chico cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	chico
Entrez Gene ID	64880
Full Name	CG5686 gene product from transcript CG5686-RB
Synonyms	BcDNA.GH11263,BcDNA:GH11263,CG5686,CHICO,Chico,Dmel\CG5686,IRS,IRS/chico,anon-WO0078940.1,anon-WO0078940.4,chic,dIRS,fs(2)4,fs(2)ry4
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 31B1-31B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_164899.3	NP_723540.1	chico, isoform A
NM_001201843.2	NP_001188772.1	chico, isoform B
NM_001273387.1	NP_001260316.1	chico, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study shows that the insulin receptor substrate gene chico controls the functioning of the systems of metabolism of dopamine and juvenile hormone in Drosophila melanogaster females under normal conditions and in thermal stress
Title: Mutation in the Drosophila insulin-like receptor substrate, chico, affects the neuroendocrine stress-reaction development.

Mutations in chico reduce expression of the rutabaga-type adenylyl cyclase (rut), leading to decreased cAMP synthesis in the mushroom bodies.
Title: Learning defects in Drosophila growth restricted chico mutants are caused by attenuated adenylyl cyclase activity.

longevity assurance mutants of chico, the Drosophila insulin receptor substrate homolog, require Drosophila d4eBP to slow aging.
Title: Drosophila Longevity Assurance Conferred by Reduced Insulin Receptor Substrate Chico Partially Requires d4eBP.

Expressing chico(+)in the mushroom bodies(MB) restores the number of MB neurons to wild-type amounts and also rescues chico learning defects.
Title: Mutations in the Drosophila insulin receptor substrate, CHICO, impair olfactory associative learning.

chico acts with the transcription factor FOXO to extend Drosophila lifespan
Title: Insulin receptor substrate chico acts with the transcription factor FOXO to extend Drosophila lifespan.

The following chico gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chico cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15353	NM_164899.3 Latest version!	Drosophila melanogaster chico, transcript variant A (chico), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa15353	NM_001201843.2 Latest version!	Drosophila melanogaster chico, transcript variant B (chico), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa15353	NM_001273387.1 Latest version!	Drosophila melanogaster chico, transcript variant C (chico), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chico ( NM_164899.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723540.1 Drosophila melanogaster chico, transcript variant A (chico), mRNA.

Price & Availability↑

CloneID	OFa15353
Clone ID Related Accession (Same CDS sequence)	NM_164899.3 , NM_001201843.2 , NM_001273387.1
Accession Version	NM_164899.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chico, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164899.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATCAA TATCGGATGA CGGCATGGCG CTGAGTGGCT ACCTCAAGAA GCTGAAGACC 
ATGAAGAAGA AGTTCTTTGT GCTGTACGAG GAGACGAGCA CTTCGGCAGC CCGGCTGGAG 
TACTACGATA CCGAAAAGAA GTTCCTGCAA AGAGCCGAGC CAAAAAGGGT TATATATCTG 
AAGAATTGCT TCAACATCAA TCGCCGTTTG GACACCAAGC ATAGATTTGT CATTGTGCTC 
TCCTCCAGAG ACGGTGGATT CGGCATCGTT CTCGAGAACG AAAATGATTT ACGCAAATGG 
TTGGACAAAC TACTAGTTCT ACAAAGGAAC ATAGCCAATT CGAATGGAAC AGCGCACTCA 
CCTTATGACC ACGTTTGGCA AGTTGTCATT CAAAAGAAGG GTATTTCGGA GAAAGTTGGA 
ATCACCGGAA CCTACCACTG TTGCCTTACT TCAAAATCCC TGACATTCGT GTGCATTGGA 
CCGGAGAAGA CGCCCAATGG CGAGGATCGC GTTGCGAGCA TTGAAATACT TTTGACCACG 
ATCAGGCGAT GCGGTCATGC ATCCCCACAA TGTATATTCT ACGTGGAACT TGGCCGCCAA 
AGTGTCTTGG GATCTGGTGA TCTGTGGATG GAGACGGATA ACGCAGCTAT TGCTACTAAT 
ATGCACAACA CGATACTGAG CGCTATGTCA GCCAAAACAG AGTCGAACAC GAATTTAATA 
AACGTTTATC AGAATAGACC TGACTTAAGT CACGAGCCCA TGAGAAAGCG ATCGTCGTCT 
GCAAACGAAG CATCGAAGCC GATAAACGTA AATGTCATAC AAAATAGTCA AAACTCTCTC 
GAATTGCGCA GCTGCAGTTC GCCCCATAAC TATGGTTTCG GCAGAGAGAG ATGCGATAGC 
TTACCAACCA GAAATGGAAC CCTAAGCGAG TCCAGCAATC AAACGTACTT TGGTTCCAAC 
CATGGACTGC GATCCAATAC TATATCTGGC ATCCGTCCGC ACTCAACCAA CAAGCATAGT 
AATAGTCCAA CGTTCACCAT GCCATTAAGA TGCTCAGAAT CCGAAGAGTC ATCAATTAGT 
GTCGATGAAT CCGACGACAA CGGCAGTTTT AGCCACTACA GATTAAACAC GCGGTCATCG 
GAGACGGCAA TTCCTGAGGA AAACATTGAT GACTTTGCCA GTGCGGAATT ATTTAGCAAA 
GTCACCGAAC AAAATGTAAG TGACGAAAAC TACATACCGA TGAATCCAGT CAATCCTACC 
GATGCTATCC ATGAAAAGGA GAAGGCTGAT ATGCAGAGAT TGGAAGATGC TTCGCTGCAT 
TTCAACTTTC CGGAGCACGC GTCGGAAAAG CTTGCTAAGG ATTTTGATCT GGACTCTGAT 
AACCAATGCT GTCGTCCCAT TCGCGCCTAT TCGATAGGCA ACAAGGTTGA GCATTTAAAG 
TTTAATAAGC GCCTGGGACA CTTGAATGAT ACGGGACAGA ATCCGAATCG CGTGCGAGCC 
TACTCGGTTG GCTCCAAATC GAAGATACCG CGCTGCGACC TGCAGCGAGT GGTCCTCGTG 
GAGGACAATA AACATGAGTT CACAGCGAAT AGGAGTCAGA GTAGCATTAC CAAGGAAGGA 
ACCAGCTATG GCAGCAGTGC CAATCGACAA AAGAAGTCCA CAAGTGCTCC ACTCCTCAGT 
CTGAAGAACC AGATAAACTC CGACCGAATG AGTGACTTAA TGGAAATTGA TTTTTCACAA 
GCAACCAATT TGGAAAAGCA GAAGTTCATC AAGAATAATG AAATTCCGAA ATACATTGAA 
AACGTGTTCC CAAAAGCCCC GCGAACGGAT AGCTCCAGCC TAACTCTGCA CGCCACAAGT 
CAAAAGGACA TTTTCAATGG CACCAAACTA AATAACACTG CGATCACATC CGAGGATGGT 
TACCTCGAGA TGAAGCCAGT CGGTAATGGA TACACTCCCA GTTCGAATTG CCTGCCAATG 
AAAGTGGAGA AACTCAAGCT ATCCGACTAT CAGACAGCAC CGCCACTCAC CGCAACAGCC 
GCACCAGTGC ACGATTTAAA CAAAATTAGC ACATACAATA TATCCGCTGA GAAATGGAGA 
GAACAGCCCA GCAGAAGCGA GGAAAAGAAG TCGAACTCGC CATTGAATGA CAACACCTTT 
AGCTCGAAAC CCACAAATGT CGAGAGTACA AGCAAAAGCC ATGATGTTCA TTCAGCAAAT 
CAAATTGATT GCGAGAAAGT GTGCGCGCAG AGCAGCGATA AGCTAAATAA TCATCTGGCC 
GACAAGATTG TCGAGAACAA CAATTTGGAT ATAGGCGGGC ATGAGGAAAA GAAGTTGGTT 
CATTCGATAA GCAGCGAAGA CTACACACAA ATCAAGGACA AATCGAATGA TTTCACAAAA 
TTTAACGAAG CCGGCTACAA AATTCTGCAA ATTAAAAGCG ACAGCTCACT CATCTCATCG 
AAGCTATACC AAAAGGGTAT ACACAAGGAT AACTTGGAGC GTTCGCAGAG ACTTACAGAG 
AGTGTGAATA CGATTCCCGA TAATGCCACC GCCACCGCGG TGAGCAGCAG CTCACTCACC 
AAATTCAATA TAAATTCAGC AAAGCCAGCC GCCGCCGCCG ATTCGCGTAG CACTGGCACA 
GATCCAAGTA CACCACAGAA CATTCTACAG ATTAAAGATT TGAATTTCCC CTCAAGGTCG 
TCGTCTCGCA TATCCCAGCC GGAGCTGCAC TACGCCAGCC TAGATCTTCC CCATTGCAGT 
GGCCAAAATC CAGCTAAATA CCTGAAGAGA GGATCACGCG AATCGCCGCC GGTGTCCGCA 
TGCCCGGAGG ATGGGAATAC CTATGCGAAA ATCGACTTTG ACCAATCCGA CTCCTCTTCC 
TCCTCATCGA ACATATTTAA TACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723540.1
CDS	198..3104
Translation	MASISDDGMALSGYLKKLKTMKKKFFVLYEETSTSAARLEYYDTEKKFLQRAEPKRVIYLKNCFNINRRLDTKHRFVIVLSSRDGGFGIVLENENDLRKWLDKLLVLQRNIANSNGTAHSPYDHVWQVVIQKKGISEKVGITGTYHCCLTSKSLTFVCIGPEKTPNGEDRVASIEILLTTIRRCGHASPQCIFYVELGRQSVLGSGDLWMETDNAAIATNMHNTILSAMSAKTESNTNLINVYQNRPDLSHEPMRKRSSSANEASKPINVNVIQNSQNSLELRSCSSPHNYGFGRERCDSLPTRNGTLSESSNQTYFGSNHGLRSNTISGIRPHSTNKHSNSPTFTMPLRCSESEESSISVDESDDNGSFSHYRLNTRSSETAIPEENIDDFASAELFSKVTEQNVSDENYIPMNPVNPTDAIHEKEKADMQRLEDASLHFNFPEHASEKLAKDFDLDSDNQCCRPIRAYSIGNKVEHLKFNKRLGHLNDTGQNPNRVRAYSVGSKSKIPRCDLQRVVLVEDNKHEFTANRSQSSITKEGTSYGSSANRQKKSTSAPLLSLKNQINSDRMSDLMEIDFSQATNLEKQKFIKNNEIPKYIENVFPKAPRTDSSSLTLHATSQKDIFNGTKLNNTAITSEDGYLEMKPVGNGYTPSSNCLPMKVEKLKLSDYQTAPPLTATAAPVHDLNKISTYNISAEKWREQPSRSEEKKSNSPLNDNTFSSKPTNVESTSKSHDVHSANQIDCEKVCAQSSDKLNNHLADKIVENNNLDIGGHEEKKLVHSISSEDYTQIKDKSNDFTKFNEAGYKILQIKSDSSLISSKLYQKGIHKDNLERSQRLTESVNTIPDNATATAVSSSSLTKFNINSAKPAAAADSRSTGTDPSTPQNILQIKDLNFPSRSSSRISQPELHYASLDLPHCSGQNPAKYLKRGSRESPPVSACPEDGNTYAKIDFDQSDSSSSSSNIFNT

Target ORF information:

RefSeq Version	NM_164899.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chico, transcript variant A (chico), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164899.3

ORF Insert Sequence:

ATGGCATCAA TATCGGATGA CGGCATGGCG CTGAGTGGCT ACCTCAAGAA GCTGAAGACC 
ATGAAGAAGA AGTTCTTTGT GCTGTACGAG GAGACGAGCA CTTCGGCAGC CCGGCTGGAG 
TACTACGATA CCGAAAAGAA GTTCCTGCAA AGAGCCGAGC CAAAAAGGGT TATATATCTG 
AAGAATTGCT TCAACATCAA TCGCCGTTTG GACACCAAGC ATAGATTTGT CATTGTGCTC 
TCCTCCAGAG ACGGTGGATT CGGCATCGTT CTCGAGAACG AAAATGATTT ACGCAAATGG 
TTGGACAAAC TACTAGTTCT ACAAAGGAAC ATAGCCAATT CGAATGGAAC AGCGCACTCA 
CCTTATGACC ACGTTTGGCA AGTTGTCATT CAAAAGAAGG GTATTTCGGA GAAAGTTGGA 
ATCACCGGAA CCTACCACTG TTGCCTTACT TCAAAATCCC TGACATTCGT GTGCATTGGA 
CCGGAGAAGA CGCCCAATGG CGAGGATCGC GTTGCGAGCA TTGAAATACT TTTGACCACG 
ATCAGGCGAT GCGGTCATGC ATCCCCACAA TGTATATTCT ACGTGGAACT TGGCCGCCAA 
AGTGTCTTGG GATCTGGTGA TCTGTGGATG GAGACGGATA ACGCAGCTAT TGCTACTAAT 
ATGCACAACA CGATACTGAG CGCTATGTCA GCCAAAACAG AGTCGAACAC GAATTTAATA 
AACGTTTATC AGAATAGACC TGACTTAAGT CACGAGCCCA TGAGAAAGCG ATCGTCGTCT 
GCAAACGAAG CATCGAAGCC GATAAACGTA AATGTCATAC AAAATAGTCA AAACTCTCTC 
GAATTGCGCA GCTGCAGTTC GCCCCATAAC TATGGTTTCG GCAGAGAGAG ATGCGATAGC 
TTACCAACCA GAAATGGAAC CCTAAGCGAG TCCAGCAATC AAACGTACTT TGGTTCCAAC 
CATGGACTGC GATCCAATAC TATATCTGGC ATCCGTCCGC ACTCAACCAA CAAGCATAGT 
AATAGTCCAA CGTTCACCAT GCCATTAAGA TGCTCAGAAT CCGAAGAGTC ATCAATTAGT 
GTCGATGAAT CCGACGACAA CGGCAGTTTT AGCCACTACA GATTAAACAC GCGGTCATCG 
GAGACGGCAA TTCCTGAGGA AAACATTGAT GACTTTGCCA GTGCGGAATT ATTTAGCAAA 
GTCACCGAAC AAAATGTAAG TGACGAAAAC TACATACCGA TGAATCCAGT CAATCCTACC 
GATGCTATCC ATGAAAAGGA GAAGGCTGAT ATGCAGAGAT TGGAAGATGC TTCGCTGCAT 
TTCAACTTTC CGGAGCACGC GTCGGAAAAG CTTGCTAAGG ATTTTGATCT GGACTCTGAT 
AACCAATGCT GTCGTCCCAT TCGCGCCTAT TCGATAGGCA ACAAGGTTGA GCATTTAAAG 
TTTAATAAGC GCCTGGGACA CTTGAATGAT ACGGGACAGA ATCCGAATCG CGTGCGAGCC 
TACTCGGTTG GCTCCAAATC GAAGATACCG CGCTGCGACC TGCAGCGAGT GGTCCTCGTG 
GAGGACAATA AACATGAGTT CACAGCGAAT AGGAGTCAGA GTAGCATTAC CAAGGAAGGA 
ACCAGCTATG GCAGCAGTGC CAATCGACAA AAGAAGTCCA CAAGTGCTCC ACTCCTCAGT 
CTGAAGAACC AGATAAACTC CGACCGAATG AGTGACTTAA TGGAAATTGA TTTTTCACAA 
GCAACCAATT TGGAAAAGCA GAAGTTCATC AAGAATAATG AAATTCCGAA ATACATTGAA 
AACGTGTTCC CAAAAGCCCC GCGAACGGAT AGCTCCAGCC TAACTCTGCA CGCCACAAGT 
CAAAAGGACA TTTTCAATGG CACCAAACTA AATAACACTG CGATCACATC CGAGGATGGT 
TACCTCGAGA TGAAGCCAGT CGGTAATGGA TACACTCCCA GTTCGAATTG CCTGCCAATG 
AAAGTGGAGA AACTCAAGCT ATCCGACTAT CAGACAGCAC CGCCACTCAC CGCAACAGCC 
GCACCAGTGC ACGATTTAAA CAAAATTAGC ACATACAATA TATCCGCTGA GAAATGGAGA 
GAACAGCCCA GCAGAAGCGA GGAAAAGAAG TCGAACTCGC CATTGAATGA CAACACCTTT 
AGCTCGAAAC CCACAAATGT CGAGAGTACA AGCAAAAGCC ATGATGTTCA TTCAGCAAAT 
CAAATTGATT GCGAGAAAGT GTGCGCGCAG AGCAGCGATA AGCTAAATAA TCATCTGGCC 
GACAAGATTG TCGAGAACAA CAATTTGGAT ATAGGCGGGC ATGAGGAAAA GAAGTTGGTT 
CATTCGATAA GCAGCGAAGA CTACACACAA ATCAAGGACA AATCGAATGA TTTCACAAAA 
TTTAACGAAG CCGGCTACAA AATTCTGCAA ATTAAAAGCG ACAGCTCACT CATCTCATCG 
AAGCTATACC AAAAGGGTAT ACACAAGGAT AACTTGGAGC GTTCGCAGAG ACTTACAGAG 
AGTGTGAATA CGATTCCCGA TAATGCCACC GCCACCGCGG TGAGCAGCAG CTCACTCACC 
AAATTCAATA TAAATTCAGC AAAGCCAGCC GCCGCCGCCG ATTCGCGTAG CACTGGCACA 
GATCCAAGTA CACCACAGAA CATTCTACAG ATTAAAGATT TGAATTTCCC CTCAAGGTCG 
TCGTCTCGCA TATCCCAGCC GGAGCTGCAC TACGCCAGCC TAGATCTTCC CCATTGCAGT 
GGCCAAAATC CAGCTAAATA CCTGAAGAGA GGATCACGCG AATCGCCGCC GGTGTCCGCA 
TGCCCGGAGG ATGGGAATAC CTATGCGAAA ATCGACTTTG ACCAATCCGA CTCCTCTTCC 
TCCTCATCGA ACATATTTAA TACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chico ( NM_001201843.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188772.1 Drosophila melanogaster chico, transcript variant B (chico), mRNA.

Price & Availability↑

CloneID	OFa15353
Clone ID Related Accession (Same CDS sequence)	NM_164899.3 , NM_001201843.2 , NM_001273387.1
Accession Version	NM_001201843.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chico, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001201843.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATCAA TATCGGATGA CGGCATGGCG CTGAGTGGCT ACCTCAAGAA GCTGAAGACC 
ATGAAGAAGA AGTTCTTTGT GCTGTACGAG GAGACGAGCA CTTCGGCAGC CCGGCTGGAG 
TACTACGATA CCGAAAAGAA GTTCCTGCAA AGAGCCGAGC CAAAAAGGGT TATATATCTG 
AAGAATTGCT TCAACATCAA TCGCCGTTTG GACACCAAGC ATAGATTTGT CATTGTGCTC 
TCCTCCAGAG ACGGTGGATT CGGCATCGTT CTCGAGAACG AAAATGATTT ACGCAAATGG 
TTGGACAAAC TACTAGTTCT ACAAAGGAAC ATAGCCAATT CGAATGGAAC AGCGCACTCA 
CCTTATGACC ACGTTTGGCA AGTTGTCATT CAAAAGAAGG GTATTTCGGA GAAAGTTGGA 
ATCACCGGAA CCTACCACTG TTGCCTTACT TCAAAATCCC TGACATTCGT GTGCATTGGA 
CCGGAGAAGA CGCCCAATGG CGAGGATCGC GTTGCGAGCA TTGAAATACT TTTGACCACG 
ATCAGGCGAT GCGGTCATGC ATCCCCACAA TGTATATTCT ACGTGGAACT TGGCCGCCAA 
AGTGTCTTGG GATCTGGTGA TCTGTGGATG GAGACGGATA ACGCAGCTAT TGCTACTAAT 
ATGCACAACA CGATACTGAG CGCTATGTCA GCCAAAACAG AGTCGAACAC GAATTTAATA 
AACGTTTATC AGAATAGACC TGACTTAAGT CACGAGCCCA TGAGAAAGCG ATCGTCGTCT 
GCAAACGAAG CATCGAAGCC GATAAACGTA AATGTCATAC AAAATAGTCA AAACTCTCTC 
GAATTGCGCA GCTGCAGTTC GCCCCATAAC TATGGTTTCG GCAGAGAGAG ATGCGATAGC 
TTACCAACCA GAAATGGAAC CCTAAGCGAG TCCAGCAATC AAACGTACTT TGGTTCCAAC 
CATGGACTGC GATCCAATAC TATATCTGGC ATCCGTCCGC ACTCAACCAA CAAGCATAGT 
AATAGTCCAA CGTTCACCAT GCCATTAAGA TGCTCAGAAT CCGAAGAGTC ATCAATTAGT 
GTCGATGAAT CCGACGACAA CGGCAGTTTT AGCCACTACA GATTAAACAC GCGGTCATCG 
GAGACGGCAA TTCCTGAGGA AAACATTGAT GACTTTGCCA GTGCGGAATT ATTTAGCAAA 
GTCACCGAAC AAAATGTAAG TGACGAAAAC TACATACCGA TGAATCCAGT CAATCCTACC 
GATGCTATCC ATGAAAAGGA GAAGGCTGAT ATGCAGAGAT TGGAAGATGC TTCGCTGCAT 
TTCAACTTTC CGGAGCACGC GTCGGAAAAG CTTGCTAAGG ATTTTGATCT GGACTCTGAT 
AACCAATGCT GTCGTCCCAT TCGCGCCTAT TCGATAGGCA ACAAGGTTGA GCATTTAAAG 
TTTAATAAGC GCCTGGGACA CTTGAATGAT ACGGGACAGA ATCCGAATCG CGTGCGAGCC 
TACTCGGTTG GCTCCAAATC GAAGATACCG CGCTGCGACC TGCAGCGAGT GGTCCTCGTG 
GAGGACAATA AACATGAGTT CACAGCGAAT AGGAGTCAGA GTAGCATTAC CAAGGAAGGA 
ACCAGCTATG GCAGCAGTGC CAATCGACAA AAGAAGTCCA CAAGTGCTCC ACTCCTCAGT 
CTGAAGAACC AGATAAACTC CGACCGAATG AGTGACTTAA TGGAAATTGA TTTTTCACAA 
GCAACCAATT TGGAAAAGCA GAAGTTCATC AAGAATAATG AAATTCCGAA ATACATTGAA 
AACGTGTTCC CAAAAGCCCC GCGAACGGAT AGCTCCAGCC TAACTCTGCA CGCCACAAGT 
CAAAAGGACA TTTTCAATGG CACCAAACTA AATAACACTG CGATCACATC CGAGGATGGT 
TACCTCGAGA TGAAGCCAGT CGGTAATGGA TACACTCCCA GTTCGAATTG CCTGCCAATG 
AAAGTGGAGA AACTCAAGCT ATCCGACTAT CAGACAGCAC CGCCACTCAC CGCAACAGCC 
GCACCAGTGC ACGATTTAAA CAAAATTAGC ACATACAATA TATCCGCTGA GAAATGGAGA 
GAACAGCCCA GCAGAAGCGA GGAAAAGAAG TCGAACTCGC CATTGAATGA CAACACCTTT 
AGCTCGAAAC CCACAAATGT CGAGAGTACA AGCAAAAGCC ATGATGTTCA TTCAGCAAAT 
CAAATTGATT GCGAGAAAGT GTGCGCGCAG AGCAGCGATA AGCTAAATAA TCATCTGGCC 
GACAAGATTG TCGAGAACAA CAATTTGGAT ATAGGCGGGC ATGAGGAAAA GAAGTTGGTT 
CATTCGATAA GCAGCGAAGA CTACACACAA ATCAAGGACA AATCGAATGA TTTCACAAAA 
TTTAACGAAG CCGGCTACAA AATTCTGCAA ATTAAAAGCG ACAGCTCACT CATCTCATCG 
AAGCTATACC AAAAGGGTAT ACACAAGGAT AACTTGGAGC GTTCGCAGAG ACTTACAGAG 
AGTGTGAATA CGATTCCCGA TAATGCCACC GCCACCGCGG TGAGCAGCAG CTCACTCACC 
AAATTCAATA TAAATTCAGC AAAGCCAGCC GCCGCCGCCG ATTCGCGTAG CACTGGCACA 
GATCCAAGTA CACCACAGAA CATTCTACAG ATTAAAGATT TGAATTTCCC CTCAAGGTCG 
TCGTCTCGCA TATCCCAGCC GGAGCTGCAC TACGCCAGCC TAGATCTTCC CCATTGCAGT 
GGCCAAAATC CAGCTAAATA CCTGAAGAGA GGATCACGCG AATCGCCGCC GGTGTCCGCA 
TGCCCGGAGG ATGGGAATAC CTATGCGAAA ATCGACTTTG ACCAATCCGA CTCCTCTTCC 
TCCTCATCGA ACATATTTAA TACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188772.1
CDS	73..2979
Translation	MASISDDGMALSGYLKKLKTMKKKFFVLYEETSTSAARLEYYDTEKKFLQRAEPKRVIYLKNCFNINRRLDTKHRFVIVLSSRDGGFGIVLENENDLRKWLDKLLVLQRNIANSNGTAHSPYDHVWQVVIQKKGISEKVGITGTYHCCLTSKSLTFVCIGPEKTPNGEDRVASIEILLTTIRRCGHASPQCIFYVELGRQSVLGSGDLWMETDNAAIATNMHNTILSAMSAKTESNTNLINVYQNRPDLSHEPMRKRSSSANEASKPINVNVIQNSQNSLELRSCSSPHNYGFGRERCDSLPTRNGTLSESSNQTYFGSNHGLRSNTISGIRPHSTNKHSNSPTFTMPLRCSESEESSISVDESDDNGSFSHYRLNTRSSETAIPEENIDDFASAELFSKVTEQNVSDENYIPMNPVNPTDAIHEKEKADMQRLEDASLHFNFPEHASEKLAKDFDLDSDNQCCRPIRAYSIGNKVEHLKFNKRLGHLNDTGQNPNRVRAYSVGSKSKIPRCDLQRVVLVEDNKHEFTANRSQSSITKEGTSYGSSANRQKKSTSAPLLSLKNQINSDRMSDLMEIDFSQATNLEKQKFIKNNEIPKYIENVFPKAPRTDSSSLTLHATSQKDIFNGTKLNNTAITSEDGYLEMKPVGNGYTPSSNCLPMKVEKLKLSDYQTAPPLTATAAPVHDLNKISTYNISAEKWREQPSRSEEKKSNSPLNDNTFSSKPTNVESTSKSHDVHSANQIDCEKVCAQSSDKLNNHLADKIVENNNLDIGGHEEKKLVHSISSEDYTQIKDKSNDFTKFNEAGYKILQIKSDSSLISSKLYQKGIHKDNLERSQRLTESVNTIPDNATATAVSSSSLTKFNINSAKPAAAADSRSTGTDPSTPQNILQIKDLNFPSRSSSRISQPELHYASLDLPHCSGQNPAKYLKRGSRESPPVSACPEDGNTYAKIDFDQSDSSSSSSNIFNT

Target ORF information:

RefSeq Version	NM_001201843.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chico, transcript variant B (chico), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201843.2

ORF Insert Sequence:

ATGGCATCAA TATCGGATGA CGGCATGGCG CTGAGTGGCT ACCTCAAGAA GCTGAAGACC 
ATGAAGAAGA AGTTCTTTGT GCTGTACGAG GAGACGAGCA CTTCGGCAGC CCGGCTGGAG 
TACTACGATA CCGAAAAGAA GTTCCTGCAA AGAGCCGAGC CAAAAAGGGT TATATATCTG 
AAGAATTGCT TCAACATCAA TCGCCGTTTG GACACCAAGC ATAGATTTGT CATTGTGCTC 
TCCTCCAGAG ACGGTGGATT CGGCATCGTT CTCGAGAACG AAAATGATTT ACGCAAATGG 
TTGGACAAAC TACTAGTTCT ACAAAGGAAC ATAGCCAATT CGAATGGAAC AGCGCACTCA 
CCTTATGACC ACGTTTGGCA AGTTGTCATT CAAAAGAAGG GTATTTCGGA GAAAGTTGGA 
ATCACCGGAA CCTACCACTG TTGCCTTACT TCAAAATCCC TGACATTCGT GTGCATTGGA 
CCGGAGAAGA CGCCCAATGG CGAGGATCGC GTTGCGAGCA TTGAAATACT TTTGACCACG 
ATCAGGCGAT GCGGTCATGC ATCCCCACAA TGTATATTCT ACGTGGAACT TGGCCGCCAA 
AGTGTCTTGG GATCTGGTGA TCTGTGGATG GAGACGGATA ACGCAGCTAT TGCTACTAAT 
ATGCACAACA CGATACTGAG CGCTATGTCA GCCAAAACAG AGTCGAACAC GAATTTAATA 
AACGTTTATC AGAATAGACC TGACTTAAGT CACGAGCCCA TGAGAAAGCG ATCGTCGTCT 
GCAAACGAAG CATCGAAGCC GATAAACGTA AATGTCATAC AAAATAGTCA AAACTCTCTC 
GAATTGCGCA GCTGCAGTTC GCCCCATAAC TATGGTTTCG GCAGAGAGAG ATGCGATAGC 
TTACCAACCA GAAATGGAAC CCTAAGCGAG TCCAGCAATC AAACGTACTT TGGTTCCAAC 
CATGGACTGC GATCCAATAC TATATCTGGC ATCCGTCCGC ACTCAACCAA CAAGCATAGT 
AATAGTCCAA CGTTCACCAT GCCATTAAGA TGCTCAGAAT CCGAAGAGTC ATCAATTAGT 
GTCGATGAAT CCGACGACAA CGGCAGTTTT AGCCACTACA GATTAAACAC GCGGTCATCG 
GAGACGGCAA TTCCTGAGGA AAACATTGAT GACTTTGCCA GTGCGGAATT ATTTAGCAAA 
GTCACCGAAC AAAATGTAAG TGACGAAAAC TACATACCGA TGAATCCAGT CAATCCTACC 
GATGCTATCC ATGAAAAGGA GAAGGCTGAT ATGCAGAGAT TGGAAGATGC TTCGCTGCAT 
TTCAACTTTC CGGAGCACGC GTCGGAAAAG CTTGCTAAGG ATTTTGATCT GGACTCTGAT 
AACCAATGCT GTCGTCCCAT TCGCGCCTAT TCGATAGGCA ACAAGGTTGA GCATTTAAAG 
TTTAATAAGC GCCTGGGACA CTTGAATGAT ACGGGACAGA ATCCGAATCG CGTGCGAGCC 
TACTCGGTTG GCTCCAAATC GAAGATACCG CGCTGCGACC TGCAGCGAGT GGTCCTCGTG 
GAGGACAATA AACATGAGTT CACAGCGAAT AGGAGTCAGA GTAGCATTAC CAAGGAAGGA 
ACCAGCTATG GCAGCAGTGC CAATCGACAA AAGAAGTCCA CAAGTGCTCC ACTCCTCAGT 
CTGAAGAACC AGATAAACTC CGACCGAATG AGTGACTTAA TGGAAATTGA TTTTTCACAA 
GCAACCAATT TGGAAAAGCA GAAGTTCATC AAGAATAATG AAATTCCGAA ATACATTGAA 
AACGTGTTCC CAAAAGCCCC GCGAACGGAT AGCTCCAGCC TAACTCTGCA CGCCACAAGT 
CAAAAGGACA TTTTCAATGG CACCAAACTA AATAACACTG CGATCACATC CGAGGATGGT 
TACCTCGAGA TGAAGCCAGT CGGTAATGGA TACACTCCCA GTTCGAATTG CCTGCCAATG 
AAAGTGGAGA AACTCAAGCT ATCCGACTAT CAGACAGCAC CGCCACTCAC CGCAACAGCC 
GCACCAGTGC ACGATTTAAA CAAAATTAGC ACATACAATA TATCCGCTGA GAAATGGAGA 
GAACAGCCCA GCAGAAGCGA GGAAAAGAAG TCGAACTCGC CATTGAATGA CAACACCTTT 
AGCTCGAAAC CCACAAATGT CGAGAGTACA AGCAAAAGCC ATGATGTTCA TTCAGCAAAT 
CAAATTGATT GCGAGAAAGT GTGCGCGCAG AGCAGCGATA AGCTAAATAA TCATCTGGCC 
GACAAGATTG TCGAGAACAA CAATTTGGAT ATAGGCGGGC ATGAGGAAAA GAAGTTGGTT 
CATTCGATAA GCAGCGAAGA CTACACACAA ATCAAGGACA AATCGAATGA TTTCACAAAA 
TTTAACGAAG CCGGCTACAA AATTCTGCAA ATTAAAAGCG ACAGCTCACT CATCTCATCG 
AAGCTATACC AAAAGGGTAT ACACAAGGAT AACTTGGAGC GTTCGCAGAG ACTTACAGAG 
AGTGTGAATA CGATTCCCGA TAATGCCACC GCCACCGCGG TGAGCAGCAG CTCACTCACC 
AAATTCAATA TAAATTCAGC AAAGCCAGCC GCCGCCGCCG ATTCGCGTAG CACTGGCACA 
GATCCAAGTA CACCACAGAA CATTCTACAG ATTAAAGATT TGAATTTCCC CTCAAGGTCG 
TCGTCTCGCA TATCCCAGCC GGAGCTGCAC TACGCCAGCC TAGATCTTCC CCATTGCAGT 
GGCCAAAATC CAGCTAAATA CCTGAAGAGA GGATCACGCG AATCGCCGCC GGTGTCCGCA 
TGCCCGGAGG ATGGGAATAC CTATGCGAAA ATCGACTTTG ACCAATCCGA CTCCTCTTCC 
TCCTCATCGA ACATATTTAA TACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chico ( NM_001273387.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260316.1 Drosophila melanogaster chico, transcript variant C (chico), mRNA.

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CloneID	OFa15353
Clone ID Related Accession (Same CDS sequence)	NM_164899.3 , NM_001201843.2 , NM_001273387.1
Accession Version	NM_001273387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chico, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATCAA TATCGGATGA CGGCATGGCG CTGAGTGGCT ACCTCAAGAA GCTGAAGACC 
ATGAAGAAGA AGTTCTTTGT GCTGTACGAG GAGACGAGCA CTTCGGCAGC CCGGCTGGAG 
TACTACGATA CCGAAAAGAA GTTCCTGCAA AGAGCCGAGC CAAAAAGGGT TATATATCTG 
AAGAATTGCT TCAACATCAA TCGCCGTTTG GACACCAAGC ATAGATTTGT CATTGTGCTC 
TCCTCCAGAG ACGGTGGATT CGGCATCGTT CTCGAGAACG AAAATGATTT ACGCAAATGG 
TTGGACAAAC TACTAGTTCT ACAAAGGAAC ATAGCCAATT CGAATGGAAC AGCGCACTCA 
CCTTATGACC ACGTTTGGCA AGTTGTCATT CAAAAGAAGG GTATTTCGGA GAAAGTTGGA 
ATCACCGGAA CCTACCACTG TTGCCTTACT TCAAAATCCC TGACATTCGT GTGCATTGGA 
CCGGAGAAGA CGCCCAATGG CGAGGATCGC GTTGCGAGCA TTGAAATACT TTTGACCACG 
ATCAGGCGAT GCGGTCATGC ATCCCCACAA TGTATATTCT ACGTGGAACT TGGCCGCCAA 
AGTGTCTTGG GATCTGGTGA TCTGTGGATG GAGACGGATA ACGCAGCTAT TGCTACTAAT 
ATGCACAACA CGATACTGAG CGCTATGTCA GCCAAAACAG AGTCGAACAC GAATTTAATA 
AACGTTTATC AGAATAGACC TGACTTAAGT CACGAGCCCA TGAGAAAGCG ATCGTCGTCT 
GCAAACGAAG CATCGAAGCC GATAAACGTA AATGTCATAC AAAATAGTCA AAACTCTCTC 
GAATTGCGCA GCTGCAGTTC GCCCCATAAC TATGGTTTCG GCAGAGAGAG ATGCGATAGC 
TTACCAACCA GAAATGGAAC CCTAAGCGAG TCCAGCAATC AAACGTACTT TGGTTCCAAC 
CATGGACTGC GATCCAATAC TATATCTGGC ATCCGTCCGC ACTCAACCAA CAAGCATAGT 
AATAGTCCAA CGTTCACCAT GCCATTAAGA TGCTCAGAAT CCGAAGAGTC ATCAATTAGT 
GTCGATGAAT CCGACGACAA CGGCAGTTTT AGCCACTACA GATTAAACAC GCGGTCATCG 
GAGACGGCAA TTCCTGAGGA AAACATTGAT GACTTTGCCA GTGCGGAATT ATTTAGCAAA 
GTCACCGAAC AAAATGTAAG TGACGAAAAC TACATACCGA TGAATCCAGT CAATCCTACC 
GATGCTATCC ATGAAAAGGA GAAGGCTGAT ATGCAGAGAT TGGAAGATGC TTCGCTGCAT 
TTCAACTTTC CGGAGCACGC GTCGGAAAAG CTTGCTAAGG ATTTTGATCT GGACTCTGAT 
AACCAATGCT GTCGTCCCAT TCGCGCCTAT TCGATAGGCA ACAAGGTTGA GCATTTAAAG 
TTTAATAAGC GCCTGGGACA CTTGAATGAT ACGGGACAGA ATCCGAATCG CGTGCGAGCC 
TACTCGGTTG GCTCCAAATC GAAGATACCG CGCTGCGACC TGCAGCGAGT GGTCCTCGTG 
GAGGACAATA AACATGAGTT CACAGCGAAT AGGAGTCAGA GTAGCATTAC CAAGGAAGGA 
ACCAGCTATG GCAGCAGTGC CAATCGACAA AAGAAGTCCA CAAGTGCTCC ACTCCTCAGT 
CTGAAGAACC AGATAAACTC CGACCGAATG AGTGACTTAA TGGAAATTGA TTTTTCACAA 
GCAACCAATT TGGAAAAGCA GAAGTTCATC AAGAATAATG AAATTCCGAA ATACATTGAA 
AACGTGTTCC CAAAAGCCCC GCGAACGGAT AGCTCCAGCC TAACTCTGCA CGCCACAAGT 
CAAAAGGACA TTTTCAATGG CACCAAACTA AATAACACTG CGATCACATC CGAGGATGGT 
TACCTCGAGA TGAAGCCAGT CGGTAATGGA TACACTCCCA GTTCGAATTG CCTGCCAATG 
AAAGTGGAGA AACTCAAGCT ATCCGACTAT CAGACAGCAC CGCCACTCAC CGCAACAGCC 
GCACCAGTGC ACGATTTAAA CAAAATTAGC ACATACAATA TATCCGCTGA GAAATGGAGA 
GAACAGCCCA GCAGAAGCGA GGAAAAGAAG TCGAACTCGC CATTGAATGA CAACACCTTT 
AGCTCGAAAC CCACAAATGT CGAGAGTACA AGCAAAAGCC ATGATGTTCA TTCAGCAAAT 
CAAATTGATT GCGAGAAAGT GTGCGCGCAG AGCAGCGATA AGCTAAATAA TCATCTGGCC 
GACAAGATTG TCGAGAACAA CAATTTGGAT ATAGGCGGGC ATGAGGAAAA GAAGTTGGTT 
CATTCGATAA GCAGCGAAGA CTACACACAA ATCAAGGACA AATCGAATGA TTTCACAAAA 
TTTAACGAAG CCGGCTACAA AATTCTGCAA ATTAAAAGCG ACAGCTCACT CATCTCATCG 
AAGCTATACC AAAAGGGTAT ACACAAGGAT AACTTGGAGC GTTCGCAGAG ACTTACAGAG 
AGTGTGAATA CGATTCCCGA TAATGCCACC GCCACCGCGG TGAGCAGCAG CTCACTCACC 
AAATTCAATA TAAATTCAGC AAAGCCAGCC GCCGCCGCCG ATTCGCGTAG CACTGGCACA 
GATCCAAGTA CACCACAGAA CATTCTACAG ATTAAAGATT TGAATTTCCC CTCAAGGTCG 
TCGTCTCGCA TATCCCAGCC GGAGCTGCAC TACGCCAGCC TAGATCTTCC CCATTGCAGT 
GGCCAAAATC CAGCTAAATA CCTGAAGAGA GGATCACGCG AATCGCCGCC GGTGTCCGCA 
TGCCCGGAGG ATGGGAATAC CTATGCGAAA ATCGACTTTG ACCAATCCGA CTCCTCTTCC 
TCCTCATCGA ACATATTTAA TACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260316.1
CDS	547..3453
Translation	MASISDDGMALSGYLKKLKTMKKKFFVLYEETSTSAARLEYYDTEKKFLQRAEPKRVIYLKNCFNINRRLDTKHRFVIVLSSRDGGFGIVLENENDLRKWLDKLLVLQRNIANSNGTAHSPYDHVWQVVIQKKGISEKVGITGTYHCCLTSKSLTFVCIGPEKTPNGEDRVASIEILLTTIRRCGHASPQCIFYVELGRQSVLGSGDLWMETDNAAIATNMHNTILSAMSAKTESNTNLINVYQNRPDLSHEPMRKRSSSANEASKPINVNVIQNSQNSLELRSCSSPHNYGFGRERCDSLPTRNGTLSESSNQTYFGSNHGLRSNTISGIRPHSTNKHSNSPTFTMPLRCSESEESSISVDESDDNGSFSHYRLNTRSSETAIPEENIDDFASAELFSKVTEQNVSDENYIPMNPVNPTDAIHEKEKADMQRLEDASLHFNFPEHASEKLAKDFDLDSDNQCCRPIRAYSIGNKVEHLKFNKRLGHLNDTGQNPNRVRAYSVGSKSKIPRCDLQRVVLVEDNKHEFTANRSQSSITKEGTSYGSSANRQKKSTSAPLLSLKNQINSDRMSDLMEIDFSQATNLEKQKFIKNNEIPKYIENVFPKAPRTDSSSLTLHATSQKDIFNGTKLNNTAITSEDGYLEMKPVGNGYTPSSNCLPMKVEKLKLSDYQTAPPLTATAAPVHDLNKISTYNISAEKWREQPSRSEEKKSNSPLNDNTFSSKPTNVESTSKSHDVHSANQIDCEKVCAQSSDKLNNHLADKIVENNNLDIGGHEEKKLVHSISSEDYTQIKDKSNDFTKFNEAGYKILQIKSDSSLISSKLYQKGIHKDNLERSQRLTESVNTIPDNATATAVSSSSLTKFNINSAKPAAAADSRSTGTDPSTPQNILQIKDLNFPSRSSSRISQPELHYASLDLPHCSGQNPAKYLKRGSRESPPVSACPEDGNTYAKIDFDQSDSSSSSSNIFNT

Target ORF information:

RefSeq Version	NM_001273387.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chico, transcript variant C (chico), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273387.1

ORF Insert Sequence:

ATGGCATCAA TATCGGATGA CGGCATGGCG CTGAGTGGCT ACCTCAAGAA GCTGAAGACC 
ATGAAGAAGA AGTTCTTTGT GCTGTACGAG GAGACGAGCA CTTCGGCAGC CCGGCTGGAG 
TACTACGATA CCGAAAAGAA GTTCCTGCAA AGAGCCGAGC CAAAAAGGGT TATATATCTG 
AAGAATTGCT TCAACATCAA TCGCCGTTTG GACACCAAGC ATAGATTTGT CATTGTGCTC 
TCCTCCAGAG ACGGTGGATT CGGCATCGTT CTCGAGAACG AAAATGATTT ACGCAAATGG 
TTGGACAAAC TACTAGTTCT ACAAAGGAAC ATAGCCAATT CGAATGGAAC AGCGCACTCA 
CCTTATGACC ACGTTTGGCA AGTTGTCATT CAAAAGAAGG GTATTTCGGA GAAAGTTGGA 
ATCACCGGAA CCTACCACTG TTGCCTTACT TCAAAATCCC TGACATTCGT GTGCATTGGA 
CCGGAGAAGA CGCCCAATGG CGAGGATCGC GTTGCGAGCA TTGAAATACT TTTGACCACG 
ATCAGGCGAT GCGGTCATGC ATCCCCACAA TGTATATTCT ACGTGGAACT TGGCCGCCAA 
AGTGTCTTGG GATCTGGTGA TCTGTGGATG GAGACGGATA ACGCAGCTAT TGCTACTAAT 
ATGCACAACA CGATACTGAG CGCTATGTCA GCCAAAACAG AGTCGAACAC GAATTTAATA 
AACGTTTATC AGAATAGACC TGACTTAAGT CACGAGCCCA TGAGAAAGCG ATCGTCGTCT 
GCAAACGAAG CATCGAAGCC GATAAACGTA AATGTCATAC AAAATAGTCA AAACTCTCTC 
GAATTGCGCA GCTGCAGTTC GCCCCATAAC TATGGTTTCG GCAGAGAGAG ATGCGATAGC 
TTACCAACCA GAAATGGAAC CCTAAGCGAG TCCAGCAATC AAACGTACTT TGGTTCCAAC 
CATGGACTGC GATCCAATAC TATATCTGGC ATCCGTCCGC ACTCAACCAA CAAGCATAGT 
AATAGTCCAA CGTTCACCAT GCCATTAAGA TGCTCAGAAT CCGAAGAGTC ATCAATTAGT 
GTCGATGAAT CCGACGACAA CGGCAGTTTT AGCCACTACA GATTAAACAC GCGGTCATCG 
GAGACGGCAA TTCCTGAGGA AAACATTGAT GACTTTGCCA GTGCGGAATT ATTTAGCAAA 
GTCACCGAAC AAAATGTAAG TGACGAAAAC TACATACCGA TGAATCCAGT CAATCCTACC 
GATGCTATCC ATGAAAAGGA GAAGGCTGAT ATGCAGAGAT TGGAAGATGC TTCGCTGCAT 
TTCAACTTTC CGGAGCACGC GTCGGAAAAG CTTGCTAAGG ATTTTGATCT GGACTCTGAT 
AACCAATGCT GTCGTCCCAT TCGCGCCTAT TCGATAGGCA ACAAGGTTGA GCATTTAAAG 
TTTAATAAGC GCCTGGGACA CTTGAATGAT ACGGGACAGA ATCCGAATCG CGTGCGAGCC 
TACTCGGTTG GCTCCAAATC GAAGATACCG CGCTGCGACC TGCAGCGAGT GGTCCTCGTG 
GAGGACAATA AACATGAGTT CACAGCGAAT AGGAGTCAGA GTAGCATTAC CAAGGAAGGA 
ACCAGCTATG GCAGCAGTGC CAATCGACAA AAGAAGTCCA CAAGTGCTCC ACTCCTCAGT 
CTGAAGAACC AGATAAACTC CGACCGAATG AGTGACTTAA TGGAAATTGA TTTTTCACAA 
GCAACCAATT TGGAAAAGCA GAAGTTCATC AAGAATAATG AAATTCCGAA ATACATTGAA 
AACGTGTTCC CAAAAGCCCC GCGAACGGAT AGCTCCAGCC TAACTCTGCA CGCCACAAGT 
CAAAAGGACA TTTTCAATGG CACCAAACTA AATAACACTG CGATCACATC CGAGGATGGT 
TACCTCGAGA TGAAGCCAGT CGGTAATGGA TACACTCCCA GTTCGAATTG CCTGCCAATG 
AAAGTGGAGA AACTCAAGCT ATCCGACTAT CAGACAGCAC CGCCACTCAC CGCAACAGCC 
GCACCAGTGC ACGATTTAAA CAAAATTAGC ACATACAATA TATCCGCTGA GAAATGGAGA 
GAACAGCCCA GCAGAAGCGA GGAAAAGAAG TCGAACTCGC CATTGAATGA CAACACCTTT 
AGCTCGAAAC CCACAAATGT CGAGAGTACA AGCAAAAGCC ATGATGTTCA TTCAGCAAAT 
CAAATTGATT GCGAGAAAGT GTGCGCGCAG AGCAGCGATA AGCTAAATAA TCATCTGGCC 
GACAAGATTG TCGAGAACAA CAATTTGGAT ATAGGCGGGC ATGAGGAAAA GAAGTTGGTT 
CATTCGATAA GCAGCGAAGA CTACACACAA ATCAAGGACA AATCGAATGA TTTCACAAAA 
TTTAACGAAG CCGGCTACAA AATTCTGCAA ATTAAAAGCG ACAGCTCACT CATCTCATCG 
AAGCTATACC AAAAGGGTAT ACACAAGGAT AACTTGGAGC GTTCGCAGAG ACTTACAGAG 
AGTGTGAATA CGATTCCCGA TAATGCCACC GCCACCGCGG TGAGCAGCAG CTCACTCACC 
AAATTCAATA TAAATTCAGC AAAGCCAGCC GCCGCCGCCG ATTCGCGTAG CACTGGCACA 
GATCCAAGTA CACCACAGAA CATTCTACAG ATTAAAGATT TGAATTTCCC CTCAAGGTCG 
TCGTCTCGCA TATCCCAGCC GGAGCTGCAC TACGCCAGCC TAGATCTTCC CCATTGCAGT 
GGCCAAAATC CAGCTAAATA CCTGAAGAGA GGATCACGCG AATCGCCGCC GGTGTCCGCA 
TGCCCGGAGG ATGGGAATAC CTATGCGAAA ATCGACTTTG ACCAATCCGA CTCCTCTTCC 
TCCTCATCGA ACATATTTAA TACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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chico cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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