Hsp60B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hsp60B
Entrez Gene ID	48572
Full Name	Heat shock protein 60B
Synonyms	152031_at,CG2830,Dm21D,Dmel\CG2830,Hsp60b,Hsp64,anon-WO0140519.61,hsp60B,ms(2)06619,ms(2)21D
General protein information	Preferred Names Heat shock protein 60B Names CG2830 gene product from transcript CG2830-RA CG2830-PA Hsp 60 related Hsp60B-PA heat shock protein 60 related heat shock protein 60B male sterile(2)21D
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 21E2-21E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_080186.4	NP_524925.1	heat shock protein 60B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hsp60B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hsp60B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15268	NM_080186.4 Latest version!	Drosophila melanogaster heat shock protein 60B (Hsp60B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hsp60B ( NM_080186.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524925.1 Drosophila melanogaster heat shock protein 60B (Hsp60B), mRNA.

Price & Availability↑

CloneID	OFa15268
Clone ID Related Accession (Same CDS sequence)	NM_080186.4
Accession Version	NM_080186.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heat shock protein 60B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_080186.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTCGGT CGTGTGTGCC CAAGGCGATT ACCTCTAGTC GCTGTTTCGC GCGGATGTAC 
TCGAAGGACG TGCGCTTTGG CTCCGGAGTA CGGGCAATGA TGATCCGGGG CGTTGATATT 
CTGGCTGATG CCGTTGCTGT GACCATGGGG CCCAAGGGTC GCAGTGTGAT CGTGGAGCGA 
CCATGGACCT CGCCGAAGAT CACCAAGGAC GGCTTCACGG TCGCGCGCTC GATTGCCCTA 
AAGGACCAGC ACATGAATCT GGGCGCCAAG TTGGTCCAGG ATGTGGCCGA TAATACGAAT 
GAGTCGGCGG GTGACGGCAC GACCACAGCA ACAGTTTTGG CTCGAGCGAT TGCCAAGGAA 
GGATTCAATC AGATTACCAT GGGGGCTAAT CCCGTGGAGA TTCGTCGAGG TGTCATGTTA 
GCTGTTGACG TCGTAAAGGA CAAGCTGAAG GAAATGTCCA AGGCAGTGGA AACGCGTGAG 
GAGATCCAAC AAGTAGCCAC CCTCTCCGCG AACGGGGATA CTGAAATTGG GCGGCTCATT 
GGTGAGGCCA CCGACAAAGT GGGTCCAAGA GGAACTATTA CTGTAAAGGA TGGCAAGCGC 
CTGAAGGACG AGCTAAATAT TATCCAGGGC CTGCGCTTCG ACAACGGCTA TGTGTCGCCC 
TTTTTCGTAA ACAGCTCCAA GGGGTCCAAG GTGGAGTTTG CCAACGCCCT TGTAATGATA 
TCCCTGAAGA AGATCACTGG ACTCTCGCAA ATAGTTAAAG GTCTGGAGCA ATCCCTGAGG 
CAGCGTCGTC CCCTGATCAT CATAGCCGAG GACATCAGCG GGGAAGCCCT CAATGCTTTG 
GTCCTGAACA AGCTCAGGTT GGGCCTCCAG GTGTGCGCCG TCAAATCGCC CAGCTATGGA 
CACCACCGCA AGGAGCTTAT AGGCGATATA TCCGCTGCCA CGGGAGCAAC TATTTTCGGC 
GATGACATCA ACTATTCCAA AATGGAGGAG GCTAAGCTGG AGGATCTGGG TCAGGTTGGC 
GAGGCTGTGA TTAGCAAGGA CAGCACCATG CTCCTGCAGG GCAAACCCAA GACAGGACTT 
TTGGAAATGC GCATCCAGCA AATTCAAGAT GAACTGGCGG AAAAGCAAAT CAAGCCAGAG 
CAGCGGGATC GTCTGCGCCA ACGCCTCTCC GCCCTCACGA AGGGCGTGGC AGTGCTGCAC 
ATTGGTGGAG GCAGTGAAGT TGAGGTGAAT GAGAAAAAGG ACCGTGTGGT GGACGCCTTG 
AATGCCACTC GAGCGGCCAT CGAAGAGGGA ATTGTCCCAG GTGGCGGTAC GGCCTTTTTG 
CGCTGCATTC CCTACCTCCA GGAACTCAAA ACGGAGAGTG CAGATCTCCA GAAGGGCGTC 
GATATCGTCT GCAATGCCCT GAGGATGCCT TGCCAGACCA TAGCTCAAAA CGCCGGAGTT 
GATGGCCCAA TGGTGGTGGC CAAGGTGTTG AATGGCTCGG AAGACTACGG CTACGATGCC 
ATGGGCGATG AGTACTGTCG GCTGGTCGAG AAGGGCATTA TTGATCCCAC AAAGGTGCTG 
CGGACCGCCA TCACGGATGC AGCTGGAGTG GCCTCTCTTC TGAGCACCAC CGAGGTGGTT 
ATCACCGACT CCCGGAACGA TGATCTTTTG TCCAAGTTGT CAGGAGCTGG CGGCGGAATG 
GATGATGGAC TGGACATGAA CATGGGTGGC TTAGAGGAGC TGGCAGCCTT AAGTGGATTG 
GGTGGCATGG GTGGTATGGG AGGCATGGGT GGTATGGGAG GTATGGGAGG AATGGGAGGT 
GGATTCGGCG GAATGGGCGC TGGTGGCGGA ATGAGCGCTT CTGCCAGCAA TGACGGTCCC 
ACTGCCGAGG AGATGAATGA AATGGTCAAG GCTATTCCCG GCATGGAGCA AGTAGAGGTG 
CGTGACATTG ACAGTGGGAT GATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524925.1
CDS	147..2093
Translation	MFRSCVPKAITSSRCFARMYSKDVRFGSGVRAMMIRGVDILADAVAVTMGPKGRSVIVERPWTSPKITKDGFTVARSIALKDQHMNLGAKLVQDVADNTNESAGDGTTTATVLARAIAKEGFNQITMGANPVEIRRGVMLAVDVVKDKLKEMSKAVETREEIQQVATLSANGDTEIGRLIGEATDKVGPRGTITVKDGKRLKDELNIIQGLRFDNGYVSPFFVNSSKGSKVEFANALVMISLKKITGLSQIVKGLEQSLRQRRPLIIIAEDISGEALNALVLNKLRLGLQVCAVKSPSYGHHRKELIGDISAATGATIFGDDINYSKMEEAKLEDLGQVGEAVISKDSTMLLQGKPKTGLLEMRIQQIQDELAEKQIKPEQRDRLRQRLSALTKGVAVLHIGGGSEVEVNEKKDRVVDALNATRAAIEEGIVPGGGTAFLRCIPYLQELKTESADLQKGVDIVCNALRMPCQTIAQNAGVDGPMVVAKVLNGSEDYGYDAMGDEYCRLVEKGIIDPTKVLRTAITDAAGVASLLSTTEVVITDSRNDDLLSKLSGAGGGMDDGLDMNMGGLEELAALSGLGGMGGMGGMGGMGGMGGMGGGFGGMGAGGGMSASASNDGPTAEEMNEMVKAIPGMEQVEVRDIDSGMM

Target ORF information:

RefSeq Version	NM_080186.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heat shock protein 60B (Hsp60B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080186.4

ORF Insert Sequence:

ATGTTTCGGT CGTGTGTGCC CAAGGCGATT ACCTCTAGTC GCTGTTTCGC GCGGATGTAC 
TCGAAGGACG TGCGCTTTGG CTCCGGAGTA CGGGCAATGA TGATCCGGGG CGTTGATATT 
CTGGCTGATG CCGTTGCTGT GACCATGGGG CCCAAGGGTC GCAGTGTGAT CGTGGAGCGA 
CCATGGACCT CGCCGAAGAT CACCAAGGAC GGCTTCACGG TCGCGCGCTC GATTGCCCTA 
AAGGACCAGC ACATGAATCT GGGCGCCAAG TTGGTCCAGG ATGTGGCCGA TAATACGAAT 
GAGTCGGCGG GTGACGGCAC GACCACAGCA ACAGTTTTGG CTCGAGCGAT TGCCAAGGAA 
GGATTCAATC AGATTACCAT GGGGGCTAAT CCCGTGGAGA TTCGTCGAGG TGTCATGTTA 
GCTGTTGACG TCGTAAAGGA CAAGCTGAAG GAAATGTCCA AGGCAGTGGA AACGCGTGAG 
GAGATCCAAC AAGTAGCCAC CCTCTCCGCG AACGGGGATA CTGAAATTGG GCGGCTCATT 
GGTGAGGCCA CCGACAAAGT GGGTCCAAGA GGAACTATTA CTGTAAAGGA TGGCAAGCGC 
CTGAAGGACG AGCTAAATAT TATCCAGGGC CTGCGCTTCG ACAACGGCTA TGTGTCGCCC 
TTTTTCGTAA ACAGCTCCAA GGGGTCCAAG GTGGAGTTTG CCAACGCCCT TGTAATGATA 
TCCCTGAAGA AGATCACTGG ACTCTCGCAA ATAGTTAAAG GTCTGGAGCA ATCCCTGAGG 
CAGCGTCGTC CCCTGATCAT CATAGCCGAG GACATCAGCG GGGAAGCCCT CAATGCTTTG 
GTCCTGAACA AGCTCAGGTT GGGCCTCCAG GTGTGCGCCG TCAAATCGCC CAGCTATGGA 
CACCACCGCA AGGAGCTTAT AGGCGATATA TCCGCTGCCA CGGGAGCAAC TATTTTCGGC 
GATGACATCA ACTATTCCAA AATGGAGGAG GCTAAGCTGG AGGATCTGGG TCAGGTTGGC 
GAGGCTGTGA TTAGCAAGGA CAGCACCATG CTCCTGCAGG GCAAACCCAA GACAGGACTT 
TTGGAAATGC GCATCCAGCA AATTCAAGAT GAACTGGCGG AAAAGCAAAT CAAGCCAGAG 
CAGCGGGATC GTCTGCGCCA ACGCCTCTCC GCCCTCACGA AGGGCGTGGC AGTGCTGCAC 
ATTGGTGGAG GCAGTGAAGT TGAGGTGAAT GAGAAAAAGG ACCGTGTGGT GGACGCCTTG 
AATGCCACTC GAGCGGCCAT CGAAGAGGGA ATTGTCCCAG GTGGCGGTAC GGCCTTTTTG 
CGCTGCATTC CCTACCTCCA GGAACTCAAA ACGGAGAGTG CAGATCTCCA GAAGGGCGTC 
GATATCGTCT GCAATGCCCT GAGGATGCCT TGCCAGACCA TAGCTCAAAA CGCCGGAGTT 
GATGGCCCAA TGGTGGTGGC CAAGGTGTTG AATGGCTCGG AAGACTACGG CTACGATGCC 
ATGGGCGATG AGTACTGTCG GCTGGTCGAG AAGGGCATTA TTGATCCCAC AAAGGTGCTG 
CGGACCGCCA TCACGGATGC AGCTGGAGTG GCCTCTCTTC TGAGCACCAC CGAGGTGGTT 
ATCACCGACT CCCGGAACGA TGATCTTTTG TCCAAGTTGT CAGGAGCTGG CGGCGGAATG 
GATGATGGAC TGGACATGAA CATGGGTGGC TTAGAGGAGC TGGCAGCCTT AAGTGGATTG 
GGTGGCATGG GTGGTATGGG AGGCATGGGT GGTATGGGAG GTATGGGAGG AATGGGAGGT 
GGATTCGGCG GAATGGGCGC TGGTGGCGGA ATGAGCGCTT CTGCCAGCAA TGACGGTCCC 
ACTGCCGAGG AGATGAATGA AATGGTCAAG GCTATTCCCG GCATGGAGCA AGTAGAGGTG 
CGTGACATTG ACAGTGGGAT GATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Hsp60B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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