fs(1)M3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	fs(1)M3
Entrez Gene ID	45994
Full Name	female sterile (1) M3
Synonyms	CG4790,Dmel\CG4790,Fs(1)K646,fs(1)K646,fs(1)ph,fs(1)phl,fs(1)pole hole,fs(1)polehole
General protein information	Preferred Names female sterile (1) M3 Names CG4790 gene product from transcript CG4790-RA CG4790-PA fs(1)M3-PA fs(1)polehole polehole
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 5D1-5D1\|1-15 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_132056.5	NP_572284.4	female sterile (1) M3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nasrat and Polehole are essential for extracellular accumulation of Torso-like, a factor secreted during oogenesis that initiates Torso receptor activation (Polehole protein)
Title: Cell surface proteins Nasrat and Polehole stabilize the Torso-like extracellular determinant in Drosophila oogenesis.

Required for cross-linkage of the vitelline membrane.
Title: Drosophila vitelline membrane cross-linking requires the fs(1)Nasrat, fs(1)polehole and chorion genes activities.

The following fs(1)M3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fs(1)M3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa02399	NM_132056.5 Latest version!	Drosophila melanogaster female sterile (1) M3 (fs(1)M3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fs(1)M3 ( NM_132056.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572284.4 Drosophila melanogaster female sterile (1) M3 (fs(1)M3), mRNA.

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CloneID	OFa02399
Clone ID Related Accession (Same CDS sequence)	NM_132056.5
Accession Version	NM_132056.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	female sterile (1) M3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132056.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTTCGC CAAGCGTGTG TGCAGCATTA TTTAGTGTGT TAGTGTTAAA TAGCTATATC 
GCCCAAGTAC AAAGTGAATT ATCAGCAGCA GAAGTCGCTG GAGATCCGAG TGCTGCCCAG 
CTGCTGCCCA GTTCCTGTCG CAATGGGTTG AATGGCAGTA GCTCTGGCCC GGAAATCCGT 
CTGCCCTTTG TGGACATGAT GGTGCGCCAT GAGAAGCCAC TCTTTGTGCT CCGCGAACCG 
GAAGAGGATC TACTTTTACT GCTCCATGGA CACGAGATTT TTCAGCTGCA CGTGGCCACT 
GGGGAGAGGC GTCTAGTGCC GCTTGGTTAC TTGGCGGGCC AGGTCAGTGG TCGCAGAGCT 
CTCGGAGCAG AGGTTGTGGC CTGGCGGGAG TTCCTGCTCC TTGCCATCGT TCTGGATGAT 
TCCATGGAGG TTTACCAGTT GCCCAAGGAA CTGCTCCTAT CCGAAGATCC AGCCACACAG 
GTGAGCTTTG AGCCCCTCCA GGAGTTCTCG CTGCCAGGTG CTTTTCTACA GCTGCACCTT 
TTGAAACCCA GTGGAGATCA GGTGCTCCTC CTAGTGGCCT CCAATCACAC CAGATCACAC 
AGCAAATGCA GAACCTTTGA GTGGCTGGAC ACCTACTTTA ATCCACTCGA GGAGATCACT 
CTACCCGCCA TTCGCGTTCT CCAAGTGGTT GGTCGTCAGC CACTTTATAT CATTTTTGGT 
CGTTCTTTAA AGGGTTCCCA GTCAAAGCTG GTGCTCACAA TCTACGAGCT GGATAGAAAC 
AGCCGAATCC TGCAACAACG CCAGGCTTTG ACAGTTCAGG CGCAAAGTGT CCAAGCTGTT 
AGATTTCACG GGAGGAACTG CCTCATCGCC TGTACTTCCT CTGCATCTGC CTCGTGTATT 
TTCTTCCGAA TGGTGGATGG ACAATTTGTG GTGTACCGAA AGCATGCTCG AAAAGATTTG 
AGTTTCCGTC GATTGAGTGC CACCAAAAAG GGTCACCTTT TGATTGGTGC CCGTGCCAAT 
GGCGAGGTGA TCGTCTTCAG CTCCACTCGC CTGGATTGCT ATAGTGGATT TCAGGTGGCT 
GGAGAAGCGG ATCCAAGTGG ATTACTTATT CATAAAAACT TGCACAATGA GACCTTTCTT 
TTGCTGGCCT ATCGAAGATC CTCGAGTGAT CTGATCCGAA CTGTGCAATT GGGTGGCGTG 
GAAGAAATCT CCAACCAAGT GGGCGCTGCT CCAGATGAAG ATCTAAGTGT TGTCCAATTG 
CATCGTCACG AGTTTGAGGA GTCCATCAAT GCCTTACGTG GCCTCCTGCT GCGCCGTCGT 
TCCTCTACAG AAAACCTGAG AAACATTGTG GACATCTTAA AACGCAAATC GGCCATCCAC 
CTCGACACGC CACTGCATCT AGTGCAGCAA GGACAAATAG GAAGACTTCA GTTGGTGGAC 
GAACATCTGC GCACGCCCAC CCAATTAAAG CAACGCTTGG AGGAACTTCG TCAACGATTT 
AAAGCGATCC AGACCAGGCG AACCACACGC TCTTTTGGGA CAGGAAACGA GAACGATGAG 
CCGGAGACAC TCAAAGCGCG ACGATTGCGC GTGGGCAATC TTATCTACAA GGGACAGCTA 
ATTTCAGGCC ACATTTTGGA TAACTCAAAA CCACCTATGT TGACCATCAG AAACAGTCCT 
TTGCAGACGA AAAAGCTTAG GGTAAATCAA TTGGTTACTC CCCAAGATTT CCGCAACTCC 
AGTGGTGCCC AGATTGCGCC TGAGGAAACC AGTGGCAGTG AATGTGTAGT TCGTCATCTC 
CGAGTGGAAC AGATAAACGG AGTGCAGTGG AACGACTTTT TGGACTCGCT CTTCCTGCGC 
AGTCGTGACA CTTCGATAGA AGGACGCTTG GTTATGCAAT CGCGAGTCCG GGTGAACAGT 
CTAAAGACAC CTCTGCTCAA CGGACTCGTG GTGGATCGTC TCTTTAATCT GCGGCGTGCC 
CAGGTGATCA GTTCGAATAT CTACATGTCC GCCTTCTTTG CTCCGCGTTT GGAGGCTCAG 
CAAGTAAATG GACTTGACTT TGCCCACGAT GTACTTCATC GCGGTAGCAA TGATACATGG 
ATAAAAACTC CTGTTCGCAT CAATCAGATG AGTGTTTCGG GTGACATTTT TGTGGGCAAT 
ACGAGCAAGT GGCAGCCACA GCCCAGACAA CTCGACGATC CAATGGAACA ACGTCTTCAA 
CAGTACTACA CGGGCAGGGT AACCATCCGC GGATCCCTGA CGGTCACAAA TGTCCTGCGA 
GACACGGCAA ACTCACTTAT AGTGCTCGGA AACCAATCTC TCGCCAGAAA AGATATACAA 
TCTACGTACC TTCTTGATCA GACGCCACAG AACCTCACTA ACTTGACCTT TGGACATGCC 
AGAGTTAGTG TTCCCTCAAT GACCACCAGT TATGTTGACG GTCATCCTGT GAGGGATCAT 
CTCCTAAGCG GAGGACAGCA GAACCAACAT ACTCACCCAA ATAACACTCT ACACATTATC 
TTTATGAATG CAACCATACT GGGCGATGTT ATTTGCAGGG ATTACAGTTC CCGTTTGGCG 
GAGATCGCAA AAGATGCAGT GAGTCATGGA GACGCTGTTA ATATCACGGG GCACAAACAC 
TTCCAGGCAC CTTTGACTGT TCAAGCACTT CAAGCCAATG AAATAAACGG AGTACCTGTT 
AGTGAAATAG TGGTGAAATC AAACACCACC CAAAACTTTA CGGGTACAAA GACCTTTGCC 
CGTGTGGTGG TGGCGAATGA TATCAACGTT CATGATCATT TGAATGTGAG TCATCTTAAT 
GGATTGCCAT TGCAACAGCT GCTTGGTCAC GATATCAGCC TGCAACGCCT CGAACTAACC 
GAAACCCCAC ATCTGGAGAA TCTTCATTTT CGGCGACTTA ATGGATTGCC CTTCGATGAA 
CTGCTGTCCA AAATTTCGGA AGATGGTGAA CAGCCCCTAC TTCTTCACAA GCAACTATTG 
ATCGAAGGAA ATGTACGATT TGACAAGGCT GTCCAAGTGC AGAACATTAA TGGAATCCAA 
TGGGACGAGT ACCTAGATCG CCTGGTGAGA TCTGATTCTA ACGCGGAAAT ACGGGGTAAA 
AAGACATTCA TGGCCGATGT CCAAATAAAT GATGCCCTGC AAAGTCCTCA CATCAATAAT 
TTGGATCTAA GTTCCCTGCT CGACAACACC TTACTGCGAA TGACACCCCA GCAAATCAGT 
GGTGCTTACA CTTTCGACCG CATGATGGCC ACCAATGTGG ATGTAAATCG AATAAATGGG 
GTACCCAAGG AGGAATTTAT AGACACACGC AAAGACTTAG AACTAAAAGG AGATCTTTAC 
CTAAAGCAAC TGAACATCAA TGGCAGCCTA AAGTGTCCCT TTGTCAATGA GGCAGTTTTA 
AATGATCTCA AGCAACGAGT GGAGCAAGTG CAGCAACTTC CCTGGCGGAA TTTGATAGTC 
ACCGGCGATG CTCTATGGGA TGGTGAAGAG CAGTCCCAAT CCCAGCTAGA CTACCTACGC 
CAGCATGCAG TCAGGAGAGT GGGCAACCAG ACCATTGCCG GACACGTTCT ACTGAAGGAA 
CCCTACTTAA AGGATATCCA AAGTAAGCAT TCCCTGCCAA TTGATCTTAA CTTCACTCAA 
TTGGCCGAAG ATGCCCTACT TCGCCGGAGC CCCACAAACG AAACCCAGGT GATAACAGCT 
CCGCAAGAGC TACAGGGATT AGTTAGAGCT AATACTCTAC ACTTGGAGCA GGATGCACAC 
TTTGAGCTGG TAAACGGAAT CGATATACGA CGATTCAATG CCTCACTGTA CCGCCTCTCC 
AGTGGAGAAG CCATTGCAGC GGATCTTCAC TTTCATCAGG CTCCAAAAAT AGGCAAACTT 
CAACTGGAGT ATCCAGAAGT GAATGGAGCT CCGATACAAA GTATATACCA GCAGGGCAAG 
GGGCAATCCT GGCCACCCGT AACGTTCAAG AACCTCTTTG TCGAACAGGA CCTGAATCTG 
AAAACCGTAA ACAACATGAA TCTGGACTAC TTTCTACAAC ATCGCATTCC CTTAAAGGGA 
GACGCATTGG AGGTATTCGG TTCACTGACT TTTGAGCACC TTCAGCTGAG TGGAAAGCCT 
CTTCTACGTT CTATCAATGG CATTCAATTG GATAACTTGG TGTTTCGGCA TAGCAATCGG 
ACGCAGAGCA TCACGGGTGC CAAGACTTTT CGTGGAGGAG TGGAGTTCAC TGGACCCGGT 
CATGTGATGA ATCTGAATGG ACGTGAGCTT AGCGAAAGTT TCAGGGGCAG CATCTTTCGA 
AATAAGGACT ACAACATCGA TAGTCTAGTT CTGGACAAAG CTCTCTTCCC AGGTGGACTA 
GTTTTAGCAG GACTTCAAAG TCAAATTCCC AATCGAATGC GAGCGACGGA AGTGCAAAGC 
AATCCGTTAG AGGAGTTTCA AGAACTTCTA GCATTGGGTA ATCAAACTTC GCCTAGGAGG 
CTACTTTACC TGGATCACGA CACCGAAAGT CTGGAGTCTA CTTGGGTAAA AGGCCCTTTT 
AGGGGATCTT CGGAACTGCA GGTGAGCCTG CCCTTGGGTT ATTCTGCTCC ATGTCAGCGT 
AGGACTTTAC AAGCCAAGCT CCTGATCCAC GAACGTCGCG TCCTCCTGGC CAACTCGAGT 
GTGTCCAATC AACTGATGAG ACTTCACTCC GGAGATATCC GGGTGAAGGT GCAGAACCAC 
TGTCATCGCC CAGCACGAAG ATTGCGCAGC AGGATCAGAA TTAGTTGCCG TAATGAAACG 
CATACGCTGG GAATGCGTCA ATCAGTGGAA GCCATGGAAC TGCTGGAAAT GGGGGAAATG 
GTGCTGCTGC TCTTGGGCAC CGAGGAGGAA GTTCGTGTCC TGCGTCTGAA GAGGAGCAAC 
TGTAGTCTCA CCGATTGGCA ATCCGTGCTA CCAGCAGATG GGCGCCTTAT GAAGTTGGTT 
CGAATGGGGG TAGAGTCCCG ACAGGATCTA ATACTCACCA GTGGCATGAG TAATCATCGT 
CCTGTCCTGG CCATCCATTA TAGGGATCCC TTAAATCATA ACTTCAAAAT GCTTCAGCTG 
ATCCAGGGCT CATATGACCT GGCAGAGATG CAGGGAAACC AACTTCTGGT CAGCTGTTTG 
GGTTGTCGAC ACATAGCTAT CTTTAAACGG TACTCTCATT CCGCGGAAAT TCTGTTTGAG 
CCCATCCAGC AGTTGAGCTT CCAGGAGCGC ATTCAACAAC TAACTCCATT CAAAGTTGGA 
AATGAGCAAT ATCTATTGGT AGTAACGCAG CCGGAGGAAG AACACTTTTA CCTCTTCAGC 
TATAATGAAG TAGGCGGTTG GCAACAGCGT ACGTTTGGCA TTAAGAAACA GGACCAGTGG 
GCGTGGTCAC TGGTGAAATC TGGTCAAATG CTTGAAGCCG CCGAGACCCC TATTCTGCTG 
CTCTGCGGAA AGAAACGGGA ATGTAGTCTA GTGAAAGCCC TACTGGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572284.4
CDS	91..5601
Translation	MISPSVCAALFSVLVLNSYIAQVQSELSAAEVAGDPSAAQLLPSSCRNGLNGSSSGPEIRLPFVDMMVRHEKPLFVLREPEEDLLLLLHGHEIFQLHVATGERRLVPLGYLAGQVSGRRALGAEVVAWREFLLLAIVLDDSMEVYQLPKELLLSEDPATQVSFEPLQEFSLPGAFLQLHLLKPSGDQVLLLVASNHTRSHSKCRTFEWLDTYFNPLEEITLPAIRVLQVVGRQPLYIIFGRSLKGSQSKLVLTIYELDRNSRILQQRQALTVQAQSVQAVRFHGRNCLIACTSSASASCIFFRMVDGQFVVYRKHARKDLSFRRLSATKKGHLLIGARANGEVIVFSSTRLDCYSGFQVAGEADPSGLLIHKNLHNETFLLLAYRRSSSDLIRTVQLGGVEEISNQVGAAPDEDLSVVQLHRHEFEESINALRGLLLRRRSSTENLRNIVDILKRKSAIHLDTPLHLVQQGQIGRLQLVDEHLRTPTQLKQRLEELRQRFKAIQTRRTTRSFGTGNENDEPETLKARRLRVGNLIYKGQLISGHILDNSKPPMLTIRNSPLQTKKLRVNQLVTPQDFRNSSGAQIAPEETSGSECVVRHLRVEQINGVQWNDFLDSLFLRSRDTSIEGRLVMQSRVRVNSLKTPLLNGLVVDRLFNLRRAQVISSNIYMSAFFAPRLEAQQVNGLDFAHDVLHRGSNDTWIKTPVRINQMSVSGDIFVGNTSKWQPQPRQLDDPMEQRLQQYYTGRVTIRGSLTVTNVLRDTANSLIVLGNQSLARKDIQSTYLLDQTPQNLTNLTFGHARVSVPSMTTSYVDGHPVRDHLLSGGQQNQHTHPNNTLHIIFMNATILGDVICRDYSSRLAEIAKDAVSHGDAVNITGHKHFQAPLTVQALQANEINGVPVSEIVVKSNTTQNFTGTKTFARVVVANDINVHDHLNVSHLNGLPLQQLLGHDISLQRLELTETPHLENLHFRRLNGLPFDELLSKISEDGEQPLLLHKQLLIEGNVRFDKAVQVQNINGIQWDEYLDRLVRSDSNAEIRGKKTFMADVQINDALQSPHINNLDLSSLLDNTLLRMTPQQISGAYTFDRMMATNVDVNRINGVPKEEFIDTRKDLELKGDLYLKQLNINGSLKCPFVNEAVLNDLKQRVEQVQQLPWRNLIVTGDALWDGEEQSQSQLDYLRQHAVRRVGNQTIAGHVLLKEPYLKDIQSKHSLPIDLNFTQLAEDALLRRSPTNETQVITAPQELQGLVRANTLHLEQDAHFELVNGIDIRRFNASLYRLSSGEAIAADLHFHQAPKIGKLQLEYPEVNGAPIQSIYQQGKGQSWPPVTFKNLFVEQDLNLKTVNNMNLDYFLQHRIPLKGDALEVFGSLTFEHLQLSGKPLLRSINGIQLDNLVFRHSNRTQSITGAKTFRGGVEFTGPGHVMNLNGRELSESFRGSIFRNKDYNIDSLVLDKALFPGGLVLAGLQSQIPNRMRATEVQSNPLEEFQELLALGNQTSPRRLLYLDHDTESLESTWVKGPFRGSSELQVSLPLGYSAPCQRRTLQAKLLIHERRVLLANSSVSNQLMRLHSGDIRVKVQNHCHRPARRLRSRIRISCRNETHTLGMRQSVEAMELLEMGEMVLLLLGTEEEVRVLRLKRSNCSLTDWQSVLPADGRLMKLVRMGVESRQDLILTSGMSNHRPVLAIHYRDPLNHNFKMLQLIQGSYDLAEMQGNQLLVSCLGCRHIAIFKRYSHSAEILFEPIQQLSFQERIQQLTPFKVGNEQYLLVVTQPEEEHFYLFSYNEVGGWQQRTFGIKKQDQWAWSLVKSGQMLEAAETPILLLCGKKRECSLVKALLG

Target ORF information:

RefSeq Version	NM_132056.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster female sterile (1) M3 (fs(1)M3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132056.5

ORF Insert Sequence:

ATGATTTCGC CAAGCGTGTG TGCAGCATTA TTTAGTGTGT TAGTGTTAAA TAGCTATATC 
GCCCAAGTAC AAAGTGAATT ATCAGCAGCA GAAGTCGCTG GAGATCCGAG TGCTGCCCAG 
CTGCTGCCCA GTTCCTGTCG CAATGGGTTG AATGGCAGTA GCTCTGGCCC GGAAATCCGT 
CTGCCCTTTG TGGACATGAT GGTGCGCCAT GAGAAGCCAC TCTTTGTGCT CCGCGAACCG 
GAAGAGGATC TACTTTTACT GCTCCATGGA CACGAGATTT TTCAGCTGCA CGTGGCCACT 
GGGGAGAGGC GTCTAGTGCC GCTTGGTTAC TTGGCGGGCC AGGTCAGTGG TCGCAGAGCT 
CTCGGAGCAG AGGTTGTGGC CTGGCGGGAG TTCCTGCTCC TTGCCATCGT TCTGGATGAT 
TCCATGGAGG TTTACCAGTT GCCCAAGGAA CTGCTCCTAT CCGAAGATCC AGCCACACAG 
GTGAGCTTTG AGCCCCTCCA GGAGTTCTCG CTGCCAGGTG CTTTTCTACA GCTGCACCTT 
TTGAAACCCA GTGGAGATCA GGTGCTCCTC CTAGTGGCCT CCAATCACAC CAGATCACAC 
AGCAAATGCA GAACCTTTGA GTGGCTGGAC ACCTACTTTA ATCCACTCGA GGAGATCACT 
CTACCCGCCA TTCGCGTTCT CCAAGTGGTT GGTCGTCAGC CACTTTATAT CATTTTTGGT 
CGTTCTTTAA AGGGTTCCCA GTCAAAGCTG GTGCTCACAA TCTACGAGCT GGATAGAAAC 
AGCCGAATCC TGCAACAACG CCAGGCTTTG ACAGTTCAGG CGCAAAGTGT CCAAGCTGTT 
AGATTTCACG GGAGGAACTG CCTCATCGCC TGTACTTCCT CTGCATCTGC CTCGTGTATT 
TTCTTCCGAA TGGTGGATGG ACAATTTGTG GTGTACCGAA AGCATGCTCG AAAAGATTTG 
AGTTTCCGTC GATTGAGTGC CACCAAAAAG GGTCACCTTT TGATTGGTGC CCGTGCCAAT 
GGCGAGGTGA TCGTCTTCAG CTCCACTCGC CTGGATTGCT ATAGTGGATT TCAGGTGGCT 
GGAGAAGCGG ATCCAAGTGG ATTACTTATT CATAAAAACT TGCACAATGA GACCTTTCTT 
TTGCTGGCCT ATCGAAGATC CTCGAGTGAT CTGATCCGAA CTGTGCAATT GGGTGGCGTG 
GAAGAAATCT CCAACCAAGT GGGCGCTGCT CCAGATGAAG ATCTAAGTGT TGTCCAATTG 
CATCGTCACG AGTTTGAGGA GTCCATCAAT GCCTTACGTG GCCTCCTGCT GCGCCGTCGT 
TCCTCTACAG AAAACCTGAG AAACATTGTG GACATCTTAA AACGCAAATC GGCCATCCAC 
CTCGACACGC CACTGCATCT AGTGCAGCAA GGACAAATAG GAAGACTTCA GTTGGTGGAC 
GAACATCTGC GCACGCCCAC CCAATTAAAG CAACGCTTGG AGGAACTTCG TCAACGATTT 
AAAGCGATCC AGACCAGGCG AACCACACGC TCTTTTGGGA CAGGAAACGA GAACGATGAG 
CCGGAGACAC TCAAAGCGCG ACGATTGCGC GTGGGCAATC TTATCTACAA GGGACAGCTA 
ATTTCAGGCC ACATTTTGGA TAACTCAAAA CCACCTATGT TGACCATCAG AAACAGTCCT 
TTGCAGACGA AAAAGCTTAG GGTAAATCAA TTGGTTACTC CCCAAGATTT CCGCAACTCC 
AGTGGTGCCC AGATTGCGCC TGAGGAAACC AGTGGCAGTG AATGTGTAGT TCGTCATCTC 
CGAGTGGAAC AGATAAACGG AGTGCAGTGG AACGACTTTT TGGACTCGCT CTTCCTGCGC 
AGTCGTGACA CTTCGATAGA AGGACGCTTG GTTATGCAAT CGCGAGTCCG GGTGAACAGT 
CTAAAGACAC CTCTGCTCAA CGGACTCGTG GTGGATCGTC TCTTTAATCT GCGGCGTGCC 
CAGGTGATCA GTTCGAATAT CTACATGTCC GCCTTCTTTG CTCCGCGTTT GGAGGCTCAG 
CAAGTAAATG GACTTGACTT TGCCCACGAT GTACTTCATC GCGGTAGCAA TGATACATGG 
ATAAAAACTC CTGTTCGCAT CAATCAGATG AGTGTTTCGG GTGACATTTT TGTGGGCAAT 
ACGAGCAAGT GGCAGCCACA GCCCAGACAA CTCGACGATC CAATGGAACA ACGTCTTCAA 
CAGTACTACA CGGGCAGGGT AACCATCCGC GGATCCCTGA CGGTCACAAA TGTCCTGCGA 
GACACGGCAA ACTCACTTAT AGTGCTCGGA AACCAATCTC TCGCCAGAAA AGATATACAA 
TCTACGTACC TTCTTGATCA GACGCCACAG AACCTCACTA ACTTGACCTT TGGACATGCC 
AGAGTTAGTG TTCCCTCAAT GACCACCAGT TATGTTGACG GTCATCCTGT GAGGGATCAT 
CTCCTAAGCG GAGGACAGCA GAACCAACAT ACTCACCCAA ATAACACTCT ACACATTATC 
TTTATGAATG CAACCATACT GGGCGATGTT ATTTGCAGGG ATTACAGTTC CCGTTTGGCG 
GAGATCGCAA AAGATGCAGT GAGTCATGGA GACGCTGTTA ATATCACGGG GCACAAACAC 
TTCCAGGCAC CTTTGACTGT TCAAGCACTT CAAGCCAATG AAATAAACGG AGTACCTGTT 
AGTGAAATAG TGGTGAAATC AAACACCACC CAAAACTTTA CGGGTACAAA GACCTTTGCC 
CGTGTGGTGG TGGCGAATGA TATCAACGTT CATGATCATT TGAATGTGAG TCATCTTAAT 
GGATTGCCAT TGCAACAGCT GCTTGGTCAC GATATCAGCC TGCAACGCCT CGAACTAACC 
GAAACCCCAC ATCTGGAGAA TCTTCATTTT CGGCGACTTA ATGGATTGCC CTTCGATGAA 
CTGCTGTCCA AAATTTCGGA AGATGGTGAA CAGCCCCTAC TTCTTCACAA GCAACTATTG 
ATCGAAGGAA ATGTACGATT TGACAAGGCT GTCCAAGTGC AGAACATTAA TGGAATCCAA 
TGGGACGAGT ACCTAGATCG CCTGGTGAGA TCTGATTCTA ACGCGGAAAT ACGGGGTAAA 
AAGACATTCA TGGCCGATGT CCAAATAAAT GATGCCCTGC AAAGTCCTCA CATCAATAAT 
TTGGATCTAA GTTCCCTGCT CGACAACACC TTACTGCGAA TGACACCCCA GCAAATCAGT 
GGTGCTTACA CTTTCGACCG CATGATGGCC ACCAATGTGG ATGTAAATCG AATAAATGGG 
GTACCCAAGG AGGAATTTAT AGACACACGC AAAGACTTAG AACTAAAAGG AGATCTTTAC 
CTAAAGCAAC TGAACATCAA TGGCAGCCTA AAGTGTCCCT TTGTCAATGA GGCAGTTTTA 
AATGATCTCA AGCAACGAGT GGAGCAAGTG CAGCAACTTC CCTGGCGGAA TTTGATAGTC 
ACCGGCGATG CTCTATGGGA TGGTGAAGAG CAGTCCCAAT CCCAGCTAGA CTACCTACGC 
CAGCATGCAG TCAGGAGAGT GGGCAACCAG ACCATTGCCG GACACGTTCT ACTGAAGGAA 
CCCTACTTAA AGGATATCCA AAGTAAGCAT TCCCTGCCAA TTGATCTTAA CTTCACTCAA 
TTGGCCGAAG ATGCCCTACT TCGCCGGAGC CCCACAAACG AAACCCAGGT GATAACAGCT 
CCGCAAGAGC TACAGGGATT AGTTAGAGCT AATACTCTAC ACTTGGAGCA GGATGCACAC 
TTTGAGCTGG TAAACGGAAT CGATATACGA CGATTCAATG CCTCACTGTA CCGCCTCTCC 
AGTGGAGAAG CCATTGCAGC GGATCTTCAC TTTCATCAGG CTCCAAAAAT AGGCAAACTT 
CAACTGGAGT ATCCAGAAGT GAATGGAGCT CCGATACAAA GTATATACCA GCAGGGCAAG 
GGGCAATCCT GGCCACCCGT AACGTTCAAG AACCTCTTTG TCGAACAGGA CCTGAATCTG 
AAAACCGTAA ACAACATGAA TCTGGACTAC TTTCTACAAC ATCGCATTCC CTTAAAGGGA 
GACGCATTGG AGGTATTCGG TTCACTGACT TTTGAGCACC TTCAGCTGAG TGGAAAGCCT 
CTTCTACGTT CTATCAATGG CATTCAATTG GATAACTTGG TGTTTCGGCA TAGCAATCGG 
ACGCAGAGCA TCACGGGTGC CAAGACTTTT CGTGGAGGAG TGGAGTTCAC TGGACCCGGT 
CATGTGATGA ATCTGAATGG ACGTGAGCTT AGCGAAAGTT TCAGGGGCAG CATCTTTCGA 
AATAAGGACT ACAACATCGA TAGTCTAGTT CTGGACAAAG CTCTCTTCCC AGGTGGACTA 
GTTTTAGCAG GACTTCAAAG TCAAATTCCC AATCGAATGC GAGCGACGGA AGTGCAAAGC 
AATCCGTTAG AGGAGTTTCA AGAACTTCTA GCATTGGGTA ATCAAACTTC GCCTAGGAGG 
CTACTTTACC TGGATCACGA CACCGAAAGT CTGGAGTCTA CTTGGGTAAA AGGCCCTTTT 
AGGGGATCTT CGGAACTGCA GGTGAGCCTG CCCTTGGGTT ATTCTGCTCC ATGTCAGCGT 
AGGACTTTAC AAGCCAAGCT CCTGATCCAC GAACGTCGCG TCCTCCTGGC CAACTCGAGT 
GTGTCCAATC AACTGATGAG ACTTCACTCC GGAGATATCC GGGTGAAGGT GCAGAACCAC 
TGTCATCGCC CAGCACGAAG ATTGCGCAGC AGGATCAGAA TTAGTTGCCG TAATGAAACG 
CATACGCTGG GAATGCGTCA ATCAGTGGAA GCCATGGAAC TGCTGGAAAT GGGGGAAATG 
GTGCTGCTGC TCTTGGGCAC CGAGGAGGAA GTTCGTGTCC TGCGTCTGAA GAGGAGCAAC 
TGTAGTCTCA CCGATTGGCA ATCCGTGCTA CCAGCAGATG GGCGCCTTAT GAAGTTGGTT 
CGAATGGGGG TAGAGTCCCG ACAGGATCTA ATACTCACCA GTGGCATGAG TAATCATCGT 
CCTGTCCTGG CCATCCATTA TAGGGATCCC TTAAATCATA ACTTCAAAAT GCTTCAGCTG 
ATCCAGGGCT CATATGACCT GGCAGAGATG CAGGGAAACC AACTTCTGGT CAGCTGTTTG 
GGTTGTCGAC ACATAGCTAT CTTTAAACGG TACTCTCATT CCGCGGAAAT TCTGTTTGAG 
CCCATCCAGC AGTTGAGCTT CCAGGAGCGC ATTCAACAAC TAACTCCATT CAAAGTTGGA 
AATGAGCAAT ATCTATTGGT AGTAACGCAG CCGGAGGAAG AACACTTTTA CCTCTTCAGC 
TATAATGAAG TAGGCGGTTG GCAACAGCGT ACGTTTGGCA TTAAGAAACA GGACCAGTGG 
GCGTGGTCAC TGGTGAAATC TGGTCAAATG CTTGAAGCCG CCGAGACCCC TATTCTGCTG 
CTCTGCGGAA AGAAACGGGA ATGTAGTCTA GTGAAAGCCC TACTGGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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fs(1)M3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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