LOC108954266 cDNA ORF clone, Eucalyptus grandis

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108954266
Entrez Gene ID	108954266
Full Name	uncharacterized LOC108954266
Gene Type	protein-coding
Organism	Eucalyptus grandis

mRNA and Protein(s)

mRNA	Protein	Name
XM_018860031.1	XP_018715576.1	uncharacterized protein LOC108954266
XM_018860030.1	XP_018715575.1	uncharacterized protein LOC108954266

Pathways from BioSystems
Gene Ontology

The following LOC108954266 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108954266 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEu39926	XM_018860030.1 Latest version!	Eucalyptus grandis uncharacterized LOC108954266 (LOC108954266), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OEu39926	XM_018860031.1 Latest version!	Eucalyptus grandis uncharacterized LOC108954266 (LOC108954266), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108954266 ( XM_018860031.1 ) cDNA ORF clone, Eucalyptus grandis -> XP_018715576.1 Eucalyptus grandis uncharacterized LOC108954266 (LOC108954266), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OEu39926
Clone ID Related Accession (Same CDS sequence)	XM_018860031.1 , XM_018860030.1
Accession Version	XM_018860031.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-24
Organism	Eucalyptus grandis
Product	uncharacterized protein LOC108954266
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010092445.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Eucalyptus grandis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATATAT TTGATGGCAA GAAACTAGCA ATCCTTGCTT CTGGCATTTA CAAGTTGCAA 
AATCTTGAAA GCCTACAGCT TGAAGGCTAC CCAACACTTA CCACGTGTCT AAAGGAGGAA 
GAGGATTTGA GGGAACCCCA TAAAAAGATG GGATTTCCTA TGTTATCGGG AAAAATTTTT 
ACTAAAATTC CTACGTGGGA GAAATCATAT CACTTGGAAT CTGAGGATCT TTCGCATAGT 
CGAGATCCAT TTCCCTCCAA CATATGTGAC ATTGAGGATG TTGAGTTATA TTCAAGTGGA 
GAGCCAGTCC ATAATGGGTT CCCCAAGCTT GAGAAATTTC ATCGCAAGAC AAATTGCCGA 
ATTATTCTAC CTGGAGGAGA GATGCCAAAA TGGTTGCTTC CCAATAGAGA GGGTTATATA 
TCTTTCGTGG CTTCAAAGGA CTTATATAAA AAGATTTTAG GAGTAGCTTT CTGTGTTGTA 
TTTCAATTAA AAGCAATGAC GGCTGCCAGT GGTGCGCTTT ACCCATATGA GCTTATAGGA 
TATGTTAATT GCAAAGGCAT TAAACACCCC AAGTTTGTAC GGCCATTTGG TTTGGATCAT 
GTGTGGTGTG AGCATCTAAG ATCAAAGGAT CTCAATTTGG ATCATGTGTG GCTTGAGTAT 
ATGGAATCAA AGGATTTGTG GCCAGTAGAC CATTTTGGTC CAAATGACTC GGGTGATTTT 
TACTTTGGCA TTAGAGCATG CGGTTTTTAT ATTGATACAA GAGATGGTGG ACTTGTAAAA 
AATTGTGGAT TCCGACTAAT ATGCAAGCCA TTAGAGAAAG ATTTGAAGGT TTTGCTTCAA 
GACAATAAGT TGCTAGATCC AGCTTTACTC TACGAGGTTT CACATGGAGA TAATCTAATG 
AGCACAAAGG AAGAAAGTTC AAGTAAAACG GAAGGTTGTG GAGTGTGCAA AAAGGTCAAA 
GGATTGAACA TTGTTGACAT CTCAATTGAG AGGCATCGCT ATTCTAGCGT TTGGTCTACG 
TTACGAAATG TTAGCCCCGG GAGAGAGATG CCAAAGGGGT TCGTCCTTAT TGAAGACGGT 
ACTATATCTT TCAAGGCTTC GCAAGACTTG TACAACAAGT TCAAAGGATT GTATCTCTGT 
GTTGTCTTTG GTGTAGAAGA TGGAGAAAAG GAAGTATCTT TCGACATCGT ACCACGTGTT 
AACGGCCAAA GGCGGAATGA GCTATCAGGA ACTCTCGGTT CATTTGATAT GGATCACGTG 
TGGTTTCAAC TTTTCACACC AAATGCACTA TGGGGATTAT TGGAGGGAGA AGTTGAATTT 
GGTCAATTTG AAAAGAGGTA TCTACGATTT AGCCTTAATA TTAGACTAGC GGGTGCAACT 
GTGAAAAAGA TGGGATACGT GATGCAATCC AATCCACTGG AGGATGCTTT GGAGGTTGAG 
CTTGAAAAGA ATCGATTAAT GGATCCAGCC TCACTCCTTT TGCATTTTGA GAATCCACTG 
TACTATGGGA GCCACGGATG GAAGCGTTTG TTCAAGATAC TCGAAGATGT ACATGGACGG 
AGAGACTCAC TCGAAGATAT GCATGGAGGG AGAGACTCGA AGATATGCAT GGTGGCACAG 
ACTGGACATA TTATAAGAGG GGCATCATGC GTGATTCATG TGCCATCACG CGAGAGCTGC 
GAGGGCCGCG AGAGGTGCGA GATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018715576.1
CDS	419..2125
Translation	MYIFDGKKLAILASGIYKLQNLESLQLEGYPTLTTCLKEEEDLREPHKKMGFPMLSGKIFTKIPTWEKSYHLESEDLSHSRDPFPSNICDIEDVELYSSGEPVHNGFPKLEKFHRKTNCRIILPGGEMPKWLLPNREGYISFVASKDLYKKILGVAFCVVFQLKAMTAASGALYPYELIGYVNCKGIKHPKFVRPFGLDHVWCEHLRSKDLNLDHVWLEYMESKDLWPVDHFGPNDSGDFYFGIRACGFYIDTRDGGLVKNCGFRLICKPLEKDLKVLLQDNKLLDPALLYEVSHGDNLMSTKEESSSKTEGCGVCKKVKGLNIVDISIERHRYSSVWSTLRNVSPGREMPKGFVLIEDGTISFKASQDLYNKFKGLYLCVVFGVEDGEKEVSFDIVPRVNGQRRNELSGTLGSFDMDHVWFQLFTPNALWGLLEGEVEFGQFEKRYLRFSLNIRLAGATVKKMGYVMQSNPLEDALEVELEKNRLMDPASLLLHFENPLYYGSHGWKRLFKILEDVHGRRDSLEDMHGGRDSKICMVAQTGHIIRGASCVIHVPSRESCEGRERCEI

Target ORF information:

RefSeq Version	XM_018860031.1
Organism	Eucalyptus grandis
Definition	Eucalyptus grandis uncharacterized LOC108954266 (LOC108954266), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018860031.1

ORF Insert Sequence:

ATGTATATAT TTGATGGCAA GAAACTAGCA ATCCTTGCTT CTGGCATTTA CAAGTTGCAA 
AATCTTGAAA GCCTACAGCT TGAAGGCTAC CCAACACTTA CCACGTGTCT AAAGGAGGAA 
GAGGATTTGA GGGAACCCCA TAAAAAGATG GGATTTCCTA TGTTATCGGG AAAAATTTTT 
ACTAAAATTC CTACGTGGGA GAAATCATAT CACTTGGAAT CTGAGGATCT TTCGCATAGT 
CGAGATCCAT TTCCCTCCAA CATATGTGAC ATTGAGGATG TTGAGTTATA TTCAAGTGGA 
GAGCCAGTCC ATAATGGGTT CCCCAAGCTT GAGAAATTTC ATCGCAAGAC AAATTGCCGA 
ATTATTCTAC CTGGAGGAGA GATGCCAAAA TGGTTGCTTC CCAATAGAGA GGGTTATATA 
TCTTTCGTGG CTTCAAAGGA CTTATATAAA AAGATTTTAG GAGTAGCTTT CTGTGTTGTA 
TTTCAATTAA AAGCAATGAC GGCTGCCAGT GGTGCGCTTT ACCCATATGA GCTTATAGGA 
TATGTTAATT GCAAAGGCAT TAAACACCCC AAGTTTGTAC GGCCATTTGG TTTGGATCAT 
GTGTGGTGTG AGCATCTAAG ATCAAAGGAT CTCAATTTGG ATCATGTGTG GCTTGAGTAT 
ATGGAATCAA AGGATTTGTG GCCAGTAGAC CATTTTGGTC CAAATGACTC GGGTGATTTT 
TACTTTGGCA TTAGAGCATG CGGTTTTTAT ATTGATACAA GAGATGGTGG ACTTGTAAAA 
AATTGTGGAT TCCGACTAAT ATGCAAGCCA TTAGAGAAAG ATTTGAAGGT TTTGCTTCAA 
GACAATAAGT TGCTAGATCC AGCTTTACTC TACGAGGTTT CACATGGAGA TAATCTAATG 
AGCACAAAGG AAGAAAGTTC AAGTAAAACG GAAGGTTGTG GAGTGTGCAA AAAGGTCAAA 
GGATTGAACA TTGTTGACAT CTCAATTGAG AGGCATCGCT ATTCTAGCGT TTGGTCTACG 
TTACGAAATG TTAGCCCCGG GAGAGAGATG CCAAAGGGGT TCGTCCTTAT TGAAGACGGT 
ACTATATCTT TCAAGGCTTC GCAAGACTTG TACAACAAGT TCAAAGGATT GTATCTCTGT 
GTTGTCTTTG GTGTAGAAGA TGGAGAAAAG GAAGTATCTT TCGACATCGT ACCACGTGTT 
AACGGCCAAA GGCGGAATGA GCTATCAGGA ACTCTCGGTT CATTTGATAT GGATCACGTG 
TGGTTTCAAC TTTTCACACC AAATGCACTA TGGGGATTAT TGGAGGGAGA AGTTGAATTT 
GGTCAATTTG AAAAGAGGTA TCTACGATTT AGCCTTAATA TTAGACTAGC GGGTGCAACT 
GTGAAAAAGA TGGGATACGT GATGCAATCC AATCCACTGG AGGATGCTTT GGAGGTTGAG 
CTTGAAAAGA ATCGATTAAT GGATCCAGCC TCACTCCTTT TGCATTTTGA GAATCCACTG 
TACTATGGGA GCCACGGATG GAAGCGTTTG TTCAAGATAC TCGAAGATGT ACATGGACGG 
AGAGACTCAC TCGAAGATAT GCATGGAGGG AGAGACTCGA AGATATGCAT GGTGGCACAG 
ACTGGACATA TTATAAGAGG GGCATCATGC GTGATTCATG TGCCATCACG CGAGAGCTGC 
GAGGGCCGCG AGAGGTGCGA GATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108954266 ( XM_018860030.1 ) cDNA ORF clone, Eucalyptus grandis -> XP_018715575.1 Eucalyptus grandis uncharacterized LOC108954266 (LOC108954266), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OEu39926
Clone ID Related Accession (Same CDS sequence)	XM_018860031.1 , XM_018860030.1
Accession Version	XM_018860030.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-24
Organism	Eucalyptus grandis
Product	uncharacterized protein LOC108954266
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010092445.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Eucalyptus grandis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATATAT TTGATGGCAA GAAACTAGCA ATCCTTGCTT CTGGCATTTA CAAGTTGCAA 
AATCTTGAAA GCCTACAGCT TGAAGGCTAC CCAACACTTA CCACGTGTCT AAAGGAGGAA 
GAGGATTTGA GGGAACCCCA TAAAAAGATG GGATTTCCTA TGTTATCGGG AAAAATTTTT 
ACTAAAATTC CTACGTGGGA GAAATCATAT CACTTGGAAT CTGAGGATCT TTCGCATAGT 
CGAGATCCAT TTCCCTCCAA CATATGTGAC ATTGAGGATG TTGAGTTATA TTCAAGTGGA 
GAGCCAGTCC ATAATGGGTT CCCCAAGCTT GAGAAATTTC ATCGCAAGAC AAATTGCCGA 
ATTATTCTAC CTGGAGGAGA GATGCCAAAA TGGTTGCTTC CCAATAGAGA GGGTTATATA 
TCTTTCGTGG CTTCAAAGGA CTTATATAAA AAGATTTTAG GAGTAGCTTT CTGTGTTGTA 
TTTCAATTAA AAGCAATGAC GGCTGCCAGT GGTGCGCTTT ACCCATATGA GCTTATAGGA 
TATGTTAATT GCAAAGGCAT TAAACACCCC AAGTTTGTAC GGCCATTTGG TTTGGATCAT 
GTGTGGTGTG AGCATCTAAG ATCAAAGGAT CTCAATTTGG ATCATGTGTG GCTTGAGTAT 
ATGGAATCAA AGGATTTGTG GCCAGTAGAC CATTTTGGTC CAAATGACTC GGGTGATTTT 
TACTTTGGCA TTAGAGCATG CGGTTTTTAT ATTGATACAA GAGATGGTGG ACTTGTAAAA 
AATTGTGGAT TCCGACTAAT ATGCAAGCCA TTAGAGAAAG ATTTGAAGGT TTTGCTTCAA 
GACAATAAGT TGCTAGATCC AGCTTTACTC TACGAGGTTT CACATGGAGA TAATCTAATG 
AGCACAAAGG AAGAAAGTTC AAGTAAAACG GAAGGTTGTG GAGTGTGCAA AAAGGTCAAA 
GGATTGAACA TTGTTGACAT CTCAATTGAG AGGCATCGCT ATTCTAGCGT TTGGTCTACG 
TTACGAAATG TTAGCCCCGG GAGAGAGATG CCAAAGGGGT TCGTCCTTAT TGAAGACGGT 
ACTATATCTT TCAAGGCTTC GCAAGACTTG TACAACAAGT TCAAAGGATT GTATCTCTGT 
GTTGTCTTTG GTGTAGAAGA TGGAGAAAAG GAAGTATCTT TCGACATCGT ACCACGTGTT 
AACGGCCAAA GGCGGAATGA GCTATCAGGA ACTCTCGGTT CATTTGATAT GGATCACGTG 
TGGTTTCAAC TTTTCACACC AAATGCACTA TGGGGATTAT TGGAGGGAGA AGTTGAATTT 
GGTCAATTTG AAAAGAGGTA TCTACGATTT AGCCTTAATA TTAGACTAGC GGGTGCAACT 
GTGAAAAAGA TGGGATACGT GATGCAATCC AATCCACTGG AGGATGCTTT GGAGGTTGAG 
CTTGAAAAGA ATCGATTAAT GGATCCAGCC TCACTCCTTT TGCATTTTGA GAATCCACTG 
TACTATGGGA GCCACGGATG GAAGCGTTTG TTCAAGATAC TCGAAGATGT ACATGGACGG 
AGAGACTCAC TCGAAGATAT GCATGGAGGG AGAGACTCGA AGATATGCAT GGTGGCACAG 
ACTGGACATA TTATAAGAGG GGCATCATGC GTGATTCATG TGCCATCACG CGAGAGCTGC 
GAGGGCCGCG AGAGGTGCGA GATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018715575.1
CDS	1743..3449
Translation	MYIFDGKKLAILASGIYKLQNLESLQLEGYPTLTTCLKEEEDLREPHKKMGFPMLSGKIFTKIPTWEKSYHLESEDLSHSRDPFPSNICDIEDVELYSSGEPVHNGFPKLEKFHRKTNCRIILPGGEMPKWLLPNREGYISFVASKDLYKKILGVAFCVVFQLKAMTAASGALYPYELIGYVNCKGIKHPKFVRPFGLDHVWCEHLRSKDLNLDHVWLEYMESKDLWPVDHFGPNDSGDFYFGIRACGFYIDTRDGGLVKNCGFRLICKPLEKDLKVLLQDNKLLDPALLYEVSHGDNLMSTKEESSSKTEGCGVCKKVKGLNIVDISIERHRYSSVWSTLRNVSPGREMPKGFVLIEDGTISFKASQDLYNKFKGLYLCVVFGVEDGEKEVSFDIVPRVNGQRRNELSGTLGSFDMDHVWFQLFTPNALWGLLEGEVEFGQFEKRYLRFSLNIRLAGATVKKMGYVMQSNPLEDALEVELEKNRLMDPASLLLHFENPLYYGSHGWKRLFKILEDVHGRRDSLEDMHGGRDSKICMVAQTGHIIRGASCVIHVPSRESCEGRERCEI

Target ORF information:

RefSeq Version	XM_018860030.1
Organism	Eucalyptus grandis
Definition	Eucalyptus grandis uncharacterized LOC108954266 (LOC108954266), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018860030.1

ORF Insert Sequence:

ATGTATATAT TTGATGGCAA GAAACTAGCA ATCCTTGCTT CTGGCATTTA CAAGTTGCAA 
AATCTTGAAA GCCTACAGCT TGAAGGCTAC CCAACACTTA CCACGTGTCT AAAGGAGGAA 
GAGGATTTGA GGGAACCCCA TAAAAAGATG GGATTTCCTA TGTTATCGGG AAAAATTTTT 
ACTAAAATTC CTACGTGGGA GAAATCATAT CACTTGGAAT CTGAGGATCT TTCGCATAGT 
CGAGATCCAT TTCCCTCCAA CATATGTGAC ATTGAGGATG TTGAGTTATA TTCAAGTGGA 
GAGCCAGTCC ATAATGGGTT CCCCAAGCTT GAGAAATTTC ATCGCAAGAC AAATTGCCGA 
ATTATTCTAC CTGGAGGAGA GATGCCAAAA TGGTTGCTTC CCAATAGAGA GGGTTATATA 
TCTTTCGTGG CTTCAAAGGA CTTATATAAA AAGATTTTAG GAGTAGCTTT CTGTGTTGTA 
TTTCAATTAA AAGCAATGAC GGCTGCCAGT GGTGCGCTTT ACCCATATGA GCTTATAGGA 
TATGTTAATT GCAAAGGCAT TAAACACCCC AAGTTTGTAC GGCCATTTGG TTTGGATCAT 
GTGTGGTGTG AGCATCTAAG ATCAAAGGAT CTCAATTTGG ATCATGTGTG GCTTGAGTAT 
ATGGAATCAA AGGATTTGTG GCCAGTAGAC CATTTTGGTC CAAATGACTC GGGTGATTTT 
TACTTTGGCA TTAGAGCATG CGGTTTTTAT ATTGATACAA GAGATGGTGG ACTTGTAAAA 
AATTGTGGAT TCCGACTAAT ATGCAAGCCA TTAGAGAAAG ATTTGAAGGT TTTGCTTCAA 
GACAATAAGT TGCTAGATCC AGCTTTACTC TACGAGGTTT CACATGGAGA TAATCTAATG 
AGCACAAAGG AAGAAAGTTC AAGTAAAACG GAAGGTTGTG GAGTGTGCAA AAAGGTCAAA 
GGATTGAACA TTGTTGACAT CTCAATTGAG AGGCATCGCT ATTCTAGCGT TTGGTCTACG 
TTACGAAATG TTAGCCCCGG GAGAGAGATG CCAAAGGGGT TCGTCCTTAT TGAAGACGGT 
ACTATATCTT TCAAGGCTTC GCAAGACTTG TACAACAAGT TCAAAGGATT GTATCTCTGT 
GTTGTCTTTG GTGTAGAAGA TGGAGAAAAG GAAGTATCTT TCGACATCGT ACCACGTGTT 
AACGGCCAAA GGCGGAATGA GCTATCAGGA ACTCTCGGTT CATTTGATAT GGATCACGTG 
TGGTTTCAAC TTTTCACACC AAATGCACTA TGGGGATTAT TGGAGGGAGA AGTTGAATTT 
GGTCAATTTG AAAAGAGGTA TCTACGATTT AGCCTTAATA TTAGACTAGC GGGTGCAACT 
GTGAAAAAGA TGGGATACGT GATGCAATCC AATCCACTGG AGGATGCTTT GGAGGTTGAG 
CTTGAAAAGA ATCGATTAAT GGATCCAGCC TCACTCCTTT TGCATTTTGA GAATCCACTG 
TACTATGGGA GCCACGGATG GAAGCGTTTG TTCAAGATAC TCGAAGATGT ACATGGACGG 
AGAGACTCAC TCGAAGATAT GCATGGAGGG AGAGACTCGA AGATATGCAT GGTGGCACAG 
ACTGGACATA TTATAAGAGG GGCATCATGC GTGATTCATG TGCCATCACG CGAGAGCTGC 
GAGGGCCGCG AGAGGTGCGA GATCTAA