VPS50 cDNA ORF clone, Erinaceus europaeus(western European hedgehog)

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS50
Entrez Gene ID	103113035
Full Name	VPS50, EARP/GARPII complex subunit
Synonyms	CCDC132
Gene Type	protein-coding
Organism	Erinaceus europaeus(western European hedgehog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007522514.2	XP_007522576.1	syndetin

Pathways from BioSystems
Gene Ontology

The following VPS50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEr10352	XM_007522514.2 Latest version!	Erinaceus europaeus VPS50, EARP/GARPII complex subunit (VPS50), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS50 ( XM_007522514.2 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_007522576.1 Erinaceus europaeus VPS50, EARP/GARPII complex subunit (VPS50), mRNA.

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CloneID	OEr10352
Clone ID Related Accession (Same CDS sequence)	XM_007522514.2
Accession Version	XM_007522514.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	syndetin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006804103.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Apr 11, 2016 this sequence version replaced XM_007522514.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGCAAAAAA TCAAATCTCT TATGACCCGA CAGGGTCTGA GAAGTCCTCC AGAGAACCTC 
AGTGATCTTG GTGCCATTGA GAGTCTCCGG GTCCCTGGAA AGGAGGAATT TCGAGAACTT 
CGAGAACAAC CCAGTGATCC TCAGGCTGAA CAAGAACTAA TTAATAGTAT TGAACAAGTA 
TATTTTTCTA CAGACTCCTT TGATATTGTT AAATATGAGC TGGAGAAACT CCCTCCTATT 
CTCAGTTTGC AAGAACTAGA GGAGTATAGA GACAAATTAA AACAACAGCA AGCTGCGGTC 
TCTAAAAAAG TGGCAGATTT AATTCTTGAA AAACAGCCTG CTTATGTAAA GGTGGGGCAT 
CTAAATATTG CAAAGGAAGG TTTTACTCAA GCTAGTTTAG GCCTTCTTGC AAATCAAAGG 
AAACGCCAGT TGCTGATTGG ACTTCTGAAG TCTCTGAGAA CTATAAAAAC ATTGCAAAGA 
ACAGATGTAC GCTTAAGTGA AATGCTAGAG GAAGAAGACT ACCCAGGAGC TATTCAGCTA 
TGTCTGGAAT GTCAGAAAGC TGCCAGCACT TTCAAACATT ATAGTTGCAT AAGTGAACTA 
AATTCAAAGC TGCAAGATAC TTTAGAACAA ATTGAGGAAC AGTTGGATGT AGCACTTTCC 
AAAATCTGTA AGAATTTTGA CATTAACCAT TACACTAAGG TTCAACAAGC TTATCGACTT 
CTTGGAAAAA CACAGACAGC CATGGATCAA CTTCATATGC ACTTTACCCA GGCCATTCAC 
AATACTGTGT TCCAAGTTGT TCTTGGCTAT GTGGAGTTAT GTGCAGGAAA CACGGATACA 
AAATTCCAAA AACTTCAATA TAAGGATCTC TGTACTCATG TTACACCAGA CAGCTATATT 
CCATGCCTTG CTGACCTGTG CAAAGCACTG TGGGAAGTTA TGCTCAGTTA TTACAGAACT 
ATGGAATGGC ATGAAAAACA TGATAATGAA GATACTACAT CTACTTCTGA AGGGAGTAAT 
ATGATTGGTA CCGAAGAAAC TAATTTTGAT CGTGGCTATA TAAAAAAGAA GTTAGAACAT 
GGACTTACAC GAATATGGCA GGATGTTCAG CTAAAAGTGA AAACCTATTT ACTTGGAACT 
GATTTATCAA TATTCAAATA TGATGACTTC ATCTTTGTTT TGGATATAGT CAGCAGGTTG 
ATGCAAGTTG GAGAAGAATT TTGTGGTAGT AAATCTGAAG TTTTGCAGGA GTCTATTAGG 
AAACAAAGTG TCAATTATTT TAAGAACTAC CATAGAACAC GGCTTGATGA ACTGAGAATG 
TTCCTGGAAA ATGAGACTTG GGAACTTTGT CCCGTTAAGT CTAATTTCAG CATCTTGCAA 
CTTCATGAAT TTAAATTTAT GGAACAGTCT CGTTCCCCAT CTGTTTCACC TAGTAAGCAG 
CCAGCCTCAA CTTCCTCAAA AACAGTTAAC TTGTTTGAGC AGTACTGCAG TGGTGGGAAT 
CCATTTGAAA TTCAGGCCAA CCACAAGGAT GAAGAAACAG AAGATGTTCT GGCTTCTAAT 
GGGTATGAAT CTGATGAACA AGAAAAAAGT GCCTATCAGG AATATGACAG TGACAGTGAT 
GTTCCTGAGG AACTAAAACG GGATTATGTG GATGAACAGA CTGGTGATGC TCCTGTAAAA 
AGTGTTTCTC GAGAAACTCT AAAAATCAGG AAGAAATCCG ATTACAGTCT AAATAAAGTG 
AATGCGCCCA TCTTAACAAA TACAACACTG AATGTAATAA GACTTGTTGG TAAATATATG 
CAGATGATGA ACATTCTTAA GCCAATTGCC TTTGATGTTA TACACTTCAT GTCTCAGCTA 
TTTGACTATT ACTTGTATGC AATCTATACC TTTTTTGGTC GGAATGATTC ATTAGAATCA 
ACAGGACTAG GTCTAAGTAG TAGTAGACTA AGAACAACTC TAAACAGAAT ACAGGAAAGT 
CTTATTGATC TGGAAGTTTC ATCTGACCCT ACTGCCACAC TGACAGCAGC AGAAGAGAGA 
AAGGAGAAGG TTCCAAGCCC ACACCTTAGT CACCTAGTGG TTTTGACATC TGGAGATACA 
TTGTATGGGT TGGCAGAAAG AGTGGTAGCC ACAGAATCCT TGGTATTCTT GGCTGAACAA 
TTTGAGTTCC TTCAGCCACA TCTGGATGCC GTGATGCCTG CGGTCAAAAA GCCCTTTCTT 
CAGCAGTTTT ATTCTCAGAC AGTCTCAACT GCCAGTGAAC TACGCAAACC AATTTACTGG 
ATTGTAGCTG GTAAAGCCAT TGATTATGAA CAAATGCTAC TTCTAATGAC TAATGTGAAA 
TGGGATGTCA AAGAAATTAT GTCACAGCAC AACATATATG TAGATGCATT GCTAAAGGAA 
TTTGAGCAGT TTAACAGGAG GTTAAACGAG GTTTCTAAGA GAGTACGGAT ACCCTTGCCT 
GTGTCTAATA TACTTTGGGA ACATTGTATA CGACTGGCTA ATCGAACTAT TGTGGAAGGA 
TATGCTAATG TCAAGAAGTG TAGCAATGAG GGTCGTGCCT TGATGCAATT AGATTTTCAA 
CAGTTTTTAA TGAAACTGGA AAAACTAACA GATATTAGAC CCATTCCTGA TAAAGAATTT 
GTGGAAACCT ATATTAAAGC CTACTATCTA ACTGAGAATG ACATGGAACG CTGGATCAAA 
GAGCACAGGG AGTATTCAAC CAAGCAGCTG ACCAATCTGG TGAATGTTTG CCTGGGATCC 
CATATCAACA AGAAAGCAAG ACAAAAACTT CTATCAGCCA TAGATGATAT AGACAGGCCC 
AAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007522576.1
CDS	87..2915
Translation	MQKIKSLMTRQGLRSPPENLSDLGAIESLRVPGKEEFRELREQPSDPQAEQELINSIEQVYFSTDSFDIVKYELEKLPPILSLQELEEYRDKLKQQQAAVSKKVADLILEKQPAYVKVGHLNIAKEGFTQASLGLLANQRKRQLLIGLLKSLRTIKTLQRTDVRLSEMLEEEDYPGAIQLCLECQKAASTFKHYSCISELNSKLQDTLEQIEEQLDVALSKICKNFDINHYTKVQQAYRLLGKTQTAMDQLHMHFTQAIHNTVFQVVLGYVELCAGNTDTKFQKLQYKDLCTHVTPDSYIPCLADLCKALWEVMLSYYRTMEWHEKHDNEDTTSTSEGSNMIGTEETNFDRGYIKKKLEHGLTRIWQDVQLKVKTYLLGTDLSIFKYDDFIFVLDIVSRLMQVGEEFCGSKSEVLQESIRKQSVNYFKNYHRTRLDELRMFLENETWELCPVKSNFSILQLHEFKFMEQSRSPSVSPSKQPASTSSKTVNLFEQYCSGGNPFEIQANHKDEETEDVLASNGYESDEQEKSAYQEYDSDSDVPEELKRDYVDEQTGDAPVKSVSRETLKIRKKSDYSLNKVNAPILTNTTLNVIRLVGKYMQMMNILKPIAFDVIHFMSQLFDYYLYAIYTFFGRNDSLESTGLGLSSSRLRTTLNRIQESLIDLEVSSDPTATLTAAEERKEKVPSPHLSHLVVLTSGDTLYGLAERVVATESLVFLAEQFEFLQPHLDAVMPAVKKPFLQQFYSQTVSTASELRKPIYWIVAGKAIDYEQMLLLMTNVKWDVKEIMSQHNIYVDALLKEFEQFNRRLNEVSKRVRIPLPVSNILWEHCIRLANRTIVEGYANVKKCSNEGRALMQLDFQQFLMKLEKLTDIRPIPDKEFVETYIKAYYLTENDMERWIKEHREYSTKQLTNLVNVCLGSHINKKARQKLLSAIDDIDRPKR

Target ORF information:

RefSeq Version	XM_007522514.2
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus VPS50, EARP/GARPII complex subunit (VPS50), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007522514.2

ORF Insert Sequence:

ATGCAAAAAA TCAAATCTCT TATGACCCGA CAGGGTCTGA GAAGTCCTCC AGAGAACCTC 
AGTGATCTTG GTGCCATTGA GAGTCTCCGG GTCCCTGGAA AGGAGGAATT TCGAGAACTT 
CGAGAACAAC CCAGTGATCC TCAGGCTGAA CAAGAACTAA TTAATAGTAT TGAACAAGTA 
TATTTTTCTA CAGACTCCTT TGATATTGTT AAATATGAGC TGGAGAAACT CCCTCCTATT 
CTCAGTTTGC AAGAACTAGA GGAGTATAGA GACAAATTAA AACAACAGCA AGCTGCGGTC 
TCTAAAAAAG TGGCAGATTT AATTCTTGAA AAACAGCCTG CTTATGTAAA GGTGGGGCAT 
CTAAATATTG CAAAGGAAGG TTTTACTCAA GCTAGTTTAG GCCTTCTTGC AAATCAAAGG 
AAACGCCAGT TGCTGATTGG ACTTCTGAAG TCTCTGAGAA CTATAAAAAC ATTGCAAAGA 
ACAGATGTAC GCTTAAGTGA AATGCTAGAG GAAGAAGACT ACCCAGGAGC TATTCAGCTA 
TGTCTGGAAT GTCAGAAAGC TGCCAGCACT TTCAAACATT ATAGTTGCAT AAGTGAACTA 
AATTCAAAGC TGCAAGATAC TTTAGAACAA ATTGAGGAAC AGTTGGATGT AGCACTTTCC 
AAAATCTGTA AGAATTTTGA CATTAACCAT TACACTAAGG TTCAACAAGC TTATCGACTT 
CTTGGAAAAA CACAGACAGC CATGGATCAA CTTCATATGC ACTTTACCCA GGCCATTCAC 
AATACTGTGT TCCAAGTTGT TCTTGGCTAT GTGGAGTTAT GTGCAGGAAA CACGGATACA 
AAATTCCAAA AACTTCAATA TAAGGATCTC TGTACTCATG TTACACCAGA CAGCTATATT 
CCATGCCTTG CTGACCTGTG CAAAGCACTG TGGGAAGTTA TGCTCAGTTA TTACAGAACT 
ATGGAATGGC ATGAAAAACA TGATAATGAA GATACTACAT CTACTTCTGA AGGGAGTAAT 
ATGATTGGTA CCGAAGAAAC TAATTTTGAT CGTGGCTATA TAAAAAAGAA GTTAGAACAT 
GGACTTACAC GAATATGGCA GGATGTTCAG CTAAAAGTGA AAACCTATTT ACTTGGAACT 
GATTTATCAA TATTCAAATA TGATGACTTC ATCTTTGTTT TGGATATAGT CAGCAGGTTG 
ATGCAAGTTG GAGAAGAATT TTGTGGTAGT AAATCTGAAG TTTTGCAGGA GTCTATTAGG 
AAACAAAGTG TCAATTATTT TAAGAACTAC CATAGAACAC GGCTTGATGA ACTGAGAATG 
TTCCTGGAAA ATGAGACTTG GGAACTTTGT CCCGTTAAGT CTAATTTCAG CATCTTGCAA 
CTTCATGAAT TTAAATTTAT GGAACAGTCT CGTTCCCCAT CTGTTTCACC TAGTAAGCAG 
CCAGCCTCAA CTTCCTCAAA AACAGTTAAC TTGTTTGAGC AGTACTGCAG TGGTGGGAAT 
CCATTTGAAA TTCAGGCCAA CCACAAGGAT GAAGAAACAG AAGATGTTCT GGCTTCTAAT 
GGGTATGAAT CTGATGAACA AGAAAAAAGT GCCTATCAGG AATATGACAG TGACAGTGAT 
GTTCCTGAGG AACTAAAACG GGATTATGTG GATGAACAGA CTGGTGATGC TCCTGTAAAA 
AGTGTTTCTC GAGAAACTCT AAAAATCAGG AAGAAATCCG ATTACAGTCT AAATAAAGTG 
AATGCGCCCA TCTTAACAAA TACAACACTG AATGTAATAA GACTTGTTGG TAAATATATG 
CAGATGATGA ACATTCTTAA GCCAATTGCC TTTGATGTTA TACACTTCAT GTCTCAGCTA 
TTTGACTATT ACTTGTATGC AATCTATACC TTTTTTGGTC GGAATGATTC ATTAGAATCA 
ACAGGACTAG GTCTAAGTAG TAGTAGACTA AGAACAACTC TAAACAGAAT ACAGGAAAGT 
CTTATTGATC TGGAAGTTTC ATCTGACCCT ACTGCCACAC TGACAGCAGC AGAAGAGAGA 
AAGGAGAAGG TTCCAAGCCC ACACCTTAGT CACCTAGTGG TTTTGACATC TGGAGATACA 
TTGTATGGGT TGGCAGAAAG AGTGGTAGCC ACAGAATCCT TGGTATTCTT GGCTGAACAA 
TTTGAGTTCC TTCAGCCACA TCTGGATGCC GTGATGCCTG CGGTCAAAAA GCCCTTTCTT 
CAGCAGTTTT ATTCTCAGAC AGTCTCAACT GCCAGTGAAC TACGCAAACC AATTTACTGG 
ATTGTAGCTG GTAAAGCCAT TGATTATGAA CAAATGCTAC TTCTAATGAC TAATGTGAAA 
TGGGATGTCA AAGAAATTAT GTCACAGCAC AACATATATG TAGATGCATT GCTAAAGGAA 
TTTGAGCAGT TTAACAGGAG GTTAAACGAG GTTTCTAAGA GAGTACGGAT ACCCTTGCCT 
GTGTCTAATA TACTTTGGGA ACATTGTATA CGACTGGCTA ATCGAACTAT TGTGGAAGGA 
TATGCTAATG TCAAGAAGTG TAGCAATGAG GGTCGTGCCT TGATGCAATT AGATTTTCAA 
CAGTTTTTAA TGAAACTGGA AAAACTAACA GATATTAGAC CCATTCCTGA TAAAGAATTT 
GTGGAAACCT ATATTAAAGC CTACTATCTA ACTGAGAATG ACATGGAACG CTGGATCAAA 
GAGCACAGGG AGTATTCAAC CAAGCAGCTG ACCAATCTGG TGAATGTTTG CCTGGGATCC 
CATATCAACA AGAAAGCAAG ACAAAAACTT CTATCAGCCA TAGATGATAT AGACAGGCCC 
AAAAGATAA