VCL cDNA ORF clone, Erinaceus europaeus(western European hedgehog)

Gene
Clones
gRNAs

Summary

Gene Symbol	VCL
Entrez Gene ID	103111104
Full Name	vinculin
Gene Type	protein-coding
Organism	Erinaceus europaeus(western European hedgehog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007520350.2	XP_007520412.1	vinculin isoform X2
XM_007520349.2	XP_007520411.1	vinculin isoform X1

Pathways from BioSystems
Gene Ontology

The following VCL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VCL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEr08218	XM_007520349.2 Latest version!	Erinaceus europaeus vinculin (VCL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OEr08219	XM_007520350.2 Latest version!	Erinaceus europaeus vinculin (VCL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VCL ( XM_007520349.2 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_007520411.1 Erinaceus europaeus vinculin (VCL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OEr08218
Clone ID Related Accession (Same CDS sequence)	XM_007520349.2
Accession Version	XM_007520349.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	vinculin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006804045.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Apr 11, 2016 this sequence version replaced XM_007520349.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCCGTGGC CCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACTGCTCCCG TGGCCGCTGT ACAGGCGGCA GTCAGCAACC TGGTTCGGGT TGGAAAAGAA 
ACTGTTCAAA CCACTGAAGA TCAGATTTTG AAGAGAGACA TGCCACCTGC ATTCATTAAA 
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGATCCATAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTTTC TGGCACCTCA 
GACCTGCTCC TCACCTTCGA TGAGGCAGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTT GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAA ATGGCCAAAA TGATTGATGA GAGACAGCAG 
GAACTCACTC ACCAAGAACA CCGAGTGATG TTGGTGAACT CGATGAATAC CGTGAAAGAG 
TTGCTGCCAG TTCTTATTTC AGCTATGAAG ATATTTGTAA CAACTAAAAA CTCAAAAACA 
CAAGGAATAG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGACAAAAT GAGTGCTGAA 
ATTAATGAAA TCATTCGTGT ATTACAACTT ACTTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAAGATACTG AAGCCATGAA GAGAGCACTG GCCTCCATAG ACTCCAAACT GAATCAGGCC 
AAAGGTTGGC TCTTGAACCC CAGTGCTACC CCAGGGGATG CTGGTGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGAGAG 
ATCCTGGGAA CCTGCAAAGT GTTGGGACAG ATGACTGATC AAGTGGCTGA TCTCCGAGCT 
AGAGGGCAAG GAGCCTCACC CGTGGCCATG CAGAAAGCCC AGCAGGTGTC TCAGGGTCTG 
GATGTGCTCA CAGCAAAGGT GGAAAATGCA GCCCGCAAAC TAGAAGCCAT GACAAACTCA 
AAGCAGAGCA TTGCAAAGAA GATTGATGCC GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGGCCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA GATAGCAGAC 
TTGTGTGATG ATCCTAAAGA GAGAGATGAT ATTCTACGTT CTCTGGGAGA AATAGCTGCT 
CTGACTTCTA AATTAGGAGA TCTACGAAGG CAAGGAAAAG GAGATTCCCC AGAGGCTCGA 
GCACTGGCCA AACAGGTGGC CACAGCCTTG CAGAACTTGC AGACCAAAAC CAATAGGGCT 
GTGGCCAACA GCAGACCCAC CAAAGCAGCA GTCCACCTTG AAGGCAAGAT TGAACAAGCA 
CAGCGCTGGA TTGATAACCC CACAGTAGAT GACCGAGGAG TTGGTCAGGC TGCCATACGG 
GGCCTAGTTG CCGAAGGACA TCGTCTGGCT AATGTCATGA TGGGACCTTA TCGCCAAGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG TTGACTGCCC AGCTGGCTGA TCTGGCGGCT 
AGAGGAGAAG GAGAGAGCCC TCAAGCAAGA GCACTTGCAT CTCAGCTCCA AGAATCTTTA 
AAGGACCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGC 
GATACCACAA CCCCAATCAA GTTGTTGGCG GTGGCAGCCA CTGCGCCTCC TGATGCCCCT 
AATAGAGAAG AGGTATTTGA TGAAAGGGCA GCGAACTTTG AAAACCATTC AGGAAAGCTT 
GGTGCTACAG CAGAGAAGGC AGCTGCTGTT GGAACTGCTA ATAAGTCAAC AGTGGAAGGC 
ATCCAGGCGT CAGTGAAGAC GGCTCGAGAG CTCACACCAC AGGTTGTCTC AGCCGCCCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ACAATGTTGA AAAAATGACA GGACTGGTGG ACGAGGCCAT TGATACCAAA 
TCTCTGTTGG ATGCTTCTGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAAGTAGCC 
ATGGCTAACA TTCAGCCTCA GATGCTGGTC GCTGGGGCAA CAAGCATTGC TCGTAGGGCC 
AACCGGATCC TGCTGGTGGC CAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAACTG AGCAAAACCA TCTCTCCAAT GGTAATGGAT 
GCAAAGGCTG TGGCTGGAAA CATTTCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTGGGAGC GGTGGCCAAG GTTCGAGAAG CCTTCCAGCC TCAGGAGCCT 
GACTTCCCGC CACCTCCTCC AGACCTTGAG CAGCTCCGTC TGACAGATGA ACTTGCTCCC 
CCCAAACCAC CTCTTCCTGA GGGTGAAGTC CCTCCACCCA GGCCCCCACC ACCAGAGGAG 
AAGGATGAAG AGTTCCCTGA GCAGAAGGCC GGGGAGGTCA TTAATCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCATGA TGAAGCCCGG AAGTGGTCCA GCAAGCCGGG TAATCCAGCC 
GCTGAGGTAG GTATAGGTGT TGTAGGAGAG GCAGATGCGG CCGATGCTGT TGGGTTCCCT 
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTATTACC ATCCAGTCAG 
CCGGTCAACC AGCCCATTCT GGCAGCGGCT CAGTCATTGC ATCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GTAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTATT GATGGCTGAG 
ATGTCTCGAT TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTGATTCA GTGTGCCAAG 
GACATCGCCA AGGCCTCAGA TGAGGTGACT CGGTTGGCCA AGGAAGTTGC CAAGCAGTGC 
ACAGATAAGC GGATTAGAAC CAACCTCTTA CAGGTATGTG AGCGAATCCC AACCATCAGT 
ACCCAGCTCA AAATCCTGTC CACAGTGAAA GCCACCATGC TGGGTCGGAC CAATATCAGT 
GATGAAGAAT CTGAACAGGC CACAGAAATG CTGGTTCACA ATGCCCAGAA CCTCATGCAG 
TCCGTGAAAG AGACTGTCCG TGAAGCAGAA GCAGCTTCAA TCAAAATCAG AACAGATGCT 
GGTTTTACAC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007520411.1
CDS	83..3487
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKTQGIEEALKNRNFTVDKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLLNPSATPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKVLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIADLCDDPKERDDILRSLGEIAALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPTKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQESLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGKLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGNPAAEVGIGVVGEADAADAVGFPVPPDMEDDYEPELLLLPSSQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_007520349.2
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus vinculin (VCL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007520349.2

ORF Insert Sequence:

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCCGTGGC CCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACTGCTCCCG TGGCCGCTGT ACAGGCGGCA GTCAGCAACC TGGTTCGGGT TGGAAAAGAA 
ACTGTTCAAA CCACTGAAGA TCAGATTTTG AAGAGAGACA TGCCACCTGC ATTCATTAAA 
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGATCCATAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTTTC TGGCACCTCA 
GACCTGCTCC TCACCTTCGA TGAGGCAGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTT GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAA ATGGCCAAAA TGATTGATGA GAGACAGCAG 
GAACTCACTC ACCAAGAACA CCGAGTGATG TTGGTGAACT CGATGAATAC CGTGAAAGAG 
TTGCTGCCAG TTCTTATTTC AGCTATGAAG ATATTTGTAA CAACTAAAAA CTCAAAAACA 
CAAGGAATAG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGACAAAAT GAGTGCTGAA 
ATTAATGAAA TCATTCGTGT ATTACAACTT ACTTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAAGATACTG AAGCCATGAA GAGAGCACTG GCCTCCATAG ACTCCAAACT GAATCAGGCC 
AAAGGTTGGC TCTTGAACCC CAGTGCTACC CCAGGGGATG CTGGTGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGAGAG 
ATCCTGGGAA CCTGCAAAGT GTTGGGACAG ATGACTGATC AAGTGGCTGA TCTCCGAGCT 
AGAGGGCAAG GAGCCTCACC CGTGGCCATG CAGAAAGCCC AGCAGGTGTC TCAGGGTCTG 
GATGTGCTCA CAGCAAAGGT GGAAAATGCA GCCCGCAAAC TAGAAGCCAT GACAAACTCA 
AAGCAGAGCA TTGCAAAGAA GATTGATGCC GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGGCCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA GATAGCAGAC 
TTGTGTGATG ATCCTAAAGA GAGAGATGAT ATTCTACGTT CTCTGGGAGA AATAGCTGCT 
CTGACTTCTA AATTAGGAGA TCTACGAAGG CAAGGAAAAG GAGATTCCCC AGAGGCTCGA 
GCACTGGCCA AACAGGTGGC CACAGCCTTG CAGAACTTGC AGACCAAAAC CAATAGGGCT 
GTGGCCAACA GCAGACCCAC CAAAGCAGCA GTCCACCTTG AAGGCAAGAT TGAACAAGCA 
CAGCGCTGGA TTGATAACCC CACAGTAGAT GACCGAGGAG TTGGTCAGGC TGCCATACGG 
GGCCTAGTTG CCGAAGGACA TCGTCTGGCT AATGTCATGA TGGGACCTTA TCGCCAAGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG TTGACTGCCC AGCTGGCTGA TCTGGCGGCT 
AGAGGAGAAG GAGAGAGCCC TCAAGCAAGA GCACTTGCAT CTCAGCTCCA AGAATCTTTA 
AAGGACCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGC 
GATACCACAA CCCCAATCAA GTTGTTGGCG GTGGCAGCCA CTGCGCCTCC TGATGCCCCT 
AATAGAGAAG AGGTATTTGA TGAAAGGGCA GCGAACTTTG AAAACCATTC AGGAAAGCTT 
GGTGCTACAG CAGAGAAGGC AGCTGCTGTT GGAACTGCTA ATAAGTCAAC AGTGGAAGGC 
ATCCAGGCGT CAGTGAAGAC GGCTCGAGAG CTCACACCAC AGGTTGTCTC AGCCGCCCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ACAATGTTGA AAAAATGACA GGACTGGTGG ACGAGGCCAT TGATACCAAA 
TCTCTGTTGG ATGCTTCTGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAAGTAGCC 
ATGGCTAACA TTCAGCCTCA GATGCTGGTC GCTGGGGCAA CAAGCATTGC TCGTAGGGCC 
AACCGGATCC TGCTGGTGGC CAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAACTG AGCAAAACCA TCTCTCCAAT GGTAATGGAT 
GCAAAGGCTG TGGCTGGAAA CATTTCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTGGGAGC GGTGGCCAAG GTTCGAGAAG CCTTCCAGCC TCAGGAGCCT 
GACTTCCCGC CACCTCCTCC AGACCTTGAG CAGCTCCGTC TGACAGATGA ACTTGCTCCC 
CCCAAACCAC CTCTTCCTGA GGGTGAAGTC CCTCCACCCA GGCCCCCACC ACCAGAGGAG 
AAGGATGAAG AGTTCCCTGA GCAGAAGGCC GGGGAGGTCA TTAATCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCATGA TGAAGCCCGG AAGTGGTCCA GCAAGCCGGG TAATCCAGCC 
GCTGAGGTAG GTATAGGTGT TGTAGGAGAG GCAGATGCGG CCGATGCTGT TGGGTTCCCT 
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTATTACC ATCCAGTCAG 
CCGGTCAACC AGCCCATTCT GGCAGCGGCT CAGTCATTGC ATCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GTAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTATT GATGGCTGAG 
ATGTCTCGAT TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTGATTCA GTGTGCCAAG 
GACATCGCCA AGGCCTCAGA TGAGGTGACT CGGTTGGCCA AGGAAGTTGC CAAGCAGTGC 
ACAGATAAGC GGATTAGAAC CAACCTCTTA CAGGTATGTG AGCGAATCCC AACCATCAGT 
ACCCAGCTCA AAATCCTGTC CACAGTGAAA GCCACCATGC TGGGTCGGAC CAATATCAGT 
GATGAAGAAT CTGAACAGGC CACAGAAATG CTGGTTCACA ATGCCCAGAA CCTCATGCAG 
TCCGTGAAAG AGACTGTCCG TGAAGCAGAA GCAGCTTCAA TCAAAATCAG AACAGATGCT 
GGTTTTACAC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_007520350.2 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_007520412.1 Erinaceus europaeus vinculin (VCL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OEr08219
Clone ID Related Accession (Same CDS sequence)	XM_007520350.2
Accession Version	XM_007520350.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	vinculin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006804045.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Apr 11, 2016 this sequence version replaced XM_007520350.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCCGTGGC CCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACTGCTCCCG TGGCCGCTGT ACAGGCGGCA GTCAGCAACC TGGTTCGGGT TGGAAAAGAA 
ACTGTTCAAA CCACTGAAGA TCAGATTTTG AAGAGAGACA TGCCACCTGC ATTCATTAAA 
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGATCCATAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTTTC TGGCACCTCA 
GACCTGCTCC TCACCTTCGA TGAGGCAGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTT GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAA ATGGCCAAAA TGATTGATGA GAGACAGCAG 
GAACTCACTC ACCAAGAACA CCGAGTGATG TTGGTGAACT CGATGAATAC CGTGAAAGAG 
TTGCTGCCAG TTCTTATTTC AGCTATGAAG ATATTTGTAA CAACTAAAAA CTCAAAAACA 
CAAGGAATAG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGACAAAAT GAGTGCTGAA 
ATTAATGAAA TCATTCGTGT ATTACAACTT ACTTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAAGATACTG AAGCCATGAA GAGAGCACTG GCCTCCATAG ACTCCAAACT GAATCAGGCC 
AAAGGTTGGC TCTTGAACCC CAGTGCTACC CCAGGGGATG CTGGTGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGAGAG 
ATCCTGGGAA CCTGCAAAGT GTTGGGACAG ATGACTGATC AAGTGGCTGA TCTCCGAGCT 
AGAGGGCAAG GAGCCTCACC CGTGGCCATG CAGAAAGCCC AGCAGGTGTC TCAGGGTCTG 
GATGTGCTCA CAGCAAAGGT GGAAAATGCA GCCCGCAAAC TAGAAGCCAT GACAAACTCA 
AAGCAGAGCA TTGCAAAGAA GATTGATGCC GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGGCCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA GATAGCAGAC 
TTGTGTGATG ATCCTAAAGA GAGAGATGAT ATTCTACGTT CTCTGGGAGA AATAGCTGCT 
CTGACTTCTA AATTAGGAGA TCTACGAAGG CAAGGAAAAG GAGATTCCCC AGAGGCTCGA 
GCACTGGCCA AACAGGTGGC CACAGCCTTG CAGAACTTGC AGACCAAAAC CAATAGGGCT 
GTGGCCAACA GCAGACCCAC CAAAGCAGCA GTCCACCTTG AAGGCAAGAT TGAACAAGCA 
CAGCGCTGGA TTGATAACCC CACAGTAGAT GACCGAGGAG TTGGTCAGGC TGCCATACGG 
GGCCTAGTTG CCGAAGGACA TCGTCTGGCT AATGTCATGA TGGGACCTTA TCGCCAAGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG TTGACTGCCC AGCTGGCTGA TCTGGCGGCT 
AGAGGAGAAG GAGAGAGCCC TCAAGCAAGA GCACTTGCAT CTCAGCTCCA AGAATCTTTA 
AAGGACCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGC 
GATACCACAA CCCCAATCAA GTTGTTGGCG GTGGCAGCCA CTGCGCCTCC TGATGCCCCT 
AATAGAGAAG AGGTATTTGA TGAAAGGGCA GCGAACTTTG AAAACCATTC AGGAAAGCTT 
GGTGCTACAG CAGAGAAGGC AGCTGCTGTT GGAACTGCTA ATAAGTCAAC AGTGGAAGGC 
ATCCAGGCGT CAGTGAAGAC GGCTCGAGAG CTCACACCAC AGGTTGTCTC AGCCGCCCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ACAATGTTGA AAAAATGACA GGACTGGTGG ACGAGGCCAT TGATACCAAA 
TCTCTGTTGG ATGCTTCTGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAAGTAGCC 
ATGGCTAACA TTCAGCCTCA GATGCTGGTC GCTGGGGCAA CAAGCATTGC TCGTAGGGCC 
AACCGGATCC TGCTGGTGGC CAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAACTG AGCAAAACCA TCTCTCCAAT GGTAATGGAT 
GCAAAGGCTG TGGCTGGAAA CATTTCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTGGGAGC GGTGGCCAAG GTTCGAGAAG CCTTCCAGCC TCAGGAGCCT 
GACTTCCCGC CACCTCCTCC AGACCTTGAG CAGCTCCGTC TGACAGATGA ACTTGCTCCC 
CCCAAACCAC CTCTTCCTGA GGGTGAAGTC CCTCCACCCA GGCCCCCACC ACCAGAGGAG 
AAGGATGAAG AGTTCCCTGA GCAGAAGGCC GGGGAGGTCA TTAATCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCATGA TGAAGCCCGG AAGTGGTCCA GCAAGGGTAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTATTGATG GCTGAGATGT CTCGATTGGT AAGAGGGGGC 
AGTGGTACCA AGCGGGCACT GATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGATGAG 
GTGACTCGGT TGGCCAAGGA AGTTGCCAAG CAGTGCACAG ATAAGCGGAT TAGAACCAAC 
CTCTTACAGG TATGTGAGCG AATCCCAACC ATCAGTACCC AGCTCAAAAT CCTGTCCACA 
GTGAAAGCCA CCATGCTGGG TCGGACCAAT ATCAGTGATG AAGAATCTGA ACAGGCCACA 
GAAATGCTGG TTCACAATGC CCAGAACCTC ATGCAGTCCG TGAAAGAGAC TGTCCGTGAA 
GCAGAAGCAG CTTCAATCAA AATCAGAACA GATGCTGGTT TTACACTGCG CTGGGTTAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007520412.1
CDS	83..3283
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKTQGIEEALKNRNFTVDKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLLNPSATPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKVLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIADLCDDPKERDDILRSLGEIAALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPTKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQESLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGKLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_007520350.2
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus vinculin (VCL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007520350.2

ORF Insert Sequence:

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCCGTGGC CCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACTGCTCCCG TGGCCGCTGT ACAGGCGGCA GTCAGCAACC TGGTTCGGGT TGGAAAAGAA 
ACTGTTCAAA CCACTGAAGA TCAGATTTTG AAGAGAGACA TGCCACCTGC ATTCATTAAA 
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGATCCATAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTTTC TGGCACCTCA 
GACCTGCTCC TCACCTTCGA TGAGGCAGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTT GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAA ATGGCCAAAA TGATTGATGA GAGACAGCAG 
GAACTCACTC ACCAAGAACA CCGAGTGATG TTGGTGAACT CGATGAATAC CGTGAAAGAG 
TTGCTGCCAG TTCTTATTTC AGCTATGAAG ATATTTGTAA CAACTAAAAA CTCAAAAACA 
CAAGGAATAG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGACAAAAT GAGTGCTGAA 
ATTAATGAAA TCATTCGTGT ATTACAACTT ACTTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAAGATACTG AAGCCATGAA GAGAGCACTG GCCTCCATAG ACTCCAAACT GAATCAGGCC 
AAAGGTTGGC TCTTGAACCC CAGTGCTACC CCAGGGGATG CTGGTGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGAGAG 
ATCCTGGGAA CCTGCAAAGT GTTGGGACAG ATGACTGATC AAGTGGCTGA TCTCCGAGCT 
AGAGGGCAAG GAGCCTCACC CGTGGCCATG CAGAAAGCCC AGCAGGTGTC TCAGGGTCTG 
GATGTGCTCA CAGCAAAGGT GGAAAATGCA GCCCGCAAAC TAGAAGCCAT GACAAACTCA 
AAGCAGAGCA TTGCAAAGAA GATTGATGCC GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGGCCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA GATAGCAGAC 
TTGTGTGATG ATCCTAAAGA GAGAGATGAT ATTCTACGTT CTCTGGGAGA AATAGCTGCT 
CTGACTTCTA AATTAGGAGA TCTACGAAGG CAAGGAAAAG GAGATTCCCC AGAGGCTCGA 
GCACTGGCCA AACAGGTGGC CACAGCCTTG CAGAACTTGC AGACCAAAAC CAATAGGGCT 
GTGGCCAACA GCAGACCCAC CAAAGCAGCA GTCCACCTTG AAGGCAAGAT TGAACAAGCA 
CAGCGCTGGA TTGATAACCC CACAGTAGAT GACCGAGGAG TTGGTCAGGC TGCCATACGG 
GGCCTAGTTG CCGAAGGACA TCGTCTGGCT AATGTCATGA TGGGACCTTA TCGCCAAGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG TTGACTGCCC AGCTGGCTGA TCTGGCGGCT 
AGAGGAGAAG GAGAGAGCCC TCAAGCAAGA GCACTTGCAT CTCAGCTCCA AGAATCTTTA 
AAGGACCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGC 
GATACCACAA CCCCAATCAA GTTGTTGGCG GTGGCAGCCA CTGCGCCTCC TGATGCCCCT 
AATAGAGAAG AGGTATTTGA TGAAAGGGCA GCGAACTTTG AAAACCATTC AGGAAAGCTT 
GGTGCTACAG CAGAGAAGGC AGCTGCTGTT GGAACTGCTA ATAAGTCAAC AGTGGAAGGC 
ATCCAGGCGT CAGTGAAGAC GGCTCGAGAG CTCACACCAC AGGTTGTCTC AGCCGCCCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ACAATGTTGA AAAAATGACA GGACTGGTGG ACGAGGCCAT TGATACCAAA 
TCTCTGTTGG ATGCTTCTGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAAGTAGCC 
ATGGCTAACA TTCAGCCTCA GATGCTGGTC GCTGGGGCAA CAAGCATTGC TCGTAGGGCC 
AACCGGATCC TGCTGGTGGC CAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAACTG AGCAAAACCA TCTCTCCAAT GGTAATGGAT 
GCAAAGGCTG TGGCTGGAAA CATTTCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTGGGAGC GGTGGCCAAG GTTCGAGAAG CCTTCCAGCC TCAGGAGCCT 
GACTTCCCGC CACCTCCTCC AGACCTTGAG CAGCTCCGTC TGACAGATGA ACTTGCTCCC 
CCCAAACCAC CTCTTCCTGA GGGTGAAGTC CCTCCACCCA GGCCCCCACC ACCAGAGGAG 
AAGGATGAAG AGTTCCCTGA GCAGAAGGCC GGGGAGGTCA TTAATCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCATGA TGAAGCCCGG AAGTGGTCCA GCAAGGGTAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTATTGATG GCTGAGATGT CTCGATTGGT AAGAGGGGGC 
AGTGGTACCA AGCGGGCACT GATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGATGAG 
GTGACTCGGT TGGCCAAGGA AGTTGCCAAG CAGTGCACAG ATAAGCGGAT TAGAACCAAC 
CTCTTACAGG TATGTGAGCG AATCCCAACC ATCAGTACCC AGCTCAAAAT CCTGTCCACA 
GTGAAAGCCA CCATGCTGGG TCGGACCAAT ATCAGTGATG AAGAATCTGA ACAGGCCACA 
GAAATGCTGG TTCACAATGC CCAGAACCTC ATGCAGTCCG TGAAAGAGAC TGTCCGTGAA 
GCAGAAGCAG CTTCAATCAA AATCAGAACA GATGCTGGTT TTACACTGCG CTGGGTTAGA 
AAGACTCCCT GGTACCAGTA G