC AACAAGTTCT TGGCTTCCAA GAAAGATGAG 
ATTACCGTCA GCAATATTGT GGAGCTAATG CACCAAGTAT CCATGGGGAT GAAGTACTTG 
GAGGAAAAAA ACTTTGTCCA CAGGGACTTG GCAGCCAGAA ACGTCCTGCT GGTCAATCAG 
CATTATGCCA AGATCAGTGA CTTTGGACTC TCCAAGGCCC TTGGCGCTGA TGACAGCTAC 
TACAAGGCCA GAACAGCAGG AAAGTGGCCC TTGAAATGGT ATGCCCCAGA GTGCATCCTC 
TATCACAAGT TTTCCAGCAA GAGTGATGTA TGGAGCTACG GTGTGACCAT GTGGGAGGCC 
TTCAGCTTCG GGCAGAAACC GTACAAGAAA ATGAAAGGCC CAGAGGTCAT CAGCTTCATA 
GAGCAAGGGA AGCGCATGGA CTGCCCCACA GACTGCCCGG CAGAGATGTA TGCCCTCATG 
CAGCAATGCT GGACCTACAG GTATTCTGTG CGTCCAGGAT TTATTTCTGT GGAAAACATA 
ATCCGCTCCT ACTACTACAG TATTGCTACC AAGACAGAAA ATGGGCCAGA GACAGAGGAC 
AAGTCAAAAG CAACTCTTCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010156546.1
CDS	1..1824
Translation	MPDAAAHLPFYYGSIARSEAEEYLKLAGMSDGLFLLRQCLRSLGGYVLSMVCNLQFYHYAIERQMNGTYAIAGGKAHCGPEELCEFYSKDADGLCCTLRKPCNRPSGLEPQPGIFDSMRDNMVREYVRQTWKLEGDALEQAIISQAPQVEKLIATTAHERMAWYHGSIVRDEAERRLYSGAQPDGKFLLRERKENGTYALSLVYGKTVYHYRIDQDKSGKYSIPEGTKFDTLWQLVEYLKLKPDGLIFYLRETCPNPNMSASAAPVPPTHPSVSRHLGPLNADGYTPDPVGKSRLLPMDTSVYESPYSDPEELKDKKLFLKRDHLMIDEVELGAGNFGCVKKGVYKMRKKQIDVAIKVLKSNNEKTVKDEMMKEAQIMHQLDNPYIVRMIGVCEAESLMLVMEMASGGPLNKFLASKKDEITVSNIVELMHQVSMGMKYLEEKNFVHRDLAARNVLLVNQHYAKISDFGLSKALGADDSYYKARTAGKWPLKWYAPECILYHKFSSKSDVWSYGVTMWEAFSFGQKPYKKMKGPEVISFIEQGKRMDCPTDCPAEMYALMQQCWTYRYSVRPGFISVENIIRSYYYSIATKTENGPETEDKSKATLP

Target ORF information:

RefSeq Version	XM_010158244.1
Organism	Eurypyga helias(sunbittern)
Definition	Eurypyga helias zeta-chain (TCR) associated protein kinase 70kDa (ZAP70), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010158244.1

ORF Insert Sequence:

ATGCCAGATG CTGCTGCTCA CCTGCCCTTC TACTACGGCA GCATTGCCAG GTCGGAGGCG 
GAGGAGTACC TCAAGCTGGC GGGGATGTCG GATGGCCTGT TCCTGCTGCG GCAATGCCTG 
CGCAGTCTGG GGGGGTATGT CCTCTCCATG GTCTGCAACC TGCAGTTTTA CCATTACGCC 
ATCGAGCGCC AGATGAACGG CACCTACGCC ATTGCGGGGG GCAAAGCGCA CTGTGGGCCG 
GAGGAGCTCT GTGAGTTTTA TTCCAAGGAT GCCGACGGGC TCTGCTGCAC CCTCCGCAAG 
CCTTGCAACC GCCCCAGTGG CCTGGAGCCC CAGCCCGGTA TCTTCGACAG CATGAGGGAC 
AATATGGTGC GCGAATATGT CCGGCAGACG TGGAAACTGG AGGGTGATGC ACTGGAACAG 
GCCATCATAA GCCAGGCTCC ACAGGTGGAG AAGCTCATTG CCACCACAGC CCATGAGAGG 
ATGGCCTGGT ACCATGGCAG CATCGTCCGG GATGAAGCTG AACGGAGGCT CTACTCTGGG 
GCACAGCCTG ATGGGAAATT CCTGCTGAGA GAACGGAAGG AAAATGGTAC CTATGCCCTC 
TCCCTTGTCT ATGGCAAAAC AGTTTATCAC TATCGGATAG ACCAGGACAA GTCTGGCAAG 
TACTCCATCC CCGAGGGGAC CAAGTTCGAC ACGCTCTGGC AGTTGGTGGA GTACCTGAAA 
CTCAAACCGG ATGGGCTGAT TTTCTACCTG AGGGAAACCT GCCCCAACCC CAACATGTCA 
GCATCTGCAG CACCGGTCCC ACCAACCCAC CCCTCCGTGA GCAGACACCT TGGGCCTCTC 
AATGCAGATG GATACACACC AGATCCTGTA GGAAAGTCAC GCCTGCTACC CATGGACACC 
AGTGTCTACG AGAGCCCATA CAGTGACCCT GAAGAACTAA AGGATAAGAA GCTTTTCCTG 
AAGAGGGACC ACCTGATGAT CGATGAAGTG GAACTGGGGG CAGGAAACTT TGGCTGCGTC 
AAGAAAGGAG TCTACAAAAT GAGAAAGAAG CAGATAGATG TGGCCATTAA GGTGCTGAAG 
AGCAACAATG AGAAAACAGT GAAGGATGAA ATGATGAAGG AAGCCCAGAT CATGCATCAG 
CTGGACAACC CCTACATTGT CCGCATGATT GGGGTCTGCG AAGCAGAGTC GCTGATGCTC 
GTGATGGAGA TGGCTTCTGG AGGGCCACTC AACAAGTTCT TGGCTTCCAA GAAAGATGAG 
ATTACCGTCA GCAATATTGT GGAGCTAATG CACCAAGTAT CCATGGGGAT GAAGTACTTG 
GAGGAAAAAA ACTTTGTCCA CAGGGACTTG GCAGCCAGAA ACGTCCTGCT GGTCAATCAG 
CATTATGCCA AGATCAGTGA CTTTGGACTC TCCAAGGCCC TTGGCGCTGA TGACAGCTAC 
TACAAGGCCA GAACAGCAGG AAAGTGGCCC TTGAAATGGT ATGCCCCAGA GTGCATCCTC 
TATCACAAGT TTTCCAGCAA GAGTGATGTA TGGAGCTACG GTGTGACCAT GTGGGAGGCC 
TTCAGCTTCG GGCAGAAACC GTACAAGAAA ATGAAAGGCC CAGAGGTCAT CAGCTTCATA 
GAGCAAGGGA AGCGCATGGA CTGCCCCACA GACTGCCCGG CAGAGATGTA TGCCCTCATG 
CAGCAATGCT GGACCTACAG GTATTCTGTG CGTCCAGGAT TTATTTCTGT GGAAAACATA 
ATCCGCTCCT ACTACTACAG TATTGCTACC AAGACAGAAA ATGGGCCAGA GACAGAGGAC 
AAGTCAAAAG CAACTCTTCC TTGA