Zw10 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Zw10
Entrez Gene ID	47874
Full Name	Zeste-white 10
Synonyms	CG9900,Dmel\CG9900,Dsl1,EG:BACR7C10.3,Mit(1)15,ZW-10,ZW10,abe,dZW10,l(1)3Af,l(1)6-99,l(1)zw10,mit(1)15,mit(l)15,zw-10,zw10
General protein information	Preferred Names Zeste-white 10 Names CG9900 gene product from transcript CG9900-RB CG9900-PB Zw10-PB abnormal-eye mitotic 15
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 3A6-3A7\|1-1.2 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_080162.4	NP_524901.2	Zeste-white 10

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	mit(1)15	NP_524901.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004467	XP_313765.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ZW10 has roles in mitotic checkpoint control, dynein targeting and membrane trafficking with dynein [review]
Title: ZW10 function in mitotic checkpoint control, dynein targeting and membrane trafficking: is dynein the unifying theme?

Rod/Zw10 contributes to checkpoint activation by promoting Mad2 recruitment and to checkpoint inactivation by recruiting dynein/dynactin that subsequently removes Mad2 from attached kinetochores.
Title: Recruitment of Mad2 to the kinetochore requires the Rod/Zw10 complex.

Zwilch localizes to kinetochores and kinetochore microtubules in a manner identical to ZW10 and ROD.
Title: Zwilch, a new component of the ZW10/ROD complex required for kinetochore functions.

The following Zw10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Zw10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa02416	NM_080162.4 Latest version!	Drosophila melanogaster Zeste-white 10 (Zw10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Zw10 ( NM_080162.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524901.2 Drosophila melanogaster Zeste-white 10 (Zw10), mRNA.

Price & Availability↑

CloneID	OFa02416
Clone ID Related Accession (Same CDS sequence)	NM_080162.4
Accession Version	NM_080162.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Zeste-white 10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_080162.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAAG AGGCGCCGCG GTTCAATGTG CTGGAGGAGG CGTTCAACGG CAATGGGAAC 
GGGTGCGCCA ACGTGGAGGC CACCCAATCG GCCATATTAA AAGTGCTGAC GCGCGTTAAT 
CGCTTCCAGA TGCGCGTCCG CAAGCACATC GAAGACAACT ACACGGAGTT TTTGCCCAAC 
AACACGTCGC CGGACATATT TCTGGAGGAG TCGGGGTCCT TGAATCGCGA GATTCACGAC 
ATGCTCGAGA ACTTGGGTTC CGAGGGACTG GACGCCTTGG ACGAGGCGAA TGTCAAGATG 
GCGGGTAACG GCAGGCAGCT GCGAGAGATC CTTTTGGGTC TGGGTGTCAG CGAGCATGTG 
CTAAGGATCG ACGAGCTGTT CCAGTGCGTG GAGGAGGCCA AGGCCACCAA GGATTACTTG 
GTGTTACTGG ACCTTGTCGG TCGTTTGAGG GCTTTCATCT ATGGCGATGA TTCCGTTGAC 
GGGGACGCGC AGGTGGCCAC GCCAGAGGTT CGACGCATCT TTAAGGCTCT CGAGTGCTAC 
GAGACCATCA AGGTGAAATA CCATGTACAG GCATATATGC TGCAGCAGAG CCTCCAGGAG 
CGCTTCGATC GCCTGGTGCA GCTGCAGTGC AAATCCTTTC CCACATCGCG TTGCGTCACC 
TTGCAGGTGA GCCGGGATCA AACGCAGCTG CAGGATATTG TGCAGGCTCT CTTCCAGGAG 
CCCTACAATC CGGCGCGCCT CTGCGAATTC CTGCTGGACA ATTGCATAGA ACCGGTGATC 
ATGCGCCCAG TGATGGCCGA TTACAGCGAA GAAGCCGATG GTGGCACCTA CGTTCGATTG 
TCGCTTTCCT ACGCCACCAA GGAGCCCAGC TCAGCGCAGC TTCGCCCGAA CTACAAGCAG 
GTCTTGGAGA ACCTCAGGCT GCTGCTGCAC ACGCTGGCCG GGATCAACTG CAGTGTGTCC 
AGGGACCAAC ATGTTTTTGG CATTATTGGC GATCATGTGA AGGATAAAAT GCTGAAATTA 
CTGGTGGACG AGTGCCTGAT ACCAGCTGTG CCCGAAAGCA CGGAGGAGTA TCAGACATCC 
ACGCTGTGTG AGGATGTCGC CCAGCTGGAG CAGCTGCTTG TAGACTCATT CATCATCAAT 
CCCGAGCAAG ATCGAGCCCT AGGGCAGTTT GTAGAGAAGT ACGAGACCTA CTACCGCAAT 
CGGATGTATC GTCGAGTTCT GGAAACGGCG CGGGAGATCA TCCAGCGCGA TCTGCAGGAC 
ATGGTGCTGG TGGCGCCCAA CAACCACTCA GCCGAAGTGG CAAACGATCC CTTCCTCTTC 
CCACGCTGCA TGATCTCGAA AAGTGCTCAG GACTTTGTCA AACTAATGGA CCGCATTCTT 
CGCCAGCCCA CGGATAAACT GGGCGACCAA GAGGCCGATC CCATAGCCGG CGTCATTTCC 
ATCATGCTGC ACACCTACAT CAATGAGGTG CCCAAGGTGC ACCGCAAGCT GCTCGAGAGC 
ATTCCACAGC AGGCCGTCCT GTTCCACAAC AATTGTATGT TCTTCACACA CTGGGTAGCG 
CAGCATGCGA ACAAGGGCAT CGAAAGCTTG GCGGCGCTGG CCAAGACACT GCAGGCCACC 
GGTCAGCAGC ATTTCCGCGT GCAGGTCGAC TACCAATCCT CCATCCTGAT GGGCATCATG 
CAGGAGTTCG AGTTCGAGAG CACGCACACG CTGGGCTCTG GTCCACTGAA GCTGGTGCGT 
CAGTGCCTTC GCCAGCTGGA GCTGCTGAAG AACGTGTGGG CCAATGTGCT CCCGGAGACC 
GTGTACAATG CAACCTTCTG CGAGCTAATC AACACATTTG TCGCCGAGCT AATCCGTCGA 
GTGTTCACGC TGCGCGACAT TTCCGCACAG ATGGCCTGTG AGCTGAGCGA TCTCATTGAC 
GTGGTGCTCC AGCGGGCGCC CACGCTTTTC CGCGAACCAA ACGAGGTGGT CCAGGTGCTC 
TCTTGGCTTA AGCTGCAGCA ACTGAAGGCC ATGCTGAACG CGTCGCTCAT GGAGATCACA 
GAGCTGTGGG GCGACGGCGT CGGCCCGCTG ACCGCCAGCT ACAAGTCGGA TGAGATAAAG 
CACCTAATCA GGGCGTTGTT CCAGGATACG GATTGGCGGG CCAAGGCCAT TACGCAGATT 
GTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524901.2
CDS	107..2272
Translation	MEEEAPRFNVLEEAFNGNGNGCANVEATQSAILKVLTRVNRFQMRVRKHIEDNYTEFLPNNTSPDIFLEESGSLNREIHDMLENLGSEGLDALDEANVKMAGNGRQLREILLGLGVSEHVLRIDELFQCVEEAKATKDYLVLLDLVGRLRAFIYGDDSVDGDAQVATPEVRRIFKALECYETIKVKYHVQAYMLQQSLQERFDRLVQLQCKSFPTSRCVTLQVSRDQTQLQDIVQALFQEPYNPARLCEFLLDNCIEPVIMRPVMADYSEEADGGTYVRLSLSYATKEPSSAQLRPNYKQVLENLRLLLHTLAGINCSVSRDQHVFGIIGDHVKDKMLKLLVDECLIPAVPESTEEYQTSTLCEDVAQLEQLLVDSFIINPEQDRALGQFVEKYETYYRNRMYRRVLETAREIIQRDLQDMVLVAPNNHSAEVANDPFLFPRCMISKSAQDFVKLMDRILRQPTDKLGDQEADPIAGVISIMLHTYINEVPKVHRKLLESIPQQAVLFHNNCMFFTHWVAQHANKGIESLAALAKTLQATGQQHFRVQVDYQSSILMGIMQEFEFESTHTLGSGPLKLVRQCLRQLELLKNVWANVLPETVYNATFCELINTFVAELIRRVFTLRDISAQMACELSDLIDVVLQRAPTLFREPNEVVQVLSWLKLQQLKAMLNASLMEITELWGDGVGPLTASYKSDEIKHLIRALFQDTDWRAKAITQIV

Target ORF information:

RefSeq Version	NM_080162.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Zeste-white 10 (Zw10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080162.4

ORF Insert Sequence:

ATGGAGGAAG AGGCGCCGCG GTTCAATGTG CTGGAGGAGG CGTTCAACGG CAATGGGAAC 
GGGTGCGCCA ACGTGGAGGC CACCCAATCG GCCATATTAA AAGTGCTGAC GCGCGTTAAT 
CGCTTCCAGA TGCGCGTCCG CAAGCACATC GAAGACAACT ACACGGAGTT TTTGCCCAAC 
AACACGTCGC CGGACATATT TCTGGAGGAG TCGGGGTCCT TGAATCGCGA GATTCACGAC 
ATGCTCGAGA ACTTGGGTTC CGAGGGACTG GACGCCTTGG ACGAGGCGAA TGTCAAGATG 
GCGGGTAACG GCAGGCAGCT GCGAGAGATC CTTTTGGGTC TGGGTGTCAG CGAGCATGTG 
CTAAGGATCG ACGAGCTGTT CCAGTGCGTG GAGGAGGCCA AGGCCACCAA GGATTACTTG 
GTGTTACTGG ACCTTGTCGG TCGTTTGAGG GCTTTCATCT ATGGCGATGA TTCCGTTGAC 
GGGGACGCGC AGGTGGCCAC GCCAGAGGTT CGACGCATCT TTAAGGCTCT CGAGTGCTAC 
GAGACCATCA AGGTGAAATA CCATGTACAG GCATATATGC TGCAGCAGAG CCTCCAGGAG 
CGCTTCGATC GCCTGGTGCA GCTGCAGTGC AAATCCTTTC CCACATCGCG TTGCGTCACC 
TTGCAGGTGA GCCGGGATCA AACGCAGCTG CAGGATATTG TGCAGGCTCT CTTCCAGGAG 
CCCTACAATC CGGCGCGCCT CTGCGAATTC CTGCTGGACA ATTGCATAGA ACCGGTGATC 
ATGCGCCCAG TGATGGCCGA TTACAGCGAA GAAGCCGATG GTGGCACCTA CGTTCGATTG 
TCGCTTTCCT ACGCCACCAA GGAGCCCAGC TCAGCGCAGC TTCGCCCGAA CTACAAGCAG 
GTCTTGGAGA ACCTCAGGCT GCTGCTGCAC ACGCTGGCCG GGATCAACTG CAGTGTGTCC 
AGGGACCAAC ATGTTTTTGG CATTATTGGC GATCATGTGA AGGATAAAAT GCTGAAATTA 
CTGGTGGACG AGTGCCTGAT ACCAGCTGTG CCCGAAAGCA CGGAGGAGTA TCAGACATCC 
ACGCTGTGTG AGGATGTCGC CCAGCTGGAG CAGCTGCTTG TAGACTCATT CATCATCAAT 
CCCGAGCAAG ATCGAGCCCT AGGGCAGTTT GTAGAGAAGT ACGAGACCTA CTACCGCAAT 
CGGATGTATC GTCGAGTTCT GGAAACGGCG CGGGAGATCA TCCAGCGCGA TCTGCAGGAC 
ATGGTGCTGG TGGCGCCCAA CAACCACTCA GCCGAAGTGG CAAACGATCC CTTCCTCTTC 
CCACGCTGCA TGATCTCGAA AAGTGCTCAG GACTTTGTCA AACTAATGGA CCGCATTCTT 
CGCCAGCCCA CGGATAAACT GGGCGACCAA GAGGCCGATC CCATAGCCGG CGTCATTTCC 
ATCATGCTGC ACACCTACAT CAATGAGGTG CCCAAGGTGC ACCGCAAGCT GCTCGAGAGC 
ATTCCACAGC AGGCCGTCCT GTTCCACAAC AATTGTATGT TCTTCACACA CTGGGTAGCG 
CAGCATGCGA ACAAGGGCAT CGAAAGCTTG GCGGCGCTGG CCAAGACACT GCAGGCCACC 
GGTCAGCAGC ATTTCCGCGT GCAGGTCGAC TACCAATCCT CCATCCTGAT GGGCATCATG 
CAGGAGTTCG AGTTCGAGAG CACGCACACG CTGGGCTCTG GTCCACTGAA GCTGGTGCGT 
CAGTGCCTTC GCCAGCTGGA GCTGCTGAAG AACGTGTGGG CCAATGTGCT CCCGGAGACC 
GTGTACAATG CAACCTTCTG CGAGCTAATC AACACATTTG TCGCCGAGCT AATCCGTCGA 
GTGTTCACGC TGCGCGACAT TTCCGCACAG ATGGCCTGTG AGCTGAGCGA TCTCATTGAC 
GTGGTGCTCC AGCGGGCGCC CACGCTTTTC CGCGAACCAA ACGAGGTGGT CCAGGTGCTC 
TCTTGGCTTA AGCTGCAGCA ACTGAAGGCC ATGCTGAACG CGTCGCTCAT GGAGATCACA 
GAGCTGTGGG GCGACGGCGT CGGCCCGCTG ACCGCCAGCT ACAAGTCGGA TGAGATAAAG 
CACCTAATCA GGGCGTTGTT CCAGGATACG GATTGGCGGG CCAAGGCCAT TACGCAGATT 
GTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Zw10 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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