NOS3 cDNA ORF clone, Equus caballus(horse)

The following NOS3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHb64511 XM_001504650.6
Latest version!
Equus caballus nitric oxide synthase 3 (NOS3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHb20116 XM_001504650.5
Latest version!
Equus caballus nitric oxide synthase 3 (endothelial cell) (NOS3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHb64511
    Clone ID Related Accession (Same CDS sequence) XM_001504650.6
    Accession Version XM_001504650.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3615bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product nitric oxide synthase, endothelial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009147.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_001504650.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCTGGGCCAC CCTGCGGCCT GGGGCTGGGG 
    CTGGGCCTCG GGCTGTGTGG CAAGCAGGGC CCAGCCTCCC CCGCATCTGA GCCCAGCCGG
    GCACCTGTAC CCGCACCCGC ACCCGCACCC CTGCCTGCGC CAGACCACAG CCCCCCGCTC
    GCCAGGCCCC CAGAGGGGCC CAAGTTCCCG CGTGTGAAGA ACTGGGAGGT GGGGAGCATC
    ACCTACGACA CCCTGTGTGC CCAGTCCCAG CAGGATGGGC CCTGCACCCC AAGACGCTGC
    CTCGGCTCCC TGGTGTTTCC ACGGAAACTG CAGAGCCGGC CGTCCCAGGG CCCTCCGCCT
    CCTGAGCAGC TGCTGAGTCA GGCTAGGGAC TTCATCAACC AGTACTACAG CTCCATCAAG
    AGGAGCGGCT CCCAGGCCCA CGAGCAGCGT CTTCAGGAGG TGGAGGCCGA GGTGGCAGCC
    ACGGGCACCT ACCAGCTTCG GGAGAGTGAG CTGGTATTTG GGGCCAAGCA GGCCTGGCGC
    AACGCTCCCC GTTGTGTGGG CCGGATCCAG TGGGGGAAAC TGCAGGTCTT TGATGCGCGG
    GATTGCAGCT CTGCGCAGGA GATGTTCACC TGCATATGCA ACCACATCAA GTACGCCACC
    AACCGGGGCA ACCTTCGCTC TGCCATCACA GTGTTCCCCC AGCGCACCCC GGGCCGCGGA
    GACTTCAGAA TCTGGAACAG CCAACTGGTG CGCTATGCAG GCTACAGACA GCAGGACGGC
    TCTGTGCGGG GGGACCCAGC CAACGTGGAG ATCACCGAGC TCTGCGTCCA GCATGGCTGG
    ACTCCAGGAA ATGGCCGCTT TGATGTGCTG CCCCTGCTGC TCCAGGCCCC AGATGAACCC
    CCGGAACTCT TCACTCTGCC CCCCGAGCTG GTGCTTGAAG TGCCCCTGGA GCACCCCACG
    CTGGAGTGGT TCGCATCCCT GGGCCTGCGC TGGTACGCGC TCCCGGCAGT GTCCAACATG
    CTGCTGGAAA TCGGGGGCCT GGAGTTCCCT GCGGCCCCTT TCAGCGGCTG GTACATGAGC
    ACTGAGATCG GCACGCGGAA CCTGTGTGAC CCTCACCGCT ACAACATCCT GGAGGATGTG
    GCCGTCTGCA TGGACTTGGA TACCAGGACA ACCTCGTCCC TGTGGAAAGA CAAGGCAGCG
    GTGGAAATCA ACTTGGCTGT GCTGCACAGT TACCAGCTGG CCAAAGTGAC CATTGTGGAC
    CACCACGCCG CCACAGCCTC CTTCATGAAG CACCTGGAGA ATGAGCAGAA GGCGAGGGGG
    GGCTGCCCTG CCGACTGGGC CTGGATTGTG CCCCCCATCT CGGGCAGCCT CACCCCTGTC
    TTCCATCAGG AGATGGTCAA CTATGTCCTG TCCCCTGCCT TCCGCTACCA GCCAGACCCC
    TGGAAGGGGA GTGCAGTCAA GGGCACCGGC CTCGCCAGGA AGAAGACCTT TAAGGAAGTG
    GCCAATGCTG TGAAGATCTC TGCCTCCCTC ATGGGCACCG TGATGGCAAA GAGAGTGAAG
    GCTACAATCC TGTACGGCTC CGAGACCGGC CGGGCCCAGG GCTACGCACA GCAGCTGGGG
    AGGCTCTTCC GGAAGGCTTT CGACCCCCGG GTCCTGTGCA TGGATGAGTA TGATGTGGTG
    TCCCTCGAGC ACGAGACACT GGTGTTGGTG GTGACCAGCA CATTCGGGAA TGGGGATCCC
    CCAGAGAATG GAGAGAGTTT CGCAGCAGCC CTGATGGAGA TGTCGGGCCC TTACAACAGC
    TCCCCTCGGC CAGAACAGCA CAAGAGTTAC AAAATCCGCT TCAACAGCGT CTCCTGTTCA
    GACCCACTGG TGTCCTCCTG GCGGCGGAAG AGAAAGGAAT CCAGTAACAC AGACAGTGCA
    GGGGCACTGG GGACCCTCAG GTTCTGTGTG TTCGGGCTCG GCTCCCGGGC GTACCCGCAC
    TTCTGCGCCT TTGCTCGTGC GGTGGACACG CGGCTGGAAG AGCTGGGTGG CGAGCGGCTC
    CTGCAGCTGG GCCAGGGAGA CGAGCTGTGC GGCCAGGAGG AGGCCTTCCG TGGCTGGGCC
    CAAGCCGCCT TCCAGGCCTC CTGTGAGACC TTCTGCGTGG GAGAGGATGC CAAGGCTGCT
    GCCCGGGACA TATTCAGCCC CAAACGGAGC TGGAAGCGCC AGAGGTACCG GCTGAGCACC
    CAGGCTGAGG GCCTCCAGTT GCTACCAGGC CTGATCCATG TGCACCGGCG AAAGATGTTC
    CAGGCTACAG TCCTTTCAGT GGAAAATCTG CAAAGCAGCA AGTCCACCCG GGCCACGATC
    CTGGTGCGCC TGGACACTGG AGGCCAGGAG GGGCTCCAGT ACCAGCCAGG GGACCACATA
    GGCGTCTGCC CACCCAACCG GCCGGGACTC GTGGAGGCAC TGCTCAGCCG TGTGGAGGAC
    CCGCCGCCAG CAGGCGAGCC CGTGGCAGTG GAACAGCTGG AGAAGGGCAG CCCTGGTGGC
    CCTCCCCCAA GCTGGGTGCG GGACCCCCGG CTGCCCCCGT GCACGCTGCG CCAGGCTCTC
    ACCTTCTTCC TGGACATCAC TTCCCCGCCC AGCCCTCAAC TCCTTCGACT GCTCAGCACC
    TTGGCAGAAG AACCCAGTGA ACAGCAGGAG CTTGAGACCC TCAGCCAGGA CCCCCGGCGC
    TACGAAGAAT GGAAGTGGTT CCGCTGCCCC ACGCTGCTGG AGGTGCTGGA GCAGTTTCCT
    TCGGTGGCAC TGCCTGCTGC CCTGCTCCTC ACCCAGCTGC CCCTGCTCCA GCCCCGGTAC
    TACTCCGTCA GCTCAGCCCC CAGCACCCAC CCAGGAGAGA TCCACCTCAC AGTTGCCGTG
    CTGGCATACA GGACCCAGGA TGGGCTAGGC CCCCTGCACT ATGGAGTCTG CTCCACATGG
    CTGAGCCAAC TCAAGGCCGG AGACCTTGTA CCCTGCTTCA TCAGGGGGGC TCCCTCCTTC
    CGGCTGCCTC CGGACCCCAG CTTGCCCTGC ATCCTGGTGG GCCCCGGCAC GGGCATCGCC
    CCCTTCCGGG GATTTTGGCA GGAGCGGCTG CATGACATTG AGAGCAAAGG GCTGCAGCCC
    GCCCCCATGA CCTTGGTGTT CGGCTGCCGA TGCTCCCAGC TCGATCACCT CTACCGCGAC
    GAGGTGCAGG ATGCCCAGCA GCGCGGGGTA TTTGGCCGCG TCCTCACCGC CTTCTCCAGG
    GAACCGGACA GCCCTAAGAC CTACGTACAG GACATCCTGC GGACCGAGCT GGCTGCCGAG
    GTGCACCGCG TGCTGTGCCT CGATCGGGGC CACATGTTTG TCTGCGGTGA TGTCACCATG
    GCAACCAGCG TCTTGCAGAC GGTGCAACGC ATCCTGGCGA CAGAGGGCGA CATGGAACTG
    GACGAGGCCG GCGACGTCAT CGGCGTGCTG CGGGATCAGC AACGCTATCA CGAGGACATT
    TTCGGGCTCA CGCTGCGCAC CCAGGAGGTG ACAAGCCGCA TACGCACCCA GAGCCTTTCC
    TTGCACGAGC GGCATCTGCA GGGCACAGTG CCCTGGGCGT TCGACCCGCC CGGCCCAGAC
    ACCTCTGGTC GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001504700.4
    CDS49..3663
    Translation

    Target ORF information:

    RefSeq Version XM_001504650.6
    Organism Equus caballus(horse)
    Definition Equus caballus nitric oxide synthase 3 (NOS3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001504650.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCTGGGCCAC CCTGCGGCCT GGGGCTGGGG 
    CTGGGCCTCG GGCTGTGTGG CAAGCAGGGC CCAGCCTCCC CCGCATCTGA GCCCAGCCGG
    GCACCTGTAC CCGCACCCGC ACCCGCACCC CTGCCTGCGC CAGACCACAG CCCCCCGCTC
    GCCAGGCCCC CAGAGGGGCC CAAGTTCCCG CGTGTGAAGA ACTGGGAGGT GGGGAGCATC
    ACCTACGACA CCCTGTGTGC CCAGTCCCAG CAGGATGGGC CCTGCACCCC AAGACGCTGC
    CTCGGCTCCC TGGTGTTTCC ACGGAAACTG CAGAGCCGGC CGTCCCAGGG CCCTCCGCCT
    CCTGAGCAGC TGCTGAGTCA GGCTAGGGAC TTCATCAACC AGTACTACAG CTCCATCAAG
    AGGAGCGGCT CCCAGGCCCA CGAGCAGCGT CTTCAGGAGG TGGAGGCCGA GGTGGCAGCC
    ACGGGCACCT ACCAGCTTCG GGAGAGTGAG CTGGTATTTG GGGCCAAGCA GGCCTGGCGC
    AACGCTCCCC GTTGTGTGGG CCGGATCCAG TGGGGGAAAC TGCAGGTCTT TGATGCGCGG
    GATTGCAGCT CTGCGCAGGA GATGTTCACC TGCATATGCA ACCACATCAA GTACGCCACC
    AACCGGGGCA ACCTTCGCTC TGCCATCACA GTGTTCCCCC AGCGCACCCC GGGCCGCGGA
    GACTTCAGAA TCTGGAACAG CCAACTGGTG CGCTATGCAG GCTACAGACA GCAGGACGGC
    TCTGTGCGGG GGGACCCAGC CAACGTGGAG ATCACCGAGC TCTGCGTCCA GCATGGCTGG
    ACTCCAGGAA ATGGCCGCTT TGATGTGCTG CCCCTGCTGC TCCAGGCCCC AGATGAACCC
    CCGGAACTCT TCACTCTGCC CCCCGAGCTG GTGCTTGAAG TGCCCCTGGA GCACCCCACG
    CTGGAGTGGT TCGCATCCCT GGGCCTGCGC TGGTACGCGC TCCCGGCAGT GTCCAACATG
    CTGCTGGAAA TCGGGGGCCT GGAGTTCCCT GCGGCCCCTT TCAGCGGCTG GTACATGAGC
    ACTGAGATCG GCACGCGGAA CCTGTGTGAC CCTCACCGCT ACAACATCCT GGAGGATGTG
    GCCGTCTGCA TGGACTTGGA TACCAGGACA ACCTCGTCCC TGTGGAAAGA CAAGGCAGCG
    GTGGAAATCA ACTTGGCTGT GCTGCACAGT TACCAGCTGG CCAAAGTGAC CATTGTGGAC
    CACCACGCCG CCACAGCCTC CTTCATGAAG CACCTGGAGA ATGAGCAGAA GGCGAGGGGG
    GGCTGCCCTG CCGACTGGGC CTGGATTGTG CCCCCCATCT CGGGCAGCCT CACCCCTGTC
    TTCCATCAGG AGATGGTCAA CTATGTCCTG TCCCCTGCCT TCCGCTACCA GCCAGACCCC
    TGGAAGGGGA GTGCAGTCAA GGGCACCGGC CTCGCCAGGA AGAAGACCTT TAAGGAAGTG
    GCCAATGCTG TGAAGATCTC TGCCTCCCTC ATGGGCACCG TGATGGCAAA GAGAGTGAAG
    GCTACAATCC TGTACGGCTC CGAGACCGGC CGGGCCCAGG GCTACGCACA GCAGCTGGGG
    AGGCTCTTCC GGAAGGCTTT CGACCCCCGG GTCCTGTGCA TGGATGAGTA TGATGTGGTG
    TCCCTCGAGC ACGAGACACT GGTGTTGGTG GTGACCAGCA CATTCGGGAA TGGGGATCCC
    CCAGAGAATG GAGAGAGTTT CGCAGCAGCC CTGATGGAGA TGTCGGGCCC TTACAACAGC
    TCCCCTCGGC CAGAACAGCA CAAGAGTTAC AAAATCCGCT TCAACAGCGT CTCCTGTTCA
    GACCCACTGG TGTCCTCCTG GCGGCGGAAG AGAAAGGAAT CCAGTAACAC AGACAGTGCA
    GGGGCACTGG GGACCCTCAG GTTCTGTGTG TTCGGGCTCG GCTCCCGGGC GTACCCGCAC
    TTCTGCGCCT TTGCTCGTGC GGTGGACACG CGGCTGGAAG AGCTGGGTGG CGAGCGGCTC
    CTGCAGCTGG GCCAGGGAGA CGAGCTGTGC GGCCAGGAGG AGGCCTTCCG TGGCTGGGCC
    CAAGCCGCCT TCCAGGCCTC CTGTGAGACC TTCTGCGTGG GAGAGGATGC CAAGGCTGCT
    GCCCGGGACA TATTCAGCCC CAAACGGAGC TGGAAGCGCC AGAGGTACCG GCTGAGCACC
    CAGGCTGAGG GCCTCCAGTT GCTACCAGGC CTGATCCATG TGCACCGGCG AAAGATGTTC
    CAGGCTACAG TCCTTTCAGT GGAAAATCTG CAAAGCAGCA AGTCCACCCG GGCCACGATC
    CTGGTGCGCC TGGACACTGG AGGCCAGGAG GGGCTCCAGT ACCAGCCAGG GGACCACATA
    GGCGTCTGCC CACCCAACCG GCCGGGACTC GTGGAGGCAC TGCTCAGCCG TGTGGAGGAC
    CCGCCGCCAG CAGGCGAGCC CGTGGCAGTG GAACAGCTGG AGAAGGGCAG CCCTGGTGGC
    CCTCCCCCAA GCTGGGTGCG GGACCCCCGG CTGCCCCCGT GCACGCTGCG CCAGGCTCTC
    ACCTTCTTCC TGGACATCAC TTCCCCGCCC AGCCCTCAAC TCCTTCGACT GCTCAGCACC
    TTGGCAGAAG AACCCAGTGA ACAGCAGGAG CTTGAGACCC TCAGCCAGGA CCCCCGGCGC
    TACGAAGAAT GGAAGTGGTT CCGCTGCCCC ACGCTGCTGG AGGTGCTGGA GCAGTTTCCT
    TCGGTGGCAC TGCCTGCTGC CCTGCTCCTC ACCCAGCTGC CCCTGCTCCA GCCCCGGTAC
    TACTCCGTCA GCTCAGCCCC CAGCACCCAC CCAGGAGAGA TCCACCTCAC AGTTGCCGTG
    CTGGCATACA GGACCCAGGA TGGGCTAGGC CCCCTGCACT ATGGAGTCTG CTCCACATGG
    CTGAGCCAAC TCAAGGCCGG AGACCTTGTA CCCTGCTTCA TCAGGGGGGC TCCCTCCTTC
    CGGCTGCCTC CGGACCCCAG CTTGCCCTGC ATCCTGGTGG GCCCCGGCAC GGGCATCGCC
    CCCTTCCGGG GATTTTGGCA GGAGCGGCTG CATGACATTG AGAGCAAAGG GCTGCAGCCC
    GCCCCCATGA CCTTGGTGTT CGGCTGCCGA TGCTCCCAGC TCGATCACCT CTACCGCGAC
    GAGGTGCAGG ATGCCCAGCA GCGCGGGGTA TTTGGCCGCG TCCTCACCGC CTTCTCCAGG
    GAACCGGACA GCCCTAAGAC CTACGTACAG GACATCCTGC GGACCGAGCT GGCTGCCGAG
    GTGCACCGCG TGCTGTGCCT CGATCGGGGC CACATGTTTG TCTGCGGTGA TGTCACCATG
    GCAACCAGCG TCTTGCAGAC GGTGCAACGC ATCCTGGCGA CAGAGGGCGA CATGGAACTG
    GACGAGGCCG GCGACGTCAT CGGCGTGCTG CGGGATCAGC AACGCTATCA CGAGGACATT
    TTCGGGCTCA CGCTGCGCAC CCAGGAGGTG ACAAGCCGCA TACGCACCCA GAGCCTTTCC
    TTGCACGAGC GGCATCTGCA GGGCACAGTG CCCTGGGCGT TCGACCCGCC CGGCCCAGAC
    ACCTCTGGTC GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb20116
    Clone ID Related Accession (Same CDS sequence) XM_001504650.5
    Accession Version XM_001504650.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3615bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product nitric oxide synthase, endothelial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867415.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_001504650.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCTGGGCCAC CCTGCGGCCT GGGGCTGGGG 
    CTGGGCCTCG GGCTGTGTGG CAAGCAGGGC CCAGCCTCCC CCGCATCTGA GCCCAGCCGG
    GCACCTGTAC CCGCACCCGC ACCCGCACCC CTGCCTGCGC CAGACCACAG CCCCCCGCTC
    GCCAGGCCCC CAGAGGGGCC CAAGTTCCCG CGTGTGAAGA ACTGGGAGGT GGGGAGCATC
    ACCTACGACA CCCTGTGTGC CCAGTCCCAG CAGGATGGGC CCTGCACCCC AAGACGCTGC
    CTCGGCTCCC TGGTGTTTCC ACGGAAACTG CAGAGCCGGC CGTCCCAGGG CCCTCCGCCT
    CCTGAGCAGC TGCTGAGTCA GGCTAGGGAC TTCATCAACC AGTACTACAG CTCCATCAAG
    AGGAGCGGCT CCCAGGCCCA CGAGCAGCGT CTTCAGGAGG TGGAGGCCGA GGTGGCAGCC
    ACGGGCACCT ACCAGCTTCG GGAGAGTGAG CTGGTATTTG GGGCCAAGCA GGCCTGGCGC
    AACGCTCCCC GTTGTGTGGG CCGGATCCAG TGGGGGAAAC TGCAGGTCTT TGATGCGCGG
    GATTGCAGCT CTGCGCAGGA GATGTTCACC TGCATATGCA ACCACATCAA GTACGCCACC
    AACCGGGGCA ACCTTCGCTC TGCCATCACA GTGTTCCCCC AGCGCACCCC GGGCCGCGGA
    GACTTCAGAA TCTGGAACAG CCAACTGGTG CGCTATGCAG GCTACAGACA GCAGGACGGC
    TCTGTGCGGG GGGACCCAGC CAACGTGGAG ATCACCGAGC TCTGCGTCCA GCATGGCTGG
    ACTCCAGGAA ATGGCCGCTT TGATGTGCTG CCCCTGCTGC TCCAGGCCCC AGATGAACCC
    CCGGAACTCT TCACTCTGCC CCCCGAGCTG GTGCTTGAAG TGCCCCTGGA GCACCCCACG
    CTGGAGTGGT TCGCATCCCT GGGCCTGCGC TGGTACGCGC TCCCGGCAGT GTCCAACATG
    CTGCTGGAAA TCGGGGGCCT GGAGTTCCCT GCGGCCCCTT TCAGCGGCTG GTACATGAGC
    ACTGAGATCG GCACGCGGAA CCTGTGTGAC CCTCACCGCT ACAACATCCT GGAGGATGTG
    GCCGTCTGCA TGGACTTGGA TACCAGGACA ACCTCGTCCC TGTGGAAAGA CAAGGCAGCG
    GTGGAAATCA ACTTGGCTGT GCTGCACAGT TACCAGCTGG CCAAAGTGAC CATTGTGGAC
    CACCACGCCG CCACAGCCTC CTTCATGAAG CACCTGGAGA ATGAGCAGAA GGCGAGGGGG
    GGCTGCCCTG CCGACTGGGC CTGGATTGTG CCCCCCATCT CGGGCAGCCT CACCCCTGTC
    TTCCATCAGG AGATGGTCAA CTATGTCCTG TCCCCTGCCT TCCGCTACCA GCCAGACCCC
    TGGAAGGGGA GTGCAGTCAA GGGCACCGGC CTCGCCAGGA AGAAGACCTT TAAGGAAGTG
    GCCAATGCTG TGAAGATCTC TGCCTCCCTC ATGGGCACCG TGATGGCAAA GAGAGTGAAG
    GCTACAATCC TGTACGGCTC CGAGACCGGC CGGGCCCAGG GCTACGCACA GCAGCTGGGG
    AGGCTCTTCC GGAAGGCTTT CGACCCCCGG GTCCTGTGCA TGGATGAGTA TGATGTGGTG
    TCCCTCGAGC ACGAGACACT GGTGTTGGTG GTGACCAGCA CATTCGGGAA TGGGGATCCC
    CCAGAGAATG GAGAGAGTTT CGCAGCAGCC CTGATGGAGA TGTCGGGCCC TTACAACAGC
    TCCCCTCGGC CAGAACAGCA CAAGAGTTAC AAAATCCGCT TCAACAGCGT CTCCTGTTCA
    GACCCACTGG TGTCCTCCTG GCGGCGGAAG AGAAAGGAAT CCAGTAACAC AGACAGTGCA
    GGGGCACTGG GGACCCTCAG GTTCTGTGTG TTCGGGCTCG GCTCCCGGGC GTACCCGCAC
    TTCTGCGCCT TTGCTCGTGC GGTGGACACG CGGCTGGAAG AGCTGGGTGG CGAGCGGCTC
    CTGCAGCTGG GCCAGGGAGA CGAGCTGTGC GGCCAGGAGG AGGCCTTCCG TGGCTGGGCC
    CAAGCCGCCT TCCAGGCCTC CTGTGAGACC TTCTGCGTGG GAGAGGATGC CAAGGCTGCT
    GCCCGGGACA TATTCAGCCC CAAACGGAGC TGGAAGCGCC AGAGGTACCG GCTGAGCACC
    CAGGCTGAGG GCCTCCAGTT GCTACCAGGC CTGATCCATG TGCACCGGCG AAAGATGTTC
    CAGGCTACAG TCCTTTCAGT GGAAAATCTG CAAAGCAGCA GGTCCACCCG GGCCACGATC
    CTGGTGCGCC TGGACACTGG AGGCCAGGAG GGGCTCCAGT ACCAGCCAGG GGACCACATA
    GGCGTCTGCC CACCCAACCG GCCGGGACTC GTGGAGGCAC TGCTCAGCCG TGTGGAGGAC
    CCGCCGCCAG CAGGCGAGCC CGTGGCAGTG GAACAGCTGG AGAAGGGCAG CCCTGGTGGC
    CCTCCCCCAA GCTGGGTGCG GGACCCCCGG CTGCCCCCGT GCACGCTGCG CCAGGCTCTC
    ACCTTCTTCC TGGACATCAC TTCCCCGCCC AGCCCTCAAC TCCTTCGACT GCTCAGCACC
    TTGGCAGAAG AACCCAGTGA ACAGCAGGAG CTTGAGACCC TCAGCCAGGA CCCCCGGCGC
    TACGAAGAAT GGAAGTGGTT CCGCTGCCCC ACGCTGCTGG AGGTGCTGGA GCAGTTTCCT
    TCGGTGGCAC TGCCTGCTGC CCTGCTCCTC ACCCAGCTGC CCCTGCTCCA GCCCCGGTAC
    TACTCCGTCA GCTCAGCCCC CAGCACCCAC CCAGGAGAGA TCCACCTCAC AGTTGCCGTG
    CTGGCATACA GGACCCAGGA TGGGCTAGGC CCCCTGCACT ATGGAGTCTG CTCCACATGG
    CTGAGCCAAC TCAAGGCCGG AGACCTTGTA CCCTGCTTCA TCAGGGGGGC TCCCTCCTTC
    CGGCTGCCTC CGGACCCCAG CTTGCCCTGC ATCCTGGTGG GCCCCGGCAC GGGCATCGCC
    CCCTTCCGGG GATTTTGGCA GGAGCGGCTG CATGACATTG AGAGCAAAGG GCTGCAGCCC
    GCCCCCATGA CCTTGGTGTT CGGCTGCCGA TGCTCCCAGC TCGATCACCT CTACCGCGAC
    GAGGTGCAGG ATGCCCAGCA GCGCGGGGTA TTTGGCCGCG TCCTCACCGC CTTCTCCAGG
    GAACCGGACA GCCCTAAGAC CTACGTACAG GACATCCTGC GGACCGAGCT GGCTGCCGAG
    GTGCACCGCG TGCTGTGCCT CGATCGGGGC CACATGTTTG TCTGCGGTGA TGTCACCATG
    GCAACCAGCG TCTTGCAGAC GGTGCAACGC ATCCTGGCGA CAGAGGGCGA CATGGAACTG
    GACGAGGCCG GCGACGTCAT CGGCGTGCTG CGGGATCAGC AACGCTATCA CGAGGACATT
    TTCGGGCTCA CGCTGCGCAC CCAGGAGGTG ACAAGCCGCA TACGCACCCA GAGCCTTTCC
    TTGCACGAGC GGCATCTGCA GGGCACAGTG CCCTGGGCGT TCGACCCGCC CGGCCCAGAC
    ACCTCTGGTC GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001504700.3
    CDS37..3651
    Translation

    Target ORF information:

    RefSeq Version XM_001504650.5
    Organism Equus caballus(horse)
    Definition Equus caballus nitric oxide synthase 3 (endothelial cell) (NOS3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001504650.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCTGGGCCAC CCTGCGGCCT GGGGCTGGGG 
    CTGGGCCTCG GGCTGTGTGG CAAGCAGGGC CCAGCCTCCC CCGCATCTGA GCCCAGCCGG
    GCACCTGTAC CCGCACCCGC ACCCGCACCC CTGCCTGCGC CAGACCACAG CCCCCCGCTC
    GCCAGGCCCC CAGAGGGGCC CAAGTTCCCG CGTGTGAAGA ACTGGGAGGT GGGGAGCATC
    ACCTACGACA CCCTGTGTGC CCAGTCCCAG CAGGATGGGC CCTGCACCCC AAGACGCTGC
    CTCGGCTCCC TGGTGTTTCC ACGGAAACTG CAGAGCCGGC CGTCCCAGGG CCCTCCGCCT
    CCTGAGCAGC TGCTGAGTCA GGCTAGGGAC TTCATCAACC AGTACTACAG CTCCATCAAG
    AGGAGCGGCT CCCAGGCCCA CGAGCAGCGT CTTCAGGAGG TGGAGGCCGA GGTGGCAGCC
    ACGGGCACCT ACCAGCTTCG GGAGAGTGAG CTGGTATTTG GGGCCAAGCA GGCCTGGCGC
    AACGCTCCCC GTTGTGTGGG CCGGATCCAG TGGGGGAAAC TGCAGGTCTT TGATGCGCGG
    GATTGCAGCT CTGCGCAGGA GATGTTCACC TGCATATGCA ACCACATCAA GTACGCCACC
    AACCGGGGCA ACCTTCGCTC TGCCATCACA GTGTTCCCCC AGCGCACCCC GGGCCGCGGA
    GACTTCAGAA TCTGGAACAG CCAACTGGTG CGCTATGCAG GCTACAGACA GCAGGACGGC
    TCTGTGCGGG GGGACCCAGC CAACGTGGAG ATCACCGAGC TCTGCGTCCA GCATGGCTGG
    ACTCCAGGAA ATGGCCGCTT TGATGTGCTG CCCCTGCTGC TCCAGGCCCC AGATGAACCC
    CCGGAACTCT TCACTCTGCC CCCCGAGCTG GTGCTTGAAG TGCCCCTGGA GCACCCCACG
    CTGGAGTGGT TCGCATCCCT GGGCCTGCGC TGGTACGCGC TCCCGGCAGT GTCCAACATG
    CTGCTGGAAA TCGGGGGCCT GGAGTTCCCT GCGGCCCCTT TCAGCGGCTG GTACATGAGC
    ACTGAGATCG GCACGCGGAA CCTGTGTGAC CCTCACCGCT ACAACATCCT GGAGGATGTG
    GCCGTCTGCA TGGACTTGGA TACCAGGACA ACCTCGTCCC TGTGGAAAGA CAAGGCAGCG
    GTGGAAATCA ACTTGGCTGT GCTGCACAGT TACCAGCTGG CCAAAGTGAC CATTGTGGAC
    CACCACGCCG CCACAGCCTC CTTCATGAAG CACCTGGAGA ATGAGCAGAA GGCGAGGGGG
    GGCTGCCCTG CCGACTGGGC CTGGATTGTG CCCCCCATCT CGGGCAGCCT CACCCCTGTC
    TTCCATCAGG AGATGGTCAA CTATGTCCTG TCCCCTGCCT TCCGCTACCA GCCAGACCCC
    TGGAAGGGGA GTGCAGTCAA GGGCACCGGC CTCGCCAGGA AGAAGACCTT TAAGGAAGTG
    GCCAATGCTG TGAAGATCTC TGCCTCCCTC ATGGGCACCG TGATGGCAAA GAGAGTGAAG
    GCTACAATCC TGTACGGCTC CGAGACCGGC CGGGCCCAGG GCTACGCACA GCAGCTGGGG
    AGGCTCTTCC GGAAGGCTTT CGACCCCCGG GTCCTGTGCA TGGATGAGTA TGATGTGGTG
    TCCCTCGAGC ACGAGACACT GGTGTTGGTG GTGACCAGCA CATTCGGGAA TGGGGATCCC
    CCAGAGAATG GAGAGAGTTT CGCAGCAGCC CTGATGGAGA TGTCGGGCCC TTACAACAGC
    TCCCCTCGGC CAGAACAGCA CAAGAGTTAC AAAATCCGCT TCAACAGCGT CTCCTGTTCA
    GACCCACTGG TGTCCTCCTG GCGGCGGAAG AGAAAGGAAT CCAGTAACAC AGACAGTGCA
    GGGGCACTGG GGACCCTCAG GTTCTGTGTG TTCGGGCTCG GCTCCCGGGC GTACCCGCAC
    TTCTGCGCCT TTGCTCGTGC GGTGGACACG CGGCTGGAAG AGCTGGGTGG CGAGCGGCTC
    CTGCAGCTGG GCCAGGGAGA CGAGCTGTGC GGCCAGGAGG AGGCCTTCCG TGGCTGGGCC
    CAAGCCGCCT TCCAGGCCTC CTGTGAGACC TTCTGCGTGG GAGAGGATGC CAAGGCTGCT
    GCCCGGGACA TATTCAGCCC CAAACGGAGC TGGAAGCGCC AGAGGTACCG GCTGAGCACC
    CAGGCTGAGG GCCTCCAGTT GCTACCAGGC CTGATCCATG TGCACCGGCG AAAGATGTTC
    CAGGCTACAG TCCTTTCAGT GGAAAATCTG CAAAGCAGCA GGTCCACCCG GGCCACGATC
    CTGGTGCGCC TGGACACTGG AGGCCAGGAG GGGCTCCAGT ACCAGCCAGG GGACCACATA
    GGCGTCTGCC CACCCAACCG GCCGGGACTC GTGGAGGCAC TGCTCAGCCG TGTGGAGGAC
    CCGCCGCCAG CAGGCGAGCC CGTGGCAGTG GAACAGCTGG AGAAGGGCAG CCCTGGTGGC
    CCTCCCCCAA GCTGGGTGCG GGACCCCCGG CTGCCCCCGT GCACGCTGCG CCAGGCTCTC
    ACCTTCTTCC TGGACATCAC TTCCCCGCCC AGCCCTCAAC TCCTTCGACT GCTCAGCACC
    TTGGCAGAAG AACCCAGTGA ACAGCAGGAG CTTGAGACCC TCAGCCAGGA CCCCCGGCGC
    TACGAAGAAT GGAAGTGGTT CCGCTGCCCC ACGCTGCTGG AGGTGCTGGA GCAGTTTCCT
    TCGGTGGCAC TGCCTGCTGC CCTGCTCCTC ACCCAGCTGC CCCTGCTCCA GCCCCGGTAC
    TACTCCGTCA GCTCAGCCCC CAGCACCCAC CCAGGAGAGA TCCACCTCAC AGTTGCCGTG
    CTGGCATACA GGACCCAGGA TGGGCTAGGC CCCCTGCACT ATGGAGTCTG CTCCACATGG
    CTGAGCCAAC TCAAGGCCGG AGACCTTGTA CCCTGCTTCA TCAGGGGGGC TCCCTCCTTC
    CGGCTGCCTC CGGACCCCAG CTTGCCCTGC ATCCTGGTGG GCCCCGGCAC GGGCATCGCC
    CCCTTCCGGG GATTTTGGCA GGAGCGGCTG CATGACATTG AGAGCAAAGG GCTGCAGCCC
    GCCCCCATGA CCTTGGTGTT CGGCTGCCGA TGCTCCCAGC TCGATCACCT CTACCGCGAC
    GAGGTGCAGG ATGCCCAGCA GCGCGGGGTA TTTGGCCGCG TCCTCACCGC CTTCTCCAGG
    GAACCGGACA GCCCTAAGAC CTACGTACAG GACATCCTGC GGACCGAGCT GGCTGCCGAG
    GTGCACCGCG TGCTGTGCCT CGATCGGGGC CACATGTTTG TCTGCGGTGA TGTCACCATG
    GCAACCAGCG TCTTGCAGAC GGTGCAACGC ATCCTGGCGA CAGAGGGCGA CATGGAACTG
    GACGAGGCCG GCGACGTCAT CGGCGTGCTG CGGGATCAGC AACGCTATCA CGAGGACATT
    TTCGGGCTCA CGCTGCGCAC CCAGGAGGTG ACAAGCCGCA TACGCACCCA GAGCCTTTCC
    TTGCACGAGC GGCATCTGCA GGGCACAGTG CCCTGGGCGT TCGACCCGCC CGGCCCAGAC
    ACCTCTGGTC GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.