clos cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	clos
Entrez Gene ID	5740204
Full Name	closca
Synonyms	CG12927,CG12928,CG1863,CG33479,CG34364,CG42600,Dmel\CG42600,Dmel_CG12927,Dmel_CG12928,Dmel_CG1863,Dmel_CG33479,Dmel_CG34364
General protein information	Preferred Names closca Names CG42600 gene product from transcript CG42600-RD CG42600-PD clos-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 45F6-45F6

mRNA and Protein(s)

mRNA	Protein	Name
NM_001103776.3	NP_001097246.3	closca

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011897	XP_320629.4
Drosophila melanogaster (fruit fly)	clos	NP_001097246.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following clos gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the clos cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12640	NM_001103776.3 Latest version!	Drosophila melanogaster closca (clos), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

clos ( NM_001103776.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097246.3 Drosophila melanogaster closca (clos), mRNA.

Price & Availability↑

CloneID	OFa12640
Clone ID Related Accession (Same CDS sequence)	NM_001103776.3
Accession Version	NM_001103776.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	closca
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On May 8, 2012 this sequence version replaced NM_001103776.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATCAAA TCTGTTGCAA TTTGTTGATC TTTGCGGCTG TGATCGCCCG CGTGATCAGT 
TTTGAAGTAA ACTTCCGGGA ATCCTATCCC ACACACTTCC TGAACTCAGA TCAGGAATCG 
ATGCTCCACA AACGGGCGAC TGCTGTGCCT CAGTTTTTCG ACCAAGCTAG CTTTAGTTTC 
GATGGACTAG AACGAGTGGC CTCCGTGCCG GTGTCTTTTC CCCTTGACAT TTGCATCCTC 
CAACTGCCCA CGGTCAAGTA CGCTACGGCC TTGCATAAAA ATGCCGCAGG AGTGCGTCAC 
TTTGCCGTCT ATACCTGGCG TCCAGCTGAG CAGGACTACC AGCTCCTCGT GGAGTTGGAA 
ACACCTAAGG CAGTGGCTCT GGATTGCCTG GCCTTCGCTG GGCGTGGCTA CGTAGCAGTG 
AGCTACAATC TAACAGAGCC CGTAAGCCAG GCCCGTGAAG GATCACCCAT CTATGAGATT 
TCCCCGGAAA CGGGGATACG GACAGTGCAA TACTTTTCGG GAACACATCT GCGTGGAATG 
TACCTTCGGA TCAGCAGCCA GGAACTTACT CTACTGCATG CCTTCGAATC GAATGCGCAG 
TGTCCCTACT TCAAGTGGAT GGGCAAGAGT TTCCAGAGAC TGGGCGCGAT TCACTGCAGC 
AAAGCTCGAA GAATGGAGGC CTTCGGCATT GACTACACCG ACTATGTGGC CGTAGCCAAC 
TACGCAGATG CGGAGGGACG AACTGCCACC CACTCAGAGA TCTTTCGATG GGATGCCAAG 
TCCCAGAGAT TCCAACTCTT CCAGCGACTG CGCAGCAATG GAGCCGTGGA CGTAAAGTAC 
TTCAGTCTCC CAGTAAACGA GGTCAGCCGC AGGCACTTTC TCATCCTGGG CAACACAATT 
GGCGGAACTG GCGCCGAGGT GGGCGATGCG GACACCGTGA TCTATGTCTT CGAAAAGGGG 
CAGTTTGTGC CGTATCAGCG ATTGAGTTTC TATGCCTTGG AAAGGGTTTT GCCAGTTCAG 
CACTCAATAT CGGAGAAGTT TCTACTCCTG GTTGCTTGTA ATAAACAGGA TGTCAAGATC 
TATAACCTTA ACGACTGGCG ATTCGAGGAA TCCAAGGTGC AGTTCACCGA GGGAGCTTTA 
AGTCGTGGTG TGGCACGTAT GCGAAGCTAT GAGGAGGGAG ATCAAAGCTA TTTGGTGATT 
GCGAATGAGA ATATGGCTGC CAACGAGACA AACATCTTTC AGCCACTCTA CAAACAAGAT 
GAGCATGCCA ACATACTTCG CCAGCAAATC ATTGACTGGG CTCGGGAGCA AAGAAAGCGA 
TTGGAGCAAA TGAATTTAGA TCATCTATTA ATAAGATTGA AAGACAAGTT AAAGCACCGT 
GAGGAGAAAC TGAAAGTGTC GATTATCAAG CAAGTGAACG CCAAATCGTT CGAGGATAAG 
CACATGAAAC TCACACCTAA TTACTGGAAG GCTTTGAAGC TAACCAAGCA GGCCTTGGAT 
AAAATCGAAC AAAAAACAAC CCTCGCAGGT TCCAGACATC CCAATAAGAG ATCCGTTGAT 
CAACCAAATG AAGAACCCAA TTTCGATGAG GTCACGGTGG ACACGCTGGT GGTGCACGGC 
GCAGTTAAAG CAGACCATAT CAATGGAGTT GATACGAGGA ACCCTGTTTA TGATTCCGTC 
ACTGCCAATA AGGTTTATGT TAGCGCAAAA TACACCGAAC CCGCCGGAAA AGAAAGAAAA 
CTTCTGCTGG ATGAGCTAAT AGTAAAGGAT CTGCAGCTTG ATGGGCGACT AAATGGGCTC 
AAGTGGGCAG ACCTGTTTGA TCTGACTTTA AAACGCAGCA ACAAAGATGT GCAGTTTATA 
AAGGCAGCTG CTGACTTCGA TCATCTGGAA TCCGACGCTG CCCAAGTCAA CAACAACGAG 
GTTAACGATA GACCGTTGGG TCTGCTAATC CCAGTGGATG GTGGAGACTT TATTGTCCAA 
CAAGATGTTC AGTTTTCCCA ACCAATTCAG GTGAATCGGC TGCTCATCAA TCAGCGGCTA 
AATCATATCC ACGTCGATCG TCAGCGCTTC GATGTCCTGA CCAAGCAGGC AAATCACACA 
CAGGTCATCG AGGGTTCCAT GCGATTCGAG AATGTTCGAG TCATGGAACC GATTACCATT 
GCGGGTCAAA TTATGGGGGC CGAACTGAGA GCGATGTCAC CGGCAAAGGT TACCCACAAG 
TCGCTTCAAC TTAATGGGGA TTTCGTAGTT GACGGCGATG TGACCATTGG ACGGCTGCTG 
CAAATGAAAG ATTTTGTGGA CGAATCTACT CAGAAATCTG CAGCAGAAGC ATTGACTCGG 
GGCCTGCGAT TGGATCAACC TTTGGAGGAT GTGAATCTCA ACTTTGTGGA ACCACTTATT 
GCCAACAATA CCGAGCTAAG CTTTATCAAC ACGCAAGATC TACAAAACCT GGTTAAGCTG 
AACGTGGGTG AAGTGCAGTT GGTGCAGGCA AGTAAAATAT TTCCTCAAAA TGTAGAGATA 
CGCGATGGAT TCGGTGAGAT CAAGTGGCTA AATGGGATTG ATACGGAAAG ACTCCCGGAA 
ATTATGCTCA CCAAGAGTGG CAACCAAACT ATTTCGAGTT CAATTCAATT ACAAGGCGTA 
GAACTGAACC ATGTCAATTC ATCACAGATC TTATTGAATG ATTTGGGAGT TGAGGATTAT 
CTTCAAACAG GTCAGGATCA AAAGTCAAAT GGAACACTGT ATATAGACAA CATGGATGTG 
AGGGAAATGA GCGTGAATGA TCTGCACCTA AATGGCCATC TCTTTGGCCA AACAATGTCC 
TCCATCTACG AACATGGAAG CAAGTCCCTG GACAGCTGGC ATCTGCCACC CGACTTCAAT 
GGCACCATTC ACGCCCAGAA TCTGTGGCTT AGTGGCCATA TTAACAAAGT GAAGGTGGTC 
CAATTGGAGC AGCAGCTGCA GCAACTGGCG GGCAATGTCA AATACGTTGG CGACTTTACG 
TTTGAGCATG ATGTAAACAT CAGTTCCCTC AGCTTTGAAA ACTCTCTAAA TGGAATTGAG 
GCCAATAAAT TTGGTAGCTG TTGGTTGGAG ACCGACAGGG ACCAGACTTT CACAGCCCCG 
CAGAAGCTGG CTTCCCTGGA CAGCCAGCAA GGTCTCTGGC TAAGAGGGCA ACTGAACAAC 
CACACCTTGC AAGATCTGGA GAACCGTAGC TACAGGCTCA ACTCACCGGA GCACCTGCAA 
TCAGTGCGAT TTGCGAATCC CATTGTGTTG CAATCGGAGC TACAGTTGGA TCGCTTAAAT 
GGACTCCGCG TCCCAGAGGA CGTTATTTAC CATAATGGTG GAGCTTACAT CTCTGCTCCC 
GTTAGCGTAG ATGGTGACCT GGAGGCTTTA AAATTGTCCA ACATAACCAA CCTGAATGGT 
TATCCTCTGG AGGCGTTGGA GATCTATTTA AGTGGGCAAA ATGTGCGCAC CTTTGTGGCG 
GAGAATGTTA AGTTTGGCGG AGAACCCTCT TATCAGCTCC TGAATGGCCA CAATCTAGGA 
AACCTTCTTG ATCAGGTGTG GCTGGACAAC GAGCAGATTG AGTTGAGAGA AATAAAACTG 
GAATCTGGGC AATTCGAAGG CCTGCTGGAA TTCCAGGGAC CCATCAACGG GCACCAAGTG 
GATCATATAA CTCACAACTA CTTCAGCCGG ACGCGTAGCA ATCTGCTGAA AACTCCCCTG 
AGCTTCCAGC ATGACGTCAC GTTCGCACAG CCTCCAGTTG CCAAATACGT AAAACTACGA 
CGTGGCAGCA GCGGCGTTTT AATGGAGGGC ACCAGTAGCA ACTTCTCCCT AGATTTTGAT 
GATTTTGTAA CTCACTCGTT GAAAACAGCC GGCTCTCACG CTATAAGTGG ATATTGGAAA 
CTGCCTGAGG CCTTTATTTC GGGAAATCTA AACGATATTT ACATAAATAA TTTAAACCTT 
GCTAATGATG TCGTTAGAAT GCCTCTTCAA AATGACAGTA TCAATCTTCC CTTGATAGTC 
GAAGCATTGA AAACTGTCCA GGGCGGCACT ATAAATCGTC TCCATACAAC CCCTGAAAGC 
CAGGTGGCGG ATGTGCCTGT GGCCAAGTGG ATCCATGGAG CAGCCTACCT TTACGGGAAC 
CACAGTATTT CTGGCATTAC TCATCTGAAT AATGTCAATC TCTACAACGA TCTTACCGTG 
AACGGGTCTG TAAATGGTGT CCCCTGGCAG GCGGACAAGC TCATTTTGAT GGACAATGAG 
CAGGATATAC CGGGCTCCCT GCTGGTGGCG AATAGTCTTC CCGAACAGAA GCGAGTTATG 
AGCAATAATG TGAAGAATCT CTGGGTGGAT TTCGTCAACG ATCTGCCCGT TAACGAGTTA 
CTGGTCAGCA AGGCAGAAAA CCGTCCCAAT CTTCACGTGG AAAGCCAACT GATCTTCACC 
CAGCCCCTCA CTGTGGGTCA CTACGAAGCG GGCAACGGAA ATGGGCTGCT GGATAAATAT 
TACAAGAAAA AGCGGGGTGT CGAATCGAAT GACATTCATG ATTGGCAGCA GCTGCAGGAA 
AATGTGGCAG CTATTAAAAA CAGATTGGCC AAGCCGCCTA AAGTGCTGGA AGGCTTTGCT 
GTGCTGCAGA AATTACCTCA TAAGGCCTCG CACATGAAGA TCATCTCCGT GGACAACGAG 
GACGTACTGG CCATCTGGGA GCAGATGCCC AGCGAAGTAG GGACTACTTA CACATGGCAA 
CCGGATATAC ATCTTTTTGG GCGAAGTTCA AGCACAGCAC TGACACAACT AAACAAGACA 
AACTTGAATC CAATCAATGA GGAGGCACAG AACCCGCAGA AACTGCAATT CAGATCATAT 
GGATTTCAGT GCCGGGCACT TCAAGAGGAG AACCAGATAG TAATCCGATG CCTAGATGAG 
CAGAACAACA GCAGGGAAGC AATCTTGCCC AGGCGGGCCG TAAAACAGTT GTTGCCGGTC 
GCTGATTCAG ATGATATGAC CATTCTACTT CGTACTCCCA GAGCGGTGGA GCTTTGGAAG 
TGGAGTAATG GCAATTACTC TCTATCAAGC AGGCTGATAG ATGGGCAGGT GGAGCAAATG 
GCTCTTAGTC AAGGAGGTGC TGGCATAGAG AGCGGATATG TGGCGCTGAT GGCTTCATCG 
CCAGCAGCAG AGATTCGGAT TTACAGCTTC GGCAGCCCAG ACACGAGCAG CTTCCAACTG 
GACCAAGTCC TGGAGCTCGA GGACTCCCAA CAGTCGCGGG TAATGCGCTT TATGCACCTG 
CCCGAGTCCG ACGATCTACT GCTCTGTGTG TCCAACGTAC TGCCGCAGCA ACCGCTTCGC 
ATCTACCAGC ACCGCGGAGC AGCGGGATTC CAGCAAATCC TCGGCGACAG CGCCTTGCCA 
AAGGCTCATG CCCTGGAGGT GCTCTATCTG CCCAGCCATA AGCTGCGACT GCTGTCCATG 
GCCACGGAGG AGTCGGTCTA CTTGCTGCAG CCACAGTTTA CTACACTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097246.3
CDS	58..5568
Translation	MYQICCNLLIFAAVIARVISFEVNFRESYPTHFLNSDQESMLHKRATAVPQFFDQASFSFDGLERVASVPVSFPLDICILQLPTVKYATALHKNAAGVRHFAVYTWRPAEQDYQLLVELETPKAVALDCLAFAGRGYVAVSYNLTEPVSQAREGSPIYEISPETGIRTVQYFSGTHLRGMYLRISSQELTLLHAFESNAQCPYFKWMGKSFQRLGAIHCSKARRMEAFGIDYTDYVAVANYADAEGRTATHSEIFRWDAKSQRFQLFQRLRSNGAVDVKYFSLPVNEVSRRHFLILGNTIGGTGAEVGDADTVIYVFEKGQFVPYQRLSFYALERVLPVQHSISEKFLLLVACNKQDVKIYNLNDWRFEESKVQFTEGALSRGVARMRSYEEGDQSYLVIANENMAANETNIFQPLYKQDEHANILRQQIIDWAREQRKRLEQMNLDHLLIRLKDKLKHREEKLKVSIIKQVNAKSFEDKHMKLTPNYWKALKLTKQALDKIEQKTTLAGSRHPNKRSVDQPNEEPNFDEVTVDTLVVHGAVKADHINGVDTRNPVYDSVTANKVYVSAKYTEPAGKERKLLLDELIVKDLQLDGRLNGLKWADLFDLTLKRSNKDVQFIKAAADFDHLESDAAQVNNNEVNDRPLGLLIPVDGGDFIVQQDVQFSQPIQVNRLLINQRLNHIHVDRQRFDVLTKQANHTQVIEGSMRFENVRVMEPITIAGQIMGAELRAMSPAKVTHKSLQLNGDFVVDGDVTIGRLLQMKDFVDESTQKSAAEALTRGLRLDQPLEDVNLNFVEPLIANNTELSFINTQDLQNLVKLNVGEVQLVQASKIFPQNVEIRDGFGEIKWLNGIDTERLPEIMLTKSGNQTISSSIQLQGVELNHVNSSQILLNDLGVEDYLQTGQDQKSNGTLYIDNMDVREMSVNDLHLNGHLFGQTMSSIYEHGSKSLDSWHLPPDFNGTIHAQNLWLSGHINKVKVVQLEQQLQQLAGNVKYVGDFTFEHDVNISSLSFENSLNGIEANKFGSCWLETDRDQTFTAPQKLASLDSQQGLWLRGQLNNHTLQDLENRSYRLNSPEHLQSVRFANPIVLQSELQLDRLNGLRVPEDVIYHNGGAYISAPVSVDGDLEALKLSNITNLNGYPLEALEIYLSGQNVRTFVAENVKFGGEPSYQLLNGHNLGNLLDQVWLDNEQIELREIKLESGQFEGLLEFQGPINGHQVDHITHNYFSRTRSNLLKTPLSFQHDVTFAQPPVAKYVKLRRGSSGVLMEGTSSNFSLDFDDFVTHSLKTAGSHAISGYWKLPEAFISGNLNDIYINNLNLANDVVRMPLQNDSINLPLIVEALKTVQGGTINRLHTTPESQVADVPVAKWIHGAAYLYGNHSISGITHLNNVNLYNDLTVNGSVNGVPWQADKLILMDNEQDIPGSLLVANSLPEQKRVMSNNVKNLWVDFVNDLPVNELLVSKAENRPNLHVESQLIFTQPLTVGHYEAGNGNGLLDKYYKKKRGVESNDIHDWQQLQENVAAIKNRLAKPPKVLEGFAVLQKLPHKASHMKIISVDNEDVLAIWEQMPSEVGTTYTWQPDIHLFGRSSSTALTQLNKTNLNPINEEAQNPQKLQFRSYGFQCRALQEENQIVIRCLDEQNNSREAILPRRAVKQLLPVADSDDMTILLRTPRAVELWKWSNGNYSLSSRLIDGQVEQMALSQGGAGIESGYVALMASSPAAEIRIYSFGSPDTSSFQLDQVLELEDSQQSRVMRFMHLPESDDLLLCVSNVLPQQPLRIYQHRGAAGFQQILGDSALPKAHALEVLYLPSHKLRLLSMATEESVYLLQPQFTTL

Target ORF information:

RefSeq Version	NM_001103776.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster closca (clos), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103776.3

ORF Insert Sequence:

ATGTATCAAA TCTGTTGCAA TTTGTTGATC TTTGCGGCTG TGATCGCCCG CGTGATCAGT 
TTTGAAGTAA ACTTCCGGGA ATCCTATCCC ACACACTTCC TGAACTCAGA TCAGGAATCG 
ATGCTCCACA AACGGGCGAC TGCTGTGCCT CAGTTTTTCG ACCAAGCTAG CTTTAGTTTC 
GATGGACTAG AACGAGTGGC CTCCGTGCCG GTGTCTTTTC CCCTTGACAT TTGCATCCTC 
CAACTGCCCA CGGTCAAGTA CGCTACGGCC TTGCATAAAA ATGCCGCAGG AGTGCGTCAC 
TTTGCCGTCT ATACCTGGCG TCCAGCTGAG CAGGACTACC AGCTCCTCGT GGAGTTGGAA 
ACACCTAAGG CAGTGGCTCT GGATTGCCTG GCCTTCGCTG GGCGTGGCTA CGTAGCAGTG 
AGCTACAATC TAACAGAGCC CGTAAGCCAG GCCCGTGAAG GATCACCCAT CTATGAGATT 
TCCCCGGAAA CGGGGATACG GACAGTGCAA TACTTTTCGG GAACACATCT GCGTGGAATG 
TACCTTCGGA TCAGCAGCCA GGAACTTACT CTACTGCATG CCTTCGAATC GAATGCGCAG 
TGTCCCTACT TCAAGTGGAT GGGCAAGAGT TTCCAGAGAC TGGGCGCGAT TCACTGCAGC 
AAAGCTCGAA GAATGGAGGC CTTCGGCATT GACTACACCG ACTATGTGGC CGTAGCCAAC 
TACGCAGATG CGGAGGGACG AACTGCCACC CACTCAGAGA TCTTTCGATG GGATGCCAAG 
TCCCAGAGAT TCCAACTCTT CCAGCGACTG CGCAGCAATG GAGCCGTGGA CGTAAAGTAC 
TTCAGTCTCC CAGTAAACGA GGTCAGCCGC AGGCACTTTC TCATCCTGGG CAACACAATT 
GGCGGAACTG GCGCCGAGGT GGGCGATGCG GACACCGTGA TCTATGTCTT CGAAAAGGGG 
CAGTTTGTGC CGTATCAGCG ATTGAGTTTC TATGCCTTGG AAAGGGTTTT GCCAGTTCAG 
CACTCAATAT CGGAGAAGTT TCTACTCCTG GTTGCTTGTA ATAAACAGGA TGTCAAGATC 
TATAACCTTA ACGACTGGCG ATTCGAGGAA TCCAAGGTGC AGTTCACCGA GGGAGCTTTA 
AGTCGTGGTG TGGCACGTAT GCGAAGCTAT GAGGAGGGAG ATCAAAGCTA TTTGGTGATT 
GCGAATGAGA ATATGGCTGC CAACGAGACA AACATCTTTC AGCCACTCTA CAAACAAGAT 
GAGCATGCCA ACATACTTCG CCAGCAAATC ATTGACTGGG CTCGGGAGCA AAGAAAGCGA 
TTGGAGCAAA TGAATTTAGA TCATCTATTA ATAAGATTGA AAGACAAGTT AAAGCACCGT 
GAGGAGAAAC TGAAAGTGTC GATTATCAAG CAAGTGAACG CCAAATCGTT CGAGGATAAG 
CACATGAAAC TCACACCTAA TTACTGGAAG GCTTTGAAGC TAACCAAGCA GGCCTTGGAT 
AAAATCGAAC AAAAAACAAC CCTCGCAGGT TCCAGACATC CCAATAAGAG ATCCGTTGAT 
CAACCAAATG AAGAACCCAA TTTCGATGAG GTCACGGTGG ACACGCTGGT GGTGCACGGC 
GCAGTTAAAG CAGACCATAT CAATGGAGTT GATACGAGGA ACCCTGTTTA TGATTCCGTC 
ACTGCCAATA AGGTTTATGT TAGCGCAAAA TACACCGAAC CCGCCGGAAA AGAAAGAAAA 
CTTCTGCTGG ATGAGCTAAT AGTAAAGGAT CTGCAGCTTG ATGGGCGACT AAATGGGCTC 
AAGTGGGCAG ACCTGTTTGA TCTGACTTTA AAACGCAGCA ACAAAGATGT GCAGTTTATA 
AAGGCAGCTG CTGACTTCGA TCATCTGGAA TCCGACGCTG CCCAAGTCAA CAACAACGAG 
GTTAACGATA GACCGTTGGG TCTGCTAATC CCAGTGGATG GTGGAGACTT TATTGTCCAA 
CAAGATGTTC AGTTTTCCCA ACCAATTCAG GTGAATCGGC TGCTCATCAA TCAGCGGCTA 
AATCATATCC ACGTCGATCG TCAGCGCTTC GATGTCCTGA CCAAGCAGGC AAATCACACA 
CAGGTCATCG AGGGTTCCAT GCGATTCGAG AATGTTCGAG TCATGGAACC GATTACCATT 
GCGGGTCAAA TTATGGGGGC CGAACTGAGA GCGATGTCAC CGGCAAAGGT TACCCACAAG 
TCGCTTCAAC TTAATGGGGA TTTCGTAGTT GACGGCGATG TGACCATTGG ACGGCTGCTG 
CAAATGAAAG ATTTTGTGGA CGAATCTACT CAGAAATCTG CAGCAGAAGC ATTGACTCGG 
GGCCTGCGAT TGGATCAACC TTTGGAGGAT GTGAATCTCA ACTTTGTGGA ACCACTTATT 
GCCAACAATA CCGAGCTAAG CTTTATCAAC ACGCAAGATC TACAAAACCT GGTTAAGCTG 
AACGTGGGTG AAGTGCAGTT GGTGCAGGCA AGTAAAATAT TTCCTCAAAA TGTAGAGATA 
CGCGATGGAT TCGGTGAGAT CAAGTGGCTA AATGGGATTG ATACGGAAAG ACTCCCGGAA 
ATTATGCTCA CCAAGAGTGG CAACCAAACT ATTTCGAGTT CAATTCAATT ACAAGGCGTA 
GAACTGAACC ATGTCAATTC ATCACAGATC TTATTGAATG ATTTGGGAGT TGAGGATTAT 
CTTCAAACAG GTCAGGATCA AAAGTCAAAT GGAACACTGT ATATAGACAA CATGGATGTG 
AGGGAAATGA GCGTGAATGA TCTGCACCTA AATGGCCATC TCTTTGGCCA AACAATGTCC 
TCCATCTACG AACATGGAAG CAAGTCCCTG GACAGCTGGC ATCTGCCACC CGACTTCAAT 
GGCACCATTC ACGCCCAGAA TCTGTGGCTT AGTGGCCATA TTAACAAAGT GAAGGTGGTC 
CAATTGGAGC AGCAGCTGCA GCAACTGGCG GGCAATGTCA AATACGTTGG CGACTTTACG 
TTTGAGCATG ATGTAAACAT CAGTTCCCTC AGCTTTGAAA ACTCTCTAAA TGGAATTGAG 
GCCAATAAAT TTGGTAGCTG TTGGTTGGAG ACCGACAGGG ACCAGACTTT CACAGCCCCG 
CAGAAGCTGG CTTCCCTGGA CAGCCAGCAA GGTCTCTGGC TAAGAGGGCA ACTGAACAAC 
CACACCTTGC AAGATCTGGA GAACCGTAGC TACAGGCTCA ACTCACCGGA GCACCTGCAA 
TCAGTGCGAT TTGCGAATCC CATTGTGTTG CAATCGGAGC TACAGTTGGA TCGCTTAAAT 
GGACTCCGCG TCCCAGAGGA CGTTATTTAC CATAATGGTG GAGCTTACAT CTCTGCTCCC 
GTTAGCGTAG ATGGTGACCT GGAGGCTTTA AAATTGTCCA ACATAACCAA CCTGAATGGT 
TATCCTCTGG AGGCGTTGGA GATCTATTTA AGTGGGCAAA ATGTGCGCAC CTTTGTGGCG 
GAGAATGTTA AGTTTGGCGG AGAACCCTCT TATCAGCTCC TGAATGGCCA CAATCTAGGA 
AACCTTCTTG ATCAGGTGTG GCTGGACAAC GAGCAGATTG AGTTGAGAGA AATAAAACTG 
GAATCTGGGC AATTCGAAGG CCTGCTGGAA TTCCAGGGAC CCATCAACGG GCACCAAGTG 
GATCATATAA CTCACAACTA CTTCAGCCGG ACGCGTAGCA ATCTGCTGAA AACTCCCCTG 
AGCTTCCAGC ATGACGTCAC GTTCGCACAG CCTCCAGTTG CCAAATACGT AAAACTACGA 
CGTGGCAGCA GCGGCGTTTT AATGGAGGGC ACCAGTAGCA ACTTCTCCCT AGATTTTGAT 
GATTTTGTAA CTCACTCGTT GAAAACAGCC GGCTCTCACG CTATAAGTGG ATATTGGAAA 
CTGCCTGAGG CCTTTATTTC GGGAAATCTA AACGATATTT ACATAAATAA TTTAAACCTT 
GCTAATGATG TCGTTAGAAT GCCTCTTCAA AATGACAGTA TCAATCTTCC CTTGATAGTC 
GAAGCATTGA AAACTGTCCA GGGCGGCACT ATAAATCGTC TCCATACAAC CCCTGAAAGC 
CAGGTGGCGG ATGTGCCTGT GGCCAAGTGG ATCCATGGAG CAGCCTACCT TTACGGGAAC 
CACAGTATTT CTGGCATTAC TCATCTGAAT AATGTCAATC TCTACAACGA TCTTACCGTG 
AACGGGTCTG TAAATGGTGT CCCCTGGCAG GCGGACAAGC TCATTTTGAT GGACAATGAG 
CAGGATATAC CGGGCTCCCT GCTGGTGGCG AATAGTCTTC CCGAACAGAA GCGAGTTATG 
AGCAATAATG TGAAGAATCT CTGGGTGGAT TTCGTCAACG ATCTGCCCGT TAACGAGTTA 
CTGGTCAGCA AGGCAGAAAA CCGTCCCAAT CTTCACGTGG AAAGCCAACT GATCTTCACC 
CAGCCCCTCA CTGTGGGTCA CTACGAAGCG GGCAACGGAA ATGGGCTGCT GGATAAATAT 
TACAAGAAAA AGCGGGGTGT CGAATCGAAT GACATTCATG ATTGGCAGCA GCTGCAGGAA 
AATGTGGCAG CTATTAAAAA CAGATTGGCC AAGCCGCCTA AAGTGCTGGA AGGCTTTGCT 
GTGCTGCAGA AATTACCTCA TAAGGCCTCG CACATGAAGA TCATCTCCGT GGACAACGAG 
GACGTACTGG CCATCTGGGA GCAGATGCCC AGCGAAGTAG GGACTACTTA CACATGGCAA 
CCGGATATAC ATCTTTTTGG GCGAAGTTCA AGCACAGCAC TGACACAACT AAACAAGACA 
AACTTGAATC CAATCAATGA GGAGGCACAG AACCCGCAGA AACTGCAATT CAGATCATAT 
GGATTTCAGT GCCGGGCACT TCAAGAGGAG AACCAGATAG TAATCCGATG CCTAGATGAG 
CAGAACAACA GCAGGGAAGC AATCTTGCCC AGGCGGGCCG TAAAACAGTT GTTGCCGGTC 
GCTGATTCAG ATGATATGAC CATTCTACTT CGTACTCCCA GAGCGGTGGA GCTTTGGAAG 
TGGAGTAATG GCAATTACTC TCTATCAAGC AGGCTGATAG ATGGGCAGGT GGAGCAAATG 
GCTCTTAGTC AAGGAGGTGC TGGCATAGAG AGCGGATATG TGGCGCTGAT GGCTTCATCG 
CCAGCAGCAG AGATTCGGAT TTACAGCTTC GGCAGCCCAG ACACGAGCAG CTTCCAACTG 
GACCAAGTCC TGGAGCTCGA GGACTCCCAA CAGTCGCGGG TAATGCGCTT TATGCACCTG 
CCCGAGTCCG ACGATCTACT GCTCTGTGTG TCCAACGTAC TGCCGCAGCA ACCGCTTCGC 
ATCTACCAGC ACCGCGGAGC AGCGGGATTC CAGCAAATCC TCGGCGACAG CGCCTTGCCA 
AAGGCTCATG CCCTGGAGGT GCTCTATCTG CCCAGCCATA AGCTGCGACT GCTGTCCATG 
GCCACGGAGG AGTCGGTCTA CTTGCTGCAG CCACAGTTTA CTACACTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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clos cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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