MCU cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	MCU
Entrez Gene ID	59223
Full Name	CG18769 gene product from transcript CG18769-RH
Synonyms	CG8352,Dmel\CG18769,dMCU1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 65B4-65B5

mRNA and Protein(s)

mRNA	Protein	Name
NM_144449.4	NP_652706.3	mitochondrial calcium uniporter, isoform A
NM_168169.3	NP_729173.1	mitochondrial calcium uniporter, isoform B
NM_206282.2	NP_996004.1	mitochondrial calcium uniporter, isoform D
NM_001300025.1	NP_001286954.1	mitochondrial calcium uniporter, isoform I
NM_206280.2	NP_996002.2	mitochondrial calcium uniporter, isoform G
NM_001259718.1	NP_001246647.1	mitochondrial calcium uniporter, isoform H
NM_168170.2	NP_729174.2	mitochondrial calcium uniporter, isoform C
NM_206281.2	NP_996003.1	mitochondrial calcium uniporter, isoform E

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006330	XP_316356.3
Mus musculus (house mouse)	Mcu	NP_001028431.2
Gallus gallus (chicken)	MCU	XP_426497.2
Danio rerio (zebrafish)	mcu	NP_001070793.1
Drosophila melanogaster (fruit fly)	CG18769	NP_729173.1
Xenopus tropicalis (tropical clawed frog)	LOC101732332	XP_004920488.1
Homo sapiens (human)	MCU	NP_612366.1
Rattus norvegicus (Norway rat)	Mcu	NP_001099868.1
Pan troglodytes (chimpanzee)	MCU	XP_521691.2
Canis lupus familiaris (dog)	MCU	XP_546160.3
Bos taurus (cattle)	MCU	NP_001193031.1
Caenorhabditis elegans (roundworm)	mcu-1	NP_500892.1
Xenopus tropicalis (tropical clawed frog)	mcu	NP_001123699.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that MCU plays key role in endoplasmic reticulum-to-mitochondrial calcium transfer and for cell death due to mitochondrial dysfunction under oxidative stress.
Title: Mitochondrial calcium uniporter in

The following MCU gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MCU cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19482	NM_206280.2 Latest version!	Drosophila melanogaster mitochondrial calcium uniporter, transcript variant G (MCU), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa19483	NM_144449.4 Latest version!	Drosophila melanogaster mitochondrial calcium uniporter, transcript variant A (MCU), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa19483	NM_168170.2 Latest version!	Drosophila melanogaster mitochondrial calcium uniporter, transcript variant C (MCU), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa19483	NM_206282.2 Latest version!	Drosophila melanogaster mitochondrial calcium uniporter, transcript variant D (MCU), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa19483	NM_168169.3 Latest version!	Drosophila melanogaster mitochondrial calcium uniporter, transcript variant B (MCU), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa19484	NM_206281.2 Latest version!	Drosophila melanogaster mitochondrial calcium uniporter, transcript variant E (MCU), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa20414	NM_001259718.1 Latest version!	Drosophila melanogaster mitochondrial calcium uniporter, transcript variant H (MCU), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa22289	NM_001300025.1 Latest version!	Drosophila melanogaster mitochondrial calcium uniporter, transcript variant I (MCU), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MCU ( NM_206280.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996002.2 Drosophila melanogaster mitochondrial calcium uniporter, transcript variant G (MCU), mRNA.

Price & Availability↑

CloneID	OFa19482
Clone ID Related Accession (Same CDS sequence)	NM_206280.2
Accession Version	NM_206280.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial calcium uniporter, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_206280.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAGAA ATAGGGCTGC CATGGTCAGC GCCTTTCGGC TCTTCCTGCG ACCAGCGACC 
ACCACCACCC ATAGAAGTCT CGCCCTGCGT TTGGCCCCCG GCACCACCTT CGCCCTGCAC 
TTACGGCCAT GTCATGAGCT TCAGCAGCAC AGAAGCTTCG CATCCACGGC GGAGGATGGC 
GAAACGGACA AGCATAAAAA ACCGACGACG GGAGATATCA CGCGACTCAC TTTGGCCGAG 
CTGAGGACGC GGCTAAAAGC CCTCGGACTG AGTGCAGCGG GCCGAAAACA AGTCCTAGTT 
GAACGGCTAA CAAGATCTAC TACCTGCAGT GCCACAACGT TGCCAAGAAC AACAGGTTCC 
AATTTGTCGC CGGAGGTGTT CATTGGGGGG CTCGTAAATA ATTCCAACAC GACCTCTGAA 
CCCAAAAACA CTGTTCAACA AAATGAAGAC ATCTATGTGG AATACGTGAA TGGCATGCCA 
CACATGACTG TACGACTTCC CAGTCGCAAT GAACTCTGCC AGTTTGCCCT GAAACCCATC 
TCCCACAACG TTGGTGATCT GTTAGCCATG CTCAGGGCTG AGGATCGGGG CATCGATCGA 
GCTGCGGTGA TCAACAAGCA TGGTGTGCGA ATCGCGTCGT CCTGCACCAT CGAAAGCTTG 
CTGGATGACT CGTTTAGCAT ACAAATCAAC AATCGCACAT TGGATGTGAA TCCTCCCAAA 
CGTGACAAAG TCACATTGGA GTCCATGGAC AAAGTGGGCG ATGTTCGTAA GGTTATTGCT 
CAGCTCTACG AGGCATTCAA CGTCGGAGAA TATCAGCTGG AGAAGAGCAA TCAATTGGCC 
AAAGAACTGG AGACCCTACG CTATGAACTA GAACCACTGG AAGAGAAAAA ACTGGAACTC 
AGCAAAAAGG CCGCTCGTCG CACTAACTTT ATGACTTGGA TGGGTTTGGG CCTAATGTCG 
GTGCAATTTG GCATTTTGGC ACGACTGACT TGGTGGGAAT ACTCTTGGGA TATTATGGAA 
CCCGTTACAT ATTTTGTTAC TTACGGGACG ACAATGGCAA TGTATGCCTA CTATTGTGTC 
ACTAAGAGGG AATATATGAT GGAGGATGTG AAGAATCGCG AATTCTCACT TTCCCTTTAT 
AGGAACGCCA AAAAGGTTCA GTTCGATGTA GAACACTACA ACGAACTGAA GCGAAAGTCG 
GCGGAGTTGG AGTACAATCT CAGAAGGATC AACGACCCGC TCAACATGCA ATTGCCATCG 
CATTTGGTTC GGACCCAGGA AAACACGCCA CCAACCTTAA CTGAGGAAAA GGCGGAGCGG 
AAATACACTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996002.2
CDS	202..1533
Translation	MSRNRAAMVSAFRLFLRPATTTTHRSLALRLAPGTTFALHLRPCHELQQHRSFASTAEDGETDKHKKPTTGDITRLTLAELRTRLKALGLSAAGRKQVLVERLTRSTTCSATTLPRTTGSNLSPEVFIGGLVNNSNTTSEPKNTVQQNEDIYVEYVNGMPHMTVRLPSRNELCQFALKPISHNVGDLLAMLRAEDRGIDRAAVINKHGVRIASSCTIESLLDDSFSIQINNRTLDVNPPKRDKVTLESMDKVGDVRKVIAQLYEAFNVGEYQLEKSNQLAKELETLRYELEPLEEKKLELSKKAARRTNFMTWMGLGLMSVQFGILARLTWWEYSWDIMEPVTYFVTYGTTMAMYAYYCVTKREYMMEDVKNREFSLSLYRNAKKVQFDVEHYNELKRKSAELEYNLRRINDPLNMQLPSHLVRTQENTPPTLTEEKAERKYT

Target ORF information:

RefSeq Version	NM_206280.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial calcium uniporter, transcript variant G (MCU), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206280.2

ORF Insert Sequence:

ATGTCCAGAA ATAGGGCTGC CATGGTCAGC GCCTTTCGGC TCTTCCTGCG ACCAGCGACC 
ACCACCACCC ATAGAAGTCT CGCCCTGCGT TTGGCCCCCG GCACCACCTT CGCCCTGCAC 
TTACGGCCAT GTCATGAGCT TCAGCAGCAC AGAAGCTTCG CATCCACGGC GGAGGATGGC 
GAAACGGACA AGCATAAAAA ACCGACGACG GGAGATATCA CGCGACTCAC TTTGGCCGAG 
CTGAGGACGC GGCTAAAAGC CCTCGGACTG AGTGCAGCGG GCCGAAAACA AGTCCTAGTT 
GAACGGCTAA CAAGATCTAC TACCTGCAGT GCCACAACGT TGCCAAGAAC AACAGGTTCC 
AATTTGTCGC CGGAGGTGTT CATTGGGGGG CTCGTAAATA ATTCCAACAC GACCTCTGAA 
CCCAAAAACA CTGTTCAACA AAATGAAGAC ATCTATGTGG AATACGTGAA TGGCATGCCA 
CACATGACTG TACGACTTCC CAGTCGCAAT GAACTCTGCC AGTTTGCCCT GAAACCCATC 
TCCCACAACG TTGGTGATCT GTTAGCCATG CTCAGGGCTG AGGATCGGGG CATCGATCGA 
GCTGCGGTGA TCAACAAGCA TGGTGTGCGA ATCGCGTCGT CCTGCACCAT CGAAAGCTTG 
CTGGATGACT CGTTTAGCAT ACAAATCAAC AATCGCACAT TGGATGTGAA TCCTCCCAAA 
CGTGACAAAG TCACATTGGA GTCCATGGAC AAAGTGGGCG ATGTTCGTAA GGTTATTGCT 
CAGCTCTACG AGGCATTCAA CGTCGGAGAA TATCAGCTGG AGAAGAGCAA TCAATTGGCC 
AAAGAACTGG AGACCCTACG CTATGAACTA GAACCACTGG AAGAGAAAAA ACTGGAACTC 
AGCAAAAAGG CCGCTCGTCG CACTAACTTT ATGACTTGGA TGGGTTTGGG CCTAATGTCG 
GTGCAATTTG GCATTTTGGC ACGACTGACT TGGTGGGAAT ACTCTTGGGA TATTATGGAA 
CCCGTTACAT ATTTTGTTAC TTACGGGACG ACAATGGCAA TGTATGCCTA CTATTGTGTC 
ACTAAGAGGG AATATATGAT GGAGGATGTG AAGAATCGCG AATTCTCACT TTCCCTTTAT 
AGGAACGCCA AAAAGGTTCA GTTCGATGTA GAACACTACA ACGAACTGAA GCGAAAGTCG 
GCGGAGTTGG AGTACAATCT CAGAAGGATC AACGACCCGC TCAACATGCA ATTGCCATCG 
CATTTGGTTC GGACCCAGGA AAACACGCCA CCAACCTTAA CTGAGGAAAA GGCGGAGCGG 
AAATACACTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCU ( NM_144449.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_652706.3 Drosophila melanogaster mitochondrial calcium uniporter, transcript variant A (MCU), mRNA.

Price & Availability↑

CloneID	OFa19483
Clone ID Related Accession (Same CDS sequence)	NM_144449.4 , NM_168169.3 , NM_206282.2 , NM_168170.2
Accession Version	NM_144449.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial calcium uniporter, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_144449.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAGAA ATAGGGCTGC CATGGTCAGC GCCTTTCGGC TCTTCCTGCG ACCAGCGACC 
ACCACCACCC ATAGAAGTCT CGCCCTGCGT TTGGCCCCCG GCACCACCTT CGCCCTGCAC 
TTACGGCCAT GTCATGAGCT TCAGCAGCAC AGAAGCTTCG CATCCACGGC GGAGGATGGC 
GAAACGGACA AGCATAAAAA ACCGACGACG GGAGAAGACA TCTATGTGGA ATACGTGAAT 
GGCATGCCAC ACATGACTGT ACGACTTCCC AGTCGCAATG AACTCTGCCA GTTTGCCCTG 
AAACCCATCT CCCACAACGT TGGTGATCTG TTAGCCATGC TCAGGGCTGA GGATCGGGGC 
ATCGATCGAG CTGCGGTGAT CAACAAGCAT GGTGTGCGAA TCGCGTCGTC CTGCACCATC 
GAAAGCTTGC TGGATGACTC GTTTAGCATA CAAATCAACA ATCGCACATT GGATGTGAAT 
CCTCCCAAAC GTGACAAAGT CACATTGGAG TCCATGGACA AAGTGGGCGA TGTTCGTAAG 
GTTATTGCTC AGCTCTACGA GGCATTCAAC GTCGGAGAAT ATCAGCTGGA GAAGAGCAAT 
CAATTGGCCA AAGAACTGGA GACCCTACGC TATGAACTAG AACCACTGGA AGAGAAAAAA 
CTGGAACTCA GCAAAAAGGC CGCTCGTCGC ACTAACTTTA TGACTTGGAT GGGTTTGGGC 
CTAATGTCGG TGCAATTTGG CATTTTGGCA CGACTGACTT GGTGGGAATA CTCTTGGGAT 
ATTATGGAAC CCGTTACATA TTTTGTTACT TACGGGACGA CAATGGCAAT GTATGCCTAC 
TATTGTGTCA CTAAGAGGGA ATATATGATG GAGGATGTGA AGAATCGCGA ATTCTCACTT 
TCCCTTTATA GGAACGCCAA AAAGGTTCAG TTCGATGTAG AACACTACAA CGAACTGAAG 
CGAAAGTCGG CGGAGTTGGA GTACAATCTC AGAAGGATCA ACGACCCGCT CAACATGCAA 
TTGCCATCGC ATTTGGTTCG GACCCAGGAA AACACGCCAC CAACCTTAAC TGAGGAAAAG 
GCGGAGCGGA AATACACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_652706.3
CDS	168..1268
Translation	MSRNRAAMVSAFRLFLRPATTTTHRSLALRLAPGTTFALHLRPCHELQQHRSFASTAEDGETDKHKKPTTGEDIYVEYVNGMPHMTVRLPSRNELCQFALKPISHNVGDLLAMLRAEDRGIDRAAVINKHGVRIASSCTIESLLDDSFSIQINNRTLDVNPPKRDKVTLESMDKVGDVRKVIAQLYEAFNVGEYQLEKSNQLAKELETLRYELEPLEEKKLELSKKAARRTNFMTWMGLGLMSVQFGILARLTWWEYSWDIMEPVTYFVTYGTTMAMYAYYCVTKREYMMEDVKNREFSLSLYRNAKKVQFDVEHYNELKRKSAELEYNLRRINDPLNMQLPSHLVRTQENTPPTLTEEKAERKYT

Target ORF information:

RefSeq Version	NM_144449.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial calcium uniporter, transcript variant A (MCU), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144449.4

ORF Insert Sequence:

ATGTCCAGAA ATAGGGCTGC CATGGTCAGC GCCTTTCGGC TCTTCCTGCG ACCAGCGACC 
ACCACCACCC ATAGAAGTCT CGCCCTGCGT TTGGCCCCCG GCACCACCTT CGCCCTGCAC 
TTACGGCCAT GTCATGAGCT TCAGCAGCAC AGAAGCTTCG CATCCACGGC GGAGGATGGC 
GAAACGGACA AGCATAAAAA ACCGACGACG GGAGAAGACA TCTATGTGGA ATACGTGAAT 
GGCATGCCAC ACATGACTGT ACGACTTCCC AGTCGCAATG AACTCTGCCA GTTTGCCCTG 
AAACCCATCT CCCACAACGT TGGTGATCTG TTAGCCATGC TCAGGGCTGA GGATCGGGGC 
ATCGATCGAG CTGCGGTGAT CAACAAGCAT GGTGTGCGAA TCGCGTCGTC CTGCACCATC 
GAAAGCTTGC TGGATGACTC GTTTAGCATA CAAATCAACA ATCGCACATT GGATGTGAAT 
CCTCCCAAAC GTGACAAAGT CACATTGGAG TCCATGGACA AAGTGGGCGA TGTTCGTAAG 
GTTATTGCTC AGCTCTACGA GGCATTCAAC GTCGGAGAAT ATCAGCTGGA GAAGAGCAAT 
CAATTGGCCA AAGAACTGGA GACCCTACGC TATGAACTAG AACCACTGGA AGAGAAAAAA 
CTGGAACTCA GCAAAAAGGC CGCTCGTCGC ACTAACTTTA TGACTTGGAT GGGTTTGGGC 
CTAATGTCGG TGCAATTTGG CATTTTGGCA CGACTGACTT GGTGGGAATA CTCTTGGGAT 
ATTATGGAAC CCGTTACATA TTTTGTTACT TACGGGACGA CAATGGCAAT GTATGCCTAC 
TATTGTGTCA CTAAGAGGGA ATATATGATG GAGGATGTGA AGAATCGCGA ATTCTCACTT 
TCCCTTTATA GGAACGCCAA AAAGGTTCAG TTCGATGTAG AACACTACAA CGAACTGAAG 
CGAAAGTCGG CGGAGTTGGA GTACAATCTC AGAAGGATCA ACGACCCGCT CAACATGCAA 
TTGCCATCGC ATTTGGTTCG GACCCAGGAA AACACGCCAC CAACCTTAAC TGAGGAAAAG 
GCGGAGCGGA AATACACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCU ( NM_168169.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729173.1 Drosophila melanogaster mitochondrial calcium uniporter, transcript variant B (MCU), mRNA.

Price & Availability↑

CloneID	OFa19483
Clone ID Related Accession (Same CDS sequence)	NM_144449.4 , NM_168169.3 , NM_206282.2 , NM_168170.2
Accession Version	NM_168169.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial calcium uniporter, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_168169.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAGAA ATAGGGCTGC CATGGTCAGC GCCTTTCGGC TCTTCCTGCG ACCAGCGACC 
ACCACCACCC ATAGAAGTCT CGCCCTGCGT TTGGCCCCCG GCACCACCTT CGCCCTGCAC 
TTACGGCCAT GTCATGAGCT TCAGCAGCAC AGAAGCTTCG CATCCACGGC GGAGGATGGC 
GAAACGGACA AGCATAAAAA ACCGACGACG GGAGAAGACA TCTATGTGGA ATACGTGAAT 
GGCATGCCAC ACATGACTGT ACGACTTCCC AGTCGCAATG AACTCTGCCA GTTTGCCCTG 
AAACCCATCT CCCACAACGT TGGTGATCTG TTAGCCATGC TCAGGGCTGA GGATCGGGGC 
ATCGATCGAG CTGCGGTGAT CAACAAGCAT GGTGTGCGAA TCGCGTCGTC CTGCACCATC 
GAAAGCTTGC TGGATGACTC GTTTAGCATA CAAATCAACA ATCGCACATT GGATGTGAAT 
CCTCCCAAAC GTGACAAAGT CACATTGGAG TCCATGGACA AAGTGGGCGA TGTTCGTAAG 
GTTATTGCTC AGCTCTACGA GGCATTCAAC GTCGGAGAAT ATCAGCTGGA GAAGAGCAAT 
CAATTGGCCA AAGAACTGGA GACCCTACGC TATGAACTAG AACCACTGGA AGAGAAAAAA 
CTGGAACTCA GCAAAAAGGC CGCTCGTCGC ACTAACTTTA TGACTTGGAT GGGTTTGGGC 
CTAATGTCGG TGCAATTTGG CATTTTGGCA CGACTGACTT GGTGGGAATA CTCTTGGGAT 
ATTATGGAAC CCGTTACATA TTTTGTTACT TACGGGACGA CAATGGCAAT GTATGCCTAC 
TATTGTGTCA CTAAGAGGGA ATATATGATG GAGGATGTGA AGAATCGCGA ATTCTCACTT 
TCCCTTTATA GGAACGCCAA AAAGGTTCAG TTCGATGTAG AACACTACAA CGAACTGAAG 
CGAAAGTCGG CGGAGTTGGA GTACAATCTC AGAAGGATCA ACGACCCGCT CAACATGCAA 
TTGCCATCGC ATTTGGTTCG GACCCAGGAA AACACGCCAC CAACCTTAAC TGAGGAAAAG 
GCGGAGCGGA AATACACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729173.1
CDS	1095..2195
Translation	MSRNRAAMVSAFRLFLRPATTTTHRSLALRLAPGTTFALHLRPCHELQQHRSFASTAEDGETDKHKKPTTGEDIYVEYVNGMPHMTVRLPSRNELCQFALKPISHNVGDLLAMLRAEDRGIDRAAVINKHGVRIASSCTIESLLDDSFSIQINNRTLDVNPPKRDKVTLESMDKVGDVRKVIAQLYEAFNVGEYQLEKSNQLAKELETLRYELEPLEEKKLELSKKAARRTNFMTWMGLGLMSVQFGILARLTWWEYSWDIMEPVTYFVTYGTTMAMYAYYCVTKREYMMEDVKNREFSLSLYRNAKKVQFDVEHYNELKRKSAELEYNLRRINDPLNMQLPSHLVRTQENTPPTLTEEKAERKYT

Target ORF information:

RefSeq Version	NM_168169.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial calcium uniporter, transcript variant B (MCU), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168169.3

ORF Insert Sequence:

ATGTCCAGAA ATAGGGCTGC CATGGTCAGC GCCTTTCGGC TCTTCCTGCG ACCAGCGACC 
ACCACCACCC ATAGAAGTCT CGCCCTGCGT TTGGCCCCCG GCACCACCTT CGCCCTGCAC 
TTACGGCCAT GTCATGAGCT TCAGCAGCAC AGAAGCTTCG CATCCACGGC GGAGGATGGC 
GAAACGGACA AGCATAAAAA ACCGACGACG GGAGAAGACA TCTATGTGGA ATACGTGAAT 
GGCATGCCAC ACATGACTGT ACGACTTCCC AGTCGCAATG AACTCTGCCA GTTTGCCCTG 
AAACCCATCT CCCACAACGT TGGTGATCTG TTAGCCATGC TCAGGGCTGA GGATCGGGGC 
ATCGATCGAG CTGCGGTGAT CAACAAGCAT GGTGTGCGAA TCGCGTCGTC CTGCACCATC 
GAAAGCTTGC TGGATGACTC GTTTAGCATA CAAATCAACA ATCGCACATT GGATGTGAAT 
CCTCCCAAAC GTGACAAAGT CACATTGGAG TCCATGGACA AAGTGGGCGA TGTTCGTAAG 
GTTATTGCTC AGCTCTACGA GGCATTCAAC GTCGGAGAAT ATCAGCTGGA GAAGAGCAAT 
CAATTGGCCA AAGAACTGGA GACCCTACGC TATGAACTAG AACCACTGGA AGAGAAAAAA 
CTGGAACTCA GCAAAAAGGC CGCTCGTCGC ACTAACTTTA TGACTTGGAT GGGTTTGGGC 
CTAATGTCGG TGCAATTTGG CATTTTGGCA CGACTGACTT GGTGGGAATA CTCTTGGGAT 
ATTATGGAAC CCGTTACATA TTTTGTTACT TACGGGACGA CAATGGCAAT GTATGCCTAC 
TATTGTGTCA CTAAGAGGGA ATATATGATG GAGGATGTGA AGAATCGCGA ATTCTCACTT 
TCCCTTTATA GGAACGCCAA AAAGGTTCAG TTCGATGTAG AACACTACAA CGAACTGAAG 
CGAAAGTCGG CGGAGTTGGA GTACAATCTC AGAAGGATCA ACGACCCGCT CAACATGCAA 
TTGCCATCGC ATTTGGTTCG GACCCAGGAA AACACGCCAC CAACCTTAAC TGAGGAAAAG 
GCGGAGCGGA AATACACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCU ( NM_206282.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996004.1 Drosophila melanogaster mitochondrial calcium uniporter, transcript variant D (MCU), mRNA.

Price & Availability↑

CloneID	OFa19483
Clone ID Related Accession (Same CDS sequence)	NM_144449.4 , NM_168169.3 , NM_206282.2 , NM_168170.2
Accession Version	NM_206282.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial calcium uniporter, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_206282.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAGAA ATAGGGCTGC CATGGTCAGC GCCTTTCGGC TCTTCCTGCG ACCAGCGACC 
ACCACCACCC ATAGAAGTCT CGCCCTGCGT TTGGCCCCCG GCACCACCTT CGCCCTGCAC 
TTACGGCCAT GTCATGAGCT TCAGCAGCAC AGAAGCTTCG CATCCACGGC GGAGGATGGC 
GAAACGGACA AGCATAAAAA ACCGACGACG GGAGAAGACA TCTATGTGGA ATACGTGAAT 
GGCATGCCAC ACATGACTGT ACGACTTCCC AGTCGCAATG AACTCTGCCA GTTTGCCCTG 
AAACCCATCT CCCACAACGT TGGTGATCTG TTAGCCATGC TCAGGGCTGA GGATCGGGGC 
ATCGATCGAG CTGCGGTGAT CAACAAGCAT GGTGTGCGAA TCGCGTCGTC CTGCACCATC 
GAAAGCTTGC TGGATGACTC GTTTAGCATA CAAATCAACA ATCGCACATT GGATGTGAAT 
CCTCCCAAAC GTGACAAAGT CACATTGGAG TCCATGGACA AAGTGGGCGA TGTTCGTAAG 
GTTATTGCTC AGCTCTACGA GGCATTCAAC GTCGGAGAAT ATCAGCTGGA GAAGAGCAAT 
CAATTGGCCA AAGAACTGGA GACCCTACGC TATGAACTAG AACCACTGGA AGAGAAAAAA 
CTGGAACTCA GCAAAAAGGC CGCTCGTCGC ACTAACTTTA TGACTTGGAT GGGTTTGGGC 
CTAATGTCGG TGCAATTTGG CATTTTGGCA CGACTGACTT GGTGGGAATA CTCTTGGGAT 
ATTATGGAAC CCGTTACATA TTTTGTTACT TACGGGACGA CAATGGCAAT GTATGCCTAC 
TATTGTGTCA CTAAGAGGGA ATATATGATG GAGGATGTGA AGAATCGCGA ATTCTCACTT 
TCCCTTTATA GGAACGCCAA AAAGGTTCAG TTCGATGTAG AACACTACAA CGAACTGAAG 
CGAAAGTCGG CGGAGTTGGA GTACAATCTC AGAAGGATCA ACGACCCGCT CAACATGCAA 
TTGCCATCGC ATTTGGTTCG GACCCAGGAA AACACGCCAC CAACCTTAAC TGAGGAAAAG 
GCGGAGCGGA AATACACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996004.1
CDS	283..1383
Translation	MSRNRAAMVSAFRLFLRPATTTTHRSLALRLAPGTTFALHLRPCHELQQHRSFASTAEDGETDKHKKPTTGEDIYVEYVNGMPHMTVRLPSRNELCQFALKPISHNVGDLLAMLRAEDRGIDRAAVINKHGVRIASSCTIESLLDDSFSIQINNRTLDVNPPKRDKVTLESMDKVGDVRKVIAQLYEAFNVGEYQLEKSNQLAKELETLRYELEPLEEKKLELSKKAARRTNFMTWMGLGLMSVQFGILARLTWWEYSWDIMEPVTYFVTYGTTMAMYAYYCVTKREYMMEDVKNREFSLSLYRNAKKVQFDVEHYNELKRKSAELEYNLRRINDPLNMQLPSHLVRTQENTPPTLTEEKAERKYT

Target ORF information:

RefSeq Version	NM_206282.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial calcium uniporter, transcript variant D (MCU), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206282.2

ORF Insert Sequence:

ATGTCCAGAA ATAGGGCTGC CATGGTCAGC GCCTTTCGGC TCTTCCTGCG ACCAGCGACC 
ACCACCACCC ATAGAAGTCT CGCCCTGCGT TTGGCCCCCG GCACCACCTT CGCCCTGCAC 
TTACGGCCAT GTCATGAGCT TCAGCAGCAC AGAAGCTTCG CATCCACGGC GGAGGATGGC 
GAAACGGACA AGCATAAAAA ACCGACGACG GGAGAAGACA TCTATGTGGA ATACGTGAAT 
GGCATGCCAC ACATGACTGT ACGACTTCCC AGTCGCAATG AACTCTGCCA GTTTGCCCTG 
AAACCCATCT CCCACAACGT TGGTGATCTG TTAGCCATGC TCAGGGCTGA GGATCGGGGC 
ATCGATCGAG CTGCGGTGAT CAACAAGCAT GGTGTGCGAA TCGCGTCGTC CTGCACCATC 
GAAAGCTTGC TGGATGACTC GTTTAGCATA CAAATCAACA ATCGCACATT GGATGTGAAT 
CCTCCCAAAC GTGACAAAGT CACATTGGAG TCCATGGACA AAGTGGGCGA TGTTCGTAAG 
GTTATTGCTC AGCTCTACGA GGCATTCAAC GTCGGAGAAT ATCAGCTGGA GAAGAGCAAT 
CAATTGGCCA AAGAACTGGA GACCCTACGC TATGAACTAG AACCACTGGA AGAGAAAAAA 
CTGGAACTCA GCAAAAAGGC CGCTCGTCGC ACTAACTTTA TGACTTGGAT GGGTTTGGGC 
CTAATGTCGG TGCAATTTGG CATTTTGGCA CGACTGACTT GGTGGGAATA CTCTTGGGAT 
ATTATGGAAC CCGTTACATA TTTTGTTACT TACGGGACGA CAATGGCAAT GTATGCCTAC 
TATTGTGTCA CTAAGAGGGA ATATATGATG GAGGATGTGA AGAATCGCGA ATTCTCACTT 
TCCCTTTATA GGAACGCCAA AAAGGTTCAG TTCGATGTAG AACACTACAA CGAACTGAAG 
CGAAAGTCGG CGGAGTTGGA GTACAATCTC AGAAGGATCA ACGACCCGCT CAACATGCAA 
TTGCCATCGC ATTTGGTTCG GACCCAGGAA AACACGCCAC CAACCTTAAC TGAGGAAAAG 
GCGGAGCGGA AATACACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCU ( NM_168170.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729174.2 Drosophila melanogaster mitochondrial calcium uniporter, transcript variant C (MCU), mRNA.

Price & Availability↑

CloneID	OFa19483
Clone ID Related Accession (Same CDS sequence)	NM_144449.4 , NM_168169.3 , NM_206282.2 , NM_168170.2
Accession Version	NM_168170.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial calcium uniporter, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_168170.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAGAA ATAGGGCTGC CATGGTCAGC GCCTTTCGGC TCTTCCTGCG ACCAGCGACC 
ACCACCACCC ATAGAAGTCT CGCCCTGCGT TTGGCCCCCG GCACCACCTT CGCCCTGCAC 
TTACGGCCAT GTCATGAGCT TCAGCAGCAC AGAAGCTTCG CATCCACGGC GGAGGATGGC 
GAAACGGACA AGCATAAAAA ACCGACGACG GGAGAAGACA TCTATGTGGA ATACGTGAAT 
GGCATGCCAC ACATGACTGT ACGACTTCCC AGTCGCAATG AACTCTGCCA GTTTGCCCTG 
AAACCCATCT CCCACAACGT TGGTGATCTG TTAGCCATGC TCAGGGCTGA GGATCGGGGC 
ATCGATCGAG CTGCGGTGAT CAACAAGCAT GGTGTGCGAA TCGCGTCGTC CTGCACCATC 
GAAAGCTTGC TGGATGACTC GTTTAGCATA CAAATCAACA ATCGCACATT GGATGTGAAT 
CCTCCCAAAC GTGACAAAGT CACATTGGAG TCCATGGACA AAGTGGGCGA TGTTCGTAAG 
GTTATTGCTC AGCTCTACGA GGCATTCAAC GTCGGAGAAT ATCAGCTGGA GAAGAGCAAT 
CAATTGGCCA AAGAACTGGA GACCCTACGC TATGAACTAG AACCACTGGA AGAGAAAAAA 
CTGGAACTCA GCAAAAAGGC CGCTCGTCGC ACTAACTTTA TGACTTGGAT GGGTTTGGGC 
CTAATGTCGG TGCAATTTGG CATTTTGGCA CGACTGACTT GGTGGGAATA CTCTTGGGAT 
ATTATGGAAC CCGTTACATA TTTTGTTACT TACGGGACGA CAATGGCAAT GTATGCCTAC 
TATTGTGTCA CTAAGAGGGA ATATATGATG GAGGATGTGA AGAATCGCGA ATTCTCACTT 
TCCCTTTATA GGAACGCCAA AAAGGTTCAG TTCGATGTAG AACACTACAA CGAACTGAAG 
CGAAAGTCGG CGGAGTTGGA GTACAATCTC AGAAGGATCA ACGACCCGCT CAACATGCAA 
TTGCCATCGC ATTTGGTTCG GACCCAGGAA AACACGCCAC CAACCTTAAC TGAGGAAAAG 
GCGGAGCGGA AATACACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729174.2
CDS	250..1350
Translation	MSRNRAAMVSAFRLFLRPATTTTHRSLALRLAPGTTFALHLRPCHELQQHRSFASTAEDGETDKHKKPTTGEDIYVEYVNGMPHMTVRLPSRNELCQFALKPISHNVGDLLAMLRAEDRGIDRAAVINKHGVRIASSCTIESLLDDSFSIQINNRTLDVNPPKRDKVTLESMDKVGDVRKVIAQLYEAFNVGEYQLEKSNQLAKELETLRYELEPLEEKKLELSKKAARRTNFMTWMGLGLMSVQFGILARLTWWEYSWDIMEPVTYFVTYGTTMAMYAYYCVTKREYMMEDVKNREFSLSLYRNAKKVQFDVEHYNELKRKSAELEYNLRRINDPLNMQLPSHLVRTQENTPPTLTEEKAERKYT

Target ORF information:

RefSeq Version	NM_168170.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial calcium uniporter, transcript variant C (MCU), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168170.2

ORF Insert Sequence:

ATGTCCAGAA ATAGGGCTGC CATGGTCAGC GCCTTTCGGC TCTTCCTGCG ACCAGCGACC 
ACCACCACCC ATAGAAGTCT CGCCCTGCGT TTGGCCCCCG GCACCACCTT CGCCCTGCAC 
TTACGGCCAT GTCATGAGCT TCAGCAGCAC AGAAGCTTCG CATCCACGGC GGAGGATGGC 
GAAACGGACA AGCATAAAAA ACCGACGACG GGAGAAGACA TCTATGTGGA ATACGTGAAT 
GGCATGCCAC ACATGACTGT ACGACTTCCC AGTCGCAATG AACTCTGCCA GTTTGCCCTG 
AAACCCATCT CCCACAACGT TGGTGATCTG TTAGCCATGC TCAGGGCTGA GGATCGGGGC 
ATCGATCGAG CTGCGGTGAT CAACAAGCAT GGTGTGCGAA TCGCGTCGTC CTGCACCATC 
GAAAGCTTGC TGGATGACTC GTTTAGCATA CAAATCAACA ATCGCACATT GGATGTGAAT 
CCTCCCAAAC GTGACAAAGT CACATTGGAG TCCATGGACA AAGTGGGCGA TGTTCGTAAG 
GTTATTGCTC AGCTCTACGA GGCATTCAAC GTCGGAGAAT ATCAGCTGGA GAAGAGCAAT 
CAATTGGCCA AAGAACTGGA GACCCTACGC TATGAACTAG AACCACTGGA AGAGAAAAAA 
CTGGAACTCA GCAAAAAGGC CGCTCGTCGC ACTAACTTTA TGACTTGGAT GGGTTTGGGC 
CTAATGTCGG TGCAATTTGG CATTTTGGCA CGACTGACTT GGTGGGAATA CTCTTGGGAT 
ATTATGGAAC CCGTTACATA TTTTGTTACT TACGGGACGA CAATGGCAAT GTATGCCTAC 
TATTGTGTCA CTAAGAGGGA ATATATGATG GAGGATGTGA AGAATCGCGA ATTCTCACTT 
TCCCTTTATA GGAACGCCAA AAAGGTTCAG TTCGATGTAG AACACTACAA CGAACTGAAG 
CGAAAGTCGG CGGAGTTGGA GTACAATCTC AGAAGGATCA ACGACCCGCT CAACATGCAA 
TTGCCATCGC ATTTGGTTCG GACCCAGGAA AACACGCCAC CAACCTTAAC TGAGGAAAAG 
GCGGAGCGGA AATACACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCU ( NM_206281.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996003.1 Drosophila melanogaster mitochondrial calcium uniporter, transcript variant E (MCU), mRNA.

Price & Availability↑

CloneID	OFa19484
Clone ID Related Accession (Same CDS sequence)	NM_206281.2
Accession Version	NM_206281.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial calcium uniporter, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_206281.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCACACA TGACTGTACG ACTTCCCAGT CGCAATGAAC TCTGCCAGTT TGCCCTGAAA 
CCCATCTCCC ACAACGTTGG TGATCTGTTA GCCATGCTCA GGGCTGAGGA TCGGGGCATC 
GATCGAGCTG CGGTGATCAA CAAGCATGGT GTGCGAATCG CGTCGTCCTG CACCATCGAA 
AGCTTGCTGG ATGACTCGTT TAGCATACAA ATCAACAATC GCACATTGGA TGTGAATCCT 
CCCAAACGTG ACAAAGTCAC ATTGGAGTCC ATGGACAAAG TGGGCGATGT TCGTAAGGTT 
ATTGCTCAGC TCTACGAGGC ATTCAACGTC GGAGAATATC AGCTGGAGAA GAGCAATCAA 
TTGGCCAAAG AACTGGAGAC CCTACGCTAT GAACTAGAAC CACTGGAAGA GAAAAAACTG 
GAACTCAGCA AAAAGGCCGC TCGTCGCACT AACTTTATGA CTTGGATGGG TTTGGGCCTA 
ATGTCGGTGC AATTTGGCAT TTTGGCACGA CTGACTTGGT GGGAATACTC TTGGGATATT 
ATGGAACCCG TTACATATTT TGTTACTTAC GGGACGACAA TGGCAATGTA TGCCTACTAT 
TGTGTCACTA AGAGGGAATA TATGATGGAG GATGTGAAGA ATCGCGAATT CTCACTTTCC 
CTTTATAGGA ACGCCAAAAA GGTTCAGTTC GATGTAGAAC ACTACAACGA ACTGAAGCGA 
AAGTCGGCGG AGTTGGAGTA CAATCTCAGA AGGATCAACG ACCCGCTCAA CATGCAATTG 
CCATCGCATT TGGTTCGGAC CCAGGAAAAC ACGCCACCAA CCTTAACTGA GGAAAAGGCG 
GAGCGGAAAT ACACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996003.1
CDS	258..1115
Translation	MPHMTVRLPSRNELCQFALKPISHNVGDLLAMLRAEDRGIDRAAVINKHGVRIASSCTIESLLDDSFSIQINNRTLDVNPPKRDKVTLESMDKVGDVRKVIAQLYEAFNVGEYQLEKSNQLAKELETLRYELEPLEEKKLELSKKAARRTNFMTWMGLGLMSVQFGILARLTWWEYSWDIMEPVTYFVTYGTTMAMYAYYCVTKREYMMEDVKNREFSLSLYRNAKKVQFDVEHYNELKRKSAELEYNLRRINDPLNMQLPSHLVRTQENTPPTLTEEKAERKYT

Target ORF information:

RefSeq Version	NM_206281.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial calcium uniporter, transcript variant E (MCU), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206281.2

ORF Insert Sequence:

ATGCCACACA TGACTGTACG ACTTCCCAGT CGCAATGAAC TCTGCCAGTT TGCCCTGAAA 
CCCATCTCCC ACAACGTTGG TGATCTGTTA GCCATGCTCA GGGCTGAGGA TCGGGGCATC 
GATCGAGCTG CGGTGATCAA CAAGCATGGT GTGCGAATCG CGTCGTCCTG CACCATCGAA 
AGCTTGCTGG ATGACTCGTT TAGCATACAA ATCAACAATC GCACATTGGA TGTGAATCCT 
CCCAAACGTG ACAAAGTCAC ATTGGAGTCC ATGGACAAAG TGGGCGATGT TCGTAAGGTT 
ATTGCTCAGC TCTACGAGGC ATTCAACGTC GGAGAATATC AGCTGGAGAA GAGCAATCAA 
TTGGCCAAAG AACTGGAGAC CCTACGCTAT GAACTAGAAC CACTGGAAGA GAAAAAACTG 
GAACTCAGCA AAAAGGCCGC TCGTCGCACT AACTTTATGA CTTGGATGGG TTTGGGCCTA 
ATGTCGGTGC AATTTGGCAT TTTGGCACGA CTGACTTGGT GGGAATACTC TTGGGATATT 
ATGGAACCCG TTACATATTT TGTTACTTAC GGGACGACAA TGGCAATGTA TGCCTACTAT 
TGTGTCACTA AGAGGGAATA TATGATGGAG GATGTGAAGA ATCGCGAATT CTCACTTTCC 
CTTTATAGGA ACGCCAAAAA GGTTCAGTTC GATGTAGAAC ACTACAACGA ACTGAAGCGA 
AAGTCGGCGG AGTTGGAGTA CAATCTCAGA AGGATCAACG ACCCGCTCAA CATGCAATTG 
CCATCGCATT TGGTTCGGAC CCAGGAAAAC ACGCCACCAA CCTTAACTGA GGAAAAGGCG 
GAGCGGAAAT ACACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCU ( NM_001259718.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246647.1 Drosophila melanogaster mitochondrial calcium uniporter, transcript variant H (MCU), mRNA.

Price & Availability↑

CloneID	OFa20414
Clone ID Related Accession (Same CDS sequence)	NM_001259718.1
Accession Version	NM_001259718.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial calcium uniporter, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAGAA ATAGGGCTGC CATGGTCAGC GCCTTTCGGC TCTTCCTGCG ACCAGCGACC 
ACCACCACCC ATAGAAGTCT CGCCCTGCGT TTGGCCCCCG GCACCACCTT CGCCCTGCAC 
TTACGGCCAT GTCATGAGCT TCAGCAGCAC AGAAGCTTCG CATCCACGGC GGAGGATGGC 
GAAACGGACA AGCATAAAAA ACCGACGACG GGAGGTCACA GTTCAAGTCA GCATGTGGAT 
GAGCAAGCAG GATGTGATGA TAAAATAAGA GAAAAGAAAT CGAAATCCAA TCACTGTTTA 
GTTAAGTATC GAAAAGGGGA TCAGGAAACT GAAAAAGGTA CCAATCCCTG CGGAGCTCAA 
GAATTTAAAG AATCAAATTC GTTTGCTGGC ACTCAAGTTC ATTCACTCAA GAAGAGAATC 
AAGAATAGGA AAATAAAGAA GAAATTGATA CTAAATAGAA AGAAAAAGAA AAAGAAAACT 
TGTAATGAAG ACATCTATGT GGAATACGTG AATGGCATGC CACACATGAC TGTACGACTT 
CCCAGTCGCA ATGAACTCTG CCAGTTTGCC CTGAAACCCA TCTCCCACAA CGTTGGTGAT 
CTGTTAGCCA TGCTCAGGGC TGAGGATCGG GGCATCGATC GAGCTGCGGT GATCAACAAG 
CATGGTGTGC GAATCGCGTC GTCCTGCACC ATCGAAAGCT TGCTGGATGA CTCGTTTAGC 
ATACAAATCA ACAATCGCAC ATTGGATGTG AATCCTCCCA AACGTGACAA AGTCACATTG 
GAGTCCATGG ACAAAGTGGG CGATGTTCGT AAGGTTATTG CTCAGCTCTA CGAGGCATTC 
AACGTCGGAG AATATCAGCT GGAGAAGAGC AATCAATTGG CCAAAGAACT GGAGACCCTA 
CGCTATGAAC TAGAACCACT GGAAGAGAAA AAACTGGAAC TCAGCAAAAA GGCCGCTCGT 
CGCACTAACT TTATGACTTG GATGGGTTTG GGCCTAATGT CGGTGCAATT TGGCATTTTG 
GCACGACTGA CTTGGTGGGA ATACTCTTGG GATATTATGG AACCCGTTAC ATATTTTGTT 
ACTTACGGGA CGACAATGGC AATGTATGCC TACTATTGTG TCACTAAGAG GGAATATATG 
ATGGAGGATG TGAAGAATCG CGAATTCTCA CTTTCCCTTT ATAGGAACGC CAAAAAGGTT 
CAGTTCGATG TAGAACACTA CAACGAACTG AAGCGAAAGT CGGCGGAGTT GGAGTACAAT 
CTCAGAAGGA TCAACGACCC GCTCAACATG CAATTGCCAT CGCATTTGGT TCGGACCCAG 
GAAAACACGC CACCAACCTT AACTGAGGAA AAGGCGGAGC GGAAATACAC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246647.1
CDS	202..1575
Translation	MSRNRAAMVSAFRLFLRPATTTTHRSLALRLAPGTTFALHLRPCHELQQHRSFASTAEDGETDKHKKPTTGGHSSSQHVDEQAGCDDKIREKKSKSNHCLVKYRKGDQETEKGTNPCGAQEFKESNSFAGTQVHSLKKRIKNRKIKKKLILNRKKKKKKTCNEDIYVEYVNGMPHMTVRLPSRNELCQFALKPISHNVGDLLAMLRAEDRGIDRAAVINKHGVRIASSCTIESLLDDSFSIQINNRTLDVNPPKRDKVTLESMDKVGDVRKVIAQLYEAFNVGEYQLEKSNQLAKELETLRYELEPLEEKKLELSKKAARRTNFMTWMGLGLMSVQFGILARLTWWEYSWDIMEPVTYFVTYGTTMAMYAYYCVTKREYMMEDVKNREFSLSLYRNAKKVQFDVEHYNELKRKSAELEYNLRRINDPLNMQLPSHLVRTQENTPPTLTEEKAERKYT

Target ORF information:

RefSeq Version	NM_001259718.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial calcium uniporter, transcript variant H (MCU), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259718.1

ORF Insert Sequence:

ATGTCCAGAA ATAGGGCTGC CATGGTCAGC GCCTTTCGGC TCTTCCTGCG ACCAGCGACC 
ACCACCACCC ATAGAAGTCT CGCCCTGCGT TTGGCCCCCG GCACCACCTT CGCCCTGCAC 
TTACGGCCAT GTCATGAGCT TCAGCAGCAC AGAAGCTTCG CATCCACGGC GGAGGATGGC 
GAAACGGACA AGCATAAAAA ACCGACGACG GGAGGTCACA GTTCAAGTCA GCATGTGGAT 
GAGCAAGCAG GATGTGATGA TAAAATAAGA GAAAAGAAAT CGAAATCCAA TCACTGTTTA 
GTTAAGTATC GAAAAGGGGA TCAGGAAACT GAAAAAGGTA CCAATCCCTG CGGAGCTCAA 
GAATTTAAAG AATCAAATTC GTTTGCTGGC ACTCAAGTTC ATTCACTCAA GAAGAGAATC 
AAGAATAGGA AAATAAAGAA GAAATTGATA CTAAATAGAA AGAAAAAGAA AAAGAAAACT 
TGTAATGAAG ACATCTATGT GGAATACGTG AATGGCATGC CACACATGAC TGTACGACTT 
CCCAGTCGCA ATGAACTCTG CCAGTTTGCC CTGAAACCCA TCTCCCACAA CGTTGGTGAT 
CTGTTAGCCA TGCTCAGGGC TGAGGATCGG GGCATCGATC GAGCTGCGGT GATCAACAAG 
CATGGTGTGC GAATCGCGTC GTCCTGCACC ATCGAAAGCT TGCTGGATGA CTCGTTTAGC 
ATACAAATCA ACAATCGCAC ATTGGATGTG AATCCTCCCA AACGTGACAA AGTCACATTG 
GAGTCCATGG ACAAAGTGGG CGATGTTCGT AAGGTTATTG CTCAGCTCTA CGAGGCATTC 
AACGTCGGAG AATATCAGCT GGAGAAGAGC AATCAATTGG CCAAAGAACT GGAGACCCTA 
CGCTATGAAC TAGAACCACT GGAAGAGAAA AAACTGGAAC TCAGCAAAAA GGCCGCTCGT 
CGCACTAACT TTATGACTTG GATGGGTTTG GGCCTAATGT CGGTGCAATT TGGCATTTTG 
GCACGACTGA CTTGGTGGGA ATACTCTTGG GATATTATGG AACCCGTTAC ATATTTTGTT 
ACTTACGGGA CGACAATGGC AATGTATGCC TACTATTGTG TCACTAAGAG GGAATATATG 
ATGGAGGATG TGAAGAATCG CGAATTCTCA CTTTCCCTTT ATAGGAACGC CAAAAAGGTT 
CAGTTCGATG TAGAACACTA CAACGAACTG AAGCGAAAGT CGGCGGAGTT GGAGTACAAT 
CTCAGAAGGA TCAACGACCC GCTCAACATG CAATTGCCAT CGCATTTGGT TCGGACCCAG 
GAAAACACGC CACCAACCTT AACTGAGGAA AAGGCGGAGC GGAAATACAC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCU ( NM_001300025.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286954.1 Drosophila melanogaster mitochondrial calcium uniporter, transcript variant I (MCU), mRNA.

Price & Availability↑

CloneID	OFa22289
Clone ID Related Accession (Same CDS sequence)	NM_001300025.1
Accession Version	NM_001300025.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial calcium uniporter, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAGAA ATAGGGCTGC CATGGTCAGC GCCTTTCGGC TCTTCCTGCG ACCAGCGACC 
ACCACCACCC ATAGAAGTCT CGCCCTGCGT TTGGCCCCCG GCACCACCTT CGCCCTGCAC 
TTACGGCCAT GTCATGAGCT TCAGCAGCAC AGAAGCTTCG CATCCACGGC GGAGGATGGC 
GAAACGGACA AGCATAAAAA ACCGACGACG GGAGACATCT ATGTGGAATA CGTGAATGGC 
ATGCCACACA TGACTGTACG ACTTCCCAGT CGCAATGAAC TCTGCCAGTT TGCCCTGAAA 
CCCATCTCCC ACAACGTTGG TGATCTGTTA GCCATGCTCA GGGCTGAGGA TCGGGGCATC 
GATCGAGCTG CGGTGATCAA CAAGCATGGT GTGCGAATCG CGTCGTCCTG CACCATCGAA 
AGCTTGCTGG ATGACTCGTT TAGCATACAA ATCAACAATC GCACATTGGA TGTGAATCCT 
CCCAAACGTG ACAAAGTCAC ATTGGAGTCC ATGGACAAAG TGGGCGATGT TCGTAAGGTT 
ATTGCTCAGC TCTACGAGGC ATTCAACGTC GGAGAATATC AGCTGGAGAA GAGCAATCAA 
TTGGCCAAAG AACTGGAGAC CCTACGCTAT GAACTAGAAC CACTGGAAGA GAAAAAACTG 
GAACTCAGCA AAAAGGCCGC TCGTCGCACT AACTTTATGA CTTGGATGGG TTTGGGCCTA 
ATGTCGGTGC AATTTGGCAT TTTGGCACGA CTGACTTGGT GGGAATACTC TTGGGATATT 
ATGGAACCCG TTACATATTT TGTTACTTAC GGGACGACAA TGGCAATGTA TGCCTACTAT 
TGTGTCACTA AGAGGGAATA TATGATGGAG GATGTGAAGA ATCGCGAATT CTCACTTTCC 
CTTTATAGGA ACGCCAAAAA GGTTCAGTTC GATGTAGAAC ACTACAACGA ACTGAAGCGA 
AAGTCGGCGG AGTTGGAGTA CAATCTCAGA AGGATCAACG ACCCGCTCAA CATGCAATTG 
CCATCGCATT TGGTTCGGAC CCAGGAAAAC ACGCCACCAA CCTTAACTGA GGAAAAGGCG 
GAGCGGAAAT ACACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286954.1
CDS	250..1347
Translation	MSRNRAAMVSAFRLFLRPATTTTHRSLALRLAPGTTFALHLRPCHELQQHRSFASTAEDGETDKHKKPTTGDIYVEYVNGMPHMTVRLPSRNELCQFALKPISHNVGDLLAMLRAEDRGIDRAAVINKHGVRIASSCTIESLLDDSFSIQINNRTLDVNPPKRDKVTLESMDKVGDVRKVIAQLYEAFNVGEYQLEKSNQLAKELETLRYELEPLEEKKLELSKKAARRTNFMTWMGLGLMSVQFGILARLTWWEYSWDIMEPVTYFVTYGTTMAMYAYYCVTKREYMMEDVKNREFSLSLYRNAKKVQFDVEHYNELKRKSAELEYNLRRINDPLNMQLPSHLVRTQENTPPTLTEEKAERKYT

Target ORF information:

RefSeq Version	NM_001300025.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial calcium uniporter, transcript variant I (MCU), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300025.1

ORF Insert Sequence:

ATGTCCAGAA ATAGGGCTGC CATGGTCAGC GCCTTTCGGC TCTTCCTGCG ACCAGCGACC 
ACCACCACCC ATAGAAGTCT CGCCCTGCGT TTGGCCCCCG GCACCACCTT CGCCCTGCAC 
TTACGGCCAT GTCATGAGCT TCAGCAGCAC AGAAGCTTCG CATCCACGGC GGAGGATGGC 
GAAACGGACA AGCATAAAAA ACCGACGACG GGAGACATCT ATGTGGAATA CGTGAATGGC 
ATGCCACACA TGACTGTACG ACTTCCCAGT CGCAATGAAC TCTGCCAGTT TGCCCTGAAA 
CCCATCTCCC ACAACGTTGG TGATCTGTTA GCCATGCTCA GGGCTGAGGA TCGGGGCATC 
GATCGAGCTG CGGTGATCAA CAAGCATGGT GTGCGAATCG CGTCGTCCTG CACCATCGAA 
AGCTTGCTGG ATGACTCGTT TAGCATACAA ATCAACAATC GCACATTGGA TGTGAATCCT 
CCCAAACGTG ACAAAGTCAC ATTGGAGTCC ATGGACAAAG TGGGCGATGT TCGTAAGGTT 
ATTGCTCAGC TCTACGAGGC ATTCAACGTC GGAGAATATC AGCTGGAGAA GAGCAATCAA 
TTGGCCAAAG AACTGGAGAC CCTACGCTAT GAACTAGAAC CACTGGAAGA GAAAAAACTG 
GAACTCAGCA AAAAGGCCGC TCGTCGCACT AACTTTATGA CTTGGATGGG TTTGGGCCTA 
ATGTCGGTGC AATTTGGCAT TTTGGCACGA CTGACTTGGT GGGAATACTC TTGGGATATT 
ATGGAACCCG TTACATATTT TGTTACTTAC GGGACGACAA TGGCAATGTA TGCCTACTAT 
TGTGTCACTA AGAGGGAATA TATGATGGAG GATGTGAAGA ATCGCGAATT CTCACTTTCC 
CTTTATAGGA ACGCCAAAAA GGTTCAGTTC GATGTAGAAC ACTACAACGA ACTGAAGCGA 
AAGTCGGCGG AGTTGGAGTA CAATCTCAGA AGGATCAACG ACCCGCTCAA CATGCAATTG 
CCATCGCATT TGGTTCGGAC CCAGGAAAAC ACGCCACCAA CCTTAACTGA GGAAAAGGCG 
GAGCGGAAAT ACACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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MCU cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

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